Reproduction in Domestic Animals最新文献

Bull Sperm Morphology Assessment: Agreement Between AI Station and Trumorph. 公牛精子形态评估：AI站与Trumorph的一致。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70251

Francisco Sevilla, Ignacio Araya-Zúñiga, Kenneth Matamoros-Corella, Manuel Barrientos-Morales, Arcesio Salamanca-Carreño, Miguel A Silvestre, Anthony Valverde

{"title":"Bull Sperm Morphology Assessment: Agreement Between AI Station and Trumorph.","authors":"Francisco Sevilla, Ignacio Araya-Zúñiga, Kenneth Matamoros-Corella, Manuel Barrientos-Morales, Arcesio Salamanca-Carreño, Miguel A Silvestre, Anthony Valverde","doi":"10.1111/rda.70251","DOIUrl":"https://doi.org/10.1111/rda.70251","url":null,"abstract":"This study investigated the workflow-level agreement between AI Station and Trumorph systems under practical conditions in bull sperm morphology assessment in 41 ejaculates from 13 Brahman bulls. Agreement was evaluated using mixed-effects models, Bland-Altman plots, and Passing-Bablok regression, applying a ±20% acceptance threshold. Although both systems yielded similar mean values for normal morphology, Bland-Altman analysis demonstrated wide limits of agreement, indicating limited interchangeability at the individual-sample level and substantial disagreement for specific morphoanomalies. AI Station yielded higher estimates of proximal and distal cytoplasmic droplets, whereas Trumorph produced higher estimates of bent-tail and coiled-tail defects. Several traits showed evidence of constant or proportional bias, pointing to systematic differences between the two methods. Overall, these findings indicate that the two systems are not interchangeable under the analytical conditions evaluated here and require platform-specific validation before routine diagnostic or classification use.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70251"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periparturient Changes in Maternal Body Temperature and Colostrum Physicochemical Characteristics in Rasa Aragonesa Ewes. 阿拉贡沙母羊围产期母体体温及初乳理化特性的变化。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70294

L Gil, S Martín, L Horndler, V Luño, N Gonzalez, F Martinez

{"title":"Periparturient Changes in Maternal Body Temperature and Colostrum Physicochemical Characteristics in Rasa Aragonesa Ewes.","authors":"L Gil, S Martín, L Horndler, V Luño, N Gonzalez, F Martinez","doi":"10.1111/rda.70294","DOIUrl":"https://doi.org/10.1111/rda.70294","url":null,"abstract":"Accurate identification of the periparturient period is essential for improving reproductive management in sheep. This study evaluated changes in maternal temperature and colostrum physicochemical characteristics during the transition from late gestation to early lactation. Seventeen pregnant ewes were monitored from 36 h before to 36 h after lambing. Vaginal and rectal temperatures were measured by digital thermometry, udder surface temperature by infrared thermography, and colostrum calcium, Brix value and pH were assessed using rapid field methods. Vaginal and rectal temperatures decreased significantly before lambing (p < 0.001), whereas udder surface temperature remained unchanged. Colostral calcium concentration decreased before parturition and increased postpartum (p < 0.001), while Brix values increased significantly before lambing (p < 0.001). No significant changes were observed in colostral pH. These findings suggest that maternal temperature and colostrum physicochemical characteristics may serve as useful physiological indicators of imminent lambing in ewes.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70294"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Follicle Number and Reproductive Cyclicity on Mesenchymal Stromal Cell Yield Isolated From Equine Ovarian Follicular Aspirates. 卵泡数量和生殖周期对马卵巢抽吸卵泡间充质间质细胞产量的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70306

Carmen C Muñoz-García, María Del Prado Soriano-Campos, Marcos Luis-Calero, Marian Del Olmo-Ortiz, Lauro González-Fernández, Beatriz Macías-García

