Reproduction in Domestic Animals最新文献

{"title":"Bovine Congenital Defects Recorded in a National Survey of Dairy and Beef Herds Over Ten Years (2014-2023).","authors":"Katie Quigley, John F Mee","doi":"10.1111/rda.70067","DOIUrl":"https://doi.org/10.1111/rda.70067","url":null,"abstract":"<p><p>This study describes a novel model of farm-based congenital defect recording, with use of accessory data from a national breeding organisation. The study ran from 2014 to 2023 inclusive resulting in 522 reports of bovine congenital defects with additional data available for a subset (369/522). The most commonly reported defect was intestinal atresia (24.3%), followed by multiple defects (19.9%), and tail abnormalities (13.4%). This is the first national survey of bovine congenital defects in Ireland. It shows that while farmer-reported cases generally lack a veterinary clinical or necropsy examination, farmers are in the unique position of being able to report phenotypic abnormalities first-hand, and provide an unbiased perspective of the true typology and epidemiology of congenital defects on farm.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70067"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Signatures of Vulval Skin Surface: A Potential Non-Invasive Diagnostic Technology to Monitor the Calving Process in Water Buffalo (Bubalus bubalis).","authors":"Allu Teja, Jeyakumar Sakthivel, Kancharana Ananda Rao, Narayanan Krishnaswamy, Gowtham Varma Chintalapati, Vedamurthy Gowdar Veerappa, Arumugam Kumaresan, Kerekoppa P Ramesha, Muniandy Sivaram, Mukund A Kataktalware, Dayal N Das, Ravi Kumar Mula, Maharajan Lavanya","doi":"10.1111/rda.70065","DOIUrl":"https://doi.org/10.1111/rda.70065","url":null,"abstract":"<p><p>For the first time, the possibility of using infrared thermography to capture the temperature of the vulval skin surface around the calving process in order to forecast when the buffaloes' calving process will begin has been investigated. FLUKE infrared thermal camera was used to record the vulval skin surface temperature (VSST) and ocular temperature. Twenty-eight multiparous pregnant buffaloes had their thermograms taken at 6-h intervals between the 96th hour before the anticipated calving and the 24th hour after the calving. The eye and vulval skin surface showed a ΔT of 0.56°C and 0.45°C, respectively, indicating a considerable drop in temperature from 48 h before to calving. On the other hand, starting 6 h before calving, the vulval skin temperature showed an upward trend. There was minimal effect of circadian influence on the vulval skin surface temperature, as evidenced by the identical and significant declining pattern of the residual temperature of both the eye and VSST from 48 to 0 h of calving. Nonetheless, the reduction is ascribed to progesterone, which lowers vulval skin vascular perfusion and cutaneous circulation. Our study's findings suggested that, using related thermal signatures, vulval skin surface temperature variation in relation to calving as observed by digital infrared thermal imaging could be explored as a potential tool for predicting the onset of impending calving in buffaloes within -48 h.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70065"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Short-Term Timed Artificial Insemination Protocols With Estradiol Esters and PGF2α in Girolando Cows.","authors":"Mariana Dos Santos Dutra Okada, Lara Nogueira Silenciato, Samuel Rodrigues Bonamichi do Couto, Joaquim Esquerdo Ferreira, Marco Roberto Bourg de Mello","doi":"10.1111/rda.70076","DOIUrl":"10.1111/rda.70076","url":null,"abstract":"<p><p>Using a synchronisation protocol based on estradiol esters and prostaglandin, established in cattle since the 1970s, we implemented 24 or 48-h intervals between hormone administration and timed artificial insemination (TAI). This study compared two short-duration TAI protocols using estradiol benzoate (EB) and cypionate (EC) as ovulation inducers. A total of 172 Girolando female cows were selected after ovarian ultrasonographic evaluation and received 500 μg of prostaglandin F2α (PGF) at the start of the treatment (D0). The inclusion criterion for females in the study was the presence of a corpus luteum measuring ≥ 14 mm and at least one follicle with a diameter between 8 and 20 mm. The cows were randomly sorted into two treatment groups. The EB group received 2 mg of EB 24 h after PGF (D1), and TAI was performed 24 h later (D2). The EC group received 2 mg estradiol cypionate simultaneously with PGF, and TAI was performed 48 h later. Pregnancy was diagnosed 30 days after AI using transrectal ultrasonography. Experiment I evaluated follicular dynamics in 18 females (EB: n = 9; EC: n = 9) by