Regenerative Biomaterials最新文献

{"title":"Advanced materials and devices strategies of mechanical stimulation for tissue regeneration.","authors":"Mengting Huan, Huihui An, Xing Sheng, Lan Yin, Liu Wang","doi":"10.1093/rb/rbag054","DOIUrl":"10.1093/rb/rbag054","url":null,"abstract":"<p><p>Mechanical stimulation as a form of physical therapy plays a crucial role in regulating cellular behavior and promoting tissue regeneration. It can influence cell proliferation, differentiation, migration and extracellular matrix deposition, thereby providing a powerful biophysical cue in regenerative medicine. The implementation of precisely controllable mechanical stimulation relies on efficient and reliable tool platforms that can deliver defined magnitudes, frequencies, directions and temporal patterns of force, while maintaining high reproducibility and safety in biological environments. For mechanical stimulation of cells and injured tissues, a variety of materials as well as devices with distinct force outputs, such as stretchable elastic substrates, microfluidic shear systems and mechano-active scaffolds have been designed to meet diverse experimental and therapeutic requirements. Moreover, functional materials integrated with magnetic, acoustic and optical modalities are also been developed to establish remote mechanical stimulation systems, enabling spatiotemporally programmable interventions even in deep or delicate tissues. This review summarizes recent advances of the <i>in vitro</i> and <i>in vivo</i> strategies that leverage mechanical stimulation in regenerative medicine, along with its regulatory effects on regenerative processes in neural, skeletal, muscular and other tissues. Finally, the major challenges and prospects regarding materials and devices for mechanical stimulation in tissue therapy are discussed.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag054"},"PeriodicalIF":8.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acellular nerve perineurium repairs peripheral nerve injuries in a rat model via ECM-mediated barrier and optimization of the regenerative microenvironment.","authors":"Fulin He, Zhenpeng Li, Fawei Liao, Shuihuan Wang, Xijun Huang, Jiahui Sun, Aijun Huang, Siyu LinFang, Tao Lin, Zilong Rao, Xiaolin Liu, Shuo Tang, Qingtang Zhu, Canbin Zheng, Shuai Qiu","doi":"10.1093/rb/rbag047","DOIUrl":"https://doi.org/10.1093/rb/rbag047","url":null,"abstract":"<p><p>Building upon the clinically established platform of acellular nerve allografts (ANAs), we developed an advanced derivative: acellular nerve perineurium (ANP) grafts. These grafts are characterized by their preservation of the native perineurial barrier architecture and a unique extracellular matrix (ECM) composition, distinct from the endoneurial basement membrane. This distinctive ECM profile endows ANPs with significantly enhanced barrier integrity and robust neuroprotective properties. <i>In vitro</i> evaluations confirmed that ANP provides a highly favorable substrate, effectively supporting the adhesion and active proliferation of dorsal root ganglion neurons. In a rat model of sciatic nerve transection, ANP grafts demonstrated remarkable therapeutic efficacy. They markedly inhibited the deposition of chondroitin sulfate proteoglycans (CSPGs) at the repair site, thereby preventing traumatic neuroma formation. Furthermore, ANP treatment resulted in a doubling of regenerated axon density and a significant increase in target muscle action potential amplitude. Behavioral recovery in ANP-treated animals approached the functional levels observed in crush-injury controls. Multiomics analyses provided mechanistic insight, revealing that ANP-mediated repair activates multiple pro-regenerative signaling pathways. These collective findings position ANP grafts as a highly promising and clinically translatable biomaterial strategy for improving functional outcomes in peripheral nerve repair.