Reproduction in Domestic Animals最新文献

{"title":"Overcoming Cryopreservation Challenges in Mammalian Sperm: Strategies for Mitigating Cryoinjuries.","authors":"Allai Larbi, Chunyan Li, Badaoui Bouabid, Guoquan Wu, Guobo Quan","doi":"10.1111/rda.70214","DOIUrl":"https://doi.org/10.1111/rda.70214","url":null,"abstract":"<p><p>Cryopreservation is a critical tool in the livestock industry, particularly for disseminating genetic materials and establishing sperm banks to preserve species. Developing a successful semen storage procedure is essential to minimize the negative effects caused by the cryopreservation process. Mammalian sperm are highly susceptible to cryoinjury and primarily inflicted during the freezing and thawing processes when extracellular water freezes and intracellular water is lost, negatively impacting sperm quality parameters and fertility. Cryoinjuries generally cause irreversible damage to sperm structure and function. Currently, various cryopreservation measures, such as selection of cryoprotectants and optimization of freezing and thawing processes, are employed to reduce sperm cryoinjuries and to elucidate the mechanisms underlying cryodamage. However, the exact nature of sperm cryoinjury remains largely unclear. This review discusses the current understanding of sperm cryodamage induced by the cryopreservation process, factors influencing sperm susceptibility to cryoinjury, the impacts of cryopreservation on proteins, metabolites, lipids, and RNAs. As one of the important measures to mitigate sperm cryoinjuries, the application of antioxidants is becoming increasingly important. So, the effects of antioxidants on post-thaw sperm quality are reviewed here.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70214"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Single Nucleotide Polymorphism Ss52050737 in Luteinizing Hormone/Choriogonadotropin Receptor Gene on Some Reproductive Traits in Friesian Cows.","authors":"Samar Samir Youssef, Eman Abd El-Razek Abbas, Ahmed S A Sosa, Mahmoud H Hasanain, Sally Ibrahim, Mohamed M M El-Sokary, Mahmoud F Nawito, Karima Gh M Mahmoud","doi":"10.1111/rda.70212","DOIUrl":"https://doi.org/10.1111/rda.70212","url":null,"abstract":"<p><p>The luteinizing hormone/choriogonadotropin receptor (LHCGR) gene is potentially a useful genetic marker for cattle reproductive status. This study aimed to investigate the impact of the LHCGR gene's ss52050737 polymorphism on age at first calving (AFC), calving interval (CI), and ovarian status using the TaqMan PCR assay. AFC was categorized into 25-34 and 35-45 months, while CI was grouped into 330-410 and 411-800 days. Ovarian status was classified as active or inactive ovaries. Blood samples were collected from cows using EDTA-containing vacutainer tubes and stored at -20°C until DNA extraction and analysis. Three genotypes (GG, GT, and TT) were identified for the LHCGR gene SNP (ss52050737). The homozygous GG genotype was more frequent in cows with a CI of 330-410 days, and the genotype distribution showed a statistically significant association (p = 0.018) in cows with CI 330-410 and 411-800 days. On the other hand, the GG genotype was not significantly different in relation to AFC and ovarian status (active or inactive). These findings suggest that the ss52050737 polymorphism of the LHCGR gene has an impact on calving interval, and the G allele is associated with the CI of 330-410 days.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70212"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laparoscopic Embryo Transfer in Swine: An Effective Alternative to Traditional Open Surgery.","authors":"H Baldassarre, L G Currin, M P de Macedo, V Guay, F L Facioli, R Duffy, A Ojerio, L Socransky, Z da Silva, K Gutierrez, W G Glanzner, V Bordignon","doi":"10.1111/rda.70220","DOIUrl":"10.1111/rda.70220","url":null,"abstract":"<p><p>While embryo transfer (ET) has not yet been widely adopted in the commercial swine industry, it is an essential tool in biomedical research, including the creation of gene-edited pigs. Traditionally, ET in pigs is performed by open surgery (laparotomy), an effective but invasive and time-consuming method associated with risks such as infection and herniation. To overcome these limitations, we developed a minimally invasive laparoscopic embryo transfer (LapET) method enabling ET into the oviduct using a sheep insemination device (Aspic, IMV Technologies, France). Seventeen gilts were synchronised with 17.6 mg altrenogest daily for 16 days, 10 mg dinoprost on D15, 1250 IU eCG on D16 and 1000 IU hCG on D19, followed by LapET on D21. Each gilt received an average of 160 embryos, transferred 24-48 h post-somatic cell nuclear transfer (SCNT). Under general anaesthesia, gilts were placed on a surgical table in a 30° incline Trendelenburg position. Three trocars were inserted to serve as ports for the laparoscope, a grasper and the Aspic pipette. The grasper served to expose the ovaries to confirm ovulation and to position the oviduct for embryo deposition. Following ET, the oviduct was released, instruments were removed and incisions were closed with surgical glue. Pregnancy was confirmed by ultrasonography, with 12 of 17 gilts (70.6%) diagnosed as pregnant, a success rate comparable to our recent laparotomy-based SCNT ETs into the oviduct (71.4%; 10 of 14). These results demonstrate that LapET is a viable, fast, minimally invasive and effective alternative to traditional open surgery ET in swine.