Reproduction in Domestic Animals最新文献

Serum Ionised Calcium and Ionised Magnesium in Dairy Cows and Their Associations With the Incidence and Severity of Postpartum Metritis. 奶牛血清离子钙和离子镁及其与产后子宫炎发病率和严重程度的关系

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70057

Eduard Jose-Cunilleras, José Angel Robles-Guirado, José Ríos, Guillermo Elcoso, Alex Bach, Anna Bassols

{"title":"Serum Ionised Calcium and Ionised Magnesium in Dairy Cows and Their Associations With the Incidence and Severity of Postpartum Metritis.","authors":"Eduard Jose-Cunilleras, José Angel Robles-Guirado, José Ríos, Guillermo Elcoso, Alex Bach, Anna Bassols","doi":"10.1111/rda.70057","DOIUrl":"https://doi.org/10.1111/rda.70057","url":null,"abstract":"Peripartum is a critical period for dairy cows during which they may undergo postpartum diseases such as hypocalcaemia and metritis. Determination of serum total calcium (tCa) is widely used to monitor imbalances in this period, but determination of total magnesium (tMg) and ionised forms of both cations (iCa and iMg) has been less studied. In the present work, 42 healthy cows and 53 cows diagnosed with mild or moderate metritis were included. Serum samples were collected 10 days prepartum and 1, 3 and 10 days postpartum to determine tCa, iCa, tMg and iMg concentrations, as well as their corresponding ratios. Our results show that hypocalcaemia and hypermagnesaemia occur on Day 1 postpartum in cows that stayed healthy and cows undergoing metritis. Cows with moderate metritis showed lower serum concentrations of iCa and iMg throughout the period. Low values of prepartum iCa and iMg at 10 days before calving were associated with later development of metritis and therefore could represent potential prognostic indicators. In conclusion, the determination of iCa, tMg and iMg in serum, in addition to tCa, could offer a more comprehensive view of the ionic status of the periparturient dairy cow and may provide information about the risk of suffering uterine diseases.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70057"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Ultrasonic Evaluation of the Corpus Luteum in Alpacas With Embryo Removal on Day 9 Post-Mating".

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70056

引用次数: 0

Chlorogenic Acid Increases Follicle Survival, Stromal Cell Density, Levels of Thiol and Glutathione Peroxidase Activity in Cultured Bovine Ovarian Tissues.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70053

Pedro A A Barroso, Venância A N Azevedo, Danisvânia R Nascimento, Ernando I T de Assis, Miguel F De Lima Neto, Alesandro S Ferreira, Solano D Martins, Anderson W B Silva, Valdevane R Araújo, José R V Silva

{"title":"Chlorogenic Acid Increases Follicle Survival, Stromal Cell Density, Levels of Thiol and Glutathione Peroxidase Activity in Cultured Bovine Ovarian Tissues.","authors":"Pedro A A Barroso, Venância A N Azevedo, Danisvânia R Nascimento, Ernando I T de Assis, Miguel F De Lima Neto, Alesandro S Ferreira, Solano D Martins, Anderson W B Silva, Valdevane R Araújo, José R V Silva","doi":"10.1111/rda.70053","DOIUrl":"https://doi.org/10.1111/rda.70053","url":null,"abstract":"This study aimed to investigate the radical scavenging activity and the effects of chlorogenic acid on follicular survival and development, stromal cell density and collagen fibres distribution in extracellular matrix, as well as on mRNA expression of nuclear factor erythroid 2-related factor 2 (NRF2), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and periredoxin 6 (PRDX6), thiol levels, and activity of SOD, CAT, GPX enzymes in cultured bovine ovarian tissues. The free radical scavenging potential of chlorogenic acid was assessed through DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. The ovarian fragments were cultured in α-MEM+ alone or supplemented with 25, 50, 100, and 200 μmol/L chlorogenic acid for 6 days at 38.5°C and 5% CO2. Follicular growth and survival, stromal cell density, and collagen fibres were analysed by classical histology. Expression of mRNA for NRF2, SOD, CAT, GPX, and PRDX6 was evaluated by real-time PCR. Thiol levels and activity of SOD, CAT, GPX were investigated by Bradford method. The results showed that the presence of 100 μmol/L chlorogenic acid in culture medium promotes an increase in morphologically normal follicles and stromal cell density when compared to tissues cultured in control medium. Chlorogenic acid also increased thiol levels and GPX activity, but did not influence the distribution of collagen fibres or the expression of the analysed mRNAs. In conclusion,100 μmol/L chlorogenic acid increases the percentage of morphologically normal follicles, stromal cell density, thiol levels, and GPX activity in bovine ovarian tissues cultured in vitro.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70053"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HSP90 Is Required for Meiotic Resumption and Spindle Formation in Porcine Oocytes.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70060

