Reproduction in Domestic Animals最新文献

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Antibiotic-Free Management of Canine Pyometra With Aglepristone: Endocrine Effects and Post-Treatment Vaginal Hyperplasia. 阿格列司酮治疗犬脓脓症:内分泌影响和治疗后阴道增生。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70127
Thiago Vaz Lopes, Luiz Ernandes Kozicki, Pedro Henrique Lomba de Lima, Natália Siqueira de Lara, Mayara Silvestri, Eduarda Stankiwich Vaz, Rafaela Morettini E Castella, Luciane Maria Laskoski, Fernando Andrade Souza
{"title":"Antibiotic-Free Management of Canine Pyometra With Aglepristone: Endocrine Effects and Post-Treatment Vaginal Hyperplasia.","authors":"Thiago Vaz Lopes, Luiz Ernandes Kozicki, Pedro Henrique Lomba de Lima, Natália Siqueira de Lara, Mayara Silvestri, Eduarda Stankiwich Vaz, Rafaela Morettini E Castella, Luciane Maria Laskoski, Fernando Andrade Souza","doi":"10.1111/rda.70127","DOIUrl":"https://doi.org/10.1111/rda.70127","url":null,"abstract":"<p><p>This study evaluated the clinical efficacy of aglepristone for the management of canine pyometra-either as a sole therapy or in combination with different antibiotic regimens-and investigated the association between aglepristone dosing and the occurrence of vaginal hyperplasia during the subsequent estrous cycle. Eighty-seven intact bitches diagnosed with pyometra were enrolled and divided into two experiments. In Experiment I, 57 bitches were randomly assigned to three treatment groups (n = 19/group): (1) AGL, aglepristone alone; (2) AGL-CEF, aglepristone plus ceftriaxone; and (3) AGL-CEF-MET, aglepristone plus ceftriaxone and metronidazole. Clinical examinations and haematological and biochemical profiles were assessed every 48 h for 15 days. In Experiment II, 30 bitches were treated with aglepristone using two protocols: AGL 4X (10 mg/kg s.c. on Days 0, 1, 8 and 15) or AGL 2X (10 mg/kg s.c. on Days 0 and 1). Incidence and severity of vaginal hyperplasia, as well as post-treatment reproductive performance, were evaluated during the subsequent oestrus. All three treatment protocols achieved clinical resolution of pyometra-including the aglepristone-only group-with normalisation of leukocyte counts and serum biochemical markers. In Experiment II, vaginal hyperplasia occurred in 53.3% of bitches in the AGL 4X group versus 7% in the AGL 2X group. Grade III hyperplasia was associated with pyometra recurrence and reduced pregnancy rates. Overall pregnancy rate in Experiment II was 80%. These findings indicate that aglepristone is an effective, fertility-preserving treatment for canine pyometra, even when administered without systemic antibiotics. However, high-dose or prolonged administration may induce oestrogen-mediated adverse effects such as vaginal hyperplasia and disease recurrence. A reduced dosing regimen appears safer while maintaining equivalent clinical and reproductive efficacy.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70127"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective Role of Curcumin in Ram Sperm Cryopreservation: Evaluation of Spermatological Parameters and PRDX5 Gene Expression. 姜黄素对公羊精子冷冻保存的保护作用:精子学参数和PRDX5基因表达的评价。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70119
Cumali Kaya, Burcu Esin, Melih Akar, Cansu Can, Emre Dünder, Mesut Çevik
{"title":"Protective Role of Curcumin in Ram Sperm Cryopreservation: Evaluation of Spermatological Parameters and PRDX5 Gene Expression.","authors":"Cumali Kaya, Burcu Esin, Melih Akar, Cansu Can, Emre Dünder, Mesut Çevik","doi":"10.1111/rda.70119","DOIUrl":"https://doi.org/10.1111/rda.70119","url":null,"abstract":"<p><p>Cryopreservation of ram sperm is a cornerstone in reproductive biotechnology but is often accompanied by oxidative damage that compromises post-thaw sperm quality. Curcumin, a natural polyphenol with potent antioxidant properties, may offer protection against cryo-induced injuries. This study evaluated the effects of curcumin supplementation at two concentrations (10% and 20%) in semen extenders on the functional and molecular characteristics of ram spermatozoa. A total of 14 ejaculates were collected from healthy adult rams and randomly allocated into three groups: control (no curcumin), C1 (10% curcumin) and C2 (20% curcumin). Semen