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag047"},"PeriodicalIF":8.1,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimuli-responsive hydrogels for radiation-induced skin injury: from passive barriers to autonomous drug delivery systems.","authors":"Kai Zhang, Chulan Xiao, Yuanyuan Wang, Caitong Zhao, Zhenghang Dong, Zhihui Li, Xinmao Song, Chuanglong He, Yi Li","doi":"10.1093/rb/rbag056","DOIUrl":"10.1093/rb/rbag056","url":null,"abstract":"<p><p>Radiation-induced skin injury (RISI) affects over 95% of radiotherapy patients. Current clinical management remains confined to passive supportive care, lacking mechanistic precision for RISI's unique pathophysiology. This review adopts a function-centric perspective, classifying hydrogel systems across three generations: first-generation passive moisture barriers; second-generation bioactive platforms incorporating antioxidants, growth factors, stem cells and exosomes; and third-generation stimuli-responsive systems integrating autonomous drug release, self-healing capabilities and biosensor monitoring. We establish quantitative design thresholds by correlating RISI microenvironment parameters (pH 6.5-7.0, ROS 100-500 μM, MMP-9 elevation 5-10×) with responsive polymer specifications. Single-cell transcriptomic analysis has identified pro-inflammatory IL-17⁺ secretory fibroblasts and dysfunctional lymphatic endothelial cells as key dysregulated populations, thereby defining precise cellular targets amenable to hydrogel-based intervention. However, randomized trials demonstrate that certain hydrogel formulations unexpectedly prolonged healing, underscoring the need for design strategies based on quantitative pathophysiological insights rather than passive empiricism. We systematically examine enabling technologies-AI-guided materials optimization, 3D bioprinting and wearable biosensor integration-while addressing translational barriers including regulatory complexity, manufacturing scalability and standardized preclinical models. This framework provides actionable design principles to accelerate clinical deployment of next-generation hydrogels for millions of cancer survivors.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag056"},"PeriodicalIF":8.1,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional-printed silk fibroin scaffolds loaded with adipose-derived stem cells prevent post endoscopic submucosal dissection esophageal stricture in a porcine model.","authors":"Shujun Ye, Daxu Zhang, Xiaonan Shi, Zhanbo Wang, Jingjing Hu, Shuo Zhao, Weilong Li, Jingyi Wang, Weiping Guan, Lianjun Ma, Li Yan","doi":"10.1093/rb/rbag057","DOIUrl":"https://doi.org/10.1093/rb/rbag057","url":null,"abstract":"<p><p>Endoscopic submucosal dissection (ESD) is the preferred treatment for early esophageal cancer. However, extensive mucosal dissection frequently results in esophageal stricture. This study aimed to evaluate the efficacy of Three-dimensional (3D)-printed silk fibroin scaffolds (SFS) loaded with adipose-derived stem cells (ADSCs) in preventing post-ESD esophageal stricture, and to explore the underlying mechanisms. A near-circumferential ESD model was established in pigs, three groups were set: Control, SFS and ADSCs-SFS. The dynamic change of postoperative esophageal healing and stricture formation were monitored endoscopically. On postoperative Day 28, esophageal specimens were collected to measure mucosal contraction rate, followed by histological evaluation of inflammation and fibrosis, as well as mRNA transcriptome sequencing to analyze gene expression and the change of the enriched signaling pathways. On Day 28, the ADSCs-SFS group showed significantly less weight loss and a markedly reduced mucosal contraction rate. Histological examination revealed more complete mucosal regeneration and significantly reduced collagen deposition in the ADSCs-SFS group. Molecular analyses indicated significant downregulation of inflammatory and fibrotic markers in the ADSCs-SFS group. Transcriptome analysis suggested that ADSCs-loaded SFS effectively alleviates esophageal stricture following ESD in pigs, likely through suppression of the PI3K/AKT signaling pathway and reduction of inflammation and fibrosis.