Reproduction in Domestic Animals最新文献

{"title":"Prevalence and Morphological Appearance of Sperm Nuclear Defects Using Feulgen Reaction in Beef Bulls.","authors":"Carlos E Fernandes, Adriane L Zart, Juan Cuevas de Alvarenga Martins, Richard Oko, Peter Sutovsky, Ériklis Nogueira","doi":"10.1111/rda.70021","DOIUrl":"https://doi.org/10.1111/rda.70021","url":null,"abstract":"<p><p>The objective of this study was to assess the prevalence and compare the morphological aspects of sperm nuclei abnormalities in beef bulls submitted to breeding soundness evaluation (BSE). Semen samples were collected by electroejaculation from 649 beef bulls (Bos indicus, n = 515 and Bos indicus × Bos taurus crossbreed, n = 134) ranging from 2 to 12 years of age. Following a clinical evaluation and semen assessment, a slide was prepared with fresh semen and stained by using the Feulgen-stain for the purpose of evaluating sperm and nuclear morphology. Abnormal sperm nuclei were classified into three categories: A, multiple vacuoles in a pouch-like formation; B, a single vacuole such as the nuclear crater/diadem defect; and C, abnormal chromatin condensation. In accordance with the BSE criteria, the animals were classified as either satisfactory or unsatisfactory potential breeders. The prevalence of nuclear defects was comparable between satisfactory bulls of both genotypes. Unsatisfactory Bos indicus bulls exhibited a higher frequency of A and B nuclear defect categories. A category presented an odds ratio of 7.14 (0.012) for unsatisfactory bulls. A low percentage of the C category (2.3%) was observed in both BSE classifications. Vacuoles or craters were predominantly observed in the post-acrosomal and mid-subacrosomal regions, with minimal occurrence in the apical ridge region. Our findings provide the baseline for nuclear defects in B. indicus and its crossbreeds. The Feulgen reaction thus enables a simple yet comprehensive analysis of nuclear sperm head defects during BSE. In addition, stain retention facilitates a practical evaluation and an accurate account of the nuclear lesion types.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70021"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Enhanced Uterine Involution on Reproductive Performance in Multiparous Ewes.","authors":"Dongliang Zhang, Chenlong Wang, Tingting Zhu, Hong Tao, Lei Liu, Xu Li, Hongli Wang, Guangdong Hu, Yanping Wang, Weibin Zeng","doi":"10.1111/rda.70044","DOIUrl":"https://doi.org/10.1111/rda.70044","url":null,"abstract":"<p><p>Rapid uterus involution is essential for minimising interpartum intervals, enhancing reproductive performance, and optimising production in ewes. This study aimed to accelerate the uterine involution of postpartum ewes through a relatively simple hormone treatment approach. In this study, 96 Dorper × Hu F1 ewes were assigned to three groups. The ewes were subsequently injected with normal saline (control group), prostaglandin + oxytocin (PG + OT group) and prostaglandin + oxytocin + horse chorionic gonadotropin (PG + OT + eCG group). Each group had 16 ewes producing a single lamb and 16 ewes producing twin lambs used to determine the effects of different treatments on their uterine involution and reproductive performance. PG + OT + eCG treatment accelerated the rate of uterine horn involution of single lambs (21.40 ± 0.89 days vs. 30.66 ± 1.03 days, p < 0.05) and twin hlambs (22.2 ± 1.09 days vs. 30.33 ± 0.81 days, p < 0.05) compared to the control group. PG + OT + eCG treatment also accelerated the regression of the uterine wall serosa structure and the removal of uterine effusion compared to PG + OT treatment, whose effect was moderate. PG + OT and PG + OT + eCG treatments had no significant effect on the recovery of the maximum uterine diameter of single and double lambing ewes compared to the control group. However, both treatments shortened the first estrus time of postpartum ewes (53.73 ± 3.69 days vs. 48.06 ± 5.87 days vs. 46.46 ± 7.41 days, p > 0.05). There was no significant difference in the conception rate among groups (p > 0.05). Notably, the change trend of reproductive hormones in postpartum ewes was consistent. PG + OT and PG + OT + eCG treatments significantly increased the concentration of estradiol and follicle-stimulating hormone during uterine involution (p < 0.05), but inhibited the secretion of progesterone compared to the control group. The peak of the luteinising hormone in the two treatment groups appeared 14 days earlier compared to that of the control group. In summary, exogenous PG + OT + eCG increases the concentration of estradiol during uterine involution, inhibits the secretion of progesterone, and accelerates the postpartum uterine involution and postpartum estrus time of ewes. These findings provide a basis for exploring the mechanism of uterine involution in sheep and improving sheep production efficiency.