Jae-Min Sim, Hyeon-Ji Song, Song-Hee Lee, Xiao-Han Li, Cheng-Lin Zhan, Qin-Yue Lu, Ji-Dam Kim, Gyu-Hyun Lee, Xiang-Shun Cui

{"title":"HSP90 Is Required for Meiotic Resumption and Spindle Formation in Porcine Oocytes.","authors":"Jae-Min Sim, Hyeon-Ji Song, Song-Hee Lee, Xiao-Han Li, Cheng-Lin Zhan, Qin-Yue Lu, Ji-Dam Kim, Gyu-Hyun Lee, Xiang-Shun Cui","doi":"10.1111/rda.70060","DOIUrl":"https://doi.org/10.1111/rda.70060","url":null,"abstract":"Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone that is expressed in response to stress conditions. HSP90 has been found to be involved in the activation of proteins related to cell division and female reproduction. However, its specific role in porcine oocyte maturation, particularly in cytoskeletal formation, remains unclear. In this study, geldanamycin (GA) was used to inhibit HSP90 activity by binding to its adenosine triphosphate (ATP) binding site. Porcine oocytes surrounded by cumulus cells were cultured in TCM-199 medium for 44 h, with varying concentrations of GA (0.1, 0.5, 1 and 2 μM). It was observed that oocyte maturation significantly decreased when treated with a concentration of 0.5 μM or higher, leading to an increase in oocytes arrested at the germinal vesicle and metaphase I (MI) stage. The expression levels of Cyclin-dependent kinase 1, p-Aurora C (Thr198), p-AKT (Ser473) and p-PLK1 (Thr210) decreased during the MI stage, whereas Polo-like kinase 1 remained consistent with the control group. Additionally, abnormal spindle formation was increased, with abnormalities including aberrant poles, misaligned chromosomes and failure to reach the proximity of the cell membrane. Moreover, examination of mature oocytes at the metaphase II (MII) stage revealed that GA treatment induced a decrease in BCL-2 phosphorylation at the Ser70 site and an increase at the Thr56 site. This led to the release of Cytochrome c from the mitochondria and upregulation of Caspase 3 expression. In conclusion, HSP90 is essential for proper meiotic maturation in porcine oocytes by playing critical roles in meiotic resumption and spindle formation.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70060"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA as Biomarkers for Physiological and Stress Processing in the Livestock.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70034

Montaser Elsayed Ali, Fatimah A Al-Saeed, Ahmed Ezzat Ahmed, Min Gao, Wei Wang, Haimiao Lv, Guohua Hua, Liguo Yang, Mohamed Abdelrahman