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag057"},"PeriodicalIF":8.1,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterial regulation of excessive inflammation restores osteoimmune homeostasis in diabetic bone regeneration.","authors":"Qiyue Zhou, Yu Zhang, Zhuo Dai, Qiang Li, Weijun Xiu, Haoxin Lv, Yongbin Mou, Heng Dong","doi":"10.1093/rb/rbag055","DOIUrl":"https://doi.org/10.1093/rb/rbag055","url":null,"abstract":"<p><p>Diabetes mellitus markedly increases the incidence of fractures, implant failure and nonunion, primarily because chronic low-grade inflammation and a disrupted bone microenvironment impair regeneration. Under physiological conditions, coordinated interactions among immune, stromal, vascular and neural cells ensure timely initiation and resolution of inflammation, thereby maintaining osteoimmune homeostasis and supporting bone repair. In diabetes, hyperglycemia-induced oxidative stress, advanced glycation end products, impaired vascularization and 'inflammatory memory' prolong and intensify inflammatory responses. This excessive and unresolved inflammation disturbs immune-bone crosstalk, alters macrophage and T-cell phenotypes and uncouples osteogenesis and angiogenesis, ultimately hindering bone regeneration. This review summarizes the cellular and molecular basis of osteoimmune homeostasis and outlines how diabetes disrupts this regulatory network at systemic and local levels. We further highlight biomaterial strategies designed to modulate excessive inflammation and restore osteoimmune balance in diabetic bone regeneration, including localized delivery systems, cell-derived and extracellular vesicle-based agents, nanozyme-mediated microenvironmental regulation and immune-instructive physicochemical biomaterials. Finally, we discuss the critical hurdles of clinical translation (e.g. standardized scalable fabrication and long-term biosafety) and highlight multifactor integrated, logic-gated designs and systemic diabetes management strategies for advancing next-generation biomaterials for diabetic bone regeneration.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag055"},"PeriodicalIF":8.1,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silk-coated PLGA non-spherical microparticles for simvastatin delivery in rat maxillofacial bone regeneration.","authors":"Junjiang Zhang, Yuqi Chang, Yiwen Zhou, Yang Zhou, Jiayin Li, Feng Wang, Zhimin Zhou, Lei Sui, Yanjing Li","doi":"10.1093/rb/rbag053","DOIUrl":"https://doi.org/10.1093/rb/rbag053","url":null,"abstract":"<p><p>Maxillofacial bone defects present a major challenge in clinical practice. Bone grafting and bone tissue engineering are limited by various side effects and potential risks, while local drug delivery is regarded as a convenient and efficient strategy for bone repair and regeneration. However, for bone tissue regeneration, a drug delivery system should not only release osteogenic drugs but also possess an appropriate morphology to provide structural support for cell proliferation and differentiation. Polymeric non-spherical counterparts may offer new osteogenic properties through altered cellular interactions, biodistribution, and immune responses, compared with spherical drug carriers. Poly(lactic-co-glycolic acid) (PLGA) has been widely used to prepare various drug carriers due to its excellent biocompatibility and controllable biodegradation. Shape regulation, surface modification and drug incorporation are effective approaches to impart distinct functions to PLGA particles for various biomedical applications. However, PLGA has inherent limitations, including poor hydrophilicity, acidic degradation products and a lack of functional ligands for cell attachment, which hinder its effectiveness in bone regeneration. To solve these problems, natural proteins were utilized to improve the bone/cartilage regeneration of polymeric particles/scaffolds through surface modification or physical complexation due to their excellent biocompatibility and biodegradability. In this study, we successfully developed simvastatin (SIM)-loaded disc-shaped PLGA microparticles with coarse surface (dPLGA) using W₁/O/W₂ followed by silk fibroin (SF) coating for hydrophilicity and biocompatibility improvement. <i>In vitro</i> and <i>in vivo</i> experiments revealed that SF-modified SIM-loaded dPLGA (SF-SIM@dPLGA) significantly promoted the osteogenic differentiation of stem cells and bone regeneration in cranial bone defects, indicating the superior osteoconductive and osteoinductive advantages arising from the physical morphology of particles and chemical effects. Additionally, SF-SIM@dPLGA demonstrated improved tissue adhesion and hemostatic ability. This novel PLGA non-spherical particle-based local delivery system provides a feasible strategy for practical maxillofacial bone repair due to the simple preparation procedures and enhanced bone regeneration performance.