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70044"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Estrus and Double Ovulations on Reproductive Efficiency in Summer, of Holstein Heifers on a 5-Day Hormonal Synchronisation Protocol With and Without Intravaginal Progesterone Device.","authors":"Ángel Revilla-Ruiz, Patricia Carulla, Alejandro Pérez-Navarro, Raquel Patrón-Collantes, Eduardo De Mercado, María de la Luz Pérez-Solana, Juan Vicente González-Martín, Francisco Sebastián, Sonia Pérez-Garnelo, Susana Astiz","doi":"10.1111/rda.70017","DOIUrl":"https://doi.org/10.1111/rda.70017","url":null,"abstract":"<p><p>The influence of estrus and double ovulations of Holstein heifers on a 5d-Co-synch 72 h protocol during summer was explored. Cycling heifers on the 5d-Co-72 h synchronisation protocol were randomised to carry a progesterone device (116) from day 0 to day 5 of the synchronisation protocol or not (97), from them 100 and 84 underwent fixed-time artificial insemination (FTAI). Another 70 heifers showed estrus before the first GnRH and were inseminated. Heifers were pregnant 34% (33/97) without device, 44.0% (51/116) with device and 44.3% (31/70) inseminated after estrus. Pregnancy per insemination (P/AI) tended to be higher (p = 0.059) inseminating after estrus than after FTAI without device. Three heifers without device and two with it showed double ovulation, four of which became pregnant. Pregnancy loss was similar among groups (0%-10%). P/AI was higher in heifers with estrus before FTAI (OR 13.96, p = 0.027), and with each additional day of age (OR 1.015; p = 0.015). The device was associated with higher progesterone on day 5, with more synchronised heifers, more ovulation frequency after first GnRH (96.6% vs. 84%, p = 0.007), and more heifers showing estrus before FTAI (96.0% vs. 85.7%, p = 0.018). Days to conception after first AI of the study, and after maximum three AIs were similar between synchronised groups, but significantly shorter in heifers inseminated after observed estrus. Fertility was higher in heifers that showed signs of estrus before accompanying the FTAI, while double ovulation seems not to be a major factor for fertility. An intravaginal device slightly improves efficiency under heat stress by increasing ovulation, progesterone, proportion of synchronised heifers and heifers showing estrus at AI.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70017"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Time-Lapse Incubation System on In Vitro Development of Alpaca Embryos.","authors":"Cendy Lutz Ccasa Ccahuana, Pedro Walter Bravo Matheus, Ying Liu, Guiulfo Duriel Mamani-Mango","doi":"10.1111/rda.70008","DOIUrl":"https://doi.org/10.1111/rda.70008","url":null,"abstract":"<p><p>Currently, incubators with a time-lapse system are widely used for in vitro embryo production in several species, however, their effect on alpaca embryo development compared to conventional incubators remains unknown. The aim of this study was to compare early in vitro embryo development in alpacas using a time-lapse incubator system versus a conventional incubator. Ovaries were obtained from a slaughterhouse and 1048 cumulus-oocyte complexes (COCs) were collected and in vitro matured for 26 h in either a time-lapse system (n = 542) or a conventional incubator (n = 542). All the matured COCs were fertilised for 24 h with fresh spermatozoa recovered from epididymis obtained from the slaughterhouse. The presumptive zygotes in both systems were cultured in a commercial one-step culture medium, and the embryo development was evaluated at 48, 120 and 168 h after fertilisation. Our results showed no differences in maturation rates between the time-lapse system and the conventional incubator (79.9% vs. 55.0%, p = 0.080). Cleavage rates at 48 h were also similar between the time-lapse and conventional systems: 20.5% vs. 20.8% for embryos at the two- to four-cell stage, and 6.0% vs. 7.0% for embryos above the four-cell stage (p > 0.05). At 120 h after fertilisation, the percentage of embryos were more evenly distributed across all stages (below eight-cell, eight-cell and above eight-cell stages) in both systems (p > 0.05). At 168 h, no differences in the blastocyst rates and blastocyst quality were observed between the time-lapse and conventional systems. In conclusion, in vitro embryo production in alpacas can be successfully carried out using the time-lapse incubation system or a conventional incubator with comparable results. Further study would focus on the correlation between the developmental patterns and the quality of alpaca embryos, as well as the comparison of in vivo embryo development between the two incubation systems.