Regenerative Biomaterials最新文献

{"title":"Research progress on osteoclast regulation by biodegradable magnesium and its mechanism.","authors":"Wangwei Zhu, Weidan Wang, Xing Yang, Chunxiao Ran, Tianwei Zhang, Shibo Huang, Jiahui Yang, Fuyang Wang, Huiya Wang, Peng Wan, Fengyuan Piao, Faqiang Lu, Shengbo Shi, Ye Li, Xiuzhi Zhang, Dewei Zhao","doi":"10.1093/rb/rbaf026","DOIUrl":"10.1093/rb/rbaf026","url":null,"abstract":"<p><p>Continuous advancements in medical technology and biomaterials have underscored the significant advantages of biodegradable implant materials for bone repair and remodelling over traditional inert metallic implants. Notably, biodegradable magnesium-based materials have gained much attention because of their optimal corrosion rates. Importantly, extensive clinical experience has resulted in the use of biodegradable magnesium-based orthopaedic implants. Both preclinical and clinical studies have consistently demonstrated that Mg has an excellent ability to promote bone tissue formation, a process that is closely associated with the release of Mg²⁺ and other degradation byproducts. Bone metabolism depends on a dynamic balance of bone formation and bone resorption. Mg²⁺ has been shown to increase osteoblast (OB) activity while suppressing osteoclast (OC) formation, thus playing a crucial role in bone remodelling and regeneration. In terms of osteolysis inhibition, Mg²⁺ plays a multifaceted role. First, Mg²⁺ inhibits OC formation by modulating the activity of mature OCs, their migratory behaviour and the activity of precursor cells. Second, Mg²⁺ influences OC production by regulating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor kappa-Β ligand (RANKL) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Additionally, Mg²⁺ impacts bone resorption by altering the immune microenvironment and the levels of hormones and peptides within the body. Furthermore, the alkaline environment generated around the biodegradable magnesium implant and its degradation products (e.g. H₂) also significantly inhibit OC formation. Recent research on magnesium-based implants has focused predominantly on their osteogenic properties, with few systematic reviews addressing the mechanisms through which biodegradable magnesium alloys suppress osteoclastic activity. This article summarizes the latest clinical research progress concerning biodegradable magnesium implant materials and their significant regulatory effects and discusses recent advances in the understanding of the regulatory mechanisms of action Mg-based biomaterials on OCs, with the aim of providing a more theoretical basis for the clinical application of biodegradable magnesium-based implants.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf026"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-inspired lotus-fiber and mussel-based multifunctional hydrogels for wound healing: super-stretchability, self-healing, adhesion and antibacterial properties.","authors":"Xiaoling Yang, Chenchen Li, Bo Li, Yuanyuan Zhang, Jinping Li, Na Liu, Xin Nie, Dawei Zhang, Ming Zhou, Xiaoling Liao","doi":"10.1093/rb/rbaf031","DOIUrl":"10.1093/rb/rbaf031","url":null,"abstract":"<p><p>Hydrogel-based wound dressings, which facilitate rapid wound closure and healing, are essential for effective wound management. However, the development of an ideal hydrogel that possesses excellent mechanical properties, effective self-healing capabilities, tissue adherence and antimicrobial characteristics for wound dressing presents a significant challenge in clinical settings. Inspired by lotus-fiber and mussel, we synthesized a novel multifunctional hydrogel composed of bacterial cellulose-reinforced dopamine-grafted oxidized hyaluronic acid/polyacrylamide (OHA-DA/PAM/BC). This was achieved through a one-pot reaction that employed free radical polymerization of acrylamide, dynamic Schiff bonding and intermolecular hydrogen bonding. Compared with the pure PAM hydrogels, which exhibited an elongation at break of 4022% and a maximum tensile strength of 26.42 kPa, the OHA-DA/PAM hydrogel demonstrated significantly enhanced stretchability at 9949% and an increased tensile strength of 34.73 kPa when 0.3% OHA-DA was incorporated during hydrogel formulation. Notably, the addition of 0.8% BC significantly enhanced the tensile strength to 57.04 kPa and super-stretchability to 10679%. The OHA-DA/PAM/BC hydrogel also exhibited remarkable self-healing capabilities, achieving a mechanical recovery of 84.74% within 12 h. Additionally, its adhesive and injectable properties are advantageous for dynamic wound repair. Furthermore, the OHA-DA/PAM/BC hydrogel exhibited minimal hemolytic activity and potent intrinsic antibacterial properties against both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. In a mouse model of wound healing, this hydrogel reduced the healing duration to 14 days while enhancing the regeneration of both skin structure and function. Histological analyses further revealed that the hydrogel significantly promoted the development of well-organized granulation tissue, angiogenic tissue and collagen accumulation in the wound region. This study successfully developed an OHA-DA/PAM/BC multifunctional hydrogel characterized by exceptional stretchability, self-healing, adhesiveness, injectability and antibacterial activity, demonstrating a significant impact on wound healing <i>in vivo</i>. These findings indicated that the OHA-DA/PAM/BC hydrogel holds substantial