Reproduction in Domestic Animals最新文献_第4页

Nongenomic Progesterone Receptors Mediated Bull Sperm Capacitation and Acrosome Reaction Are cAMP-PKA-Calcium Channel Dependent. 非基因组孕酮受体介导的公牛精子电容和顶体反应依赖于 cAMP-PKA-钙通道。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70045

Vikas Sachan, Atul Saxena, Anuj Kumar, Pratishtha Sharma, Jitendra Kumar Agrawal, Dilip Kumar Swain

{"title":"Nongenomic Progesterone Receptors Mediated Bull Sperm Capacitation and Acrosome Reaction Are cAMP-PKA-Calcium Channel Dependent.","authors":"Vikas Sachan, Atul Saxena, Anuj Kumar, Pratishtha Sharma, Jitendra Kumar Agrawal, Dilip Kumar Swain","doi":"10.1111/rda.70045","DOIUrl":"10.1111/rda.70045","url":null,"abstract":"Progesterone (P4) secreted from the cumulus cells mediates nongenomic signalling in sperm cells and induces capacitation and acrosome reaction in the oviduct. The induction of these events in bull spermatozoa is not fully understood. Recently, we reported the presence of membrane nongenomic P4 receptors in bull spermatozoa and the involvement of P4-nongenomic signalling through the involvement of complex signalling pathways with cannabinoid receptors in the regulation of bull sperm capacitation and acrosome reaction. Here in this study, using pharmacological agents, we report the involvement of cyclic adenosine 3'5' monophosphate-protein kinase A (cAMP-PKA) and calcium channels (sperm cation channel CatSper-and L-type) in the regulation of P4-dependent nongenomic induction of bull sperm capacitation and acrosome reaction. A total of 24 semen ejaculates were used in the study, and 1 picomolar (pM) and 1 micromolar (μM) P4 concentrations were used for capacitation and acrosome reaction induction, respectively in bull spermatozoa. Further, the P4-receptor blocker mifepristone (20 μM) and several other selective blockers KH7 (cAMP, 3 μM), P9115 (PKA, 10 μM), Nifedipine (L-type, 3 μM) and NNC 55-0396 (CatSper, 3 μM) were used for the exploration of downstream P4-signalling in the regulation of bull sperm capacitation and acrosome reaction. The sperm cells showed 50%-70% inhibition and reduction of P4-response in inducing capacitation and acrosome reaction after selective inhibition of these molecules during the study. Our results from the in vitro experiments in bull sperm cells suggested the involvement of cAMP-PKA, CatSper and L-type calcium channels during P4-induced bull sperm capacitation and acrosome reaction. These results also provided new insights into P4-nongenomic signalling in bull spermatozoa in the regulation of capacitation and acrosome reaction, further strengthening our understanding of these complex events in the oviduct.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70045"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thiamethoxam Exposure Impairs Oocyte Maturation via Induction of Lipid Metabolism and Mitochondrial Dysfunctions in Porcine. 噻虫嗪暴露通过诱导猪脂质代谢和线粒体功能障碍损害卵母细胞成熟。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70020

ChaoRui Wang, HuiLin Peng, XiuWen Yuan, HeWei Ji, YongNan Xu, Nam-Hyung Kim, YingHua Li

引用次数: 0

Application of Staining Methods to Compare Chromatin Condensation in Fresh and Freeze-Thawed Dog Semen. 应用染色法比较新鲜与冻融犬精液中染色质凝结。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70023

