Regenerative Biomaterials最新文献

{"title":"Smart materials strategy for vascular challenges targeting in-stent restenosis: a critical review.","authors":"Kai Zhang, Wenzhao Liang, Xiao-Bo Chen, Jing Mang","doi":"10.1093/rb/rbaf020","DOIUrl":"https://doi.org/10.1093/rb/rbaf020","url":null,"abstract":"<p><p>In-stent restenosis (ISR) presents a major challenge in vascular disease management, often leading to complications and repeated interventions. This review article explores the potential of existing smart materials strategies in addressing ISR, emphasizing advancements in materials science and biomedical engineering. We focus on innovative solutions such as bioactive coatings and responsive polymers that offer targeted responses to ISR-related internal and external triggers. These smart materials can dynamically adapt to the physiological conditions within blood vessels, responding in real time to various stimuli such as pH, oxidative stress and temperature. Moreover, we discuss preclinical progress and translational challenges associated with these materials as they move toward clinical applications. The review highlights the importance of controlled drug release and the need for materials that can degrade appropriately to minimize adverse effects. This work aims to identify critical research gaps and provide guidance to encourage interdisciplinary efforts to advance the development of smart stent technologies. Ultimately, the goal is to improve patient outcomes in vascular interventions by leveraging the capabilities of intelligent biomaterials to enhance ISR management and ensure better long-term efficacy and safety in-stent applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf020"},"PeriodicalIF":5.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical methods in studying cell force sensing: principles, current technologies and perspectives.","authors":"Xiaojun Liu, Lei Yu, Adam Xiao, Wenxu Sun, Han Wang, Xiangxiu Wang, Yanghao Zhou, Chao Li, Jiangtao Li, Yongliang Wang, Guixue Wang","doi":"10.1093/rb/rbaf007","DOIUrl":"https://doi.org/10.1093/rb/rbaf007","url":null,"abstract":"<p><p>Mechanical stimulation plays a crucial role in numerous biological activities, including tissue development, regeneration and remodeling. Understanding how cells respond to their mechanical microenvironment is vital for investigating mechanotransduction with adequate spatial and temporal resolution. Cell force sensing-also known as mechanosensation or mechanotransduction-involves force transmission through the cytoskeleton and mechanochemical signaling. Insights into cell-extracellular matrix interactions and mechanotransduction are particularly relevant for guiding biomaterial design in tissue engineering. To establish a foundation for mechanical biomedicine, this review will provide a comprehensive overview of cell mechanotransduction mechanisms, including the structural components essential for effective mechanical responses, such as cytoskeletal elements, force-sensitive ion channels, membrane receptors and key signaling pathways. It will also discuss the clutch model in force transmission, the role of mechanotransduction in both physiology and pathological contexts, and biomechanics and biomaterial design. Additionally, we outline analytical approaches for characterizing forces at cellular and subcellular levels, discussing the advantages and limitations of each method to aid researchers in selecting appropriate techniques. Finally, we summarize recent advancements in cell force sensing and identify key challenges for future research. Overall, this review should contribute to biomedical engineering by supporting the design of biomaterials that integrate precise mechanical information.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf007"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable polymeric occluder with controllable locking structure for closure of atrial septal defect via interventional treatment.","authors":"Daokun Shi, Yahong Kang, Weijie Wang, Ruili Liu, Quansheng Tang, Zhaomin Li, Hongyan Jiang, Jiandong Ding","doi":"10.1093/rb/rbaf016","DOIUrl":"https://doi.org/10.1093/rb/rbaf016","url":null,"abstract":"<p><p>Atrial septal defect (ASD) is one of the major congenital heart diseases, and transcatheter closure with a cardiac occluder is a modern method to treat ASD with the advantage of mini-invasiveness over traditional surgical closure. While current occlusion devices are mainly made of non-degradable nitinol with superelasticity, the permanent existence of a metal <i>in vivo</i> may trigger potential complications and especially has an adverse effect on the heart development for children. However, it is challenging to invent a superelasticity-free occluder that can be delivered through a catheter but firmly locked after being opened at the target site; it is also much desired for research and development to quickly assess the feasibility of a superelasticity-free occluder <i>in vitro</i>. Herein, a biodegradable poly(L-lactide) (PLLA) occluder composed of a braided PLLA frame as the skeleton and a nonwoven PLLA fabric as the flow-blocking membrane is developed, and a controllable locking structure is designed to enable firm closure for a device even without superelasticity. We also suggest and justify a series of <i>in vitro</i> methods to assess the efficacy of the biodegradable occluder, and the results confirm the reliability of locking, water-blocking, mechanical strength and degradability. It is found that the PLLA fabric with moderate fiber density is optimal for surface endothelialization. We also carry out biological assessments; significant endothelialization and alleviated inflammation response are observed after 6 months of subcutaneous implantation into rabbits. The porcine model illustrates that the biodegradable polymeric occluder can be successfully implanted into the atrial septum via transcatheter intervention; the follow-ups have confirmed the safety and efficacy of this biodegradable polymeric occluder with the controllable locking structure.