potential as wound dressings for future clinical applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf031"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural product-based nano-antioxidant for the treatment of acute pancreatitis.","authors":"Xiaoluan Lu, Ze Gao, Yanling Yu, Lang Zhang, Jing Huang, Xiaoming Zhang, Pei Jing, Shiyong Zhang, Mei Zeng","doi":"10.1093/rb/rbaf012","DOIUrl":"10.1093/rb/rbaf012","url":null,"abstract":"<p><p>Acute pancreatitis (AP) is a potentially highly fatal inflammatory disease characterized by the generation of high level of reactive oxygen species (ROS) of mass recruited inflammatory macrophages in pancreatic tissue. Many natural product antioxidants have been explored to treat AP due to their superiority in biosafety while the therapeutic application is restricted by their low ROS elimination as well as the rapid metabolism caused by small molecular weight and fast absorption. Herein, a new natural product-based nano-antioxidant (FA@zein-CS) that can overcome these problems was developed for the treatment of AP by encapsulating ferulic acid (FA) into the zein based nanoparticles and then hybridizing of chondroitin sulfate (CS). The FA@zein-CS would not only efficiently target to the inflamed pancreatic tissue by the specific binding of CS to CD44, but also effectively initiate the release of FA and zein degradation product in response to intracellular pH/GSH/ROS to achieve synergistic antioxidant effect. In addition, thanks to the fact that all components were derived from natural products, the FA@zein-CS held the excellent biocompatibility. <i>In vivo</i> results disclosed that the FA@zein-CS significantly reduced pancreatic structural damage and restored the pancreatic function with serum amylase and lipase reduced by 61.8% and 82.8%, respectively. This natural product-based nano-antioxidant holds great clinic potential for AP.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf012"},"PeriodicalIF":5.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vivo</i> dynamic visualization and evaluation of collagen degradation utilizing NIR-II fluorescence imaging in mice models.","authors":"Shunyao Li, Kai Xu, Huaixuan Sheng, Huizhu Li, Xiao Zhang, Chengxuan Yu, Haichen Hu, Xiner Du, Yunxia Li, Yu Dong, Jun Chen, Sijia Feng","doi":"10.1093/rb/rbaf025","DOIUrl":"10.1093/rb/rbaf025","url":null,"abstract":"<p><p>Collagen-based biomaterials are gaining prominence in tissue engineering, attributed to their remarkable biocompatibility, inherent biodegradability, and unparalleled capacity to facilitate tissue repair and regeneration. However, the ability to dynamically visualize and quantitatively assess collagen degradation <i>in vivo</i> remains a critical challenge, hindering the development of optimized biomaterials for clinical applications. To address this, a novel approach was developed to monitor the injury microenvironment by conjugating second near-infrared quantum dots with solid collagen. This live imaging system offered high-resolution, real-time tracking of collagen degradation both <i>in vitro</i> and <i>in vivo</i>, enabling a deeper understanding of the degradation behavior under various conditions. This system was applied to mouse models with different cartilage defects, including critical-sized defect (CSD), minor defect (Minor) and sham surgery (Sham) groups for a 28-day <i>in vivo</i> monitoring. Among them, the CSD group exhibited the fastest and most stable collagen degradation, indicating that the degradation rate was closely linked to the severity of the injury. Transcriptomic analysis further identified key signaling pathways that might drive rapid collagen degradation by promoting collagenase activity and tissue remodeling in cartilage defect conditions. In summary, our study provided valuable insights into the mechanisms of collagen degradation under different injury conditions, contributing to innovative strategies for designing collagen-related biomaterials in the future.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf025"},"PeriodicalIF":5.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials-based approaches to mandibular tissue engineering: where we were, where we are, where we are going.","authors":"Maeve M Kennedy, Antonios G Mikos","doi":"10.1093/rb/rbaf024","DOIUrl":"https://doi.org/10.1093/rb/rbaf024","url":null,"abstract":"<p><p>The mandible is the largest craniofacial bone and plays a crucial role in speech, mastication, swallowing, and facial aesthetics. The form or function of the mandible can be altered by defects as a result of tumors, trauma, infection, and congenital conditions. This paper covers the evolution of biomaterials-based approaches to the reconstruction of critical size mandibular defects. Historically the gold standard for critical size mandibular defect repair has been autologous fibula grafts. The emergence of the field of tissue engineering has led to the current research on biomaterial scaffolds, cells, and biological factors to design highly tunable, bio-inspired, tissue regenerative implants. Scaffold materials can be synthetic or natural and can be fabricated using a variety of additive manufacturing techniques. Mesenchymal stem cells, bone morphogenetic proteins, and transforming growth factor-β are frequently added to scaffolds. While great progress has been made, there are still barriers to translating this research to patients, ranging from insufficient bone regeneration in animal studies to the feasibility of establishing a good manufacturing practice. To address these challenges, the future of mandibular tissue engineering will look toward improving implant vascularization and innervation, personalizing implant shape and biology, and enhancing spatiotemporal control of drug release. With these goals in mind, researchers will ultimately develop biomaterials that can regenerate bone that is structurally and biologically identical to native mandibular tissue, improving both function and quality of life for patients.