Reproduction in Domestic Animals最新文献

{"title":"Special Issue: Proceedings of the Joint 29th Annual European Society for Domestic Animal Reproduction (ESDAR) Conference & 4th European College of Animal Reproduction (ECAR) Symposium, 17-19 September 2026, Olsztyn, Poland.","authors":"","doi":"10.1111/rda.70272","DOIUrl":"https://doi.org/10.1111/rda.70272","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 2 ","pages":"e70272"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to 'Exploring the Landscape of Circular RNAs in Testes of Dezhou Donkey During Distinct Developmental Stages'.","authors":"","doi":"10.1111/rda.70316","DOIUrl":"10.1111/rda.70316","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 9","pages":"e70316"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13536531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Proceedings of the Joint 29th Annual European Society for Domestic Animal Reproduction (ESDAR) Conference & 4th European College of Animal Reproduction (ECAR) Symposium, 17-19 September 2026, Olsztyn, Poland.","authors":"","doi":"10.1111/rda.70275","DOIUrl":"https://doi.org/10.1111/rda.70275","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 2 ","pages":"e70275"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial.","authors":"Wojciech Barański","doi":"10.1111/rda.70278","DOIUrl":"10.1111/rda.70278","url":null,"abstract":"","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 Suppl 2 ","pages":"e70278"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13538266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Oocyte Ultrastructure and Antioxidant Defence System of In Vitro Matured Buffalo Oocytes via Resveratrol Supplementation.","authors":"Anwar M M El-Mahdy, Laila Bakeer, A A El-Darawany, Sherif M Shamiah, Emad A Abdallah, Khaled M Al-Marakby, Mustafa Shukry, Sameh Abdelnour","doi":"10.1111/rda.70319","DOIUrl":"10.1111/rda.70319","url":null,"abstract":"<p><p>Despite buffalo's ability to thrive in challenging climates, the scarcity of superior germplasm remains a significant barrier to enhancing the global buffalo industry. Resveratrol (RE) is a potent antioxidant known to improve oocyte maturation and developmental competence in several mammalian species. Yet, its specific impact on the ultrastructural integrity and antioxidant defence systems of buffalo oocytes remain to be fully elucidated. Cumulus-oocyte complexes (COCs) were cultured during In Vitro maturation (IVM) with varying concentrations of resveratrol (0, 0.5, 0.75, or 1 μM) and designated as the CON, RE0.5, RE0.75, and RE1.0 groups, respectively. Following IVM, maturation rates and ultrastructural integrity were assessed, and the levels of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) were quantified. The results indicated that the RE0.5 group exhibited a significantly higher metaphase II (MII) rate than the CON group (p < 0.05), whereas the RE0.75 and RE1.0 groups did not differ significantly compared with other groups (p > 0.05). Furthermore, supplementing the maturation media with RE (0.5, 0.75, or 1 μM) significantly increased the percentage of fully expanded cumulus complexes (p < 0.05). This was accompanied by a significant reduction in the rates of partially expanded, slightly expanded, and non- expanded cumulus complexes compared with the control treatment (p < 0.05). The RE0.5 group showed the lowest MDA levels and the highest CAT levels compared with the other groups (p < 0.05). Additionally, the RE0.5 group exhibited significantly higher GSH levels than the other treatment groups (p < 0.05). The SOD levels were decreased in all RE groups (p < 0.05) compared with control group. Ultrastructural analysis revealed that supplementation with 0.5 or 0.75 μM RE preserved the morphological integrity of the mitochondria, the zona pellucida, a dense ooplasm, and a well-organized distribution of cortical granules and short microvilli. These improvements were characterized by a normal perivitelline space and the successful extrusion of the first polar body, in contrast to the structural alterations observed in the CON group. In conclusion, supplementation with RE (0.5 or 0.75 μM) maintained the ultrastructural integrity of buffalo oocytes during IVM. This preservation is likely attributable to the potent antioxidant activity of resveratrol, which effectively mitigates oxidative stress, thereby enhancing both the cytoplasmic and nuclear maturation of buffalo oocytes In Vitro.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 9","pages":"e70319"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148970987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Sildenafil Citrate for the Treatment of Erectile Dysfunction in Stallions: First Case Reports.","authors":"Thaís Mendes Sanches Cavalero, Frederico Ozanam Papa, Gabriel Augusto Monteiro, Patrícia de Mello Papa, Felipe Pires Hartwig, Camila de Paula Freitas-Dell'Aqua, Marco Antônio Alvarenga, Marcela Souza E Freitas, Yame Fabres Robaina Sancler-Silva","doi":"10.1111/rda.70318","DOIUrl":"10.1111/rda.70318","url":null,"abstract":"<p><p>Pharmacological aids can be useful alternatives to enhance penile erection and/or improve ejaculatory function, thereby prolonging the reproductive life of stallions with ejaculatory disorders. This study aimed to evaluate the effects