Reproduction in Domestic Animals最新文献_第3页

Oxidative and Mitochondrial Status of Vitrified Equine In Vitro Matured Oocytes Using Temperature-Specific Equilibration Protocols. 使用温度特异性平衡协议的玻璃化马体外成熟卵母细胞的氧化和线粒体状态。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70259

Sonia Gago, Meritxell Vendrell-Flotats, Judith Diaz-Muñoz, Mariví Soriano-Moya, Mònica Acacio, Enric Mestres, Nuno Costa-Borges, Teresa Mogas

{"title":"Oxidative and Mitochondrial Status of Vitrified Equine In Vitro Matured Oocytes Using Temperature-Specific Equilibration Protocols.","authors":"Sonia Gago, Meritxell Vendrell-Flotats, Judith Diaz-Muñoz, Mariví Soriano-Moya, Mònica Acacio, Enric Mestres, Nuno Costa-Borges, Teresa Mogas","doi":"10.1111/rda.70259","DOIUrl":"https://doi.org/10.1111/rda.70259","url":null,"abstract":"Cryopreservation of equine in vitro matured (IVM) oocytes remains a major challenge in assisted reproduction due to their high susceptibility to cryoinjury, including oxidative stress and mitochondrial dysfunction. The aim of this study was to evaluate the physiological status of vitrified/warmed equine IVM oocytes following vitrification using two temperature-specific equilibration protocols by assessing reactive oxygen species (ROS), intracellular glutathione (GSH) content and mitochondrial oxidative activity and distribution patterns. Oocytes were vitrified after 28 h of IVM either at 25°C (in VIT25) or at 38.5°C (in VIT38.5) and cultured for an additional 2 h after warming. No significant differences were detected among groups in ROS levels, GSH content, mitochondrial oxidative activity or distribution patterns (p > 0.05). These results indicate that the vitrification protocols evaluated did not induce detectable alterations in redox balance or mitochondrial function in equine IVM oocytes. This suggests that vitrified/warmed oocytes may maintain characteristics compatible with fertilisation and subsequent embryonic development, although their developmental competence was not directly evaluated and warrants further investigation.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70259"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Dynamics Associated With In Vitro Oocyte Competence in Pigs. 与猪体外卵母细胞能力相关的转录组动力学。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70265

D Gascón-Collado, C G Cabrera-Gomez, P Blanco-Cueto, H Rodriguez-Martinez, C A Martinez-Serrano

{"title":"Transcriptomic Dynamics Associated With In Vitro Oocyte Competence in Pigs.","authors":"D Gascón-Collado, C G Cabrera-Gomez, P Blanco-Cueto, H Rodriguez-Martinez, C A Martinez-Serrano","doi":"10.1111/rda.70265","DOIUrl":"https://doi.org/10.1111/rda.70265","url":null,"abstract":"Porcine in vitro embryo production (pIVP) would have a major impact on both livestock production and biomedical research if its efficiency-currently limited by suboptimal oocyte competence-could be improved. This study aimed to characterize transcriptional dynamics across oocyte in vitro maturation (IVM) and fertilization (IVF). Immature oocytes (IMO), mature oocytes (MO) and zygotes (ZY) produced in vitro were analysed by RNA sequencing followed by differential gene expression and functional enrichment analyses. IMO showed higher abundance of zona pellucida (ZP) transcripts (ZP2, ZP3, ZP4), whereas MO were enriched in genes related to cytoskeletal remodelling and calcium signalling (e.g., ITPR1, TGM2), consistent with the acquisition of cytoplasmic competence. After fertilization, ZY exhibited increased representation of transcripts involved in gamete interaction, vesicle trafficking, and maternal-effect complexes (e.g., IZUMO1R, NLRP5, PADI6), reflecting the transition to post-fertilization regulatory support. The chain of transcriptional shifts defines the molecular progression underlying maturation and fertilization competence in the porcine in vitro system.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70265"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silver Nanoparticles in Donkey Semen Stored at 17°C: Effect on Sperm Parameters and Bacterial Growth. 17°C保存的驴精液中的纳米银：对精子参数和细菌生长的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70282

