Reproduction in Domestic Animals最新文献_第5页

TLR-2 and TLR-4 mRNA expression in different grades of histopathological lesions of equine endometrium from follicular phase. 不同等级卵泡期马子宫内膜组织病理学病变中 TLR-2 和 TLR-4 mRNA 的表达。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14657

Marta Cerveira-Pinto, Anna Wójtowicz, Maria Anjos Pires, Pawel Kordowitzki, Dariusz Skarzynski, Graça Ferreira-Dias, Anna Szóstek-Mioduchowska, Ana Amaral

{"title":"TLR-2 and TLR-4 mRNA expression in different grades of histopathological lesions of equine endometrium from follicular phase.","authors":"Marta Cerveira-Pinto, Anna Wójtowicz, Maria Anjos Pires, Pawel Kordowitzki, Dariusz Skarzynski, Graça Ferreira-Dias, Anna Szóstek-Mioduchowska, Ana Amaral","doi":"10.1111/rda.14657","DOIUrl":"10.1111/rda.14657","url":null,"abstract":"Increased synthesis and deposition of collagen (COL) in the extracellular matrix (ECM) of equine endometrium contributes to endometrosis. Toll-like receptors (TLRs) are transmembrane receptors involved in the innate immune response, recognized for their role in antigen recognition and previously associated with equine endometritis. The TLRs not only recognize pathogen-associated molecular patterns but also regulate inflammations, fibrosis and cancer. The aim of this study was to explore the relationship between TLR expression at different stages of Kenney and Doig's (K-D) grading and COL1 expression during the follicular phase of the oestrous cycle. Forty samples of endometrial tissues were collected post-mortem from mares on the follicular phase of the oestrous cycle (10 samples of each K-D category). Relative mRNA transcription of TLR-2, TLR-4 and COL1A2 genes was assessed using qPCR, and COL1 protein expression by Western blot analysis. The COL1A2 transcription increased in category IIB when compared to categories I, IIA and III endometria (p < .01). The relative protein abundance of COL1 showed no significant differences between endometrial categories (p > .05). As for the TLRs mRNA transcription, TLR-2/-4 transcripts increased in IIA when compared to the other K-D endometria categories (p < .05). Our findings suggest that TLRs may be involved in the initiation of the endometrial inflammatory response. Additional studies are needed to explore TLRs' potential role as diagnostic markers for monitoring inflammation progression and fibrosis development, as well as their involvement in the mechanisms underlying fibrotic pathways.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 3 ","pages":"e14657"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclodextrin-Cholesterol Alone or in Combination With Cyclodextrin-Vitamin E Improves Bull Sperm Cryopreservation in a Soybean Lecithin Extender. 环糊精-胆固醇单独使用或与环糊精-维生素 E 结合使用可改善公牛精子在大豆卵磷脂扩展剂中的冷冻保存。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14728

Djemouai Boukhalfa, Karim Benhenia, Asmaa Sayah, Ahcene Kourat, Boubakeur Safsaf

{"title":"Cyclodextrin-Cholesterol Alone or in Combination With Cyclodextrin-Vitamin E Improves Bull Sperm Cryopreservation in a Soybean Lecithin Extender.","authors":"Djemouai Boukhalfa, Karim Benhenia, Asmaa Sayah, Ahcene Kourat, Boubakeur Safsaf","doi":"10.1111/rda.14728","DOIUrl":"https://doi.org/10.1111/rda.14728","url":null,"abstract":"Combining cholesterol-loaded methyl-β-cyclodextrin (CD-CHL) with vitamin E-loaded methyl-β-cyclodextrin (CD-Vit E) to combat cold shock and oxidative stress during sperm cryopreservation in soybean lecithin extenders remains unexplored. Thus, the current study aimed to investigate the effect of treating bull sperm with CD-CHL and CD-Vit E prior to cryopreservation in a soybean lecithin extender. Sperm collected from eight fertile bulls were pooled and split into six aliquots. Five aliquots were treated, in a Tris-based extender, with CD-CHL (2 mg/120 × 106 cells/mL) and either 0, 0.5, 1.0, 1.5 or 2 mg CD-Vit E/120 × 106 cells/mL. The control aliquot was diluted in a Tris-based extender without further supplementation. After incubation at 22°C for 15 min and addition of a soybean lecithin extender, all aliquots were equilibrated for 2 h at 4°C and then cryopreserved in liquid nitrogen. Computer-assisted sperm analysis (CASA) was used to explore the different sperm motility parameters, hypo-osmotic swelling test to determine membrane functionality and fluorescein isothiocyanate-conjugated Aeachis hypogaea (peanut) agglutinin (FITC-PNA) to quantify acrosome integrity. The effect of oxidative stress on the sperm membrane was assessed through lipid peroxidation measurement. Compared to control, CD-CHL alone improved significantly (p < 0.05) all CASA motility parameters, membrane functionality and acrosome integrity of thawed sperm. The membrane functionality was more significantly (p < 0.05) improved when 0.5 mg CD-Vit E was combined with CD-CHL. Concerning lipid peroxidation, no significant differences (p > 0.05) in malondialdehyde (MDA) levels were registered between groups. In conclusion, the combination of CD-CHL and CD-Vit E demonstrated a significant positive effect on the cryopreservation of bull sperm in a soybean lecithin extender.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14728"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new hormonal protocol supports early development of in vitro-produced embryos after transfer to anoestrus mares. 一种新的激素方案支持体外生产的胚胎移植到发情母马体内后的早期发育。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14622

