Reproduction in Domestic Animals最新文献

{"title":"Mangiferin Reduces Degeneration in Swine Preantral Follicles Enclosed in Ovarian Tissue Cultured In Vitro in the Presence of Doxorubicin.","authors":"Dax Ramyr E Silva Moreira, Gaby Judith Quispe Palomino, Isabor Sales Marinho Queiroz, Ana Flávia Bezerra da Silva, Anna Clara Accioly Ferreira, Benner Geraldo Alves, Selene Maia de Morais, Ariclécio Cunha de Oliveira, Ana Paula Ribeiro Rodrigues, Laritza Ferreira de Lima, José Ricardo de Figueiredo","doi":"10.1111/rda.70085","DOIUrl":"https://doi.org/10.1111/rda.70085","url":null,"abstract":"<p><p>This study assessed the potential protective effects of Mangiferin (MANGI) at various concentrations (10, 50 and 100 μM) on follicular survival and activation, enzyme activity of catalase (CAT) and superoxide dismutase (SOD), as well as the relative mRNA expression for BAX, BCL2 and SOD in porcine ovarian tissue cultured in vitro for 48 h with and without chemical stress induced by 0.3 μg/mL doxorubicin (DOX). The results indicated that when MANGI was associated with DOX, regardless of the tested concentration, MANGI increased (p < 0.05) follicle survival when compared to DOX alone, with only MANGI 10 + DOX and MANGI 50 + DOX treatments reducing follicular degeneration to levels comparable with the culture control. Regarding antioxidant enzymes, all treated groups decreased (p < 0.05) SOD activity compared to cultured control, while MANGI 10 treatment showed lower (p < 0.05) CAT activity compared to DOX treatment. Compared to cultured control, the mRNA levels of SOD were reduced (p < 0.05) in the MANGI 100, MANGI 10 + DOX and MANGI 100 + DOX treatments. In conclusion, supplementing the culture medium with MANGI attenuated the toxicity induced by DOX during the in vitro culture of pig preantral follicles enclosed in ovarian tissue.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70085"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Different Cryoprotectant Combinations in Testicular Vitrification in Dogs.","authors":"Jéssyka Araújo Noronha, Juliana de Souza Fernandes, Francisco Denilson Rodrigues Gomes, Julia Carrah Colares, Gisele Karla Sena Guimarães, Herlon Victor Rodrigues Silva, Lúcia Daniel Machado da Silva","doi":"10.1111/rda.70081","DOIUrl":"10.1111/rda.70081","url":null,"abstract":"<p><p>Testicular vitrification requires the use of high concentrations of cryoprotectants, which can cause damage to samples due to their toxicity. The combination of these substances comes up as a way to mitigate this problem. Thus, the aim of this study was to evaluate three cryoprotectant combinations in the testicular vitrification of dogs. Ten testicular pairs from adult dogs were used, from which 12 fragments of each pair were obtained, distributed among the fresh control group (CTR) and the experimental groups according to the cryoprotectant combinations tested: dimethyl sulfoxide (DMSO)/ethylene glycol (EG), DMSO/glycerol (GLY), and EG/GLY. The fragments were vitrified using the solid surface vitrification method (SSV), at a final concentration of 5.6 mol/L of the combined cryoprotectants. Subsequently, they were warmed up and processed for histomorphological morphometric evaluations and determination of mitochondrial activity with Rhodamine 123. Considering the morphological evaluation, the DMSO/EG group showed results similar to CTR, with good scores for nuclear integrity and cell organisation in the seminiferous tubules (p > 0.05). In contrast, the EG/GLY group presented greater nuclear condensation. It was difficult to visualise and distinguish between spermatogonia and Sertoli cells (p < 0.05). The DMSO/GLY group also showed distinct levels between spermatogonia and Sertoli cells, as well as nuclear condensation, which statistically differed from CTR (p < 0.05). Also, it was observed a random distribution of the remaining cells in the seminiferous tubules of the EG/GLY and DMSO/GLY groups. The three tested groups showed basement membrane retraction and a reduction of approximately 11.6% in the average diameter of the seminiferous tubules (p < 0.05). Vitrification did not influence the mitochondrial activity of the samples, regardless of the combination of cryoprotectants used (p > 0.05). It was concluded that the DMSO/EG combination best contributed to the maintenance of the testicular histomorphological structure of dogs after vitrification.