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf024"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-yield BMSC-derived exosomes by the 3D culture system to enhance the skin wound repair.","authors":"Jie Wu, Siqi Li, Hao Wang, Yuanbo Qi, Sheng Tao, Peifu Tang, Daohong Liu","doi":"10.1093/rb/rbaf022","DOIUrl":"https://doi.org/10.1093/rb/rbaf022","url":null,"abstract":"<p><p>Wound defects pose a substantial challenge in clinical practice, often resulting in prolonged healing times and an elevated risk of infection. Insufficient vascularization is a critical factor that adversely affects wound healing. Exosomes obtained from bone mesenchymal stem cells (BMSC-exos) have demonstrated significant promise in accelerating tissue repair by promoting angiogenesis. However, their limited yield and suboptimal biological functions impede widespread clinical application in enhancing wound healing. Prior research has indicated that 3D cultures can boost exosome secretion when compared to conventional 2D cultures. However, the currently prevalent 3D culture methods often necessitate expensive equipment or cumbersome procedures. This study investigates a cost-effective and user-friendly 3D culture system developed using gelatin methacrylate (GelMA). Our findings indicate that a 5% concentration of GelMA provides an optimal environment for the 3D culture of BMSCs. Furthermore, we observed that 3D culture significantly delays the senescence of BMSCs, thereby creating favorable conditions for the sustained production of exosomes. Additionally, 3D cultivation has the potential to boost exosome secretion and enhance their angiogenic capabilities. <i>In vivo</i> experiments further confirmed that BMSC-exos from a 3D environment exhibit enhanced capabilities to promote wound healing. These results suggest that GelMA-based 3D cultures offer a novel strategy for both industrial production and clinical application of exosomes.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf022"},"PeriodicalIF":5.6,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimensional effects of surface morphology and trapped air on mammalian cell adhesion to special wetting surfaces.","authors":"Zhiwei Chen, Yun Yang, Shaohua Xu, Zhenyu Shen, Yijian Tang, Yisheng Lin, Qiaoling Huang","doi":"10.1093/rb/rbaf021","DOIUrl":"https://doi.org/10.1093/rb/rbaf021","url":null,"abstract":"<p><p>Materials with special wettability have broad biomedical applications, including the control of mammalian cell adhesion and inhibiting biofilm formation. However, limited understanding of mammalian cellular responses to superhydrophobic materials with trapped air restricts their clinical applications. In this study, we fabricated materials with varied nanostructures and wettability, and systematically compared short-term mammalian cellular responses in the presence and absence of trapped air. Our results show that small nanostructures generate small, often invisible air bubbles at the solid-liquid interface when in contact with mammalian cell suspensions. In the presence of these small bubbles, the number of adhered cells was comparable to both the same sample without trapped air and its hydrophilic counterpart, contradicting the intuitive expectations that trapped air would reduce cell adhesion. In contrast, larger nanostructures resulted in visible, hundred-micron-sized air bubbles, which significantly inhibited cell adhesion. This effect was evident when comparing the same superhydrophobic sample with and without trapped air, as well as against hydrophilic counterparts with the same morphology. Further tracking of large air bubbles on the hydrophobic materials revealed that no cells adhered to the areas occupied by hundred-micron-sized air bubbles, while more cells accumulated at the solid-liquid-gas triple line. Hence, this work deepens the understanding of cellular responses to superhydrophobic materials, revealing that material structure size influences the size of trapped air and subsequently dominates cell adhesion.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf021"},"PeriodicalIF":5.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12017620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart materials strategy for vascular challenges targeting in-stent restenosis: a critical review.","authors":"Kai Zhang, Wenzhao Liang, Xiao-Bo Chen, Jing Mang","doi":"10.1093/rb/rbaf020","DOIUrl":"https://doi.org/10.1093/rb/rbaf020","url":null,"abstract":"<p><p>In-stent restenosis (ISR) presents a major challenge in vascular disease management, often leading to complications and repeated interventions. This review article explores the potential of existing smart materials strategies in addressing ISR, emphasizing advancements in materials science and biomedical engineering. We focus on innovative solutions such as bioactive coatings and responsive polymers that offer targeted responses to ISR-related internal and