Reproduction in Domestic Animals最新文献

{"title":"Biopsy of Porcine Blastocysts for Sex Identification and Transcriptome Sequencing Based on Laser-Assisted Microdissection.","authors":"Yan Liu, Ye Li, Wanchen Li, Mengru Ji, Yuting Zhang, Xinghong Ma, Zhonghua Liu, Xiaogang Weng","doi":"10.1111/rda.70087","DOIUrl":"https://doi.org/10.1111/rda.70087","url":null,"abstract":"<p><p>Pre-implantation embryo biopsy is a well-established technique in large livestock like cattle. However, its application in porcine embryos remains relatively limited. In this study, we successfully obtained trophectoderm cells from porcine IVP blastocysts using laser-assisted microdissection. First, we obtained trophectoderm cells of varying cell numbers (Group 1: less than three cells; Group 2: more than five cells) using laser-assisted microdissection and then evaluated the impact of this dissection procedure on embryo morphology. The morphological observation confirmed the complete normal post-biopsy recovery of blastocysts in the two groups. Furthermore, the expression of proteins associated with cell junction and structural integrity remained unaffected. Comparative analysis of blastocyst cell numbers and apoptotic rates among Group 1, Group 2 and the control group further validated the minimalinvasiveness of the laser-assisted microdissection when cutting less than three cells. The quality and utility of the biopsied trophectoderm cells were confirmed through successful sex identification and transcriptome sequencing. In conclusion, this study successfully established a minimal invasive laser-assisted microdissection technique for porcine blastocysts, enabling pre-implantation sex identification and blastocyst quality assessment.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70087"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Prevalence of Equine Coital Exanthema in a Thoroughbred Covering Station in Türkiye (2021-2024).","authors":"Yunus Emre Atay, Gencay Ekinci, Ali Erdem Öztürk, Mustafa Cem Timur, Alper Mete, Köksal Altınbay, Fatih Mehmet Derelli, Yaşar Akar, İhsan Keleş","doi":"10.1111/rda.70086","DOIUrl":"10.1111/rda.70086","url":null,"abstract":"<p><p>Equine Coital Exanthema (ECE) is an endemic herpesvirus disease primarily affecting the external genitalia and impairing mating activities in horses. Its extremely contagious nature, latency and subclinical features can result in outbreaks and significant economic losses. Transmission occurs primarily through mating activities; therefore, robust biosecurity measures are crucial in breeding facilities. This study aims to determine the clinical prevalence of ECE among horses in a covering station in Türkiye from 2021 to 2024. It also aims to assess the efficacy of routine PCR implementation within ECE's control strategies. A cross-sectional study design has been employed. Genital swab samples were collected from clinically suspected horses, which were tested for EHV-3 using real-time PCR. Animal records, clinical examination data and PCR test results were obtained from horses at the covering station between 2021 and 2024. During the 4 years (2021-2024), 9231 mating activities were carried out, and a total of 228 clinically suspected horses were tested for EHV-3 using real-time PCR. Among these 228 horses, 6 horses (2.6%) were confirmed positive for EHV-3. The primary weakness of this study is the failure to detect subclinical cases with PCR. The absence of follow-up PCR testing in two clinically infected horses represents a limitation of this study. The molecular diagnosis of ECE was reported for the first time in Türkiye. Clinical ECE cases infrequently transpired over the four-year period at the covering station. No outbreak transpired during this interval. PCR testing plays a crucial role in disease control when implemented with suitable management methods. Additional global epidemiological investigations on ECE are required.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 6","pages":"e70086"},"PeriodicalIF":1.