Recombinant human collagen type III microgel: an advanced injectable dermal filler for rejuvenating aging skin.

IF 8.1 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2025-07-28 eCollection Date: 2025-01-01 DOI:10.1093/rb/rbaf076
Yafang Chen, Yihan Zhao, Xinyue Zhang, Yang Sun, Kang Li, Liguo Zhang, Shuang Li, Jie Liang, Kefeng Wang, Yujiang Fan
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Abstract

Skin aging, characterized by reduced collagen regeneration, chronic inflammation and heightened skin cancer risk, poses a significant challenge. Collagen-based materials, employed as dermal fillers to smooth wrinkles, have attained extensive utilization. Nevertheless, traditional animal-derived collagen protein primarily presents concerns pertaining to disease risks, potential immunological reactions, and batch instability. Here, we introduced a highly durable 1,4-butanediol diglycidyl ether cross-linked recombinant human collagen type III (rhCol III) microgel as dermal filler for rejuvenating aging skin. The rhCol III microgel exhibited exceptional thermostability, mechanical strength and injectability. Subsequently, we established a UV-photoaging skin animal model and chose rhCol III microgel as a bioactive material for in vivo implantation, systematically comparing its biological effect with commercialized collagen I (Col I) derived from porcine skin (pCollagen) and hyaluronic acid through histological observation, immunofluorescence staining, hydroxyproline quantification and analysis of specific gene expression. Outcomes indicated rhCol III microgel prompted augmented production of Col I, collagen III (Col III) and elastic fibers, thereby contributing to the remodeling of the extracellular matrix (ECM). In summary, our investigation contributed to robust biosafety and rejuvenation of UV-induced skin photoaging by rhCol III under a single injection for 6 weeks. Despite the imperative ongoing efforts required for the successful translation from bench to clinic, the discernibly superior safety and efficacy of rhCol III microgel present an innovative methodology in combating skin aging, offering significant promise in medical cosmetology and tissue engineering.

重组人胶原蛋白III型微凝胶:一种先进的可注射真皮填充剂,使老化的皮肤恢复活力。
皮肤老化,以胶原蛋白再生减少、慢性炎症和皮肤癌风险增加为特征,构成了重大挑战。胶原蛋白为基础的材料,作为真皮填充物,以平滑皱纹,已获得广泛的应用。然而,传统的动物源性胶原蛋白主要存在与疾病风险、潜在免疫反应和批次不稳定性有关的问题。在这里,我们介绍了一种高度耐用的1,4-丁二醇二缩水甘油醚交联重组人胶原蛋白III型(rhCol III)微凝胶作为真皮填充剂,使老化的皮肤恢复活力。rhCol III微凝胶具有优异的热稳定性、机械强度和注射性。随后,我们建立了紫外线光老化皮肤动物模型,选择rhCol III微凝胶作为生物活性材料进行体内植入,通过组织学观察、免疫荧光染色、羟脯氨酸定量和特异性基因表达分析,系统地比较了rhCol III微凝胶与商业化的猪皮肤I型胶原蛋白(Col I)和透明质酸的生物学效果。结果表明,rhCol III微凝胶促进Col I、胶原III (Col III)和弹性纤维的生成,从而促进细胞外基质(ECM)的重塑。总之,我们的研究表明,单次注射rhCol III对紫外线诱导的皮肤光老化具有强大的生物安全性和再生能力。尽管从实验室到临床的成功转化需要不断的努力,但rhCol III微凝胶具有明显的安全性和有效性,为对抗皮肤衰老提供了一种创新的方法,在医学美容和组织工程方面提供了重大的希望。
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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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