Regenerative Biomaterials最新文献

Retraction of: Material and regenerative properties of an osteon-mimetic cortical bone-like scaffold. 仿骨皮质骨样支架的材料和再生特性。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-04-29 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag067

引用次数: 0

Polydopamine-mediated EGCG-modified polystyrene microspheres for the synergistic removal of inflammatory cytokines TNF-α and bilirubin in liver failure. 聚多巴胺介导的egcg修饰聚苯乙烯微球协同去除肝衰竭中炎症细胞因子TNF-α和胆红素。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-04-10 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag069

Yunzheng Du, Zhuang Liu, Yamin Chai, Biao Wang, Lichun Wang, Zimeng Wang, Xiaofang Guo, Guanlun Zhou, Jingxuan Yang, Chunling Zhu, Leilei Yang, Xinyao Lv, Lailiang Ou

{"title":"Polydopamine-mediated EGCG-modified polystyrene microspheres for the synergistic removal of inflammatory cytokines TNF-α and bilirubin in liver failure.","authors":"Yunzheng Du, Zhuang Liu, Yamin Chai, Biao Wang, Lichun Wang, Zimeng Wang, Xiaofang Guo, Guanlun Zhou, Jingxuan Yang, Chunling Zhu, Leilei Yang, Xinyao Lv, Lailiang Ou","doi":"10.1093/rb/rbag069","DOIUrl":"https://doi.org/10.1093/rb/rbag069","url":null,"abstract":"Hemoperfusion has emerged as a crucial treatment for liver failure in clinics. However, the challenge remains in the incapacity to effectively and concurrently eliminate multiple harmful toxins, including inflammatory cytokines and bilirubin. This study employed molecular docking for directing the design of adsorbents. To develop a multi-target adsorbent material for liver failure, capable of absorbing both bilirubin and tumor necrosis factor-α (TNF-α), a functional platform was constructed. This platform employs a TiO2-modified polystyrene-based microsphere PSVT, which was designed for bilirubin adsorption, and coated with polydopamine and grafted with epigallocatechin gallate (EGCG) for TNF-α adsorption. The results confirm that EGCG possesses a high binding affinity for TNF-α, and the introduction of EGCG as a functional moiety markedly improves the clearance effectiveness of TNF-α. Meanwhile, PSVT served as the foundation to guarantee the effective removal of bilirubin. PSVT/P/EGCG exhibits outstanding clearance performance, with a maximum adsorption capacity of 263 ng/g for TNF-α and 23.91 mg/g for bilirubin, demonstrating its high efficiency in removing both inflammatory mediators and toxins. EGCG contributes exceptional antioxidant capabilities to PSVT/P/EGCG. The adsorbent exhibits remarkable biocompatibility and stability. The multi-target adsorbent material developed in this study has an extensive spectrum of potential applications in liver failure treatment employing an artificial liver.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag069"},"PeriodicalIF":8.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-scavenging lipoic acid-modified chitosan hydrogels with rapid photocrosslinked ability accelerates peripheral nerve regeneration. 清除ros的硫辛酸修饰壳聚糖水凝胶具有快速光交联能力，可促进周围神经再生。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-04-03 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag068

Xianglong Chen, Ren Gao, Xinyue Liang, Kun Liu, Zhong Wei, Xiaopei Wu, Takashi Goto, Chengjie Xiong, Feng Xu, Honglian Dai

