Reproduction in Domestic Animals最新文献_第6页

Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain. 补编：西班牙动物生殖学会（AERA）第 17 届国际大会论文集，2024 年 10 月 17-19 日，西班牙马德里。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14697

引用次数: 0

Ethical aspects associated with genome alteration techniques applied to animal reproduction research. 与应用于动物繁殖研究的基因组改变技术有关的伦理问题。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14670

Lluis Montoliu

引用次数: 0

Efficient Method for Liposome-Based Gene Transfer in Cattle Embryos. 基于脂质体的牛胚胎基因转移的高效方法。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14730

Thays Antunes, Ana Beatriz Dos Reis Bartoli, Marcella Pecora Milazzotto

{"title":"Efficient Method for Liposome-Based Gene Transfer in Cattle Embryos.","authors":"Thays Antunes, Ana Beatriz Dos Reis Bartoli, Marcella Pecora Milazzotto","doi":"10.1111/rda.14730","DOIUrl":"https://doi.org/10.1111/rda.14730","url":null,"abstract":"In vitro embryo production (IVP) in cattle is crucial for advancing genetic enhancement and preserving valuable genetic lineages, enabling precise genetic modifications and gene studies through modern techniques. Successful genetic manipulation in cattle embryos requires efficient delivery of exogenous DNA/RNA molecules. This research investigates the efficacy of a single embryo culture system for developing genetically modified zona-free (ZF) embryos and examines the use of liposome-based SAMTOR target siRNA transfer in these individually cultured ZF embryos. The findings indicated that the individual culture system resulted in increased cleavage rates, and blastocyst rates were minimally impacted. The new culture system effectively achieved SAMTOR silencing, with 8-16 cell embryos exhibiting reduction in transcript levels compared to control. Measurement of total protein content in the spent culture media was performed to validate the single-culture approach for further analytical applications. Total protein content analysis demonstrated the system's suitability for comprehensive evaluation of the embryo-media interaction, enhancing the scope for in-depth genetic research and applications. This research sheds light into an innovative method to improve genetic editing techniques in reproduction research.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14730"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonality analysis on cryopreserved doses from the autochthonous cattle breeds Asturiana de la Montaña and Asturiana de los Valles. 对本土牛种 Asturiana de la Montaña 和 Asturiana de los Valles 的低温保存剂量进行季节性分析。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14599

Carolina Tamargo, Amer Salman, Estela Fernández-Alegre, Carmen Fueyo, Cristina Arija, Ángel Fernández, María J Merino, Felipe Martínez-Pastor, J Néstor Caamaño, Carlos O Hidalgo

{"title":"Seasonality analysis on cryopreserved doses from the autochthonous cattle breeds Asturiana de la Montaña and Asturiana de los Valles.","authors":"Carolina Tamargo, Amer Salman, Estela Fernández-Alegre, Carmen Fueyo, Cristina Arija, Ángel Fernández, María J Merino, Felipe Martínez-Pastor, J Néstor Caamaño, Carlos O Hidalgo","doi":"10.1111/rda.14599","DOIUrl":"https://doi.org/10.1111/rda.14599","url":null,"abstract":"Germplasm banking is a fundamental tool for the preservation of autochthonous breeds. Semen cryopreservation is effective for this task, but protocols are adapted to commercial species, and post-thawing sperm quality could be sensitive to environmental cues. We compared the post-thawing sperm quality in doses from the CBA-SERIDA bank in northern Spain for the Asturiana de la Montaña (AM) and Asturiana de los Valles (AV) autochthonous cattle breeds. Doses from 23 AM and 16 AV bulls (ejaculates from at least three different seasons) were assessed for motility (computer-assisted sperm analysis), physiology and chromatin status (flow cytometry) after thawing and after 5 h at 38°C. Data were analysed using linear mixed-effects and cosinor models for seasonal and breed effects and by correlations with the association of sperm quality with temperature-humidity index (THI), considering the interval of spermatogenesis plus maturation. The breed affected sperm quality, with higher motility for AV and higher apoptotic ratio, mitochondrial activity, reactive oxygen species, DNA fragmentation and chromatin immaturity for AM. However, seasonality effects were minimal, and THI was not associated with sperm quality. In summary, the season seems to be a minor factor in the post-thawing quality of the AM and AV autochthonous breeds, well-adapted to their local environment.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 3 ","pages":"e14599"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Why choose the rabbit to work in reproductive technology? 为什么选择兔子来从事生殖技术工作？

