Reproduction in Domestic Animals最新文献

{"title":"Understanding the Crucial Role of Seminal Plasma Exosomes in Bull Fertility: A Review.","authors":"Manisha Sethi, Tushar Kumar Mohanty, Nadeem Shah, Mukesh Bhakat, Nishant Kumar, Rubina Kumari Baithalu","doi":"10.1111/rda.70000","DOIUrl":"10.1111/rda.70000","url":null,"abstract":"<p><p>Bull fertility is a multi-factorial trait and is affected by many factors, such as nutrition, genetics, and epigenetics. Superior quality male germplasm with high genetic merit helps to improve the livestock production trait. To achieve the target of livestock production, the availability of superior male germplasm is a great concern. In developing countries, there is a gap between the highly fertile frozen semen doses produced and the highly fertile frozen semen doses required. Improving the quality of existing low-fertile semen from high genetic merit bulls seems to play a promising role in filling this gap. Seminal exosomes are extracellular vesicles secreted by the epithelial cells of the testis, epididymis, and accessory sex glands such as the prostate gland. They contain a cargo of bioactive molecules such as proteins, nucleic acids and various metabolites. These molecules are transferred to the spermatozoa during its maturation and help in sperm capacitation, maturation, acrosome reaction, and fertilisation. Studies reveal that seminal exosomes help to improve the sperm functionality of low-quality sperm. Identification of the molecular profile of exosomes of bulls with proven fertility and their addition in an extender containing low-fertile semen may help to ameliorate the sperm quality of low-fertile semen, which may eventually aid in generating quantities of highly fertile ejaculates.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 12","pages":"e70000"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Cholesterol Carriers and Substitution of Fructose and Glycerol With Trehalose on Frozen/Thawed ATP Content, DNA Integrity and Kinematics Variables of Ram Spermatozoa.","authors":"Kave Koorehpaz, Mohsen Eslami, Farhad Farrokhi-Ardabili","doi":"10.1111/rda.14748","DOIUrl":"https://doi.org/10.1111/rda.14748","url":null,"abstract":"<p><p>Current study was aimed to assess the β-cyclodextrin (βCD) and methyl-β-cyclodextrin (MβCD) on delivery of cholesterol, and substitution of fructose and glycerol with trehalose on the ram semen cryosurvival. Samples were collected, diluted with Tris-citric acid-LDL extender, pooled, and used. In experiment I, βCD and MβCD carriers were used and compared to deliver the cholesterol (at 0, 0.5, 1, 1.5, 2, and 4 mg/mL). In the experiment II, trehalose (0, 7, 14, 21, and 28 mM) was substituted with fructose (28, 21, 14, 7, 0 mM, respectively). In the experiment III, the best cholesterol/carrier dose groups from the first experiment, were selected to be evaluated with the fructose/trehalose (14/14 mM) combination compared to fructose (28 mM) alone. The concentration of glycerol in the above-mentioned experiments was set at 4.5%. In the experiment IV, the effect of lowering glycerol (4% vs. 4.5%) was assessed using selected cholesterol/carrier groups. Kinematics, chromatin integrity, ATP contents, malondialdehyde amounts and viability were evaluated. Cholesterol (especially at 1.5 and 2 mg/mL) improved the kinematics and ATP levels using both carriers. The optimised amounts of trehalose (14 mM)/fructose(14 mM) reduced peroxidation and DNA fragmentation levels. Co-administration of optimised levels of cholesterol with trehalose/fructose did not show extra beneficial effects compared to each of them. Trehalose could not protect the spermatozoa at lower amounts of glycerol (4% vs. 4.5%). In conclusion, either the optimised levels of cholesterol (using βCD or MβCD carriers) or substitution of half of the fructose with the trehalose alone could lead to improvement in quality of frozen/thawed ram semen.