Reproduction in Domestic Animals最新文献_第9页

Regional Structural and Functional-Morphological Differences in the Epididymis of the Adult Domestic Cat (Felis catus): Light and Scanning Electron Microscopic Study. 成年家猫附睾的区域结构和功能形态学差异：光学和扫描电镜研究。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70257

Banu Kandil, Beste Demirci

{"title":"Regional Structural and Functional-Morphological Differences in the Epididymis of the Adult Domestic Cat (Felis catus): Light and Scanning Electron Microscopic Study.","authors":"Banu Kandil, Beste Demirci","doi":"10.1111/rda.70257","DOIUrl":"https://doi.org/10.1111/rda.70257","url":null,"abstract":"The current study aimed to characterize the regional histological, histometric and ultrastructural features of the epididymis in adult domestic cats (Felis catus) using light and scanning electron microscopy (SEM). Eight adult cat epididymis samples (2 for SEM, 6 for light microscopy) were used as tissue material in this study. Epididymal samples were subdivided into six regions and examined following routine histological, histochemical and ultrastructural procedures. Histometric measurements included tubular diameter, luminal diameter, epithelial height, stereocilium height and smooth muscle wall thickness. Tubular and luminal diameters, as well as smooth muscle wall thickness, were significantly greater in region VI, whereas stereocilium height was highest in region I and epithelial height was lowest in region VI (p < 0.05). The tubules were lined with pseudostratified columnar epithelium with stereocilia. Basal, principal, narrow and apical cells were identified in the epithelial layer. Intraepithelial glands were identified in regions IV, V and VI. Spermatozoa were present in the lumen of all regions, with a greater visual accumulation in the distal segments. Secretory vesicles and macrophages were also detected in the lumen. Luminal macrophages were more abundant in regions IV, V and VI. Spermatozoa were observed to be embedded within stereocilia, and this morphological pattern was more frequently detected in region VI. PAS staining demonstrated positivity in stereocilia, narrow cells and secretion-filled intraepithelial glands. As a result, the findings demonstrate distinct region-specific morphological and histometric characteristics along the cat epididymis. These structural differences among epididymal regions may reflect variations in regional activity along the epididymal duct.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70257"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-Temperature Equilibration Impairs Follicular Preservation in Vitrified Domestic Cat Ovarian Tissue. 低温平衡对玻璃化家猫卵巢组织卵泡保存的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70239

Olga Rodak, Isa Mohammed Alkali, Manuel David Peris-Diaz, Aleksandra Piotrowska, Agnieszka Partyka, Piotr Dzięgiel, Gaia Cecilia Luvoni, Wojciech Niżański

引用次数: 0

Y-Sperm Enrichment Through TLR 7/8 Validated Through Molecular and Biochemical Approaches in Sahiwal Bull. 通过分子和生物化学方法验证了Sahiwal牛通过TLR 7/8富集y精子。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70261

S Nistane, N Srivastava, A Jackson, N Biswas, K Singh, S Parmar, U Yadav, P Srivastava, L Yadav, S Kumar, A Priyadershi, M H Khan, P Singh, S K Ghosh, P Wyavahare