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine Congenital Defects Recorded in a National Survey of Dairy and Beef Herds Over Ten Years (2014-2023).","authors":"Katie Quigley, John F Mee","doi":"10.1111/rda.70067","DOIUrl":"https://doi.org/10.1111/rda.70067","url":null,"abstract":"<p><p>This study describes a novel model of farm-based congenital defect recording, with use of accessory data from a national breeding organisation. The study ran from 2014 to 2023 inclusive resulting in 522 reports of bovine congenital defects with additional data available for a subset (369/522). The most commonly reported defect was intestinal atresia (24.3%), followed by multiple defects (19.9%), and tail abnormalities (13.4%). This is the first national survey of bovine congenital defects in Ireland. It shows that while farmer-reported cases generally lack a veterinary clinical or necropsy examination, farmers are in the unique position of being able to report phenotypic abnormalities first-hand, and provide an unbiased perspective of the true typology and epidemiology of congenital defects on farm.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70067"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Signatures of Vulval Skin Surface: A Potential Non-Invasive Diagnostic Technology to Monitor the Calving Process in Water Buffalo (Bubalus bubalis).","authors":"Allu Teja, Jeyakumar Sakthivel, Kancharana Ananda Rao, Narayanan Krishnaswamy, Gowtham Varma Chintalapati, Vedamurthy Gowdar Veerappa, Arumugam Kumaresan, Kerekoppa P Ramesha, Muniandy Sivaram, Mukund A Kataktalware, Dayal N Das, Ravi Kumar Mula, Maharajan Lavanya","doi":"10.1111/rda.70065","DOIUrl":"https://doi.org/10.1111/rda.70065","url":null,"abstract":"<p><p>For the first time, the possibility of using infrared thermography to capture the temperature of the vulval skin surface around the calving process in order to forecast when the buffaloes' calving process will begin has been investigated. FLUKE infrared thermal camera was used to record the vulval skin surface temperature (VSST) and ocular temperature. Twenty-eight multiparous pregnant buffaloes had their thermograms taken at 6-h intervals between the 96th hour before the anticipated calving and the 24th hour after the calving. The eye and vulval skin surface showed a ΔT of 0.56°C and 0.45°C, respectively, indicating a considerable drop in temperature from 48 h before to calving. On the other hand, starting 6 h before calving, the vulval skin temperature showed an upward trend. There was minimal effect of circadian influence on the vulval skin surface temperature, as evidenced by the identical and significant declining pattern of the residual temperature of both the eye and VSST from 48 to 0 h of calving. Nonetheless, the reduction is ascribed to progesterone, which lowers vulval skin vascular perfusion and cutaneous circulation. Our study's findings suggested that, using related thermal signatures, vulval skin surface temperature variation in relation to calving as observed by digital infrared thermal imaging could be explored as a potential tool for predicting the onset of impending calving in buffaloes within -48 h.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70065"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance and Control of Colostrum Intake in the Neonate Pig, a Review.","authors":"P Langendijk","doi":"10.1111/rda.70077","DOIUrl":"10.1111/rda.70077","url":null,"abstract":"<p><p>This review discusses the biological significance of colostrum intake for neonatal development in pigs, and discusses the variation in colostrum intake in relation to piglet and sow factors. Lack of or insufficient colostrum intake reduces the odds of survival and compromises immunity, development of the gastrointestinal tract and other organs in neonate piglets. Colostrum intake is linearly related to pre-weaning gain. It is estimated that 10%-30% of piglets consume insufficient colostrum (< 250 g). Colostrum intake is less in piglets from larger litters, in piglets born later in the birth order, in piglets with low birth weight, and in piglets mildly or severely affected by asphyxia. Colostrum yield by sows peaks between parity two and five and then declines again, and is probably related to mammary gland tissue mass and endocrine factors involved in colostrogenesis. Before farrowing, the decline in progesterone facilitates and the increase in prolactin initiates secretion of colostrum, and the changes in both these hormones are related to colostrum yield. Feeding level and fibre may accelerate the decline in progesterone and in that way increase colostrum yield. Earlier during the third trimester of pregnancy, proper management of energy intake and sow body condition is important to optimise mammary gland development. Specific amino acids may be involved in mammary gland development, potentially through IGF-1.