{"title":"Influence of Follicle Number and Reproductive Cyclicity on Mesenchymal Stromal Cell Yield Isolated From Equine Ovarian Follicular Aspirates.","authors":"Carmen C Muñoz-García, María Del Prado Soriano-Campos, Marcos Luis-Calero, Marian Del Olmo-Ortiz, Lauro González-Fernández, Beatriz Macías-García","doi":"10.1111/rda.70306","DOIUrl":"https://doi.org/10.1111/rda.70306","url":null,"abstract":"Follicular aspirates have emerged as an alternative source of mesenchymal stromal cells (MSC) in mares; however, factors influencing successful MSC isolation remain unclear. This study evaluated whether reproductive cyclicity and follicle count affect MSC recovery from ovum pick-up (OPU)-derived follicular aspirates. Twenty-eight OPU procedures were performed on 13 mares, and cells were cultured until passage 3. Successful MSC cultures were obtained in 15/28 procedures (53.6%), while 13 procedures (46.4%) yielded non-MSC populations. Neither reproductive status (cycling vs. anestrous) nor follicle count influenced MSC culture success. Interestingly, unsuccessful cultures showed significantly higher cell counts at passage 0 compared to successful cultures (p < 0.001). Receiver operating characteristic curve analysis revealed that passage 0 cell count was an excellent predictor of culture failure (AUC = 0.923; p < 0.001), with a threshold of ≥ 700,000 cells. These findings demonstrate that OPU-derived follicular aspirates represent a reliable MSC source and that high initial cell count predicts culture failure, providing a practical early screening tool for MSC production.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70306"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Susceptibility of Donkey and Horse Sperm to Liquid-Storage Temperature. 驴和马精子对液体储存温度的比较敏感性。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70260

Pilar Vallejo-Soto, Antonio González Ariza, Blenda R N Vilela, Carlos Montero-Palma, Rocío Rodríguez González-Miranda, Verónica Pérez-Aguilera, Francisco Crespo-Castejón, Manuel Hidalgo, Jesús Dorado, Isabel Ortiz

{"title":"Comparative Susceptibility of Donkey and Horse Sperm to Liquid-Storage Temperature.","authors":"Pilar Vallejo-Soto, Antonio González Ariza, Blenda R N Vilela, Carlos Montero-Palma, Rocío Rodríguez González-Miranda, Verónica Pérez-Aguilera, Francisco Crespo-Castejón, Manuel Hidalgo, Jesús Dorado, Isabel Ortiz","doi":"10.1111/rda.70260","DOIUrl":"https://doi.org/10.1111/rda.70260","url":null,"abstract":"Preservation protocols for donkey sperm are based on those developed for horses, despite potential interspecific differences in cooling tolerance. This study compared horse and donkey sperm during long-term storage at 5°C and 17°C. Ejaculates from four stallions and four jacks (n = 16) were centrifuged, resuspended in skimmed milk-based extender, and stored at both temperatures. Sperm motility, plasma and acrosome membrane integrity, and morphology were evaluated after 1, 2 and 7 days. Fresh semen parameters showed no interspecific differences (p > 0.05). At 5°C, donkey sperm showed reduced motility and membrane integrity from Day 1, whereas horse sperm parameters remained stable at both temperatures up to Day 2. After 7 days, horse doses stored at 5°C maintained significantly higher quality. In conclusion, donkey sperm was more sensitive to storage at 5°C than horse sperm, highlighting the need for species-specific preservation strategies.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70260"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Gene Expression of Mesenchymal Stromal Cell and Granulosa Cell Markers in Equine Follicular Aspirates. 马毛囊抽吸中间充质间质细胞和颗粒细胞标记物的差异基因表达。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70288

María Del Prado Soriano-Campos, Carmen Cristina Muñoz-García, Marcos Luis-Calero, Marian Del Olmo-Ortiz, Alejandro Gallardo-Soler, Beatriz Macías-García, Lauro González-Fernández

