Reproduction in Domestic Animals最新文献_第2页

Effect of Different Equilibration Times in the Freezing Process on Post-Thaw Motility Parameters in Equine Spermatozoa. 冷冻过程中不同平衡时间对马精子解冻后运动参数的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70327

Hamed Farnia, Paola Sautejeau-Rizzoni, Mouna Chekchouk, Rédha Belala, Nora Mimoune, Lucas Teixeira Fangueiro, Chantal Thorin, Ingrid Leborgne, Morgane Le-Guiriec, Djemil Bencharif

{"title":"Effect of Different Equilibration Times in the Freezing Process on Post-Thaw Motility Parameters in Equine Spermatozoa.","authors":"Hamed Farnia, Paola Sautejeau-Rizzoni, Mouna Chekchouk, Rédha Belala, Nora Mimoune, Lucas Teixeira Fangueiro, Chantal Thorin, Ingrid Leborgne, Morgane Le-Guiriec, Djemil Bencharif","doi":"10.1111/rda.70327","DOIUrl":"10.1111/rda.70327","url":null,"abstract":"Cryopreservation of stallion semen remains suboptimal, with approximately one-third of stallions producing semen that is poorly tolerant to freezing. As optimization of pre-freezing procedures may improve post-thaw sperm quality, this study evaluated the effect of different equilibration times before freezing on the post-thaw quality of stallion semen. For this purpose, 21 ejaculates were initially collected from 5 stallions and 15 were included in the final analysis after predefined exclusions. These ejaculates were separated from the seminal plasma via centrifugation in INRA 96 medium. After removal of the supernatant, each ejaculate was divided into three aliquots and equilibrated at 4°C for 2, 4, or 6 h before freezing. The pellets were mixed with INRA FREEZE solution at a concentration of 100 million spermatozoa per millilitre. Subsequently, the semen was frozen in 0.5 mL straws after being kept at the designated temperature at +4°C for either 2, 4 or 6 h post-cooling. Post-thaw sperm total motility, progressive motility and kinematic parameters were assessed by computer-assisted sperm analysis (CASA). Total motility increased with equilibration time, from 27% ± 7% after 2 h to 34% ± 10% after 4 h and 40% ± 7% after 6 h (p < 0.05). Progressive motility was significantly lower after 2 h (15% ± 6%) than after 4 h (20% ± 7%) and 6 h (22% ± 7%) of equilibration (p < 0.05), whereas no significant difference was observed between 4 and 6 h. In contrast, equilibration time did not significantly affect sperm kinematic parameters, including VAP, VSL, VCL, ALH and LIN. These findings indicate that extending the equilibration period to 6 h at 4°C before freezing improves post-thaw total and progressive sperm motility in stallions without altering the movement characteristics of surviving spermatozoa.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 10","pages":"e70327"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Multiparametric Flow Cytometry Approach to Study Metabolism in Cumulus Cells. 一种新的多参数流式细胞术研究积云细胞的代谢。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70252

Marc Torres-Garrido, Marc Llavanera, Marc Yeste, Yentel Mateo-Otero

引用次数: 0

Estrous Sheep Serum-Derived Extracellular Vesicles Deliver Proteins That Modulate Sperm Motility in Sheep. 发情绵羊血清来源的细胞外囊泡传递调节绵羊精子运动的蛋白质。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70293

Clara Vidal-Garcia, Marta Neila-Montero, Eva Díaz-Hernández, Paula Bóveda, Carmen María Picazo-Córdoba, Vidal Montoro, Olga García-Álvarez, José Julián Garde, Ana Josefa Soler, María Iniesta-Cuerda

