Reproduction in Domestic Animals最新文献

{"title":"Oxidative Status of Kangal Ewes Under Accelerated Versus Conventional Reproductive Management: A Cross-Sectional Comparison at Day 40 of Lactation.","authors":"Abdurrahman Takci, Nazlı Ercan, Mehmet Ekici, Tahsin Takci, Mehmet Akif Demirel, Gamze Sonmez Unal","doi":"10.1111/rda.70308","DOIUrl":"10.1111/rda.70308","url":null,"abstract":"<p><p>In sheep farming, distributing reproductive and lactation periods throughout the year is of great importance for increasing farm profitability. Hormone-supported oestrus induction protocols applied during the anoestrous period enable a second pregnancy and lactation to be achieved within the same year. Whether this accelerated management pattern is associated with an altered oxidative status has not been examined in ewes. In this cross-sectional study, 20 multiparous Kangal ewes that had lambed a single lamb in the early months of the year and were subsequently subjected to a short-term (7-day) synchronisation protocol (flugestone acetate sponge, eCG and cloprostenol) during anoestrus, and which entered a second lactation in the same year (Group 1, accelerated management), were compared with 20 contemporaneous ewes that received no hormonal treatment, were not mated and were therefore in the dry period at sampling (Group 2, conventional management). All ewes in Group 1 again reared a single lamb. Blood was collected from both groups on the same calendar date, corresponding to Day 40 ± 4 of the second lactation in Group 1. Serum malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were measured and compared by Mann-Whitney U test. SOD activity was significantly lower in Group 1 than in Group 2 (60.55 ± 0.876 vs. 70.19 ± 0.476 U/L; p < 0.0001), and MDA concentration was modestly higher (10.30 ± 0.363 vs. 8.99 ± 0.619 nmol/mL; p = 0.035); CAT and GPx activities did not differ (p > 0.05). The MDA difference was of moderate magnitude and did not withstand correction for multiple comparisons (q = 0.070), whereas the SOD difference did. Because the groups differed simultaneously in lactational status, hormonal exposure and recent gestation, these differences cannot be attributed to any single component of the protocol, and the single time point precludes inference about the trajectory of oxidative change. What the data show is that accelerated reproductive management returns the ewe to a pro-oxidant lactational state during a period in which she would otherwise be dry and recovering. Whether the repeated loss of this recovery window has cumulative consequences requires a longitudinal, within-animal design. In conclusion, accelerated reproductive management was associated with lower SOD activity and modestly higher MDA at Day 40 of lactation, but did not increase the intensity of oxidative stress beyond the level previously reported for conventional lactation; what this management system changes is the duration of lactational exposure rather than its intensity.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70308"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13484033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148797550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Landscape of Circular RNAs in Testes of Dezhou Donkey During Distinct Developmental Stages.","authors":"Zulfiqar Ahmed, Wei Liu, Farid Shokry Ataya, Zahid Naseer, Mohsin Nawaz, Muhammad Shakeel, Muhammad Zubair, Faisal Ayub Kiani, Xijun Wang","doi":"10.1111/rda.70310","DOIUrl":"10.1111/rda.70310","url":null,"abstract":"<p><p>Genetic selection is crucial to increase the reproductive performance of stud donkeys. Testicular development and spermatogenesis involve numerous genes, including circular RNAs (circRNAs). However, circRNAs in the male reproductive system of donkeys remain unexplored. This study comprehensively analysed testicular samples from Dezhou donkeys at three different age stages for circRNA using transcriptome data. A total of nine Dezhou donkeys were divided into three groups based on their developmental stages: juvenile (two-month-old, n = 3), adolescent (12-month-old, n = 3) and adult (24-month-old, n = 3) donkeys. We identified 12,084 candidate circRNAs, with 6409 circRNAs specific to the juvenile stage, 6616 to the adolescent stage and 10,031 to the adult stage, respectively. Among these, 3908 circRNAs were expressed across all three age stages. Differential expression analysis revealed 2419 circRNAs exhibiting age-dependent expression changes. Eight circRNAs showed consistent differential expression across all age stages, while specific differential expression patterns were observed between different age pairs. Five representative differentially expressed circRNAs were validated by RT-qPCR, and their expression patterns were consistent with the RNA-sequencing results. Moreover, enrichment analyses of differentially expressed circRNA target genes demonstrated significant enrichment in pathways related to male reproductive processes, including sperm egg recognition and sperm binding to the zona pellucida. Additionally, a circRNA-miRNA-mRNA network, comprising 350 circRNAs, 29 miRNAs and 39 mRNAs, is providing insights into the regulatory mechanisms of non-coding RNAs in testes. In conclusion, circRNA expression patterns are involved in the reproductive biology of testicular development and spermatogenesis in Dezhou donkeys.