Regenerative Biomaterials最新文献

{"title":"Sr-doped surfaces with 2D black phosphorus nanosheets for enhanced photothermal antibacterial activity and zirconia implant osseointegration.","authors":"Huan Cheng, Jiaquan Chen, Yan Wang, Yinyan Zhang, Tianyun Qin, Haobo Sun, Wen Si, Ningyao Sun, Yingyue Sun, Lifeng Xiong, Zhennan Deng, Lei Lu, Peng Gao, Jinsong Liu","doi":"10.1093/rb/rbaf033","DOIUrl":"10.1093/rb/rbaf033","url":null,"abstract":"<p><p>Zirconia (ZrO₂) has emerged as a preferred material for dental implants due to its excellent chemical inertness, absence of metal allergies and esthetic appeal. However, its limited bioactivity regarding infection resistance and early osseointegration hinders its implantation success rate compared to titanium implants. Herein, we developed a PDPA@Sr/BP coating for ZrO₂ implants to address these limitations. First, inspired by the adhesive properties of mussel foot proteins, a PDPA@Sr coating enriched with positively charged amine groups and strontium (Sr) ions was applied to the ZrO₂ surface. This coating stably anchored black phosphorus (BP) to the implant, effectively regulating its degradation rate and ensuring long-lasting antibacterial properties. Under near-infrared (NIR) light irradiation, BP generated localized heat, efficiently killing bacteria. Simultaneously, the release of Sr and phosphate ions from the PDPA@Sr/BP coating promoted bone formation and enhanced osseointegration. This study systematically evaluated the antibacterial effects and osseointegration-promoting properties of the PDPA@Sr/BP coating through both <i>in vitro</i> and <i>in vivo</i> experiments. The results demonstrated that compared to untreated ZrO₂ surfaces, the coating significantly enhances the implant's antibacterial properties and accelerates its surface osseointegration. This study proposes an innovative strategy to improve the clinical performance of ZrO₂ implants, demonstrating substantial potential for clinical translation.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf033"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels.","authors":"Jun Liu, Qian Liu, Minmei Guo, Chengyu Jiang, Jianyang Chen, Ting Wang, Tzu-Cheng Sung, Shih-Jie Chou, Shih-Hwa Chiou, Guoping Fan, Akon Higuchi","doi":"10.1093/rb/rbaf035","DOIUrl":"10.1093/rb/rbaf035","url":null,"abstract":"<p><p>A variety of novel peptide-grafted hydrogels, of which peptides were derived from vitronectin (PQVTRGDVFTMP) or the laminin β4 chain (PMQKMRGDVFSP), were prepared in this study. The peptide-grafted hydrogels promoted the adhesion, proliferation and colony formation of hiPSCs and maintained their pluripotency up to passage 5 under xeno-free conditions. We successfully generated RPE cells from hiPSCs using one of the most suitable xeno-free peptide-grafted hydrogels, KVN2CK (KGCGGKGG-PQVTRGDVFTMP), which was derived from vitronectin, and confirmed the effect of these hiPSC-derived RPE cells in a rat retinal degeneration model (Royal College of Surgeons (RCS) rats) via subretinal transplantation, when we investigated functional improvements in vision in RCS rats after the transplantation of hiPSC-derived RPE cells. Our novel peptide-grafted hydrogels provided a safe and robust platform for generating single-layer hiPSC-derived RPE cells under xeno-free conditions, which indicates the potential of these hydrogels for stem cell therapy for retinal degenerative diseases in the future.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf035"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnesium as an emerging bioactive material for orthopedic applications: bedside needs lead the way from innovation to clinical translation.","authors":"Ningze Zhang, Qida Zhang, Hongwei Shao, Zhengming Shan, Jiankun Xu, Wenxue Tong, Ronald Man Yeung Wong, Ling Qin","doi":"10.1093/rb/rbaf032","DOIUrl":"10.1093/rb/rbaf032","url":null,"abstract":"<p><p>With the rapid increase in population aging, the number of surgical operations in orthopedics is expected to increase. The gap between the materials applied in clinical orthopedics and materials in discovery and research is obvious due to regulatory requirements for biosafety and treatment efficacy. For the bedside needs, it is important to overcome hurdles by achieving impactful innovation and clinical translation of orthopedic materials. Magnesium (Mg), as an emerging bioactive material, is one of the vital components of the human body and mainly stored in the musculoskeletal system as either a matrix component or an intracellular element for the homeostasis of various physiological functions. However, the degradation and biomechanical performance limit the applications of Mg. This review aims to explore the current challenges and future directions of Mg for clinical translation and provide an update on biomaterials used in orthopedics, factors driving orthopedic innovation, physiology of magnesium ions (Mg²⁺) and its potential clinical applications. To achieve orthopedic application, modification of the performance of Mg as implantable metals and function of the degradation products of Mg <i>in vivo</i> are described. For the clinical needs of treating the steroid-associated osteonecrosis (SAON), Mg screws and Mg-based composite porous scaffolds (Mg/PLGA/TCP: magnesium/poly(lactic-co-glycolic acid) (PLGA)/tricalcium phosphate (TCP)) have been developed, but the challenges of Mg-based implants in load-bearing skeletal sites