Verónica Pérez Aguilera, Pilar Vallejo-Soto, Carlos Montero-Palma, Blenda Vilela, Antonio Romero-Salmoral, Ángela Galán-Relaño, Francisco Crespo-Castejón, Antonio González-Ariza, Manuel Hidalgo, Jesús Dorado, Isabel Ortiz, Lidia Gómez-Gascón, Inmaculada Luque

{"title":"Silver Nanoparticles in Donkey Semen Stored at 17°C: Effect on Sperm Parameters and Bacterial Growth.","authors":"Verónica Pérez Aguilera, Pilar Vallejo-Soto, Carlos Montero-Palma, Blenda Vilela, Antonio Romero-Salmoral, Ángela Galán-Relaño, Francisco Crespo-Castejón, Antonio González-Ariza, Manuel Hidalgo, Jesús Dorado, Isabel Ortiz, Lidia Gómez-Gascón, Inmaculada Luque","doi":"10.1111/rda.70282","DOIUrl":"https://doi.org/10.1111/rda.70282","url":null,"abstract":"The aim of this study was to evaluate the effect of silver nanoparticles (AgNPs) on donkey sperm parameters and their antimicrobial efficacy on bacteria isolated in the presence of antibiotics. Nine donkey ejaculates were collected and stored in a skim-milk extender at different AgNPs concentrations (0, 0.01, 0.8, 1.6 and 3.12 mg/mL) at 5°C or 17°C. Sperm parameters, including total and progressive motility (CASA), plasma membrane integrity (PMI) and reactive oxygen species (rROS) production, were evaluated at 0, 24 and 48 h of storage. Additionally, bacteria isolated from donkey semen extended with antibiotics were exposed to AgNPs to determine the minimum inhibitory/bactericidal concentrations (MIC/MBC). Sperm motility parameters were higher at 17°C than 5°C after 48 h. AgNPs showed a dose- and time-dependent cytotoxic effect, with concentrations ≥ 0.8 mg/mL significantly reducing motility, membrane integrity and increasing rROS after 24 h. AgNPs exhibited bactericidal activity at concentrations between 1.56 and ≥ 25 mg/mL. In conclusion, while liquid storage at 17°C is an effective strategy for preserving donkey semen, the use of AgNPs as a standalone antimicrobial is currently limited by their cytotoxicity at effective bactericidal doses.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70282"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-4 Supplementation During In Vitro Culture Modulates the Expression of Stress- and Immune-Related Genes During Porcine Blastocyst Development. 体外培养过程中添加白细胞介素-4调节猪囊胚发育过程中应激和免疫相关基因的表达

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70263

Manuela Garcia-Canovas, Marcos Torregrosa-Fuentes, Adelina Lopez-Jara, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Emilio A Martinez, Maria A Gil, Cristina Cuello