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70081"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Negative Effects of Subclinical Pregnancy Toxaemia on Fetal Skeletal Muscle Development and Evaluation of the Protective Effects of Dietary l-Carnitine Supplementation in Sheep.","authors":"Ali Osman Turgut, Banu Kandil, Ebru Karakaya Bilen, Davut Koca, Ali Ünver, Fatma İşbilir, Özgür Özöner, Rahmi Doğan, Muhammed Furkan Önen, Mehmet Eroğlu, Sait Şendağ, Axel Wehrend","doi":"10.1111/rda.70088","DOIUrl":"10.1111/rda.70088","url":null,"abstract":"<p><p>This study evaluated the effects of subclinical pregnancy toxaemia (SPT) on fetal skeletal muscle development and assessed the potential protective role of L-carnitine supplementation during gestation. A total of 18 crossbred Hamdani ewes underwent oestrous synchronisation, natural mating and pregnancy confirmation via ultrasonography on day 45 post-mating. The ewes were managed according to NRC (2007) dietary guidelines until day 100 of gestation, after which they were assigned to three experimental groups: subclinical PT group (group 1; G1, n = 6), treatment group (subclinical PT + L-carnitine, group 2; G2, n = 6) and control group (group 3; G3, n = 6). Blood β-hydroxybutyrate (βHBA) concentrations were measured on day 100 and 138 of the gestation. Then, all ewes were slaughtered for fetal muscle sampling from the Musculus Longissimus Dorsi (MLD) and Vastus Lateralis (VL). Results indicated a significant reduction in muscle fibre number and fibre diameter in both MLD and VL in the SPT group (G1) compared to the control (G3) (p < 0.05). No significant differences were observed between G1 and G2 or between G2 and G3 for these parameters (p > 0.05). On the other hand, large effect sizes for group and pairwise comparisons imply that SPT may negatively affect prenatal muscle development and L-carnitine supports muscle development during the prepartum period. These findings highlight the negative effects of SPT and protective effects of L-carnitine supplementation on fetal skeletal muscle development in ewes with SPT. The observed deficits may negatively impact postnatal growth, survival rates and meat quality. Further investigations are warranted to optimise maternal nutrition strategies and evaluate therapeutic interventions aimed at mitigating the adverse impacts of SPT on fetal muscle development in ruminants. Furthermore, L-carnitine supplementation may be a useful in compensating for the negative effects of SPT.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70088"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biopsy of Porcine Blastocysts for Sex Identification and Transcriptome Sequencing Based on Laser-Assisted Microdissection.","authors":"Yan Liu, Ye Li, Wanchen Li, Mengru Ji, Yuting Zhang, Xinghong Ma, Zhonghua Liu, Xiaogang Weng","doi":"10.1111/rda.70087","DOIUrl":"https://doi.org/10.1111/rda.70087","url":null,"abstract":"<p><p>Pre-implantation embryo biopsy is a well-established technique in large livestock like cattle. However, its application in porcine embryos remains relatively limited. In this study, we successfully obtained trophectoderm cells from porcine IVP blastocysts using laser-assisted microdissection. First, we obtained trophectoderm cells of varying cell numbers (Group 1: less than three cells; Group 2: more than five cells) using laser-assisted microdissection and then evaluated the impact of this dissection procedure on embryo morphology. The morphological observation confirmed the complete normal post-biopsy recovery of blastocysts in the two groups. Furthermore, the expression of proteins associated with cell junction and structural integrity remained unaffected. Comparative analysis of blastocyst cell numbers and apoptotic rates among Group 1, Group 2 and the control group further validated the minimalinvasiveness of the laser-assisted microdissection when cutting less than three cells. The quality and utility of the biopsied trophectoderm cells were confirmed through successful sex identification and transcriptome sequencing. In conclusion, this study successfully established a minimal invasive laser-assisted microdissection technique for porcine blastocysts, enabling pre-implantation sex identification and blastocyst quality assessment.