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf016"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12005900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrahedral framework nucleic acids ameliorate cholestatic liver disease by activating Wnt/β-catenin signaling and promoting ERK1/2 phosphorylation.","authors":"Jiaming Zhou, Chenxi Tang, Xin Song, Yating Wang, Bingru Lin, Mengchi Lin, Zixin Xu, Shihua Lin, Chengfu Xu, Chaohui Yu","doi":"10.1093/rb/rbaf017","DOIUrl":"10.1093/rb/rbaf017","url":null,"abstract":"<p><p>Cholestatic liver disease (CLD) is characterized by disruptions in bile formation, secretion and excretion, leading to progressive liver injury, inflammation and fibrosis. Effective treatments to halt or reverse the progression of CLD remain limited. The Wnt/β-catenin signaling pathway has been implicated in the regulation of bile acid homeostasis and liver regeneration, playing a complex role in CLD pathophysiology. Tetrahedral framework nucleic acids (TFNAs), a class of anti-inflammatory and antioxidant DNA nanomaterials, have shown potential in promoting mammalian cell proliferation through activation of cell cycle and proliferation-related signaling pathways. However, their therapeutic potential in CLD has not been fully explored. In this study, we investigated the effects of TFNAs in an α-naphthyl isothiocyanate (ANIT)-induced mouse model of CLD. TFNAs demonstrated the ability to enter hepatocytes, where they activated the Wnt/β-catenin signaling pathway and enhanced ERK1/2 phosphorylation. These molecular changes resulted in significant improvements in liver injury markers, bile acid metabolism and liver regeneration. Complementary <i>in vitro</i> experiments revealed that TFNAs reduced hepatocyte apoptosis and oxidative stress, while promoting cell viability and proliferation. Histological analysis confirmed that TFNAs treatment mitigated liver necrosis, reduced ductular reactions and decreased neutrophil infiltration, highlighting their anti-inflammatory and tissue-protective effects. These findings provide compelling evidence that TFNAs can ameliorate CLD by modulating key signaling pathways involved in hepatocyte survival, regeneration and bile acid homeostasis. Collectively, our findings highlight the therapeutic potential of TFNAs as a novel treatment for CLD and paves the way for further exploration of nanomaterials in liver disease therapy.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf017"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of protein conformational transition accompanied with crosslinking density cues in silk fibroin hydrogels on the proliferation and chondrogenesis of encapsulated stem cells.","authors":"Guolong Cai, Weikun Zhao, Tianhao Zhu, Ana L Oliveira, Xiang Yao, Yaopeng Zhang","doi":"10.1093/rb/rbaf019","DOIUrl":"https://doi.org/10.1093/rb/rbaf019","url":null,"abstract":"<p><p>Silk fibroin (SF) hydrogels possess excellent biocompatibility and biomimetic properties of the extracellular matrix. Among them, the mild chemical crosslinked SF hydrogels show great application potential in the fields of 3D cell culture and tissue repairing and thus have attracted widespread attention. However, the mobility of hydrophobic chain segments of SF molecules in these chemical crosslinked hydrogels can easily cause the molecules to undergo a self-assembly process from random coil to <i>β</i>-sheet conformation due to its lower energy state, thus inducing an inevitable conformational transition process. This process further leads to dynamic changes of important material features, such as the hydrogel pore size and mechanical properties, which can probably bring some non-negligible and unknown impacts on cell behaviors and their biomedical applications. In this study, a typical mild crosslinking system composed of horseradish peroxidase and hydrogen peroxide was chosen to prepare SF hydrogels. A feasible protein conformational transition rate controlling strategy based on hydrogel crosslinking density regulation was also proposed. Our results demonstrate that the lower the hydrogel crosslinking density, the faster the conformational transition rate. Subsequently, SF hydrogels with different conformational transition rates were successfully constructed to investigate the impact of the protein conformational transition rate accompanied with initial crosslinking density on the proliferation and chondrogenic differentiation of encapsulated stem cells. Results comprehensively illustrated that the conformational transition process could effectively regulate cell behavior. The hydrogel with an appropriate conformational transition rate obviously promoted the proliferation and chondrogenesis of encapsulated stem cells, while too fast or too slow transition processes slowed down these cell activities. These findings are hopefully to provide valuable guidance for the development and efficient usage of SF hydrogels in the fields of 3D cell culture and tissue engineering.