{"title":"Y-Sperm Enrichment Through TLR 7/8 Validated Through Molecular and Biochemical Approaches in Sahiwal Bull.","authors":"S Nistane, N Srivastava, A Jackson, N Biswas, K Singh, S Parmar, U Yadav, P Srivastava, L Yadav, S Kumar, A Priyadershi, M H Khan, P Singh, S K Ghosh, P Wyavahare","doi":"10.1111/rda.70261","DOIUrl":"10.1111/rda.70261","url":null,"abstract":"Earlier studies reported the X-linked Toll-like receptors 7 and 8 (TLR7/8) in goats, dogs and mice spermatozoa. Conversely, Resiquimod R848, a TLR7/8-agonist, specifically suppresses the motility of X-spermatozoa. Basing above, this study focused on developing a simple immunological approach for producing Y-enriched Sahiwal bull semen using R848, validated through molecular and biochemical assays. Each ejaculate (N = 24; 8 ejaculate × 3 bulls) was divided into Group-I (Control, routine processing) and Group-II (Resiquimod-based sorting) before cryopreservation. Sorting efficiency was evaluated and validated by immunofluorescence for localization and qPCR to determine sperm sex-ratio by detecting X- and Y-bearing sperm through marker genes: PLP and SRY, respectively. The study showed that sex-sorting using Resiquimod at a 0.6 μM concentration yields a fraction rich in Y-cells, preserving the viability and motility at the fresh stage in sorted spermatozoa. The acrosome integrity (fresh stage), and plasma membrane- and DNA-integrity (frozen-thaw) did not differ significantly between G-I and G-II. Though the recovery rate was low, the technique achieves a high level of purity (> 78%) in sorted Y-cells. Standard curves for SRY and PLP genes confirmed a natural 1:1 ratio in the unsorted samples. In Y-enriched samples, Y-bearing sperm made up 78.18%, effectively shifting the sex-ratio towards male offspring. The immunofluorescence study confirmed the presence of TLR 7/8 receptors on the cranial head, acrosome and tail region in cattle sperm, revealing fewer than half of the sorted cells showing TLR7/8 expression on the tail, whereas it was nearly half in the unsorted sample. In conclusion, the study shows the effectiveness of Resiquimod-based sorting in harvesting motile, viable Y-cells without significantly affecting quality.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70261"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148309386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive Performance in Dairy Cattle: Determinants of Conception Rate and Economic Consequences in Bangladesh. 奶牛的繁殖性能：孟加拉国受孕率和经济后果的决定因素。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70235

Md Abdur Rouf Rafi, Md Nazmul Hoque, Tonmoy Kumar Das, Md Arafat Hossain, Subrin Jahan Mourin, Danishuddin, Md Azizul Haque

{"title":"Reproductive Performance in Dairy Cattle: Determinants of Conception Rate and Economic Consequences in Bangladesh.","authors":"Md Abdur Rouf Rafi, Md Nazmul Hoque, Tonmoy Kumar Das, Md Arafat Hossain, Subrin Jahan Mourin, Danishuddin, Md Azizul Haque","doi":"10.1111/rda.70235","DOIUrl":"10.1111/rda.70235","url":null,"abstract":"Reproductive efficiency plays an important role in determining milk production and overall farm profitability, particularly in smallholder systems with limited resources. Poor fertility can lead to substantial economic losses. This study investigated factors associated with conception rate and evaluated the economic consequences of reproductive inefficiency in dairy farms across five randomly selected districts in Bangladesh. A cross-sectional study was conducted involving 120 dairy farms and 569 cows, including local, crossbred and Holstein Friesian (HF) breeds. A structured questionnaire was used to collect data on herd structure, breeding practices, veterinary costs, reproductive performance and associated economic losses. The results revealed a significant interaction between breeding method and location, affecting herd composition, reproductive performance and economic outcomes (p < 0.05). The conception rates for crossbred (68.45%) and HF cows (67.27%) were higher than those of the local breed (64.34%). The average number of cows per farm was 4.75, and the average economic loss was 3407 BDT ≈30 USD due to reproductive inefficiency per farm. A strong positive correlation was observed between repeat breeding and economic loss (r = 0.771, p < 0.01), indicating that increased repeat breeding results in higher economic loss. A multiple linear regression analysis showed that indicators of reproductive performance and veterinary costs were significant predictors of economic loss, explaining 81.6% of the variation in economic loss (R2 = 0.816, p < 0.001). Improved breeding management, efficient artificial insemination services and effective veterinary support may improve reproductive success in smallholder dairy systems.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70235"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148144437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of Management Factors and Environmental Conditions With the Number of Liveborn Piglets in a Commercial Pig Farm: A Retrospective Field Study. 商业养猪场管理因素和环境条件与活产仔猪数量的关系：回顾性现场研究。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70258

Georgia Moutsou, Theodoros Ntallaris, Georgios Tsousis, Junwei Li, Ioannis A Tsakmakidis, Athina Basioura

