Reproduction in Domestic Animals最新文献

{"title":"Correction to \"Ultrasonic Evaluation of the Corpus Luteum in Alpacas With Embryo Removal on Day 9 Post-Mating\".","authors":"","doi":"10.1111/rda.70056","DOIUrl":"10.1111/rda.70056","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70056"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wine Grape Pomace as a Dietary Supplement to Improve Semen Quality in Boars.","authors":"Armando Quintero-Moreno, Cristina Martínez-López, Chiara Luongo, María Luz García, María José Argente, Gema Romero, Cristina Pérez-Patiño, José Ramón Díaz, Francisco A García-Vázquez, Pedro José Llamas-López","doi":"10.1111/rda.70049","DOIUrl":"10.1111/rda.70049","url":null,"abstract":"<p><p>Boar spermatozoa are highly susceptible to oxidative damage due to their high content of unsaturated fatty acids, which are prone to disruption by reactive oxygen species (ROS). Excessive ROS can induce lipid peroxidation, DNA fragmentation and impaired enzyme activity, ultimately reducing sperm quality and reproductive performance. Wine grape pomace (WGP), a by-product of the winemaking process, is rich in polyphenols, including flavonoids (anthocyanins and quercetin), stilbenes (resveratrol) and tannins, which possess strong antioxidant properties. This study aimed to evaluate the effects of dietary supplementation with 4% WGP on boar ejaculate output and sperm quality during storage. Twenty boars were divided into two groups: a control group fed a standard diet and a WGP group supplemented with 4% WGP for 4 months. Semen samples were collected and analysed for ejaculate number of sperm per ml, total antioxidant capacity (in seminal plasma) and quality parameters (motility, kinematic parameters, mitochondrial activity, acrosome integrity, viability) after 1, 3 and 5 days of refrigerated storage. Results showed that WGP supplementation increased the number of sperm per ml compared to the control group (p < 0.05), resulting in approximately two additional seminal doses per ejaculate, without negatively affecting other seminal parameters (p > 0.05) and refrigeration storage (p > 0.05). This improvement in sperm concentration could enhance the profitability of swine semen production by increasing the number of doses produced per boar annually. Given the low cost of WGP (10.03 €/boar/year), this strategy could offer a cost-effective approach to improving reproductive performance in boars. These findings support further research into optimising WGP inclusion levels and exploring its broader impacts on boar fertility and reproductive efficiency.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70049"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic Acid Increases Follicle Survival, Stromal Cell Density, Levels of Thiol and Glutathione Peroxidase Activity in Cultured Bovine Ovarian Tissues.","authors":"Pedro A A Barroso, Venância A N Azevedo, Danisvânia R Nascimento, Ernando I T de Assis, Miguel F De Lima Neto, Alesandro S Ferreira, Solano D Martins, Anderson W B Silva, Valdevane R Araújo, José R V Silva","doi":"10.1111/rda.70053","DOIUrl":"10.1111/rda.70053","url":null,"abstract":"<p><p>This study aimed to investigate the radical scavenging activity and the effects of chlorogenic acid on follicular survival and development, stromal cell density and collagen fibres distribution in extracellular matrix, as well as on mRNA expression of nuclear factor erythroid 2-related factor 2 (NRF2), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and periredoxin 6 (PRDX6), thiol levels, and activity of SOD, CAT, GPX enzymes in cultured bovine ovarian tissues. The free radical scavenging potential of chlorogenic acid was assessed through DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. The ovarian fragments were cultured in α-MEM⁺ alone or supplemented with 25, 50, 100, and 200 μmol/L chlorogenic acid for 6 days at 38.5°C and 5% CO₂. Follicular growth and survival, stromal cell density, and collagen fibres were analysed by classical histology. Expression of mRNA for NRF2, SOD, CAT, GPX, and PRDX6 was evaluated by real-time PCR. Thiol levels and activity of SOD, CAT, GPX were investigated by Bradford method. The results showed that the presence of 100 μmol/L chlorogenic acid in culture medium promotes an increase in morphologically normal follicles and stromal cell density when compared to tissues cultured in control medium. Chlorogenic acid also increased thiol levels and GPX activity, but did not influence the distribution of collagen fibres or the expression of the analysed mRNAs. In conclusion,100 μmol/L chlorogenic acid increases the percentage of morphologically normal follicles, stromal cell density, thiol levels, and GPX activity in bovine ovarian tissues cultured in vitro.