Reproduction in Domestic Animals最新文献

Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In Vitro. 过表达 TAF4B 可促进卡特兰体外未分化精原细胞的增殖

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-11-01 DOI: 10.1111/rda.14738

Yuan Tian, Peng Zhang, Kemin Jing, Yuqian Li, Binglin Yue, Zhijuan Wu, Wenjing Dong, Jincheng Zhong, Xin Cai

{"title":"Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In Vitro.","authors":"Yuan Tian, Peng Zhang, Kemin Jing, Yuqian Li, Binglin Yue, Zhijuan Wu, Wenjing Dong, Jincheng Zhong, Xin Cai","doi":"10.1111/rda.14738","DOIUrl":"https://doi.org/10.1111/rda.14738","url":null,"abstract":"As the hybrid between cattle and yak, cattleyak is a typical male sterile mammal, and the underlying mechanism for its spermatogenic arrest is still unclear. In this study, the coding region of cattleyak TAF4B gene was cloned by RT-PCR and analysed by bioinformatics. To investigate the effects of TAF4B on cellular proliferation and differentiation, an expression vector was generated and introduced into undifferentiated spermatogonia (UDSPG) of cattleyak. The results showed that the protein encoded by TAF4B did not contain the signal peptide sequence. The expression level of TAF4B in UDSPG of cattleyak was lower than that in yak, while the overexpression of TAF4B in cattleyak promoted the proliferation activity of cattleyak UDSPG. Meanwhile, the expression of proliferation and meiosis-related genes was increased but the differentiation-related genes were decreased. Therefore, the aberrant expression of TAF4B in cattleyak UDSPG possibly impaired its proliferation and differentiation equilibrium and decreased its growth potentiality, thereby reducing the quantity of UDSPG and affecting spermatogenesis. This study provided a potential approach for further elucidation of the mechanism of spermatogenesis arrest and provided a new idea for solving the problem of male infertility in cattleyak.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementing the Beltsville Extender With Mitoquinol Improves the Quality and Fertility Potential of the Rooster's Cooled Sperm. 在贝尔茨维尔延长剂中添加线粒体喹诺酮可提高公鸡冷却精子的质量和生育能力。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-11-01 DOI: 10.1111/rda.14740

Reihaneh Nateghi, Reza Masoudi, Nader Asadzadeh

{"title":"Supplementing the Beltsville Extender With Mitoquinol Improves the Quality and Fertility Potential of the Rooster's Cooled Sperm.","authors":"Reihaneh Nateghi, Reza Masoudi, Nader Asadzadeh","doi":"10.1111/rda.14740","DOIUrl":"https://doi.org/10.1111/rda.14740","url":null,"abstract":"Supplementing freeze diluents with certain antioxidants can maintain the quality of chilled sperm. The present study was an attempt to investigate the effect of Beltsville extender supplementation with the mitochondrial-targeted antioxidant 'Mitoquinol' on the quality parameters and fertility potential of rooster sperm during the cooling process. Semen samples were diluted in Beltsville extender, divided into five groups, and supplemented with 0, 1, 10, 100 and 1000 nM Mitoquinol. Samples were stored at 5°C for up to 50 h and then assayed for sperm motility, viability, mitochondrial function, membrane integrity and malondialdehyde concentration after 0, 25 and 50 h of cooling. To assess reproductive performance, artificial insemination was performed using sperm cooled for 25 h. The results showed no differences between groups at the beginning time. Extender supplementation with 10 and 100 nM Mitoquinol resulted in an improvement in total motility, progressive motility, membrane integrity, mitochondrial function and viability (p ≤ 0.05), as well as a lower malondialdehyde concentration (p ≤ 0.05) in comparison to the other groups during 25 and 50 h storage. Fertility rates were higher when roosters were inseminated with semen samples supplemented with 10 and 100 nM Mitoquinol, compared to the control group. Therefore, supplementing Beltsville extender with Mitoquinol (10 and 100 nM) effective in improving the quality and fertility potential of cooled rooster sperm.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of silver nanoparticles on donkey sperm parameters and ultrastructure. 纳米银颗粒对驴精子参数和超微结构的影响

