Reproduction in Domestic Animals最新文献_第5页

Livestock Blastoids: In Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies. 家畜囊胚：胚胎发生和生殖技术的体外囊胚模型。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70262

Cristina A Martinez-Serrano, Jun Wu

{"title":"Livestock Blastoids: In Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies.","authors":"Cristina A Martinez-Serrano, Jun Wu","doi":"10.1111/rda.70262","DOIUrl":"https://doi.org/10.1111/rda.70262","url":null,"abstract":"Understanding early embryogenesis in livestock remains a persistent bottleneck in both reproductive biology and animal breeding. Work in domestic species has long been limited by restricted access to embryos, ethical considerations, and the complexity of peri-implantation development, making mechanistic studies difficult. Recent advances in stem cell biology are beginning to shift this. Blastocyst-like structures, or blastoids, now offer a practical way to model early development in vitro. Blastoids capture key features of natural blastocysts, including overall structure, lineage composition, and gene expression patterns, while being far more accessible experimentally. In livestock species, especially cattle and pigs, blastoid models are starting to gain traction. Livestock blastoids provide a tractable system to study lineage specification, epiblast-extraembryonic interactions, and early conceptus development. They also open the door to improving assisted reproductive technologies by enabling controlled, scalable experimentation that is not feasible with embryos alone. In this review, we summarize recent advances in the generation of livestock blastoids and discuss how these systems can be used to study early development across livestock species and to inform next-generation reproductive technologies. We highlight current limitations and outline key challenges ahead, including how blastoid models can be combined with genome editing, stem cell engineering and related embryo-based approaches to improve reproductive efficiency in domestic animals.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70262"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Sucrose and Trehalose Supplementation on Zebrafish (Danio rerio) Sperm Quality During Cold Storage. 添加蔗糖和海藻糖对斑马鱼冷藏精子质量的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70256

Blenda R N Vilela, Pilar Vallejo-Soto, Carlos Montero-Palma, Manuel Hidalgo, Nahúm Ayala, Rosario Moyano, Luis D S Murgas, Isabel Ortiz, Jesús Dorado

引用次数: 0

Restoration of Motility in Acrosome-Reacted Boar Spermatozoa Induced by the Ionophore 4-Br-A23187. 离子载体4-Br-A23187诱导顶体反应的猪精子运动能力的恢复。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-10-01 DOI: 10.1111/rda.70236

David Martín-Hidalgo, Violeta Calle-Guisado, Beatriz Macías-García, Lauro González-Fernández

{"title":"Restoration of Motility in Acrosome-Reacted Boar Spermatozoa Induced by the Ionophore 4-Br-A23187.","authors":"David Martín-Hidalgo, Violeta Calle-Guisado, Beatriz Macías-García, Lauro González-Fernández","doi":"10.1111/rda.70236","DOIUrl":"https://doi.org/10.1111/rda.70236","url":null,"abstract":"The acrosome reaction (AR) is a calcium-dependent exocytotic event required for successful fertilization. Calcium ionophores are commonly used to induce AR in vitro; however, this treatment is associated with a significant loss of sperm motility. The aim of this study was to evaluate the recovery of motility in boar spermatozoa after ionophore-induced AR and to assess the role of extracellular calcium. Boar spermatozoa were incubated with 10 μM 4-Br-A23187 to induce AR. Then, spermatozoa were washed and incubated for 30 min in a recovery medium containing either 1 mM calcium or 5 mM EGTA. The AR was analysed by flow cytometry, and motility parameters were assessed using a CASA system. Ionophore treatment induced AR and caused a marked reduction in sperm motility, which was partially recovered in the presence of 5 mM EGTA, but not in the presence of 1 mM calcium. These results suggest that extracellular calcium inhibits the restoration of motility after ionophore-induced AR in boar spermatozoa.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 3 ","pages":"e70236"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13627754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uterine Dysfunctions in Non-Pregnant and Pregnant Small Animals. 未怀孕和怀孕小动物的子宫功能障碍。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70281

Sandra Goericke-Pesch

引用次数: 0

Special Issue: Proceedings of the Joint 29th Annual European Society for Domestic Animal Reproduction (ESDAR) Conference & 4th European College of Animal Reproduction (ECAR) Symposium, 17-19 September 2026, Olsztyn, Poland. 特刊：第29届欧洲家养动物繁殖学会（ESDAR）联合年度会议暨第四届欧洲动物繁殖学院（ECAR）研讨会论文集，2026年9月17日至19日，波兰奥尔什廷。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70276