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70310"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13495847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148797612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silydianin Improves the Preservation of Cooled Boar Semen.","authors":"Kelly Vanessa Zapata Carmona, Mauricio Rojas López, Benjamín Alberto Rojano, Giovanni Restrepo Betancur","doi":"10.1111/rda.70301","DOIUrl":"10.1111/rda.70301","url":null,"abstract":"<p><p>Cooled boar sperm is susceptible to oxidative stress, therefore, the addition of antioxidants to the extender has been a recurring alternative. The objective of this research was to evaluate the effect of silydianin (SILYD) on the preservation of cooled boar semen. Fifteen ejaculates from five boar were extended and supplemented with SILYD at 10, 20, 40 and 60 μM. Then, the samples were cooled for five days at 16°C. Every 24 h, sperm motility and kinematic were assessed with a computer-assisted sperm analysis (CASA) system. At 0 and 96 h, reactive oxygen species (ROS) and total antioxidant capacity (TAC) were evaluated by fluorescence microplate reader, and functionality membrane integrity (FMI) was assessed by hypoosmotic swelling test (HOST). Sperm viability (SV), lipid peroxidation (LPO), and mitochondrial membrane potential (ΔΨM) were evaluated by flow cytometry. Linear models were adjusted and the comparison of the means was carried out by the Duncan test. A sperm motility index (SMI) was calculated and regression analyses were performed. SILYD20 increased total motility (TM) at 72 h of cooling, and SILYD10, SILYD20 and SILYD40 improved progressive motility (PM) at 72 h of cooling, compared to the control (p < 0.05). Likewise, all treatments with SILYD increased the beat cross frequency (BCF) after 24 h (p < 0.05); and SILYD10 and SILYD40 increased rapid spermatozoa (RS) after 72 h of cooling (p < 0.05). SILYD10 reached the maximum SMI. In addition, the regression analysis showed that SILYD20 had the lowest rate of decline of SMI during the cooling period (p < 0.05). At 96 h of cooling, all SILYD treatments reduced ROS production. Likewise, SILYD10 and SILYD60 increased the TAC assessed by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay at 0 h, and by the ferric reducing antioxidant power (FRAP) assay at 96 h of cooling, respectively (p < 0.05). At 0 h of cooling SILYD10 produced an increase in FMI (p < 0.05), and a decrease in sperm with non-intact membrane and non-peroxidized lipids (p < 0.05). It is concluded that silydianin improves the quality of cooled porcine semen, and reduces oxidative stress by lowering ROS production and enhancing antioxidant capacity.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70301"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13425257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of BRD0539 on Gene Editing and Mosaicism Rate in Porcine Gene Editing Embryos by CRISPR/Cas9.","authors":"Ruoqian Wang, Xiaoyi Liu, Haoran Li, Yan Wang, Jiancong Zhang, Bingyan Jin, Yunhai Zhang, Huiqun Yin, Yunsheng Li","doi":"10.1111/rda.70255","DOIUrl":"https://doi.org/10.1111/rda.70255","url":null,"abstract":"<p><p>Microinjection is a common method for generating gene-edited animals; however, persistent Cas9 activity post-cleavage often results in mosaic embryos due to editing occurring in different blastomeres. This study investigated whether co-injecting the CRISPR/Cas9 system with the small-molecule Cas9 inhibitor BRD0539, or supplementing it in the culture medium, could reduce mosaicism while maintaining editing efficiency in porcine parthenogenetic activation embryos targeting the myostatin (MSTN) gene. The findings are as follows: Co-injection of 10 or 100 μM BRD0539 with Cas9 mRNA: sgRNA significantly reduced gene editing efficiency (28.5% ± 11.6% and 33.8% ± 4.1%, respectively, vs. 86.9% ± 4.5% in control, p < 0.05). Supplementing the culture medium with 10 or 50 μM BRD0539 also reduced both editing efficiency (20.8% ± 12.4% and 47.7% ± 14.6%, respectively, vs. 85.6% ± 4.8%) and mosaicism rate (25.0% ± 15.9% and 12.5% ± 