Reproduction in Domestic Animals最新文献_第3页

Ultrasonographic Assessment of Testicular Biometry and Arterial Blood Flow in Pre- and Postpubertal Miranda Donkeys: Correlations With Semen Quality Parameters. 米兰达驴青春期前后睾丸生物测量和动脉血流的超声波评估：与精液质量参数的相关性

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14727

A Martins-Bessa, M Quaresma, M Novoa, P M Bartlewski, S Silva

{"title":"Ultrasonographic Assessment of Testicular Biometry and Arterial Blood Flow in Pre- and Postpubertal Miranda Donkeys: Correlations With Semen Quality Parameters.","authors":"A Martins-Bessa, M Quaresma, M Novoa, P M Bartlewski, S Silva","doi":"10.1111/rda.14727","DOIUrl":"https://doi.org/10.1111/rda.14727","url":null,"abstract":"The main goals of this study were to document and compare the normal ranges of testicular haemodynamic parameters in pre- (aged 8-12 months; n = 4) and postpubertal (aged 24-259 months; n = 16) Miranda donkeys in the breeding season, and to correlate animal biometric data and testicular Doppler indices with basic semen quality parameters of sexually mature jacks. Colour and pulsed-Doppler ultrasonography were employed to assess blood flow in the left and right distal supratesticular artery (DsTA) and their marginal branches (marginal arteries-MA). Peak systolic velocity (PSV), end diastolic velocity (EDV), pulsatility index (PI) and resistive index (RI) were evaluated in both blood vessels, and TAMV (time-averaged mean velocity), TABF (total arterial blood flow) and TABF rate (TABF-R) were calculated for MA. The mean diameter of MA was greater (p < 0.05; 0.24 ± 0.05 vs. 0.19 ± 0.05 cm; mean ± SD) but TABF-R was less (p < 0.05; 0.004 ± 0.004 vs. 0.02 ± 0.01 mL/s/cm3) in sexually mature donkeys compared with prepubertal animals. Apart from RI values for the right testicle of prepubertal donkeys, PI and RI were consistently greater (p < 0.05) in DsTA compared with MA. Significant correlations were found among select biometric and haemodynamic attributes of the testes (height, width and length, TV, TTV and PSV-ST) and ejaculate characteristics (volume, sperm defects-total, head and midpiece) in sexually mature donkeys (n = 8). The present results highlight the importance of scrotal ultrasonography for the reproductive assessment of jacks and provide reference values, based on the available subpopulation of Miranda donkeys that can be used in their clinical and reproductive management and research, or conservation programmes.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced assisted reproduction technologies in endangered mammalian species. 濒危哺乳动物物种的先进辅助生殖技术。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14700

Thomas Bernd Hildebrandt, Susanne Holtze

{"title":"Advanced assisted reproduction technologies in endangered mammalian species.","authors":"Thomas Bernd Hildebrandt, Susanne Holtze","doi":"10.1111/rda.14700","DOIUrl":"https://doi.org/10.1111/rda.14700","url":null,"abstract":"A new synergistic approach of classical conservation strategies combined with advanced assisted reproduction technologies (aART) allows for protection and rescue of endangered keystone species at the brink of extinction, which can help to safeguard complex ecosystems. Reproduction biology and management in mammal species is not only challenging in regards to their diverging sizes, anatomy, and often unknown physiology; it also requires customized training or chemical restraint protocols for safe handling. Besides these general challenges, there are several new assisted reproduction techniques (ART) specifically tailored to critically endangered mammals. The current portfolio of ART in these mammalian taxa is ranging from sexual cycle characterization and manipulation, semen collection and cryopreservation, artificial insemination, biobanking of living cells, oocyte collection, in vitro fertilization (IVF), and embryo production, embryo transfer as well as stem cell-derived in vitro gametogenesis for generating gametes in culture. The article covers advanced assisted reproduction technologies (aART), success and challenges, as well as ethical implications.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an equine endometrial histology scoring system to complement the Kenney-Doig scale. 开发马子宫内膜组织学评分系统，以补充 Kenney-Doig 量表。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14614