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14640

Francisco Marco-Jiménez, María Pilar Viudes-de-Castro, José Salvador Vicente

{"title":"Why choose the rabbit to work in reproductive technology?","authors":"Francisco Marco-Jiménez, María Pilar Viudes-de-Castro, José Salvador Vicente","doi":"10.1111/rda.14640","DOIUrl":"https://doi.org/10.1111/rda.14640","url":null,"abstract":"Rabbits have played a significant role in both livestock production and the advancement of reproductive scientific research. Their unique biological traits, including induced ovulation and a reproductive process that closely mirrors that of humans, have been pivotal in their use as a model. Moreover, their body size is perfectly aligned with the 3Rs principles: Replacement, Reduction, and Refinement. Consequently, techniques for gamete collection and embryo recovery, followed by their use in artificial insemination or embryo transfer, are characterized by being minimally invasive. However, refining in vitro fertilization and embryo culture techniques continues to present challenges. The incorporation of cutting-edge genomic editing tools, such as CRISPR/Cas9, has reestablished rabbits as essential models in genetic and biomedical research, driving scientific progress. This review aims to describe the most effective reproductive biotechnologies for both male and female rabbits and how these methodologies are in line with the 3Rs principles-Replacement, Reduction, and Refinement-highlighting their significance in conducting ethical research.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 3 ","pages":"e14640"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation of Plant-Based Freezing Extender With Cysteamine Preserves Quality Parameters and Fertility Potential of Buck Sperm During Cryopreservation Process. 在植物性冷冻扩展剂中添加半胱胺，可在冷冻过程中保持雄鹿精子的质量参数和生育潜力。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14724

Mahdi Mokhtari, Mahdi Khodaei-Motlagh, Mohammad Yahyaei, Reza Masoudi

{"title":"Supplementation of Plant-Based Freezing Extender With Cysteamine Preserves Quality Parameters and Fertility Potential of Buck Sperm During Cryopreservation Process.","authors":"Mahdi Mokhtari, Mahdi Khodaei-Motlagh, Mohammad Yahyaei, Reza Masoudi","doi":"10.1111/rda.14724","DOIUrl":"https://doi.org/10.1111/rda.14724","url":null,"abstract":"Sperm cryopreservation in small ruminant is an efficient strategy to distribute spermatozoa for reproductive programmes, but this process reduces the fertility potential of frozen-thawed spermatozoa. The aim of the current research was to evaluate the impact of different concentrations of cysteamine (CYS) in soybean lecithin (SL)-based medium on postthawed buck semen quality and fertility potential. Semen samples were collected from five bucks, twice a week, then diluted in the SL-based extender containing different concentrations of CYS as follows: extender containing 0 mM (control, C0), 1 mM (C1), 2 mM (C2), 4 mM (C4) and 8 mM (C8) CYS. Motility characteristics, membrane integrity, abnormal morphology, mitochondrial activity, acrosome integrity, viability, apoptotic-like changes, lipid peroxidation, DNA fragmentation, ROS concentration, pregnancy rate and kidding rate were evaluated after freeze-thaw process. In results, C1 resulted in greater (p ≤ 0.05) total motility, progressive motility, average path velocity, membrane integrity, mitochondrial activity, acrosome integrity, viability, pregnancy rate and kidding rate compared to the other groups. Furthermore, supplementation of freezing medium with 1 mM of CYS presented lower (p ≤ 0.05) apoptotic-like changes, lipid peroxidation, DNA fragmentation and ROS concentration compared to the other groups. On the other hand, C8 presented the least (p ≤ 0.05) total motility, progressive motility, average path velocity, membrane integrity, mitochondrial activity, acrosome integrity and viability as well as the highest (p ≤ 0.05) apoptotic-like changes, lipid peroxidation, DNA fragmentation and ROS concentration compared to the other groups. Therefore, supplementation of freezing medium with 1 mM CYS could be a helpful strategy to protect buck's spermatozoa quality and fertility potential during cryopreservation process.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14724"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Media viscosity affects post-thaw ram sperm rheotactic behaviour. 介质粘度影响公羊精子解冻后的流变行为。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14644

A Martín-Maestro, L Abril-Parreño, A J Soler, S Fair

引用次数: 0

A Novel Intravaginal Contraceptive Plug for Cats: A Preliminary Biocompatibility Assessment on Haematology and Vaginal Swab. 一种新型猫阴道内避孕塞：对血液学和阴道拭子的初步生物相容性评估

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14736

Sella Sofia Ainun, Muhammad Irham Bagus Santoso, Elma Yuliani Yessa, Gunanti, Ahmad Kafrawi Nasution, Syafiqah Saidin, Mokhamad Fakhrul Ulum

{"title":"A Novel Intravaginal Contraceptive Plug for Cats: A Preliminary Biocompatibility Assessment on Haematology and Vaginal Swab.","authors":"Sella Sofia Ainun, Muhammad Irham Bagus Santoso, Elma Yuliani Yessa, Gunanti, Ahmad Kafrawi Nasution, Syafiqah Saidin, Mokhamad Fakhrul Ulum","doi":"10.1111/rda.14736","DOIUrl":"https://doi.org/10.1111/rda.14736","url":null,"abstract":"This preliminary study evaluated the biocompatibility of a novel degradable intravaginal plug contraceptive composed of PEG 4000 and chitosan in cats using haematological profiling and vaginal cytology. Five healthy, non-pregnant female cats were fully anaesthetised and fitted with an intravaginal plug (10 × 0.3 mm) using an applicator, following oestrogen administration 3 h prior. Blood samples were collected from the cephalic vein on days 0 (pre-insertion) and 3 and 7 (post-insertion). Vaginal cytology examinations were conducted on day 0 (pre- and post-oestrogen injection) and days 1, 3 and 7 post-insertion. Haematological parameters, including red blood cell count, haemoglobin levels, haematocrit values, total white blood cell count and differentiation, showed no significant changes after contraceptive insertion (p > 0.05). Vaginal cytology indicated an acute inflammatory response in one out of five subjects on day three post-insertion. The distribution of vaginal epithelial cells (parabasal, intermediate and superficial) remained unaffected by contraception. Oestrogen injection resulted in the dominance of superficial cells up to day 7 of observation (p < 0.05). Overall, PEG 4000 and chitosan-based intravaginal plug contraceptives demonstrated sufficient biocompatibility, indicating their potential as viable contraceptive options for feline use.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14736"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of pseudopregnancy in bitch attending a veterinary teaching hospital in Spain. 在西班牙一家兽医教学医院就诊的母犬假孕发生率。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14638

