Reproduction in Domestic Animals最新文献_第2页

Editorial. 社论。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70101

Kalin Hristov, Elena Kistanova

引用次数: 0

Effect of Kisspeptin Fortification in Freezing Media on Post-Thaw Quality and Fertility of Buffalo Bull Semen. 在冷冻培养基中添加Kisspeptin对水牛精液解冻后品质和育性的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70124

Balkar Singh, Ashwani Kumar Singh, Pooja Devi, Ajeet Kumar, Narinder Singh, Navdeep Singh Ratta, Prahlad Singh

{"title":"Effect of Kisspeptin Fortification in Freezing Media on Post-Thaw Quality and Fertility of Buffalo Bull Semen.","authors":"Balkar Singh, Ashwani Kumar Singh, Pooja Devi, Ajeet Kumar, Narinder Singh, Navdeep Singh Ratta, Prahlad Singh","doi":"10.1111/rda.70124","DOIUrl":"https://doi.org/10.1111/rda.70124","url":null,"abstract":"The present study was undertaken to assess the effect of kisspeptin supplementation (0.0, 5.0, 10.0, 15.0, 20.0 and 40.0 μM) in cryodiluent on freezability and in vivo fertility of buffalo bull spermatozoa. Twenty-four semen ejaculates were collected using an artificial vagina from four Murrah buffalo bulls. Ejaculates having > 70% sperm motility, < 25% morphological abnormalities and > 500 million/ml concentration were diluted with Tris-egg yolk extender containing 0.0 (control), 5.0, 10.0, 15.0, 20.0 and 40.0 μM kisspeptin and cryopreserved following established protocol. The CASA-based progressive motility and viability were higher (p < 0.05) in cryodiluent containing 20.0 μM of kisspeptin in comparison to other experimental concentrations and control. Addition of kisspeptin at 20.0 and 40.0 μM in extender exhibited higher (p < 0.05) CASA-based total motility and plasma membrane integrity. Regarding CASA-based sperm kinematics, supplementation of kisspeptin (20.0 μM) in extender improved (p < 0.05) VCL and VAP as compared to other tested concentrations and control. The acrosome integrity revealed no difference (p > 0.05) in all the experimental extenders compared to control. The MDA levels were lower (p < 0.05) in cryodiluent supplemented with kisspeptin (20.0 μM) than in other concentrations and control. Sixty artificial inseminations were performed with the post-thaw semen having the most effective dose of kisspeptin (20.0 μM) and control (30 inseminations each). The conception rates were recorded to be higher (p > 0.05) using post-thaw semen containing kisspeptin (20.0 μM; 43.3%) as compared to control (33.3%). In conclusion, complementing the cryodiluent with 20.0 μM kisspeptin improved the quality and in vivo fertility of cryopreserved Murrah buffalo bull semen.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70124"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Full-Term In Vivo Development of Frozen-Thawed Mammalian Diapausing Embryos. 哺乳动物冻融滞育胚胎的足月体内发育。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70123

Sergei Amstislavsky, Irina Rozhkova, Tamara Rakhmanova, Varvara Kozeneva, Eugeny Brusentsev

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Effects of Donor Cell Type on Developmental Competence of Canine-Porcine Interspecies Cloned Embryos. 供体细胞类型对犬猪种间克隆胚胎发育能力的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70121

Yanxia Yan, Zheng Li, Guke Zhang, Yazheng Dong, Zhenfang Wu, Hao Wang, Zicong Li, Zheng Xu

{"title":"Effects of Donor Cell Type on Developmental Competence of Canine-Porcine Interspecies Cloned Embryos.","authors":"Yanxia Yan, Zheng Li, Guke Zhang, Yazheng Dong, Zhenfang Wu, Hao Wang, Zicong Li, Zheng Xu","doi":"10.1111/rda.70121","DOIUrl":"10.1111/rda.70121","url":null,"abstract":"Canine somatic cell nuclear transfer (SCNT) is a powerful technology that can be used to clone beloved companion dogs, produce valuable working dogs, rescue endangered canine breeds, and create genetically engineered dogs. Nevertheless, the application of this technology is hindered by the low developmental efficiency of canine SCNT embryos. It has been shown that in pig and horse cloning using mesenchymal stem cells (MSCs), compared with fibroblasts, as donor cells can enhance the developmental potential of SCNT embryos. Whether this is also true for dog SCNT is unknown. This study aims to evaluate the efficiency of MSCs and fibroblasts as donor cells for canine SCNT. We demonstrated that canine adipose tissue-derived MSCs (cAd-MSCs) have a higher growth rate, lower apoptosis level, and higher expression level of pluripotency genes and HOX family genes than canine ear fibroblasts (cEFs). These features enhance the reprogramming efficiency of cAd-MSCs in canine-porcine interspecies SCNT (iSCNT) embryos, resulting in a higher developmental competence in cAd-MSCs-derived iSCNT embryos than that in cEFs-generated counterparts. This study indicates that cAd-MSC is a better donor cell type than cEF for SCNT.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70121"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen. 化学定义的种马精液液体贮存长期延长剂的评价。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70126

Leonardo F C Brito, Renata L Linardi, Leslie A S Rosales, Nithiya Sri Balamurugan, Camilo Hernández-Avilés, Luisa Ramírez-Agámez