{"title":"Associations of Management Factors and Environmental Conditions With the Number of Liveborn Piglets in a Commercial Pig Farm: A Retrospective Field Study.","authors":"Georgia Moutsou, Theodoros Ntallaris, Georgios Tsousis, Junwei Li, Ioannis A Tsakmakidis, Athina Basioura","doi":"10.1111/rda.70258","DOIUrl":"10.1111/rda.70258","url":null,"abstract":"The achievement of reproductive goals depends, among others, on semen quality, adequate control of environmental factors (e.g., heat stress) and the practice of annual sow replacement. This retrospective study examined the association of semen type (produced on the farm or purchased from artificial insemination centres), previous oestrus return, parity and thermal environment with the number of liveborn piglets. Data from 522 sows (n = 1035) of an industrial Greek pig farm were analysed. Except for parity, no statistical differences were observed in the number of liveborn piglets across the various parameters examined. The number of liveborn piglets was higher for second- to fifth-parity sows compared to both younger and older ones. In conclusion, under the commercial conditions evaluated in the present study, the findings are consistent with previous reports and provide additional field-based evidence supporting current herd-management practices in commercial pig production.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70258"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13308389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148339826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protective Role of Salicylic Acid-Conjugated Chitosan Nanoparticles Against Cryodamage in Buffalo Semen. 水杨酸共轭壳聚糖纳米颗粒对水牛精液低温损伤的保护作用。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70247

Wael A Khalil, Aya A Ismail, Mahmoud A E Hassan, Mostafa A El-Harairy, Mahmoud Moussa, Sameh A Abdelnour

{"title":"The Protective Role of Salicylic Acid-Conjugated Chitosan Nanoparticles Against Cryodamage in Buffalo Semen.","authors":"Wael A Khalil, Aya A Ismail, Mahmoud A E Hassan, Mostafa A El-Harairy, Mahmoud Moussa, Sameh A Abdelnour","doi":"10.1111/rda.70247","DOIUrl":"10.1111/rda.70247","url":null,"abstract":"This study explored the development of salicylic acid-conjugated chitosan nanoparticles (SACNs) and their effectiveness in enhancing the cryopreservation of buffalo sperm. The assessment focused on post-thaw sperm quality and kinematics, microbiota profiles, oxidative stress markers, acrosome integrity, apoptosis, and docking analysis. Semen was collected from eight fertile buffalo bulls via an artificial vagina. Samples were diluted with a Tris-based extender and supplemented with SACNs at concentrations of 0 μM (SACNs0), 5 μM (SACNs5), 10 μM (SACNs10), 20 μM (SACNs20), and 40 μM (SACNs40). Following a standard freezing procedure, sperm quality and kinematic parameters significantly improved in the SACNs10, SACNs20, and SACNs40 groups, with the greatest improvement in the SACNs40 group (p < 0.05). Acrosome integrity was significantly improved in all SACNs-treated semen samples compared with the SACNs0 treatment (p < 0.05). Additionally, total antioxidant capacity and catalase activity were improved, while hydrogen peroxide and MDA levels were noticeably diminished in all SACNs-treated groups (p < 0.05). Although all SACNs concentrations markedly reduced apoptotic markers, caspase-3 activity, and reactive oxygen species, it showed the lowest levels in the SACNs40 group (p < 0.05). Salicylic acid exhibited strong docking interactions with key regulators of mitochondrial function and sperm motion, including UCP1, HSP70, cytochrome c, and the tubulin-colchicine complex, suggesting a multi-target mechanism of action. Furthermore, supplementation with SACNs at 10, 20, and 40 μM significantly declined coliform and total bacterial counts compared with the SACNs0 treatment (p < 0.05). These findings show that SACNs, acting as nano-antioxidant additives, enhance the cryoresistance of buffalo sperm, suggesting their potential to improve assisted reproductive technologies.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70247"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annual Variation in Stallion Testicular Measurements and Its Association With Hormonal Profiles and Semen Quality. 种马睾丸测量的年变化及其与激素谱和精液质量的关系。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70244