{"title":"MicroRNA as Biomarkers for Physiological and Stress Processing in the Livestock.","authors":"Montaser Elsayed Ali, Fatimah A Al-Saeed, Ahmed Ezzat Ahmed, Min Gao, Wei Wang, Haimiao Lv, Guohua Hua, Liguo Yang, Mohamed Abdelrahman","doi":"10.1111/rda.70034","DOIUrl":"https://doi.org/10.1111/rda.70034","url":null,"abstract":"Several microRNAs (miRNAs) have been identified as potential biomarkers widely dispersed in animals since 1993, and they have become a significant molecular biology research area. Because of their ability to activate extracellular molecules, stabilise bodily tissues, control cell-to-cell signals, and be easily extracted, miRNAs are outstandingly nominated as biomarkers. However, there is growing interest in targeting miRNAs to monitor physiological reproductive performance, including reproductive system development, embryo development, fertilisation, endocrinology, and animal welfare in stressful conditions. Moreover, miRNAs play significant roles in gene expression regulation; single miRNAs may have overlapping roles, and on a broader scale, multiple mRNAs govern a single function. Also, miRNAs serve as an intermediary messenger between the environment and reproductive performance, making them a vital component of miRNAs as performance biomarkers under environmental conditions like heat stress. This makes describing a unique miRNA's consequences and functions exceptionally challenging, which may confound many researchers. Also, enhancing our comprehension of miRNAs in response to testicular heat stress could potentially aid in preventing and treating spermatogenesis disorders. Therefore, the present review highlights miRNA's regulatory mechanisms on reproductive performance under heat stress to employ these findings in improving reproduction physiology research.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70034"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological Studies in the Endometrium of Fertile and Subfertile Bitches.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70055

Gail R McRae, Marco A Coutinho da Silva, Erin E Runcan, Julie A Stephens, Christopher Premanandan

{"title":"Histological Studies in the Endometrium of Fertile and Subfertile Bitches.","authors":"Gail R McRae, Marco A Coutinho da Silva, Erin E Runcan, Julie A Stephens, Christopher Premanandan","doi":"10.1111/rda.70055","DOIUrl":"10.1111/rda.70055","url":null,"abstract":"Endometrial biopsies have been used historically as a diagnostic tool as part of breeding management in some domestic species. In the mare, the findings in an endometrial biopsy can relate to the ability of that mare to carry a pregnancy to term. The value of the endometrial biopsy in the canine has not been fully established. In this study, the presence of inflammation, fibrosis and endometrial cysts was characterised in full-thickness uterine biopsies from subfertile bitches in late diestrus/early anestrus and compared to the same findings in fertile bitches. The hypothesis was that subfertile bitches would have more inflammation, fibrosis and endometrial cysts. Full-thickness uterine biopsies were taken at the hysterotomy site at the time of C-section for control cases (n = 103), and previous submissions were collected from The Ohio State Reproductive Service as subfertile samples (n = 263). The cases were blinded, randomised and evaluated. Chi-squared or Fisher's exact tests were used to compare categorical variables between control and experimental samples. The Wilcoxon rank-sum test was used to compare ages between the groups. Overall, inflammation was observed more frequently in the subfertile group in comparison to the control group. Lymphoplasmacytic inflammation was observed more frequently in the subfertile group when compared to the fertile group. The incidence of periglandular fibrosis and endometrial cysts was not significantly different between the two groups. These results indicate that microscopic evaluation of canine uterine biopsies may be more suited to detecting inflammatory changes in the endometrium and isolated endometrial cysts, as well as isolated fibrotic lesions, which may be less impactful on fertility compared to chronic inflammation.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70055"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fertility Traits in Japanese Black Cows in Farms Classified by Calving Intervals Using Epidemiological Methods.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-04-01 DOI: 10.1111/rda.70058

