Regenerative Biomaterials最新文献

{"title":"Heterogeneous zinc/catechol-derived resin microsphere-functionalized composite hydrogels with antibacterial and anti-inflammatory activities promote bacterial-infected wound healing.","authors":"Lianyi Qu, Anle Yang, Yulei Shi, Jianglong Liu, Xueyan Li, Bohan Mao, Xiaoran Li, Fang Zhou, Yingjun Xu","doi":"10.1093/rb/rbaf081","DOIUrl":"10.1093/rb/rbaf081","url":null,"abstract":"<p><p>Bacterial infection in the injured skin may threaten the wound repair and skin regeneration owing to aggravated inflammation. The multifunctional dressings with persistent antibacterial activity and improved anti-inflammatory capability are urgently required. Herein, a type of heterogeneous zinc/catechol-derived resin microspheres (Zn/CFRs) composed of zinc ions (Zn²⁺) and zinc oxide (ZnO) nanoparticles was developed to impart the methacrylamide chitosan (CSMA)-oxidized hyaluronic acid (OHA) hydrogel with a persistent Zn²⁺ release behavior. The Zn/CFRs synthesized via a one-step hydrothermal method exhibited a Zn²⁺-enriched surface and internal ZnO nanoparticles. Owing to the unique microstructure of the microspheres, the Zn/CFRs-functionalized hydrogel (CH-ZnCFR) was able to rapidly release Zn²⁺ in the initial phase and sustain the release of Zn²⁺ for 14 days. Importantly, CH-ZnCFR exhibited excellent anti-inflammatory property by facilitating the macrophage polarization, and also effectively inhibited the growth of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. In addition, CH-ZnCFR showed excellent self-healing and tissue adhesion properties, and great cytocompatibility by improving fibroblast migration behavior <i>in vitro</i>. Moreover, CH-ZnCFR demonstrated outstanding therapeutic effects in a murine model of <i>S. aureus</i>-infected wounds, including effectively inhibiting bacterial growth, reducing inflammation, increasing the number of M2-type macrophages and facilitating collagen deposition, angiogenesis and tissue regeneration. Therefore, this Zn/CFRs-functionalized composite hydrogel represents a promising strategy for bacterial-infected wound healing and regeneration.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf081"},"PeriodicalIF":8.1,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection mechanism investigation of a protective coating on magnesium alloy stents via deformation model construction and the simulation of cellular automata.","authors":"Dexiao Liu, Hong Qin, Feng Zheng, Maoyu Zhao, Xiaohui Zhao, Wenhua Yan, Yingxue Teng, Shanshan Chen","doi":"10.1093/rb/rbaf084","DOIUrl":"10.1093/rb/rbaf084","url":null,"abstract":"<p><p>The most significant challenge facing magnesium alloy stents is their ability to withstand complex deformation during their application. To gain a deeper understanding of the impact of stent deformation on the protective capabilities of the coating, this paper presents an amplified stent deformation model. The models were coated with either a low elongation material-Poly(D, L-lactide) (PDLLA) or a high elongation material-Poly(butylene adipate-co-terephthalate) (PBAT), followed by the application of a rapamycin-loaded PLGA as drug-eluting layer. Coating integrity and thickness were examined via scanning electron microscopy (SEM), while electrochemical impedance spectroscopy and long-term immersion tests assessed corrosion behavior on the deformation model. Finite element analysis using Comsol simulated the stress-strain distribution during compression and tension, and cellular automata (CA) models were employed to simulate the corrosion process. The drug release tests were conducted <i>in vitro</i>, and <i>in vivo</i> performance was evaluated through stent implantation in rabbit carotid arteries using optical coherence tomography, SEM, and histological analysis. Results demonstrated that PBAT coatings maintained structural integrity without apparent microcracks after deformation, whereas PDLLA coatings exhibited significant cracking and significantly reduced charge transfer resistance. This reduction in protective performance is observed to occur predominantly in regions of strain concentration with more porosity during the deformation process. CA simulations and immersion tests confirmed slower degradation rates under PBAT. Moreover, PBAT-coated stents achieved larger luminal areas, reduced neointimal formation, and lower restenosis rates compared to PDLLA-coated counterparts <i>in vivo</i>. In conclusion, PBAT coatings offer robust protection against deformation-induced damage and corrosion, representing a promising strategy for enhancing the long-term performance of Mg alloy stents.