{"title":"Differential Gene Expression of Mesenchymal Stromal Cell and Granulosa Cell Markers in Equine Follicular Aspirates.","authors":"María Del Prado Soriano-Campos, Carmen Cristina Muñoz-García, Marcos Luis-Calero, Marian Del Olmo-Ortiz, Alejandro Gallardo-Soler, Beatriz Macías-García, Lauro González-Fernández","doi":"10.1111/rda.70288","DOIUrl":"https://doi.org/10.1111/rda.70288","url":null,"abstract":"Equine follicular aspirates obtained during ovum pick-up contain a heterogeneous mixture of mesenchymal stromal cells (MSC) and granulosa cells (GC). This study evaluates how selective isolation and expansion of MSC decrease GC populations. Follicular aspirates-derived cells were cultured up to passage four (P4) following a previously validated protocol. While freshly isolated follicular aspirates cells (FAC) displayed heterogeneous morphologies, early passages (P3-P4) showed a uniform, spindle-shaped phenotype compatible with MSC. Gene expression analysis by RT-qPCR revealed a marked upregulation of mesenchymal and stemness-associated genes (FN1, THY1, FGF2 and CD105) in P4-MSC compared with FAC, confirming enrichment of the MSC population. Conversely, GC-specific genes (FSHR, FOXL2 and CYP11A1) were significantly upregulated in FAC. These findings suggest that early passages efficiently promote selective MSC expansion while progressively eliminating GC.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70288"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cinnamaldehyde as a Natural Antimicrobial in Donkey Semen: Efficacy and Sperm Cytotoxicity. 肉桂醛作为驴精液中的天然抗菌剂：功效和精子细胞毒性。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70250

Carlos Montero-Palma, Antonio Romero-Salmoral, Pilar Vallejo-Soto, Blenda Rodrigues Nunes Vilela, Verónica Pérez, Francisco Crespo-Castejón, Isabel Ortiz, Manuel Hidalgo, Jesús Dorado, Inmaculada Luque, Lidia Gómez-Gascón

{"title":"Cinnamaldehyde as a Natural Antimicrobial in Donkey Semen: Efficacy and Sperm Cytotoxicity.","authors":"Carlos Montero-Palma, Antonio Romero-Salmoral, Pilar Vallejo-Soto, Blenda Rodrigues Nunes Vilela, Verónica Pérez, Francisco Crespo-Castejón, Isabel Ortiz, Manuel Hidalgo, Jesús Dorado, Inmaculada Luque, Lidia Gómez-Gascón","doi":"10.1111/rda.70250","DOIUrl":"https://doi.org/10.1111/rda.70250","url":null,"abstract":"Bacterial contamination of donkey semen samples is a major concern due to its deleterious effects on sperm quality; this issue is exacerbated during semen storage. Therefore, evaluating the effect of antimicrobials on sperm is of utmost importance. This study identified microorganisms present in donkey semen and evaluated the antimicrobial activity of cinnamaldehyde, determined by the Minimum Inhibitory Concentration (MIC), and its effects on sperm quality. Fourteen semen samples from seven donkeys were analysed, identifying 31 bacterial isolates representing 21 different species. Cinnamaldehyde showed antimicrobial activity (MIC50 = 0.06 mg/mL; MIC90 = 4 mg/mL). Reactive oxygen species levels in sperm were decreased, suggesting an antioxidant effect. However, high concentrations of cinnamaldehyde reduced sperm motility. In conclusion, cinnamaldehyde is a promising antimicrobial for semen preservation; however, further studies are needed to ensure antimicrobial efficacy and sperm functionality.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70250"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-Weaning Administration of a PGF2α Analogue (Cloprostenol) and Its Influence on Early Reproductive Indicators in Sows Under Commercial Farm Conditions. 断奶前添加PGF2α类似物（氯前列醇）及其对母猪早期繁殖指标的影响