Elisa-Marie Laux, Abbas Farshad, Axel Wehrend, Mohamad Eid Hammadeh

{"title":"Application of Staining Methods to Compare Chromatin Condensation in Fresh and Freeze-Thawed Dog Semen.","authors":"Elisa-Marie Laux, Abbas Farshad, Axel Wehrend, Mohamad Eid Hammadeh","doi":"10.1111/rda.70023","DOIUrl":"10.1111/rda.70023","url":null,"abstract":"Various staining techniques have been used for canine sperm analysis, but direct comparisons using identical semen samples are lacking. This study aimed to assess the efficiency, time requirements and cost-effectiveness of different staining techniques: aniline blue, toluidine blue, acridine orange (AcO), chromomycin A3 (CMA3) and terminal deoxynucleotidyl transferase d-UTP nick-end labeling (TUNEL). Forty semen samples (20 fresh and 20 frozen-thawed) were used to assess chromatin condensation. Significant differences (p < 0.01) were found using two-factor repeated measures variance analysis. Aniline blue staining differed significantly (p < 0.01) from toluidine blue, AcO and CMA3 staining. A significant difference (p < 0.05) was observed between AcO and TUNEL staining for fresh sperm, with no significant differences between TUNEL and other methods. Correlations in fresh sperm samples showed r = 0.567 between AcO and aniline blue, r = 0.645 between AcO and CMA3, and correlations of 0.455 and 0.557 for aniline blue - toluidine blue and aniline blue - TUNEL, respectively. For frozen samples, significant differences were found between aniline blue and toluidine blue and AcO tests (p < 0.05), and between CMA3 and TUNEL staining (p < 0.01). Correlations in frozen samples showed r = 0.582 between AcO and aniline blue, r = 0.752 between AcO and CMA3, r = 0.698 between toluidine blue and aniline blue, and r = 0.536 between CMA3 and aniline blue. A minimal association was found between standard semen analysis and chromatin analysis. In conclusion, toluidine blue is effective for light microscopy staining, while CMA3 is recommended for fluorescence microscopy due to its simplicity, rapidity and cost-effectiveness.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70023"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Key Genetic Factors Influencing Reproductive Performance in Dairy and Meat Sheep Breeds of the Mediterranean Region. 确定影响地中海地区乳羊和肉羊品种繁殖性能的关键遗传因素。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70039

Maria Consuelo Mura, Giovanni Cosso, Mortadha Ouadday, Chadi Hosri, Joze Starič, Michella Nehme, Vincenzo Carcangiu, Sebastiano Luridiana

{"title":"Identifying Key Genetic Factors Influencing Reproductive Performance in Dairy and Meat Sheep Breeds of the Mediterranean Region.","authors":"Maria Consuelo Mura, Giovanni Cosso, Mortadha Ouadday, Chadi Hosri, Joze Starič, Michella Nehme, Vincenzo Carcangiu, Sebastiano Luridiana","doi":"10.1111/rda.70039","DOIUrl":"10.1111/rda.70039","url":null,"abstract":"This study aimed to detect genetic variations in key genes (BMPR1B, BMP15 and GDF9) that influence crucial reproductive traits, such as lambing rate and litter size in sheep. Understanding the genetic basis of these traits is essential for developing more efficient breeding strategies and selecting superior breeding stock, ultimately contributing to improved reproductive efficiency across different sheep breeds, production purposes and farming systems. We examined polymorphisms in the above genes across 500 adult ewes from three Mediterranean countries. The studied breeds included high-yielding dairy (Sarda), dual-purpose with a meat focus (Awassi) and medium-to-low dairy and meat breeds (Slovenian Bovška, Istrian Pramenka and Jezersko-Solčavska). Genetic variations were analysed and correlated with reproductive efficiency, including the interval in days from ram introduction to lambing based on lambing dates. This was used to assess the potential influence of these genes on follicular maturation and oocyte developmental competence. The analysis identified 15 polymorphisms distributed differently among the breeds, with the Sarda exhibiting 11 SNPs and the Awassi 13. The Slovenian breeds showed distinct genetic variation patterns potentially linked to their production goals. However, no significant association was observed between the identified polymorphisms and the analysed reproductive traits. Further studies on other genes regulating bone morphogenetic protein expression, such as the Gremlin 1 (GREM1) gene, may provide additional insights into the genetic mechanisms underlying reproductive traits.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70039"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Study of Testicular Hypoxia Adaptation in Tibetan Sheep. 藏羊睾丸缺氧适应的转录组学研究。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70037