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14662

Verónica Pérez, Francisco Crespo, Angela I López, Soledad Cárdenas, María José Bautista, Manuel Hidalgo, Jesus Dorado, Isabel Ortiz

{"title":"Effect of silver nanoparticles on donkey sperm parameters and ultrastructure.","authors":"Verónica Pérez, Francisco Crespo, Angela I López, Soledad Cárdenas, María José Bautista, Manuel Hidalgo, Jesus Dorado, Isabel Ortiz","doi":"10.1111/rda.14662","DOIUrl":"https://doi.org/10.1111/rda.14662","url":null,"abstract":"The aim of this study was to determine the effect of silver nanoparticles (AgNPs) on donkey sperm parameters and ultrastructure. AgNPs were synthesized, purified and resuspended in the extender. Nine frozen-thawed donkey sperm samples were exposed to different concentrations of AgNPs (0, 1.25, 2.5, 5, 12.5, 25 and 50 μg/mL). Sperm parameters: total (TMOT, %) and progressive (PMOT, %) sperm motility, plasma (LIVE, %) and acrosomal membrane integrity (AIS, %), and sperm morphology (MORF, %) were evaluated immediately after AgNPs exposure (T0) and after 2 h of incubation (T2). The interaction beween AgNPs and spermatozoa was visualized by transmission electron microscopy (TEM). At T0, sperm motility and AIS were reduced (p < .05) when using concentrations ≥50 and ≥25 μg/mL, respectively. At T2, sperm motility and LIVE were significantly decreased (p < .05) in concentrations ≥25 and ≥50 μg/mL, respectively. TEM analysis revealed nanoparticle adhesion to the acrosomal region of the plasma membrane. In conclusion, AgNPs at concentrations ≥25 μg/mL impair motility, acrosome and plasma membrane integrity of donkey sperm, which may be mediated by adhesion to the acrosomal region of the sperm surface membrane.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of liquid extender, preservation temperature and time on the sheep sperm quality. 液体增稠剂、保存温度和时间对绵羊精子质量的影响

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14653

E Díaz-Hernández, P Bóveda, C M Picazo, J A Laborda-Gomariz, M Serralle, M Ramón, R Gallego, V Montoro, O García-Álvarez, R Fernández-Santos, J J Garde, A J Soler

引用次数: 0

Editorial. 社论

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14702

P L Lorenzo, J Santiago-Moreno

引用次数: 0

In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles. 猪精子与功能化超顺磁性纳米粒子的体外接触。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14654

Gabriela Garrappa, Cristina Martínez-López, María Jiménez-Movilla, Francisco A García-Vázquez

{"title":"In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles.","authors":"Gabriela Garrappa, Cristina Martínez-López, María Jiménez-Movilla, Francisco A García-Vázquez","doi":"10.1111/rda.14654","DOIUrl":"https://doi.org/10.1111/rda.14654","url":null,"abstract":"Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in vitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, although some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, in vitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Damage to Mitochondria During the Cryopreservation, Causing ROS Leakage, Leading to Oxidative Stress and Decreased Quality of Ram Sperm. 冷冻保存过程中线粒体受损，造成 ROS 泄漏，导致氧化应激和公羊精子质量下降。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14737

Liuming Zhang, Yuxuan Sun, Caiyu Jiang, Tariq Sohail, Xiaomei Sun, Jian Wang, Yongjun Li