still exist. To utilize the beneficial biological effects of Mg degradation and overcome the weakness in mechanical stability for fracture fixation, the concept of developing Mg/titanium (Ti) hybrid orthopedic implants is reported, where the Ti component provides effective mechanical support while the inclusion of Mg component potentially optimizes the biomechanical properties of Ti component and facilitate bone healing. This review provides a reference frame for the translation of novel materials and promotes the development of innovative orthopedic biomaterials for clinical applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf032"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on osteoclast regulation by biodegradable magnesium and its mechanism.","authors":"Wangwei Zhu, Weidan Wang, Xing Yang, Chunxiao Ran, Tianwei Zhang, Shibo Huang, Jiahui Yang, Fuyang Wang, Huiya Wang, Peng Wan, Fengyuan Piao, Faqiang Lu, Shengbo Shi, Ye Li, Xiuzhi Zhang, Dewei Zhao","doi":"10.1093/rb/rbaf026","DOIUrl":"10.1093/rb/rbaf026","url":null,"abstract":"<p><p>Continuous advancements in medical technology and biomaterials have underscored the significant advantages of biodegradable implant materials for bone repair and remodelling over traditional inert metallic implants. Notably, biodegradable magnesium-based materials have gained much attention because of their optimal corrosion rates. Importantly, extensive clinical experience has resulted in the use of biodegradable magnesium-based orthopaedic implants. Both preclinical and clinical studies have consistently demonstrated that Mg has an excellent ability to promote bone tissue formation, a process that is closely associated with the release of Mg²⁺ and other degradation byproducts. Bone metabolism depends on a dynamic balance of bone formation and bone resorption. Mg²⁺ has been shown to increase osteoblast (OB) activity while suppressing osteoclast (OC) formation, thus playing a crucial role in bone remodelling and regeneration. In terms of osteolysis inhibition, Mg²⁺ plays a multifaceted role. First, Mg²⁺ inhibits OC formation by modulating the activity of mature OCs, their migratory behaviour and the activity of precursor cells. Second, Mg²⁺ influences OC production by regulating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor kappa-Β ligand (RANKL) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Additionally, Mg²⁺ impacts bone resorption by altering the immune microenvironment and the levels of hormones and peptides within the body. Furthermore, the alkaline environment generated around the biodegradable magnesium implant and its degradation products (e.g. H₂) also significantly inhibit OC formation. Recent research on magnesium-based implants has focused predominantly on their osteogenic properties, with few systematic reviews addressing the mechanisms through which biodegradable magnesium alloys suppress osteoclastic activity. This article summarizes the latest clinical research progress concerning biodegradable magnesium implant materials and their significant regulatory effects and discusses recent advances in the understanding of the regulatory mechanisms of action Mg-based biomaterials on OCs, with the aim of providing a more theoretical basis for the clinical application of biodegradable magnesium-based implants.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf026"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-inspired lotus-fiber and mussel-based multifunctional hydrogels for wound healing: super-stretchability, self-healing, adhesion and antibacterial properties.","authors":"Xiaoling Yang, Chenchen Li, Bo Li, Yuanyuan Zhang, Jinping Li, Na Liu, Xin Nie, Dawei Zhang, Ming Zhou, Xiaoling Liao","doi":"10.1093/rb/rbaf031","DOIUrl":"10.1093/rb/rbaf031","url":null,"abstract":"<p><p>Hydrogel-based wound dressings, which facilitate rapid wound closure and healing, are essential for effective wound management. However, the development of an ideal hydrogel that possesses excellent mechanical properties, effective self-healing capabilities, tissue adherence and antimicrobial characteristics for wound dressing presents a significant challenge in clinical settings. Inspired by lotus-fiber and mussel, we synthesized a novel multifunctional hydrogel composed of bacterial cellulose-reinforced dopamine-grafted oxidized hyaluronic acid/polyacrylamide (OHA-DA/PAM/BC). This was achieved through a one-pot reaction that employed free radical polymerization of acrylamide, dynamic Schiff bonding and intermolecular hydrogen bonding. Compared with the pure PAM hydrogels, which exhibited an elongation at break of 4022% and a maximum tensile strength of 26.42 kPa, the OHA-DA/PAM hydrogel demonstrated significantly enhanced stretchability at 9949% and an increased tensile strength of 34.73 kPa when 0.3% OHA-DA was incorporated during hydrogel formulation. Notably, the addition of 0.8% BC significantly enhanced the tensile strength to 57.04 kPa and super-stretchability to 10679%. The OHA-DA/PAM/BC hydrogel also exhibited remarkable self-healing capabilities, achieving a mechanical recovery of 84.74% within 12 h. Additionally, its adhesive and injectable properties are advantageous for dynamic wound repair. Furthermore, the OHA-DA/PAM/BC hydrogel exhibited minimal hemolytic activity and potent intrinsic antibacterial properties against both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. In a mouse model