12.5%, respectively, vs. 87.1% ± 7.8%, p < 0.05). Immunofluorescence revealed sustained Cas9 protein expression up to 48 h post-injection. Crucially, short-term addition of 10 μM BRD0539 to the culture medium between 24 and 48 h post-activation significantly reduced mosaicism (32.6% ± 7.5% vs. 78.7% ± 9.6%, p < 0.05) without compromising editing efficiency. Furthermore, this treatment did not adversely affect cleavage rates, blastocyst development, total cell number. These results demonstrate that transient inhibition of Cas9 activity with 10 μM BRD0539 during a critical window effectively reduces mosaicism in microinjected porcine embryos, offering a promising strategy to enhance the efficiency of generating non-mosaic gene-edited livestock.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 7","pages":"e70255"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Feasibility of a Microfluidic Method Using the ZYMŌT System for Canine Sperm Selection.","authors":"Katarzyna Jaworska, Barbara Smalec, Maria Eberhardt, Joanna Kochan, Wojciech Niżański","doi":"10.1111/rda.70284","DOIUrl":"10.1111/rda.70284","url":null,"abstract":"<p><p>The ZyMōt system is one of the commercially available sperm selection tools based on the microfluidic method. The aim of this study was to evaluate the potential of the ZyMōt system for the selection of canine sperm. Semen samples from six dogs were subjected to selection. Spermatozoa were assessed using basic and advanced methods of semen analysis, including computer-assisted sperm analysis and flow cytometry. Sperm viability, morphology, motility and morphostructural features, such as cell membrane integrity, acrosome integrity and mitochondrial membrane potential, were assessed. The results revealed that the ZyMōt membrane is able to retain dead, slow-moving, immotile and morphologically abnormal spermatozoa. However, no significant improvement was observed in any of the evaluated parameters in the selected sperm population. Moreover, the selection process resulted in substantial losses in the total number of sperm. For this reason, the ZyMōt system is not applicable for dog sperm selection under the currently developed protocol. Further research is necessary to adapt this method for use in this species.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 7","pages":"e70284"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13387722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of Ram Sperm Quality During Chilled Storage by Supplementation With Spirulina platensis Extract.","authors":"H Yousofvand, S M Mousavi","doi":"10.1111/rda.70264","DOIUrl":"10.1111/rda.70264","url":null,"abstract":"<p><p>This study investigated the effects of Spirulina extract, a rich source of bioactive antioxidants, on ram sperm quality during liquid storage. Semen from four mature Lori rams was collected, diluted with a Tris-based extender supplemented with Spirulina extract at concentrations of 0, 10, 20, 30, 40, 50, or 60 μg/mL, and stored at 4°C-5°C. Sperm quality parameters, including motility, viability, plasma membrane integrity, morphology, malondialdehyde (MDA) levels, and antioxidant enzyme activities, were evaluated at 1, 24, 48, 72, and 96 h. A completely randomized design with repeated measures was used, and treatment means were compared using Tukey's HSD test. The 30 μg/mL Spirulina extract (Spir-30) significantly improved total motility (74.17% vs. 63.35% in control, p < 0.05), progressive motility, linearity (28.49%, p < 0.05), straight-line velocity (VSL), and average path velocity (VAP) compared to the control and other concentrations. Plasma membrane integrity was significantly enhanced in Spir-30 (78.77% vs. 58.34% in control, p < 0.05). Additionally, Spir-30 reduced MDA levels (5.32 nmol/mL vs. 7.87 nmol/mL in control, p < 0.05) and increased glutathione peroxidase (GP_X) activity (21.35 U/mg vs. 9.28 U/mg in control, p < 0.05) and total antioxidant capacity (TAC; 4.5 mmol/L vs. 3.14 mmol/L in control, p < 0.05). These findings indicate that 30 μg/mL Spirulina extract optimally preserves ram sperm quality during refrigerated storage by enhancing motility, membrane integrity, and antioxidant defences while reducing lipid peroxidation, offering a promising supplement for improving semen longevity in Lori breed reproductive programs.