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf019"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEG-based polyurethane bioadhesive for wet and adaptable adhesion to circumcision wounds.","authors":"Yaqiang Jiang, Zhaoguo Zhang, Chengkai Xuan, Xuetao Shi","doi":"10.1093/rb/rbaf018","DOIUrl":"10.1093/rb/rbaf018","url":null,"abstract":"<p><p>Effective wound management is critical in post-operative recovery, particularly in sensitive areas such as circumcision. The aim of this study was to design and assess the efficacy of a novel two-component polyurethane (PU) bioadhesive, designated as PU1000S, for its application in advanced wound care within the context of clinical circumcision procedures. The glue-type bioadhesive was fine-tuned to conformally adhere to the moist tissue surfaces. It rapidly absorbed interfacial water and cured within 160 s, ensuring remarkable surface adaptability to wet tissue surfaces. The PU1000S demonstrated superior lap-shear strength, peaking at 55.12 ± 6.88 kPa, along with exceptional durability. These attributes underscored its strong wet adhesion and remarkable resilience at the interface with moist tissues. Owing to its mechanical adaptability to wet skin tissue, the adhesive layer of PU1000S maintained stability under complex loading associated with twisting, folding and significant volumetric deformation, resulting in minimal debonding. In addition, PU1000S was found to significantly accelerate wound healing by promoting re-epithelialization and collagen deposition, confirming its excellent bioadaptability for initial closure and subsequent tissue repair and regeneration following circumcision. The comprehensive results position PU1000S as a promising candidate for advanced wound care in circumcision, offering superior performance in terms of wet adhesion, durability and bioadaptability. Its application could potentially enhance clinical outcomes and elevate patient satisfaction.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf018"},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing β-adrenoreceptor agonism for recovery after volumetric muscle loss through regenerative rehabilitation and biomaterial delivery approaches.","authors":"Jennifer McFaline-Figueroa, Christiana J Raymond-Pope, Joseph J Pearson, Albino G Schifino, Junwon Heo, Thomas J Lillquist, Emma E Pritchard, Elizabeth A Winders, Edward T Hunda, Johnna S Temenoff, Sarah M Greising, Jarrod A Call","doi":"10.1093/rb/rbaf015","DOIUrl":"https://doi.org/10.1093/rb/rbaf015","url":null,"abstract":"<p><p>Volumetric muscle loss (VML) injury results in the unrecoverable loss of muscle mass and contractility. Oral delivery of formoterol, a β₂-adrenergic receptor agonist, produces a modest recovery of muscle mass and contractility in VML-injured mice. The objective of this study was to determine if a regenerative rehabilitation paradigm or a regenerative medicine paradigm could enhance the recovery of VML-injured muscle. Regenerative rehabilitation involved oral formoterol delivery combined with voluntary wheel running. Regenerative medicine involved direct delivery of formoterol to VML-injured muscle using a non-biodegradable poly(ethylene glycol) biomaterial. To determine if the regenerative rehabilitation or regenerative medicine approaches were effective at 8 weeks post-injury, muscle mass, contractile function, metabolic function, and histological evaluations were used. One model of regenerative rehabilitation, in which rehabilitation was delayed until 1 month post-injury, resulted in greater muscle mass, muscle contractility, and permeabilized muscle fiber mitochondrial respiration compared to untreated VML-injured mice. Histologically, these mice had evidence of greater total muscle fiber number and oxidative fibers; however, they also had a greater percentage of densely packed collagen. The regenerative medicine model produced greater permeabilized muscle fiber mitochondrial respiration compared to untreated VML-injured mice; however, the non-biodegradable biomaterial was associated with fewer total muscle fibers and lower muscle quality (i.e. lower muscle mass-normalized contractility). The conclusions reached from this study are: (i) regenerative rehabilitation and regenerative medicine strategies utilizing formoterol require further optimization but showed promising outcomes; and (ii) in general, β-adrenergic receptor agonism continues to be a physiologically supportive intervention to improve muscle contractile and metabolic function after VML injury.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf015"},"PeriodicalIF":5.