{"title":"Damage to Mitochondria During the Cryopreservation, Causing ROS Leakage, Leading to Oxidative Stress and Decreased Quality of Ram Sperm.","authors":"Liuming Zhang, Yuxuan Sun, Caiyu Jiang, Tariq Sohail, Xiaomei Sun, Jian Wang, Yongjun Li","doi":"10.1111/rda.14737","DOIUrl":"10.1111/rda.14737","url":null,"abstract":"Semen cryopreservation can achieve long-term preservation of sperm. Ice crystal damage, as well as oxidative stress, result in mitochondrial dysfunction and a reduction in sperm motility after thawing. However, limited information exists regarding the impact of reactive oxygen species (ROS) and mitochondria on the cryopreservation of ram sperm. The primary objective of this study was to investigate the relationship between ROS and mitochondria concerning sperm quality during the cryopreservation of ram sperm. This investigation assessed sperm motility, kinematic characteristics, membrane integrity, acrosome integrity, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) levels, expression of mitochondrial respiratory genes (NDUFV2, SDHA, CYC1, and COXIV), ROS levels, malondialdehyde (MDA) content, phosphatidylserine externalisation rate, sperm ultrastructure, mtDNA copy number, expression of apoptosis-related genes (Bax, Caspase-3, and Caspase-8), Cytochrome C, and Caspase-3 content. The results showed the cryopreservation significantly (p < 0.05) decreased motility, kinetic parameters, membrane integrity, acrosome integrity, MMP, ATP, mRNA expression levels of mitochondrial respiratory-related genes, and significantly (p < 0.05) increased ROS levels, MDA content, phosphatidylserine externalisation rate, damage of sperm ultrastructure, mtDNA copy number, mRNA expression levels of apoptosis-related genes, Cytochrome C and Caspase-3 content compared to the fresh semen group. In conclusion, the cryopreservation causes damage to mitochondria, leading to increased ROS and subsequent oxidative stress. This process also initiates mitochondrial dysfunction and interferes with the electron transport chain, ultimately resulting in decreased MMP and ATP production. Furthermore, the liberation of Cytochrome C prompted the increase in Caspase-3 expression and subsequent sperm apoptosis occurred, ultimately leading to a deterioration in sperm quality after thawing.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lectin Functionalised Iron Magnetic Nanoparticle-Based Sperm Selection: A Potential Technique to Improve Bull Sperm Quality In Vitro. 基于凝集素功能化铁磁纳米粒子的精子选择：体外提高公牛精子质量的潜在技术。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14733

Nilendu Paul, A Kumaresan, T R Talluri, Kathan Raval, Kamaraj Elango, B S Pradeep Nag, Rajendran Duraisamy, A Manimaran

{"title":"Lectin Functionalised Iron Magnetic Nanoparticle-Based Sperm Selection: A Potential Technique to Improve Bull Sperm Quality In Vitro.","authors":"Nilendu Paul, A Kumaresan, T R Talluri, Kathan Raval, Kamaraj Elango, B S Pradeep Nag, Rajendran Duraisamy, A Manimaran","doi":"10.1111/rda.14733","DOIUrl":"https://doi.org/10.1111/rda.14733","url":null,"abstract":"Premature acrosomal exocytosis in cryopreserved semen is one of the reasons attributed to low fertility among livestock. In the present study, we attempted to enhance the cryopreserved semen quality by selective removal of acrosome-reacted spermatozoa using FITC-PNA conjugated iron magnetic nanoparticles (MNPs). Further, the effect of nano purification on other sperm functional attributes was also assessed. Iron MNPs were prepared using co-precipitation method and dextran-coated MNPs were conjugated with FITC-PNA (0.04 mg/mL). A preliminary experiment was conducted to standardise the dose of FITC-PNA conjugated iron MNPs (0.02, 0.05, 0.1, 0.15, 0.2, 0.4 and 0.6 mg). Among the different doses used, 0.6 mg FITC-PNA conjugated iron MNPs significantly (p < 0.05) removed higher acrosomal reacted spermatozoa from the semen, and therefore, this dose was used in further experiments. Cryopreserved semen from Holstein Friesian breeding bulls (n = 6) were thawed and washed using Sperm-TALP to remove residual extender. Washed spermatozoa (2 × 106) were exposed to 0.6 mg of FITC-PNA conjugated iron MNPs for 10 min at 37°C. The nano purified semen was assessed for various vital sperm parameters viz., viability, intracellular calcium, apoptosis, mitochondrial ROS and mitochondrial membrane potential using flow cytometry. We found that nanopurification using FITC-PNA conjugated iron MNPs significantly (p < 0.05) improved the sperm quality. The proportion of viable non-apoptotic spermatozoa with low intracellular calcium levels was significantly (p < 0.05) enriched in nano purified semen. Nano purification did not affect sperm mitochondrial membrane potential and ROS production. In conclusion, these preliminary findings indicate that FITC-PNA coated iron MNPs effectively removed acrosome reacted spermatozoa and significantly improved sperm functional attributes in the purified fraction.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolonged incubation of frozen-thawed equine spermatozoa for in vitro fertilization: A preliminary study using low temperature and INRA96 medium. 用于体外受精的冷冻解冻马精子的长时间培养：使用低温和 INRA96 培养基的初步研究。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14593