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 7","pages":"e70264"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics Reveals L-Carnitine to Enhance Semen Quality in Malabari Bucks via Nutrigenomic Regulation of Key Biological Processes Associated With Male Fertility.","authors":"Doddannavar Shashikiran, Muhasin Asaf, Bindya Liz Abraham, Lali F Anand, Senthil Murugan, Kokkadath Naheef, Gongale Tushar Surendra, Jaya Madhuri, Balamurali Vigneshwaran, T S Nisha, Kanjirakuzhiyil Promod","doi":"10.1111/rda.70266","DOIUrl":"10.1111/rda.70266","url":null,"abstract":"<p><p>Male infertility poses a significant challenge in animal breeding, impacting genetic progress and reproductive efficiency. This study investigated the effects of L-carnitine supplementation (300 mg/kg basal diet for 105 days) on semen quality and underlying molecular mechanisms in Malabari bucks using a transcriptomic approach. Eight mature bucks (2-3 years) were randomly divided into control and treatment groups (n = 4 each). Semen parameters like semen volume (mL), concentration (10⁶/mL), viability (%), abnormality (%), acrosome integrity (%), plasma membrane integrity (%) and oxidative stress via the Nitro Blue Tetrazolium (NBT) assay were evaluated. For transcriptomic analysis, sperm RNA was isolated, sequenced on the Illumina NovaSeq 6000 platform (150 bp paired-end), and differentially expressed genes (DEGs) were identified using DESeq2. Functional annotation was performed through GO enrichment and KEGG pathway analysis, whereas hub genes were identified via protein-protein interaction (PPI) analysis using STRING and Cytoscape. A total of 425 DEGs were identified, with 258 upregulated and 167 downregulated. Significant improvements were observed in acrosomal integrity, plasma membrane integrity, and oxidative stress markers following L-carnitine supplementation. GO enrichment analysis suggested upregulation of biological processes associated with meiotic cell division, sperm structural integrity, DNA repair, and spermatogenesis, alongside downregulation of carbohydrate metabolism pathways and a possible shift toward fatty acid oxidation. KEGG pathway analysis indicated upregulated Polycomb repressive complex pathway, and downregulation of insulin resistance and oestrogen signalling pathways, potentially creating a favourable endocrinological environment for male fertility. The hub genes identified, namely PMS1, ZFAND4, MYSM1, EEFSEC and CEP126, were found to be associated with key processes in male fertility. These findings provide preliminary mechanistic insights into L-carnitine's potential therapeutic role in small ruminant reproduction, and further functional validation of the identified hub genes is warranted.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 7","pages":"e70266"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148423717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immuno-Targeting of CLRN3 and SCAMP1 as a Potential Sex Specific Marker in Bovine Spermatozoa.","authors":"Ashmita Debnath, Hemanth Kumar Pundla, Kaivalya Deshmukh, Vineet Kumar Pandey, Neeraj Srivastava, Ajay Kumar, Karuna Irungbam, Subrata Kumar Ghosh, S K Singh, Mukesh Kumar, Sujatha Lakshminarayanan, Bhanu Prakash, Praveen Singh","doi":"10.1111/rda.70267","DOIUrl":"https://doi.org/10.1111/rda.70267","url":null,"abstract":"<p><p>Sex-specific proteins are unique markers found on either X- or Y-chromosome bearing spermatozoa, acting like molecular signatures, and can be used as a key target to distinguish X- or Y-bearing cells. Towards this goal, by reviewing the literature, a total of 6 proteins were selected from the selected, of which 2, CLRN3 and SCAMP1, were finally chosen for the immunoprecipitation study. For the finally selected proteins, predictions of subcellular localisation and transmembrane topology were generated using online tools such as BUSCA, DeepLoc 2.0, DeepTMHMM, and TMHMM. Additionally, several online platforms like SEMMA 2.0 and IEDB were employed to identify potential immunogenic targets. Following this, commercially available antibodies against the 2 identified proteins were procured for subsequent use in multiple immunoprecipitation-based techniques, such as Western blotting, immunocytochemistry, and ELISA (Enzyme-linked immunosorbent assay). Targeting these proteins revealed their binding intensity on spermatozoa. In this study, the CLRN3 antibody targeting X-spermatozoa showed stronger interaction with X-sorted samples than the Y-specific SCAMP1 antibody in the cell-based ELISA. Results from the study suggest targeting sex-specific surface proteins could enable the development of an immune-based sperm-sorting technique in bovines. Overall, the results support the potential of CLRN3 as an X-specific surface marker that needs further validation for the development of a novel immune-based sperm sorting technique.