Mangiferin Reduces Degeneration in Swine Preantral Follicles Enclosed in Ovarian Tissue Cultured In Vitro in the Presence of Doxorubicin.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2025-06-01 DOI:10.1111/rda.70085

Dax Ramyr E Silva Moreira, Gaby Judith Quispe Palomino, Isabor Sales Marinho Queiroz, Ana Flávia Bezerra da Silva, Anna Clara Accioly Ferreira, Benner Geraldo Alves, Selene Maia de Morais, Ariclécio Cunha de Oliveira, Ana Paula Ribeiro Rodrigues, Laritza Ferreira de Lima, José Ricardo de Figueiredo

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Abstract

This study assessed the potential protective effects of Mangiferin (MANGI) at various concentrations (10, 50 and 100 μM) on follicular survival and activation, enzyme activity of catalase (CAT) and superoxide dismutase (SOD), as well as the relative mRNA expression for BAX, BCL2 and SOD in porcine ovarian tissue cultured in vitro for 48 h with and without chemical stress induced by 0.3 μg/mL doxorubicin (DOX). The results indicated that when MANGI was associated with DOX, regardless of the tested concentration, MANGI increased (p < 0.05) follicle survival when compared to DOX alone, with only MANGI 10 + DOX and MANGI 50 + DOX treatments reducing follicular degeneration to levels comparable with the culture control. Regarding antioxidant enzymes, all treated groups decreased (p < 0.05) SOD activity compared to cultured control, while MANGI 10 treatment showed lower (p < 0.05) CAT activity compared to DOX treatment. Compared to cultured control, the mRNA levels of SOD were reduced (p < 0.05) in the MANGI 100, MANGI 10 + DOX and MANGI 100 + DOX treatments. In conclusion, supplementing the culture medium with MANGI attenuated the toxicity induced by DOX during the in vitro culture of pig preantral follicles enclosed in ovarian tissue.

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芒果苷减少阿霉素存在下体外培养的猪卵巢组织包围的猪腔前卵泡的变性。

在0.3 μg/mL多柔比星（DOX）诱导的体外培养48 h的猪卵巢组织中，研究了不同浓度（10 μM、50 μM和100 μM）芒果苷（mangiin， MANGI）对卵泡存活和活化、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）酶活性以及BAX、BCL2和SOD mRNA相对表达量的影响。结果表明，当MANGI与DOX相关联时，无论测试浓度如何，MANGI都增加(p

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.