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70053"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Curcumin Oil Nano-Emulsion on Buffalo Sperm Cryopreservation: Insights on Sperm Quality, Kinematic Parameters, Sperm Ultrastructure, Oxidative Stress, Apoptosis, Microbiota, and Molecular Docking Analysis and Fertilising Capacity.","authors":"Wael A Khalil, Mahmoud A E Hassan, Mohamed M Hegazy, Khaled Fathy, Mostafa A El-Harairy, Aya A Ismail, Mohammed A Alfattah, Ramya Ahmad Sindi, Sameh A Abdelnour","doi":"10.1111/rda.70062","DOIUrl":"https://doi.org/10.1111/rda.70062","url":null,"abstract":"<p><p>This study investigated the potential of incorporating curcumin oil nano-emulsion (CONE) into a Tris-based freezing extender to relieve cryodamage effects and enhance sperm cryo-resistance in buffalo bulls. Pooled semen from five fertile buffalo bulls (n = 40 ejaculates) was divided and supplemented with 0.5% CONE (CONE0.5), 1% CONE (CONE1), or without CONE (CONE0, control), then extended and packaged. Post-thaw assessments included sperm kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, and conception rates. The CONE0.5 group exhibited significantly higher sperm viability, progressive motility, and membrane integrity, as well as significantly lower nitric oxide and MDA levels, compared to the CONE0 and CONE1 groups (p < 0.05), indicating reduced oxidative stress. Compared to the CONE0 group, the CONE0.5 group exhibited a significantly higher percentage of viable and early apoptotic sperm, and a significantly lower percentage of late apoptotic sperm (p < 0.05). Both CONE-treated groups demonstrated significantly lower post-thaw total bacterial, spore-forming bacterial, and coliform bacterial counts (p < 0.05). Conception rates were higher in the CONE0.5 group (80.0%) compared to the CONE0 group (68.0%) (p > 0.05). Molecular docking analysis revealed strong binding affinities between curcumin and caspase-3 (-7.87 kcal/mol), HSP70 (-8.52 kcal/mol), and PRDX-1 (-8.90 kcal/mol), suggesting potential mechanisms of action. In summary, the inclusion of 0.5% CONE as a cryoprotective additive in buffalo semen freezing media demonstrates a potential for improving post-thaw semen quality parameters, suggesting a possible enhancement of reproductive efficiency.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70062"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HSP90 Is Required for Meiotic Resumption and Spindle Formation in Porcine Oocytes.","authors":"Jae-Min Sim, Hyeon-Ji Song, Song-Hee Lee, Xiao-Han Li, Cheng-Lin Zhan, Qin-Yue Lu, Ji-Dam Kim, Gyu-Hyun Lee, Xiang-Shun Cui","doi":"10.1111/rda.70060","DOIUrl":"10.1111/rda.70060","url":null,"abstract":"<p><p>Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone that is expressed in response to stress conditions. HSP90 has been found to be involved in the activation of proteins related to cell division and female reproduction. However, its specific role in porcine oocyte maturation, particularly in cytoskeletal formation, remains unclear. In this study, geldanamycin (GA) was used to inhibit HSP90 activity by binding to its adenosine triphosphate (ATP) binding site. Porcine oocytes surrounded by cumulus cells were cultured in TCM-199 medium for 44 h, with varying concentrations of GA (0.1, 0.5, 1 and 2 μM). It was observed that oocyte maturation significantly decreased when treated with a concentration of 0.5 μM or higher, leading to an increase in oocytes arrested at the germinal vesicle and metaphase I (MI) stage. The expression levels of Cyclin-dependent kinase 1, p-Aurora C (Thr198), p-AKT (Ser473) and p-PLK1 (Thr210) decreased during the MI stage, whereas Polo-like kinase 1 remained consistent with the control group. Additionally, abnormal spindle formation was increased, with abnormalities including aberrant poles, misaligned chromosomes and failure to reach the proximity of the cell membrane. Moreover, examination of mature oocytes at the metaphase II (MII) stage revealed that GA treatment induced a decrease in BCL-2 phosphorylation at the Ser70 site and an increase at the Thr56 site. This led to the release of Cytochrome c from the mitochondria and upregulation of Caspase 3 expression. In conclusion, HSP90 is essential for proper meiotic maturation in porcine oocytes by playing critical roles in meiotic resumption and spindle formation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70060"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA as Biomarkers for Physiological and Stress Processing in the Livestock.","authors":"Montaser Elsayed Ali, Fatimah A Al-Saeed, Ahmed Ezzat Ahmed, Min Gao, Wei Wang, Haimiao Lv, Guohua Hua, Liguo Yang, Mohamed Abdelrahman","doi":"10.1111/rda.70034","DOIUrl":"10.1111/rda.70034","url":null,"abstract":"<p><p>Several microRNAs (miRNAs) have been identified as potential biomarkers widely dispersed in animals since 1993, and they