Regenerative Biomaterials最新文献

{"title":"Recombinant human fibronectin segment (rhFN₁₀₂₄) hydrogel carried hPDLSCs to repair diabetic trauma by activated NF-κB signaling pathway.","authors":"Jianhang Cong, Yating Cheng, Tongtong Liu, Xiang Cai, Jiahui Xu, Rui Guo, Rongrong He, Qi Xiang","doi":"10.1093/rb/rbaf027","DOIUrl":"10.1093/rb/rbaf027","url":null,"abstract":"<p><p>The accumulation of advanced glycation end products (AGEs) plays a crucial role in chronic inflammation and delayed wound healing in individuals with diabetes. In this context, fibronectin has been identified as a crucial protein that promotes the differentiation of human periodontal ligament stem cells (hPDLSCs) into myofibroblasts, which play a vital role in the repair of diabetic skin ulcers. This process is intimately associated with the integrin β1 receptor and the NF-κB signaling pathway, both crucial for cellular responses to fibronectin. To validate our hypothesis, we expressed rhFN₁₀₂₄, a recombinant protein containing the integrin β1 affinity-binding domain from human fibronectin segments 12-14. This protein was used to formulate a hydrogel for hPDLSCs. rhFN₁₀₂₄'s binding affinity to integrin β1 was confirmed by molecular docking and the cellular thermal shift assay (CETSA). We developed <i>sh-ITGB1</i>-hPDLSCs with stable ITGB1 knockdown using <i>shRNA-ITGB1</i> and compared their proliferation, migration and adhesion to wild-type hPDLSCs. Morphological changes were observed via SEM, and α-SMA expression levels were measured in AGEs-damaged hPDLSCs. We created full-thickness wound models in diabetic mice to assess pharmacodynamics. The study showed that rhFN₁₀₂₄ stimulated hPDLSCs differentiation into myofibroblasts by boosting ITGB1 expression. rhFN₁₀₂₄ also reduced AGEs' negative effects on hPDLSCs, as seen through SEM analysis and α-SMA levels. In full-thickness wound models, hPDLSCs and rhFN₁₀₂₄ accelerated re-epithelialization and collagen synthesis. rhFN₁₀₂₄ is proposed to interact with the ITGB1 receptor on hPDLSCs, activating the NF-κB pathway to neutralize AGEs-induced pro-inflammatory cytokines. This study suggests rhFN₁₀₂₄ as a potential biomedical material for tissue repair.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf027"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Y-shaped DNA as a dynamic self-assembly nanomaterial for phenotype-specific regulation of stem cell differentiation on the gene level.","authors":"Wengang Liu, Ruili Liu, Lok Ting Chu, Xinlei Wang, Jianpeng Wu, Jiandong Ding, Ting Hsuan Chen","doi":"10.1093/rb/rbaf043","DOIUrl":"10.1093/rb/rbaf043","url":null,"abstract":"<p><p>While genetic engineering has offered new strategies for regulating stem cell differentiation, the efficacy varies in cells with different phenotypes or lineage commitments, leading to inconsistent differentiation outcomes and uncertainty in regenerative medicine. To address this issue, we employ a Y-shaped DNA (Y-DNA) as a nanomaterial to phenotype-specifically regulate differentiation of human mesenchymal stem cells (hMSCs). Y-DNA is composed of three DNA strands with complementary sequences and different roles. The Y-DNA designed in the present study can be uniquely activated by miR-106a-5p, a microRNA preferentially expressed in adipogenesis-biased hMSCs. Upon activation, the Y-DNA disassembles, releasing an antisense oligonucleotide that inhibits expression of cofilin, which serves as a key regulator to enhance adipogenic differentiation, and thus, prevents hMSCs from undergoing osteogenic differentiation. The key regulatory role of cofilin in hMSC differentiation is verified at the single-cell level on arginine-glycine-aspartate microislands under the nonfouling background of poly(ethylene glycol) hydrogels. Our strategy effectively redirects these cells towards osteogenic differentiation by inhibiting adipogenic differentiation, demonstrating dose dependence with high specificity, selectivity, and low toxicity. hMSCs cultured in a dual induction medium (a mixture of adipogenic medium and osteogenic medium) show enhanced osteogenic differentiation after transfection with the nanostructured Y-DNA. This approach addresses the challenge of cell heterogeneity in bone regeneration, offering a promising solution for precise control over stem cell fate. The ability of Y-DNA to specifically target cells with a propensity for adipogenic differentiation and to reprogram their lineage commitment has significant implications for the field of regenerative medicine, particularly in applications requiring enhanced purity of cell differentiation outcomes.