{"title":"Interleukin-4 Supplementation During In Vitro Culture Modulates the Expression of Stress- and Immune-Related Genes During Porcine Blastocyst Development.","authors":"Manuela Garcia-Canovas, Marcos Torregrosa-Fuentes, Adelina Lopez-Jara, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Emilio A Martinez, Maria A Gil, Cristina Cuello","doi":"10.1111/rda.70263","DOIUrl":"https://doi.org/10.1111/rda.70263","url":null,"abstract":"The aim of this study was to evaluate the effect of supplementing in vitro culture medium of porcine zygotes with interleukin-4 (IL-4) on their developmental competence and quality. Cumulus-oocyte complexes (COCs) were matured and fertilized in vitro. The presumptive zygotes were then cultured in vitro for 7 days with different concentrations of IL-4 (25 pg/mL, 100 pg/mL, 25 ng/mL and 100 ng/mL) or without IL-4 (control). Cleavage and blastocyst formation rates were evaluated on days 2 and 7 of culture, respectively. On day 7 of IVC, some control and 25 pg/mL blastocysts were used to assess the expression of a panel of genes related to embryo quality by real-time quantitative PCR (RT-qPCR). The results did not show any positive effect of IL-4 on embryo developmental competence, regardless of its concentration. RT-qPCR revealed a significant downregulation of genes involved in cell cycle regulation (PCNA), DNA damage response and immune pathways (STAT6 and GATA3) in blastocysts treated with IL-4. In conclusion, supplementation of IVC medium with IL-4 did not improve the developmental competence of IVP embryos; however, IL-4 modulated the expression of stress- and immune-related genes in blastocysts.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70263"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Transcriptomic Analysis of In Vitro Matured Oocytes From Cyclic and Prepubertal Gilts Reveals Distinct Molecular Patterns. 周期和青春期前后备母猪体外成熟卵母细胞的比较转录组学分析揭示了不同的分子模式。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70285

Carolina G Cabrera-Gómez, David Gascón-Collado, Paula Blanco-Cueto, Belén Pequeño, Heriberto Rodríguez-Martínez, Cristina A Martínez-Serrano

{"title":"Comparative Transcriptomic Analysis of In Vitro Matured Oocytes From Cyclic and Prepubertal Gilts Reveals Distinct Molecular Patterns.","authors":"Carolina G Cabrera-Gómez, David Gascón-Collado, Paula Blanco-Cueto, Belén Pequeño, Heriberto Rodríguez-Martínez, Cristina A Martínez-Serrano","doi":"10.1111/rda.70285","DOIUrl":"https://doi.org/10.1111/rda.70285","url":null,"abstract":"In vitro embryo production is a key tool in reproductive biotechnology, enabling genetic improvement, basic research on early development, and the generation of biomedical models. Despite high rates of nuclear maturation achieved during in vitro maturation (IVM) of porcine oocytes, inadequate cytoplasmic maturation continues to limit oocyte competence and subsequent development, particularly when using oocytes from prepubertal gilts (PG). The molecular basis underlying differences in oocytes from cyclic females remains incompletely understood. In this study, we performed a comparative transcriptomic analysis of immature (IMO) and in vitro matured oocytes (MO) from cyclic (CG) and PG using RNA sequencing (n = 20/group in 6 replicates). Differential expression analyses (FDR < 0.05) revealed extensive transcriptional changes between groups. The CG group showed enrichment of genes related to MAPK and PI3K-Akt signalling, and extracellular matrix interactions in MO vs. IMO, consistent with a more coordinated and developmentally competent maturation program. In contrast, oocytes from the PG group were enriched in genes related to cell cycle regulation, DNA damage response and homologous recombination, indicating a likely impairment in cytoplasmic maturation. Overall, our results provide complementary insight into the differences in the biological processes underlying IVM of oocytes retrieved from PG or CG in the porcine species.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70285"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Layer-Specific Quantitative Assessment of Collagen Distribution in Equine Uteri Using Masson's Trichrome and Digital Image Analysis: A Comparative Study. 马松三色法和数字图像分析定量评估马子宫胶原分布的层特异性比较研究。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70279

Marta Cerveira-Pinto, Carina Santos, Nicole Gomes, Mário Pinho, Anna Szóstek-Mioduchowska, Ana Amaral