Maria Victoria Falceto, Ana Maria Garrido, Marta Bueno, Maria Teresa Tejedor, Julia Cantin, Laura Garza-Moreno, Olga Mitjana

{"title":"Prevalence of pseudopregnancy in bitch attending a veterinary teaching hospital in Spain.","authors":"Maria Victoria Falceto, Ana Maria Garrido, Marta Bueno, Maria Teresa Tejedor, Julia Cantin, Laura Garza-Moreno, Olga Mitjana","doi":"10.1111/rda.14638","DOIUrl":"https://doi.org/10.1111/rda.14638","url":null,"abstract":"Pseudopregnancy (PSG) is one of the most common syndromes diagnosed after oestrous cycle in female dogs. We found a diagnosed prevalence of PSG at 30.81% among reproductive pathologies in bitch. Concentrated oestrous occurrences in spring and autumn influence PSG distribution. PSG onset is marked by behavioural changes, followed by physical signs (mammary enlargement and galactorrhea). The last oestrous-PSG onset interval ranged from 1 to 6 months (median = 3.0 months) and the median for the interval spaying-PSG onset was 7.0 days. Half of the cases were discharged after 16 days, surpassing recommended treatment periods for cabergoline (4-6 days) and metergoline (8 days). Although Elizabethan collars were recommended, actual compliance stood at two-thirds of cases. Our study highlights the possible underestimation of the PSG prevalence, probably due to lack of identification of clinical signs by owners. Further research is warranted to better understand possible risk factors, preventive measures or therapeutic options.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 Suppl 3 ","pages":"e14638"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the Application of PGF_2α Associated With Ovulation Induction in a Fixed-Time Superovulation Programme for Precocious Nellore Heifers. 在针对早熟内洛尔母牛的固定时间超排卵计划中，应用 PGF2α 与排卵诱导相关的效果。

IF 1.6 3区农林科学

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI: 10.1111/rda.14734

Tamires Korchovei Sanches, Pamela Mara Celestino Soares, Marcio de Oliveira Marques, Rubens Cesar Pinto da Silva, Luiz Francisco Machado Pfeifer, Amanda Fonseca Zangirolamo, Fábio Morotti, Marcelo Marcondes Seneda

{"title":"Effect of the Application of PGF2α Associated With Ovulation Induction in a Fixed-Time Superovulation Programme for Precocious Nellore Heifers.","authors":"Tamires Korchovei Sanches, Pamela Mara Celestino Soares, Marcio de Oliveira Marques, Rubens Cesar Pinto da Silva, Luiz Francisco Machado Pfeifer, Amanda Fonseca Zangirolamo, Fábio Morotti, Marcelo Marcondes Seneda","doi":"10.1111/rda.14734","DOIUrl":"https://doi.org/10.1111/rda.14734","url":null,"abstract":"This study evaluated the effect of prostaglandin F2α (PGF2α) associated with gonadotropin-releasing hormone (GnRH) for ovulation induction in precocious indicus heifers submitted to a fixed-time superovulation (SOV) programme. Precocious Nellore heifers (n = 35), aged 13 months, were subjected to the SOV protocol. On day 0 (D0), all animals received intravaginal insertion of a progesterone (P4) device along with intramuscular administration of 2 mg of oestradiol benzoate, plus 200 IU of follicle-stimulating hormone in decreasing doses, with 12-h intervals between D4 and D7, in addition to 150 μg of D-cloprostenol on D6 and device removal on D7. On D8, the donors received 10.5 μg of buserelin acetate and the treatment group received 300 μg of D-cloprostenol/PGF2α. Artificial insemination was performed 12 h and 24 h after GnRH administration using frozen semen. On D15 of the protocol (i.e., D7 after insemination), the embryos were collected and evaluated. All animals passed through the control and treatment groups. Results were evaluated by analysis of variance using an adjusted mixed-effects model (p < 0.05). There was no difference in the total number of embryos between the control and treatment groups (10.40 ± 1.52 vs. 9.60 ± 1.36; p = 0.63) or viable embryos (6.30 ± 1.22 vs. 4.30 ± 0.71). For precocious indicus heifers, treatment with PGF2α in association with GnRH did not affect embryo production in the fixed-time SOV protocol.","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"59 10","pages":"e14734"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0