Osuke Suzuki, Mizuho Uematsu, Go Kitahara, Takeshi Osawa, Yosuke Sasaki

{"title":"Fertility Traits in Japanese Black Cows in Farms Classified by Calving Intervals Using Epidemiological Methods.","authors":"Osuke Suzuki, Mizuho Uematsu, Go Kitahara, Takeshi Osawa, Yosuke Sasaki","doi":"10.1111/rda.70058","DOIUrl":"10.1111/rda.70058","url":null,"abstract":"The calving interval (CI) is an important determinant of reproductive performance in Japanese Black cattle. The present study aimed to compare individual fertility traits among farms classified by CI. In total, 49,780 artificial insemination (AI) records for 7327 cows from 2011 to 2014 were obtained from 622 farms located in Miyazaki, Japan. The farms were classified into three groups (referred to as farm CI groups) using the lower and upper 25 percentiles of farm CI as thresholds. The mean CI of individual cows was 403.5 ± 70.9 days. The interquartile ranges of CI for individual cows were 49 days for short CI farms and 114 days for long CI farms. The interval between calving and the first AI was associated with farm CI group and parity (p < 0.05) but not with calving season and gestation length. The conception rate (CR) at the first AI in cows on long CI farms decreased as the interval from calving to the first AI decreased (p < 0.05). Additionally, no differences in CR among farm CI groups were observed for AI number ≥ 4. In long CI farms, cows with two AI treatments had longer interservice intervals than those for AI values of 3 and ≥ 4 (p < 0.05). In summary, fertility characteristics for long CI farms differed from those of farms with short or middle CI, and standard operating procedures need to be improved to decrease the CI.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70058"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Percentage of Normal Sperm Declines in Consecutively Collected Ejaculates of Ram Semen.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70028

Katherine R Seymour, Jessica P Rickard, Simon P de Graaf

{"title":"The Percentage of Normal Sperm Declines in Consecutively Collected Ejaculates of Ram Semen.","authors":"Katherine R Seymour, Jessica P Rickard, Simon P de Graaf","doi":"10.1111/rda.70028","DOIUrl":"10.1111/rda.70028","url":null,"abstract":"Sperm morphology is an important marker of fertility in livestock. Since poor sperm morphology can disqualify a sire from being sold or used for breeding, it is crucial to understand the factors that influence changes in sperm morphology over time. It is often hypothesised that little variation exists in sperm morphology from ejaculate to ejaculate so long as factors such as nutrition, season, health, and thermal status remain the same. This study aimed to test this hypothesis by investigating the morphology of sperm collected from rams (Ovine aries) (n = 3) twice daily, three times per week, over an 8-week period. Sperm morphology was evaluated using an eight-category classification system. Results revealed that each ram exhibited high levels of variability, with only 10.4% of ejaculates within ±5% of their own mean percentage normal sperm (PNS). The percentage of normal sperm over the 8-week period was significantly influenced by intra-week variation, although no consistent trend was observed across the full duration of the study. Within each day, a significant decline in the percentage of normal sperm was observed when a second ejaculate was collected consecutively. This decline was primarily attributed to an increase in cytoplasmic droplets and midpiece abnormalities. These findings suggest that rams subjected to regular semen collection in a controlled environment are likely to exhibit considerable variation in the percentage of normal sperm from ejaculate to ejaculate. For artificial breeding centres and veterinarians, repeated semen collection on the same day may reduce the proportion of normal sperm. As such, assessment of each separate ejaculate for sperm morphology is recommended before pooling for processing or insemination.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70028"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-Term Storage of Collared Peccary Semen in a Transport Container at 5°C Using Different Extenders.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70036

Romário Parente Dos Santos, Samara Sandy Jerônimo Moreira, Luana Grasiele Pereira Bezerra, Yuri Gonçalves Matos, Nayra Rachel Nascimento Luz, Pedro Augusto Pinheiro Brito, Pedro Nacib Jorge-Neto, Alexandre Rodrigues Silva