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf084"},"PeriodicalIF":8.1,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix metalloproteinase-2 and pH-responsive drug eluting multilayer as intraocular lens coating to improve the posterior capsule opacification inhibition.","authors":"Yuemei Han, Jiahao Wang, Hao Chen, Quankui Lin","doi":"10.1093/rb/rbaf077","DOIUrl":"10.1093/rb/rbaf077","url":null,"abstract":"<p><p>Intraocular lens (IOL) is a crucial implant for cataract therapy. Posterior capsule opacification (PCO) is the most common postoperative complication after IOL implantation, which is the abnormal hyperplasia of the residual lens epithelial cells (LECs) after IOL implantation in cataract surgery. It is reported that the cellular microenvironment in the lens capsule changes after surgery, such as the elevated secretion of matrix metalloproteinases (MMPs) and a decrease in pH due to undesired cell proliferation. In this study, MMP-2 and pH-triggered drug delivery polysaccharide multilayer coating was designed and introduced onto the IOL surface for obtaining the cellular microenvironment-sensitive drug-eluting intraocular implant. The methacrylated heparin (HEP-MA) was synthesized and used to layer-by-layer self-assemble with the doxorubicin-loaded chitosan nanoparticles on the IOL surface. The matrix metalloproteinase-2 (MMP-2) sensitive peptide with cysteine contained in both ends (GCRD-GPQGIWGQ-DRCG) was then used to crosslink the polysaccharide multilayer via the Michael addition reaction between sulfhydryl group in cysteines and double bonds in methacrylate groups. The multilayer construction and subsequent cross-linking were validated through ultraviolet-visible spectrophotometer (UV-Vis) and Fourier transform infrared spectroscopy (FTIR). After modification, the IOL material surface becomes more hydrophilic while the optical properties were well maintained. The MMP-2 and pH-sensitive drug sustained-release coating were successfully obtained on the IOL surface via such design. The enzyme-triggered cell proliferation inhibition was realized in the <i>in vitro</i> experiments. In an animal model, significant up-regulation of MMP-2 was observed in the aqueous humor after cataract surgery. The multi-functionalized polysaccharide-coated IOL implanted in the animal eye via cataract surgery effectively inhibits PCO formation while it keeps good <i>in vivo</i> biosafety.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf077"},"PeriodicalIF":8.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant human collagen type III microgel: an advanced injectable dermal filler for rejuvenating aging skin.","authors":"Yafang Chen, Yihan Zhao, Xinyue Zhang, Yang Sun, Kang Li, Liguo Zhang, Shuang Li, Jie Liang, Kefeng Wang, Yujiang Fan","doi":"10.1093/rb/rbaf076","DOIUrl":"10.1093/rb/rbaf076","url":null,"abstract":"<p><p>Skin aging, characterized by reduced collagen regeneration, chronic inflammation and heightened skin cancer risk, poses a significant challenge. Collagen-based materials, employed as dermal fillers to smooth wrinkles, have attained extensive utilization. Nevertheless, traditional animal-derived collagen protein primarily presents concerns pertaining to disease risks, potential immunological reactions, and batch instability. Here, we introduced a highly durable 1,4-butanediol diglycidyl ether cross-linked recombinant human collagen type III (rhCol III) microgel as dermal filler for rejuvenating aging skin. The rhCol III microgel exhibited exceptional thermostability, mechanical strength and injectability. Subsequently, we established a UV-photoaging skin animal model and chose rhCol III microgel as a bioactive material for <i>in vivo</i> implantation, systematically comparing its biological effect with commercialized collagen I (Col I) derived from porcine skin (pCollagen) and hyaluronic acid through histological observation, immunofluorescence staining, hydroxyproline quantification and analysis of specific gene expression. Outcomes indicated rhCol III microgel prompted augmented production of Col I, collagen III (Col III) and elastic fibers, thereby contributing to the remodeling of the extracellular matrix (ECM). In summary, our investigation contributed to robust biosafety and rejuvenation of UV-induced skin photoaging by rhCol III under a single injection for 6 weeks. Despite the imperative ongoing efforts required for the successful translation from bench to clinic, the discernibly superior safety and efficacy of rhCol III microgel present an innovative methodology in combating skin aging, offering significant promise in medical cosmetology and tissue engineering.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf076"},"PeriodicalIF":8.