Wael A Khalil, Mahmoud A E Hassan, Mohamed M Hegazy, Khaled Fathy, Mostafa A El-Harairy, Aya A Ismail, Mohammed A Alfattah, Ramya Ahmad Sindi, Sameh A Abdelnour
{"title":"姜黄素油纳米乳对水牛精子冷冻保存的影响:精子质量、运动参数、精子超微结构、氧化应激、细胞凋亡、微生物群、分子对接分析和受精能力。","authors":"Wael A Khalil, Mahmoud A E Hassan, Mohamed M Hegazy, Khaled Fathy, Mostafa A El-Harairy, Aya A Ismail, Mohammed A Alfattah, Ramya Ahmad Sindi, Sameh A Abdelnour","doi":"10.1111/rda.70062","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the potential of incorporating curcumin oil nano-emulsion (CONE) into a Tris-based freezing extender to relieve cryodamage effects and enhance sperm cryo-resistance in buffalo bulls. Pooled semen from five fertile buffalo bulls (n = 40 ejaculates) was divided and supplemented with 0.5% CONE (CONE0.5), 1% CONE (CONE1), or without CONE (CONE0, control), then extended and packaged. Post-thaw assessments included sperm kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, and conception rates. The CONE0.5 group exhibited significantly higher sperm viability, progressive motility, and membrane integrity, as well as significantly lower nitric oxide and MDA levels, compared to the CONE0 and CONE1 groups (p < 0.05), indicating reduced oxidative stress. Compared to the CONE0 group, the CONE0.5 group exhibited a significantly higher percentage of viable and early apoptotic sperm, and a significantly lower percentage of late apoptotic sperm (p < 0.05). Both CONE-treated groups demonstrated significantly lower post-thaw total bacterial, spore-forming bacterial, and coliform bacterial counts (p < 0.05). Conception rates were higher in the CONE0.5 group (80.0%) compared to the CONE0 group (68.0%) (p > 0.05). Molecular docking analysis revealed strong binding affinities between curcumin and caspase-3 (-7.87 kcal/mol), HSP70 (-8.52 kcal/mol), and PRDX-1 (-8.90 kcal/mol), suggesting potential mechanisms of action. In summary, the inclusion of 0.5% CONE as a cryoprotective additive in buffalo semen freezing media demonstrates a potential for improving post-thaw semen quality parameters, suggesting a possible enhancement of reproductive efficiency.</p>","PeriodicalId":21035,"journal":{"name":"Reproduction in Domestic Animals","volume":"60 4","pages":"e70062"},"PeriodicalIF":1.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of Curcumin Oil Nano-Emulsion on Buffalo Sperm Cryopreservation: Insights on Sperm Quality, Kinematic Parameters, Sperm Ultrastructure, Oxidative Stress, Apoptosis, Microbiota, and Molecular Docking Analysis and Fertilising Capacity.\",\"authors\":\"Wael A Khalil, Mahmoud A E Hassan, Mohamed M Hegazy, Khaled Fathy, Mostafa A El-Harairy, Aya A Ismail, Mohammed A Alfattah, Ramya Ahmad Sindi, Sameh A Abdelnour\",\"doi\":\"10.1111/rda.70062\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the potential of incorporating curcumin oil nano-emulsion (CONE) into a Tris-based freezing extender to relieve cryodamage effects and enhance sperm cryo-resistance in buffalo bulls. Pooled semen from five fertile buffalo bulls (n = 40 ejaculates) was divided and supplemented with 0.5% CONE (CONE0.5), 1% CONE (CONE1), or without CONE (CONE0, control), then extended and packaged. Post-thaw assessments included sperm kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, and conception rates. The CONE0.5 group exhibited significantly higher sperm viability, progressive motility, and membrane integrity, as well as significantly lower nitric oxide and MDA levels, compared to the CONE0 and CONE1 groups (p < 0.05), indicating reduced oxidative stress. Compared to the CONE0 group, the CONE0.5 group exhibited a significantly higher percentage of viable and early apoptotic sperm, and a significantly lower percentage of late apoptotic sperm (p < 0.05). Both CONE-treated groups demonstrated significantly lower post-thaw total bacterial, spore-forming bacterial, and coliform bacterial counts (p < 0.05). Conception rates were higher in the CONE0.5 group (80.0%) compared to the CONE0 group (68.0%) (p > 0.05). Molecular docking analysis revealed strong binding affinities between curcumin and caspase-3 (-7.87 kcal/mol), HSP70 (-8.52 kcal/mol), and PRDX-1 (-8.90 kcal/mol), suggesting potential mechanisms of action. In summary, the inclusion of 0.5% CONE as a cryoprotective additive in buffalo semen freezing media demonstrates a potential for improving post-thaw semen quality parameters, suggesting a possible enhancement of reproductive efficiency.</p>\",\"PeriodicalId\":21035,\"journal\":{\"name\":\"Reproduction in Domestic Animals\",\"volume\":\"60 4\",\"pages\":\"e70062\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproduction in Domestic Animals\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/rda.70062\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction in Domestic Animals","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/rda.70062","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Effects of Curcumin Oil Nano-Emulsion on Buffalo Sperm Cryopreservation: Insights on Sperm Quality, Kinematic Parameters, Sperm Ultrastructure, Oxidative Stress, Apoptosis, Microbiota, and Molecular Docking Analysis and Fertilising Capacity.
This study investigated the potential of incorporating curcumin oil nano-emulsion (CONE) into a Tris-based freezing extender to relieve cryodamage effects and enhance sperm cryo-resistance in buffalo bulls. Pooled semen from five fertile buffalo bulls (n = 40 ejaculates) was divided and supplemented with 0.5% CONE (CONE0.5), 1% CONE (CONE1), or without CONE (CONE0, control), then extended and packaged. Post-thaw assessments included sperm kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, and conception rates. The CONE0.5 group exhibited significantly higher sperm viability, progressive motility, and membrane integrity, as well as significantly lower nitric oxide and MDA levels, compared to the CONE0 and CONE1 groups (p < 0.05), indicating reduced oxidative stress. Compared to the CONE0 group, the CONE0.5 group exhibited a significantly higher percentage of viable and early apoptotic sperm, and a significantly lower percentage of late apoptotic sperm (p < 0.05). Both CONE-treated groups demonstrated significantly lower post-thaw total bacterial, spore-forming bacterial, and coliform bacterial counts (p < 0.05). Conception rates were higher in the CONE0.5 group (80.0%) compared to the CONE0 group (68.0%) (p > 0.05). Molecular docking analysis revealed strong binding affinities between curcumin and caspase-3 (-7.87 kcal/mol), HSP70 (-8.52 kcal/mol), and PRDX-1 (-8.90 kcal/mol), suggesting potential mechanisms of action. In summary, the inclusion of 0.5% CONE as a cryoprotective additive in buffalo semen freezing media demonstrates a potential for improving post-thaw semen quality parameters, suggesting a possible enhancement of reproductive efficiency.
期刊介绍:
The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques.
Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings.
Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.