Reproduction in Domestic Animals最新文献_第8页

Number of Blastodisc Cells During the Laying Period for Two Successive Generations of Layer and Broiler Breeder. 连续两代蛋鸡和肉种鸡产蛋期的爆破细胞数

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14721

Ophélie Bernardi, Mathias Fréville, Christelle Ramé, Maxime Reverchon, Joëlle Dupont

{"title":"Number of Blastodisc Cells During the Laying Period for Two Successive Generations of Layer and Broiler Breeder.","authors":"Ophélie Bernardi, Mathias Fréville, Christelle Ramé, Maxime Reverchon, Joëlle Dupont","doi":"10.1111/rda.14721","DOIUrl":"https://doi.org/10.1111/rda.14721","url":null,"abstract":"In the poultry industry, genetic selection for growth performance is associated with poor reproductive efficiency and an increase in embryo mortality. The identification of new biomarkers is essential to improve these parameters. The blastodisc, composed of blastodermal cells, undergoes cellular events to achieve embryo development. Factors such as hen's age, temperature and time of egg storage could influence the number of blastodermal cells and impair embryo development. In this study, we investigated the variability of the number of viable cells of blastodisc (NVCB) that could be dependent on the stage of laying and on the breed and potentially associated with reproductive parameters. In experimental breeds, eggs were collected during the whole cycle of laying. Then, the protocol was repeated on industrial breeds (breeder hens) during five successive days at three stages of laying (before, after laying peak and at the end of laying period) for two generations (mothers and offsprings). For each egg, the blastodisc was dissected in order to count viable cells. For both experiments, the NVCB increased during the laying cycle. The NVCB was higher in broiler blastodisc compared to layer blastodisc for both generations. For layer breed, the NVCB were negatively correlated with laying rate for the first generation while positively associated for offsprings. However, the NVCB was positively correlated with laying rates in both generations for broiler hens and with fertility and hatchability rates. The NVCB from fresh oviposited fertilised eggs could be a potential tool in predicting on reproductive performances in poultry.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 9","pages":"e14721"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Issue: Proceedings of the 27th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), 12-14 September 2024, Belgrade, Serbia. 特刊：欧洲家畜繁殖学会（ESDAR）第 27 届年会论文集，2024 年 9 月 12-14 日，塞尔维亚贝尔格莱德。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14682

引用次数: 0

Contraceptive effect of a gonadotropin-releasing hormone vaccine on captive male brown bears (Ursus arctos). 促性腺激素释放激素疫苗对圈养雄性棕熊（Ursus arctos）的避孕效果。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14707

Naoya Matsumoto, Jumpei Tomiyasu, Kyogo Hagino, Motozumi Matsui, Yojiro Yanagawa

{"title":"Contraceptive effect of a gonadotropin-releasing hormone vaccine on captive male brown bears (Ursus arctos).","authors":"Naoya Matsumoto, Jumpei Tomiyasu, Kyogo Hagino, Motozumi Matsui, Yojiro Yanagawa","doi":"10.1111/rda.14707","DOIUrl":"10.1111/rda.14707","url":null,"abstract":"Fertility control has traditionally been applied in zoos to control captive populations, and reversible contraception is important. However, contraceptive methods for male bears have not been reported. We aimed to establish a reversible contraceptive for male brown bears by investigating the effects of a gonadotropin-releasing hormone (GnRH) vaccine (Improvac®) that was developed for the immune castration of pigs. We vaccinated six bears with two sequential doses of 400 (n = 2) or 600 μg Improvac® (n = 4) with a 1-month interval during the pre-breeding season (February to April). We compared the reproductive parameters (testosterone levels and semen parameters) of the six vaccinated and four non-vaccinated (control) bears once during the breeding season (May or June). To investigate whether the reproductive performance could be restored in the following year of contraception, we also compared the reproductive parameters once during the breeding season in two bears between the year with GnRH vaccination and the following year without vaccination. Vaccination treatments suppressed reproductive parameters in 5 bears, although vaccination with 400 μg of Improvac® was not effective in one bear. Testosterone levels and the rate of progressive sperm motility were significantly lower, and total sperm count and testis size tended to be lower in vaccinated bears, compared with the controls. Blood biochemical findings and direct observations after Improvac® vaccination did not reveal side effects. Moreover, testosterone levels and spermatogenic scores of two bears were restored in the following year. We confirmed that the Improvac® vaccine elicited a reversible contraceptive effect in male brown bears.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 9","pages":"e14707"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends and challenges in liquid-preserved boar semen production: From boar to product. 液体保存公猪精液生产的趋势与挑战：从公猪到产品

