Fertility Outcomes in Lohi Sheep: A Comparative Study of Laparoscopic Versus Trans-Cervical AI With Frozen-Thawed Semen Under Subtropical Conditions.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2025-10-01 DOI:10.1111/rda.70129

Muhammad Tayyab Khan, Ejaz Ahmad, Muhammad Irfan-Ur-Rehman Khan

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引用次数: 0

Abstract

Efficient use of frozen-thawed semen in sheep is limited by low fertility following cervical insemination (CAI), whereas laparoscopic insemination (LAI) offers higher success but requires specialised skills and equipment. The current study aimed to evaluate the fertility outcomes of two artificial insemination techniques, laparoscopic and transcervical, in Lohi sheep using frozen-thawed semen. Lohi ewes (n = 44) were synchronised through progestin-sponges (day 0-day 11), administering PGF2_α (d-cloprostenol 75 μg/mL; i.m @ 75 μg/ewe) on day 09 and eCG (i.m @ 300 IU/ewe) on day 11. Synchronised ewes (n = 44) were divided into two groups for artificial insemination (AI) procedures: cervical (CAI; n = 24; sperm concentration = 200 million/dose) and laparoscopic (LAI; n = 20; sperm concentration = 50 million/dose). All ewes were inseminated at 54 h post-sponge removal. Results of the experiment revealed that LAI demonstrated higher pregnancy rates (65%) than CAI (33.3%) (p = 0.05). However, no significant differences were observed in prolificacy or twinning rates between the two techniques (p = 0.11). In conclusion, LAI as compared to CAI is a superior method of insemination for achieving optimum pregnancy rates in estrous synchronised Lohi ewes.

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Lohi羊的生育结果：亚热带条件下腹腔镜与经宫颈冷冻解冻精液人工受精的比较研究

绵羊冷冻解冻精液的有效利用受到宫颈人工授精（CAI）后低生育率的限制，而腹腔镜人工授精（LAI）成功率更高，但需要专门的技能和设备。目前的研究旨在评估两种人工授精技术（腹腔镜和经宫颈）在Lohi羊中使用冻融精液的生育效果。Lohi母羊（n = 44）通过孕酮海绵同步（第0- 11天），第09天给予PGF2α （d-氯前列醇75 μg/mL； ig @ 75 μg/母羊），第11天给予eCG （ig @ 300 IU/母羊）。将同步母羊（n = 44）分为两组进行人工授精（AI）：宫颈（CAI, n = 24，精子浓度= 2亿/剂）和腹腔镜（LAI, n = 20，精子浓度= 5000万/剂）。所有母羊在海绵去除后54 h进行授精。实验结果显示LAI的妊娠率（65%）高于CAI (33.3%) （p = 0.05）。然而，两种技术在繁殖率和双胞胎率方面没有显著差异（p = 0.11）。综上所述，与CAI相比，LAI是一种更好的授精方法，可以在发情同步的Lohi母羊中获得最佳的受孕率。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.