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf043"},"PeriodicalIF":5.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced prevention on postoperative atrial fibrillation by using anti-inflammatory biodegradable drug patch.","authors":"Pengcheng Yu, Weiqi Lu, Huaxin Sun, Chengchen Huang, Xiaolin Zhou, Yuxing Wang, Zhen Zhang, Guosheng Fu, Hanxiong Liu, Kefeng Ren, Xia Sheng","doi":"10.1093/rb/rbaf040","DOIUrl":"10.1093/rb/rbaf040","url":null,"abstract":"<p><p>Postoperative atrial fibrillation (POAF) is the most prevalent form of secondary atrial fibrillation and increases the risk of adverse cardiovascular outcomes, such as stroke, heart failure and increased mortality. Herein, we designed an andrographolide (Andr)-loaded degradable polymer patch to deliver the drug directly to the atrial tissue for prevention of POAF. The sterile pericarditis (SP) rat model was adopted for highly relationship to clinical practice. The patch-released Andr effectively reduced the incidence of atrial fibrillation from 90 to 20%, and alleviated local atrial inflammation and oxidative stress <i>in vivo</i>, by using electrophysiological detection and histological analysis such as immunofluorescence, western blot and PCR. In HL-1 cells, we found the use of Andr-loaded patch could strongly inhibit the cell death, reactive oxygen species (ROS) generation and mitochondrial injury caused by LPS. Meanwhile, the use of Andr-loaded patch could effectively inhibited macrophages polarize towards M1. Mechanistically, we verified that the regulation of the cytoplasm and mitochondria Ca²⁺ and ROS dynamic balance was quite important both <i>in vivo</i> and <i>in vitro</i>. Our strategy proved by regulating the inflammatory microenvironment, ROS balance and Ca²⁺ homeostasis and the Andr-loaded atrial patch was effective for POAF in the SP rat model. The electrical signal of atrial stromal reentry in the case of this model was successfully mined, and the results of calcium channel were basically consistent with that of electrical signal channel. In addition, we have reported the infiltration and polarization of local inflammatory cells in the atrial of POAF at the tissue section level. Our study served as a new inspiration for the treatment of arrhythmic diseases and other ROS- and Ca²⁺- associated local illnesses.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf040"},"PeriodicalIF":5.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design matrix materials for organoid development and application in biomedicine.","authors":"Yue Huang, Xiaoyu Zhang, Wanjun Zhang, Jinglong Tang, Jing Liu","doi":"10.1093/rb/rbaf038","DOIUrl":"10.1093/rb/rbaf038","url":null,"abstract":"<p><p>Organoids are three-dimensional tissue analogues grown <i>in vitro</i>. Although they are not human organs in the strict sense, they can mimic the structure and function of tissues <i>in vivo</i> to the maximum extent, and have broad application prospects in the fields of organ development, personalized medicine, regenerative medicine, disease modeling, drug screening, gene editing, etc. There is even hope that organoids can replace experimental animals for preclinical testing, which will greatly shorten the cycle of preclinical testing and improve its efficiency. Nowadays, Matrigel remains the predominant substitute for organoid culture systems. At the same time, new extracellular matrix or inspired polymer materials with tunable and optimized biochemical and biophysical properties continue to emerge, which are of great significance for efficient and high-level cultivation of organoids. In this review, we critically evaluate how mechanobiological signaling dynamics at the cell-matrix interface inform the rational engineering of biomimetic extracellular matrices to achieve standardized and phenotypically regulated patient-derived organoid cultures. Then, we systematically classify hydrogel-based matrices encompassing natural, biohybrid, synthetic, protein-engineered and DNA crosslinked matrix systems by their biocompatibility and functional compatibility. Focusing on cancer oncogenesis and progression research, drug development and personalized medicine, we highlight biomimetic hydrogel innovations that recapitulate tumor organoids development. By summarizing the obstacles that hinder the development of organoid hydrogels, we hope to provide an outlook on the future directions for the development of organoid hydrogels and promote the application of organoids in the field of biomedicine.