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70077"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Short-Term Timed Artificial Insemination Protocols With Estradiol Esters and PGF2α in Girolando Cows.","authors":"Mariana Dos Santos Dutra Okada, Lara Nogueira Silenciato, Samuel Rodrigues Bonamichi do Couto, Joaquim Esquerdo Ferreira, Marco Roberto Bourg de Mello","doi":"10.1111/rda.70076","DOIUrl":"10.1111/rda.70076","url":null,"abstract":"<p><p>Using a synchronisation protocol based on estradiol esters and prostaglandin, established in cattle since the 1970s, we implemented 24 or 48-h intervals between hormone administration and timed artificial insemination (TAI). This study compared two short-duration TAI protocols using estradiol benzoate (EB) and cypionate (EC) as ovulation inducers. A total of 172 Girolando female cows were selected after ovarian ultrasonographic evaluation and received 500 μg of prostaglandin F2α (PGF) at the start of the treatment (D0). The inclusion criterion for females in the study was the presence of a corpus luteum measuring ≥ 14 mm and at least one follicle with a diameter between 8 and 20 mm. The cows were randomly sorted into two treatment groups. The EB group received 2 mg of EB 24 h after PGF (D1), and TAI was performed 24 h later (D2). The EC group received 2 mg estradiol cypionate simultaneously with PGF, and TAI was performed 48 h later. Pregnancy was diagnosed 30 days after AI using transrectal ultrasonography. Experiment I evaluated follicular dynamics in 18 females (EB: n = 9; EC: n = 9) by analysing the follicular growth rate, pre-ovulatory follicle (POF) diameter, ovulation timing and ovulation rate. No statistically significant differences were observed (p > 0.05), with a 77.8% ovulation rate in both treatments. Experiment II compared the conception rates in 172 females (EB: n = 85; EC: n = 87). The overall conception rate was 30.2%, with no significant difference between the treatments (EB: 27.1%; EC: 33.3%). However, cows with a body condition score < 3.0 tended to have higher conception rates with EC than with EB (39.1% vs. 18.2%; p = 0.06). Cows with POF ≥ 10 mm at D0 had significantly higher conception rates with EC than with EB (48.3% vs. 29.0%; p = 0.03). Both protocols were equally effective; however, EC was more advantageous for cows with a POF ≥ 10 mm at treatment initiation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70076"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lectin-Histochemical Pattern on the Cystic and Neoplastic Ovaries of Bitches.","authors":"Juliano P Terra, Thiago B Almeida, Lucas P O Rezende, Claudio G Barbeito, Francisco Acuña, Guilherme R Blume, Rômulo S A Eloi, Letícia B Oliveira, André L R M Santos, Fabiano J F Sant'Ana","doi":"10.1111/rda.70075","DOIUrl":"10.1111/rda.70075","url":null,"abstract":"<p><p>Studies have demonstrated that many reproductive disorders can compromise the binding pattern of glycosidic residues, mainly in cows and sows. In the current study, the binding pattern of lectins was characterised and compared in normal and pathological ovaries of bitches. Fourteen biotinylated lectins (BSA-1B, Con-A, DBA, LCA, PHA-E, PHA-L, PNA, RCA-1, SBA, SJA, STA, WGA, s-WGA, UEA-1) were used. In ovaries without lesions, the predominant staining for RCA-1 was observed in the zona pellucida (ZP) of initial follicles, in the oocytes of primordial and primary follicles, and in the artery wall. For WGA, the reaction predominated in the mesothelium, in the ZP of secondary follicles, in the corpus luteum (CL) and in the oocyte of secondary follicles. Reactivity for STA was more pronounced in the mesothelium and ZP of developing follicles. The mesothelial cells and the CL were mainly marked for LCA. PHA-L was stained in the mesothelium, CL, and connective stroma, while the lectin PHA-E showed reactivity especially in the stroma, mesothelium, and artery wall. In the cystic ovaries, changes in the reaction of some lectins were noted in various structures adjacent to the cysts. RCA-1 staining was reduced in the ZP and oocyte of primordial, primary, and secondary follicles, while there was an increase of staining in endothelial cells. With WGA lectin, there was a reduction in CL and an increase in endothelial cells. Reactivity to STA was reduced in the ZP of primary and secondary follicles and increased in endothelial cells. LCA was also reduced in the CL and ZP of primary and secondary follicles. For the PHA-L lectin, the staining was reduced in the mesothelial cells, CL, and ZP of the primary follicles, while for PHA-E the reduction occurred in the stroma, oocyte of the primary follicles, and in the GC of the secondary follicles. In all neoplasms analysed (dysgerminoma, granulosa cell tumour, adenocarcinoma), there was also a reduction in reactivity to various lectins, both in the neoplastic cells compared to the cells of origin and in the adjacent non-neoplastic ovarian tissue. The data from the current study suggest that the changes in ovarian glycosidic residues observed in cysts and neoplasms may contribute to infertility in bitches.