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 12","pages":"e14748"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycosylation in Canine Placentas.","authors":"Carolina N Zanuzzi, Gimena Gomez Castro, Rocío Hernández, Romina Tozzi, Juan E Falcón, Claudio G Barbeito, Mónica E Diessler","doi":"10.1111/rda.14750","DOIUrl":"https://doi.org/10.1111/rda.14750","url":null,"abstract":"<p><p>This article describes the carbohydrate composition of early and mature placentas from bitches, detected by lectin histochemistry. Formalin-fixed placental samples from 11 mixed-breed bitches have been assigned to the 'early' or the 'mature' placenta group, processed by the routine histological technique and labelled with a panel of 14 biotinylated lectins. The glycan distribution was almost completely preserved over pregnancy. Cytotrophoblast cells expressed high-mannose N-linked residues and N-acetyl-D-glucosamine, whereas mannose N-linked residues, N-acetyl-D-glucosamine and β- and α-D-galactose/N-acetyl-D-galactosamine have been found in the syncytiotrophoblast. The maternal and foetal endothelial binding pattern was enriched in β-D-galactose, α-D-mannose in non-bisected bi/tri-antennary N-linked complex and Galα1,3Gal-Galα1,4Gal. Both deep and superficial glands showed a great variety of glycoconjugates, such as D-mannose, D-glucose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, galactose, N-acetylneuraminic acid and fucose. Most results in this study were consistent with those previously reported in canine and feline mature placentas; here, early placentas have been analysed and the lectin binding pattern of mature placentas has been further described. These studies on canine placentas contribute to detecting and understanding glycome changes in pathophysiological conditions.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 12","pages":"e14750"},"PeriodicalIF":1.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In Vitro.","authors":"Yuan Tian, Peng Zhang, Kemin Jing, Yuqian Li, Binglin Yue, Zhijuan Wu, Wenjing Dong, Jincheng Zhong, Xin Cai","doi":"10.1111/rda.14738","DOIUrl":"10.1111/rda.14738","url":null,"abstract":"<p><p>As the hybrid between cattle and yak, cattleyak is a typical male sterile mammal, and the underlying mechanism for its spermatogenic arrest is still unclear. In this study, the coding region of cattleyak TAF4B gene was cloned by RT-PCR and analysed by bioinformatics. To investigate the effects of TAF4B on cellular proliferation and differentiation, an expression vector was generated and introduced into undifferentiated spermatogonia (UDSPG) of cattleyak. The results showed that the protein encoded by TAF4B did not contain the signal peptide sequence. The expression level of TAF4B in UDSPG of cattleyak was lower than that in yak, while the overexpression of TAF4B in cattleyak promoted the proliferation activity of cattleyak UDSPG. Meanwhile, the expression of proliferation and meiosis-related genes was increased but the differentiation-related genes were decreased. Therefore, the aberrant expression of TAF4B in cattleyak UDSPG possibly impaired its proliferation and differentiation equilibrium and decreased its growth potentiality, thereby reducing the quantity of UDSPG and affecting spermatogenesis. This study provided a potential approach for further elucidation of the mechanism of spermatogenesis arrest and provided a new idea for solving the problem of male infertility in cattleyak.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14738"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Indicators of Cryotolerance and Fertility in Bovine Sperm: Evaluating Fresh Semen Quality to Improve AI Outcomes With Frozen-Thawed Sperm.","authors":"Carolina Tamargo, Ferran Garriga, Marc Yeste, Elisabeth Pinart, Rodrigo Muiño, María Teresa Carbajo, Carlos Olegario Hidalgo","doi":"10.1111/rda.14742","DOIUrl":"https://doi.org/10.1111/rda.14742","url":null,"abstract":"<p><p>The success of artificial insemination (AI) with frozen-thawed semen in cattle is influenced by both female factors and sperm quality. In terms of sperm quality, prior studies indicate that the ability of frozen-thawed bovine sperm to fertilise an oocyte is dependent on their quality and resilience to cryopreservation. Cryopreservation induces oxidative stress, leading to ultrastructural damage in the sperm. This study aimed to determine whether the quality of fresh semen can identify bulls with good and poor sperm freezability. This difference between fresh and frozen semen from the same bull allows us to predict fertility. Motility and kinetic parameters were assessed using computer-assisted sperm analysis (CASA), while six functional variables were evaluated through