{"title":"Layer-Specific Quantitative Assessment of Collagen Distribution in Equine Uteri Using Masson's Trichrome and Digital Image Analysis: A Comparative Study.","authors":"Marta Cerveira-Pinto, Carina Santos, Nicole Gomes, Mário Pinho, Anna Szóstek-Mioduchowska, Ana Amaral","doi":"10.1111/rda.70279","DOIUrl":"https://doi.org/10.1111/rda.70279","url":null,"abstract":"Equine endometrial fibrosis is a chronic and progressive equine uterine condition characterized by fibrosis that affects mares' fertility, with a wide range of fertility outcomes being reported for these mares. Therefore, assessing layer-specific collagen (COL) distribution is essential for a comprehensive understanding of endometrial remodelling and its impact on equine reproductive potential. Full-thickness uterine samples, including stratum functionalis (SF), stratum basalis (SB) and myometrium, were collected post-mortem from healthy control mares (Category I; n = 10) and those with moderate endometrial fibrosis (Category IIB; n = 10). Samples were analysed using Masson's trichrome and digital imaging. Significant differences between categories were identified exclusively within the superficial SF, where category IIB displayed higher COL area fraction (%Area) and Normalized Integrated Density than healthy controls. When evaluating variations across layers, both groups exhibited a higher %Area and Normalized Integrated Density in the SB compared to the SF, followed by a significant decrease in Normalized Integrated Density towards the myometrium. Furthermore, the Mean Grey Value progressively decreased into the deeper compartments, indicating higher staining intensity, only in the group with moderate fibrosis. In conclusion, layer-dependent COL deposition underscores the value of multiparametric, quantitative approaches for an objective characterization of equine endometrial fibrosis.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70279"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Note to the Editor. 给编辑的注释。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70315

Viv Perry

引用次数: 0

Paternal SIRT1 Inhibition During Capacitation Imprints Histone 4 Hyperacetylation in Mouse Zygotes and Blastocysts. 小鼠受精卵和囊胚获能过程中父本SIRT1抑制导致组蛋白4超乙酰化。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70291

M Iniesta-Cuerda, C Vidal-Garcia, J Nevoral

引用次数: 0

Recent Advances and Emerging Applications in Embryo Vitrification for Livestock. 家畜胚胎玻璃化冷冻技术的最新进展及应用前景。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70269

Cristina Cuello, Marcos Torregrosa-Fuentes, Manuela Garcia-Canovas, Adelina Lopez-Jara, Inmaculada Parrilla, Maria A Gil, Emilio A Martinez

{"title":"Recent Advances and Emerging Applications in Embryo Vitrification for Livestock.","authors":"Cristina Cuello, Marcos Torregrosa-Fuentes, Manuela Garcia-Canovas, Adelina Lopez-Jara, Inmaculada Parrilla, Maria A Gil, Emilio A Martinez","doi":"10.1111/rda.70269","DOIUrl":"https://doi.org/10.1111/rda.70269","url":null,"abstract":"Vitrification has progressively supplanted conventional slow freezing as the method of choice for embryo cryopreservation in both human medicine and animal production, particularly for in vitro-produced embryos and for species or developmental stages highly sensitive to conventional freezing. By combining very high cooling and warming rates with concentrated cryoprotectant solutions and minimal sample volumes, it largely prevents intracellular ice formation and, in experienced hands, consistently yields post-warming survival and developmental rates that exceed those obtained with traditional controlled-rate freezing. In this review, we synthesize recent technical and biological advances in embryo vitrification, with particular attention to cryoprotectant formulations, carrier devices, cooling-rate control and current strategies to enhance cryotolerance. For the main livestock species-cattle, horses, pigs and small ruminants-we outline the present state of this technology, highlighting where vitrification has become routine practice and where adoption remains limited or experimental. Despite the clear progress achieved, implementation in field conditions is still heterogeneous, and truly standardized, species-adapted protocols-ideally amenable to automation and direct transfer-are yet to be fully established.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70269"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Issue: Proceedings of the 18th International Congress of the Spanish Society for Animal Reproduction (AERA), 5 to 7 of November 2026, Granada, Spain. 特刊：西班牙动物繁殖学会（AERA）第18届国际大会论文集，2026年11月5日至7日，西班牙格拉纳达。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70298

引用次数: 0