Binyan Yu, Yanan Yang, Yijian Li, Rong Gao, Min Ma, Xinrong Wang

{"title":"Transcriptomic Study of Testicular Hypoxia Adaptation in Tibetan Sheep.","authors":"Binyan Yu, Yanan Yang, Yijian Li, Rong Gao, Min Ma, Xinrong Wang","doi":"10.1111/rda.70037","DOIUrl":"10.1111/rda.70037","url":null,"abstract":"The Tibetan sheep is a typical hypoxia-tolerant mammal, which lives on the plateau, at an altitude of between 2500 and 5000 m above sea level; the study of its hypoxic adaptation mechanism provides a reference for exploring the hypoxic adaptation mechanism of other animals. To grope for the genetic mechanism of adaptation to the hypoxic environment at the transcriptional level in Tibetan sheep testicular tissue, and to identify candidate genes and key pathways related to sheep adaptation, histological observation of testicular tissues from two sheep breeds was carried out using haematoxylin-eosin (HE) conventional staining. A total of 103 differentially expressed genes (DEGs) were authenticated in high altitude Tibetan sheep (ZYH) and low altitude Tibetan sheep (ZYM) by RNA sequencing technology (RNA-Seq), which included 50 up-regulated genes and 53 down-regulated genes. Functional analyses revealed several terms and pathways that were closely related to testis adaptation to the plateau. Several genes (including GGT5, AGTR2, EDN1, LPAR3, CYP2C19, IGFBP3, APOC3 and PKC1) were remarkably enriched in several pathways and terms, which may impact the Plateau adaptability of sheep by adjusting its reproductive activity and sexual maturation, and protecting Sertoli cells, various spermatocytes, and spermatogenesis processes. The results make a reasonable case for a better understanding of the molecular mechanisms of adaptation to altitude in sheep.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70037"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for Evaluating Testicular Function in Domestic Cats. 评价家猫睾丸功能的方法。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70042

Stepanka Homola, Sait Sendag, Davut Koca, Axel Wehrend

{"title":"Methods for Evaluating Testicular Function in Domestic Cats.","authors":"Stepanka Homola, Sait Sendag, Davut Koca, Axel Wehrend","doi":"10.1111/rda.70042","DOIUrl":"10.1111/rda.70042","url":null,"abstract":"This study aimed to describe the physiological sonomorphology of the cat testicle and to verify the findings by histological and endocrinological analyses. Furthermore, two methods of testicle measurement and volume calculation were compared. For the study, a clinical examination of the testicles was carried out in 39 cats. The testicles were measured with the Podany testimeter and using a sonographic examination. This was followed by a castration and histological examination of the testicle. The testosterone level was measured from a blood sample. The cat testicles showed a characteristic sonomorphology. The parenchyma was homogeneous, with a distinct echogenic mediastinum. The histological examination verified that there was spermatogenesis activity in testicular tissue. It was found that the testosterone concentrations varied greatly between the animals (0.1-4.40 ng/mL) although spermatogenesis was detected in all cats. Significant correlations were detected between right and left testicular volumes of the cats according to the measured values of sonography and Podany testimeter (p < 0.001). In addition, there was a significant and positive correlation between testosterone and testicular volume. Testosterone levels increase with increasing testicular volume according to the sonographic method (p < 0.05). With the help of the sonographic measurement, significantly higher testicular volumes are calculated than were compared with the measurement method with the Podany testimeter (p < 0.05). The present study is the first to provide detailed information and reliable data for the evaluation of the testicle size and volume in male cats as well as for sonomorphology, which can be used as comparison values for the andrological examination of this animal species.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70042"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonic Evaluation of the Corpus Luteum in Alpacas With Embryo Removal on Day 9 Post-Mating. 交配后第9天去胚羊驼黄体的超声评价。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70047

Ariadna Yáñez, Almudena Isabel Arrayás, Uri Harold Perez-Guerra, Alicia María López, Edita Yola Torres, Jesús Manuel Palomino