analysing the follicular growth rate, pre-ovulatory follicle (POF) diameter, ovulation timing and ovulation rate. No statistically significant differences were observed (p > 0.05), with a 77.8% ovulation rate in both treatments. Experiment II compared the conception rates in 172 females (EB: n = 85; EC: n = 87). The overall conception rate was 30.2%, with no significant difference between the treatments (EB: 27.1%; EC: 33.3%). However, cows with a body condition score < 3.0 tended to have higher conception rates with EC than with EB (39.1% vs. 18.2%; p = 0.06). Cows with POF ≥ 10 mm at D0 had significantly higher conception rates with EC than with EB (48.3% vs. 29.0%; p = 0.03). Both protocols were equally effective; however, EC was more advantageous for cows with a POF ≥ 10 mm at treatment initiation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70076"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lectin-Histochemical Pattern on the Cystic and Neoplastic Ovaries of Bitches.","authors":"Juliano P Terra, Thiago B Almeida, Lucas P O Rezende, Claudio G Barbeito, Francisco Acuña, Guilherme R Blume, Rômulo S A Eloi, Letícia B Oliveira, André L R M Santos, Fabiano J F Sant'Ana","doi":"10.1111/rda.70075","DOIUrl":"10.1111/rda.70075","url":null,"abstract":"<p><p>Studies have demonstrated that many reproductive disorders can compromise the binding pattern of glycosidic residues, mainly in cows and sows. In the current study, the binding pattern of lectins was characterised and compared in normal and pathological ovaries of bitches. Fourteen biotinylated lectins (BSA-1B, Con-A, DBA, LCA, PHA-E, PHA-L, PNA, RCA-1, SBA, SJA, STA, WGA, s-WGA, UEA-1) were used. In ovaries without lesions, the predominant staining for RCA-1 was observed in the zona pellucida (ZP) of initial follicles, in the oocytes of primordial and primary follicles, and in the artery wall. For WGA, the reaction predominated in the mesothelium, in the ZP of secondary follicles, in the corpus luteum (CL) and in the oocyte of secondary follicles. Reactivity for STA was more pronounced in the mesothelium and ZP of developing follicles. The mesothelial cells and the CL were mainly marked for LCA. PHA-L was stained in the mesothelium, CL, and connective stroma, while the lectin PHA-E showed reactivity especially in the stroma, mesothelium, and artery wall. In the cystic ovaries, changes in the reaction of some lectins were noted in various structures adjacent to the cysts. RCA-1 staining was reduced in the ZP and oocyte of primordial, primary, and secondary follicles, while there was an increase of staining in endothelial cells. With WGA lectin, there was a reduction in CL and an increase in endothelial cells. Reactivity to STA was reduced in the ZP of primary and secondary follicles and increased in endothelial cells. LCA was also reduced in the CL and ZP of primary and secondary follicles. For the PHA-L lectin, the staining was reduced in the mesothelial cells, CL, and ZP of the primary follicles, while for PHA-E the reduction occurred in the stroma, oocyte of the primary follicles, and in the GC of the secondary follicles. In all neoplasms analysed (dysgerminoma, granulosa cell tumour, adenocarcinoma), there was also a reduction in reactivity to various lectins, both in the neoplastic cells compared to the cells of origin and in the adjacent non-neoplastic ovarian tissue. The data from the current study suggest that the changes in ovarian glycosidic residues observed in cysts and neoplasms may contribute to infertility in bitches.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70075"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Ram Semen Quality After Supplementation With Gelatin, Agar or Alginate Prior to Cooling Storage\".","authors":"","doi":"10.1111/rda.70063","DOIUrl":"https://doi.org/10.1111/rda.70063","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70063"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Oestrus and Hormonal Changes in the Progeny of 2 Genotypes of FecB Gene-Edited Sheep.","authors":"Yang Chulei, Zhang Yiyuan, Tang Hong, Guo Yanhua, Wang Limin, Zhou Ping","doi":"10.1111/rda.70038","DOIUrl":"https://doi.org/10.1111/rda.70038","url":null,"abstract":"<p><p>The FecB gene, the first gene identified for multiple births, can improve reproductive performance in sheep. We used the CRISPR/Cas9 editing system to obtain the FecB gene in Kazakh sheep and mated with unedited Kazakh sheep. After the genotypic screening of the progeny, four B+ and ++ Kazakh ewes, each 2 weeks old and healthy, and close to the same body weight, were selected to study oestrus and hormonal changes in the progeny population of FecB gene-edited Kazakh sheep. Our results showed that the FecB gene was