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14590

Martin Schulze, Sophie Henneberg, Anine Riedel, Britta Hensel

引用次数: 0

Optimising Laparoscopic Artificial Insemination in Lohi Sheep: Effects of Timing, Sperm Concentration and Body Condition on Fertility Outcome in Subtropical Climates. 优化洛希绵羊腹腔镜人工授精：亚热带气候条件下时机、精子浓度和体况对受精结果的影响

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14725

Muhammad Tayyab Khan, Ejaz Ahmad, Muhammad Rizwan Yousaf, Muhammad Oneeb, Muhammad Saleem Akhtar, Muhammad Irfan-Ur-Rehman Khan

{"title":"Optimising Laparoscopic Artificial Insemination in Lohi Sheep: Effects of Timing, Sperm Concentration and Body Condition on Fertility Outcome in Subtropical Climates.","authors":"Muhammad Tayyab Khan, Ejaz Ahmad, Muhammad Rizwan Yousaf, Muhammad Oneeb, Muhammad Saleem Akhtar, Muhammad Irfan-Ur-Rehman Khan","doi":"10.1111/rda.14725","DOIUrl":"10.1111/rda.14725","url":null,"abstract":"The objective of this study was to determine the optimal timing, sperm concentration, and body condition score (BCS) for laparoscopic artificial insemination (LAI) in the subtropical Lohi sheep breed. In Experiment 1, Lohi ewes (n = 80) were synchronised through progestin-sponges (day 0-day 11), administering PGF2α (d-cloprostenol 75 μg/mL; i.m. @ 75 μg /ewe) on day 09 and eCG (i.m. @ 300 IU/ewe) on day 11. Ewes were divided equally into four groups (n = 20 each) and then LAI was performed at 48 (T48), 60 (T60), 72 (T72) and 96 (T96) hours post-sponge removal using 200 million sperm/insemination. In Experiment 2, ewes (n = 81) were synchronised as in Experiment I. Following synchronisation ewes were divided into four groups, each subjected to LAI employing varying sperm concentrations: 10 (S10; n = 21), 20 (S20; n = 20), 50 (S50; n = 20), or 100 (S100; n = 20) million per insemination. Inseminations were performed within a time window of 48-60 h post-sponge removal, based on the findings from Experiment 1. In both experiments ewes were categorised according to BCS, that is, medium 3 and high > 3. Results of Experiment 1 revealed a quadratic response that ewes inseminated at 60-h post-sponge removal exhibited significantly higher pregnancy and twinning rates (p = 0.02). The results of Experiment 2 revealed that sperm concentration had a quadratic effect, 50 million sperm per insemination resulted in maximal pregnancy rates (p = 0.01). Additionally, ewes with medium BCS (≤ 3) had higher pregnancy rates than high BCS (> 3) ewes. In conclusion, ewes with medium BCS (≤ 3) are ideal candidate for LAI and can be inseminated at 60 h with minimal sperm 50 × 106/dose post-sponge removal to achieve a maximum pregnancy rate.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 9","pages":"e14725"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

G-Protein-Coupled Receptor Kinase 2 Inhibition Induces Meiotic Arrest by Disturbing Ca²⁺ Release in Porcine Oocytes. G 蛋白偶联受体激酶 2 抑制通过干扰猪卵母细胞的 Ca2+ 释放诱导减数分裂停滞

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14715

Ji-Dam Kim, Song-Hee Lee, Xiao-Han Li, Qin-Yue Lu, Cheng-Lin Zhan, Gyu-Hyun Lee, Jae-Min Sim, Hyeon-Ji Song, Dongjie Zhou, Xiang-Shun Cui