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf038"},"PeriodicalIF":5.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel iron bioresorbable scaffold: a potential strategy for pulmonary artery stenosis.","authors":"Li Qin, Gui Zhang, Ling Sun, Zhijin Yu, Zhe Zhang, Lifeng Sun, Wanqian Zhang, Wenchao Fu, Yetao Ou, Wenjing Zhang, Xiaoli Shi, Zhixiang Si, Jingfang Shen, Limei Cha, Zhiwei Zhang, Deyuan Zhang","doi":"10.1093/rb/rbaf041","DOIUrl":"10.1093/rb/rbaf041","url":null,"abstract":"<p><p>A big diameter bioresorbable scaffold is expected to be used for treatment of vessel stenosis of children with congenital heart disease to adapt the growth characteristics of vessel of children and avoid the late adverse events of permanent stent implanted in children. However, it is challenging to fabricate a big diameter bioresorbable scaffold that is appropriate for percutaneous implantation with enough mechanical performance and can be smoothly delivered in children's small vessel. In this study, a novel iron big and bioresorbable Scaffold (BBS) for pulmonary artery stenosis of children with congenital cardiovascular diseases was fabricated and evaluated. The BBS was made of nitrided iron tube and processed by laser cutting and polishing. The testing results of radial strength, recoil, shortening, maximal expansion diameter and side-branch accessability illustrated the BBS has good mechanical performance. The animal study showed that the percentage of area stenosis of BBSs was 18.1 ± 8.6%, 20.2 ± 5.9% and 20.4 ± 6.1% at 28, 90 and 180 days after implantation in 17 rabbits, and no malposition, thrombus, dissection or tissue necrosis in the rabbit model was detected by micro-CT, STEM and histological examinations. An φ8 × 23 mm BBS was implanted into a 55-month-old child with left pulmonary stenosis, and multiple spiral CT was conducted. No lumen area loss appeared at 1- and 2-year follow-ups in this first-in-man study. It suggested that the BBS might be a new strategy for the therapy of pulmonary artery stenosis in children.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf041"},"PeriodicalIF":5.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable hydrogel loaded with exosomes from hypoxic umbilical cord-derived mesenchymal stem cells alleviates intervertebral disc degeneration by reversing nucleus pulposus cell senescence.","authors":"Xin Zhao, Yubo Shi, Zhen Sun, Wei Duan, Le Chang, Benchi Xu, Kangwei Lai, Jingchun Zhang, Buqi Tian, Weidong Tao, Zhenzhou Mi, Mian Zhang, Wenjing Yang, Zhuojing Luo, Zhengxu Ye","doi":"10.1093/rb/rbaf039","DOIUrl":"10.1093/rb/rbaf039","url":null,"abstract":"<p><p>Intervertebral disc degeneration is a significant contributor to the development of spinal disorders. Previous studies have shown that the senescence of nucleus pulposus cells can worsen the degradation of intervertebral disks. Therefore, targeting the senescence of nucleus pulposus cells may be a promising therapeutic approach for the treatment of intervertebral disc degeneration. This study investigated the use of exosomes from hypoxic umbilical cord-derived mesenchymal stem cells to reverse nucleus pulposus cells senescence and delay intervertebral disc degeneration progression. MicroRNA sequencing of hypoxic umbilical cord-derived mesenchymal stem cells revealed the presence of functional microRNAs, with the p53 signalling pathway identified as a key factor. To enhance the release time of hypoxic umbilical cord-derived mesenchymal stem cells <i>in vivo</i>, hyaluronic acid methacryloyl hydrogel was used to load hypoxic umbilical cord-derived mesenchymal stem cells and create a sustained-release system. This system effectively repaired the degradation of the extracellular matrix, reversed nucleus pulposus cells senescence and alleviated intervertebral disc degeneration progression in a rat model. Overall, this study highlights the potential of hypoxic umbilical cord-derived mesenchymal stem cells in reducing nucleus pulposus cell senescence and suggests the possibility of combining it with a sustained-release system as a novel therapeutic strategy for intervertebral disc degeneration.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf039"},"PeriodicalIF":5.