moderately polymorphic (0.25 < PIC < 0.5) in the progeny population, was able to be stably inherited, and showed increased lambing numbers. The number of follicles and tiny follicles differed significantly (p < 0.05) between the 2 genotypes of ewes in the progeny population. Physiologically, FecB gene-edited sheep did not differ from wild-type sheep. Throughout the oestrous cycle, FSH, LH, and E₂ in serum hormones of both sheep genotypes showed variability (p < 0.05) between 72-360 h, 360-528 h, and 12-72 h, respectively, compared to other periods. The concentration of FSH and PROG in follicular fluid was much higher than that of LH and E₂ (p < 0.05). Overall, it is possible to make Kazakh sheep carry the FecB gene by CRISPR/Cas9 editing and stably inherit it into the progeny population. The enhancement of reproductive hormones by the FecB gene is beneficial to follicular development and ovulation and improves the reproductive efficiency of sheep.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70038"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Improves the Development Ability of Sheep Embryos by Reducing ROS During IVEP.","authors":"Hang Zhang, Smhaa Gahallah, Yucheng Liu, Jingjing Wang, Zhenliang Zhang, Xueming Zhao, Pengcheng Wan","doi":"10.1111/rda.70061","DOIUrl":"https://doi.org/10.1111/rda.70061","url":null,"abstract":"<p><p>Sheep in vitro embryo production (IVEP) is of great economic and scientific value, while reactive oxygen species (ROS) can negatively impact the quality and developmental potential of sheep embryos. Therefore, reducing ROS may enhance the efficacy of sheep IVEP. In this study, different concentrations of melatonin (10^-9, 10^-7, 10^-5, 10^-3 M) were supplemented during oocyte in vitro maturation (IVM) and embryo in vitro culture (IVC). The developmental ability of sheep oocytes and embryos, the survival rate of vitrified blastocysts, ROS level, ATP content, ATP synthase activity and related gene expression levels of sheep blastocysts were detected. The results showed that 10^-7 M melatonin significantly improved the developmental ability of sheep oocytes and embryos, elevated the survival rate of blastocysts after vitrification, reduced the ROS level, increased the ATP content and ATP synthase activity and improved the expression levels of quality-related genes in sheep blastocysts. We speculated that melatonin may enhance the developmental competence of sheep oocytes and embryos by reducing the ROS level and promoting ATP synthesis. In addition, high concentrations of melatonin may have an adverse impact on the development of sheep embryos.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70061"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Three Combinations of Cryoprotectants and Two Warming Temperatures on Cellular Morphology, Morphometry and Mitochondrial Activity of Vitrified Canine Testicles.","authors":"Juliana de Souza Fernandes, Jéssyka Araújo Noronha, Francisco Denilson Rodrigues Gomes, Bruna Farias Brito, Gisele Karla Sena Guimarães, Herlon Victor Rodrigues Silva, Leda Maria Costa Pereira Bersano, Lúcia Daniel Machado da Silva","doi":"10.1111/rda.70074","DOIUrl":"10.1111/rda.70074","url":null,"abstract":"<p><p>High concentrations of cryoprotectants required for testicular vitrification result in a toxic environment for cells. To mitigate this issue, a suitable alternative is to combine cryoprotectants. The temperature for warming a vitrified sample is also important to assure cell viability. Thus, the aim of this work was to evaluate how combining cryoprotectants (ethylene glycol-EG, glycerol-GLY, and dimethyl sulfoxide-DMSO) in pairs and using two warming temperatures (37°C and 50°C) influence cellular morphology, tubular morphometry, and mitochondrial activity after testicular vitrification of dogs. Testicular fragments from ten adult dogs were distributed among the fresh control group (CTR) and the experimental groups according to the combination of cryoprotectants and temperatures (EG/GLY37, EG/GLY50, DMSO/GLY37, DMSO/GLY50, DMSO/EG37 and DMSO/EG50). The fragments were vitrified in a final concentration of 5.6 mol/L (2.8 mol/L of each of the cryoprotectants combined two by two) and subsequently warmed up to 37°C/30 s or 50°C/5 s. Following this, they were processed for histomorphological, morphometric, and mitochondrial activity evaluations with Rhodamine 123. In the morphometric evaluation, all vitrified groups showed a significant reduction in tubular diameter (p < 0.05). All experimental groups showed greater basement membrane separation when related to the CTR (p < 0.05). DMSO/EG37 showed the greatest basement membrane separation when compared to all other groups (p < 0.05). Regarding membrane retraction, all