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12368855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnesium phosphate functionalized graphene oxide and PLGA composite matrices with enhanced mechanical and osteogenic properties for bone regeneration.","authors":"Taraje Whitfield, Fatemeh S Hosseini, Jason D Orlando, Chenyun Deng, Kevin W-H Lo, Ho-Man Kan, Debolina Ghosh, Stefanie A Sydlik, Cato T Laurencin","doi":"10.1093/rb/rbaf074","DOIUrl":"10.1093/rb/rbaf074","url":null,"abstract":"<p><p>Bone defects affect millions of people annually, making bone tissue of particular interest for developing treatments. Current strategies for healing suffer drawbacks. Regenerative engineering seeks to achieve efficient bone regeneration by utilizing synthetic bone grafts to evade these drawbacks. One material that offers such benefits is a class of functional graphenic material, known as Phosphate Graphenes. While many of our studies have focused on Calcium Phosphate Graphene, magnesium is also osteogenic. Therefore, in this study, we utilized regenerative engineering techniques to incorporate Magnesium Phosphate Graphene (MgPG) into poly(lactic-co-glycolic acid) (PLGA) to fabricate composite microsphere-based matrices as a potential synthetic bone graft. Employing different amounts of MgPG within PLGA matrices, we studied the effect of MgPG on the morphological, structural, physical and biological characteristics. MgPG-containing matrices demonstrated great mechanical strength, hydrophilicity and degradability without compromising matrix morphology. Because MgPG is a graphene oxide derivative with magnesium and phosphate ions capable of supporting bone healing as inducerons, we next evaluated the cytocompatibility and osteogenic potential of these PLGA/MgPG composite matrices. MgPG matrices demonstrated high cell viability and proliferation of MC3T3-E1 cells as well as increased osteogenic activity reported by alkaline phosphatase activity, calcium deposition and gene expression of Col1a1, osteocalcin, bone sialoprotein and Sp7. Lastly, we investigated the gene expression profile of markers/targets of the canonical β-catenin dependent Wnt signaling pathway with and without inhibitor DKK1 to understand the potential underlying mechanism behind the enhanced osteogenic potential of MgPG. In response to MgPG, gene expression of β-catenin increased, while protein expression of BMP-2 and WISP-1 also increased. These results suggest the influence of MgPG on the Wnt pathway in relation to osteogenic differentiation. With further study, MgPG matrices may provide practical solutions to the problem of effectively regenerating critical-sized bone defects, which remains a challenge in orthopaedics.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf074"},"PeriodicalIF":8.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering stem cell exosomes promotes the survival of multi-territory perforator flap in diabetes via regulating anti-inflammatory and angiogenesis.","authors":"Chao Sun, Junwei Su, Zheng Wang, Changjiang Liu, Xinzeyu Yi, Weimin Chen, Dong Zhang, Aixi Yu","doi":"10.1093/rb/rbaf075","DOIUrl":"10.1093/rb/rbaf075","url":null,"abstract":"<p><p>The versatile multi-territory perforator flap remains a cornerstone of reconstructive surgery for diabetic ulcerations, yet its clinical efficacy faces significant challenges in hyperglycemic conditions. The diabetic milieu significantly exacerbates tissue ischemia through augmented chronic inflammation and impaired angiogenesis, which collectively harm flap perfusion and compromise its overall viability. A major postoperative complication is distal flap necrosis, which is closely associated with the critical \"Choke zone,\" a hypoperfused transitional area that exhibits delayed vascular recruitment and suboptimal angiogenesis. This vascular bottleneck creates a precarious balance between tissue oxygen demand and supply, ultimately compromising flap viability. To address this issue, we have developed the engineering stem cell exosomes by encapsulating metformin-loaded Mesoporous silica nanoparticles into BMSC exosomes (M-MS@EXO NPs), enabling the release of metformin. Compared to traditional oral medication, delivering metformin through engineered exosomes allows for precise administration in diabetic wounds. The multifunctional M-MS@EXO NPs exhibit dual pharmacological activity by reducing the secretion of inflammatory cytokines while effectively remodeling the vascular niche within the diabetic microenvironment. Additionally, the M-MS@EXO NPs show anti-inflammatory and angiogenesis effects by inhibiting TNF/apoptosis and enhancing VEGF signaling pathways in vitro. In the dorsal multi-territory perforator flap model of type 2 diabetes, the M-MS@EXO NPs demonstrate the ability to alleviate inflammation and promote neovascularization of the Choke zone, reducing distal necrosis, which holds great promise for improving flap survival in diabetes.