Reproduction in Domestic Animals最新文献_第2页

Luteal Function and Pregnancy Rate in Bos taurus Heifers Treated With GnRH Prior to Timed Artificial Insemination.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70025

Julia Nobre Blank Camozzato, Bruna Favretto de Souza, João Marcello Rodrigues, Alinne Machado Petrarca Léo, Luiz Francisco Machado Pfeifer, Marcelo Bertolini, Monique Tomazele Rovani, Rafael Gianella Mondadori, Bernardo Garziera Gasperin, Fernando Caetano de Oliveira

{"title":"Luteal Function and Pregnancy Rate in Bos taurus Heifers Treated With GnRH Prior to Timed Artificial Insemination.","authors":"Julia Nobre Blank Camozzato, Bruna Favretto de Souza, João Marcello Rodrigues, Alinne Machado Petrarca Léo, Luiz Francisco Machado Pfeifer, Marcelo Bertolini, Monique Tomazele Rovani, Rafael Gianella Mondadori, Bernardo Garziera Gasperin, Fernando Caetano de Oliveira","doi":"10.1111/rda.70025","DOIUrl":"https://doi.org/10.1111/rda.70025","url":null,"abstract":"The study aimed to evaluate the effects of GnRH given 34 h after progesterone (P4) intravaginal device (IVD) removal in a timed artificial insemination (TAI) protocol. In Experiment 1, 17 Hereford and Braford heifers (control, n = 7; GnRH34; n = 10) received 2 mg estradiol benzoate (EB) and a 1 g P4 IVD on Day 0 (D0). On D8, IVDs were removed and 150 μg d-cloprostenol (PGF) and 1 mg estradiol cypionate (EC) were administered. In the GnRH34 group, animals received 25 μg lecirelin 34 h after the IVD removal (D9). TAI was performed in both groups on D10 (48 h after IVD removal). Follicular dynamics were evaluated from D8 until ovulation; blood samples were collected 7 and 12 days after TAI. In Experiment 2, to evaluate the effect of GnRH34 on pregnancy rates (control, n = 187; GnRH34; n = 203), heifers were subjected to the TAI protocols described in Experiment 1. No significant differences were observed in preovulatory follicle (POF) diameter on D10, follicular growth and ovulation rates until 72 h and P4 concentrations on days 7 and 12 after TAI (p > 0.05). Pregnancy rates (PR) were similar between groups (Control = 50.2%; GnRH34 = 50.2%; p = 0.65). A significant effect of body weight (BW) and body condition score (BCS) on PR was observed for control (p< 0.05), but not for GnRH34. In conclusion, GnRH 34 h after the IVD removal in beef heifers previously treated with EC had no effect on luteal function and fertility.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70025"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Insights in Bovine Follicular Cysts.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70048

Alice Carbonari, Nicola Antonio Martino, Matteo Burgio, Vincenzo Cicirelli, Lorenza Frattina, Maria Elena Dell'Aquila, Annalisa Rizzo

{"title":"New Insights in Bovine Follicular Cysts.","authors":"Alice Carbonari, Nicola Antonio Martino, Matteo Burgio, Vincenzo Cicirelli, Lorenza Frattina, Maria Elena Dell'Aquila, Annalisa Rizzo","doi":"10.1111/rda.70048","DOIUrl":"10.1111/rda.70048","url":null,"abstract":"The aim of the present study was to understand the involved factors in follicular cysts in dairy cows. The study consisted of an in vivo and in vitro approach. The in vivo part, hormonal evaluation (Kisspeptin-10 [Kp-10], Gonadotropin inhibiting hormone [GnIH], Luteinizing hormone [LH], Oestrogens [E2] and cortisol) was performed in sera of both healthy (H) and cows with follicular cysts (FC). The in vitro part was concentrated on estimating the distribution of GPR54 (Kp-10 receptor) and GPR147 (GnIH receptor) on cystic and preovulatory follicles. Serum concentrations of Kp-10, GnIH, LH, E2 and cortisol were significantly higher in Group FC compared with Group H. Gene expression analysis showed a reduction in GPR54 mRNA levels in FC compared to preovulatory follicles, while no expression of the GPR147 receptor was detected. The lower presence of GPR54 in FC compared to the preovulatory follicle can be determined by a down-receptor regulation induced by elevated serum concentrations of Kp-10 in cows with ovarian FC. Endocrine imbalances of the hypothalamic-pituitary-ovarian axis, characterising FC, may reflect altered patterns of Kp-10 and GnIH secretion.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70048"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and Localisation of Aquaporins 1, 7 and 11 in Fresh and Frozen-Thawed Canine Epididymal Spermatozoa.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70051