vitrified groups, regardless of the warming temperature, had greater retraction when related to CTR (p < 0.05), except DMSO/GLY50, which did not differ from any group (p > 0.05). Regarding the distinction between spermatogonia and Sertoli cells, no groups warmed up to 50°C differed from the control, except DMSO/GLY37. For nuclear visualisation, none of the vitrified groups differed from the CTR (p > 0.05), except DMSO/GLY37 (p < 0.05), which showed better nuclear visualisation. For the nuclear condensation parameter, there were no significant differences among the groups (p > 0.05). Mitochondrial activity was reduced in all vitrified samples, regardless of the combination of cryoprotectants and warming temperature (p > 0.05). It was concluded that the association of DMSO/GLY50 presented better preservation of morphological aspects.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70074"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Ionised Calcium and Ionised Magnesium in Dairy Cows and Their Associations With the Incidence and Severity of Postpartum Metritis.","authors":"Eduard Jose-Cunilleras, José Angel Robles-Guirado, José Ríos, Guillermo Elcoso, Alex Bach, Anna Bassols","doi":"10.1111/rda.70057","DOIUrl":"10.1111/rda.70057","url":null,"abstract":"<p><p>Peripartum is a critical period for dairy cows during which they may undergo postpartum diseases such as hypocalcaemia and metritis. Determination of serum total calcium (tCa) is widely used to monitor imbalances in this period, but determination of total magnesium (tMg) and ionised forms of both cations (iCa and iMg) has been less studied. In the present work, 42 healthy cows and 53 cows diagnosed with mild or moderate metritis were included. Serum samples were collected 10 days prepartum and 1, 3 and 10 days postpartum to determine tCa, iCa, tMg and iMg concentrations, as well as their corresponding ratios. Our results show that hypocalcaemia and hypermagnesaemia occur on Day 1 postpartum in cows that stayed healthy and cows undergoing metritis. Cows with moderate metritis showed lower serum concentrations of iCa and iMg throughout the period. Low values of prepartum iCa and iMg at 10 days before calving were associated with later development of metritis and therefore could represent potential prognostic indicators. In conclusion, the determination of iCa, tMg and iMg in serum, in addition to tCa, could offer a more comprehensive view of the ionic status of the periparturient dairy cow and may provide information about the risk of suffering uterine diseases.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70057"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Gestational Age in Warmblood, Standardbred and Thoroughbred Horses Based on the Table Established in Light Breed Horses Using Foetal Ultrasonographic Measurements of Four Biometric Parameters.","authors":"Catherine D Renaudin, Valerie Picandet, Philip H Kass","doi":"10.1111/rda.70059","DOIUrl":"https://doi.org/10.1111/rda.70059","url":null,"abstract":"<p><p>Using the equation of Hartwig et al. and the table established for light breed horses, day of gestation was predicted in 108 Warmblood (WB) and 59 racehorses (Thoroughbred [TB] and Standardbred [STB]) pregnant mares. In WB, gestational ages were predicted within 2 weeks from 100 to 200 days of gestation using biparietal diameter (BPD), aortic diameter (AortD) or femur length (FL); within 2 weeks from 100 to 250 days using BPD or AortD; within 2 weeks from 100 to 300 days using BPD alone; and within 3 weeks from 100 to 350 days using eye approximated volume (EyV) alone. In the racehorse group, gestational ages were predicted within 2 weeks from 100 to 200 days of gestation using BPD or AortD; within 3 weeks from 100 to 250 days of gestation using BPD or FL; and within 3 weeks after 300 days of gestation using EyV. Across breeds including Quarter Horses (QH), WB, STB and TB, BPD was the best foetal age predictor from 100 to 200 days of gestation and from 200 to 300 days of gestation with accuracies within 2 and 3 weeks, respectively. After 300 days of gestation, EyV was found to be the best predictor with an accuracy of within 3 weeks. Overall, in the two groups, the breed of the foal nor the breed of the dam (except for short days increments), had any effect on foetal age prediction. Therefore, the table established for light breed horses can be used to assess the foetal age of foetuses carried by recipient mares whether they are WB or STB. Using the same anatomical landmarks but a different ultrasound machine, both ultrasonographers determined foetal age with similar accuracy.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70059"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}