samples were cryopreserved using a Tris-based extender, and post-thaw evaluations included total motility, viability (eosin-nigrosin), morphology (head, midpiece, tail abnormalities), membrane integrity (HOST), chromatin integrity (toluidine blue) and PRDX5 gene expression via qPCR. Curcumin supplementation, particularly in the C2 group, significantly improved total motility and viability (p < 0.01), while reducing head and tail morphological abnormalities and chromatin damage (p < 0.05), compared to the control. No significant difference was observed in PRDX5 gene expression among groups (p > 0.05). These improvements are likely attributed to curcumin's antioxidant activity, including ROS scavenging and membrane stabilisation. Curcumin supplementation in semen extenders enhances post-thaw sperm quality in rams by improving functional parameters and maintaining chromatin integrity, without modulating PRDX5 gene expression. These findings support the potential of curcumin as a safe and effective cryoprotective additive in ovine artificial insemination and genetic conservation protocols. Further research combining transcriptomic and proteomic analyses (or approaches), along with validation of post-thaw sperm fertility, is needed to clarify curcumin's effects and its potential in livestock breeding.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70119"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beneficial Effects of the Mitochondria-Targeted Antioxidant MitoQ on Bull Semen Post Cryopreservation Quality Characteristics. 线粒体靶向抗氧化剂MitoQ对牛精液冷冻后品质特性的有益影响。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70118
Sara Ataei Nazari, Hossein Zakariapour Bahnamiri, Parisa Yazdanshenas, Pooya Jahandideh-Golroodbari, Manjita Sharma, Eva Tvrda, Hossein Vaseghi Dodaran, Abdollah Mohammadi-Sangcheshmeh, Mohsen Sharafi
{"title":"Beneficial Effects of the Mitochondria-Targeted Antioxidant MitoQ on Bull Semen Post Cryopreservation Quality Characteristics.","authors":"Sara Ataei Nazari, Hossein Zakariapour Bahnamiri, Parisa Yazdanshenas, Pooya Jahandideh-Golroodbari, Manjita Sharma, Eva Tvrda, Hossein Vaseghi Dodaran, Abdollah Mohammadi-Sangcheshmeh, Mohsen Sharafi","doi":"10.1111/rda.70118","DOIUrl":"https://doi.org/10.1111/rda.70118","url":null,"abstract":"<p><p>This experiment evaluated the effects of increasing MitoQ concentrations in semen extender on post-thaw quality of Holstein bull sperm, including motility, membrane integrity, antioxidant status and viability. Semen samples were collected, pooled and diluted with extender containing 0 (control), 5, 50, 500 and 1000 nM of MitoQ and frozen through the standard procedure. An increase in MitoQ supplementation positively influenced total and progressive motility, as well as average path velocity; however, these effects were not statistically significant until the concentration reached 50 nM. The highest MitoQ level (1000 nM) showed no difference from the control group. Supplementation of semen extender with 50 and 500 nM of MitoQ significantly increased sperm membrane integrity and mitochondrial activity. Sperm viability improved significantly in concentrations of 5, 50 and 500 nM of MitoQ compared to control, whereas 1000 nM of MitoQ did not show any difference from the control group. Moreover, MitoQ significantly reduced MDA level regardless of its concentration. The concentrations of 50 and 500 nM of MitoQ significantly reduced ROS concentration. It can be concluded that 50 and 500 nM of MitoQ in extender can improve sperm quality parameters in bull semen.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70118"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Overview of Developmental Disorders Leading to Dystocia in Cattle. 导致牛难产的发育障碍综述。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70083
Jørgen S Agerholm, Cord Drögemüller, David J Steffen, Joana G P Jacinto