I Martínez-Bartolomé, J Masot, C Serres

引用次数: 0

The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro-produced blastocysts. Cryotop 玻璃化系统可同时冷冻保存大量猪体外生产的囊胚。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14600

A González-Plaza, M Garcia-Canovas, I Parrilla, H Rodriguez-Martinez, M A Gil, E A Martinez, C Cuello

引用次数: 0

Evaluation of Placentome Morphology in the Last Two Periods of Pregnancy in Hair Goats (Capra aegagrus hircus). 评估毛山羊（Capra aegagrus hircus）妊娠最后两个时期的胎盘形态。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14731

Fatma İşbilir, Banu Kandil, İhsan İşbilir, Davut Koca, Barış Can Güzel

{"title":"Evaluation of Placentome Morphology in the Last Two Periods of Pregnancy in Hair Goats (Capra aegagrus hircus).","authors":"Fatma İşbilir, Banu Kandil, İhsan İşbilir, Davut Koca, Barış Can Güzel","doi":"10.1111/rda.14731","DOIUrl":"https://doi.org/10.1111/rda.14731","url":null,"abstract":"This study was carried out on pregnant uteruses obtained from healthy Hair goats (Capra aegagrus hircus). A total of thirteen pregnant uteruses, six second and seven third trimesters, were used. Morphometrically, placentome numbers, lengths, widths and depths were measured. Randomly selected placentomes from the second and third trimesters were stained with the triple staining method. Caruncular and cotyledonary areas, capillary numbers and areas were measured using Qupath v0.5.0 software. The Minitab (version 21.4.1) was used for statistical analysis. While no statistical difference was observed in the number of placentomes between trimesters (p > 0.05), placentome length, width and depth were higher in the third trimester compared to the second trimester (p < 0.001). No difference was observed in the number of caruncular and cotyledonary capillaries in the second trimester. In the second trimester, the caruncular capillary area was higher than the cotyledonary capillary area (p < 0.05). Both caruncular and cotyledonary capillary area parameters were higher in the third trimester than in the second trimester (p < 0.001). In the third trimester, the caruncular capillary area was higher than the cotyledonary capillary area (p < 0.001). The number of caruncular capillaries and cotyledonary capillaries was statistically significantly higher in the third trimester compared to the second trimester. In addition, the number of cotyledonary capillaries was higher than that of caruncular capillaries in the third trimester (p < 0.001). A positive and significant correlation was found between the day of pregnancy and the number of placentomes in the second trimester (p < 0.05). No correlation was observed between the day of pregnancy and the number of placentomes in the third trimester. Vascular area density showed a faster development in foetal tissue than in maternal tissue. Placentome size and angiogenesis increased with the progression of pregnancy.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity. 在解冻扩展剂中加入牛血清白蛋白可改善公猪精子膜和顶体的完整性。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14592

Manuel Álvarez-Rodríguez, Helena Nieto-Cristobal, Eduardo de Mercado

引用次数: 0

Developmental Competence of Somatic Cell Nuclear Transfer Embryos and Interspecies ICSI Zygotes From Bovine Small Antral Follicles. 牛小前卵泡体细胞核移植胚胎和种间 ICSI 子代胚胎的发育能力

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14726

Pham-Truong Duy, Bui Le Quynh Nhu, Pham Quoc Dinh, Cao Hoang Nam, Lam Do Truc Phuong, Dao Quang Tri, Pham Minh Chien, Nhat-Thinh Nguyen, Nguyen Van Thuan, Hong-Thuy Bui