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70008"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Abstracts of the 58th Annual Conference of Physiology and Pathology of Reproduction and 50th Joint Conference of Veterinary and Human Reproductive Medicine, February 26th to 28th 2025, Leipzig.","authors":"","doi":"10.1111/rda.70016","DOIUrl":"https://doi.org/10.1111/rda.70016","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 1 ","pages":"e70016"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen Collection by Urethral Catheterization After Pharmacological Induction With Dexmedetomidine and Methadone in Ferrets.","authors":"Simona Attard, Alice Andolfatto, Marco Di Giuseppe, Maria Carmela Pisu","doi":"10.1111/rda.70009","DOIUrl":"https://doi.org/10.1111/rda.70009","url":null,"abstract":"<p><p>The evaluation of fertility in stud animals is crucial across all species. Various semen collection techniques have been described in the literature, depending on the practitioner's expertise, available equipment and the species being examined. These techniques include the use of an artificial vagina, electroejaculation, urethral catheterization and the administration of alpha-2 adrenergic agonists to induce penile erection and ejaculation. Since 1965, several studies have explored semen collection methods in ferrets. Chang et al. initially performed post-mortem collection from the epididymis and vas deferens, while in 1970, Shump et al. successfully utilised electroejaculation. More recently, reproductive specialists have reported improved semen collection results through urethral catheterization following the administration of alpha-2 adrenergic agonists. The first successful application of this technique in felids was documented in 2008 by Zambelli et al. using medetomidine. In 2017, Pisu et al. employed a similar protocol, combining dexmedetomidine and methadone. In ferrets, urethral catheterization was first described in 2020, using a combination of ketamine, midazolam, and dexmedetomidine. The goal of this study was to present an alternative protocol for semen collection in ferrets, utilising dexmedetomidine and methadone to induce ejaculation.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70009"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review and New Insights Into Spermatogenesis From Single-Cell RNA Sequencing.","authors":"Zhiqiang Wang, Nan Xiao, Qing Han, Yunjia Qi, Deshun Shi, Chan Luo","doi":"10.1111/rda.70019","DOIUrl":"https://doi.org/10.1111/rda.70019","url":null,"abstract":"<p><p>As the most primitive germ cells in the testis, spermatogonial stem cells (SSCs) not only constantly renew themselves to ensure their quantity, but also differentiate into mature sperm cells to complete spermatogenesis and transmit genetic information to the next generation. Successful spermatogenesis is inseparable from niche regulation, which provides factors that enhance the self-renewal of SSCs to maintain their numbers and directs the appropriate differentiation of spermatogonia. Some progress has been achieved in the definition and isolation of SSCs. However, a high degree of cellular heterogeneity is found in the testis, revealing a combination of various cell types at different developmental stages and a lack of specific molecular markers (especially in domestic animals) for fully screening and purifying SSCs. These factors have considerably hindered further research into the mechanisms of maintenance, self-renewal, and differentiation of SSCs, as well as limited their isolation, purification, and applications. Accumulated studies have recently successfully employed single-cell RNA sequencing (scRNA-seq) as a novel approach to detailing the classification of cell subsets, mining specifically expressed genes in different cell types, and accurate identification of specific cell types. This review summarises the progress of SSCs identification and offers new insights into the SSCs developmental trajectory from single-cell RNA sequencing.