{"title":"Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen.","authors":"Leonardo F C Brito, Renata L Linardi, Leslie A S Rosales, Nithiya Sri Balamurugan, Camilo Hernández-Avilés, Luisa Ramírez-Agámez","doi":"10.1111/rda.70126","DOIUrl":"10.1111/rda.70126","url":null,"abstract":"Efficient use of stallion semen in liquid state is limited by its relatively short shelf-life. A chemically defined extender (Beyond) is now available for long-term liquid semen preservation. The objectives of the present study were to compare Beyond with milk extenders for the preservation of semen at two temperatures, and to evaluate fertility of semen cooled for 4-8 days before artificial insemination. Semen was processed using different extenders: milk, cholesterol (BotuSemen Special); milk-based (INRA 96); and Beyond. Sperm motility, membrane and acrosome integrity, and chromatin structure were evaluated in semen stored at 17°C for 7 days or at 5°C for 14 days. Sperm motility decreased in the first few days of storage regardless of extender or storage temperature. Sperm motility continued to decline at relatively constant rates in semen extended in milk extenders, but the rate of decline was substantially reduced with Beyond. Sperm motility in semen extended with Beyond was greater than in semen extended with milk extenders after 4 days of storage at 17°C, or after 7 days of storage at 5°C. Extender did not affect sperm DNA damage during storage, but sperm with intact membrane and intact acrosome were lower with Beyond. Inseminations with semen stored with Beyond at 5°C for an average of 5.5 days resulted in embryos in 61% of cycles (11/18). In conclusion, Beyond extender resulted in greater sperm motility longevity when compared to milk extenders, especially when semen was stored at 5°C. Satisfactory fertility was obtained with semen cooled for 4-8 days before artificial insemination.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70126"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Issue: Proceedings of the 28th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) and 3rd European College of Animal Reproduction (ECAR) symposium, 11-13 September 2025, Albena Resort, Bulgaria. 特刊：第28届欧洲家养动物繁殖学会年会（ESDAR）和第三届欧洲动物繁殖学院（ECAR）研讨会论文集，2025年9月11日至13日，保加利亚阿尔贝纳度假村。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70096

引用次数: 0

Special Issue: Proceedings of the 28th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) and 3rd European College of Animal Reproduction (ECAR) symposium, 11-13 September 2025, Albena Resort, Bulgaria. 特刊：第28届欧洲家养动物繁殖学会年会（ESDAR）和第三届欧洲动物繁殖学院（ECAR）研讨会论文集，2025年9月11日至13日，保加利亚阿尔贝纳度假村。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70097

引用次数: 0

Special Issue: Proceedings of the 28th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) and 3rd European College of Animal Reproduction (ECAR) symposium, 11-13 September 2025, Albena Resort, Bulgaria. 特刊：第28届欧洲家养动物繁殖学会年会（ESDAR）和第三届欧洲动物繁殖学院（ECAR）研讨会论文集，2025年9月11日至13日，保加利亚阿尔贝纳度假村。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70099

引用次数: 0

Special Issue: Proceedings of the 28th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) and 3rd European College of Animal Reproduction (ECAR) symposium, 11-13 September 2025, Albena Resort, Bulgaria. 特刊：第28届欧洲家养动物繁殖学会年会（ESDAR）和第三届欧洲动物繁殖学院（ECAR）研讨会论文集，2025年9月11日至13日，保加利亚阿尔贝纳度假村。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70098

引用次数: 0

N-Acetylcysteine Promotes the Maturation of Sheep Oocytes and Embryo Development In Vitro. n -乙酰半胱氨酸促进绵羊卵母细胞体外成熟和胚胎发育。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70084

Can Li, Yingbing Zhang, Yifan Pan, Hong Wu, Chengtu Zhang, Ying Wu, Ru Meng, Jianmin Su

{"title":"N-Acetylcysteine Promotes the Maturation of Sheep Oocytes and Embryo Development In Vitro.","authors":"Can Li, Yingbing Zhang, Yifan Pan, Hong Wu, Chengtu Zhang, Ying Wu, Ru Meng, Jianmin Su","doi":"10.1111/rda.70084","DOIUrl":"10.1111/rda.70084","url":null,"abstract":"During the in vitro maturation process of oocytes, oxidative stress is commonly present, and excessive oxidative stress can affect oocyte maturation. Thus, adding antioxidants during maturation is an effective strategy for reducing oxidative stress. N-acetylcysteine (NAC), a derivative of cysteine, participates in glutathione (GSH) metabolism and stimulates glutathione synthesis. However, a clear understanding of the effect of NAC on sheep oocytes remains unknown. In this study, we investigated NAC's impact on the maturation of sheep oocytes, and the results revealed that the maturation rate, and subsequently the cleavage and blastocyst formation, were significantly enhanced by incubation with 1 mM NAC. The GSH and Ca2+ levels increased, and the cortical granules were significantly elevated, whereas the reactive oxygen species levels were significantly reduced in the 1 mM NAC-treated group. Additionally, the number of inner cell masses was significantly increased. The findings of this study support the hypothesis that NAC increases oocyte maturation rate by protecting them from oxidative stress damage. These discoveries provide a new approach for improving the efficiency of in vitro production of sheep embryos.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70084"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0