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70087"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Prevalence of Equine Coital Exanthema in a Thoroughbred Covering Station in Türkiye (2021-2024).","authors":"Yunus Emre Atay, Gencay Ekinci, Ali Erdem Öztürk, Mustafa Cem Timur, Alper Mete, Köksal Altınbay, Fatih Mehmet Derelli, Yaşar Akar, İhsan Keleş","doi":"10.1111/rda.70086","DOIUrl":"10.1111/rda.70086","url":null,"abstract":"<p><p>Equine Coital Exanthema (ECE) is an endemic herpesvirus disease primarily affecting the external genitalia and impairing mating activities in horses. Its extremely contagious nature, latency and subclinical features can result in outbreaks and significant economic losses. Transmission occurs primarily through mating activities; therefore, robust biosecurity measures are crucial in breeding facilities. This study aims to determine the clinical prevalence of ECE among horses in a covering station in Türkiye from 2021 to 2024. It also aims to assess the efficacy of routine PCR implementation within ECE's control strategies. A cross-sectional study design has been employed. Genital swab samples were collected from clinically suspected horses, which were tested for EHV-3 using real-time PCR. Animal records, clinical examination data and PCR test results were obtained from horses at the covering station between 2021 and 2024. During the 4 years (2021-2024), 9231 mating activities were carried out, and a total of 228 clinically suspected horses were tested for EHV-3 using real-time PCR. Among these 228 horses, 6 horses (2.6%) were confirmed positive for EHV-3. The primary weakness of this study is the failure to detect subclinical cases with PCR. The absence of follow-up PCR testing in two clinically infected horses represents a limitation of this study. The molecular diagnosis of ECE was reported for the first time in Türkiye. Clinical ECE cases infrequently transpired over the four-year period at the covering station. No outbreak transpired during this interval. PCR testing plays a crucial role in disease control when implemented with suitable management methods. Additional global epidemiological investigations on ECE are required.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70086"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine Congenital Defects Recorded in a National Survey of Dairy and Beef Herds Over Ten Years (2014-2023).","authors":"Katie Quigley, John F Mee","doi":"10.1111/rda.70067","DOIUrl":"https://doi.org/10.1111/rda.70067","url":null,"abstract":"<p><p>This study describes a novel model of farm-based congenital defect recording, with use of accessory data from a national breeding organisation. The study ran from 2014 to 2023 inclusive resulting in 522 reports of bovine congenital defects with additional data available for a subset (369/522). The most commonly reported defect was intestinal atresia (24.3%), followed by multiple defects (19.9%), and tail abnormalities (13.4%). This is the first national survey of bovine congenital defects in Ireland. It shows that while farmer-reported cases generally lack a veterinary clinical or necropsy examination, farmers are in the unique position of being able to report phenotypic abnormalities first-hand, and provide an unbiased perspective of the true typology and epidemiology of congenital defects on farm.