引用次数: 0

Pregnancy Outcomes Following Artificial Insemination and Embryo Transfer in Dairy Cows-Expectations vs. Reality. 奶牛人工授精和胚胎移植后的妊娠结局：期望与现实。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70283

Ana Clara Canto Souza, Pat Lonergan

{"title":"Pregnancy Outcomes Following Artificial Insemination and Embryo Transfer in Dairy Cows-Expectations vs. Reality.","authors":"Ana Clara Canto Souza, Pat Lonergan","doi":"10.1111/rda.70283","DOIUrl":"10.1111/rda.70283","url":null,"abstract":"Bovine embryo transfer (ET) has been a consistently growing market worldwide with in vitro produced (IVP) embryos now far outnumbering those produced by traditional superovulation. Because transfer of a viable blastocyst-stage embryo to the uterus bypasses many of the potential biological causes of early pregnancy failure associated with artificial insemination (AI; anovulation, sperm transport, fertilization failure, early embryonic death), pregnancy success should logically be higher following ET than following AI. However, this is not always the case. One of the main factors contributing to this paradox is pregnancy loss following ET, particularly with IVP embryos. This greater embryo mortality presents a significant obstacle to more widespread use of IVP embryos. Improvements in embryo culture media as well as in our understanding of the underlying physiological and molecular regulation of early embryo development and embryo-maternal communication leading to a successful pregnancy will significantly contribute to improved reproductive efficiency and sustainability in agri-food production.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 2 ","pages":"e70283"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13538270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin Enhances Embryo Quality in Japanese Black Cows During the Hot Season. 褪黑素对炎热季节日本黑牛胚胎质量的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70317

Tomohiro Isobe, Yukina Yoshimura, Yoshihisa Ikebata

{"title":"Melatonin Enhances Embryo Quality in Japanese Black Cows During the Hot Season.","authors":"Tomohiro Isobe, Yukina Yoshimura, Yoshihisa Ikebata","doi":"10.1111/rda.70317","DOIUrl":"10.1111/rda.70317","url":null,"abstract":"Melatonin is a potent antioxidant hormone that influences bovine reproductive function; however, its practical effects on embryo production under heat stress remain unclear. This study evaluated whether short-term melatonin administration improves embryo production, reduces oxidative stress, and affects pregnancy outcomes in Japanese Black cows using ovum pick-up and in vitro embryo production (OPU-IVP) and multiple ovulation and embryo transfer (MO-ET) systems. In Experiment 1, cows received subcutaneous melatonin (120 mg) or saline on Days 2 and 8 before OPU during the hot season in a crossover design. Melatonin treatment significantly increased the number of freezable embryos obtained by OPU-IVP (rate ratio = 2.85, p < 0.01) and tended to reduce 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations in the mixture of follicular fluid and blood surrounding the ovaries. In Experiment 2, the effects of melatonin treatment before embryo recovery (ER) following superovulation were evaluated during the hot and cooler seasons. A single injection had no effect, whereas two injections given 7 and 15 days before ER significantly increased the number of freezable embryos during the hot season (rate ratio = 1.48, p < 0.05). Melatonin treatment significantly decreased serum 8-OHdG concentrations, indicating attenuation of systemic oxidative stress. Moreover, frozen-thawed in vivo embryos derived from melatonin-treated donors showed a tendency towards improved pregnancy outcomes in repeat-breeder (RB) cows. These findings suggest that targeted melatonin administration is a practical strategy for improving embryo quality under heat stress conditions and may be associated with improved pregnancy outcomes following ET in RB cows.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 9","pages":"e70317"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13551379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Puerperium in the Modern Dairy Cow: A Review. 现代奶牛产褥期研究综述。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70303

