Reproduction in Domestic Animals最新文献

{"title":"Genomic Exploration of Selection Signatures Linked to Reproductive Traits in Locally Adapted Indicine, Taurine and Crossbred Cattle of India.","authors":"Sonali Sonejita Nayak, Divya Rajawat, Anurodh Sharma, Subhashree Parida, Triveni Dutt, Manjit Panigrahi","doi":"10.1111/rda.70093","DOIUrl":"https://doi.org/10.1111/rda.70093","url":null,"abstract":"<p><p>This study aims to comprehensively explore genome-wide selective processes influencing reproductive traits across six cattle breeds by employing different statistical methods. Reproductive efficiency is crucial for livestock productivity, as it directly influences the number of offspring and, consequently, the availability of animals for production. Early reproductive development and high fertility in herds boost selection intensity, driving faster genetic gains. This efficiency underpins the sustainability and profitability of livestock systems. To identify genomic signatures related to these traits, this study utilises genotyping technologies, including the Illumina BovineSNP50 Bead Chip and GGP Bos indicus 70k array. For this work, we used four summary statistics, including two intra-population statistics (Tajima's D and iHS), and two inter-population statistics (Rsb and XP-EHH). After identifying the key locations for selection, the NCBI database and the Cattle QTL database were utilised for annotation. The genes CACNA1H, KCNIP4, GDF9, SLC4A4, DHX57, EIF2AK3 and ME3 have been demonstrated to be under positive selection in Gir cattle. These are associated with characteristics such as udder cleft, age at puberty, sperm counts, sperm motility, sperm acrosome integrity rate, sperm motility per conception, sperm counts, conception rate, etc. Two genes, ENTHD1 and PRDM16 found on chromosomes 5 and 16, respectively, have been shared by Tharparkar and Gir which were undergoing positive selection. The ENTHD1 gene is linked to reproductive traits such as calving ease and stillbirth. Meanwhile, the PRDM16 gene is associated with characteristics like udder cleft, udder attachment, udder depth and udder height. The genes RXFP2, FRY, ENTHD1, SREBF2, RNF10, NYAP2, VWF, PPP1R8, EYA3, BBX, and TRPM3 were consistently identified across multiple selection signature methods, highlighting them as strong candidates under intense selection pressure. This approach offers valuable insights into the genetic basis of variations in reproductive traits, facilitating informed selection processes and enhancing our understanding of evolutionary and domestication in diverse cattle breeds.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 7","pages":"e70093"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct Warming of Vitrified In Vivo Equine Embryos.","authors":"Guilherme Couto, Agustina Grippo, Ann Ismer, Maarten Hoogewijs, Bussade Pedro, Lucas Vasconcelos, Gabriel Santos, Sandra Wilsher","doi":"10.1111/rda.70092","DOIUrl":"https://doi.org/10.1111/rda.70092","url":null,"abstract":"<p><p>Vitrified in vitro-produced embryos can be successfully warmed in isotonic media at room temperature (RT; 22°C). However, this protocol has not been reported for in vivo embryos, which are more challenging to vitrify and warm. Study objectives were to see if vitrified in vivo embryos warmed in RT isotonic medium gave equivalent pregnancy rates to stepwise serial dilution warming, and if embryo size influenced the results. One hundred and seventeen embryos were divided into groups by size (G1:≤ 300 μm, n = 59; G2:> 300-400 μm, n = 33; G3:> 400-500 μm, n = 24) and vitrified using a commercial vitrification kit. Embryos were placed in an equilibration solution for 10 (embryos ≤ 300 μm) or 15 min (embryos > 300 μm) before being moved to a vitrification solution (≤ 90 s) and loaded onto a Cryolock prior to plunging into LN2. Warming was undertaken by placing the tip of the uncapped Cryolock into RT isotonic medium (n = 77; comprised of G1:n = 45; G2:n = 22; G3:n = 10) or by stepwise serial dilution (n = 40) with the initial 1 M sucrose solution at 42°C (G1:n = 14; G2:n = 12; G3:n = 14). Warmed embryos were transferred to Day 6 recipient mares and pregnancy rates compared between warming protocols. Ignoring embryo size, there was no difference in Day 14 pregnancy rates for vitrified embryos warmed in RT isotonic medium versus stepwise serial dilution (70.1%, 54/77 vs. 82.5%, 33/40, respectively). No statistical difference existed in pregnancy rates between warming protocols for G1 or G2 embryos (p = 1.0, p = 0.439, respectively), but for G3 embryos, the stepwise protocol results in significantly more pregnancies (p < 0.001). The largest embryo successfully warmed in RT isotonic medium was 390 μm. Whereas for the stepwise protocol, the largest embryo was 480 μm. Direct warming in RT isotonic medium is a suitable protocol for warming vitrified embryos ≤ 390 μm, although the decline in pregnancies at the upper limit of G2 would suggest that clinically this methodology is suitable for embryos ≤ 360 μm.