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70290

Sara Crespo, Joaquín Gadea

{"title":"Pre-Weaning Administration of a PGF2α Analogue (Cloprostenol) and Its Influence on Early Reproductive Indicators in Sows Under Commercial Farm Conditions.","authors":"Sara Crespo, Joaquín Gadea","doi":"10.1111/rda.70290","DOIUrl":"https://doi.org/10.1111/rda.70290","url":null,"abstract":"Efficient reproductive management in commercial swine production requires minimising non-productive days and ensuring rapid return to oestrus after weaning. The present study evaluated the effect of pre-weaning administration of cloprostenol, a PGF2α analogue, on early reproductive indicators in sows under commercial farm conditions. A total of 734 clinically healthy multiparous sows (parity 2-9) from a commercial farm were randomly allocated to two groups before weaning. Control sows (n = 367) received no treatment, whereas treated sows (n = 367) received an intramuscular injection of cloprostenol sodium 12 h before piglet removal. Reproductive parameters evaluated included the weaning-to-first-service interval (WFSI), proportion of sows in oestrus within 7 days after weaning, pregnancy rate, early endometritis and abortion rate within the first 42 days of gestation. Sows treated with cloprostenol showed a significantly shorter WFSI compared with control sows (treated: 4.94 ± 0.18 days vs. control: 6.41 ± 0.27 days; p = 0.001) and a higher proportion of oestrus within 7 days after weaning (93.19% vs. 84.2%; p = 0.008). Pregnancy rate and early endometritis incidence were not affected by treatment. However, cloprostenol significantly reduced abortion rate during early gestation (0.55% vs. 3.27%; p = 0.006). Under the conditions of this field study, pre-weaning administration of cloprostenol was associated with a shorter WFSI, a higher proportion of sows showing oestrus within 7 days after weaning, and a lower early abortion rate. These findings suggest that cloprostenol may influence early reproductive indicators in multiparous sows under commercial farm conditions, although further studies including endocrine, ovarian and farrowing outcomes are required.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70290"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Cytokines in Donkey Follicular Fluid and Seminal Plasma: A Pilot Study. 驴卵泡液和精浆中细胞因子的特征：一项初步研究。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70271

Sebastià Company, Jaime Catalán, Lorena Padilla, Carolina Maside, Jordi Miró, Marc Yeste

{"title":"Characterization of Cytokines in Donkey Follicular Fluid and Seminal Plasma: A Pilot Study.","authors":"Sebastià Company, Jaime Catalán, Lorena Padilla, Carolina Maside, Jordi Miró, Marc Yeste","doi":"10.1111/rda.70271","DOIUrl":"https://doi.org/10.1111/rda.70271","url":null,"abstract":"Post-breeding endometrial inflammation is a well-known physiological response in jennies, especially after artificial insemination with frozen-thawed sperm. Bioactive molecules present in reproductive fluids, including cytokines, may contribute to regulating these immune processes. The aim of this study was to characterize the cytokine profile of preovulatory follicular fluid (FF) and seminal plasma (SP) in donkeys and to explore their potential relevance for reproductive physiology. To this end, 10 FF samples were collected from five fertile Catalan jennies, and 10 samples were isolated from 10 different Catalan jackasses' ejaculates. Cytokine concentrations were measured using a Luminex MagPix system with an equine-specific Milliplex MAP panel. Three cytokines were detected in the FF, with IP-10 being the most abundant, followed by IL-10 and eotaxin. The SP, in contrast, showed a broader cytokine profile, with 10 detectable mediators. Fractalkine was the most abundant cytokine in this biofluid, followed by FGF-2 and G-CSF. GRO and IL-1β were present at intermediate concentrations, and IL-8, IL-13 and IL-12 were observed at low concentrations. From the 10 cytokines detected, IP-10 and eotaxin were found at the lowest concentrations. These results provide the first description of cytokines in donkey FF and SP. The presence of chemotactic and immunomodulatory cytokines in these reproductive fluids suggests they may contribute to the regulation of follicular processes and the post-breeding inflammatory response in jennies.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70271"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Genome Editing in Domestic Animals: A New Era for Agriculture and Medicine. 家畜精确基因组编辑：农业和医学的新时代。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70286