{"title":"Estrous Sheep Serum-Derived Extracellular Vesicles Deliver Proteins That Modulate Sperm Motility in Sheep.","authors":"Clara Vidal-Garcia, Marta Neila-Montero, Eva Díaz-Hernández, Paula Bóveda, Carmen María Picazo-Córdoba, Vidal Montoro, Olga García-Álvarez, José Julián Garde, Ana Josefa Soler, María Iniesta-Cuerda","doi":"10.1111/rda.70293","DOIUrl":"https://doi.org/10.1111/rda.70293","url":null,"abstract":"Sperm capacitation requires signals from the female reproductive environment, although the molecular mechanisms governing this process remain poorly understood. Estrous sheep serum (ESS) is widely used in in vitro fertilization of small ruminants, contributing to mimic the female reproductive fluids. Among ESS components, extracellular vesicles (ESS-EVs) may mediate the transfer of molecular signals to spermatozoa and support the modifications observed when sperm are incubated with ESS. To explore this possibility, ESS-EVs were isolated and validated according to MISEV2023 guidelines, and their proteome was characterized and compared with the protein signature of sperm incubated with ESS. In addition, sperm were co-incubated with ESS-EVs, and sperm motility and mitochondrial activity were assessed. Notably, 27.1% of proteins previously reported to be upregulated in capacitated ram sperm were identified in ESS-EVs, representing a significant enrichment of capacitation-related factors. Functionally, ESS-EVs modulated sperm kinematics, producing a motility profile comparable to that observed under capacitation conditions, while mitochondrial activity remained unchanged. Together, these findings suggest that ESS-EVs may act as molecular conveyors delivering proteins that reshape sperm motility.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70293"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial. 社论。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70320

Emilio A Martinez

引用次数: 0

Clinical, Biochemical and Pathological Findings in Symptomatic and Asymptomatic Bitches With Intrauterine Content. 有症状和无症状母狗宫内内容物的临床、生化和病理表现。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70245

María Victoria Falceto, Ana María Garrido, Alba Garrigós, María Borobia, Bernardino Moreno, María Teresa Tejedor, Cristina Albisu, Laura Garza-Moreno, Julia Cantin, Olga Mitjana

引用次数: 0

Effects of Zinc Oxide Nanoparticles on Post-Thaw Functional, Biochemical and Ultrastructural Characteristics of Cryopreserved Beetal Buck (Capra hircus) Spermatozoa. 氧化锌纳米颗粒对山羊冷冻精子解冻后功能、生化和超微结构的影响

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70326

Soihem Diana Rongmei, Mohua Das Gupta, Keshav Yogi, W Lomen Singh, Kanchan Joshi, Sudip Sinha, Arijit Shome, Maradona Nath, Mitali Dutta Choudhury, Arpana Das, Shantanu Tamuly

{"title":"Effects of Zinc Oxide Nanoparticles on Post-Thaw Functional, Biochemical and Ultrastructural Characteristics of Cryopreserved Beetal Buck (Capra hircus) Spermatozoa.","authors":"Soihem Diana Rongmei, Mohua Das Gupta, Keshav Yogi, W Lomen Singh, Kanchan Joshi, Sudip Sinha, Arijit Shome, Maradona Nath, Mitali Dutta Choudhury, Arpana Das, Shantanu Tamuly","doi":"10.1111/rda.70326","DOIUrl":"https://doi.org/10.1111/rda.70326","url":null,"abstract":"Cryopreservation of buck semen is associated with excessive reactive oxygen species generation, oxidative stress and cellular damage, leading to reduced post-thaw sperm quality. This study evaluated the effects of zinc oxide nanoparticles (ZnO-NPs) added to a Tris-egg yolk-citric acid extender on the post-thaw functional, biochemical and ultrastructural characteristics of Beetal buck spermatozoa. Ten independent pooled ejaculates were equally divided among four treatment groups containing 0 (control), 0.05, 0.10 and 0.20 mg/mL ZnO-NPs before cryopreservation. Post-thaw sperm quality was evaluated using conventional and fluorescence-based assays, including motility, plasma membrane and acrosomal integrity, viability, DNA integrity, mitochondrial membrane potential, superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration. Transmission electron microscopy (TEM) was used to assess sperm ultrastructure in the control and 0.10 mg/mL ZnO-NP groups. ZnO-NP supplementation significantly improved post-thaw sperm motility, plasma membrane functionality, viability, acrosomal integrity, SOD activity, DNA integrity and mitochondrial membrane potential, while reducing MDA concentration compared with the control (p < 0.01). Sperm abnormalities were not significantly affected by ZnO-NP supplementation. Among the tested concentrations, 0.10 mg/mL ZnO-NPs showed the most favourable numerical profile across the evaluated parameters. TEM further indicated better preservation of the plasma membrane, acrosome, mitochondrial sheath and axonemal organization in the 0.10 mg/mL group than in the control. Overall, ZnO-NP supplementation was associated with improved post-thaw functional and ultrastructural sperm quality and a more favourable oxidative status in cryopreserved Beetal buck semen.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 10","pages":"e70326"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Low Concentration of a Sustainably Obtained Blueberry Extract Improves the Post-Thawing Motility of Cryopreserved Bull Spermatozoa. 低浓度可持续获得的蓝莓提取物改善冷冻保存公牛精子的解冻后活力。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70238