T R C Braga, M L Freitas, D M V Martins, M A O Viu, R A Oliveira

{"title":"Annual Variation in Stallion Testicular Measurements and Its Association With Hormonal Profiles and Semen Quality.","authors":"T R C Braga, M L Freitas, D M V Martins, M A O Viu, R A Oliveira","doi":"10.1111/rda.70244","DOIUrl":"10.1111/rda.70244","url":null,"abstract":"In a stallion breeding-soundness examination, testicular volume may be estimated either from total scrotal width or by measuring the height, width and length of both testes. This study aimed to determine whether testicular morphometric parameters obtained by ultrasonography fluctuate throughout the year, and whether these variations affect semen quality and hormone production in stallions located at low latitudes in the Southern Hemisphere. Eight healthy light-breed stallions were evaluated every 15 days for 1 year, encompassing the breeding (October-April) and non-breeding(May-September) seasons. Assessments included testicular ultrasonographic measurements, semen quality, hormonal profiling and thermographic evaluation. Testicular morphometric parameters, particularly total testicular volume and testicular width, exhibited seasonal variation, with greater values observed during the breeding season. A moderate correlation was identified between total testicular width and total testicular volume, indicating that width contributes to testicular volume variation. Despite the increase in testicular volume during the breeding season, total sperm number per ejaculate remained stable throughout the year, and only subtle seasonal changes were observed in seminal and hormonal parameters. Minor and total sperm defects increased during the breeding season, coinciding with higher ambient temperature, the temperature-humidity index (THI) and scrotal surface temperature. Cortisol concentrations were higher during the non-breeding season, suggesting physiological adaptation to environmental conditions. These findings indicate that stallions maintained at low latitudes exhibit limited reproductive seasonality and suggest that seasonal changes in testicular morphometry are more closely associated with thermoregulatory adaptations than with marked alterations in spermatogenic activity.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70244"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Proliferative Potential and Collagen Deposition in Vitrified Canine Testicular Fragments Subjected to Different Cryoprotectant Combinations and Warming Temperatures. 不同冷冻保护剂组合和温度对玻璃化犬睾丸碎片增殖潜能和胶原沉积的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70253

Juliana de Souza Fernandes, Jéssyka Araújo Noronha, Bruna Farias Brito, Gisele Karla Sena Guimarães, Gaby Judith Quispe Palomino, Carmen Patricia Huayhua Llacho, Lúcia Daniel Machado da Silva

{"title":"Evaluation of Proliferative Potential and Collagen Deposition in Vitrified Canine Testicular Fragments Subjected to Different Cryoprotectant Combinations and Warming Temperatures.","authors":"Juliana de Souza Fernandes, Jéssyka Araújo Noronha, Bruna Farias Brito, Gisele Karla Sena Guimarães, Gaby Judith Quispe Palomino, Carmen Patricia Huayhua Llacho, Lúcia Daniel Machado da Silva","doi":"10.1111/rda.70253","DOIUrl":"https://doi.org/10.1111/rda.70253","url":null,"abstract":"Testicular cryopreservation is a promising tool for fertility preservation, with applications in endangered species, prepubertal animals and individuals of high genetic value. This study evaluated proliferative potential and collagen deposition in adult canine testicular fragments subjected to vitrification using different warming temperatures (37°C for 1 min and 50°C for 5 s) and cryoprotectant combinations: ethylene glycol/glycerol (EG/GLY), dimethyl sulfoxide/glycerol (DMSO/GLY) and dimethyl sulfoxide/ethylene glycol (DMSO/EG). Twenty-four testicular fragments (3 × 1 × 1 mm) per dog were collected from 10 adult dogs, vitrified using the needle immersion method and subsequently warmed according to the experimental protocol. Proliferative potential was analysed using AgNOR staining, and collagen deposition was assessed using Picrosirius staining. AgNOR analysis indicated that proliferative potential was maintained across all groups, with no significant differences (p > 0.05), ranging from 2.42 ± 0.05 (EG/GLY37) to 2.71 ± 0.31 (DMSO/EG50). Picrosirius staining revealed increased collagen only in the DMSO/GLY37 group (194.80 ± 17.14, p < 0.05), with a significant increase in type III collagen fibres (p < 0.05). All other groups maintained collagen levels similar to those of the control group (171.20 ± 18.80, p > 0.05). In conclusion, the tested cryoprotectant combinations and warming temperatures adequately preserved proliferative potential, and collagen organization in vitrified adult canine testicular fragments, except DMSO/GLY37, supporting their application in assisted reproductive technologies & species conservation programmes.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70253"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Astaxanthin Concentration in Extender on Sperm Kinetics and Lipid Peroxidation After Cryopreservation in Beef Bulls. 膨化剂中虾青素浓度对肉牛冷冻后精子动力学和脂质过氧化的影响

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70237

Laura Fernanda Sechirolli Silva, Marcelo Sant'Ana Borges, Leticia Padovani Silva, Marina Oliveira Silva, Joice Maria Bazerla Andreta, Camila de Paula Freitas Dell'Aqua, Fabiana Ferreira de Souza, Frederico Ozanam Papa, Maria Eugênia Zerlotti Mercadante, Lindsay Unno Gimenes, Fabio Morato Monteiro