flow cytometry, both before and after the freeze-thaw process on the sperm from 13 bulls. In vivo fertility was measured using 90-day non-return rates. The principal component analysis (PCA) of eight sperm variables post-thaw identified one principal component explaining 81.19% of the total variance and classified the bulls into two groups: Poor freezability bulls (progressive motility: 48.12% ± 8.41%; viability: 77.51% ± 7.61%) and good freezability bulls (progressive motility: 58.64% ± 6.64%; viability: 88.12% ± 2.52%). Bulls with higher freezability showed better sperm viability and motility, as well as lower levels of ROS, superoxides and intracellular calcium before cryopreservation that were significantly correlated with higher non-return rates (NRR). The results underscore the importance of assessing the quality and functionality of fresh semen to predict the fertility potential of cryopreserved sperm. This approach can aid in selecting ejaculates with the best potential for successful artificial insemination, ultimately improving reproductive performance in dairy cattle.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14742"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Different Concentrations of Melatonin on the Quality of Frozen and Thawed Semen of Jamnapari Goat.","authors":"Kabir Alam, Sushant Srivastava, Sanjeev Kumar Verma, Abhishek Kumar Verma, Saurabh, Rajesh Kumar, Rabindra Kumar, Ankit Jaiswal, Azeer Alam, Tanmoy Rana","doi":"10.1111/rda.14743","DOIUrl":"https://doi.org/10.1111/rda.14743","url":null,"abstract":"<p><p>Antioxidants help safeguard sperm cells from damage during the freeze-thaw process. Melatonin and its metabolites have an antioxidative effect. The current study aimed to evaluate the impact of melatonin supplementation in tris-based extenders at various concentrations (0.5, 1.0 and 2 mM) on the freezability of Jamnapari goat spermatozoa. A total of 48 ejaculates were collected twice a week from four Jamnapari bucks (n = 12 × 4) using an artificial vagina during the period of October to November 2023. Selected ejaculates diluted with tris-citric acid egg yolk extender were divided into four equal aliquots, and melatonin (dissolved in 0.1% dimethyl sulfoxide) was added later at 0.5, 1.0 and 2.0 mM to the control group (C) (extender and 0.1% DMSO) and treatment groups T1, T2 and T3, respectively. Various seminal attributes such as progressive motility, livability, acrosomal integrity, sperm abnormalities, sperm plasma membrane integrity, sperm penetration distance by vanguard spermatozoa in polyacrylamide gel and seminal plasma enzyme leakage (AST, ALT, ACP and AKP) were evaluated at post-dilution and post-thawed stages. Our findings revealed that all semen quality parameters were superior in melatonin-treated groups than the C, and the differences were noticeably higher in the T2 group (1.0 mM) than the other groups. Adding 1.0 mM melatonin proved to be the most effective to safeguard sperm cells from cryopreservation induced cryodamage of Jamnapari buck.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14743"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endogenous Tissue Inhibitor of Metalloproteinase-2 Levels Are Associated With High-Quality Neat Semen but Unrelated to Sperm Cryoresistance in Bulls.","authors":"M Pande, S Kumar, S Tyagi, A S Sirohi, N Chand, Y K Soni, S Mahajan, S Saha, A Sharma, Sarika, J S Rajoriya, Anjali, A K Mohanty","doi":"10.1111/rda.14741","DOIUrl":"https://doi.org/10.1111/rda.14741","url":null,"abstract":"<p><p>Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) is part of the tissue inhibitors of the metalloproteinases (TIMPs) family. Its primary function is to regulate the activity of matrix metalloproteinases (MMPs) across various tissues, including those of the reproductive system. This study aimed to quantify the natural levels of TIMP-2 in seminal plasma (SP) and sperm membrane (SM) of bulls, explore potential associations between TIMP-2 levels and semen quality parameters, and examine the relationship between TIMP-2 levels and sperm cryoresistance in bulls. Thirty semen samples from Frieswal breeding bulls were categorized into two groups based on their initial progressive motility (IPM): Good (IPM ≥ 70%; n = 21) and Poor (IPM ≤ 40%; n = 9). The samples were evaluated