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the maturity of <i>in vitro</i> engineered cartilage from Wharton's jelly-derived photo-crosslinked hydrogel using dynamic bioreactors and its <i>in vivo</i> outcomes in animal models.","authors":"Chuanzhi Wei, Mingyue Lin, Qitao Bo, Wufei Dai, Jinghao Ding, Ru Chen","doi":"10.1093/rb/rbaf037","DOIUrl":"10.1093/rb/rbaf037","url":null,"abstract":"<p><p>The immature state of <i>in vitro</i> engineered cartilage (IVEC) hinders its clinical translation, highlighting the need for optimized scaffold platforms and cultivation models. Our previous work demonstrated that Wharton's jelly (WJ) contains an extracellular matrix (ECM) whose composition closely resembles that of native cartilage and includes several bioactive factors that promote chondrogenic induction. Furthermore, earlier studies have shown that photo-crosslinkable hydrogels are ideal carrier scaffolds for cartilage tissue engineering and that bioreactors improve nutrient and waste exchange between scaffolds and the culture medium. Based on these findings, we employed a dynamic bioreactor in combination with a WJ-derived photo-crosslinkable hydrogel to enhance IVEC maturity. Our results indicate that the decellularized WJ matrix (DWJM) effectively retains its native chondrogenic ECM components and bioactive factors. The photo-crosslinkable ADWJM hydrogel-produced by modifying DWJM with methacrylate anhydride-demonstrated excellent gelation capacity as well as tunable rheological properties, swelling ratios and degradation rates across different DWJM concentrations. In addition, the ADWJM hydrogel exhibited outstanding biocompatibility by providing a favorable 3D microenvironment for chondrocyte survival and proliferation. Most importantly, the dynamic bioreactor markedly promoted IVEC maturation. Constructs cultured under dynamic conditions displayed increased thickness, wet weight and volume; enhanced mechanical strength; more typical lacunae structures; and uniform deposition of cartilage-specific ECM compared to constructs maintained in static conditions or within a static bioreactor. Moreover, in vivo subcutaneous implantation of IVEC in goats further validated these findings, as the implanted constructs exhibited cartilage components and mechanical properties closely resembling those of natural cartilage. These results offer a promising approach for enhancing IVEC maturity and support its future clinical translation.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf037"},"PeriodicalIF":5.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An acid-responsive bone-targeting nanoplatform loaded with curcumin balances osteogenic and osteoclastic functions.","authors":"Minhao Liang, Lei Zhou, Juan Li, Bin Liang, Liangyun Zhou, Fengfeng Xue, Libo Jiang, Wei Hong","doi":"10.1093/rb/rbaf028","DOIUrl":"10.1093/rb/rbaf028","url":null,"abstract":"<p><p>Postmenopausal osteoporosis (PMOP) is a predominant form of clinical osteoporosis. It has led to significant health and social burdens for older patients. Reestablishing the balance between osteogenic and osteoclastic is a crucial strategy for treating PMOP. Curcumin (Cur), a naturally derived polyphenolic substance, has gained recognition as a viable option for treating osteoporosis. Despite its potential, the clinical use of Cur is hindered by its limited bioavailability and the presence of side effects. Nanoparticles modified with aspartic acid octapeptide (ASP8) exhibit a strong affinity for bone tissue, facilitating targeted delivery. This study presents novel acid-responsive zeolite imidazolate framework-8 (ZIF) nanoparticles modified with ASP8 and loaded with Cur (Cur@ZIF@ASP8, CZA). Upon delivery by this nanoparticle drug delivery system, Cur can effectively regulate bone homeostasis, offering a potential therapeutic strategy for osteoporosis. This study demonstrated that CZA nanoparticles could successfully transport Cur to bone tissue without significant toxicity. Furthermore, nanoparticles promote bone formation and inhibit osteoclast activity. They also modify the expression of related genes and proteins, such as OCN, ALP, CTSK and MMP9. Significant evaluations utilizing microcomputed tomography, Masson's staining, hematoxylin and eosin staining and immunofluorescence staining demonstrated that intravenous CZA administration in ovariectomized mice resulted in bone destruction while simultaneously reducing overall bone loss. In conclusion, CZA nanoparticles hold promise as a therapeutic option for osteoporosis.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf028"},"PeriodicalIF":5.