Reproduction in Domestic Animals最新文献

{"title":"Glycerol Supplementation During Oestrus Synchronisation With Fluorogestone Acetate Affects Follicular Proliferation, Maturation and Apoptosis of Granulosa Cells in Pelibuey Ewes.","authors":"P Escorcia Ortiz, C A Price, A Guzmán Sánchez, H M Guerrero-Netro","doi":"10.1111/rda.70080","DOIUrl":"10.1111/rda.70080","url":null,"abstract":"<p><p>This study investigated the impact of glycerol supplementation on the expression of markers associated with follicular proliferation, maturation and apoptosis in Pelibuey ewes synchronised with long (11 days) and short (5 days) protocols using intravaginal fluorogestone acetate (FGA). Forty-five adult Pelibuey ewes were divided into five groups: control, short protocol with or without glycerol and long protocol with or without glycerol. Follicular dynamics were monitored by ultrasonography, and granulosa cells were collected 15 h after the onset of oestrus to analyse gene expression using RT-qPCR of genes associated with proliferation, maturation, and apoptosis. Results indicated that glycerol supplementation or the duration of the synchronisation protocol influenced the expression of genes related to apoptosis, proliferation and maturation. Specifically, glycerol-treated groups exhibited decreased BCL2 expression, and the ratio BCL2/BAX was lower in all treatment groups compared to the control, particularly in the short protocol plus glycerol. We conclude that the use of glycerol as an ultrashort supplementation during different synchronisation protocols leads to a delay in maturation and induces apoptotic-related pathways in granulosa cells to granulosa cell likely reducing the oocyte viability. Finally, glycerol treatment does not increase the abundance of PCNA associated with granulosa cell proliferation. However, it is observed that short-term treatments promote proliferation compared to the control group.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70080"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12104787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Ram Semen Quality After Supplementation With Gelatin, Agar or Alginate Prior to Cooling Storage\".","authors":"","doi":"10.1111/rda.70063","DOIUrl":"https://doi.org/10.1111/rda.70063","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70063"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-Related Changes in Accessory Sex Organs of Egyptian Jacksassess (Equus asinus): A Morphometric and Histological Study.","authors":"Ahmed Sabry S Abdoon, Afaf A Kishta, Nazema S Abdel-Megeid, Shenming Zeng, Said I Fathalla, Sherif M Shawky, Igor F Canisso, Shimaa R Masoud","doi":"10.1111/rda.70082","DOIUrl":"https://doi.org/10.1111/rda.70082","url":null,"abstract":"<p><p>The accessory sex organs play a crucial role in reproductive function, yet limited research exists on age-related changes in these structures in jacks. In the present work, the accessory sex organs of 60 Egyptian jacks were classified according to their age into young (n = 15), adult (n = 25) and senile (n = 20). Jacks were euthanized and the accessory sex organs were immediately separated and collected. Morphometric measures of the penis, ampulla, seminal vesicles, prostate and bulbourethral gland, including length and weight, were determined. Tissue samples from the ampulla, seminal vesicles, prostate and bulbourethral glands were collected, processed and stained with H&E for histomorphometric examination. Our findings reveal significant age-related alterations in the penial weight and length, the structure and secretory activity of the accessory sex glands, shedding light on the physiological changes that occur with advancing age in this species. Insights gained from this analysis can improve reproductive management practices and contribute to the conservation of donkey populations.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70082"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Oestrus and Hormonal Changes in the Progeny of 2 Genotypes of FecB Gene-Edited Sheep.","authors":"Yang Chulei, Zhang Yiyuan, Tang Hong, Guo Yanhua, Wang Limin, Zhou Ping","doi":"10.1111/rda.70038","DOIUrl":"https://doi.org/10.1111/rda.70038","url":null,"abstract":"<p><p>The FecB gene, the first gene identified for multiple births, can improve reproductive performance in sheep. We used the CRISPR/Cas9 editing system to obtain the FecB gene in Kazakh sheep and mated with unedited Kazakh sheep. After the genotypic screening of the progeny, four B+ and ++ Kazakh ewes, each 2 weeks old and healthy, and close to the same body weight, were selected to study oestrus and hormonal changes in the progeny population of FecB gene-edited Kazakh sheep. Our results showed that the FecB gene was moderately polymorphic (0.25 < PIC < 0.5) in the progeny population, was able to be stably inherited, and showed increased lambing numbers. The number of follicles and tiny follicles differed significantly (p < 0.05) between the 2 genotypes of ewes in the progeny population. Physiologically, FecB gene-edited sheep did not differ from wild-type sheep. Throughout the oestrous cycle, FSH, LH, and E₂ in serum hormones of both sheep genotypes showed variability (p < 0.05) between 72-360 h, 360-528 h, and 12-72 h, respectively, compared to other periods. The concentration of FSH and PROG in follicular fluid was much higher than that of LH and E₂ (p < 0.05). Overall, it is possible to make Kazakh sheep carry the FecB gene by CRISPR/Cas9 editing and stably inherit it into the progeny population. The enhancement of reproductive hormones by the FecB gene is beneficial to follicular development and ovulation and improves the reproductive efficiency of sheep.