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf075"},"PeriodicalIF":8.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel paclitaxel eluting bioresorbable vascular stent with a super flexible stent structure and round cross section struts fabricated using 3D printing technology with a rotating platform.","authors":"Wei Liu, Qingqing Li, Ge Song, Zhiqi Lin, Xiaofei Gong, Hanqing Feng, Hugh Q Zhao, Yujie Zhou, Yunbing Wang, Zhongyong Fan, Qing Liu","doi":"10.1093/rb/rbaf073","DOIUrl":"10.1093/rb/rbaf073","url":null,"abstract":"<p><p>Bioresorbable stents (BRS) have emerged as a groundbreaking development in the field of percutaneous coronary intervention (PCI) as they address the long-standing concerns of metallic stents. Nevertheless, the observed higher thrombosis rates in the first generation BRS, i.e. ABSORB^®, might be attributed to their thicker struts, slower degradation rate and structural dismantling of partially endothelialized stents. In this study, measures have been taken to overcome these limitations include reducing strut thickness, modifying the structural design to maintain radial strength with thinner round cross section struts and using a new material poly(L-lactide-co-ɛ-caprolactone) (PLCL 95/5) that is tougher and degrade faster than poly(L-lactic acid) (PLLA).Given the excellent biocompatibility of PLCL materials, the US FDA has approved their use in clinical applications. PLCL stents can be used to treat diseases such as tracheal stenosis and tracheoesophageal fistula, and can also be applied in the construction of other tissue engineering stents, such as nerve conduitsand fat filling stents. The newly designed coronary stents were fabricated using a 3D printing technology with a rotating platform, coated with a paclitaxel coating and comprehensive in vitro research was conducted. It was the first to undergo tests in animals. Results showed the novel paclitaxel eluting PLCL stents had super-flexible structure, thinner round cross-sectional struts, a faster degradation profile and satisfactory hemocompatibility. With a paclitaxel dose of 0.57 μg/mm², the drug eluting stents showed very low degree of stenosis within 6 months of implantation in a porcine model. Overall, the results showed that the novel 3D printed PLCL drug eluting stent is a very promising candidate for next generation bioresorbable coronary stent.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf073"},"PeriodicalIF":8.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12371334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible exosomes derived from <i>Pinctada martensii</i> mucus for therapeutic melanin regulation via α-MSH/NF-κB/MITF pathway.","authors":"Dandan Mo, Weihao Zheng, Zixin Gao, Ke Ma, Ke Yang, Tao Zeng, Chaozheng Qin, Yan Luo, Li Zheng, Sheng Xu","doi":"10.1093/rb/rbaf072","DOIUrl":"10.1093/rb/rbaf072","url":null,"abstract":"<p><p>Abnormal melanin production can lead to various pigmentary disorders, which significantly affect patients' quality of life and overall health. However, current clinical melanogenesis inhibitors have adverse side effects such as skin dryness, itching, erythema, etc. In this study, we used naturally isolated exosomes derived from <i>Pinctada martensii</i> mucus (PMMEXOs) and investigated the effects on melanin synthesis based on B16-F10 melanoma cells and zebrafish. We demonstrated that PMMEXOs effectively inhibited melanin production while exhibiting excellent biocompatibility. To elucidate the underlying mechanisms, RNA sequencing and bioinformatics analysis were employed, identifying 556 differentially expressed genes associated with PMMEXOs treatment. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the involvement of the NF-κB signaling pathway in the regulation of melanogenesis. Further mechanistic studies confirmed that PMMEXOs significantly reduced tyrosinase activity and melanin content, accompanied by the downregulation of critical melanogenesis-related genes and proteins, including MITF, TYR, TYRP-1 and TRP-2. Notably, the anti-melanogenic effects of PMMEXOs were mediated by activation of the NF-κB signaling pathway, underscoring their regulatory role in melanin biosynthesis. Additionally, microRNA (miRNA) sequencing of PMMEXOs identified specific miRNAs implicated in immune regulation and modulation of the NF-κB pathway, further supporting their mechanistic involvement in melanin inhibition. These findings collectively position PMMEXOs as a promising and innovative therapeutic strategy for the prevention and treatment of pigmentary disorders such as melasma, age spots and wrinkles.