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 7","pages":"e70267"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of an Anti-GnRH Vaccine Improvac on Estrous Activity, and Ovarian and Uterine Morphology in Adult Queens (Felis catus).","authors":"Washington Fernando Carrasco-Sangache, María Carla García Mitacek, María Cecilia Stornelli, Florencia García, Jorge Jagger Segura Ochoa, Rodolfo Luzbel de la Sota, María Alejandra Stornelli","doi":"10.1111/rda.70268","DOIUrl":"10.1111/rda.70268","url":null,"abstract":"<p><p>Reproduction can be avoided using immunocontraception. This research aims to study the effect of the anti-GnRH vaccine Improvac on clinical and reproductive function in colony-housed adult queens (Felis catus). Short-hair mixed-breed queens (n = 12) were housed together under a 14-h photoperiod. The treated group (TRT, n = 9) received 0.5 mL of Improvac, and the control group (CON, n = 3) received 0.5 mL of saline solution. Queens received two doses 30 days (D) apart. Animals were divided into 4 groups (G). Control queens (G1: n = 3) were ovariohysterectomized at 90 D. Treated queens were ovariohysterectomized at 120 D (G2, n = 3), at 270 D (G3, n = 3) and at 330 D (G4, n = 3). Data were analysed with GLIMMIX. The estrus was determined by behavioural observation and vaginal cytology. All CON queens displayed signs of estrus every 12-20 days. In the TRT group, seven queens (n = 7/9) did not display signs of estrus until the day of ovariohysterectomy (120 D [n = 2], 270 D [n = 2], 330 D [n = 3]). However, two queens (120 D [n = 1], 270 D [n = 1]) displayed signs of estrus after receiving the vaccine, at G1 on D10 and G2 on D45. The clinical, haematological and biochemical parameters of treated queens remained within the reference range for the species, supporting short-term tolerability of the vaccine. The TRT queens developed painless, soft tissue masses in the subcutaneous tissue at the vaccination sites. The masses appeared after the first dose but were not detectable at the end of the study. No histological abnormalities were detected in the endometrium or ovaries of control queens at 90 D. However, TRT animals exhibited altered follicular development, characterised by ovarian follicular cysts, while the endometrium predominantly showed atrophic changes. This vaccine is a promising alternative for reproductive control in adult queens.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 7","pages":"e70268"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial Effect of Follicle-Stimulating Hormone Priming on OPU-IVF in Prepubertal Calves of Japanese Black Cattle (Wagyu).","authors":"Zhihui Liu, Huiqiu Zhao, Xiao Chen, Guangyong Xu, Zhentian Liu, Sukun Gu, Zhijia Li, Wenlong Fang, Junbo Zhang, Xuesong Xing, Giorgio Antonio Presicce, Fuliang Du","doi":"10.1111/rda.70242","DOIUrl":"10.1111/rda.70242","url":null,"abstract":"<p><p>Hormonal stimulation was performed on Japanese black prepubertal calves (8- and 12-month-old) and heifers (2-3-year-old), followed by oocyte retrieval by ovum pick-up (OPU) and in vitro fertilization (IVF). Both calves and heifers underwent (i) follicle ablation 36 to 48 h prior to follicle-stimulating hormone (FSH) priming, (ii) hormonal stimulation with 100 IU (calves) or 200 IU (heifers) of FSH twice per day (12 h interval) for 2 days, and (iii) OPU following a coasting period of 40 to 42 h after the last FSH injection. Similar number of calves and heifers served as control (without FSH stimulation) at each session, to their FSH primed counterpart. FSH priming significantly increased the numbers of total antral follicles (calves, 23.5-23.7 vs. 8.6-9.9; heifers, 19.5 vs. 5.4, respectively, p < 0.05) and medium and large follicles compared to control animals. Embryo development of oocytes derived from calves post IVF was significantly increased by FSH stimulation, at cleavage (63.1%-66.1% vs. 41.0%-45.1%), blastocyst development (12.7%-13.2% vs. 1.5%-2.1%), and blastocyst yield per OPU per animal (1.4-1.5 vs. 0.1-0.2 embryos, respectively, p < 0.05). Pregnancy rate from transferred blastocysts derived from FSH primed donor calves was significantly improved to 38.6% compared to untreated donors (38.6% vs. 13.3%, respectively; p < 0.05). FSH priming of 8- and 12-month-old Japanese black prepubertal calves prompted a similar blastocyst development (13.2% vs. 12.7%, p > 0.05) of OPU oocytes post IVF. Although FSH priming was equally effective in prepubertal calves and heifers in increasing antral follicle number and recoverable oocytes, on the contrary IVF outcomes, blastocyst yields and pregnancy, were still lower when compared to heifers. In conclusion, OPU-IVF can be applied to FSH-primed prepubertal calves for rapid enhancement of Japanese black cattle characterised by extremely high genetic merits, by significantly reducing generation interval.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 6","pages":"e70242"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148176232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}