have become a significant molecular biology research area. Because of their ability to activate extracellular molecules, stabilise bodily tissues, control cell-to-cell signals, and be easily extracted, miRNAs are outstandingly nominated as biomarkers. However, there is growing interest in targeting miRNAs to monitor physiological reproductive performance, including reproductive system development, embryo development, fertilisation, endocrinology, and animal welfare in stressful conditions. Moreover, miRNAs play significant roles in gene expression regulation; single miRNAs may have overlapping roles, and on a broader scale, multiple mRNAs govern a single function. Also, miRNAs serve as an intermediary messenger between the environment and reproductive performance, making them a vital component of miRNAs as performance biomarkers under environmental conditions like heat stress. This makes describing a unique miRNA's consequences and functions exceptionally challenging, which may confound many researchers. Also, enhancing our comprehension of miRNAs in response to testicular heat stress could potentially aid in preventing and treating spermatogenesis disorders. Therefore, the present review highlights miRNA's regulatory mechanisms on reproductive performance under heat stress to employ these findings in improving reproduction physiology research.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70034"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Studies in the Endometrium of Fertile and Subfertile Bitches.","authors":"Gail R McRae, Marco A Coutinho da Silva, Erin E Runcan, Julie A Stephens, Christopher Premanandan","doi":"10.1111/rda.70055","DOIUrl":"10.1111/rda.70055","url":null,"abstract":"<p><p>Endometrial biopsies have been used historically as a diagnostic tool as part of breeding management in some domestic species. In the mare, the findings in an endometrial biopsy can relate to the ability of that mare to carry a pregnancy to term. The value of the endometrial biopsy in the canine has not been fully established. In this study, the presence of inflammation, fibrosis and endometrial cysts was characterised in full-thickness uterine biopsies from subfertile bitches in late diestrus/early anestrus and compared to the same findings in fertile bitches. The hypothesis was that subfertile bitches would have more inflammation, fibrosis and endometrial cysts. Full-thickness uterine biopsies were taken at the hysterotomy site at the time of C-section for control cases (n = 103), and previous submissions were collected from The Ohio State Reproductive Service as subfertile samples (n = 263). The cases were blinded, randomised and evaluated. Chi-squared or Fisher's exact tests were used to compare categorical variables between control and experimental samples. The Wilcoxon rank-sum test was used to compare ages between the groups. Overall, inflammation was observed more frequently in the subfertile group in comparison to the control group. Lymphoplasmacytic inflammation was observed more frequently in the subfertile group when compared to the fertile group. The incidence of periglandular fibrosis and endometrial cysts was not significantly different between the two groups. These results indicate that microscopic evaluation of canine uterine biopsies may be more suited to detecting inflammatory changes in the endometrium and isolated endometrial cysts, as well as isolated fibrotic lesions, which may be less impactful on fertility compared to chronic inflammation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70055"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship of Breed and Seminal Quality With Protamination and DNA Integrity of Bovine Spermatozoa.","authors":"Juan Camilo Restrepo, Alfredo Giraldo, Kelly Zapata, Mauricio Rojas, Giovanni Restrepo, Alexandra Usuga","doi":"10.1111/rda.70066","DOIUrl":"https://doi.org/10.1111/rda.70066","url":null,"abstract":"<p><p>During spermatogenesis, most histones are replaced by protamines in the process known as protamination. Protamine deficiency is one of the factors that contribute to DNA instability and damage, which can affect the fertility of bulls. The objective of this study was to evaluate the relationship of breed and seminal quality with the protamination and DNA integrity of bovine sperm. A total of 30 semen samples from five Guzerat bulls and five Blanco Orejinegro (BON) bulls were used. Motility and kinematics were evaluated with a CASA system, morphology by eosin-nigrosin staining and membrane integrity with the HOST test. DNA integrity, viability and protamination deficiency were assessed by flow cytometry (IP/CMA3). Linear models, correlation analysis and comparison of means by Tukey test were performed. The proportion of viable protaminated sperm (CMA3-negative) for BON and Guzerat was 78.9% ± 1.4% and 73.8% ± 3.1%, respectively (p < 0.05). DNA fragmentation was 0.60% ± 0.06% for BON and 0.34% ± 0.04% for Guzerat (p < 0.05) and was negatively correlated with protamination (-0.18, p < 0.01). Positive correlations of protaminated viable spermatozoa with total motility (0.68), progressive motility (0.66), membrane integrity (0.52), rapid sperm (0.71), average path velocity (0.44), linear (0.34) and curvilinear (0.54) velocities were found (p < 0.001). Protamination of bovine sperm correlates with semen quality and is influenced by bull breed.