{"title":"Ultrasonic Evaluation of the Corpus Luteum in Alpacas With Embryo Removal on Day 9 Post-Mating.","authors":"Ariadna Yáñez, Almudena Isabel Arrayás, Uri Harold Perez-Guerra, Alicia María López, Edita Yola Torres, Jesús Manuel Palomino","doi":"10.1111/rda.70047","DOIUrl":"10.1111/rda.70047","url":null,"abstract":"The objective of the present study was to determine the diameter, area, circumference, and blood flow of the corpus luteum (CL) using transrectal ultrasonography in female alpacas with and without the presence of an embryo on Day 9 post-mating, the time of maternal recognition of pregnancy (MRP). For this purpose, 12 female alpacas with follicles ≥ 7 mm were mated with fertile males and treated with 0.0084 mg of buserelin acetate (Day 0). After that, the females were randomly classified into 2 groups (6 animals/group): (1) CL with embryo and (2) CL without embryo (embryo removed from the uterine horn on Day 9). In both groups, transrectal ultrasonography was performed on Day 2 to determine ovulation, and on Days 5, 7, 9, 11, and 13 to evaluate the CL. The data were analysed using repeated measures ANOVA. The diameter (mm), total area (mm2), circumference (mm), and blood flow (%) of the CL were significantly decreased in the CL without embryo group on Days 11 and 13 (6.9 ± 0.6 and 2.9 ± 1.1, 48.3 ± 5.6 and 12.3 ± 4.7, 22.4 ± 1.9 and 7.4 ± 2.5 and 39% and 55%, respectively) compared to the CL with embryo group (12.1 ± 0.9 and 12.1 ± 0.6, 111.1 ± 17.1 and 107.5 ± 8.8, 37.2 ± 2.8 and 37.0 ± 1.7, and 3% and 3%, respectively). In conclusion, the CL characteristics in alpacas with embryo removal changed drastically after Day 9, presenting progressive regression in size and blood flow from Day 11 to 13. These results might indicate that the presence of an embryo on Day 9 is necessary to trigger the signal that prevents luteolysis in this species.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70047"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Follicular B-Mode Echotexture Parameters at P4 Insert Removal and Their Association With Pregnancy Outcomes in Beef Cows Undergoing Timed AI Protocol. 采用定时人工智能技术的肉牛摘除P4插入物时卵泡b型超声图像参数及其与妊娠结局的关系

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70018

César Augusto Pinzón-Osorio, Dionet Keny Bellido-Quispe, Marco Alves Machado, Fernando Caetano de Oliveira, José Luiz Rodrigues, Marcelo Bertolini

{"title":"Follicular B-Mode Echotexture Parameters at P4 Insert Removal and Their Association With Pregnancy Outcomes in Beef Cows Undergoing Timed AI Protocol.","authors":"César Augusto Pinzón-Osorio, Dionet Keny Bellido-Quispe, Marco Alves Machado, Fernando Caetano de Oliveira, José Luiz Rodrigues, Marcelo Bertolini","doi":"10.1111/rda.70018","DOIUrl":"10.1111/rda.70018","url":null,"abstract":"This study aimed to determine the relationship between echogenicity and heterogeneity of ovarian structures and cyclicity status, estrus expression and pregnancy per artificial insemination (P/AI) in multiparous beef cows during timed-artificial insemination (TAI). An ultrasound ovarian examination was carried out in 406 crossbred suckled cows at progesterone (P4) insert removal on D8 of a TAI protocol and at pregnancy diagnosis 30 days after TAI. Follicular (antrum, wall and perifollicular stroma) and luteal morphometry, and echotexture and heterogeneity parameters were analysed on D8 and D30 after TAI, respectively. Follicles that did not reach divergence (< 8.5 mm) at P4 removal had higher antral echotexture and heterogeneity values (p < 0.0001) than those that surpassed divergence (> 8.5 mm). Lower follicular antrum echotexture levels at P4 removal positively correlated with subsequent estrus expression and pregnancy outcomes (p < 0.05), and negatively with follicle size (p < 0.0001). Luteal echotexture varied according to the originating follicle size (p < 0.05), with no differences between pregnant and non-pregnant cows 30 days after TAI. Cows had higher odds of estrus expression (Se, 70.61%; Sp, 61.05%; AUC 0.80; p < 0.0001) and attaining P/AI (Se, 64.52%; Sp, 61.05%; AUC 0.73; p < 0.0001) with lower follicular antrum echotexture (overall cut-off ≤ 14.25). The results show that echotexture values in antral follicles on D8 are strongly associated with pregnancy outcomes and may be useful for predicting success in TAI protocols.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70018"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progesterone and Oestradiol-17β Levels and Their Associations With Male Acceptance and Pregnancy in Female Alpacas (Vicugna pacos). 雌性羊驼的孕酮和雌二醇-17β水平及其与雄性接受和妊娠的关系