{"title":"ROS-scavenging lipoic acid-modified chitosan hydrogels with rapid photocrosslinked ability accelerates peripheral nerve regeneration.","authors":"Xianglong Chen, Ren Gao, Xinyue Liang, Kun Liu, Zhong Wei, Xiaopei Wu, Takashi Goto, Chengjie Xiong, Feng Xu, Honglian Dai","doi":"10.1093/rb/rbag068","DOIUrl":"https://doi.org/10.1093/rb/rbag068","url":null,"abstract":"Peripheral nerve injury (PNI) triggers excessive oxidative stress and inflammation that impede nerve regeneration, leading to target organ atrophy and incomplete functional recovery. Conventional drug-loaded nerve conduits release anti-inflammatory drugs sustainably via filled hydrogels, requiring a precisely engineered hydrogel system to match drug release kinetics without impeding regenerating nerve tissue growth. Meanwhile, constructing complex conduit systems may introduce potential cytotoxic factors and induce aseptic inflammation via acidic degradation products. Herein, we fabricated a ROS-scavenging CS-LA/P(MMD-CL) composite nerve conduit with rapid photo-crosslinking (365 nm UV irradiation, photoinitiator-free) via integrating lipoic acid-modified chitosan (CS-LA) hydrogel into an oriented P(MMD-CL) conduit. Disulfide bonds in CS-LA scavenged ROS to alleviate oxidative stress, modulate inflammatory factor expression and resolve excessive inflammation, while the oriented P(MMD-CL) provided physical support and directional guidance for axon growth. In vivo studies on SD rats revealed that CS-LA/P(MMD-CL) promoted nerve regeneration through enhancing cell proliferation and angiogenesis, improving the maturity of regenerative peripheral nerve, mitigating gastrocnemius atrophy and promoting nerve function recovery (SFI: -77.51 ± 1.51), and exhibited great biosafety-no organ damage from degradation byproducts. This simple, multifunctional composite conduit effectively modulates the PNI microenvironment and promotes peripheral nerve regeneration, offering a promising strategy for PNI repair.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag068"},"PeriodicalIF":8.1,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional-printed ROS-scavenging and immunomodulatory hydrogel accelerates diabetic wound healing through synergistic microenvironment regulation. 三维打印ros清除和免疫调节水凝胶通过协同微环境调节加速糖尿病伤口愈合。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-28 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag066

Xin Cao, Yang Wang, Yuanhang Xu, Nai Liang, Jiabao Xu, Xinyun Li, Cong Ye, Caichou Zhao

{"title":"Three-dimensional-printed ROS-scavenging and immunomodulatory hydrogel accelerates diabetic wound healing through synergistic microenvironment regulation.","authors":"Xin Cao, Yang Wang, Yuanhang Xu, Nai Liang, Jiabao Xu, Xinyun Li, Cong Ye, Caichou Zhao","doi":"10.1093/rb/rbag066","DOIUrl":"https://doi.org/10.1093/rb/rbag066","url":null,"abstract":"The management of diabetic wounds remains clinically challenging, primarily owing to three interrelated pathological mechanisms: excessive accumulation of reactive oxygen species (ROS), dysregulated angiogenesis and sustained, non-resolving inflammation. To address these issues, we developed a multifunctional 3D-printed nanoparticle enhanced bionic skin (3D-NEBS) by integrating polydopamine-gallium-arginine nanoparticles (PDA-Ga-Arg) into a self-healing hydrogel matrix composed of oxidized Tremella fuciformis polysaccharide, carboxymethyl chitosan and fish gelatin. The resulting dressing exhibited mechanical adaptability, pH-responsive release, robust ROS-scavenging capacity and targeted immunomodulatory activity. In vitro, 3D-NEBS promoted M2 macrophage polarization, reduced inflammation, enhanced nitric oxide (NO) production and improved endothelial cell migration and tube formation. Broad-spectrum efficacy was confirmed, with significant growth inhibition observed against S. aureus and E. coli. In a diabetic rat model, 3D-NEBS achieved 92.79% ± 0.39% wound closure within 14 days, with enhanced collagen deposition and neovascularization. Transcriptomic analysis revealed upregulation of pathways related to collagen synthesis, antioxidant response and immune regulation. This study presents a synergistic hydrogel-nanoparticle platform with strong potential for diabetic wound repair.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag066"},"PeriodicalIF":8.1,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Betaine based organ preservation solution alleviates renal allograft I/R injury by protecting endothelial cells. 甜菜碱器官保存液通过保护内皮细胞减轻同种异体肾移植I/R损伤。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-26 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag064

Mingxing Yu, Shuo Li, Xu Duan, Yiran Wang, Tao Ming, Shaohua Wu, Xihe Wang, Zhongyang Shen, Xunfeng Zou, Deling Kong, Tingting Lan