{"title":"An Overview of Developmental Disorders Leading to Dystocia in Cattle.","authors":"Jørgen S Agerholm, Cord Drögemüller, David J Steffen, Joana G P Jacinto","doi":"10.1111/rda.70083","DOIUrl":"10.1111/rda.70083","url":null,"abstract":"<p><p>Dystocia due to developmental disorders causing foetal malformation is a significant challenge in bovine obstetrics, often resulting in severe birth complications. This narrative review provides an updated overview of the most common congenital syndromes associated with dystocia in cattle, emphasising both their clinical implications and underlying causes. Congenital disorders such as schistosoma reflexum, perosomus elumbis, arthrogryposis, hydrocephalus, anasarca and embryonic duplication are reviewed in detail, along with their impact on the course of calving. While the management of dystocia due to foetal malformations has remained relatively constant over time-mainly involving assisted delivery, foetotomy or caesarean section-our understanding of their aetiologies has advanced considerably. In addition to environmental factors, such as viral infections during gestation, genetic causes may also be implicated. Genetic aetiologies, including dominant de novo mutations and recessively inherited alleles, such as single nucleotide variants, larger structural variants or aneuploidies, have been identified as the cause of some of these congenital defects. This review provides a comprehensive resource on dystocia due to developmental disorders, offering veterinarians updated knowledge to guide clinical decision making and improve outcomes for both the dam and the calf.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 Suppl 3 ","pages":"e70083"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Pathogenic Leptospira DNA in Cervicovaginal Mucus of Mares With Reproductive Disorders in a Brazilian Herd. 巴西某生殖障碍母马宫颈阴道粘液中致病性钩端螺旋体DNA的检测。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-09-01 DOI: 10.1111/rda.70125
Daiany Motta, Juliana Pedrosa, Walter Lilenbaum
{"title":"Detection of Pathogenic Leptospira DNA in Cervicovaginal Mucus of Mares With Reproductive Disorders in a Brazilian Herd.","authors":"Daiany Motta, Juliana Pedrosa, Walter Lilenbaum","doi":"10.1111/rda.70125","DOIUrl":"10.1111/rda.70125","url":null,"abstract":"<p><p>Leptospirosis is a zoonotic disease caused by pathogenic bacteria of the genus Leptospira. A lesser-known form, equine genital leptospirosis (EGL), has been identified as a chronic and often silent infection involving the colonisation of the mare's genital tract. Despite its potential impact, EGL remains underdiagnosed and poorly understood, particularly in its association with reproductive inefficiency. This study showed the presence of Leptospira spp. DNA by lipL32-PCR in the genital tract of mares with a history of reproductive disturbances. Cervicovaginal mucus samples were collected from 120 adult mares exhibiting recent reproductive problems. Results showed that 30 (25%) of the mares tested positive for Leptospira DNA. Among these 30 positive cases, 23.3% had experienced abortions, 3.3% had stillbirths, 53.3% showed placental alterations, and 36.6% were subfertile. These findings suggest a possible association between EGL and reproductive disorders in mares. The high detection rate of Leptospira DNA in genital samples reinforces the need for increased awareness and improved diagnostic efforts.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 9","pages":"e70125"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arbutin Preserves Boar Sperm During Storage at 17°C by Enhancing the Antioxidant Capacity via the NRF2/GPX4 Signalling. 熊果苷通过NRF2/GPX4信号传导增强抗氧化能力,在17℃条件下保存猪精子。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-08-01 DOI: 10.1111/rda.70103
Wei Liu, Wanying Li, Renjian Lin, Yuren Wang, Peichu Zhou, Weijun Pang, Rongnan Li, Yi Zheng
{"title":"Arbutin Preserves Boar Sperm During Storage at 17°C by Enhancing the Antioxidant Capacity via the NRF2/GPX4 Signalling.","authors":"Wei Liu, Wanying Li, Renjian Lin, Yuren Wang, Peichu Zhou, Weijun Pang, Rongnan Li, Yi Zheng","doi":"10.1111/rda.70103","DOIUrl":"10.1111/rda.70103","url":null,"abstract":"<p><p>Arbutin is a naturally present antioxidant derived from plants. The objective of this study was to examine the effect of arbutin on boar sperm during storage at 17°C and the underlying mechanisms. Our results showed that the addition of arbutin to extenders markedly enhanced the sperm (progressive) motility and plasma membrane and acrosomal integrity on Days 9 and 13 of preservation (p < 0.05), with the most pronounced effect