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70038"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Improves the Development Ability of Sheep Embryos by Reducing ROS During IVEP.","authors":"Hang Zhang, Smhaa Gahallah, Yucheng Liu, Jingjing Wang, Zhenliang Zhang, Xueming Zhao, Pengcheng Wan","doi":"10.1111/rda.70061","DOIUrl":"https://doi.org/10.1111/rda.70061","url":null,"abstract":"<p><p>Sheep in vitro embryo production (IVEP) is of great economic and scientific value, while reactive oxygen species (ROS) can negatively impact the quality and developmental potential of sheep embryos. Therefore, reducing ROS may enhance the efficacy of sheep IVEP. In this study, different concentrations of melatonin (10^-9, 10^-7, 10^-5, 10^-3 M) were supplemented during oocyte in vitro maturation (IVM) and embryo in vitro culture (IVC). The developmental ability of sheep oocytes and embryos, the survival rate of vitrified blastocysts, ROS level, ATP content, ATP synthase activity and related gene expression levels of sheep blastocysts were detected. The results showed that 10^-7 M melatonin significantly improved the developmental ability of sheep oocytes and embryos, elevated the survival rate of blastocysts after vitrification, reduced the ROS level, increased the ATP content and ATP synthase activity and improved the expression levels of quality-related genes in sheep blastocysts. We speculated that melatonin may enhance the developmental competence of sheep oocytes and embryos by reducing the ROS level and promoting ATP synthesis. In addition, high concentrations of melatonin may have an adverse impact on the development of sheep embryos.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70061"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-Citrulline Single Intravenous Administration Promotes Testicular Blood Flow, Plasma Testosterone, Estradiol, Circulating Citrulline and Serum Nitric Oxide Levels in Male Camels Under Heat Stress at the End of Non-Breeding Season.","authors":"Elshymaa A Abdelnaby, Ibrahim A Emam","doi":"10.1111/rda.70079","DOIUrl":"https://doi.org/10.1111/rda.70079","url":null,"abstract":"<p><p>This study aimed for the first time to examine the effects of L-citrulline single administration on the camel testicular haemodynamics, morphometry, steroids (estradiol [E2] and testosterone [T]), nitric oxide (NO) and total antioxidant capacity (TAC) under environmental heat stress conditions. For this purpose, 10 adult Maghateer male camels were examined during September 2024 at the end of the non-breeding season. Animals were categorised into a control group (n = 5) and a treated group that received an intravenous dose of L-citrulline (n = 5;155 μmol/kg BW). Testicular length, volume, coloured area, testicular artery (TA) haemodynamics and hormonal profile were examined along the examination (-1, 0, 2, 4, 24, 48, 72, 96 and 120 h after injection). Results represented that no change occurred in testicular length, but the volume was elevated (p < 0.05) from 4 to 96 h after injection with no interaction between treatment and time, but both TA Doppler indices were declined (p < 0.05) at 4-48 h after injection compared to control. Circulating L-citrulline, NO and E2 levels increased 2 h post-injection until 48 h, then all were declined (p < 0.05) again with a lowering pattern of total antioxidant capacity (TAC; p < 0.05), but T took more time to elevate from 4 h until 72 h after injection. L-citrulline could be safe and act as a vasodilator with elevation of steroids (E2 and T), circulating L-citrulline and nitric oxide. This was accompanied by a declination of TAC as early as possible to 48 h. Further studies are needed to show its detailed action on semen quality to be linked with future fertility.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70079"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Three Combinations of Cryoprotectants and Two Warming Temperatures on Cellular Morphology, Morphometry and Mitochondrial Activity of Vitrified Canine Testicles.","authors":"Juliana de Souza Fernandes, Jéssyka Araújo Noronha, Francisco Denilson Rodrigues Gomes, Bruna Farias Brito, Gisele Karla Sena Guimarães, Herlon Victor Rodrigues Silva, Leda Maria Costa Pereira Bersano, Lúcia Daniel Machado da Silva","doi":"10.1111/rda.70074","DOIUrl":"10.1111/rda.70074","url":null,"abstract":"<p><p>High concentrations of cryoprotectants required for testicular vitrification result in a toxic environment for cells. To mitigate this issue, a suitable alternative is to combine cryoprotectants. The temperature for warming a vitrified sample is also important to assure cell viability. Thus, the aim of this work was to evaluate how combining cryoprotectants (ethylene glycol-EG, glycerol-GLY, and dimethyl sulfoxide-DMSO) in pairs and using two warming temperatures (37°C and 50°C) influence cellular morphology, tubular morphometry, and mitochondrial activity after testicular vitrification of dogs. Testicular fragments from ten adult dogs were distributed among the fresh control group (CTR) and the experimental groups according to the combination of cryoprotectants and temperatures (EG/GLY37, EG/GLY50, DMSO/GLY37, DMSO/GLY50, DMSO/EG37 and DMSO/EG50). The fragments were vitrified in a final concentration of 5.6 mol/L (2.8 mol/L of each of the cryoprotectants combined two by two) and subsequently warmed up to 37°C/30 s or 50°C/5 