引用次数: 0

Synergistic piezo-immunotherapy enabled by lithium-doped SrTiO₃ nanocatalysts for potent tumor ablation through ROS generation and immune activation. 锂掺杂SrTiO3纳米催化剂的协同压电免疫治疗通过ROS生成和免疫激活来有效消融肿瘤。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-24 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag030

Yuzheng Gao, Yichun Zhang, Shuyan Zhang, Zhiwei Yang, Wenjing Liu, Jing Zhang, Zhipeng Gu, Xianchun Chen

{"title":"Synergistic piezo-immunotherapy enabled by lithium-doped SrTiO3 nanocatalysts for potent tumor ablation through ROS generation and immune activation.","authors":"Yuzheng Gao, Yichun Zhang, Shuyan Zhang, Zhiwei Yang, Wenjing Liu, Jing Zhang, Zhipeng Gu, Xianchun Chen","doi":"10.1093/rb/rbag030","DOIUrl":"https://doi.org/10.1093/rb/rbag030","url":null,"abstract":"Piezocatalytic tumor therapy represents an emerging approach in cancer treatment, leveraging sonosensitizers to generate reactive oxygen species (ROS) under ultrasound (US) irradiation for effective tumor eradication. However, enhancing ROS production efficiency remains a critical challenge in this field. In this study, SrTiO3 (STO) was selected as the base piezocatalytic material, and its performance was optimized through a combined strategy of lithium doping and oxygen vacancy engineering. The modified material (designated 1.5LSTO) exhibits substantially enhanced local electrical responses. As quantified by PFM, its surface potential and piezoelectric (butterfly-type) amplitude were ∼2.23-fold higher than those of the unmodified sample. The optimally modified material, designated as 1.5LSTO, exhibited a 1.44-fold enhancement in piezocatalytic activity compared to pristine STO under US exposure, enabling efficient generation of hydroxyl radicals (•OH) and superoxide anions (• <math> <mrow> <msubsup><mrow><mi>O</mi></mrow> <mrow><mn>2</mn></mrow> <mrow><mo> </mo> <mi>-</mi></mrow> </msubsup> </mrow> </math> ). In vitro experiments demonstrated significant cytotoxicity of 1.5LSTO against tumor cells. Furthermore, in vivo studies using an intestinal tumor-bearing mouse model confirmed that US-activated 1.5LSTO effectively suppressed tumor proliferation and promoted apoptosis. Notably, lithium doping was found to significantly upregulate CD8+ T cell expression, indicating an immunomodulatory effect. The integration of piezocatalysis with immune activation resulted in a multimodal synergistic therapy that substantially improved overall antitumor efficacy. This work provides an innovative material-based strategy for enhancing tumor treatment through functional modulation and synergistic mechanisms.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag030"},"PeriodicalIF":8.1,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13075956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic integration of biomaterials in orthopedic implantation for infection preventing and tissue engineering. 生物材料在骨科植入术中协同整合预防感染和组织工程。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-24 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag061

Longhui Xu, Xu Chen, Zeyang Cao, Guoshuang Zheng, Zhengyong Li, Xiao Yang, Hui Xie, Siyu Chen

{"title":"Synergistic integration of biomaterials in orthopedic implantation for infection preventing and tissue engineering.","authors":"Longhui Xu, Xu Chen, Zeyang Cao, Guoshuang Zheng, Zhengyong Li, Xiao Yang, Hui Xie, Siyu Chen","doi":"10.1093/rb/rbag061","DOIUrl":"https://doi.org/10.1093/rb/rbag061","url":null,"abstract":"The treatment of infected bone defects (IBD) is challenging in orthopedics because of their complex pathological mechanisms and high recurrence rates. An ideal therapeutic strategy should simultaneously eradicate infection and promote bone regeneration. However, conventional approaches rarely achieve both objectives effectively. It has concurrently high recurrence rates, prolonged treatment courses and limited therapeutic efficacy. Clinically, implant-associated infections leading to IBD are often exacerbated by patients'underlying diseases, material-related risks and perioperative factors, thereby necessitating multifunctional strategies to achieve an integrated solution. With the rapid development of bone graft materials, researchers have focused on biocomposites because of their unique multifunctionality. By integrating the advantages of different materials and incorporating various bioactive components, biocomposites allow comprehensive treatment of patients with IBD. In this article, we reviewed the advancements in the application of different types of biocomposites in treating IBD, highlighting their synergistic effects in promoting bone healing and combating infections through various mechanisms. Additionally, it addresses the limitations associated with clinical translation and proposes a regulatory science-based framework for full lifecycle assessment. This framework offers a standardized evaluation system and precise regulatory strategies for personalized biocomposites with multiple functions. These insights provide scientific support and reveal novel approaches for clinical applications.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag061"},"PeriodicalIF":8.1,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13099654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-functional DHBA/Zn-hybrid electrospun scaffolds for simultaneous nerve repair and bone regeneration. 双功能DHBA/ zn杂化电纺丝支架同时修复神经和骨再生。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-21 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag062