of arbutin at the concentration of 100 μmol/L. The addition of 100 μmol/L arbutin also reduced the level of ROS and elevated the levels of ATP and MMP in boar sperm on Days 9 and 13 of preservation (p < 0.05). The subsequent sperm oxidative damage experiment showed that the addition of 100 μmol/L arbutin significantly alleviated the decrease in sperm (progressive) motility and in plasma membrane and acrosomal integrity caused by H<sub>2</sub>O<sub>2</sub> (p < 0.05), whereas increased the T-AOC content and the activities of CAT and GPx antioxidant enzymes after 2 h of incubation at 37°C (p < 0.05). Further, the metabolomic analysis revealed that the addition of arbutin principally influenced lipid metabolism, and the Western blot analysis demonstrated that arbutin increased the sperm quality and the antioxidant capacity via the NRF2/GPX4 signalling. Together, arbutin preserves boar sperm during storage at 17°C by enhancing the antioxidant capacity via the NRF2/GPX4 signalling, laying the theoretical foundation for optimisation of the boar semen preservation diluent therefore facilitating the dissemination of superior porcine germplasm resources and improving the economic value.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 8","pages":"e70103"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimising Filtered Egg Yolk Extenders for Long-Term Cold Preservation of Ram Sperm: Effects of Osmolarity and Egg Yolk Concentration. 用于长期低温保存公羊精子的过滤蛋黄填充剂的优化:渗透压和蛋黄浓度的影响。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-08-01 DOI: 10.1111/rda.70106
Mohammad Hassan Nategh Ahmadi, Abdolah Mirzaei, Mohammad Reza Divar, Fatemeh Derafshani
{"title":"Optimising Filtered Egg Yolk Extenders for Long-Term Cold Preservation of Ram Sperm: Effects of Osmolarity and Egg Yolk Concentration.","authors":"Mohammad Hassan Nategh Ahmadi, Abdolah Mirzaei, Mohammad Reza Divar, Fatemeh Derafshani","doi":"10.1111/rda.70106","DOIUrl":"https://doi.org/10.1111/rda.70106","url":null,"abstract":"<p><p>Cold storage is a preferred method for short-term preservation of ram spermatozoa due to its superior fertility rates compared to cryopreservation. This study aimed to optimise the conditions for ram sperm preservation by evaluating the effects of osmolarity and egg yolk (EY) concentration in a syringe-filtered extender. In part A, semen samples were extended with solutions of varying osmolarities (330, 360, 390 and 420 mOsm/kg water). The 390 mOsm/kg solution demonstrated the best preservation of sperm viability, functional integrity and motility over 96 h of storage. In part B, different EY concentrations (10%, 15%, 20%, 25% and 30%) were assessed at the optimal osmolarity (390 mOsm/kg). A 20% EY concentration provided the most effective protection of sperm DNA, acrosome integrity and membrane functionality. Excessive EY levels (> 20%) negatively impacted sperm viability and induced higher lipid peroxidation. These findings emphasise the importance of balancing osmolarity and EY concentration for efficient cold storage of ram spermatozoa, enhancing the potential success of artificial insemination programs in sheep breeding.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 8","pages":"e70106"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved Pregnancy Outcomes in Beef Heifers Through Delayed Insemination of Sexed Semen. 通过延迟性授精改善肉用小母牛妊娠结局。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-08-01 DOI: 10.1111/rda.70111
R Kasimanickam, K Ratzburg, K Madsen, R Keckler
{"title":"Improved Pregnancy Outcomes in Beef Heifers Through Delayed Insemination of Sexed Semen.","authors":"R Kasimanickam, K Ratzburg, K Madsen, R Keckler","doi":"10.1111/rda.70111","DOIUrl":"10.1111/rda.70111","url":null,"abstract":"<p><p>This study evaluated the effect of the timing of Y-sorted sexed semen (SS) insemination after the onset of estrus on reproductive outcomes in beef heifers and examined the influence of AI sires and their sperm DNA fragmentation (%SDF) over time. Angus heifers (n = 718) from two locations were synchronised using a CIDR + Select-Synch protocol and blocked by age, body condition score, and reproductive tract score. Heifers expressing estrus were randomly assigned to AI at 12, 20, or 28 h post-estrus onset using