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70041

Toru Egi, Masaaki Hanada, Arisa Iiduka, Yuji Tokura, Rodrigo H Kawabata, Alejandro Bonifacio Flores, Tomás J Acosta

{"title":"Progesterone and Oestradiol-17β Levels and Their Associations With Male Acceptance and Pregnancy in Female Alpacas (Vicugna pacos).","authors":"Toru Egi, Masaaki Hanada, Arisa Iiduka, Yuji Tokura, Rodrigo H Kawabata, Alejandro Bonifacio Flores, Tomás J Acosta","doi":"10.1111/rda.70041","DOIUrl":"10.1111/rda.70041","url":null,"abstract":"Alpacas are important livestock animals in the Andean highlands of South America. However, their reproductive efficiency is low under natural conditions. In this study, we analysed the oestradiol-17β (E2) and progesterone (P4) levels in the serum and faecal samples of female alpacas before exposure to males for mating to determine whether E2 and P4 affect male acceptance and pregnancy in alpacas. In Experiment 1, faecal samples were collected from nine female alpacas prior to exposure to males for mating to evaluate the effects of premating faecal hormone levels on mating behaviour. In Experiment 2, four of nine female alpacas, which had not previously accepted mating, were injected intramuscularly with oestradiol benzoate (EB). Mating receptivity after EB administration, serum E2 levels before and after EB administration and pregnancy were then evaluated. In both experiments, P4 and E2 were measured in serum and faecal samples using ELISA kits after extraction with diethyl ether and methanol respectively. In Experiment 1, of 23 contacts with males in the mating test, females accepted males 12 times and rejected them 11 times. The proportion of females rejecting males increased as the premating faecal P4 level increased (p = 0.021) and the proportion of females accepting males increased as the faecal E2 level increased (p = 0.035). In Experiment 2, serum E2 levels were higher 2 h after injection than before EB injection (p < 0.05). Alpacas that refused to mate before injection accepted mating 2 h after EB treatment. All alpacas with faecal E2 levels more than 0.5 ng/mg of dry matter before mating had higher P4 levels a month after mating than those with low E2 levels (p < 0.05) and were pregnant. In conclusion, this study showed that measurement of faecal E2 levels can help in determining the appropriate breeding time and that EB treatment improves reproductive performance in alpacas.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70041"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR-Based Amplification of Amelogenin Gene for Ovine Sex Determination Using Primers of Different GC Percentage. 不同GC百分比引物pcr扩增羊绒原蛋白基因用于绵羊性别测定。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI: 10.1111/rda.70026

Anupama Karimpat, Ashish Mishra, Sudhir Chandra Roy, Ippala Janardhan Reddy

{"title":"PCR-Based Amplification of Amelogenin Gene for Ovine Sex Determination Using Primers of Different GC Percentage.","authors":"Anupama Karimpat, Ashish Mishra, Sudhir Chandra Roy, Ippala Janardhan Reddy","doi":"10.1111/rda.70026","DOIUrl":"10.1111/rda.70026","url":null,"abstract":"The identification of species and their sex from small biological samples is of scientific interest in forensic science. Various identification techniques have been developed; however, DNA-based PCR is the most specific and sensitive technique compared to protein-based methods. Although PCR amplification of the amelogenin (AMEL) has been used in different species for sex determination, the reliability of the AMEL test may sometimes be challenged due to amplification failure of AMEL Y in males, resulting in incorrect gender identification. Therefore, this study aimed to establish a simple, reliable and accurate PCR protocol for the amplification of the AMEL gene from blood gDNA isolated by a single-step DNA isolation method using primers of different GC% to ascertain the sex of ovine. This methodology may also be applicable to various biological samples for sex determination. It was concluded that the touchdown PCR was more suitable for GC-rich primers and low GC% primers were suitable with modified conventional PCR for gender identification. The use of PCR enhancers at denaturation temperatures of 94°C and 95°C was found ineffective for the amplification of AMEL to determine the sex. In summary, all primers used showed successful amplification.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 3","pages":"e70026"},"PeriodicalIF":1.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0