可育和欠育母狗子宫内膜的组织学研究。

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Gail R McRae, Marco A Coutinho da Silva, Erin E Runcan, Julie A Stephens, Christopher Premanandan
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引用次数: 0

摘要

子宫内膜活检历来作为一种诊断工具,在一些家养物种中作为育种管理的一部分。在母马中,子宫内膜活检的结果可能与母马怀孕至足月的能力有关。子宫内膜活检在犬的价值尚未完全确定。在本研究中,在晚期停经/早期停经的低育母犬的全层子宫活检中发现了炎症、纤维化和子宫内膜囊肿的特征,并与可育母犬的相同发现进行了比较。假设是低生育能力的母狗会有更多的炎症、纤维化和子宫内膜囊肿。对照病例(n = 103)在剖宫产时在剖宫产部位进行全层子宫活检,之前提交的来自俄亥俄州生殖服务中心的样本(n = 263)为低生育能力样本。对这些病例进行盲法、随机分组和评估。使用卡方检验或费雪精确检验来比较对照和实验样本之间的分类变量。采用Wilcoxon秩和检验比较各组之间的年龄。总的来说,与对照组相比,在不孕不育组中观察到的炎症更频繁。与可育组相比,淋巴浆细胞性炎症在低育组中更为常见。两组间腺周纤维化和子宫内膜囊肿发生率无显著差异。这些结果表明,犬子宫活检的显微评估可能更适合于检测子宫内膜的炎症变化和孤立的子宫内膜囊肿,以及孤立的纤维化病变,与慢性炎症相比,这些病变对生育的影响可能较小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histological Studies in the Endometrium of Fertile and Subfertile Bitches.

Endometrial biopsies have been used historically as a diagnostic tool as part of breeding management in some domestic species. In the mare, the findings in an endometrial biopsy can relate to the ability of that mare to carry a pregnancy to term. The value of the endometrial biopsy in the canine has not been fully established. In this study, the presence of inflammation, fibrosis and endometrial cysts was characterised in full-thickness uterine biopsies from subfertile bitches in late diestrus/early anestrus and compared to the same findings in fertile bitches. The hypothesis was that subfertile bitches would have more inflammation, fibrosis and endometrial cysts. Full-thickness uterine biopsies were taken at the hysterotomy site at the time of C-section for control cases (n = 103), and previous submissions were collected from The Ohio State Reproductive Service as subfertile samples (n = 263). The cases were blinded, randomised and evaluated. Chi-squared or Fisher's exact tests were used to compare categorical variables between control and experimental samples. The Wilcoxon rank-sum test was used to compare ages between the groups. Overall, inflammation was observed more frequently in the subfertile group in comparison to the control group. Lymphoplasmacytic inflammation was observed more frequently in the subfertile group when compared to the fertile group. The incidence of periglandular fibrosis and endometrial cysts was not significantly different between the two groups. These results indicate that microscopic evaluation of canine uterine biopsies may be more suited to detecting inflammatory changes in the endometrium and isolated endometrial cysts, as well as isolated fibrotic lesions, which may be less impactful on fertility compared to chronic inflammation.

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来源期刊
Reproduction in Domestic Animals
Reproduction in Domestic Animals 农林科学-奶制品与动物科学
CiteScore
3.00
自引率
5.90%
发文量
238
审稿时长
4-8 weeks
期刊介绍: The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.
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