for their quality parameters at the fresh stage, and TIMP-2 levels were measured in SP and SM using a bovine-specific ELISA kit. Following cryopreservation of Good samples (n = 21), post-thaw motility (PTM) was used to further classify samples into Freezeable (PTM ≥ 50%; n = 14) and Non-Freezable (PTM < 50%; n = 7) groups. In frozen-thawed samples, sperm attributes, kinetics, and functional parameters were assessed, and the results were correlated with retrospective TIMP-2 levels of SP/SM. Our study revealed that the quantified levels of TIMP-2 ranged from 100.27 to 535.95 ng/L in SP and from 0 to 115.78 ng/10 million spermatozoa in SM. TIMP-2 levels in both SP and SM were significantly higher in Good ejaculates compared to Poor ejaculates (p < 0.01). Furthermore, total TIMP-2 levels in the SP/SM of semen samples from bulls showed a positive correlation with fresh semen attributes. However, SP/SM TIMP-2 levels in the Freezeable group did not show any significant differences compared to the Non-Freezable group in post-thaw semen quality attributes, kinetic parameters, and functional tests, except for a significant positive correlation (r = 0.530, p < 0.05) between sperm DNA integrity and SP-TIMP-2 levels. In conclusion, the findings suggested that TIMP-2 can be a positive regulator of semen quality at the neat stage. However, when it comes to the resilience of sperm to cryopreservation, the levels of TIMP-2 do not seem to exert any significant influence in breeding bulls.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14741"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Injectable Progesterone as a Pre-Synchronisation Strategy in a Timed Artificial Insemination Protocol Based on Gonadotropin-Releasing Hormone and Progesterone in Bos indicus Beef Cows in Anoestrous.","authors":"Matheus Cruz Silva, Mariana Moreira Dos Anjos, Higor Souza de Camargo, João Paulo Mendes Lollato, Elis Lorenzetti, Thales Ricardo Rigo Barreiros, Marcelo Marcondes Seneda, Fábio Morotti","doi":"10.1111/rda.14745","DOIUrl":"10.1111/rda.14745","url":null,"abstract":"<p><p>This study evaluated the effects of pre-synchronisation with injectable progesterone (P4) on the ovarian follicular dynamics of Bos taurus indicus cows in anoestrous treated with a timed artificial insemination (TAI) protocol. Multiparous Nelore females (n = 47) at 30-60 days postpartum were used in this study. 10 days before (D-10) the TAI protocol, antral follicle count (AFC; follicles ≥ 3 mm), ovarian condition and body condition score (BCS; 1-5) were assessed and were randomly allocated into two groups: Pre-sync (n = 25), which underwent pre-synchronisation with 150 mg of injectable P4 intramuscularly (i.m.), and control (n = 22), which received the same volume of NaCL 0.9%. On D0, the ovarian assessment was repeated, and TAI protocol was initiated in all animals, with the insertion of an intravaginal P4 device and administration of 10.5 μg of buserelin acetate (gonadotropin-releasing hormone-GnRH). On D7, the P4 device was removed, and 300 IU of equine chorionic gonadotropin, 150 μg of D-cloprostenol and 1 mg of estradiol cypionate were administered i.m. On the same day (D7), the presence of the corpus luteum (CL) was assessed, the dominant follicle was measured, and the tail was painted to evaluate estrous expression. On D9, the largest follicle was remeasured, and TAI was performed. Animals that were not detected in oestrous at the time of AI were administered 10.5 μg of GnRH i.m. Numerical data were analysed using the Mann-Whitney U test. Binary data were analysed using the Fisher's exact test (5%). BCS, both at the beginning of pre-synchronisation (p = 0.45) and TAI protocol initiation (p = 0.20), and AFC (p = 0.36) did not differ between control and Pre-sync groups. The diameter of the largest follicle was similar between the control and Pre-sync groups on D-10 (p = 0.32), D0 (p = 0.33), D7 (p = 0.29) and D9 (p = 0.22). On D7 of the protocol, the Pre-sync group had a higher percentage of CL visible on transrectal ultrasonography (84.0%; p = 0.02) than the control group (54.5%); however, the expression during oestrous did not differ between groups (p = 0.59). The pregnancy rate was similar (p = 0.64) between groups and was not influenced by the CL rate on D7 (p = 0.48), oestrous expression (p = 0.20) or their interaction (p > 0.1). Pre-synchronisation effectively increased the proportion of cows with CL on D7 without altering the diameter of the largest follicle, oestrous expression or pregnancy rate in anoestrous cows treated with a GnRH/P4-based TAI protocol.