of sildenafil citrate in the treatment of erectile dysfunction and on semen quality in two aged stallions, a Quarter Horse (18 years) and a Mangalarga Marchador (25 years), presented to the Veterinary Hospital of FMVZ-UNESP, Botucatu, SP, Brazil. The treatment consisted of oral administration of sildenafil citrate at 0.2 mg/kg. Thirty minutes after administration, the Quarter Horse stallion achieved full erection and ejaculated in all collection attempts (100%; 3/3), with semen parameters within normal ranges for the species. The Mangalarga Marchador showed a partial response to treatment, with successful ejaculation in only one of three attempts (33.3%) and exhibited oligozoospermia, possibly associated with previously identified age-related testicular degeneration concurrent with the erectile dysfunction. To the best of the authors' knowledge, this is the first report on the use of sildenafil citrate for managing erectile dysfunction in stallions, demonstrating its therapeutic potential. However, the results suggest individual variability in response to treatment, indicating the need for additional controlled studies to establish more precise dose-response protocols.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 9","pages":"e70318"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13551068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Anethole on Meiotic Resumption and Oxidative Balance During in Vitro Maturation of Ovine Oocytes.","authors":"Anderson Nascimento de Andrade, Solano Dantas Martins, Alesandro Silva Ferreira, Naiza Arcângela Ribeiro de Sá, Jonathan Elias Rodrigues Martins, Daniela Ribeiro Alves, Selene Maia de Morais, José Henrique Leal-Cardoso, Luciana Rocha Faustino, Vânia Marilande Cecatto, Valdevane Rocha Araújo","doi":"10.1111/rda.70296","DOIUrl":"10.1111/rda.70296","url":null,"abstract":"<p><p>The aim of this study was to evaluate the effect of anethole addition to the ovine oocyte in vitro maturation (IVM) medium on viability and chromatin configuration, intracellular production of reactive oxygen species (ROS), enzymatic activity of superoxide dismutase (SOD) and catalase (CAT), as well as the levels of reduced thiols in IVM medium, in addition to its antioxidant potential (DPPH and ABTS). Cumulus-oocyte complexes (COCs) were randomly matured in IVM medium in the absence (control group, CTRL) or presence of anethole at concentrations of 30 (AN30), 300 (AN300), and 2000 μg/mL (AN2000). COCs were matured in groups under mineral oil at 39°C in a humidified atmosphere containing 5% CO₂ in air for 24 h. At the end of IVM, part of the COCs was used to assess viability and chromatin configuration using Hoechst 33,342. The remaining COCs were used to assess intracellular ROS levels using the fluorescent probe H2DCFDA. Furthermore, the IVM medium was collected to evaluate antioxidant status (SOD, CAT, and reduced thiols). Finally, the antioxidant potential of different anethole concentrations was evaluated using DPPH and ABTS assays. It was observed that AN300 promoted higher meiotic resumption rates compared to the other treatments (p < 0.05) and a lower rate of oocyte degeneration compared to AN30 (p < 0.05). However, regarding the proportion of oocytes at MII, it was observed that only AN2000 was lower than AN300 (p < 0.05). Moreover, AN30 resulted in lower ROS levels compared to AN300 and AN2000 (p < 0.05). SOD activity was lower in AN2000 compared to the other treatments (p < 0.05). AN2000 also showed the highest antioxidant capacity in the DPPH and ABTS assays among the anethole concentrations, although it was lower than the control group. In conclusion, anethole supplementation during IVM positively affected oocyte meiotic progression in sheep.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70296"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13439492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Long-Acting Progesterone and Intravaginal Device Priming Protocols for Inducing Ovarian Activity in Dromedary Camels.","authors":"Mahmoud Moussa, Salahaddin Ahmed, Mohamed Elbaz, Kamaal Pasha","doi":"10.1111/rda.70312","DOIUrl":"10.1111/rda.70312","url":null,"abstract":"<p><p>The objectives of this study were to evaluate the efficacy of long-acting progesterone (P4) versus a P4-releasing intravaginal device (PRID) in restoring ovarian activity in dromedary camels with inactive ovaries, and to determine whether P4 priming is necessary before eCG administration. Seventy-five camels were equally assigned to three groups: a control group receiving only 1500 IU eCG; a PRID group treated with a 12-day PRIDΔ followed by eCG; and a P4 long-acting (P4 LA) group administered 1500 mg P4 LA followed by eCG on Day 12. Serum P4 and estradiol (E2) levels were measured on Days 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, and 20, and reproductive outcomes were recorded. Results showed that the P4 LA group exhibited a rapid increase followed by a sharp decline in P4 levels, whereas the PRID group maintained more stable P4 levels, with a gradual decrease by Day 12. Moreover, from Day 16 onward, primed groups had significantly higher E2 levels than controls (p < 0.05). The response rate was 100% in the primed groups versus 60% in controls (p < 0.05), and the interval from eCG injection to ovulatory follicle development was shorter in primed groups (p < 0.05). The 18-day conception rate was highest in the PRID group (100%), followed by P4 LA (84%) and controls (53.3%). However, the pregnancy rate at Day 60 did not differ between PRID (84%) and P4 LA (80%) groups, both of which were significantly higher than controls (13.3%) (p < 0.05). In conclusion, P4 priming is essential before eCG administration, and LA P4 offers practical advantages for field veterinarians.