of wound healing, this hydrogel reduced the healing duration to 14 days while enhancing the regeneration of both skin structure and function. Histological analyses further revealed that the hydrogel significantly promoted the development of well-organized granulation tissue, angiogenic tissue and collagen accumulation in the wound region. This study successfully developed an OHA-DA/PAM/BC multifunctional hydrogel characterized by exceptional stretchability, self-healing, adhesiveness, injectability and antibacterial activity, demonstrating a significant impact on wound healing <i>in vivo</i>. These findings indicated that the OHA-DA/PAM/BC hydrogel holds substantial potential as wound dressings for future clinical applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf031"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural product-based nano-antioxidant for the treatment of acute pancreatitis.","authors":"Xiaoluan Lu, Ze Gao, Yanling Yu, Lang Zhang, Jing Huang, Xiaoming Zhang, Pei Jing, Shiyong Zhang, Mei Zeng","doi":"10.1093/rb/rbaf012","DOIUrl":"10.1093/rb/rbaf012","url":null,"abstract":"<p><p>Acute pancreatitis (AP) is a potentially highly fatal inflammatory disease characterized by the generation of high level of reactive oxygen species (ROS) of mass recruited inflammatory macrophages in pancreatic tissue. Many natural product antioxidants have been explored to treat AP due to their superiority in biosafety while the therapeutic application is restricted by their low ROS elimination as well as the rapid metabolism caused by small molecular weight and fast absorption. Herein, a new natural product-based nano-antioxidant (FA@zein-CS) that can overcome these problems was developed for the treatment of AP by encapsulating ferulic acid (FA) into the zein based nanoparticles and then hybridizing of chondroitin sulfate (CS). The FA@zein-CS would not only efficiently target to the inflamed pancreatic tissue by the specific binding of CS to CD44, but also effectively initiate the release of FA and zein degradation product in response to intracellular pH/GSH/ROS to achieve synergistic antioxidant effect. In addition, thanks to the fact that all components were derived from natural products, the FA@zein-CS held the excellent biocompatibility. <i>In vivo</i> results disclosed that the FA@zein-CS significantly reduced pancreatic structural damage and restored the pancreatic function with serum amylase and lipase reduced by 61.8% and 82.8%, respectively. This natural product-based nano-antioxidant holds great clinic potential for AP.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf012"},"PeriodicalIF":5.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vivo</i> dynamic visualization and evaluation of collagen degradation utilizing NIR-II fluorescence imaging in mice models.","authors":"Shunyao Li, Kai Xu, Huaixuan Sheng, Huizhu Li, Xiao Zhang, Chengxuan Yu, Haichen Hu, Xiner Du, Yunxia Li, Yu Dong, Jun Chen, Sijia Feng","doi":"10.1093/rb/rbaf025","DOIUrl":"10.1093/rb/rbaf025","url":null,"abstract":"<p><p>Collagen-based biomaterials are gaining prominence in tissue engineering, attributed to their remarkable biocompatibility, inherent biodegradability, and unparalleled capacity to facilitate tissue repair and regeneration. However, the ability to dynamically visualize and quantitatively assess collagen degradation <i>in vivo</i> remains a critical challenge, hindering the development of optimized biomaterials for clinical applications. To address this, a novel approach was developed to monitor the injury microenvironment by conjugating second near-infrared quantum dots with solid collagen. This live imaging system offered high-resolution, real-time tracking of collagen degradation both <i>in vitro</i> and <i>in vivo</i>, enabling a deeper understanding of the degradation behavior under various conditions. This system was applied to mouse models with different cartilage defects, including critical-sized defect (CSD), minor defect (Minor) and sham surgery (Sham) groups for a 28-day <i>in vivo</i> monitoring. Among them, the CSD group exhibited the fastest and most stable collagen degradation, indicating that the degradation rate was closely linked to the severity of the injury. Transcriptomic analysis further identified key signaling pathways that might drive rapid collagen degradation by promoting collagenase activity and tissue remodeling in cartilage defect conditions. In summary, our study provided valuable insights into the mechanisms of collagen degradation under different injury conditions, contributing to innovative strategies for designing collagen-related biomaterials in the future.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf025"},"PeriodicalIF":5.