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70220"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Protein Imbalance Impairs Sperm Morphology and Promotes Testicular Degeneration in Rams.","authors":"Edson Ramos de Siqueira Filho, Frederico Ozanam Papa, Pedro Henrique de Oliveira Baldini, Andrey Osvaldo Souza Ferro, Karina Alberti, Tiago Troncarellli, Gabriel Augusto Monteiro","doi":"10.1111/rda.70215","DOIUrl":"https://doi.org/10.1111/rda.70215","url":null,"abstract":"<p><p>Reproductive efficiency is a primary determinant of profitability in ruminant production, with male fertility being a critical factor for genetic progress and herd productivity. While nutritional management is a central component in modulating reproductive capacity, the effects of excessive dietary protein on testicular function remain incompletely elucidated. This study evaluated the impact of different dietary crude protein (CP) levels on reproductive and metabolic parameters in rams. Twenty-eight Santa Inês rams were randomly assigned to four groups (n = 7) and fed for 120 days with isoenergetic diets containing distinct CP levels: control (13.4%), hypoproteic (11.4%), hyperproteic I (17.5%) and hyperproteic II (22.4%). Periodic evaluations of body parameters, sperm kinetics and morphology, testicular ultrasonography, testicular cytology and hormonal profiles were conducted. Diets did not influence body weight or sperm motility (p > 0.05). However, variations in testicular echogenicity were observed (p < 0.05), and rams fed hyperproteic diets showed a progressive and marked increase in total sperm defects, exceeding 29.5% on day 80 (p < 0.05) compared with the control group (12.86%). Cytological analysis confirmed the presence of multinucleated giant cells and testicular degenerative changes from day 40 onwards in the hyperproteic groups. In conclusion, diets containing 17.5% and 22.4% CP are detrimental to sperm morphology and testicular integrity in rams, even in the absence of changes in body weight or detectable ultrasonographic alterations. The diet with 13.4% CP proved more suitable for maintaining testicular function and reproductive health.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70215"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-Encapsulated Vitamin E Enhances Cryo-Resistance of Stallion Spermatozoa: A Comprehensive Evaluation of Sperm Quality, Kinematic Parameters, Antioxidant Capacity, Microbiota and Ultrastructure.","authors":"Wael A Khalil, Hesham E Mostafa, Mohamed K Derbala, Mahmoud A E Hassan, Sameh A Abdelnour, Mostafa A El-Harairy","doi":"10.1111/rda.70213","DOIUrl":"https://doi.org/10.1111/rda.70213","url":null,"abstract":"<p><p>This experiment explores the comparative ability of vitamin E (VE) and its nano-emulsion (VEN) in enhancing cryo-resistance of stallion spermatozoa by examining semen quality, kinematic variables, acrosome integrity, apoptosis, antioxidant capacity, ultra-structural alterations and microbiota of cryopreserved stallion sperm. Semen from five stallions (8-10 years) was cryopreserved with 0.1 or 1 mM of VE or VEN; and a non-additive group served as the control. The VEN particles ranged in size from 72 to 172 nm, with a mean diameter of 287 nm. Supplementation with VE (1 mM) or VEN (0.1 or 1 mM) significantly enhanced progressive motility and viability during both the equilibration (for 30 min at 4°C) and post-thaw phases compared to the control (p < 0.05). Plasma membrane functionality was superior in all treated groups compared to control group (p < 0.001). Post-thaw sperm kinematic parameters and acrosome integrity were significantly enhanced in VEN treatment compared to VE and control groups. VEN (1 mM) notably improved antioxidant defences, specifically increasing TAC and catalase levels (p < 0.01) and a significant reduction in oxidative stress (NO, MDA and H₂O₂) in all treated semen samples (p < 0.01). VEN (1 mM) significantly reduced the proportion of apoptotic sperm (p < 0.01), increased viable sperm counts and the preservation of sperm ultrastructural integrity, as evidenced by intact acrosome and plasma membranes. VE and VEN treatments markedly lowered the total bacterial and coliform counts. Overall, supplementation with VEN (0.1 and 1 mM) effectively optimizes post-thaw stallion sperm quality by enhancing antioxidant defences and reducing the seminal microbiota.