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf072"},"PeriodicalIF":8.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic incorporation of metal ions in bone regenerative scaffolds: multifunctional platforms for advancing osteogenesis.","authors":"Yunnong Luo, Han Zhang, Zhonghan Wang, Jianhang Jiao, Yang Wang, Weibo Jiang, Tong Yu, He Liu, Lili Guan, Mufeng Li, Minfei Wu","doi":"10.1093/rb/rbaf068","DOIUrl":"10.1093/rb/rbaf068","url":null,"abstract":"<p><p>Bone serves as a critical structural framework, enabling movement and protecting internal organs. Consequently, maintaining skeletal health is a pivotal objective in bone tissue engineering. Bioactive metal ions, such as magnesium, strontium, zinc and copper, play essential roles in bone metabolism by participating in key physiological processes that sustain bone health and support regeneration. Recent studies indicate that these ions enhance the physicochemical properties and biological performance of bone tissue engineering materials, thereby facilitating osseointegration through diverse mechanisms. Specifically, magnesium promotes osteogenic differentiation; strontium inhibits osteoclast activity; zinc exhibits antibacterial properties; and copper facilitates vascularization for osteogenesis. Therefore, incorporating bioactive metal ions has emerged as a prevalent strategy in bone tissue engineering to address orthopedic disorders. This review systematically summarizes the roles of magnesium, strontium, zinc and copper in bone repair and regeneration. It provides an in-depth analysis of engineered materials incorporating these ions, with a focus on their applications and modifications across various material types. Furthermore, we explore the synergistic effects of combining these metal ions in bone tissue engineering, emphasizing their enhanced biological properties. By synthesizing recent research findings, this review aims to provide new insights and potential breakthroughs in leveraging bioactive metal ions for advancing treatments of orthopedic diseases.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf068"},"PeriodicalIF":8.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional dynamic cerium-polypeptide hydrogel with antibacterial antioxidative anti-inflammatory for multidrug-resistant bacterial infected wound healing.","authors":"Meng Luo, Jing Tian, Chenxi Xie, Yanzi Zhao, Bo Lei","doi":"10.1093/rb/rbaf071","DOIUrl":"10.1093/rb/rbaf071","url":null,"abstract":"<p><p>Bacterial infection, especially multidrug-resistant (MDR) bacterial infection, is a great challenge in clinical wound repair, highlighting the urgent to develop antibacterial hydrogel dressing. In this work, a multifunctional cerium-polypeptide hydrogel (FEPC) with comprehensive antibacterial, antioxidant, anti-inflammatory and angiogenesis ability was developed for the treatment of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infected wounds. The FEPC hydrogel was constructed using Ce³⁺ and antibacterial polypeptide co-crosslinked γ-polyglutamic acid (γ-PGA) through double dynamic electrostatic and coordination interaction. This unique dynamic architecture endowed FEPC with outstanding injectability and rapid self-healing capacity, ensuring conformal coverage of irregular wounds. <i>In vitro</i> experiments demonstrated that FEPC showed robust antibacterial activity, and could effectively eliminate reactive oxygen species (fixed terminology). Meanwhile, the FEPC hydrogel could downregulate the expression of pro-inflammatory cytokines and promote angiogenesis by upregulating the VEGF expression. Importantly, <i>in vivo</i> assessments using MRSA-infected full-thickness wounds showed that FEPC hydrogel could rapidly repair the MRSA infected wounds (54% wound repair rate on Day 3 for FEPC <i>vs</i> reported hydrogel below 40% rate) and promote epithelialization and hair follicle regeneration within 14 days. Histological analysis confirmed that FEPC hydrogel could significantly inhibit infection and excessive inflammation, as well as accelerate angiogenesis. This work suggests that cerium-polypeptide hydrogel is a perfect partner for treating MDR bacterial infected wounds, providing a viable solution for synergistically combat infection, oxidative stress and other related-disease treatments.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf071"},"PeriodicalIF":8.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12313022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}