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70067"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Signatures of Vulval Skin Surface: A Potential Non-Invasive Diagnostic Technology to Monitor the Calving Process in Water Buffalo (Bubalus bubalis).","authors":"Allu Teja, Jeyakumar Sakthivel, Kancharana Ananda Rao, Narayanan Krishnaswamy, Gowtham Varma Chintalapati, Vedamurthy Gowdar Veerappa, Arumugam Kumaresan, Kerekoppa P Ramesha, Muniandy Sivaram, Mukund A Kataktalware, Dayal N Das, Ravi Kumar Mula, Maharajan Lavanya","doi":"10.1111/rda.70065","DOIUrl":"https://doi.org/10.1111/rda.70065","url":null,"abstract":"<p><p>For the first time, the possibility of using infrared thermography to capture the temperature of the vulval skin surface around the calving process in order to forecast when the buffaloes' calving process will begin has been investigated. FLUKE infrared thermal camera was used to record the vulval skin surface temperature (VSST) and ocular temperature. Twenty-eight multiparous pregnant buffaloes had their thermograms taken at 6-h intervals between the 96th hour before the anticipated calving and the 24th hour after the calving. The eye and vulval skin surface showed a ΔT of 0.56°C and 0.45°C, respectively, indicating a considerable drop in temperature from 48 h before to calving. On the other hand, starting 6 h before calving, the vulval skin temperature showed an upward trend. There was minimal effect of circadian influence on the vulval skin surface temperature, as evidenced by the identical and significant declining pattern of the residual temperature of both the eye and VSST from 48 to 0 h of calving. Nonetheless, the reduction is ascribed to progesterone, which lowers vulval skin vascular perfusion and cutaneous circulation. Our study's findings suggested that, using related thermal signatures, vulval skin surface temperature variation in relation to calving as observed by digital infrared thermal imaging could be explored as a potential tool for predicting the onset of impending calving in buffaloes within -48 h.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70065"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance and Control of Colostrum Intake in the Neonate Pig, a Review.","authors":"P Langendijk","doi":"10.1111/rda.70077","DOIUrl":"10.1111/rda.70077","url":null,"abstract":"<p><p>This review discusses the biological significance of colostrum intake for neonatal development in pigs, and discusses the variation in colostrum intake in relation to piglet and sow factors. Lack of or insufficient colostrum intake reduces the odds of survival and compromises immunity, development of the gastrointestinal tract and other organs in neonate piglets. Colostrum intake is linearly related to pre-weaning gain. It is estimated that 10%-30% of piglets consume insufficient colostrum (< 250 g). Colostrum intake is less in piglets from larger litters, in piglets born later in the birth order, in piglets with low birth weight, and in piglets mildly or severely affected by asphyxia. Colostrum yield by sows peaks between parity two and five and then declines again, and is probably related to mammary gland tissue mass and endocrine factors involved in colostrogenesis. Before farrowing, the decline in progesterone facilitates and the increase in prolactin initiates secretion of colostrum, and the changes in both these hormones are related to colostrum yield. Feeding level and fibre may accelerate the decline in progesterone and in that way increase colostrum yield. Earlier during the third trimester of pregnancy, proper management of energy intake and sow body condition is important to optimise mammary gland development. Specific amino acids may be involved in mammary gland development, potentially through IGF-1.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70077"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Short-Term Timed Artificial Insemination Protocols With Estradiol Esters and PGF2α in Girolando Cows.","authors":"Mariana Dos Santos Dutra Okada, Lara Nogueira Silenciato, Samuel Rodrigues Bonamichi do Couto, Joaquim Esquerdo Ferreira, Marco Roberto Bourg de Mello","doi":"10.1111/rda.70076","DOIUrl":"10.1111/rda.70076","url":null,"abstract":"<p><p>Using a synchronisation protocol based on estradiol esters and prostaglandin, established in cattle since the 1970s, we implemented 24 or 48-h intervals between hormone administration and timed artificial insemination (TAI). This study compared two short-duration TAI protocols using estradiol benzoate (EB) and cypionate (EC) as ovulation inducers. A total of 172 Girolando female cows were selected after ovarian ultrasonographic evaluation and received 500 μg of prostaglandin F2α (PGF) at the start of the treatment (D0). The inclusion criterion