external triggers. These smart materials can dynamically adapt to the physiological conditions within blood vessels, responding in real time to various stimuli such as pH, oxidative stress and temperature. Moreover, we discuss preclinical progress and translational challenges associated with these materials as they move toward clinical applications. The review highlights the importance of controlled drug release and the need for materials that can degrade appropriately to minimize adverse effects. This work aims to identify critical research gaps and provide guidance to encourage interdisciplinary efforts to advance the development of smart stent technologies. Ultimately, the goal is to improve patient outcomes in vascular interventions by leveraging the capabilities of intelligent biomaterials to enhance ISR management and ensure better long-term efficacy and safety in-stent applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf020"},"PeriodicalIF":5.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical methods in studying cell force sensing: principles, current technologies and perspectives.","authors":"Xiaojun Liu, Lei Yu, Adam Xiao, Wenxu Sun, Han Wang, Xiangxiu Wang, Yanghao Zhou, Chao Li, Jiangtao Li, Yongliang Wang, Guixue Wang","doi":"10.1093/rb/rbaf007","DOIUrl":"https://doi.org/10.1093/rb/rbaf007","url":null,"abstract":"<p><p>Mechanical stimulation plays a crucial role in numerous biological activities, including tissue development, regeneration and remodeling. Understanding how cells respond to their mechanical microenvironment is vital for investigating mechanotransduction with adequate spatial and temporal resolution. Cell force sensing-also known as mechanosensation or mechanotransduction-involves force transmission through the cytoskeleton and mechanochemical signaling. Insights into cell-extracellular matrix interactions and mechanotransduction are particularly relevant for guiding biomaterial design in tissue engineering. To establish a foundation for mechanical biomedicine, this review will provide a comprehensive overview of cell mechanotransduction mechanisms, including the structural components essential for effective mechanical responses, such as cytoskeletal elements, force-sensitive ion channels, membrane receptors and key signaling pathways. It will also discuss the clutch model in force transmission, the role of mechanotransduction in both physiology and pathological contexts, and biomechanics and biomaterial design. Additionally, we outline analytical approaches for characterizing forces at cellular and subcellular levels, discussing the advantages and limitations of each method to aid researchers in selecting appropriate techniques. Finally, we summarize recent advancements in cell force sensing and identify key challenges for future research. Overall, this review should contribute to biomedical engineering by supporting the design of biomaterials that integrate precise mechanical information.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf007"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable polymeric occluder with controllable locking structure for closure of atrial septal defect via interventional treatment.","authors":"Daokun Shi, Yahong Kang, Weijie Wang, Ruili Liu, Quansheng Tang, Zhaomin Li, Hongyan Jiang, Jiandong Ding","doi":"10.1093/rb/rbaf016","DOIUrl":"https://doi.org/10.1093/rb/rbaf016","url":null,"abstract":"<p><p>Atrial septal defect (ASD) is one of the major congenital heart diseases, and transcatheter closure with a cardiac occluder is a modern method to treat ASD with the advantage of mini-invasiveness over traditional surgical closure. While current occlusion devices are mainly made of non-degradable nitinol with superelasticity, the permanent existence of a metal <i>in vivo</i> may trigger potential complications and especially has an adverse effect on the heart development for children. However, it is challenging to invent a superelasticity-free occluder that can be delivered through a catheter but firmly locked after being opened at the target site; it is also much desired for research and development to quickly assess the feasibility of a superelasticity-free occluder <i>in vitro</i>. Herein, a biodegradable poly(L-lactide) (PLLA) occluder composed of a braided PLLA frame as the skeleton and a nonwoven PLLA fabric as the flow-blocking membrane is developed, and a controllable locking structure is designed to enable firm closure for a device even without superelasticity. We also suggest and justify a series of <i>in vitro</i> methods to assess the efficacy of the biodegradable occluder, and the results confirm the reliability of locking, water-blocking, mechanical strength and degradability. It is found that the PLLA fabric with moderate fiber density is optimal for surface endothelialization. We also carry out biological assessments; significant endothelialization and alleviated inflammation response are observed after 6 months of subcutaneous implantation into rabbits. The porcine model illustrates that the biodegradable polymeric occluder can be successfully implanted into the atrial septum via transcatheter intervention; the follow-ups have confirmed the safety and efficacy of this biodegradable polymeric occluder with the controllable locking structure.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf016"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12005900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}