姜黄素油纳米乳对水牛精子冷冻保存的影响:精子质量、运动参数、精子超微结构、氧化应激、细胞凋亡、微生物群、分子对接分析和受精能力。

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Wael A Khalil, Mahmoud A E Hassan, Mohamed M Hegazy, Khaled Fathy, Mostafa A El-Harairy, Aya A Ismail, Mohammed A Alfattah, Ramya Ahmad Sindi, Sameh A Abdelnour
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引用次数: 0

摘要

本研究探讨了姜黄素油纳米乳液(CONE)在基于tris的冷冻扩展剂中的应用,以减轻冷冻损伤效应,提高水牛精子的抗冻能力。将5头可育水牛公牛(n = 40次射精)的精液进行分离,分别添加0.5%锥形(CONE0.5)、1%锥形(CONE1)或不含锥形(CONE0,对照),然后进行扩展和包装。解冻后的评估包括精子运动学参数、氧化应激标志物、细胞凋亡水平、微生物负荷、超微结构完整性、分子对接相互作用和受孕率。与CONE0和CONE1组相比,CONE0.5组的精子活力、进行性运动和膜完整性显著提高,一氧化氮和丙二醛水平显著降低(p 0.05)。分子对接分析显示,姜黄素与caspase-3 (-7.87 kcal/mol)、HSP70 (-8.52 kcal/mol)和PRDX-1 (-8.90 kcal/mol)具有较强的结合亲和力,提示其作用机制。综上所述,在水牛精液冷冻培养基中加入0.5%的CONE作为冷冻保护添加剂,可以改善解冻后精液的质量参数,表明可能提高生殖效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Curcumin Oil Nano-Emulsion on Buffalo Sperm Cryopreservation: Insights on Sperm Quality, Kinematic Parameters, Sperm Ultrastructure, Oxidative Stress, Apoptosis, Microbiota, and Molecular Docking Analysis and Fertilising Capacity.

This study investigated the potential of incorporating curcumin oil nano-emulsion (CONE) into a Tris-based freezing extender to relieve cryodamage effects and enhance sperm cryo-resistance in buffalo bulls. Pooled semen from five fertile buffalo bulls (n = 40 ejaculates) was divided and supplemented with 0.5% CONE (CONE0.5), 1% CONE (CONE1), or without CONE (CONE0, control), then extended and packaged. Post-thaw assessments included sperm kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, and conception rates. The CONE0.5 group exhibited significantly higher sperm viability, progressive motility, and membrane integrity, as well as significantly lower nitric oxide and MDA levels, compared to the CONE0 and CONE1 groups (p < 0.05), indicating reduced oxidative stress. Compared to the CONE0 group, the CONE0.5 group exhibited a significantly higher percentage of viable and early apoptotic sperm, and a significantly lower percentage of late apoptotic sperm (p < 0.05). Both CONE-treated groups demonstrated significantly lower post-thaw total bacterial, spore-forming bacterial, and coliform bacterial counts (p < 0.05). Conception rates were higher in the CONE0.5 group (80.0%) compared to the CONE0 group (68.0%) (p > 0.05). Molecular docking analysis revealed strong binding affinities between curcumin and caspase-3 (-7.87 kcal/mol), HSP70 (-8.52 kcal/mol), and PRDX-1 (-8.90 kcal/mol), suggesting potential mechanisms of action. In summary, the inclusion of 0.5% CONE as a cryoprotective additive in buffalo semen freezing media demonstrates a potential for improving post-thaw semen quality parameters, suggesting a possible enhancement of reproductive efficiency.

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来源期刊
Reproduction in Domestic Animals
Reproduction in Domestic Animals 农林科学-奶制品与动物科学
CiteScore
3.00
自引率
5.90%
发文量
238
审稿时长
4-8 weeks
期刊介绍: The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.
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