{"title":"Developmental Competence of Somatic Cell Nuclear Transfer Embryos and Interspecies ICSI Zygotes From Bovine Small Antral Follicles.","authors":"Pham-Truong Duy, Bui Le Quynh Nhu, Pham Quoc Dinh, Cao Hoang Nam, Lam Do Truc Phuong, Dao Quang Tri, Pham Minh Chien, Nhat-Thinh Nguyen, Nguyen Van Thuan, Hong-Thuy Bui","doi":"10.1111/rda.14726","DOIUrl":"https://doi.org/10.1111/rda.14726","url":null,"abstract":"Assisted reproductive technologies (ART) play a crucial role in conserving threatened wildlife species such as Bos gaurus. ART requires a large number of mature oocytes, and small antral follicles (SAFs) in the ovary are often used to obtain abundant sources of bovine oocytes. However, oocytes from SAFs often experience difficulty completing maturation and obtaining high quality and quantity of blastocyst formation compared to fully grown oocytes. This study aimed to increase the number of high-quality mature oocytes and improve their potential for ART applications in cloned and interspecies intracytoplasmic sperm injection (ICSI) embryos by utilising L-ascorbic acid (LAA) in pre in vitro maturation (pre-IVM) culture. First, oocytes isolated from SAFs were cultured with the duration of pre-IVM 0, 6, 8, 10 h and different concentrations of LAA to determine good conditions for oocyte maturation. Then, mature oocytes were assessed for their developmental competence through parthenogenesis, cloned and interspecies ICSI embryos. The results showed that 8-h pre-IVM with 50 μg/mL LAA improved the maturation rate and developmental competence of parthenogenetic and clone embryos, especially, improving the high blastocyst quality by increasing cell number and expression of histone acetylation at lysine 9 (H3K9ac). In addition, the culture process improved the nuclear reprogramming of somatic cells after nuclear transfer into mature oocytes, resulting in an increased hatching rate of cloned embryos. It also enhanced the activation and the pronuclear formation rate of Gaurus-Taurus zygotes. Overall, the established pre-IVM culture method enhanced the meiotic and developmental competence of embryos. This procedure opened hope for the preservation of endangered species and other applications.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Method for Liposome-Based Gene Transfer in Cattle Embryos. 基于脂质体的牛胚胎基因转移的高效方法。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14730

Thays Antunes, Ana Beatriz Dos Reis Bartoli, Marcella Pecora Milazzotto

{"title":"Efficient Method for Liposome-Based Gene Transfer in Cattle Embryos.","authors":"Thays Antunes, Ana Beatriz Dos Reis Bartoli, Marcella Pecora Milazzotto","doi":"10.1111/rda.14730","DOIUrl":"https://doi.org/10.1111/rda.14730","url":null,"abstract":"In vitro embryo production (IVP) in cattle is crucial for advancing genetic enhancement and preserving valuable genetic lineages, enabling precise genetic modifications and gene studies through modern techniques. Successful genetic manipulation in cattle embryos requires efficient delivery of exogenous DNA/RNA molecules. This research investigates the efficacy of a single embryo culture system for developing genetically modified zona-free (ZF) embryos and examines the use of liposome-based SAMTOR target siRNA transfer in these individually cultured ZF embryos. The findings indicated that the individual culture system resulted in increased cleavage rates, and blastocyst rates were minimally impacted. The new culture system effectively achieved SAMTOR silencing, with 8-16 cell embryos exhibiting reduction in transcript levels compared to control. Measurement of total protein content in the spent culture media was performed to validate the single-culture approach for further analytical applications. Total protein content analysis demonstrated the system's suitability for comprehensive evaluation of the embryo-media interaction, enhancing the scope for in-depth genetic research and applications. This research sheds light into an innovative method to improve genetic editing techniques in reproduction research.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethical aspects associated with genome alteration techniques applied to animal reproduction research. 与应用于动物繁殖研究的基因组改变技术有关的伦理问题。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14670

Lluis Montoliu

引用次数: 0

Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain. 补编：西班牙动物生殖学会（AERA）第 17 届国际大会论文集，2024 年 10 月 17-19 日，西班牙马德里。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14697

引用次数: 0