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag053"},"PeriodicalIF":8.1,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13082902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond labeling: differential Ac₄ManNAz dosing as a tool for functional manipulation of mesenchymal stem cells.","authors":"Xueying Zhao, Suqing Li, Xingyu Jiang, Yuyang Ma, Kewei Fan, Luzhong Zhang, Xin Liu, Yumin Yang","doi":"10.1093/rb/rbag044","DOIUrl":"https://doi.org/10.1093/rb/rbag044","url":null,"abstract":"<p><p>Bioorthogonal chemistry technology serves as a powerful tool for mesenchymal stem cell (MSC) transplantation by utilizing metabolic engineering for direct cell modification either <i>in vivo</i> or <i>in vitro</i>. However, the unclear effects of varying concentrations of non-natural metabolic monosaccharides on labeling efficiency, functional impact and safety pose significant challenges for clinical translation. Herein, we screened the concentration-dependent effects of the metabolic labeling agent Ac₄ManNAz (10-100 µM) on the functionality of MSC. Visualizing azido groups on the cell surface through bioorthogonal reactions confirmed that labeling efficiency positively correlated with the concentration of Ac₄ManNAz. Interestingly, treatment of MSC with 50-µM Ac₄ManNAz enhances their immune regulatory function. Mechanistically, 50-µM Ac₄ManNAz remodels the extracellular matrix of MSC by downregulating decorin (DCN) expression to alleviate TGF-<b>β</b> inhibition, simultaneously upregulating serglycin (SRGN) expression to activate and stabilize TGF-<b>β</b>. This dual action results in increased secretion of TGF-<b>β</b>, thereby augmenting the immune regulatory capacity of MSC. Nevertheless, at the level of cellular motility, treatment with 50-µM Ac₄ManNAz significantly impairs the migratory capacity of MSC. Overall, this study clearly defined the concentration effect of non-natural monosaccharides on MSC biological functionality for clinical applications of this technology.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag044"},"PeriodicalIF":8.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13138843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria-targeted nanotechnology in cardiovascular diseases: a review of recent advances.","authors":"Sijia Sun, Manxiang Wu, Pengli Zhang, Linglin Sun, Wenjing Zhong, Sisi Zheng, Yanjin Li, Jianbin Li, Qiang Li","doi":"10.1093/rb/rbag040","DOIUrl":"https://doi.org/10.1093/rb/rbag040","url":null,"abstract":"<p><p>Cardiovascular diseases (CVDs) remain the leading cause of global mortality, with mitochondrial dysfunction serving as a central pathological hub in conditions such as atherosclerosis, myocardial ischemia-reperfusion injury and heart failure. Current mitochondrial-regulating drugs are severely limited by low bioavailability, short duration of action, poor targeting specificity and off-target effects, highlighting an urgent need for precise delivery systems. Nanocarriers, with tunable physicochemical properties and surface functionalization potential, enable hierarchical targeting of diseased cardiac tissues and mitochondria, offering a novel solution to overcome these limitations. Preclinical models have shown promising efficacy, particularly in alleviating oxidative stress damage in ischemic cardiomyopathy, improving energy metabolism in heart failure and promoting tissue repair. These encouraging results have sparked growing interest in the application of nanomaterials for mitochondrial-targeted diagnosis and treatment of CVDs. This review first outlines the role of mitochondrial dysfunction in CVD pathogenesis, covering impaired oxidative phosphorylation, excessive reactive oxygen species production, disrupted mitochondrial dynamics and defective mitophagy. It, then, focuses on the design strategies of nanotherapeutics based on a hierarchical targeting concept, encompassing the selection of biocompatible carriers, optimization of size and morphology, tissue or cell-specific targeting modifications, mitochondrial ligand modifications, as well as the loading and therapeutic mechanisms of various therapeutic agents. Furthermore, it provides an in-depth analysis of key physiological barriers such as hemodynamic shear stress, endothelial barrier and extracellular matrix hindrance, along with intracellular trafficking challenges including lysosomal escape and immune clearance, which all impact delivery efficiency. This review aims to offer insights to advance the rational development and clinical translation of mitochondria-targeted nanomedicines for CVDs.