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70019"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological Changes in the Canine Epididymis During Downregulation and Recovery After Deslorelin Treatment.","authors":"Henriette Greiner, Hanna Körber, Eva-Maria Packeiser, Ines Focks, Selim Aslan, Serhan Ay, Murat Findik, Gülşah Saral, Eser Akal, Christelle Fontaine, Sandra Goericke-Pesch","doi":"10.1111/rda.70013","DOIUrl":"10.1111/rda.70013","url":null,"abstract":"<p><p>Slow-release GnRH agonist implants containing deslorelin (SRI) are registered for temporary suppression of male fertility. The effect of SRI treatment on canine testicular function is well characterised, although the effect of downregulation and subsequent recovery on epididymal function has not been studied yet. Therefore, twenty-nine healthy male dogs were treated with a 4.7 mg SRI for five months. Subsequent to implant removal, groups of 4-5 dogs were surgically castrated either at implant removal (week 0) or 2, 4, 6, or 10 weeks later. Three subgroups were categorised according to pre-surgical testosterone levels. Five healthy untreated dogs served as control. Epididymides were separated into head, body and tail. Epididymal duct diameter and epithelial height were measured using haematoxylin-eosin-stained sections of each dog and part of epididymides. Besides, the presence of spermatozoa, the cilial height, the thickness of the muscle layers and the relative amount of connective tissue were semiquantitatively assessed. The downregulated epididymis was characterised by a reduced epithelial height and epididymal duct diameter, lower cilia and absence of sperm, but more connective tissue, supporting that epididymal function is significantly altered by SRI treatment. At recovery subsequent to implant removal, the histomorphology was comparable with untreated controls. The study indicates that recovery of the epididymal function, like spermatogenesis, depends on testicular testosterone production.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70013"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11815533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Welcome Note to the Participants of the 58th Annual Conference of Physiology and Pathology of Reproduction and 50th Joint Conference of Veterinary and Human Reproductive Medicine (February 26-28, 2025, Leipzig).","authors":"","doi":"10.1111/rda.70015","DOIUrl":"https://doi.org/10.1111/rda.70015","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 1 ","pages":"e70015"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Müllerian Hormone Concentrations Can Be Reliably Determined by a Single Measurement, Irrespective of Cycle, in Synchronised Ewes During Non-Breeding Season.","authors":"Nebi Cetin, Davut Koca","doi":"10.1111/rda.70010","DOIUrl":"10.1111/rda.70010","url":null,"abstract":"<p><p>Improvement of yield characteristics in animal breeding is important in terms of increasing animal production and sustainability. Fertility is one of the most important yield traits affecting economic gain in sheep breeding. Anti-Müllerian Hormone (AMH) is widely recognised as a dependable biomarker for assessing ovarian reserves and fertility potential. The aim of this study was to evaluate the dynamics of AMH during different phases of the sexual cycle in Norduz ewes the non-breeding season. Additionally, the study sought to assess the effects of age and body condition score (BCS) on AMH concentrations during these phases. A total of 32 Norduz ewes with a body condition score (BCS) of 3-4.5 and aged between 2 and 4 years were used as animal material in the study. All experimental procedures were carried out outside the breeding season and when the ewes were lactating. In all ewes in anestrus, intravaginal sponges (Esponjavet, 60 mg MAP, Hipra, Turkey) were kept in the vagina for 7 days for estrus synchronisation. Intramuscular injections of PMSG (Oviser, 500 IU, Hipra, Turkey) and PGF2α analog (Gestavet, 50 μg, Hipra, Turkey) were administered 48 h prior to sponge removal. Twenty-four hours after sponge removal, ewes were exposed to the ram for estrus detection. Since 5 ewes did not show estrus, blood samples were collected regularly from animals (n = 27) in which estrus was detected at three different stages: one just before the insertion of vaginal sponges (anestrus), another when heat was detected exposing to the ram (estrus), and the final one 10 days after estrus (diestrus). The serum samples were assessed for the levels of AMH and progesterone through the electrochemiluminessence immunoassay technique (ECLIA). The results of the analyses showed that serum AMH concentration did not vary between anestrus, estrus and diestrus phases of the sexual cycle of Norduz ewes outside the breeding season (p > 0.05). Furthermore, age and BCS had no effect on progesterone and AMH levels in different phases of the sexual cycle (p > 0.05). In conclusion, this study shows that serum AMH levels are constant at any stage of the estrus cycle. This suggests that phenotypic evaluation of ewes can be performed with a single measurement and that AMH is a reproducible and dependable biomarker that can be measured at any stage of the estrus cycle at an arbitrary time point.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 2","pages":"e70010"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}