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials-based approaches to mandibular tissue engineering: where we were, where we are, where we are going.","authors":"Maeve M Kennedy, Antonios G Mikos","doi":"10.1093/rb/rbaf024","DOIUrl":"https://doi.org/10.1093/rb/rbaf024","url":null,"abstract":"<p><p>The mandible is the largest craniofacial bone and plays a crucial role in speech, mastication, swallowing, and facial aesthetics. The form or function of the mandible can be altered by defects as a result of tumors, trauma, infection, and congenital conditions. This paper covers the evolution of biomaterials-based approaches to the reconstruction of critical size mandibular defects. Historically the gold standard for critical size mandibular defect repair has been autologous fibula grafts. The emergence of the field of tissue engineering has led to the current research on biomaterial scaffolds, cells, and biological factors to design highly tunable, bio-inspired, tissue regenerative implants. Scaffold materials can be synthetic or natural and can be fabricated using a variety of additive manufacturing techniques. Mesenchymal stem cells, bone morphogenetic proteins, and transforming growth factor-β are frequently added to scaffolds. While great progress has been made, there are still barriers to translating this research to patients, ranging from insufficient bone regeneration in animal studies to the feasibility of establishing a good manufacturing practice. To address these challenges, the future of mandibular tissue engineering will look toward improving implant vascularization and innervation, personalizing implant shape and biology, and enhancing spatiotemporal control of drug release. With these goals in mind, researchers will ultimately develop biomaterials that can regenerate bone that is structurally and biologically identical to native mandibular tissue, improving both function and quality of life for patients.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf024"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-yield BMSC-derived exosomes by the 3D culture system to enhance the skin wound repair.","authors":"Jie Wu, Siqi Li, Hao Wang, Yuanbo Qi, Sheng Tao, Peifu Tang, Daohong Liu","doi":"10.1093/rb/rbaf022","DOIUrl":"https://doi.org/10.1093/rb/rbaf022","url":null,"abstract":"<p><p>Wound defects pose a substantial challenge in clinical practice, often resulting in prolonged healing times and an elevated risk of infection. Insufficient vascularization is a critical factor that adversely affects wound healing. Exosomes obtained from bone mesenchymal stem cells (BMSC-exos) have demonstrated significant promise in accelerating tissue repair by promoting angiogenesis. However, their limited yield and suboptimal biological functions impede widespread clinical application in enhancing wound healing. Prior research has indicated that 3D cultures can boost exosome secretion when compared to conventional 2D cultures. However, the currently prevalent 3D culture methods often necessitate expensive equipment or cumbersome procedures. This study investigates a cost-effective and user-friendly 3D culture system developed using gelatin methacrylate (GelMA). Our findings indicate that a 5% concentration of GelMA provides an optimal environment for the 3D culture of BMSCs. Furthermore, we observed that 3D culture significantly delays the senescence of BMSCs, thereby creating favorable conditions for the sustained production of exosomes. Additionally, 3D cultivation has the potential to boost exosome secretion and enhance their angiogenic capabilities. <i>In vivo</i> experiments further confirmed that BMSC-exos from a 3D environment exhibit enhanced capabilities to promote wound healing. These results suggest that GelMA-based 3D cultures offer a novel strategy for both industrial production and clinical application of exosomes.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf022"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimensional effects of surface morphology and trapped air on mammalian cell adhesion to special wetting surfaces.","authors":"Zhiwei Chen, Yun Yang, Shaohua Xu, Zhenyu Shen, Yijian Tang, Yisheng Lin, Qiaoling Huang","doi":"10.1093/rb/rbaf021","DOIUrl":"https://doi.org/10.1093/rb/rbaf021","url":null,"abstract":"<p><p>Materials with special wettability have broad biomedical applications, including the control of mammalian cell adhesion and inhibiting biofilm formation. However, limited understanding of mammalian cellular responses to superhydrophobic materials with trapped air restricts their clinical applications. In this study, we fabricated materials with varied nanostructures and wettability, and systematically compared short-term mammalian cellular responses in the presence and absence of trapped air. Our results show that small nanostructures generate small, often invisible air bubbles at the solid-liquid interface when in contact with mammalian cell suspensions. In the presence of these small bubbles, the number of adhered cells was comparable to both the same sample without trapped air and its hydrophilic counterpart, contradicting the intuitive expectations that trapped air would reduce cell adhesion. In contrast, larger nanostructures resulted in visible, hundred-micron-sized air bubbles, which significantly inhibited cell adhesion. This effect was evident when comparing the same superhydrophobic sample with and without trapped air, as well as against hydrophilic counterparts with the same morphology. Further tracking of large air bubbles on the hydrophobic materials revealed that no cells adhered to the areas occupied by hundred-micron-sized air bubbles, while more cells accumulated at the solid-liquid-gas triple line. Hence, this work deepens the understanding of cellular responses to superhydrophobic materials, revealing that material structure size influences the size of trapped air and subsequently dominates cell adhesion.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf021"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12017620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}