将酿酒葡萄渣作为改善公猪精液质量的膳食补充剂

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Armando Quintero-Moreno, Cristina Martínez-López, Chiara Luongo, María Luz García, María José Argente, Gema Romero, Cristina Pérez-Patiño, José Ramón Díaz, Francisco A García-Vázquez, Pedro José Llamas-López
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引用次数: 0

摘要

公猪精子由于含有大量不饱和脂肪酸,极易受到氧化损伤,而不饱和脂肪酸很容易被活性氧(ROS)破坏。过量的 ROS 会导致脂质过氧化、DNA 断裂和酶活性受损,最终降低精子质量和繁殖能力。酿酒葡萄渣(WGP)是酿酒过程中产生的副产品,富含多酚,包括类黄酮(花青素和槲皮素)、二苯乙烯类化合物(白藜芦醇)和单宁,具有很强的抗氧化性。本研究旨在评估膳食中补充 4% WGP 对公猪射精量和精子储存期间质量的影响。研究人员将 20 头公猪分为两组:对照组和补充 4% WGP 的 WGP 组,前者饲喂标准日粮,后者饲喂 4 个月。收集精液样本,并在冷藏保存 1、3 和 5 天后分析每毫升精液中的精子数量、总抗氧化能力(精浆中)和质量参数(活力、运动参数、线粒体活性、顶体完整性、存活率)。结果显示,与对照组(P 0.05)和冷藏储存组(P > 0.05)相比,补充 WGP 增加了每毫升精子的数量。精子浓度的提高可增加每头公猪每年生产的剂量,从而提高猪精液生产的利润。鉴于 WGP 的成本较低(10.03 欧元/公猪/年),该策略可为提高公猪繁殖性能提供一种经济有效的方法。这些发现支持进一步研究优化 WGP 的添加水平,并探索其对公猪繁殖力和繁殖效率的广泛影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Wine Grape Pomace as a Dietary Supplement to Improve Semen Quality in Boars.

Boar spermatozoa are highly susceptible to oxidative damage due to their high content of unsaturated fatty acids, which are prone to disruption by reactive oxygen species (ROS). Excessive ROS can induce lipid peroxidation, DNA fragmentation and impaired enzyme activity, ultimately reducing sperm quality and reproductive performance. Wine grape pomace (WGP), a by-product of the winemaking process, is rich in polyphenols, including flavonoids (anthocyanins and quercetin), stilbenes (resveratrol) and tannins, which possess strong antioxidant properties. This study aimed to evaluate the effects of dietary supplementation with 4% WGP on boar ejaculate output and sperm quality during storage. Twenty boars were divided into two groups: a control group fed a standard diet and a WGP group supplemented with 4% WGP for 4 months. Semen samples were collected and analysed for ejaculate number of sperm per ml, total antioxidant capacity (in seminal plasma) and quality parameters (motility, kinematic parameters, mitochondrial activity, acrosome integrity, viability) after 1, 3 and 5 days of refrigerated storage. Results showed that WGP supplementation increased the number of sperm per ml compared to the control group (p < 0.05), resulting in approximately two additional seminal doses per ejaculate, without negatively affecting other seminal parameters (p > 0.05) and refrigeration storage (p > 0.05). This improvement in sperm concentration could enhance the profitability of swine semen production by increasing the number of doses produced per boar annually. Given the low cost of WGP (10.03 €/boar/year), this strategy could offer a cost-effective approach to improving reproductive performance in boars. These findings support further research into optimising WGP inclusion levels and exploring its broader impacts on boar fertility and reproductive efficiency.

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来源期刊
Reproduction in Domestic Animals
Reproduction in Domestic Animals 农林科学-奶制品与动物科学
CiteScore
3.00
自引率
5.90%
发文量
238
审稿时长
4-8 weeks
期刊介绍: The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.
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