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 5","pages":"e70213"},"PeriodicalIF":1.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to 'Effects of the Deslorelin Slow-Release Implant (DSRI) on the Endocrine and Germinative Testicular Function in Tomcats'.","authors":"","doi":"10.1111/rda.70207","DOIUrl":"https://doi.org/10.1111/rda.70207","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 4","pages":"e70207"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Long-Term Changes in Endometrial Morphology and Functionality in Dairy Cows With Metritis.","authors":"M H Rashid, A Martelo Pereira, F N S Pereira, M C Oliveira Costa, E F Santos, L Christensen, J A Sexton, M Ciccarelli, L B Williams, M Binelli, M S Waqas, E B de Oliveira, C C Figueiredo","doi":"10.1111/rda.70206","DOIUrl":"10.1111/rda.70206","url":null,"abstract":"<p><p>This study evaluated the long-term impact of metritis on endometrial morphology and functionality in dairy cows. Postpartum Holstein cows (n = 107) were enrolled in a cohort study at the University of Idaho Dairy Center. Metritis was diagnosed using a Metricheck device within 12 days in milk (DIM) based on the presence of fetid, watery, reddish-brown vaginal discharge (VD). Cows diagnosed with metritis (MET; n = 9) were treated with ceftiofur crystalline free acid and paired with cows without metritis (NoMET; n = 9) of similar parity and DIM. Ovulation was synchronized using a Double Ovsynch protocol starting at 35 DIM, and ovulation was confirmed by ultrasonography. To eliminate confounding effects of semen or conceptus-derived signals, cows were not inseminated. Six days after ovulation (68-75 DIM), endometrial biopsy and cytology samples were collected. Endometrial morphology was assessed by histological evaluation of haematoxylin and eosin-stained sections. Endometrial transcriptomic profiles were determined by RNA sequencing from tissues collected via biopsy, and uterine fluid collected during cytology was analysed using metabolomic profiling. Differential gene expression was assessed using DESeq2, and metabolomic differences were evaluated using partial least squares discriminant analysis and PERMANOVA. Cows with metritis exhibited a numerically greater prevalence of periglandular fibrosis (63%; 95% Exact Binomial Confidence Interval [CI] = 25-92) compared with NoMET cows (44%; 95% CI = 14-79). Transcriptomic analysis identified 17 differentially expressed genes (FDR ≤ 0.10; |log2 fold change| > 1), including upregulation of genes associated with tissue repair in MET cows. No differences were detected in uterine fluid metabolomic profiles between groups. In conclusion, metritis was associated with persistent alterations in endometrial morphology and gene expression that were detectable months after clinical resolution, although these changes were not reflected in the uterine fluid metabolome.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 4","pages":"e70206"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Type of Extender and Freezing Method on Bovine Semen Post-Thawed Quality Characteristics and Bacterial Contamination.","authors":"Sara Ataei-Nazari, Michal Ďuračka, Filip Benko, Miroslava Kačániová, Peter Ivanič, Eva Tvrdá","doi":"10.1111/rda.70208","DOIUrl":"https://doi.org/10.1111/rda.70208","url":null,"abstract":"<p><p>Despite the considerable improvement in sperm cryopreservation regarding the procedures and cryoprotectants, the post-thawed semen quality characteristics still are far lower than the fresh semen sample. The current study aimed to investigate the efficacy of two semen extenders including ANDROMED (soy lecithin-based) and TRILADYL CSS (egg yolk-based) in combination with manual and automatic freezing programs on preserving post-thawed Bovine semen quality characteristics and bacterial contamination. The semen samples were cryopreserved via manual and automatic freezing using different extenders after passing the initial quality examination. Sperm quality characteristics and bacterial contamination were evaluated in fresh and post-thawed semen samples. The results demonstrated that regardless of the freezing program, the egg yolk-based extender is superior in preserving semen quality characteristics during cryopreservation; however, the post-thawed semen bacterial contamination was higher in semen samples extended in egg yolk-based extender (p < 0.0001). Moreover, the semen post-thawing quality characteristics were significantly higher in automatic compared to manual freezing (p < 0.0001). Regarding the interaction effects, the semen samples diluted with egg yolk-based extender and frozen via automatic freezing showed the highest post-thawing quality characteristics highlighting the higher suitability of egg yolk versus soy lecithin-based semen extender and also automatic versus manual freezing in preserving sperm quality characteristics during cryopreservation. The results of the current work demonstrate that using egg yolk-based extender in combination with automatic freezing can be acquired to preserve sperm quality during cryopreservation, whereas soy lecithin-based extender in combination with automatic freezing procedure can be used to control bacterial contamination if sperm quality characteristics are suitably qualified. However, research is needed to evaluate the competence of sperm cells from current treatments on oocyte fertility, conception, and pregnancy rates.