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed Gel/PTH@PAHA scaffolds with both enhanced osteogenesis and mechanical properties for repair of large bone defects.","authors":"Zhimou Zeng, Ping Song, Xingyu Gui, Bicheng Ake, Taoyu Liu, Hao Liu, Linnan Wang, Lei Wang, Yueming Song, Bo Qu, Changchun Zhou","doi":"10.1093/rb/rbaf029","DOIUrl":"10.1093/rb/rbaf029","url":null,"abstract":"<p><p>The repair of large bone defects continues to pose a significant challenge in clinical orthopedics. Successful repairs require not only adequate mechanical strength but also exceptional osteogenic activity for successful clinical translation. Composite materials based on polyamide 66 (PA66) and hydroxyapatite have been widely used in various clinical settings. However, existing PA66/hydroxyapatite composites often lack sufficient osteogenic stimulation despite their favorable mechanical properties, which limit their overall clinical efficacy. In this study, we fabricated a polyamide 66/nano-hydroxyapatite (PAHA) scaffold using an extruder and fused deposition modeling-based 3D printing technology. Subsequently, gelatin methacrylamide (GelMA) containing teriparatide (PTH) was incorporated into the PAHA scaffold to construct the Gel/PTH@PAHA scaffold. Material characterization results indicated that the compressive modulus of elasticity and compressive strength of the Gel/PTH@PAHA scaffold were 172.47 ± 5.48 MPa and 25.55 ± 2.19 MPa, respectively. <i>In vitro</i> evaluations demonstrated that the Gel/PTH@PAHA scaffold significantly enhanced osteoblast adhesion and proliferation while promoting osteogenic differentiation of BMSCs. <i>In vivo</i> studies further revealed that this scaffold notably promoted new bone regeneration in rabbit femoral defects. These findings suggest that the 3D-printed Gel/PTH@PAHA scaffold exhibits excellent mechanical properties alongside remarkable osteogenic activity, thereby meeting the dual requirements for load-bearing applications and bone regeneration. This innovative approach may be a promising candidate for customized orthopedic implants with substantial potential for clinical application.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf029"},"PeriodicalIF":5.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing electrospinning towards the future of biomaterials in biomedical engineering.","authors":"Yanjiao Teng, Lin Song, Jie Shi, Qi Lv, Shike Hou, Seeram Ramakrishna","doi":"10.1093/rb/rbaf034","DOIUrl":"10.1093/rb/rbaf034","url":null,"abstract":"<p><p>Biomaterial is a material designed to take a form that can direct, through interactions with living systems, the course of any therapeutic or diagnostic procedure. Growing demand for improved and affordable healthcare treatments and unmet clinical needs seek further advancement of biomaterials. Over the past 25 years, the electrospinning method has been innovated to enhance biomaterials at nanometer and micrometer length scales for diverse healthcare applications. Recent developments include intelligent (smart) biomaterials and sustainable biomaterials. Intelligent materials can sense, adapt to and respond to external stimuli, autonomously adjusting to enhance functionality and performance. Sustainable biomaterials possess several key characteristics, including renewability, a low carbon footprint, circularity, durability, biocompatibility, biodegradability and others. Herein, advances in electrospun biomaterials, encompassing process innovations, working principles and the effects of process variables, are presented succinctly. The potential of electrospun intelligent biomaterials and sustainable biomaterials in specific biomedical applications, including tissue engineering, regenerative medicine, drug delivery systems, brain-computer interfaces, biosensors, personal protective equipment and wearable devices, is explored. More effective healthcare demands further advancements in electrospun biomaterials. In the future, the distinctive characteristics of intelligent biomaterials and sustainable biomaterials, integrated with various emerging technologies (such as AI and data transmission), will enable physicians to conduct remote diagnosis and treatment. This advancement significantly enhances telemedicine capabilities for more accurate disease prediction and management.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf034"},"PeriodicalIF":5.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}