{"title":"G-Protein-Coupled Receptor Kinase 2 Inhibition Induces Meiotic Arrest by Disturbing Ca2+ Release in Porcine Oocytes.","authors":"Ji-Dam Kim, Song-Hee Lee, Xiao-Han Li, Qin-Yue Lu, Cheng-Lin Zhan, Gyu-Hyun Lee, Jae-Min Sim, Hyeon-Ji Song, Dongjie Zhou, Xiang-Shun Cui","doi":"10.1111/rda.14715","DOIUrl":"10.1111/rda.14715","url":null,"abstract":"G-protein-coupled receptor kinase 2 (GRK2) interacts with Gβγ and Gαq, subunits of G-protein alpha, to regulate cell signalling. The second messenger inositol trisphosphate, produced by activated Gαq, promotes calcium release from the endoplasmic reticulum (ER) and regulates maturation-promoting factor (MPF) activity. This study aimed to investigate the role of GRK2 in MPF activity during the meiotic maturation of porcine oocytes. A specific inhibitor of GRK2 (βi) was used in this study. The present study showed that GRK2 inhibition increased the percentage of oocyte arrest at the metaphase I (MI) stage (control: 13.84 ± 0.95%; βi: 31.30 ± 4.18%), which resulted in the reduction of the maturation rate (control: 80.36 ± 1.94%; βi: 65.40 ± 1.14%). The level of phospho-GRK2 decreased in the treated group, suggesting that GRK2 activity was reduced upon GRK2 inhibition. Furthermore, the addition of βi decreased Ca2+ release from the ER. The protein levels of cyclin B and cyclin-dependent kinase 1 were higher in the treatment group than those in the control group, indicating that GRK2 inhibition prevented a decrease in MPF activity. Collectively, GRK2 inhibition induced meiotic arrest at the MI stage in porcine oocytes by preventing a decrease in MPF activity, suggesting that GRK2 is essential for oocyte meiotic maturation in pigs.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 9","pages":"e14715"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Periconceptional and Gestational Vitamin D3 Restriction on Fetal Folliculogenesis and Anti-Mullerian Hormone Secretion Using Sheep as a Model. 以绵羊为模型研究围孕期和妊娠期维生素 D3 限制对胎儿蓇葖果生成和抗苗勒氏管激素分泌的影响

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14717

E C Mbegbu, M Salavati, L O Aka, I R Obidike, J C Y Tang, W D Fraser, M A Hanson, L R Green, A A Fouladi-Nashta

{"title":"Impact of Periconceptional and Gestational Vitamin D3 Restriction on Fetal Folliculogenesis and Anti-Mullerian Hormone Secretion Using Sheep as a Model.","authors":"E C Mbegbu, M Salavati, L O Aka, I R Obidike, J C Y Tang, W D Fraser, M A Hanson, L R Green, A A Fouladi-Nashta","doi":"10.1111/rda.14717","DOIUrl":"https://doi.org/10.1111/rda.14717","url":null,"abstract":"Ovarian reserve is a reflection of the overall female reproductive potential. Vitamin D status has been suspected to influence fetal development and female fertility. As maternal diet during pregnancy can affect fetal development and future fertility, we hypothesised that periconceptional and gestational Vitamin D restriction could affect folliculogenesis and AMH secretion in the offspring. Nineteen sexually mature Welsh mountain ewes were randomly assigned to Vitamin D3 deficient (VDD, n = 10) and Vitamin D3 control (VDC, n = 9) diets from 17 days (d) before mating, up to 127-130 days of gestation, when fetal ovaries were collected (3 from VDC and 6 from VDD). Serum 25(OH)D3 concentrations were lower in VDD compared with VDC (p < 0.05). Relative to total follicle number, the percentage of primordial follicles was higher (p < 0.05), while the percentage of primary follicles was lower (p < 0.05) in VDD group compared with VDC group fetal ovaries. The integrated density value and percentage of affected area in TUNEL staining in VDD group did not vary from VDC group fetal ovaries (p > 0.05). Relative expression of AMH mRNA and AMH protein in VDD fetal ovaries were not statistically different compared with controls (p > 0.05). The relative expression of VDR mRNA were lower in VDD compared with VDC group fetal ovaries (p < 0.05). These data indicate that maternal Vitamin D dietary restriction is associated with ovarian tissue stemness and increased primordial follicle number but does not promote normal follicle recruitment or development in sheep fetal ovaries.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 9","pages":"e14717"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Issue: Proceedings of the 27th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), 12-14 September 2024, Belgrade, Serbia. 特刊：欧洲家畜繁殖学会（ESDAR）第 27 届年会论文集，2024 年 9 月 12-14 日，塞尔维亚贝尔格莱德。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14683

