Chlorogenic Acid Increases Follicle Survival, Stromal Cell Density, Levels of Thiol and Glutathione Peroxidase Activity in Cultured Bovine Ovarian Tissues.

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Pedro A A Barroso, Venância A N Azevedo, Danisvânia R Nascimento, Ernando I T de Assis, Miguel F De Lima Neto, Alesandro S Ferreira, Solano D Martins, Anderson W B Silva, Valdevane R Araújo, José R V Silva
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引用次数: 0

Abstract

This study aimed to investigate the radical scavenging activity and the effects of chlorogenic acid on follicular survival and development, stromal cell density and collagen fibres distribution in extracellular matrix, as well as on mRNA expression of nuclear factor erythroid 2-related factor 2 (NRF2), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and periredoxin 6 (PRDX6), thiol levels, and activity of SOD, CAT, GPX enzymes in cultured bovine ovarian tissues. The free radical scavenging potential of chlorogenic acid was assessed through DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays. The ovarian fragments were cultured in α-MEM+ alone or supplemented with 25, 50, 100, and 200 μmol/L chlorogenic acid for 6 days at 38.5°C and 5% CO2. Follicular growth and survival, stromal cell density, and collagen fibres were analysed by classical histology. Expression of mRNA for NRF2, SOD, CAT, GPX, and PRDX6 was evaluated by real-time PCR. Thiol levels and activity of SOD, CAT, GPX were investigated by Bradford method. The results showed that the presence of 100 μmol/L chlorogenic acid in culture medium promotes an increase in morphologically normal follicles and stromal cell density when compared to tissues cultured in control medium. Chlorogenic acid also increased thiol levels and GPX activity, but did not influence the distribution of collagen fibres or the expression of the analysed mRNAs. In conclusion,100 μmol/L chlorogenic acid increases the percentage of morphologically normal follicles, stromal cell density, thiol levels, and GPX activity in bovine ovarian tissues cultured in vitro.

绿原酸提高培养牛卵巢组织中卵泡存活率、基质细胞密度、硫醇和谷胱甘肽过氧化物酶活性水平。
本研究旨在探讨绿原酸对体外培养的牛卵巢组织中卵泡存活发育、基质细胞密度、细胞外基质胶原纤维分布、核因子-红系2相关因子2 (NRF2)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)、环氧还蛋白6 (PRDX6) mRNA表达、硫醇水平及SOD、CAT、GPX酶活性的影响。通过DPPH(2,2-二苯基-1-picrylhydrazyl)和ABTS (2,2'- azno -bis(3-乙基苯并噻唑-6-磺酸))测定绿原酸对自由基的清除能力。卵巢碎片分别在α-MEM+中单独或添加25、50、100和200 μmol/L绿原酸,在38.5℃、5% CO2条件下培养6 d。通过经典组织学分析毛囊生长和存活、基质细胞密度和胶原纤维。实时荧光定量PCR检测NRF2、SOD、CAT、GPX、PRDX6 mRNA的表达。采用Bradford法检测硫醇水平及SOD、CAT、GPX活性。结果表明,与对照培养基相比,100 μmol/L绿原酸能促进形态正常的卵泡和基质细胞密度的增加。绿原酸也增加了硫醇水平和GPX活性,但不影响胶原纤维的分布或分析mrna的表达。综上所述,100 μmol/L绿原酸可提高体外培养牛卵巢组织形态正常卵泡百分率、基质细胞密度、硫醇水平和GPX活性。
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来源期刊
Reproduction in Domestic Animals
Reproduction in Domestic Animals 农林科学-奶制品与动物科学
CiteScore
3.00
自引率
5.90%
发文量
238
审稿时长
4-8 weeks
期刊介绍: The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.
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