Carmen C Muñoz-García, Marcos Luis-Calero, Pablo Fernández-Hernández, Alba Pizarro-Hernández, Raúl Fernández-González, Alfonso Gutiérrez-Adán, María Jesús Sánchez-Calabuig, Juan Cuervo-Arango, Lauro González-Fernández, Beatriz Macías-García

引用次数: 0

Effect of long-term oral administration of the flavonoid quercetin on the antioxidant profile of young and adult reproductive rabbit does. 长期口服黄酮类化合物槲皮素对幼年和成年生殖兔抗氧化谱的影响。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14639

A Vicente-Carrillo, D Jordán-Rodríguez, E Cáceres-Martín, A Gómez-León, P G Rebollar, P L Lorenzo, R M García-García, M Arias-Álvarez

{"title":"Effect of long-term oral administration of the flavonoid quercetin on the antioxidant profile of young and adult reproductive rabbit does.","authors":"A Vicente-Carrillo, D Jordán-Rodríguez, E Cáceres-Martín, A Gómez-León, P G Rebollar, P L Lorenzo, R M García-García, M Arias-Álvarez","doi":"10.1111/rda.14639","DOIUrl":"https://doi.org/10.1111/rda.14639","url":null,"abstract":"In industrialized farms, rabbit does undergo intensive production rhythms which overlap lactation and gestation, leading to a high energy mobilization and increasing oxidative stress. Accordingly, we hypothesize that administration of the flavonoid quercetin (QUR) may improve the antioxidant status of young and adult rabbit reproductive females. In this study, the effect of daily oral administration of 300 mg/kg QUR for 8 weeks was assessed on the antioxidant profile of 24 New Zealand × Californian rabbit does, assigned to 4 experimental groups: rearing young (8-16 weeks old) and adult does at the end of their reproductive life (12-14 months old, with at least 3-4 reproductive cycles) treated (YQ and AQ) or not (YC and AC) with QUR, respectively. Plasma glutathione (GSH), as well as serum superoxide dismutase (SOD) and malondialdehyde (MDA) were measured during the experimental period. To assess the health status of the animals, a physical examination was also performed. GSH plasma concentrations were significantly higher in young does at weeks 1 and 4, but not at week 8 of the experiment, irrespectively of QUR administration. An increase in GSH plasma concentration was observed during the 8-week experiment in both AQ and AC groups. Furthermore, QUR administration did not alter either SOD or MDA serum activity and concentration in any group during the experimental period. Physical examination revealed no differences between the experimental groups. In conclusion, under our experimental conditions, QUR did not modify the general clinical or the antioxidant profile of young and adult reproductive rabbit females.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 3 ","pages":"e14639"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonographic Assessment of Testicular Biometry and Arterial Blood Flow in Pre- and Postpubertal Miranda Donkeys: Correlations With Semen Quality Parameters. 米兰达驴青春期前后睾丸生物测量和动脉血流的超声波评估：与精液质量参数的相关性

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14727

A Martins-Bessa, M Quaresma, M Novoa, P M Bartlewski, S Silva

{"title":"Ultrasonographic Assessment of Testicular Biometry and Arterial Blood Flow in Pre- and Postpubertal Miranda Donkeys: Correlations With Semen Quality Parameters.","authors":"A Martins-Bessa, M Quaresma, M Novoa, P M Bartlewski, S Silva","doi":"10.1111/rda.14727","DOIUrl":"https://doi.org/10.1111/rda.14727","url":null,"abstract":"The main goals of this study were to document and compare the normal ranges of testicular haemodynamic parameters in pre- (aged 8-12 months; n = 4) and postpubertal (aged 24-259 months; n = 16) Miranda donkeys in the breeding season, and to correlate animal biometric data and testicular Doppler indices with basic semen quality parameters of sexually mature jacks. Colour and pulsed-Doppler ultrasonography were employed to assess blood flow in the left and right distal supratesticular artery (DsTA) and their marginal branches (marginal arteries-MA). Peak systolic velocity (PSV), end diastolic velocity (EDV), pulsatility index (PI) and resistive index (RI) were evaluated in both blood vessels, and TAMV (time-averaged mean velocity), TABF (total arterial blood flow) and TABF rate (TABF-R) were calculated for MA. The mean diameter of MA was greater (p < 0.05; 0.24 ± 0.05 vs. 0.19 ± 0.05 cm; mean ± SD) but TABF-R was less (p < 0.05; 0.004 ± 0.004 vs. 0.02 ± 0.01 mL/s/cm3) in sexually mature donkeys compared with prepubertal animals. Apart from RI values for the right testicle of prepubertal donkeys, PI and RI were consistently greater (p < 0.05) in DsTA compared with MA. Significant correlations were found among select biometric and haemodynamic attributes of the testes (height, width and length, TV, TTV and PSV-ST) and ejaculate characteristics (volume, sperm defects-total, head and midpiece) in sexually mature donkeys (n = 8). The present results highlight the importance of scrotal ultrasonography for the reproductive assessment of jacks and provide reference values, based on the available subpopulation of Miranda donkeys that can be used in their clinical and reproductive management and research, or conservation programmes.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14727"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Placentome Morphology in the Last Two Periods of Pregnancy in Hair Goats (Capra aegagrus hircus). 评估毛山羊（Capra aegagrus hircus）妊娠最后两个时期的胎盘形态。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14731