{"title":"Short-Term Storage of Collared Peccary Semen in a Transport Container at 5°C Using Different Extenders.","authors":"Romário Parente Dos Santos, Samara Sandy Jerônimo Moreira, Luana Grasiele Pereira Bezerra, Yuri Gonçalves Matos, Nayra Rachel Nascimento Luz, Pedro Augusto Pinheiro Brito, Pedro Nacib Jorge-Neto, Alexandre Rodrigues Silva","doi":"10.1111/rda.70036","DOIUrl":"https://doi.org/10.1111/rda.70036","url":null,"abstract":"The advancement of short-term storage methods for collared peccary semen targets its potential application in artificial insemination programmes and for combination with cryopreservation techniques. The objective of this study was to evaluate the performance of a transport container (Botutainer) for the preservation of collared peccary semen using commercial extenders (BTS, NUTRIXcell+ and PRIMXcell Ultra) as well as a TRIS + egg yolk extender. Ten ejaculates obtained by electroejaculation were diluted and stored at 5°C for 72 h. The cooled samples were analysed for kinetic aspects (using a computerised system), membrane functionality (hyposmotic test), membrane integrity and mitochondrial activity (fluorescent probes), morphology (Rose Bengal staining) and sperm binding capacity (to the perivitelline membrane of the egg yolk). After 72 h of storage, TRIS, NUTRIXcell and PRIMXcell preserved approximately 60% of motile sperm and 50%-65% of membrane integrity. In contrast, BTS was not effective in maintaining these parameters, preserving only ~40% and 50%, respectively (p < 0.05). All extenders preserved ~50% to 65% of mitochondrial activity and 72%-78% of normal sperm morphology up to 72 h. TRIS was the only extender that preserved ~75% of membrane functionality for 72 h. Finally, BTS exhibited a reduction in perivitelline membrane binding potential after 48 h (p < 0.05), whereas the other extenders showed results comparable to fresh semen. In summary, we demonstrate the effectiveness of the Botutainer device for preserving peccary semen at 5°C for up to 72 h using TRIS, NUTRIXcell and PRIMXcell extenders.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70036"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luteal Function and Pregnancy Rate in Bos taurus Heifers Treated With GnRH Prior to Timed Artificial Insemination.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70025

Julia Nobre Blank Camozzato, Bruna Favretto de Souza, João Marcello Rodrigues, Alinne Machado Petrarca Léo, Luiz Francisco Machado Pfeifer, Marcelo Bertolini, Monique Tomazele Rovani, Rafael Gianella Mondadori, Bernardo Garziera Gasperin, Fernando Caetano de Oliveira

{"title":"Luteal Function and Pregnancy Rate in Bos taurus Heifers Treated With GnRH Prior to Timed Artificial Insemination.","authors":"Julia Nobre Blank Camozzato, Bruna Favretto de Souza, João Marcello Rodrigues, Alinne Machado Petrarca Léo, Luiz Francisco Machado Pfeifer, Marcelo Bertolini, Monique Tomazele Rovani, Rafael Gianella Mondadori, Bernardo Garziera Gasperin, Fernando Caetano de Oliveira","doi":"10.1111/rda.70025","DOIUrl":"https://doi.org/10.1111/rda.70025","url":null,"abstract":"The study aimed to evaluate the effects of GnRH given 34 h after progesterone (P4) intravaginal device (IVD) removal in a timed artificial insemination (TAI) protocol. In Experiment 1, 17 Hereford and Braford heifers (control, n = 7; GnRH34; n = 10) received 2 mg estradiol benzoate (EB) and a 1 g P4 IVD on Day 0 (D0). On D8, IVDs were removed and 150 μg d-cloprostenol (PGF) and 1 mg estradiol cypionate (EC) were administered. In the GnRH34 group, animals received 25 μg lecirelin 34 h after the IVD removal (D9). TAI was performed in both groups on D10 (48 h after IVD removal). Follicular dynamics were evaluated from D8 until ovulation; blood samples were collected 7 and 12 days after TAI. In Experiment 2, to evaluate the effect of GnRH34 on pregnancy rates (control, n = 187; GnRH34; n = 203), heifers were subjected to the TAI protocols described in Experiment 1. No significant differences were observed in preovulatory follicle (POF) diameter on D10, follicular growth and ovulation rates until 72 h and P4 concentrations on days 7 and 12 after TAI (p > 0.05). Pregnancy rates (PR) were similar between groups (Control = 50.2%; GnRH34 = 50.2%; p = 0.65). A significant effect of body weight (BW) and body condition score (BCS) on PR was observed for control (p< 0.05), but not for GnRH34. In conclusion, GnRH 34 h after the IVD removal in beef heifers previously treated with EC had no effect on luteal function and fertility.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70025"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0