Gabriela García-Blanco, Cristina Fra-Hernández, Joana F do-Vale-Rabaça, Laura Pariente-Martín, Marta Veza-Cuenca, Estela Fernández-Alegre, Beatriz Martín-Fernández, J Néstor Caamaño, J Ramiro González-Montaña, Marta Lores, Felipe Martínez-Pastor

{"title":"A Low Concentration of a Sustainably Obtained Blueberry Extract Improves the Post-Thawing Motility of Cryopreserved Bull Spermatozoa.","authors":"Gabriela García-Blanco, Cristina Fra-Hernández, Joana F do-Vale-Rabaça, Laura Pariente-Martín, Marta Veza-Cuenca, Estela Fernández-Alegre, Beatriz Martín-Fernández, J Néstor Caamaño, J Ramiro González-Montaña, Marta Lores, Felipe Martínez-Pastor","doi":"10.1111/rda.70238","DOIUrl":"https://doi.org/10.1111/rda.70238","url":null,"abstract":"Natural extracts could improve sperm storage and artificial insemination (AI). This study, for the first time, evaluates the suitability of a blueberry extract (Vaccinium corymbosum), obtained from pomace using a sustainable methodology, as a supplement for bull semen extenders. Cryopreserved semen doses from eight bulls were combined in 9 pools (3 bulls/pool), supplemented with 0%, 1%, 5%, or 10% extract and incubated up to 5 h at 38°C. Motility was assessed hourly using OpenCASA, and the effects of treatment and time were evaluated using linear mixed-effects models. Motility was significantly better preserved with 1% extract (total and progressive motility, linear velocity and linearities, with lower BCF and fractal dimension, related to hyperactivation). The effect of 5% was overall positive but lower than 1%, whereas 10% showed a mostly negative effect. This natural extract could safely supplement bull semen extenders by at least 5% and even help improve sperm motility. Therefore, its use as a supplement in bull semen extenders offers an opportunity to enhance assisted reproductive techniques in cattle using a sustainable approach.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70238"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Administration of Moringa oleifera or Its Nanoemulsion Ameliorates Testicular Hemodynamics, Testosterone Concentration, Semen Quality, and Total Antioxidant Capacity in Heat-Stressed Goat Bucks. 口服辣木或其纳米乳改善热应激雄山羊睾丸血流动力学、睾酮浓度、精液质量和总抗氧化能力。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70322

Aya M Fadl, Eman Fayez, Fady Sayed Youssef, Mayada Essam, Mahmoud Saber Abdulkader, Ola Adel

