New Insights in Bovine Follicular Cysts.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2025-03-01 DOI:10.1111/rda.70048

Alice Carbonari, Nicola Antonio Martino, Matteo Burgio, Vincenzo Cicirelli, Lorenza Frattina, Maria Elena Dell'Aquila, Annalisa Rizzo

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引用次数: 0

Abstract

The aim of the present study was to understand the involved factors in follicular cysts in dairy cows. The study consisted of an in vivo and in vitro approach. The in vivo part, hormonal evaluation (Kisspeptin-10 [Kp-10], Gonadotropin inhibiting hormone [GnIH], Luteinizing hormone [LH], Oestrogens [E₂] and cortisol) was performed in sera of both healthy (H) and cows with follicular cysts (FC). The in vitro part was concentrated on estimating the distribution of GPR54 (Kp-10 receptor) and GPR147 (GnIH receptor) on cystic and preovulatory follicles. Serum concentrations of Kp-10, GnIH, LH, E₂ and cortisol were significantly higher in Group FC compared with Group H. Gene expression analysis showed a reduction in GPR54 mRNA levels in FC compared to preovulatory follicles, while no expression of the GPR147 receptor was detected. The lower presence of GPR54 in FC compared to the preovulatory follicle can be determined by a down-receptor regulation induced by elevated serum concentrations of Kp-10 in cows with ovarian FC. Endocrine imbalances of the hypothalamic-pituitary-ovarian axis, characterising FC, may reflect altered patterns of Kp-10 and GnIH secretion.

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牛卵泡囊肿的新发现。

本研究的目的是了解奶牛卵泡囊肿的相关因素。该研究包括体内和体外方法。在体内部分，对健康(H)和卵泡囊肿（FC）奶牛的血清进行激素评估（Kisspeptin-10 [Kp-10]、促性腺激素抑制激素[GnIH]、促黄体生成素[LH]、雌激素[E2]和皮质醇）。体外部分主要研究GPR54 （Kp-10受体）和GPR147 （GnIH受体）在囊泡和排卵前卵泡上的分布。与h组相比，FC组血清Kp-10、GnIH、LH、E2和皮质醇浓度显著升高。基因表达分析显示，与排卵前卵泡相比，FC组GPR54 mRNA水平降低，未检测到GPR147受体的表达。与排卵前卵泡相比，卵巢FC中GPR54的含量较低，这可以通过卵巢FC奶牛血清中Kp-10浓度升高诱导的受体下调来确定。下丘脑-垂体-卵巢轴的内分泌失衡是FC的特征，可能反映了Kp-10和GnIH分泌模式的改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.