引用次数: 0

Genetic and Phenotypic Trends for Production and Reproduction Traits in Murrah Buffaloes. 穆拉水牛生产和繁殖性状的遗传和表型趋势。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14712

Rohit Sharma, Satpal Dahiya, Yogesh Chandrakant Bangar, Renuka Hada, Dipin Chander Yadav

{"title":"Genetic and Phenotypic Trends for Production and Reproduction Traits in Murrah Buffaloes.","authors":"Rohit Sharma, Satpal Dahiya, Yogesh Chandrakant Bangar, Renuka Hada, Dipin Chander Yadav","doi":"10.1111/rda.14712","DOIUrl":"https://doi.org/10.1111/rda.14712","url":null,"abstract":"The objective of the study was to estimate genetic and phenotypic trends for first lactation production and reproduction traits in Murrah buffaloes. The information of pedigree and targeted traits of 640 Murrah buffaloes was collected for the period from 1997 to 2020. The first lactation production traits included first lactation milk yield (FLMY), 305 days first lactation milk yield (305FLMY), first lactation length (FLL), first lactation peak yield (FPY) whereas reproduction traits included first service period (FSP), first calving interval (FCI) and first dry period (FDP). Genetic and phenotypic trends were estimated. Phenotypic trends for FLMY, 305FLMY, FLL and FPY exhibited as 36.96 ± 8.58 kg/year, 31.93 ± 8.34 kg/year, 1.47 ± 0.55 days/year and 0.12 ± 0.02 kg/year, respectively and respective genetic trends exhibited as 3.73 ± 1.67 kg/year, 1.94 ± 0.76 kg/year, -0.15 ± 0.07 days/year and 0.01 ± 0.01 kg/year, respectively. It was revealed that there were significant (p < 0.05) and positive phenotypic trends for all production traits while genetic trends were significant (p < 0.05) for FLMY and 305FLMY traits. The phenotypic trends of studied reproductive traits indicated that only FDP trait significantly (p < 0.01) decreased (1.87 days/year) over time. For FSP and FCI traits, nonsignificant (p > 0.05) genetic and phenotypic trends indicated no change over time. This study highlighted potential challenges in maintaining reproductive efficiency alongside productivity improvements in Murrah buffaloes.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 9","pages":"e14712"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine ART and antral follicle count: Can we deepen our understanding to improve outcomes? 马 ART 和前卵泡计数：我们能否加深理解以改善结果？

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-09-01 DOI: 10.1111/rda.14625

Aurore Le Breton, Niamh Lewis

{"title":"Equine ART and antral follicle count: Can we deepen our understanding to improve outcomes?","authors":"Aurore Le Breton, Niamh Lewis","doi":"10.1111/rda.14625","DOIUrl":"https://doi.org/10.1111/rda.14625","url":null,"abstract":"Assisted reproductive technologies (ARTs) are performed worldwide in the equine industry to produce genetically valuable foals. Among them, ovum pick up (OPU) combined with intra-cytoplasmic sperm injection (ICSI) can now be more efficient than embryo transfer (ET) under optimal conditions. However, OPU is not a benign procedure for the mare and the process is costly. Improved efficiency is therefore in the interest of everyone, maximizing mare welfare and optimizing economics for the client. One of the key factors of success is the antral follicle count (AFC) at the time of OPU and subsequently the number of oocytes obtained. Variations in AFC are reported between individuals and between geographical areas. This leads to a significant increase in numbers of embryos produced per session in some countries compared to others, independent of the laboratory efficiency. This article revisits the basics of folliculogenesis involved in establishment of the antral follicle population and explores work in other species given the paucity of equine research in this area. The aim of the review is to elucidate interesting areas of further research that could generate essential information for clinicians and clients about the management and selection of the donor mare for OPU and potentially identify pharmacological targets for manipulation.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 2 ","pages":"e14625"},"PeriodicalIF":1.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0