Mariana Sponchiado, Paula Mangiavacchi, Bethany K Redel, Kiho Lee

{"title":"Precision Genome Editing in Domestic Animals: A New Era for Agriculture and Medicine.","authors":"Mariana Sponchiado, Paula Mangiavacchi, Bethany K Redel, Kiho Lee","doi":"10.1111/rda.70286","DOIUrl":"https://doi.org/10.1111/rda.70286","url":null,"abstract":"Precision genome editing technologies have transformed the generation and application of genetically engineered livestock, positioning domestic animals as powerful models for agriculture, biomedicine, and basic research. Historically, genetic engineering in livestock was constrained by the absence of embryonic stem cells, low efficiency of homologous recombination, long gestation periods, and extended generation intervals. The advent of programmable nucleases, including zinc finger nucleases, transcription activator-like effector nucleases, and CRISPR-Cas systems, has overcome many of these barriers by enabling efficient targeted genome modification. This review focuses on the application of genome editing technologies in mammalian livestock species, with pigs highlighted as a representative model. We discuss the historical development of livestock genetic engineering and summarise two main routes used to establish genome edited livestock models. Key technical considerations unique to domestic animals are also presented. Genome editing has accelerated the production of livestock models for human disease, xenotransplantation, reproductive biology, and agricultural improvement, including disease resistance, altered growth traits, and enhanced product composition. Emerging epigenome editing strategies further expand the toolkit by allowing locus-specific gene regulation without permanent DNA modification. Despite rapid technological progress, challenges remain, including mosaicism, off-target effects, limited ability to segregate unintended edits, and ethical and regulatory concerns surrounding food and biomedical applications. Continued refinement of genome editing strategies will be essential for maximizing the utility and responsible implementation of genome-edited livestock. Overall, precision genome editing represents a transformative platform with broad implications for sustainable agriculture, translational research, and global food security.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70286"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species. 孵化后胚胎发育的分子调控：对家畜早期妊娠损失的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70225

Alexia Siegmund-Sabater, Nuria Martínez de Los Reyes, Inés Flores-Borobia, Leopoldo González-Brusi, Beatriz Galiano-Cogolludo, Pilar Marigorta, Pablo Bermejo-Álvarez, Priscila Ramos-Ibeas

{"title":"Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.","authors":"Alexia Siegmund-Sabater, Nuria Martínez de Los Reyes, Inés Flores-Borobia, Leopoldo González-Brusi, Beatriz Galiano-Cogolludo, Pilar Marigorta, Pablo Bermejo-Álvarez, Priscila Ramos-Ibeas","doi":"10.1111/rda.70225","DOIUrl":"https://doi.org/10.1111/rda.70225","url":null,"abstract":"Pregnancy loss (PL) remains a major limitation to reproductive efficiency in livestock, with a substantial proportion originating during early embryo development, particularly between blastocyst hatching and implantation. This developmental window encompasses complex processes of lineage differentiation and morphogenesis. Accumulating evidence indicates that intrinsic defects in embryonic development, most notably within the epiblast, are key contributors to PL, especially following in vitro embryo production. Mechanistic investigation of this period has historically been limited by reliance on resource-demanding in vivo approaches. In this review, we summarize current knowledge on the molecular regulation of early embryo development in ungulates and the factors associated with PL during the post-hatching period. We further synthesize recent advances in molecular technologies, extended embryo culture systems, and three-dimensional endometrial models that are transforming the study of post-hatching embryo development in livestock species. Collectively, these advances provide a foundation for refining assisted reproductive technologies and developing predictive strategies to mitigate PL, thereby enhancing reproductive efficiency and sustainability in livestock production systems.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70225"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0