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70075"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycerol Supplementation During Oestrus Synchronisation With Fluorogestone Acetate Affects Follicular Proliferation, Maturation and Apoptosis of Granulosa Cells in Pelibuey Ewes.","authors":"P Escorcia Ortiz, C A Price, A Guzmán Sánchez, H M Guerrero-Netro","doi":"10.1111/rda.70080","DOIUrl":"10.1111/rda.70080","url":null,"abstract":"<p><p>This study investigated the impact of glycerol supplementation on the expression of markers associated with follicular proliferation, maturation and apoptosis in Pelibuey ewes synchronised with long (11 days) and short (5 days) protocols using intravaginal fluorogestone acetate (FGA). Forty-five adult Pelibuey ewes were divided into five groups: control, short protocol with or without glycerol and long protocol with or without glycerol. Follicular dynamics were monitored by ultrasonography, and granulosa cells were collected 15 h after the onset of oestrus to analyse gene expression using RT-qPCR of genes associated with proliferation, maturation, and apoptosis. Results indicated that glycerol supplementation or the duration of the synchronisation protocol influenced the expression of genes related to apoptosis, proliferation and maturation. Specifically, glycerol-treated groups exhibited decreased BCL2 expression, and the ratio BCL2/BAX was lower in all treatment groups compared to the control, particularly in the short protocol plus glycerol. We conclude that the use of glycerol as an ultrashort supplementation during different synchronisation protocols leads to a delay in maturation and induces apoptotic-related pathways in granulosa cells to granulosa cell likely reducing the oocyte viability. Finally, glycerol treatment does not increase the abundance of PCNA associated with granulosa cell proliferation. However, it is observed that short-term treatments promote proliferation compared to the control group.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70080"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Ram Semen Quality After Supplementation With Gelatin, Agar or Alginate Prior to Cooling Storage\".","authors":"","doi":"10.1111/rda.70063","DOIUrl":"https://doi.org/10.1111/rda.70063","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70063"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-Related Changes in Accessory Sex Organs of Egyptian Jacksassess (Equus asinus): A Morphometric and Histological Study.","authors":"Ahmed Sabry S Abdoon, Afaf A Kishta, Nazema S Abdel-Megeid, Shenming Zeng, Said I Fathalla, Sherif M Shawky, Igor F Canisso, Shimaa R Masoud","doi":"10.1111/rda.70082","DOIUrl":"https://doi.org/10.1111/rda.70082","url":null,"abstract":"<p><p>The accessory sex organs play a crucial role in reproductive function, yet limited research exists on age-related changes in these structures in jacks. In the present work, the accessory sex organs of 60 Egyptian jacks were classified according to their age into young (n = 15), adult (n = 25) and senile (n = 20). Jacks were euthanized and the accessory sex organs were immediately separated and collected. Morphometric measures of the penis, ampulla, seminal vesicles, prostate and bulbourethral gland, including length and weight, were determined. Tissue samples from the ampulla, seminal vesicles, prostate and bulbourethral glands were collected, processed and stained with H&E for histomorphometric examination. Our findings reveal significant age-related alterations in the penial weight and length, the structure and secretory activity of the accessory sex glands, shedding light on the physiological changes that occur with advancing age in this species. Insights gained from this analysis can improve reproductive management practices and contribute to the conservation of donkey populations.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70082"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}