Michael J Morris, Kristen C Jaggernath, Adam D Martin, Reshmi S Ramdass

{"title":"The Puerperium in the Modern Dairy Cow: A Review.","authors":"Michael J Morris, Kristen C Jaggernath, Adam D Martin, Reshmi S Ramdass","doi":"10.1111/rda.70303","DOIUrl":"10.1111/rda.70303","url":null,"abstract":"The puerperium represents a critical physiological period during which the bovine reproductive tract transitions from pregnancy to renewed fertility. In the modern high-producing dairy cow, this transition is challenged by profound metabolic, endocrine, immunological, and structural demands that collectively influence uterine health, ovarian function, and subsequent reproductive performance. This review examines current understanding of the physiology of the puerperium in dairy cattle, with particular emphasis on uterine involution, immune clearance of postpartum contamination, endocrine regulation, and resumption of ovarian cyclicity. Further, it contrasts high-yielding Holsteins with fertility selected dairy populations. Normal uterine involution involves coordinated myometrial contraction, tissue remodelling, endometrial regeneration, and tightly regulated inflammatory responses. Failure of these processes predisposes cows to postpartum uterine disorders, including retained fetal membranes, metritis, endometritis (purulent vaginal discharge with cytological confirmation), and pyometra, which remain major contributors to subfertility and economic loss. Central to the pathophysiology of puerperal disease is negative energy balance, which disrupts immune competence, alters hepatic steroid metabolism, impairs ovarian signalling, and compromises oocyte and embryo quality. Emerging evidence highlights the complex interplay between metabolism, immunity, and the uterine microbiome, shifting current perspectives away from pathogen-centric models toward host resilience. Advances in biomarkers, genomic selection, and precision monitoring offer new opportunities for targeted reproductive management. Ultimately, optimisation of transition period management remains the cornerstone of supporting physiological puerperal recovery and sustaining reproductive efficiency in modern dairy systems.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 2 ","pages":"e70303"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13538273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Issue: Proceedings of the Joint 29th Annual European Society for Domestic Animal Reproduction (ESDAR) Conference & 4th European College of Animal Reproduction (ECAR) Symposium, 17-19 September 2026, Olsztyn, Poland. 特刊：第29届欧洲家养动物繁殖学会（ESDAR）联合年度会议暨第四届欧洲动物繁殖学院（ECAR）研讨会论文集，2026年9月17日至19日，波兰奥尔什廷。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70274

引用次数: 0

Effects of Post-Thaw Toll-Like Receptor 1/2 Agonist and Antagonist Exposure on Motility Subpopulations and Functional Characteristics of Bovine Spermatozoa. 解冻后toll样受体1/2激动剂和拮抗剂暴露对牛精子运动亚群和功能特性的影响。

IF 1.7 3区农林科学

Reproduction in Domestic Animals Pub Date : 2026-09-01 DOI: 10.1111/rda.70321

Chihiro Kanno, Toju Oshima, Shogo Sato, Yosuke Maeda, Fumiaki Takahashi

{"title":"Effects of Post-Thaw Toll-Like Receptor 1/2 Agonist and Antagonist Exposure on Motility Subpopulations and Functional Characteristics of Bovine Spermatozoa.","authors":"Chihiro Kanno, Toju Oshima, Shogo Sato, Yosuke Maeda, Fumiaki Takahashi","doi":"10.1111/rda.70321","DOIUrl":"https://doi.org/10.1111/rda.70321","url":null,"abstract":"Toll-like receptor 2 (TLR2) has been implicated in fertilization-related functions of bovine spermatozoa, but post-thaw responses to pharmacological TLR1/2 modulation across kinematic and membrane-related characteristics remain less defined. One ejaculate from each of four Holstein bulls was used and semen was exposed after thawing to a TLR1/2 agonist, a TLR1/2 antagonist or no additive. Five independent experimental repetitions per bull were performed using separate straws from the same ejaculate; therefore, the biological donor sample size was four bulls. Computer-assisted sperm analysis and k-means clustering identified six kinematically defined motility subpopulations and flow cytometry assessed plasma membrane and acrosomal integrity, membrane fluidity and mitochondrial superoxide-related fluorescence using MitoSOX Red. At 3 h, antagonist exposure reduced total motility compared with control and agonist treatments (30.1% ± 6.3% vs. 40.4% ± 5.6% and 37.7% ± 5.8%, respectively; adjusted p = 0.001). Conversely, antagonist-treated samples contained more spermatozoa with intact plasma membrane and acrosome than agonist-treated samples (52.4% ± 3.4% vs. 48.4% ± 3.1%; adjusted p = 0.001) and more viable spermatozoa with low MitoSOX fluorescence (31.1% ± 6.1% vs. 22.4% ± 4.7%; adjusted p = 0.001). Agonist exposure increased the proportion of viable spermatozoa with high membrane fluidity and was associated with higher MitoSOX fluorescence than antagonist exposure. These findings describe distinct post-thaw functional profiles following pharmacological TLR1/2 agonist and antagonist exposure; they do not establish TLR2-specific causality or effects on fertilizing competence.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 9","pages":"e70321"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0