磷酸镁功能化氧化石墨烯和PLGA复合基质,增强了骨再生的机械和成骨性能。

IF 8.1 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2025-07-26 eCollection Date: 2025-01-01 DOI:10.1093/rb/rbaf074
Taraje Whitfield, Fatemeh S Hosseini, Jason D Orlando, Chenyun Deng, Kevin W-H Lo, Ho-Man Kan, Debolina Ghosh, Stefanie A Sydlik, Cato T Laurencin
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引用次数: 0

摘要

骨缺陷每年影响数百万人,使骨组织成为开发治疗方法的特别兴趣。目前的治疗策略存在缺陷。再生工程试图通过利用合成骨移植物来实现有效的骨再生,以避免这些缺点。一种提供这种好处的材料是一类功能性石墨材料,称为磷酸石墨烯。虽然我们的许多研究都集中在磷酸钙石墨烯上,但镁也具有成骨作用。因此,在本研究中,我们利用再生工程技术将磷酸镁石墨烯(MgPG)掺入聚乳酸-羟基乙酸(PLGA)中,以制造复合微球基基质作为潜在的人工骨移植物。在PLGA基质中加入不同量的MgPG,研究了MgPG对PLGA基质形态、结构、物理和生物学特性的影响。含mgpg的基质表现出良好的机械强度、亲水性和可降解性,而不影响基质的形态。由于MgPG是一种氧化石墨烯衍生物,镁和磷酸盐离子能够作为诱导剂支持骨愈合,因此我们接下来评估了这些PLGA/MgPG复合基质的细胞相容性和成骨潜力。碱性磷酸酶活性、钙沉积、Col1a1、骨钙素、骨涎蛋白和Sp7基因表达均表明MgPG基质具有较高的细胞活力和MC3T3-E1细胞的增殖能力,并增强了成骨活性。最后,我们研究了经典β-catenin依赖性Wnt信号通路的标志物/靶点的基因表达谱,以了解MgPG增强成骨潜能背后的潜在潜在机制。MgPG作用后,β-catenin基因表达增加,BMP-2和WISP-1蛋白表达也增加。这些结果表明MgPG对Wnt通路的影响与成骨分化有关。随着进一步的研究,MgPG基质可能为有效再生临界尺寸骨缺损提供实用的解决方案,这仍然是骨科的一个挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Magnesium phosphate functionalized graphene oxide and PLGA composite matrices with enhanced mechanical and osteogenic properties for bone regeneration.

Bone defects affect millions of people annually, making bone tissue of particular interest for developing treatments. Current strategies for healing suffer drawbacks. Regenerative engineering seeks to achieve efficient bone regeneration by utilizing synthetic bone grafts to evade these drawbacks. One material that offers such benefits is a class of functional graphenic material, known as Phosphate Graphenes. While many of our studies have focused on Calcium Phosphate Graphene, magnesium is also osteogenic. Therefore, in this study, we utilized regenerative engineering techniques to incorporate Magnesium Phosphate Graphene (MgPG) into poly(lactic-co-glycolic acid) (PLGA) to fabricate composite microsphere-based matrices as a potential synthetic bone graft. Employing different amounts of MgPG within PLGA matrices, we studied the effect of MgPG on the morphological, structural, physical and biological characteristics. MgPG-containing matrices demonstrated great mechanical strength, hydrophilicity and degradability without compromising matrix morphology. Because MgPG is a graphene oxide derivative with magnesium and phosphate ions capable of supporting bone healing as inducerons, we next evaluated the cytocompatibility and osteogenic potential of these PLGA/MgPG composite matrices. MgPG matrices demonstrated high cell viability and proliferation of MC3T3-E1 cells as well as increased osteogenic activity reported by alkaline phosphatase activity, calcium deposition and gene expression of Col1a1, osteocalcin, bone sialoprotein and Sp7. Lastly, we investigated the gene expression profile of markers/targets of the canonical β-catenin dependent Wnt signaling pathway with and without inhibitor DKK1 to understand the potential underlying mechanism behind the enhanced osteogenic potential of MgPG. In response to MgPG, gene expression of β-catenin increased, while protein expression of BMP-2 and WISP-1 also increased. These results suggest the influence of MgPG on the Wnt pathway in relation to osteogenic differentiation. With further study, MgPG matrices may provide practical solutions to the problem of effectively regenerating critical-sized bone defects, which remains a challenge in orthopaedics.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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