{"title":"Impact of Astaxanthin Concentration in Extender on Sperm Kinetics and Lipid Peroxidation After Cryopreservation in Beef Bulls.","authors":"Laura Fernanda Sechirolli Silva, Marcelo Sant'Ana Borges, Leticia Padovani Silva, Marina Oliveira Silva, Joice Maria Bazerla Andreta, Camila de Paula Freitas Dell'Aqua, Fabiana Ferreira de Souza, Frederico Ozanam Papa, Maria Eugênia Zerlotti Mercadante, Lindsay Unno Gimenes, Fabio Morato Monteiro","doi":"10.1111/rda.70237","DOIUrl":"10.1111/rda.70237","url":null,"abstract":"Cellular damage caused by changes in temperature and osmolarity during cryopreservation primarily results in excessive generation of reactive oxygen species (ROS). These damages compromise the plasma membrane and reduce sperm fertility. Antioxidants help mitigate these effects. Astaxanthin stands out for its high capacity to protect cellular integrity. The aim of this study was to evaluate the effects of adding astaxanthin (ASTX) to commercial extender on the quality of bovine semen after cryopreservation. Four ejaculates were collected from 12 Nellore bulls, totalling 48 samples. Sperm kinetics, concentration and morphology of the samples were evaluated. The semen sample was then divided into three aliquots and diluted in commercial extender: control (without astaxanthin) and addition of 1 μmol (ASTX1) or 2 μmol (ASTX2) of ASTX. After cryopreservation, the samples were re-evaluated immediately after thawing and after 30 min of incubation at 46°C (rapid thermal resistance test) regarding sperm kinetics, plasma and acrosomal membrane integrity, mitochondrial membrane potential and oxidative stress levels (TBARS). Rapid sperm percentage and amplitude of lateral head displacement were higher (p < 0.05) in the ASTX groups than in the control group. Total motility was higher in the ASTX1 group than in the control group. Although there was no difference in plasma membrane integrity, acrosomal membrane integrity or mitochondrial membrane potential between treatments, lipid peroxidation was lower (p = 0.041) in the ASTX1 and control groups than in the ASTX2 group. We conclude that the inclusion of ASTX at a concentration of 1 μmol in the extender improves sperm kinetics after thawing in beef bulls semen.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70237"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Potential of Traditional Chinese Medicine in Animal Reproduction: A Comprehensive Review. 中药在动物生殖中的治疗潜力综述

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-06-01 DOI: 10.1111/rda.70249

Changqing Si, Shuo Liu, Linjie Liu, Zhenbiao Zhang, Lihuan Guo, Mengpo Zhao, Shuang Ma, Yuxiang Shi, Wanyu Shi, Yuming Chen

{"title":"Therapeutic Potential of Traditional Chinese Medicine in Animal Reproduction: A Comprehensive Review.","authors":"Changqing Si, Shuo Liu, Linjie Liu, Zhenbiao Zhang, Lihuan Guo, Mengpo Zhao, Shuang Ma, Yuxiang Shi, Wanyu Shi, Yuming Chen","doi":"10.1111/rda.70249","DOIUrl":"10.1111/rda.70249","url":null,"abstract":"Animal reproductive efficiency underpins food security, yet conventional management relies on hormones and antibiotics, raising concerns over residues and antimicrobial resistance (AMR). Traditional Chinese Medicine (TCM) offers a multi-target alternative, but a critical synthesis linking its mechanisms to species-specific veterinary application is lacking. This review systematically evaluates the therapeutic potential of TCM in animal reproduction and identifies translational barriers. A literature search was conducted in PubMed, Web of Science and the China National Knowledge Infrastructure (CNKI) up to October 2025. Fifteen key medicinal plants were classified into tonifying, blood-activating and heat-clearing categories, with bioactive compounds, target tissues and signalling pathways summarized in structured tables. TCM compounds converge on conserved regulatory networks-including PI3K/Akt, Nrf2/HO-1, NF-κB and AMPK/SIRT1-modulating the hypothalamic-pituitary-gonadal axis, mitigating oxidative and inflammatory stress and improving reproductive organ function across species. Although these mechanisms are conserved, dosage must be empirically re-determined per target species due to differences in digestive physiology. Persistent challenges include standardization, scarcity of large-scale clinical trials and absence of regulatory frameworks. We propose an integrative roadmap combining multi-omics, drug delivery and species-relevant trials to support TCM's sustainable application within a One Health framework.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70249"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148219165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0