for females in the study was the presence of a corpus luteum measuring ≥ 14 mm and at least one follicle with a diameter between 8 and 20 mm. The cows were randomly sorted into two treatment groups. The EB group received 2 mg of EB 24 h after PGF (D1), and TAI was performed 24 h later (D2). The EC group received 2 mg estradiol cypionate simultaneously with PGF, and TAI was performed 48 h later. Pregnancy was diagnosed 30 days after AI using transrectal ultrasonography. Experiment I evaluated follicular dynamics in 18 females (EB: n = 9; EC: n = 9) by analysing the follicular growth rate, pre-ovulatory follicle (POF) diameter, ovulation timing and ovulation rate. No statistically significant differences were observed (p > 0.05), with a 77.8% ovulation rate in both treatments. Experiment II compared the conception rates in 172 females (EB: n = 85; EC: n = 87). The overall conception rate was 30.2%, with no significant difference between the treatments (EB: 27.1%; EC: 33.3%). However, cows with a body condition score < 3.0 tended to have higher conception rates with EC than with EB (39.1% vs. 18.2%; p = 0.06). Cows with POF ≥ 10 mm at D0 had significantly higher conception rates with EC than with EB (48.3% vs. 29.0%; p = 0.03). Both protocols were equally effective; however, EC was more advantageous for cows with a POF ≥ 10 mm at treatment initiation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70076"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lectin-Histochemical Pattern on the Cystic and Neoplastic Ovaries of Bitches.","authors":"Juliano P Terra, Thiago B Almeida, Lucas P O Rezende, Claudio G Barbeito, Francisco Acuña, Guilherme R Blume, Rômulo S A Eloi, Letícia B Oliveira, André L R M Santos, Fabiano J F Sant'Ana","doi":"10.1111/rda.70075","DOIUrl":"10.1111/rda.70075","url":null,"abstract":"<p><p>Studies have demonstrated that many reproductive disorders can compromise the binding pattern of glycosidic residues, mainly in cows and sows. In the current study, the binding pattern of lectins was characterised and compared in normal and pathological ovaries of bitches. Fourteen biotinylated lectins (BSA-1B, Con-A, DBA, LCA, PHA-E, PHA-L, PNA, RCA-1, SBA, SJA, STA, WGA, s-WGA, UEA-1) were used. In ovaries without lesions, the predominant staining for RCA-1 was observed in the zona pellucida (ZP) of initial follicles, in the oocytes of primordial and primary follicles, and in the artery wall. For WGA, the reaction predominated in the mesothelium, in the ZP of secondary follicles, in the corpus luteum (CL) and in the oocyte of secondary follicles. Reactivity for STA was more pronounced in the mesothelium and ZP of developing follicles. The mesothelial cells and the CL were mainly marked for LCA. PHA-L was stained in the mesothelium, CL, and connective stroma, while the lectin PHA-E showed reactivity especially in the stroma, mesothelium, and artery wall. In the cystic ovaries, changes in the reaction of some lectins were noted in various structures adjacent to the cysts. RCA-1 staining was reduced in the ZP and oocyte of primordial, primary, and secondary follicles, while there was an increase of staining in endothelial cells. With WGA lectin, there was a reduction in CL and an increase in endothelial cells. Reactivity to STA was reduced in the ZP of primary and secondary follicles and increased in endothelial cells. LCA was also reduced in the CL and ZP of primary and secondary follicles. For the PHA-L lectin, the staining was reduced in the mesothelial cells, CL, and ZP of the primary follicles, while for PHA-E the reduction occurred in the stroma, oocyte of the primary follicles, and in the GC of the secondary follicles. In all neoplasms analysed (dysgerminoma, granulosa cell tumour, adenocarcinoma), there was also a reduction in reactivity to various lectins, both in the neoplastic cells compared to the cells of origin and in the adjacent non-neoplastic ovarian tissue. The data from the current study suggest that the changes in ovarian glycosidic residues observed in cysts and neoplasms may contribute to infertility in bitches.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70075"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}