C A Rojas Zamora, Paris Pizarro, R L De La Sota, M A Stornelli

引用次数: 0

Ovarian Dynamics and Pathological Conditions in Camelids.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70054

Eduardo Arroyo, Nayara Laquiz-Silva

{"title":"Ovarian Dynamics and Pathological Conditions in Camelids.","authors":"Eduardo Arroyo, Nayara Laquiz-Silva","doi":"10.1111/rda.70054","DOIUrl":"https://doi.org/10.1111/rda.70054","url":null,"abstract":"Domestic camelids comprise Dromedary, Bactrian (old-world camelids, OWC), alpaca, and Lama (new-world camelids, NWC). They possess unique reproductive physiology distinct from other livestock. A key feature is being induced ovulators, triggered by beta nerve growth factor (βNGF) present in the seminal plasma. Proper ovarian function is crucial for reproductive management and biotechnologies. In the absence of ovulation, camelids display overlapping follicular waves, with puberty and sexual receptivity influenced by factors like weight and age. Follicular growth patterns vary among these species and are better monitored via transrectal ultrasonography. Physiological variations in ovarian dynamics and hormonal activity differ among members of the Camelidae. Understanding these differences is essential for production and conservation efforts, particularly in wild species. Ovarian disorders, including failure to ovulate or respond to therapy, frequently cause subfertility or infertility in affected females. Superstimulatory and synchronisation protocols that rely on exogenous progestin, eCG, and FSH supplementation have been developed to address some causes of irregular ovarian dynamics. This paper reviews the current knowledge on follicular wave dynamics, their manipulation, and the common ovarian disorders affecting reproduction in camelids.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70054"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Staining Methods to Compare Chromatin Condensation in Fresh and Freeze-Thawed Dog Semen.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70023

Elisa-Marie Laux, Abbas Farshad, Axel Wehrend, Mohamad Eid Hammadeh

{"title":"Application of Staining Methods to Compare Chromatin Condensation in Fresh and Freeze-Thawed Dog Semen.","authors":"Elisa-Marie Laux, Abbas Farshad, Axel Wehrend, Mohamad Eid Hammadeh","doi":"10.1111/rda.70023","DOIUrl":"10.1111/rda.70023","url":null,"abstract":"Various staining techniques have been used for canine sperm analysis, but direct comparisons using identical semen samples are lacking. This study aimed to assess the efficiency, time requirements and cost-effectiveness of different staining techniques: aniline blue, toluidine blue, acridine orange (AcO), chromomycin A3 (CMA3) and terminal deoxynucleotidyl transferase d-UTP nick-end labeling (TUNEL). Forty semen samples (20 fresh and 20 frozen-thawed) were used to assess chromatin condensation. Significant differences (p < 0.01) were found using two-factor repeated measures variance analysis. Aniline blue staining differed significantly (p < 0.01) from toluidine blue, AcO and CMA3 staining. A significant difference (p < 0.05) was observed between AcO and TUNEL staining for fresh sperm, with no significant differences between TUNEL and other methods. Correlations in fresh sperm samples showed r = 0.567 between AcO and aniline blue, r = 0.645 between AcO and CMA3, and correlations of 0.455 and 0.557 for aniline blue - toluidine blue and aniline blue - TUNEL, respectively. For frozen samples, significant differences were found between aniline blue and toluidine blue and AcO tests (p < 0.05), and between CMA3 and TUNEL staining (p < 0.01). Correlations in frozen samples showed r = 0.582 between AcO and aniline blue, r = 0.752 between AcO and CMA3, r = 0.698 between toluidine blue and aniline blue, and r = 0.536 between CMA3 and aniline blue. A minimal association was found between standard semen analysis and chromatin analysis. In conclusion, toluidine blue is effective for light microscopy staining, while CMA3 is recommended for fluorescence microscopy due to its simplicity, rapidity and cost-effectiveness.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70023"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thiamethoxam Exposure Impairs Oocyte Maturation via Induction of Lipid Metabolism and Mitochondrial Dysfunctions in Porcine.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70020