s. Following this, they were processed for histomorphological, morphometric, and mitochondrial activity evaluations with Rhodamine 123. In the morphometric evaluation, all vitrified groups showed a significant reduction in tubular diameter (p < 0.05). All experimental groups showed greater basement membrane separation when related to the CTR (p < 0.05). DMSO/EG37 showed the greatest basement membrane separation when compared to all other groups (p < 0.05). Regarding membrane retraction, all vitrified groups, regardless of the warming temperature, had greater retraction when related to CTR (p < 0.05), except DMSO/GLY50, which did not differ from any group (p > 0.05). Regarding the distinction between spermatogonia and Sertoli cells, no groups warmed up to 50°C differed from the control, except DMSO/GLY37. For nuclear visualisation, none of the vitrified groups differed from the CTR (p > 0.05), except DMSO/GLY37 (p < 0.05), which showed better nuclear visualisation. For the nuclear condensation parameter, there were no significant differences among the groups (p > 0.05). Mitochondrial activity was reduced in all vitrified samples, regardless of the combination of cryoprotectants and warming temperature (p > 0.05). It was concluded that the association of DMSO/GLY50 presented better preservation of morphological aspects.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 5","pages":"e70074"},"PeriodicalIF":1.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Ionised Calcium and Ionised Magnesium in Dairy Cows and Their Associations With the Incidence and Severity of Postpartum Metritis.","authors":"Eduard Jose-Cunilleras, José Angel Robles-Guirado, José Ríos, Guillermo Elcoso, Alex Bach, Anna Bassols","doi":"10.1111/rda.70057","DOIUrl":"10.1111/rda.70057","url":null,"abstract":"<p><p>Peripartum is a critical period for dairy cows during which they may undergo postpartum diseases such as hypocalcaemia and metritis. Determination of serum total calcium (tCa) is widely used to monitor imbalances in this period, but determination of total magnesium (tMg) and ionised forms of both cations (iCa and iMg) has been less studied. In the present work, 42 healthy cows and 53 cows diagnosed with mild or moderate metritis were included. Serum samples were collected 10 days prepartum and 1, 3 and 10 days postpartum to determine tCa, iCa, tMg and iMg concentrations, as well as their corresponding ratios. Our results show that hypocalcaemia and hypermagnesaemia occur on Day 1 postpartum in cows that stayed healthy and cows undergoing metritis. Cows with moderate metritis showed lower serum concentrations of iCa and iMg throughout the period. Low values of prepartum iCa and iMg at 10 days before calving were associated with later development of metritis and therefore could represent potential prognostic indicators. In conclusion, the determination of iCa, tMg and iMg in serum, in addition to tCa, could offer a more comprehensive view of the ionic status of the periparturient dairy cow and may provide information about the risk of suffering uterine diseases.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70057"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Gestational Age in Warmblood, Standardbred and Thoroughbred Horses Based on the Table Established in Light Breed Horses Using Foetal Ultrasonographic Measurements of Four Biometric Parameters.","authors":"Catherine D Renaudin, Valerie Picandet, Philip H Kass","doi":"10.1111/rda.70059","DOIUrl":"https://doi.org/10.1111/rda.70059","url":null,"abstract":"<p><p>Using the equation of Hartwig et al. and the table established for light breed horses, day of gestation was predicted in 108 Warmblood (WB) and 59 racehorses (Thoroughbred [TB] and Standardbred [STB]) pregnant mares. In WB, gestational ages were predicted within 2 weeks from 100 to 200 days of gestation using biparietal diameter (BPD), aortic diameter (AortD) or femur length (FL); within 2 weeks from 100 to 250 days using BPD or AortD; within 2 weeks from 100 to 300 days using BPD alone; and within 3 weeks from 100 to 350 days using eye approximated volume (EyV) alone. In the racehorse group, gestational ages were predicted within 2 weeks from 100 to 200 days of gestation using BPD or AortD; within 3 weeks from 100 to 250 days of gestation using BPD or FL; and within 3 weeks after 300 days of gestation using EyV. Across breeds including Quarter Horses (QH), WB, STB and TB, BPD was the best foetal age predictor from 100 to 200 days of gestation and from 200 to 300 days of gestation with accuracies within 2 and 3 weeks, respectively. After 300 days of gestation, EyV was found to be the best predictor with an accuracy of within 3 weeks. Overall, in the two groups, the breed of the foal nor the breed of the dam (except for short days increments), had any effect on foetal age prediction. Therefore, the table established for light breed horses can be used to assess the foetal age of foetuses carried by recipient mares whether they are WB or STB. Using the same anatomical landmarks but a different ultrasound machine, both ultrasonographers determined foetal age with similar accuracy.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70059"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Ultrasonic Evaluation of the Corpus Luteum in Alpacas With Embryo Removal on Day 9 Post-Mating\".","authors":"","doi":"10.1111/rda.70056","DOIUrl":"10.1111/rda.70056","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70056"},"PeriodicalIF":1.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}