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70312"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Follicular Development and Luteal Function During Breeding and Non-Breeding Seasons in Subtropical Beetal Goats.","authors":"Amjad Atta, Muhammad Irfan-Ur-Rehman Khan, Abdul Rehman, Kamran Ashraf, Rehana Kausar","doi":"10.1111/rda.70292","DOIUrl":"10.1111/rda.70292","url":null,"abstract":"<p><p>Reproductive seasonality in subtropical goats is considered weak, yet seasonal differences in ovarian dynamics remain poorly characterized. This study compared ovarian follicular and luteal dynamics during breeding (September-November) and non-breeding (May-July) seasons in six multiparous Beetal goats monitored for three consecutive estrous cycles per season, (i.e., 18 cycles per season) and maintained under semi-intensive conditions. Ovarian activity in each goat was monitored daily for three consecutive cycles using transrectal ultrasonography, and progesterone concentrations were analysed to evaluate follicular wave patterns, ovulation and luteal function. The distribution of cycle types did not differ significantly between seasons (p > 0.05); however, normal cycles occurred more frequently during the breeding season as a non-significant trend (p = 0.08). Inter-ovulatory intervals were longer, and multi-wave patterns were more frequent during the non-breeding season (p < 0.05). Preovulatory follicle size (p = 0.66) and growth rate (p = 0.18) were unaffected by season, indicating conserved follicular characteristics. Corpus luteum diameter was influenced by cycle type but not season, whereas progesterone concentrations showed a significant season-by-stage interaction (p = 0.04), indicating that seasonal differences were not uniform across the luteal cycle. Double ovulations occurred only during the breeding season. These findings indicate weak but measurable seasonal modulation of ovarian function in subtropical goats, providing insights for improving reproductive management strategies.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70292"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13425417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Dynamics of Ovine Allantoic and Amniotic Fluids During Gestation.","authors":"Viviane Maria Codognoto, Rogério Araújo de Almeida Filho, Marcos Gomides Carvalho, Paula Zanin Rates, Flávio Augusto Lourencetti, Laiza Sartori de Camargo, Claudio Willian Delfes de Camargo, Juanita Valentina Guzmán Martínez, Luan Sitó da Silva, Eunice Oba","doi":"10.1111/rda.70300","DOIUrl":"10.1111/rda.70300","url":null,"abstract":"<p><p>Gestational success in ruminants depends on the maintenance of a dynamic maternal-foetal microenvironment, resulting from complex interactions among the conceptus, extraembryonic membranes, maternal tissues, and gestational fluids. This study performed a comparative and longitudinal proteomic characterization of allantoic and amniotic fluids throughout pregnancy in sheep, aiming to elucidate the molecular mechanisms involved in maternal-foetal communication and embryonic development. Samples of allantoic and amniotic fluids aseptically collected from extraembryonic compartments after uterine opening following animal slaughter and allocated into three gestational groups according to foetal development: early gestation (0-50 days; n = 6), mid gestation (50-100 days; n = 3) and late gestation (100-150 days; n = 5). After centrifugation and protein quantification using the Bradford method, standardized aliquots were subjected to in-solution protein digestion and analysed by nano-LC-ESI-Q-TOF mass spectrometry. A total of 201 proteins were identified in the allantoic fluid and 159 proteins in the amniotic fluid, with five proteins showing significant differences across gestational stages. In the allantoic fluid, differentially abundant proteins included alpha-2-macroglobulin, gelsolin, basement membrane-specific heparan sulphate proteoglycan core protein and alpha-globin chain. In the amniotic fluid, changes were observed in peroxiredoxin-2, mucin-5 AC, fetuin-B, cystatin domain-containing protein and globin domain-containing protein. Multivariate analyses revealed progressive remodelling of the foetal extraembryonic microenvironment, associated with proteolytic control, tissue remodelling, redox homeostasis, epithelial protection and metabolic processes. Collectively, these findings reinforce the active role of foetal extraembryonic fluids in gestational physiology and highlight their potential as sources of biomarkers associated with reproductive success in sheep.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"61 8","pages":"e70300"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13445540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148679733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}