ChaoRui Wang, HuiLin Peng, XiuWen Yuan, HeWei Ji, YongNan Xu, Nam-Hyung Kim, YingHua Li

引用次数: 0

Identifying Key Genetic Factors Influencing Reproductive Performance in Dairy and Meat Sheep Breeds of the Mediterranean Region.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70039

Maria Consuelo Mura, Giovanni Cosso, Mortadha Ouadday, Chadi Hosri, Joze Starič, Michella Nehme, Vincenzo Carcangiu, Sebastiano Luridiana

{"title":"Identifying Key Genetic Factors Influencing Reproductive Performance in Dairy and Meat Sheep Breeds of the Mediterranean Region.","authors":"Maria Consuelo Mura, Giovanni Cosso, Mortadha Ouadday, Chadi Hosri, Joze Starič, Michella Nehme, Vincenzo Carcangiu, Sebastiano Luridiana","doi":"10.1111/rda.70039","DOIUrl":"10.1111/rda.70039","url":null,"abstract":"This study aimed to detect genetic variations in key genes (BMPR1B, BMP15 and GDF9) that influence crucial reproductive traits, such as lambing rate and litter size in sheep. Understanding the genetic basis of these traits is essential for developing more efficient breeding strategies and selecting superior breeding stock, ultimately contributing to improved reproductive efficiency across different sheep breeds, production purposes and farming systems. We examined polymorphisms in the above genes across 500 adult ewes from three Mediterranean countries. The studied breeds included high-yielding dairy (Sarda), dual-purpose with a meat focus (Awassi) and medium-to-low dairy and meat breeds (Slovenian Bovška, Istrian Pramenka and Jezersko-Solčavska). Genetic variations were analysed and correlated with reproductive efficiency, including the interval in days from ram introduction to lambing based on lambing dates. This was used to assess the potential influence of these genes on follicular maturation and oocyte developmental competence. The analysis identified 15 polymorphisms distributed differently among the breeds, with the Sarda exhibiting 11 SNPs and the Awassi 13. The Slovenian breeds showed distinct genetic variation patterns potentially linked to their production goals. However, no significant association was observed between the identified polymorphisms and the analysed reproductive traits. Further studies on other genes regulating bone morphogenetic protein expression, such as the Gremlin 1 (GREM1) gene, may provide additional insights into the genetic mechanisms underlying reproductive traits.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70039"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nongenomic Progesterone Receptors Mediated Bull Sperm Capacitation and Acrosome Reaction Are cAMP-PKA-Calcium Channel Dependent. 非基因组孕酮受体介导的公牛精子电容和顶体反应依赖于 cAMP-PKA-钙通道。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70045

Vikas Sachan, Atul Saxena, Anuj Kumar, Pratishtha Sharma, Jitendra Kumar Agrawal, Dilip Kumar Swain

{"title":"Nongenomic Progesterone Receptors Mediated Bull Sperm Capacitation and Acrosome Reaction Are cAMP-PKA-Calcium Channel Dependent.","authors":"Vikas Sachan, Atul Saxena, Anuj Kumar, Pratishtha Sharma, Jitendra Kumar Agrawal, Dilip Kumar Swain","doi":"10.1111/rda.70045","DOIUrl":"https://doi.org/10.1111/rda.70045","url":null,"abstract":"Progesterone (P4) secreted from the cumulus cells mediates nongenomic signalling in sperm cells and induces capacitation and acrosome reaction in the oviduct. The induction of these events in bull spermatozoa is not fully understood. Recently, we reported the presence of membrane nongenomic P4 receptors in bull spermatozoa and the involvement of P4-nongenomic signalling through the involvement of complex signalling pathways with cannabinoid receptors in the regulation of bull sperm capacitation and acrosome reaction. Here in this study, using pharmacological agents, we report the involvement of cyclic adenosine 3'5' monophosphate-protein kinase A (cAMP-PKA) and calcium channels (sperm cation channel CatSper-and L-type) in the regulation of P4-dependent nongenomic induction of bull sperm capacitation and acrosome reaction. A total of 24 semen ejaculates were used in the study, and 1 picomolar (pM) and 1 micromolar (μM) P4 concentrations were used for capacitation and acrosome reaction induction, respectively in bull spermatozoa. Further, the P4-receptor blocker mifepristone (20 μM) and several other selective blockers KH7 (cAMP, 3 μM), P9115 (PKA, 10 μM), Nifedipine (L-type, 3 μM) and NNC 55-0396 (CatSper, 3 μM) were used for the exploration of downstream P4-signalling in the regulation of bull sperm capacitation and acrosome reaction. The sperm cells showed 50%-70% inhibition and reduction of P4-response in inducing capacitation and acrosome reaction after selective inhibition of these molecules during the study. Our results from the in vitro experiments in bull sperm cells suggested the involvement of cAMP-PKA, CatSper and L-type calcium channels during P4-induced bull sperm capacitation and acrosome reaction. These results also provided new insights into P4-nongenomic signalling in bull spermatozoa in the regulation of capacitation and acrosome reaction, further strengthening our understanding of these complex events in the oviduct.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70045"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Study of Testicular Hypoxia Adaptation in Tibetan Sheep.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70037