Marcos Luis-Calero, Carmen C Muñoz-García, Pablo Fernández-Hernández, Beatriz Macías-García, Lauro González-Fernández

{"title":"Prolonged incubation of frozen-thawed equine spermatozoa for in vitro fertilization: A preliminary study using low temperature and INRA96 medium.","authors":"Marcos Luis-Calero, Carmen C Muñoz-García, Pablo Fernández-Hernández, Beatriz Macías-García, Lauro González-Fernández","doi":"10.1111/rda.14593","DOIUrl":"https://doi.org/10.1111/rda.14593","url":null,"abstract":"A protocol for conventional in vitro fertilization (IVF) in horses using fresh semen has been described, using a prolonged incubation in FERT-TALP medium (22 h) at 38.2°C in the presence of penicillamine, hypotaurine and epinephrine (PHE). Our work aimed to develop a protocol that maintains quality parameters in frozen-thawed equine spermatozoa incubated for 22 h in the presence of PHE using different media (FERT-TALP and INRA96) and incubation temperatures (30 and 38.2°C). Twelve frozen ejaculates from four stallions were thawed and then incubated in either FERT-TALP or INRA96 with PHE at 30 or 38.2°C for 22 h. Following incubation, total motility (TM), progressive motility (PM), viability and acrosome integrity were evaluated. The results showed that TM was significantly higher (p < .001) at 30°C in both media, while PM was higher for INRA96 at 30°C compared to 38°C (p < .05). Moreover, INRA96 at 30°C exhibited higher sperm viability and acrosome integrity (p < .001) compared to the other experimental groups. These preliminary results suggest that incubating thawed equine spermatozoa at 30°C with PHE in INRA96 successfully maintains motility, viability and acrosome integrity in equine spermatozoa, indicating its potential use for conventional equine IVF.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes. 精液冷冻季节对青春期前山羊卵母细胞体外胚胎生产的影响。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14594

Mònica Ferrer-Roda, Jaime Catalán, Dolors Izquierdo, Maria Emilia Franco Oliveira, Maria-Teresa Paramio

{"title":"Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes.","authors":"Mònica Ferrer-Roda, Jaime Catalán, Dolors Izquierdo, Maria Emilia Franco Oliveira, Maria-Teresa Paramio","doi":"10.1111/rda.14594","DOIUrl":"https://doi.org/10.1111/rda.14594","url":null,"abstract":"Goat production is affected by reproductive seasonality. In vitro embryo production (IVEP) could overcome this effect. This study aimed to evaluate the impact of the season of semen collection/freezing on IVEP of prepubertal goat oocytes and on sperm quality and functionality concerning capacitation. Semen from six fertile bucks was collected, pooled and cryopreserved in spring and autumn and used for IVEP of oocytes recovered during the breeding season. Oocytes were IVM in TCM-199 with hormones, EGF and cysteamine; fertilized and cultured in BO-IVF and BO-IVC media (IVF Bioscience, UK). Semen samples were assessed at 0 and 3 h after culture in capacitating (BO-IVF, CAP) and non-capacitating conditions for sperm plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), intracellular calcium and plasma membrane lipid disorder. Blastocyst production was higher with spring sperm compared to autumn (12.0% vs. 2.1%, respectively; p < .05). After CAP, acrosome reaction and intracellular calcium were higher (p < .05) in spring than autumn sperm. No differences were found in other sperm parameters. In conclusion, seasonal variations in the IVEP of prepubertal goats could be linked to differences in sperm ability to undergo in vitro capacitation.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0