Yiding Shen, Yilong Dong, Peng Hua, Hongyu Luo, Xinkun Shen, Kai Fang, Lingzhuo Hou, Yeyi Zheng, Gaowen Li, Litai Jin, Yongping Yuan, Pingping Ma

{"title":"Dual-functional DHBA/Zn-hybrid electrospun scaffolds for simultaneous nerve repair and bone regeneration.","authors":"Yiding Shen, Yilong Dong, Peng Hua, Hongyu Luo, Xinkun Shen, Kai Fang, Lingzhuo Hou, Yeyi Zheng, Gaowen Li, Litai Jin, Yongping Yuan, Pingping Ma","doi":"10.1093/rb/rbag062","DOIUrl":"https://doi.org/10.1093/rb/rbag062","url":null,"abstract":"Oral and maxillofacial surgeries or trauma (e.g. impacted tooth extraction, jaw fracture or tumor resection) often lead to concurrent peripheral nerve injury and bone defects, while current collagen/gelatin sponges offer limited therapeutic effects. To address this challenge, we developed innovative electrospun scaffolds (MOF2, MOF4 and MOF6) by in situ synthesis of 3,5-dihydroxybenzoic acid/zinc (DHBA/Zn-MOF) hybrids within a gelatin/polycaprolactone matrix. In vitro, Schwann cells treated with material extracts exhibited enhanced migration, regulated myelin-associated genes (Ngf/Pmp22 upregulated, Ncam downregulated) and increased NGF protein expression via the PI3K pathway. Co-cultured PC12 cells showed increased neurite outgrowth, confirming neural repair potential. Osteoblasts exposed to material extracts showed elevated alkaline phosphatase activity, enhanced mineralization and upregulated osteogenic genes (Runx2, Alp and Opg), verifying osteogenic capacity. In vivo, MOF6 scaffolds achieved superior motor function recovery in a rat sciatic nerve crush model (evidenced by increased compound muscle action potentials and reduced gastrocnemius muscle atrophy) and promoted trabecular bone formation in a rat skull defect model (validated by micro-CT and histological analyses). These findings underscore the dual-functional capability of DHBA/Zn-hybrid scaffolds to simultaneously promote nerve repair and bone regeneration, offering a promising therapeutic approach for complex neuro-bone composite injuries in clinical practice.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag062"},"PeriodicalIF":8.1,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13105838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-peptide engineered macrophage membrane biomimetic nanosystem via targeting Rg1 delivery for traumatic brain injury therapy. 靶向Rg1递送的双肽工程巨噬细胞膜仿生纳米系统用于创伤性脑损伤治疗。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-20 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag059

Weiquan Liao, Zhichao Lu, Ziheng Li, Chenxing Wang, Xingjia Zhu, Jue Zhu, Yongqi Zhu, Jialiang Lin, Jiajia Wen, Xuanfeng Chen, Jian Chen, Jianhong Shen, Youlang Zhou, Peipei Gong