Fatma İşbilir, Banu Kandil, İhsan İşbilir, Davut Koca, Barış Can Güzel

{"title":"Evaluation of Placentome Morphology in the Last Two Periods of Pregnancy in Hair Goats (Capra aegagrus hircus).","authors":"Fatma İşbilir, Banu Kandil, İhsan İşbilir, Davut Koca, Barış Can Güzel","doi":"10.1111/rda.14731","DOIUrl":"https://doi.org/10.1111/rda.14731","url":null,"abstract":"This study was carried out on pregnant uteruses obtained from healthy Hair goats (Capra aegagrus hircus). A total of thirteen pregnant uteruses, six second and seven third trimesters, were used. Morphometrically, placentome numbers, lengths, widths and depths were measured. Randomly selected placentomes from the second and third trimesters were stained with the triple staining method. Caruncular and cotyledonary areas, capillary numbers and areas were measured using Qupath v0.5.0 software. The Minitab (version 21.4.1) was used for statistical analysis. While no statistical difference was observed in the number of placentomes between trimesters (p > 0.05), placentome length, width and depth were higher in the third trimester compared to the second trimester (p < 0.001). No difference was observed in the number of caruncular and cotyledonary capillaries in the second trimester. In the second trimester, the caruncular capillary area was higher than the cotyledonary capillary area (p < 0.05). Both caruncular and cotyledonary capillary area parameters were higher in the third trimester than in the second trimester (p < 0.001). In the third trimester, the caruncular capillary area was higher than the cotyledonary capillary area (p < 0.001). The number of caruncular capillaries and cotyledonary capillaries was statistically significantly higher in the third trimester compared to the second trimester. In addition, the number of cotyledonary capillaries was higher than that of caruncular capillaries in the third trimester (p < 0.001). A positive and significant correlation was found between the day of pregnancy and the number of placentomes in the second trimester (p < 0.05). No correlation was observed between the day of pregnancy and the number of placentomes in the third trimester. Vascular area density showed a faster development in foetal tissue than in maternal tissue. Placentome size and angiogenesis increased with the progression of pregnancy.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14731"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced assisted reproduction technologies in endangered mammalian species. 濒危哺乳动物物种的先进辅助生殖技术。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14700

Thomas Bernd Hildebrandt, Susanne Holtze

{"title":"Advanced assisted reproduction technologies in endangered mammalian species.","authors":"Thomas Bernd Hildebrandt, Susanne Holtze","doi":"10.1111/rda.14700","DOIUrl":"https://doi.org/10.1111/rda.14700","url":null,"abstract":"A new synergistic approach of classical conservation strategies combined with advanced assisted reproduction technologies (aART) allows for protection and rescue of endangered keystone species at the brink of extinction, which can help to safeguard complex ecosystems. Reproduction biology and management in mammal species is not only challenging in regards to their diverging sizes, anatomy, and often unknown physiology; it also requires customized training or chemical restraint protocols for safe handling. Besides these general challenges, there are several new assisted reproduction techniques (ART) specifically tailored to critically endangered mammals. The current portfolio of ART in these mammalian taxa is ranging from sexual cycle characterization and manipulation, semen collection and cryopreservation, artificial insemination, biobanking of living cells, oocyte collection, in vitro fertilization (IVF), and embryo production, embryo transfer as well as stem cell-derived in vitro gametogenesis for generating gametes in culture. The article covers advanced assisted reproduction technologies (aART), success and challenges, as well as ethical implications.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 3 ","pages":"e14700"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro-produced blastocysts. Cryotop 玻璃化系统可同时冷冻保存大量猪体外生产的囊胚。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14600

A González-Plaza, M Garcia-Canovas, I Parrilla, H Rodriguez-Martinez, M A Gil, E A Martinez, C Cuello

引用次数: 0

Development of an equine endometrial histology scoring system to complement the Kenney-Doig scale. 开发马子宫内膜组织学评分系统，以补充 Kenney-Doig 量表。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14614

I Martínez-Bartolomé, J Masot, C Serres

引用次数: 0

Bovine serum albumin inclusion in the thawing extender improves boar sperm membrane and acrosomal integrity. 在解冻扩展剂中加入牛血清白蛋白可改善公猪精子膜和顶体的完整性。