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14745"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementing the Beltsville Extender With Mitoquinol Improves the Quality and Fertility Potential of the Rooster's Cooled Sperm.","authors":"Reihaneh Nateghi, Reza Masoudi, Nader Asadzadeh","doi":"10.1111/rda.14740","DOIUrl":"https://doi.org/10.1111/rda.14740","url":null,"abstract":"<p><p>Supplementing freeze diluents with certain antioxidants can maintain the quality of chilled sperm. The present study was an attempt to investigate the effect of Beltsville extender supplementation with the mitochondrial-targeted antioxidant 'Mitoquinol' on the quality parameters and fertility potential of rooster sperm during the cooling process. Semen samples were diluted in Beltsville extender, divided into five groups, and supplemented with 0, 1, 10, 100 and 1000 nM Mitoquinol. Samples were stored at 5°C for up to 50 h and then assayed for sperm motility, viability, mitochondrial function, membrane integrity and malondialdehyde concentration after 0, 25 and 50 h of cooling. To assess reproductive performance, artificial insemination was performed using sperm cooled for 25 h. The results showed no differences between groups at the beginning time. Extender supplementation with 10 and 100 nM Mitoquinol resulted in an improvement in total motility, progressive motility, membrane integrity, mitochondrial function and viability (p ≤ 0.05), as well as a lower malondialdehyde concentration (p ≤ 0.05) in comparison to the other groups during 25 and 50 h storage. Fertility rates were higher when roosters were inseminated with semen samples supplemented with 10 and 100 nM Mitoquinol, compared to the control group. Therefore, supplementing Beltsville extender with Mitoquinol (10 and 100 nM) effective in improving the quality and fertility potential of cooled rooster sperm.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14740"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal-Fetal Blood Flow and Progesterone Concentration in Ewes Sheared at Mid or Late-Pregnancy.","authors":"Mariana Garcia Kako Rodriguez, Juan Pedro Bottino, María Jesús Frisch, Marcelo Ratto, Karina Neimaur, Luis Cal-Pereyra, Aline Freitas-de-Melo, Rodolfo Ungerfeld","doi":"10.1111/rda.14744","DOIUrl":"https://doi.org/10.1111/rda.14744","url":null,"abstract":"<p><p>These studies aimed to determine if shearing ewes at the second or last third of gestation modify the uterine and placentome blood flow, placentome size, and maternal progesterone concentration. Pregnant ewes were assigned to four groups of 12 ewes each according to the gestation period: mid-pregnancy sheared (on day 90 of pregnancy) or unshorn group; and late-pregnancy sheared group (on day 121 of pregnancy) or unshorn group. In both experimental periods, using spectral Doppler ultrasonography, placentomes and uterine artery blood flow and placentome size were evaluated 14 days before and 6 days after shearing. An additional measurement was performed 26 days after shearing in mid-pregnant ewes. Serum progesterone concentration was measured before shearing 4, 24, 72 h, and 22 days after shearing. The uterine artery's end-diastolic velocity (EDV) tended to be greater in the sheared than in the non-sheared ewes (p = 0.1). Peak systolic velocity (PSV) and EDV of placentome increased (p = 0.05 and p = 0.008, respectively) on day 26, accompanied by an increase in placentome area (p = 0.035) in mid-pregnant ewes. In late-pregnant ewes, uterine artery and placentome blood flow and size did not vary. Progesterone concentration varied with time but was not affected by shearing. In conclusion, shearing triggered an increase in placentome size and some changes in blood flow only when ewes were sheared during the second third of their pregnancy. Shearing ewes either the second or last third of gestation did not affect uterine artery blood flow and progesterone secretion.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 11","pages":"e14744"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}