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag040"},"PeriodicalIF":8.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13095658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifunctional EGCG/Si nanohybrid-coated 3D-printed porous scaffold for bone defect repair.","authors":"Shiqi Xiao, Lin Qi, Jiacheng Wei, Huan Liu, Sheng Ding, Ju Chen, Jin Yang, Hua Lin, Dianxiang Lu","doi":"10.1093/rb/rbag035","DOIUrl":"https://doi.org/10.1093/rb/rbag035","url":null,"abstract":"<p><p>Besides enhancing osteogenesis and angiogenesis, designing multifunctional scaffolds with immunomodulatory capabilities offers a promising strategy for managing bone defects. Herein, a 3D-printed scaffold modified with inorganic silicon (Si) ions and the polyphenol epigallocatechin gallate (EGCG) was constructed as an immunomodulatory biocomposite with coupled angiogenic and osteogenic activity to enhance bone regeneration. Through phenol-amine chemistry and electrostatic layer-by-layer deposition, a nanohybrid EGCG/Si coating was fabricated on the poly(lactic-co-glycolic acid) scaffold surface. Our <i>in vitro</i> studies demonstrated that the released Si ions, in combination with the surface topological morphology, promoted osteogenic differentiation of bone mesenchymal stem cells and enhanced the ability of human umbilical endothelial cells to form patterned vascular networks. The EGCG-coated scaffold not only provides effective protection against reactive oxygen species-mediated cellular damage but also actively modulates the immune microenvironment by inducing macrophage polarization toward a reparative phenotype, enhancing the expression of anti-inflammatory factors and inhibiting pro-inflammatory gene expression. <i>In vivo</i> experiments further revealed that the coated scaffolds exhibited significant potential in enhancing new bone formation within rat femoral defects. Transcriptomic profiling indicated that Si ions, in conjunction with surface topography, cooperatively triggered multiple signaling pathways involved in cell adhesion, proliferation and differentiation. Overall, this EGCG/Si nanocomposite coating approach presents a novel avenue for developing multifunctional scaffolds in bone tissue engineering.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag035"},"PeriodicalIF":8.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13070653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional recovery after spinal cord injury through neuroprotection by lipoic acid-loaded hollow mesoporous Prussian blue nanozymes.","authors":"Qiannan Zhao, Yuanlong Li, Jiaqi Zhang, Kai Gao, Lin Shi, Ensi Liu, Wenjuan Yang, Tingting Zhang, Xifan Mei, Zhaoliang Shen","doi":"10.1093/rb/rbag039","DOIUrl":"https://doi.org/10.1093/rb/rbag039","url":null,"abstract":"<p><p>The key obstacle to functional recovery after spinal cord injury (SCI) is the imbalance of the oxidative stress microenvironment in the injured area. Traditional drug therapies have limitations in regulating this environment and eliminating the excessive accumulation of reactive oxygen species (ROS) is crucial. In this study, an environmentally friendly and economical recombinant nanoenzyme (LA-HMPB) was successfully constructed, which achieves delivery to the SCI injury site and enhances the therapeutic capacity of lipoic acid (LA). This nanoenzyme alleviates oxidative stress through the Keap1/Nrf2 pathway, thereby promoting functional recovery after SCI. The research found that HMPB not only serves as a carrier but also enhances the antioxidant stress capacity of LA. After administration, LA-HMPB can distribute to the SCI site and exert its effects. It has been confirmed that this formulation reduces oxidative stress levels by regulating the Keap1/Nrf2 pathway, thereby promoting functional recovery. This natural nano-drug delivery platform strategy opens up broad prospects for the clinical treatment of SCI and provides a useful reference for the research on antioxidant therapy for other neurological diseases.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag039"},"PeriodicalIF":8.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13070658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147676328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}