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 7","pages":"e70092"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Proceedings of the 26th European Veterinary Society for Small Animal Reproduction (EVSSAR) Congress, 3-5 July 2025, Porto, Portugal.","authors":"","doi":"10.1111/rda.70071","DOIUrl":"https://doi.org/10.1111/rda.70071","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 2 ","pages":"e70071"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Proceedings of the 26th European Veterinary Society for Small Animal Reproduction (EVSSAR) Congress, 3-5 July 2025, Porto, Portugal.","authors":"","doi":"10.1111/rda.70069","DOIUrl":"https://doi.org/10.1111/rda.70069","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 2 ","pages":"e70069"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Proceedings of the 26th European Veterinary Society for Small Animal Reproduction (EVSSAR) Congress, 3-5 July 2025, Porto, Portugal.","authors":"","doi":"10.1111/rda.70072","DOIUrl":"https://doi.org/10.1111/rda.70072","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 2 ","pages":"e70072"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Contralateral Pregnancies Are Carried to Term in Heifers but Not in Lactating Holstein Cows.","authors":"Tomas D Gonzalez, Sebastian G Umaña-Sedo, Segundo Casaro, Modesto E Hernandez, Rafael S Bisinotto, Pedro L J Monteiro, Klibs N Galvão","doi":"10.1111/rda.70090","DOIUrl":"https://doi.org/10.1111/rda.70090","url":null,"abstract":"<p><p>The following case report describes two pregnancies contralateral to the side of the corpus luteum (CL) that were carried to term after artificial insemination (AI) in Holstein heifers. Pregnancy diagnosis was performed by ultrasonography at 32, 46 and 74 days after timed AI. Both heifers had the pregnancy located within the right uterine horn and only one CL on the left ovary. Another eight lactating cows with contralateral pregnancies were followed around the same time; however, all lost the pregnancies between 32 days after AI and 74 days after AI. Pregnancies contralateral to the side of the CL have been described after embryo transfer in heifers, but to our knowledge, this is the first report of contralateral pregnancies after AI that were carried to term in heifers. In previous reports, all seven cows with contralateral pregnancies lost the pregnancies by 49 days of gestation. It is not clear why contralateral pregnancies can be successful in heifers but not in lactating Holstein cows. In summary, we confirmed that contralateral pregnancies were not viable in lactating Holstein cows but that successful contralateral pregnancies after AI are possible in Holstein heifers. The mechanism for this finding deserves further investigation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 7","pages":"e70090"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Proceedings of the 26th European Veterinary Society for Small Animal Reproduction (EVSSAR) Congress, 3-5 July 2025, Porto, Portugal.","authors":"","doi":"10.1111/rda.70070","DOIUrl":"https://doi.org/10.1111/rda.70070","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 2 ","pages":"e70070"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mangiferin Reduces Degeneration in Swine Preantral Follicles Enclosed in Ovarian Tissue Cultured In Vitro in the Presence of Doxorubicin.","authors":"Dax Ramyr E Silva Moreira, Gaby Judith Quispe Palomino, Isabor Sales Marinho Queiroz, Ana Flávia Bezerra da Silva, Anna Clara Accioly Ferreira, Benner Geraldo Alves, Selene Maia de Morais, Ariclécio Cunha de Oliveira, Ana Paula Ribeiro Rodrigues, Laritza Ferreira de Lima, José Ricardo de Figueiredo","doi":"10.1111/rda.70085","DOIUrl":"https://doi.org/10.1111/rda.70085","url":null,"abstract":"<p><p>This study assessed the potential protective effects of Mangiferin (MANGI) at various concentrations (10, 50 and 100 μM) on follicular survival and activation, enzyme activity of catalase (CAT) and superoxide dismutase (SOD), as well as the relative mRNA expression for BAX, BCL2 and SOD in porcine ovarian tissue cultured in vitro for 48 h with and without chemical stress induced by 0.3 μg/mL doxorubicin (DOX). The results indicated that when MANGI was associated with DOX, regardless of the tested concentration, MANGI increased (p < 0.05) follicle survival when compared to DOX alone, with only MANGI 10 + DOX and MANGI 50 + DOX treatments reducing follicular degeneration to levels comparable with the culture control. Regarding antioxidant enzymes, all treated groups decreased (p < 0.05) SOD activity compared to cultured control, while MANGI 10 treatment showed lower (p < 0.05) CAT activity compared to DOX treatment. Compared to cultured control, the mRNA levels of SOD were reduced (p < 0.05) in the MANGI 100, MANGI 10 + DOX and MANGI 100 + DOX treatments. In conclusion, supplementing the culture medium with MANGI attenuated the toxicity induced by DOX during the in vitro culture of pig preantral follicles enclosed in ovarian tissue.