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70066"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Different Cryoprotectant Combinations in Vitrification and Slow Freezing for Ovarian Tissue Preservation in Domestic Cats.","authors":"Rayane Brandão Ribeiro, Aline Queiroz Rodrigues, Beatriz Alves de Aguiar, Jessyca Karoline Oliveira Silva, Marcus Vinicius Rocha de A Carvalho da Costa, Julliene Larissa Dos Santos Bezerra, Yasmin Barboza Ferreira, Ingrid Gracielle Martins da Silva, Tathyana Benetis Piau, Carolina Madeira Lucci, Sônia Nair Báo, Jair Trapé Goulart, Paula Maria Quaglio Bellozi, Fernanda Paulini","doi":"10.1111/rda.70064","DOIUrl":"https://doi.org/10.1111/rda.70064","url":null,"abstract":"<p><p>Over the past decade, increased hunting and habitat disturbance have significantly impacted the endangered population within the Felidae family. Recognising this, it becomes imperative to implement strategies aimed at mitigating this concerning conservation scenario. For this, female fertility preservation is crucial in this context, and studies concerning this field are still scarce. In the realm of cryopreservation, prevalent methods involve slow freezing (SF) and vitrification (V). This study aimed to evaluate various cryoprotective combinations for V or SF processes applied to domestic cat ovarian tissue. Twenty ovaries from 10 healthy cats were dissected, and cortical regions were sectioned into eight fragments measuring 3 mm³ each. These fragments were randomly allocated to three different treatment groups for V (V1, V2 and V3) or SF (SF1, SF2 and SF3). Each group employed solutions with varying concentrations of DMSO, EG and either trehalose or sucrose. The assessment included histological evaluation, follicle counting, immunohistochemical analysis of proliferative activity, and ultrastructural examination. The results demonstrated that the V1 protocol-composed of an equilibration solution with 10% DMSO, 10% EG and 0.1 M trehalose, followed by a V solution with 20% DMSO, 20% EG and 0.1 M trehalose-proved most effective. This combination best preserved follicular morphology, reduced degeneration, supported follicle proliferation and maintained favourable ultrastructural integrity compared to other treatments. These findings provide a valuable foundation for improving fertility preservation in domestic cats, with potential applications for endangered felid conservation programs.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70064"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertility Traits in Japanese Black Cows in Farms Classified by Calving Intervals Using Epidemiological Methods.","authors":"Osuke Suzuki, Mizuho Uematsu, Go Kitahara, Takeshi Osawa, Yosuke Sasaki","doi":"10.1111/rda.70058","DOIUrl":"10.1111/rda.70058","url":null,"abstract":"<p><p>The calving interval (CI) is an important determinant of reproductive performance in Japanese Black cattle. The present study aimed to compare individual fertility traits among farms classified by CI. In total, 49,780 artificial insemination (AI) records for 7327 cows from 2011 to 2014 were obtained from 622 farms located in Miyazaki, Japan. The farms were classified into three groups (referred to as farm CI groups) using the lower and upper 25 percentiles of farm CI as thresholds. The mean CI of individual cows was 403.5 ± 70.9 days. The interquartile ranges of CI for individual cows were 49 days for short CI farms and 114 days for long CI farms. The interval between calving and the first AI was associated with farm CI group and parity (p < 0.05) but not with calving season and gestation length. The conception rate (CR) at the first AI in cows on long CI farms decreased as the interval from calving to the first AI decreased (p < 0.05). Additionally, no differences in CR among farm CI groups were observed for AI number ≥ 4. In long CI farms, cows with two AI treatments had longer interservice intervals than those for AI values of 3 and ≥ 4 (p < 0.05). In summary, fertility characteristics for long CI farms differed from those of farms with short or middle CI, and standard operating procedures need to be improved to decrease the CI.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70058"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}