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 4","pages":"e70208"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocrine Disruptive Effects of the Herbicides Linuron, Chloridazon and Metribuzin on Bovine Granulosa Cells.","authors":"Metehan Eser Kahveci, Ayse Arzu Yigit, Ruhi Kabakci","doi":"10.1111/rda.70204","DOIUrl":"10.1111/rda.70204","url":null,"abstract":"<p><p>Linuron, chloridazon and metribuzin are herbicides thought to have potential endocrine disruption (ED) effects. This study investigated the effects of linuron, chloridazon and metribuzin on bovine granulosa cell viability and oestrogen (E2) and progesterone (P4) secretion in vitro. Cells were exposed to 10, 50 and 250 μM linuron; 10, 100 and 1000 μM chloridazon, and 25, 250 and 2500 μM metribuzin for 48 and 96 h. At both time points, linuron had no effect on cell viability, whereas chloridazon and metribuzin decreased viability at the highest doses. E2 and P4 release decreased at 96 h in all groups, with significant differences observed at the highest doses (250 μM linuron, 1000 μM chloridazon and 2500 μM metribuzin). The study demonstrated that chloridazon and metribuzin exerted cytotoxic effects at the highest concentrations and that all three herbicides disrupted E2 and P4 secretion in a time-by-dose dependent manner. The results of this study may serve to raise awareness of the endocrine-disrupting effects of herbicides and to promote the consideration of alternatives for use that do not harm animal and human health.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 4","pages":"e70204"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13084294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Egg Yolk and Gum Arabic Extenders With Microencapsulation and α-Lipoic Acid Supplementation for Canine Semen Cryopreservation.","authors":"Shubham Mandhale, Mahesh Gupta, Jayant Pandurang Korde, Krushna Bibichand Bahiram, Sachin Wasudeorao Bonde, Manoj Patil, Rohit Rathod, Pratik Kokate, Nitin Eknath Gade","doi":"10.1111/rda.70210","DOIUrl":"https://doi.org/10.1111/rda.70210","url":null,"abstract":"<p><p>Cryopreservation is an essential component of assisted reproductive technologies in dogs. However, post-thaw semen quality is often compromised by cryo-induced oxidative stress, membrane disruption, and DNA damage, which ultimately reduce fertility outcomes. Conventional egg yolk (EY)-based extenders provide partial protection during freezing but pose biosecurity rick and exhibit batch-to-batch variability. Gum arabic (GA), a plant-derived polysaccharide with cryoprotective and antioxidant properties, has emerged as a potential alternative. The present study evaluated the comparative efficacy of EY and GA extenders, with and without microencapsulation and α-lipoic acid (ALA) supplementation, on post-thaw semen quality in dogs. Semen was collected from six sexually mature Labrador retrievers, with three ejaculates obtained from each dog. Semen samples were cryopreserved in straws under three treatment conditions: extender alone (Treatment 1), extender with microencapsulation (Treatment 2), and extender with microencapsulation plus ALA (Treatment 3). Post-thaw evaluation included sperm motility, viability, acrosomal integrity, hypo-osmotic swelling test (HOST), DNA fragmentation index (DFI), mitochondrial membrane potential (MMP), and total antioxidant capacity (TAC). The results indicated that microencapsulation and ALA supplementation improved several post-thaw sperm quality parameters. Treatment 3 showed the highest values of sperm viability, plasma membrane integrity, acrosomal integrity, MMP, and total antioxidant capacity in both extenders. Sperm motility and DFI did not differ significantly among treatments, although numerically lower DFI values were observed in GA-based treatments. In conclusion, the combined use of GA extender, sperm microencapsulation, and ALA supplementation may enhance the preservation of canine sperm functional integrity during cryopreservation. Further studies evaluating fertility outcomes are required to confirm the practical applicability of these approaches in canine reproductive technologies.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 4","pages":"e70210"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}