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70085"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Different Cryoprotectant Combinations in Testicular Vitrification in Dogs.","authors":"Jéssyka Araújo Noronha, Juliana de Souza Fernandes, Francisco Denilson Rodrigues Gomes, Julia Carrah Colares, Gisele Karla Sena Guimarães, Herlon Victor Rodrigues Silva, Lúcia Daniel Machado da Silva","doi":"10.1111/rda.70081","DOIUrl":"10.1111/rda.70081","url":null,"abstract":"<p><p>Testicular vitrification requires the use of high concentrations of cryoprotectants, which can cause damage to samples due to their toxicity. The combination of these substances comes up as a way to mitigate this problem. Thus, the aim of this study was to evaluate three cryoprotectant combinations in the testicular vitrification of dogs. Ten testicular pairs from adult dogs were used, from which 12 fragments of each pair were obtained, distributed among the fresh control group (CTR) and the experimental groups according to the cryoprotectant combinations tested: dimethyl sulfoxide (DMSO)/ethylene glycol (EG), DMSO/glycerol (GLY), and EG/GLY. The fragments were vitrified using the solid surface vitrification method (SSV), at a final concentration of 5.6 mol/L of the combined cryoprotectants. Subsequently, they were warmed up and processed for histomorphological morphometric evaluations and determination of mitochondrial activity with Rhodamine 123. Considering the morphological evaluation, the DMSO/EG group showed results similar to CTR, with good scores for nuclear integrity and cell organisation in the seminiferous tubules (p > 0.05). In contrast, the EG/GLY group presented greater nuclear condensation. It was difficult to visualise and distinguish between spermatogonia and Sertoli cells (p < 0.05). The DMSO/GLY group also showed distinct levels between spermatogonia and Sertoli cells, as well as nuclear condensation, which statistically differed from CTR (p < 0.05). Also, it was observed a random distribution of the remaining cells in the seminiferous tubules of the EG/GLY and DMSO/GLY groups. The three tested groups showed basement membrane retraction and a reduction of approximately 11.6% in the average diameter of the seminiferous tubules (p < 0.05). Vitrification did not influence the mitochondrial activity of the samples, regardless of the combination of cryoprotectants used (p > 0.05). It was concluded that the DMSO/EG combination best contributed to the maintenance of the testicular histomorphological structure of dogs after vitrification.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70081"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Negative Effects of Subclinical Pregnancy Toxaemia on Fetal Skeletal Muscle Development and Evaluation of the Protective Effects of Dietary l-Carnitine Supplementation in Sheep.","authors":"Ali Osman Turgut, Banu Kandil, Ebru Karakaya Bilen, Davut Koca, Ali Ünver, Fatma İşbilir, Özgür Özöner, Rahmi Doğan, Muhammed Furkan Önen, Mehmet Eroğlu, Sait Şendağ, Axel Wehrend","doi":"10.1111/rda.70088","DOIUrl":"10.1111/rda.70088","url":null,"abstract":"<p><p>This study evaluated the effects of subclinical pregnancy toxaemia (SPT) on fetal skeletal muscle development and assessed the potential protective role of L-carnitine supplementation during gestation. A total of 18 crossbred Hamdani ewes underwent oestrous synchronisation, natural mating and pregnancy confirmation via ultrasonography on day 45 post-mating. The ewes were managed according to NRC (2007) dietary guidelines until day 100 of gestation, after which they were assigned to three experimental groups: subclinical PT group (group 1; G1, n = 6), treatment group (subclinical PT + L-carnitine, group 2; G2, n = 6) and control group (group 3; G3, n = 6). Blood β-hydroxybutyrate (βHBA) concentrations were measured on day 100 and 138 of the gestation. Then, all ewes were slaughtered for fetal muscle sampling from the Musculus Longissimus Dorsi (MLD) and Vastus Lateralis (VL). Results indicated a significant reduction in muscle fibre number and fibre diameter in both MLD and VL in the SPT group (G1) compared to the control (G3) (p < 0.05). No significant differences were observed between G1 and G2 or between G2 and G3 for these parameters (p > 0.05). On the other hand, large effect sizes for group and pairwise comparisons imply that SPT may negatively affect prenatal muscle development and L-carnitine supports muscle development during the prepartum period. These findings highlight the negative effects of SPT and protective effects of L-carnitine supplementation on fetal skeletal muscle development in ewes with SPT. The observed deficits may negatively impact postnatal growth, survival rates and meat quality. Further investigations are warranted to optimise maternal nutrition strategies and evaluate therapeutic interventions aimed at mitigating the adverse impacts of SPT on fetal muscle development in ruminants. Furthermore, L-carnitine supplementation may be a useful in compensating for the negative effects of SPT.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70088"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}