SexedULTRA 4 M semen from one of three bulls. Post-thaw %SDF at 0, 12, and 24 h was assessed by acridine orange staining. Pregnancy per AI (P/AI) differed by timing: 12 h (45.4%), 20 h (48.1%), and 28 h (56.5%) (p < 0.05), with the 28 h group achieving significantly higher P/AI than the 12 h group. Stillbirth incidence and gender ratio (bull: heifer) did not differ significantly among groups. No overall difference in P/AI among sires was observed; however, a significant sire × time of AI interaction existed (p = 0.05), with Bull 2 showing the greatest improvement in P/AI at 28 h (up to 15.2 percentage points higher than earlier times). For Bulls 1 and 2, %SDF increased significantly from 0 to 24 h post-thaw, while Bull 3 showed no change. These findings indicate that delaying insemination to 28 h post-estrus enhances P/AI when using Y-sorted SS, potentially due to improved synchrony with ovulation and reduced exposure to sperm with increasing DNA damage.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 8","pages":"e70111"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12359292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Addition of Osteopontin for Freezing Epididymal Bovine Sperm Improves Post-Thaw Motility, Morphology and Membrane Functionality. 添加骨桥蛋白冷冻附睾牛精子改善解冻后运动、形态和膜功能。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-08-01 DOI: 10.1111/rda.70116
Carolina Montoya Ramírez, Alexandra Úsuga, Giovanni Restrepo Betancur, Ligia Johana Jaimes Cruz, Mariano Eliécer Acosta Lobo
{"title":"The Addition of Osteopontin for Freezing Epididymal Bovine Sperm Improves Post-Thaw Motility, Morphology and Membrane Functionality.","authors":"Carolina Montoya Ramírez, Alexandra Úsuga, Giovanni Restrepo Betancur, Ligia Johana Jaimes Cruz, Mariano Eliécer Acosta Lobo","doi":"10.1111/rda.70116","DOIUrl":"10.1111/rda.70116","url":null,"abstract":"<p><p>Cryopreservation of epididymal sperm is an important tool for preserving the germplasm of animals with high genetic value after death or due to clinical conditions. Nonetheless, its implementation remains challenging, as sperm are more susceptible to freezing and thawing. Osteopontin is a protein that has been linked to high fertilisation rates and antioxidant properties. The objective of this study was to evaluate the effect of adding osteopontin to the freezing extender on the post-thaw quality of bovine epididymal sperm. Sperm was collected from the epididymis cauda from 13 bovine testis-epididymal complexes from Bos indicus bulls. Each sample was supplemented with osteopontin at 0.1, 1.0 and 10 μg/mL, and a control treatment without osteopontin. The spermatozoa were extended and frozen in nitrogen vapour, then evaluated after thawing for motility, kinematics, morphology and plasma membrane integrity. Compared to the control treatment, the addition of osteopontin at 0.1, 1.0 and 10 μg/mL increased the total and progressive motility of frozen-thawed epididymal spermatozoa, with no differences among these three treatments. The use of osteopontin at 10 μg/mL increased the beat cross frequency (BCF) of spermatozoa; however, no other differences were found in the other kinematic parameters of thawed epididymal semen. An increase in the proportion of morphologically normal sperm was observed when the three concentrations of osteopontin were added to the freezing extender (0.1, 1.0 and 10 μg/mL). Similarly, all three concentrations of osteopontin improved the functional integrity of the plasma membrane of thawed epididymal sperm, regardless of the concentration used. It is concluded that adding 0.1 μM osteopontin to the freezing medium is enough to improve the quality of the frozen-thawed bovine epididymal spermatozoa, in an equivalent manner to the use of higher concentrations of this protein.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 8","pages":"e70116"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histological and Colour Doppler-Based Vascularity Analysis of Ovarian Follicles at Different Developmental Stages in Cattle. 牛不同发育阶段卵巢卵泡的组织学和彩色多普勒血管分析。
IF 1.7 3区 农林科学
Reproduction in Domestic Animals Pub Date : 2025-08-01 DOI: 10.1111/rda.70109
Mounir Mebarki, Mohamed Besbaci
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