{"title":"Dual-peptide engineered macrophage membrane biomimetic nanosystem via targeting Rg1 delivery for traumatic brain injury therapy.","authors":"Weiquan Liao, Zhichao Lu, Ziheng Li, Chenxing Wang, Xingjia Zhu, Jue Zhu, Yongqi Zhu, Jialiang Lin, Jiajia Wen, Xuanfeng Chen, Jian Chen, Jianhong Shen, Youlang Zhou, Peipei Gong","doi":"10.1093/rb/rbag059","DOIUrl":"https://doi.org/10.1093/rb/rbag059","url":null,"abstract":"Traumatic brain injury (TBI) induces a detrimental inflammatory microenvironment at the lesion site, which, together with neuronal death and loss, leads to neurological dysfunction. The blood-brain barrier (BBB) further impedes intracerebral drug delivery, posing a major challenge for post-TBI therapy. To overcome this, we developed a brain-targeted biomimetic nanosystem (R/T-MaM-NPs) using an engineered dual-peptide-modified macrophage membrane (MaM). This system encapsulates neuroprotective ginsenoside Rg1 into poly (lactic-co-glycolic acid)-based nanoparticles (NPs). RAW264.7 macrophages were engineered to co-express targeting peptides (RVG and T7) on their membranes; the derived R/T-MaM was then coated onto NPs. The MaM coating conferred high biocompatibility and biosafety, enabling R/T-MaM-NPs to reduce immune clearance and prolong systemic circulation. By leveraging the intrinsic inflammatory chemotaxis of MaM and dual-peptide targeting, the integrated system promoted traversal across the BBB and subsequent accumulation around the cerebral lesion, thereby inducing the transdifferentiation of reactive astrocytes (RAs) into electrophysiologically functional neuron-like cells. RNA sequencing confirmed significant upregulation of neurogenic genes in R/T-MaM-NP-treated RAs, an outcome closely linked to suppression of the Wnt/Notch signaling pathway. Furthermore, R/T-MaM-NPs remodeled the inflammatory microenvironment at the TBI site, alleviated cerebral edema, and enhanced the recovery of cognitive and motor functions in TBI mice.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag059"},"PeriodicalIF":8.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13106896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel dual-targeting strategy to suppress cariogenic bacteria and biofilms with engineered ARG/FBG bioactive composites. ARG/FBG生物活性复合材料抑制龋齿细菌和生物膜的新型双靶向策略。

IF 8.1 1区医学

Regenerative Biomaterials Pub Date : 2026-03-20 eCollection Date: 2026-01-01 DOI: 10.1093/rb/rbag058

Xili Qiu, Tongjun Li, Jincai Guo, Hua Liu, Jing Liu, Xiaojing Chen

{"title":"A novel dual-targeting strategy to suppress cariogenic bacteria and biofilms with engineered ARG/FBG bioactive composites.","authors":"Xili Qiu, Tongjun Li, Jincai Guo, Hua Liu, Jing Liu, Xiaojing Chen","doi":"10.1093/rb/rbag058","DOIUrl":"10.1093/rb/rbag058","url":null,"abstract":"Dental caries is a biofilm-mediated disease primarily driven by Streptococcus mutans (S. mutans), which ferments dietary sugars to produce acids that demineralize enamel. This study developed a novel composite combining L-arginine (ARG) and fluoride-containing bioactive glass (FBG) to achieve dual antibacterial and antibiofilm effects. The ARG/FBG composite (1:1 mass ratio) was assessed in vitro against S. mutans. Antibacterial activity was quantified by AlamarBlue assay and colony-forming unit (CFU) counts, while antibiofilm efficacy was evaluated using scanning electron microscopy and confocal laser scanning microscopy. ARG inhibited planktonic growth but showed limited biofilm disruption, whereas FBG effectively disrupted biofilm architecture without affecting planktonic growth. The composite (7.5 mg/mL ARG + 7.5 mg/mL FBG) demonstrated enhanced complementary action, achieving near-complete eradication of planktonic cells (∼6-log10 CFU reduction at 24 h) and profound biofilm destruction. TEM-EDS revealed intracellular co-localization of fluoride, calcium and phosphorus within treated bacteria. RNA sequencing and RT-qPCR showed downregulated key virulence genes (ldh and luxS) and disrupted metabolic pathways related to carbon utilization and acid production. The composites exhibited favorable biocompatibility. These findings indicate that ARG/FBG bioactive composites potently suppress S. mutans through biofilm disruption, metabolic inhibition and potential in situ mineralization, offering a promising strategy for caries prevention.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"13 ","pages":"rbag058"},"PeriodicalIF":8.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0