Binyan Yu, Yanan Yang, Yijian Li, Rong Gao, Min Ma, Xinrong Wang

{"title":"Transcriptomic Study of Testicular Hypoxia Adaptation in Tibetan Sheep.","authors":"Binyan Yu, Yanan Yang, Yijian Li, Rong Gao, Min Ma, Xinrong Wang","doi":"10.1111/rda.70037","DOIUrl":"https://doi.org/10.1111/rda.70037","url":null,"abstract":"The Tibetan sheep is a typical hypoxia-tolerant mammal, which lives on the plateau, at an altitude of between 2500 and 5000 m above sea level; the study of its hypoxic adaptation mechanism provides a reference for exploring the hypoxic adaptation mechanism of other animals. To grope for the genetic mechanism of adaptation to the hypoxic environment at the transcriptional level in Tibetan sheep testicular tissue, and to identify candidate genes and key pathways related to sheep adaptation, histological observation of testicular tissues from two sheep breeds was carried out using haematoxylin-eosin (HE) conventional staining. A total of 103 differentially expressed genes (DEGs) were authenticated in high altitude Tibetan sheep (ZYH) and low altitude Tibetan sheep (ZYM) by RNA sequencing technology (RNA-Seq), which included 50 up-regulated genes and 53 down-regulated genes. Functional analyses revealed several terms and pathways that were closely related to testis adaptation to the plateau. Several genes (including GGT5, AGTR2, EDN1, LPAR3, CYP2C19, IGFBP3, APOC3 and PKC1) were remarkably enriched in several pathways and terms, which may impact the Plateau adaptability of sheep by adjusting its reproductive activity and sexual maturation, and protecting Sertoli cells, various spermatocytes, and spermatogenesis processes. The results make a reasonable case for a better understanding of the molecular mechanisms of adaptation to altitude in sheep.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70037"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for Evaluating Testicular Function in Domestic Cats.

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70042

Stepanka Homola, Sait Sendag, Davut Koca, Axel Wehrend

{"title":"Methods for Evaluating Testicular Function in Domestic Cats.","authors":"Stepanka Homola, Sait Sendag, Davut Koca, Axel Wehrend","doi":"10.1111/rda.70042","DOIUrl":"10.1111/rda.70042","url":null,"abstract":"This study aimed to describe the physiological sonomorphology of the cat testicle and to verify the findings by histological and endocrinological analyses. Furthermore, two methods of testicle measurement and volume calculation were compared. For the study, a clinical examination of the testicles was carried out in 39 cats. The testicles were measured with the Podany testimeter and using a sonographic examination. This was followed by a castration and histological examination of the testicle. The testosterone level was measured from a blood sample. The cat testicles showed a characteristic sonomorphology. The parenchyma was homogeneous, with a distinct echogenic mediastinum. The histological examination verified that there was spermatogenesis activity in testicular tissue. It was found that the testosterone concentrations varied greatly between the animals (0.1-4.40 ng/mL) although spermatogenesis was detected in all cats. Significant correlations were detected between right and left testicular volumes of the cats according to the measured values of sonography and Podany testimeter (p < 0.001). In addition, there was a significant and positive correlation between testosterone and testicular volume. Testosterone levels increase with increasing testicular volume according to the sonographic method (p < 0.05). With the help of the sonographic measurement, significantly higher testicular volumes are calculated than were compared with the measurement method with the Podany testimeter (p < 0.05). The present study is the first to provide detailed information and reliable data for the evaluation of the testicle size and volume in male cats as well as for sonomorphology, which can be used as comparison values for the andrological examination of this animal species.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70042"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0