{"title":"Oral Administration of Moringa oleifera or Its Nanoemulsion Ameliorates Testicular Hemodynamics, Testosterone Concentration, Semen Quality, and Total Antioxidant Capacity in Heat-Stressed Goat Bucks.","authors":"Aya M Fadl, Eman Fayez, Fady Sayed Youssef, Mayada Essam, Mahmoud Saber Abdulkader, Ola Adel","doi":"10.1111/rda.70322","DOIUrl":"https://doi.org/10.1111/rda.70322","url":null,"abstract":"This study aimed to investigate the novel application of unformulated Moringa oleifera (MOR) and its nanoemulsion (MO-NE) in mitigating the adverse impacts of thermal stress on testicular hemodynamics, the antioxidant profile, and semen characteristics in bucks. Fifteen sexually mature bucks were randomly assigned to three equal groups and treated orally once daily for 8 weeks: (1) the control group (n = 5; CON) received a basal diet only; (2) the MOR group (n = 5; MOR) received the basal diet supplemented with 40 mg/kg BW of unformulated MOR orally; and (3) the moringa nanoemulsion group (n = 5; MO-NE) received the basal diet supplemented with 4 mg/kg BW of MO-NE orally. Testicular ultrasound, semen, and blood parameters were evaluated weekly across 8 weeks (1-8 week) during summer heat-stress (HS) trial (temperature-humidity index (THI) = 76.70-79.54), following a two-week (week-1 and week-2) pre-heat stress winter baseline in February. In the MO-NE group, testicular Doppler indices (resistive index, pulsatility index, and systolic/diastolic ratio) significantly decreased (p < 0.05) from weeks 2-8, concurrent with increased (p < 0.05) total antioxidant capacity, catalase enzyme, superoxide dismutase, and glutathione peroxidase. Furthermore, MO-NE achieved the highest (p < 0.05) testosterone and nitric oxide levels (weeks 2-8), improving sperm motility, sperm viability, acrosome integrity, and normal morphology (weeks 2-8), alongside sperm cell concentration at weeks 5 and 8. In conclusion, MO-NE intervention serves as a viable strategy to mitigate HS in bucks, successfully restoring core reproductive functions, including testicular perfusion and sperm quality parameters.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 10","pages":"e70322"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does the Use of a Sanitary Sheath During Artificial Insemination Improve Pregnancy Outcomes in Cattle? A Systematic Review With Meta-Analysis. 人工授精时使用卫生套能提高牛的妊娠结局吗？荟萃分析的系统评价。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70325

César Augusto Pinzón-Osorio, Alondra González-Patiño, Daniela Cortes-Beltran

{"title":"Does the Use of a Sanitary Sheath During Artificial Insemination Improve Pregnancy Outcomes in Cattle? A Systematic Review With Meta-Analysis.","authors":"César Augusto Pinzón-Osorio, Alondra González-Patiño, Daniela Cortes-Beltran","doi":"10.1111/rda.70325","DOIUrl":"10.1111/rda.70325","url":null,"abstract":"Sanitary sheaths (SS), used to reduce reproductive-tract contamination during bovine artificial insemination (AI), have shown inconsistent associations with pregnancy per AI (P/AI). This systematic review with meta-analysis evaluated whether SS use improves P/AI compared with AI without SS. The primary analysis comprised six independent cohorts contributing seven P/AI strata and 11,367 inseminations. Risk ratios (RRs) were pooled using random-effects models with restricted maximum likelihood estimation and Hartung-Knapp adjustment. The use of SS did not significantly improve P/AI (RR = 1.07; 95% CI: 0.94-1.21; p = 0.245), although the point estimate corresponded to approximately 3.5 additional pregnancies per 100 inseminations. Heterogeneity was moderate (I2 = 43.7%), and the 95% prediction interval crossed the null (RR 0.83-1.36), indicating that the effect may vary across production settings. Sensitivity analyses did not provide robust evidence of benefit: point estimates ranged from RR 1.07 to 1.10, statistical significance depended on dataset composition, and all prediction intervals crossed the null. Exploratory subgroup analyses showed no evidence that the effect differed by AI method, production system, or protective-sheath type. Overall, current evidence does not demonstrate that SS use improves bovine P/AI and does not support its routine use as an effective fertility-enhancing intervention; any potential benefit, if present, may be restricted to sanitary and management conditions that remain insufficiently characterised. Future adequately powered randomised trials are needed to determine whether reducing contamination through SS use causally improves fertility.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 10","pages":"e70325"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13664662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentially expressed miRNA Cargo From Boar Seminal Extracellular Vesicles Is Linked With Motility Quality in AI Doses. 猪精细胞外囊泡差异表达的miRNA载货量与AI剂量下运动质量相关

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70221

Adrián Martín-San Juan, Claudia Cerrato Martín-Hinojal, Helena Nieto-Cristóbal, Maria José Martinez-Alborcia, Eduardo de Mercado, Manuel Álvarez-Rodríguez

引用次数: 0