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12007732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in copper-containing biomaterials for managing bone-related diseases.","authors":"Kunwei Li, Huan Cao, Hao Huang, Songyuan Tang, Han Wang, Qing Yang, Yonghe Hu, Jie Weng, Xin Chen","doi":"10.1093/rb/rbaf014","DOIUrl":"https://doi.org/10.1093/rb/rbaf014","url":null,"abstract":"<p><p>Bone-related diseases pose a major challenge in contemporary society, with significant implications for both health and economy. Copper, a vital trace metal in the human body, facilitates a wide range of physiological processes by being crucial for the function of proteins and enzymes. Numerous studies have validated copper's role in bone regeneration and protection, particularly in the development and expansion of bone collagen. Owing to copper's numerous biological advantages, an increasing number of scientists are endeavoring to fabricate novel, multifunctional copper-containing biomaterials as an effective treatment strategy for bone disorders. This review integrates the current understanding regarding the biological functions of copper from the molecular and cellular levels, highlighting its potential for bone regeneration and protection. It also reviews the novel fabrication techniques for developing copper-containing biomaterials, including copper-modified metals, calcium phosphate bioceramics, bioactive glasses, bone cements, hydrogels and biocomposites. The fabrication strategies and various applications of these biomaterials in addressing conditions such as fractures, bone tumors, osteomyelitis, osteoporosis, osteoarthritis and osteonecrosis are carefully elaborated. Moreover, the long-term safety and toxicity assessments of these biomaterials are also presented. Finally, the review addresses current challenges and future prospects, in particular the regulatory challenges and safety issues faced in clinical implementation, with the aim of guiding the strategic design of multifunctional copper-based biomaterials to effectively manage bone-related diseases.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf014"},"PeriodicalIF":5.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and functional evaluation of recombinant type III collagen intrauterine implant gel.","authors":"Xinhui Wang, Xiaoju Fan, Yuanxin Zhai, Jie Li, Huilin Sun, Jie Li, Hao Le, Feng Zhang, Li Zhang, Jianhao Wang, Yun Chu, Pengfei Cui","doi":"10.1093/rb/rbaf013","DOIUrl":"10.1093/rb/rbaf013","url":null,"abstract":"<p><p>Intrauterine adhesion (IUA) is a prevalent complication arising from uterine surgery, significantly impacting women's fertility and overall quality of life. The conventional clinical approach involves hysteroscopic separation of uterine adhesions, though this method poses operational challenges and carries risks of postoperative re-adhesion. Alternatively, the intraoperative placement of intrauterine devices or support balloons can act as a physical barrier to prevent adhesion formation. However, its effectiveness is limited and it may result in secondary damage to the endothelial tissue. To tackle these challenges, we have engineered a temperature-responsive hydrogel incorporating Pluronic HP407/HP188 pharmaceutical excipients and recombinant type III collagen (rCol III) as a bioactive element. Upon <i>in situ</i> injection into the uterine cavity, this hydrogel transitions from a sol-gel phase to a gel in response to body temperature changes, thereby minimizing nonspecific distribution and prolonging the duration of treatment. <i>In vitro</i> studies demonstrate that rCol III temperature-responsive hydrogels exhibit favorable biocompatibility, exhibit a recruitment effect on human endometrial stromal cells, suppress the expression of the fibrotic factor transforming growth factor beta 1 and promote angiogenesis. To evaluate its efficacy in preventing IUA via <i>in vivo</i> experiments, we employed sexually mature female rats for IUA modeling and compared its performance with a commercially available product, cross-linked sodium hyaluronate gel. The results indicate that rCol III temperature-responsive hydrogels significantly enhance retention at the injury site, substantially promote endometrial regeneration, augment endometrial blood supply and reduce abnormal fibrin deposition. This study suggests that rCol III temperature-responsive hydrogels can effectively prevent post-surgical uterine adhesions, highlighting their potential as a promising adhesion prevention strategy.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf013"},"PeriodicalIF":5.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation behavior of porous magnesium alloy scaffold under the low-intensity pulsed ultrasound intervention and their effect on bone defects repair.","authors":"Delin Ma, Mingran Zheng, Jun Wang, Yuan Zhang, Qichao Zhao, Zhaotong Sun, Junfei Huang, Wenxiang Li, Shijie Zhu, Liguo Wang, Xiaochao Wu, Shaokang Guan","doi":"10.1093/rb/rbaf011","DOIUrl":"10.1093/rb/rbaf011","url":null,"abstract":"<p><p>Biodegradable porous magnesium alloy (pMg) scaffolds hold significant potential for repair of bone defects owing to favorable mechanical properties and biocompatibility. However, a critical challenge remains in matching the degradation rate of pMg scaffolds with the pace of bone regeneration. Low-intensity pulsed ultrasound (LIPUS) has emerged as a promising therapeutic strategy to enhance bone repair. In this study, femoral bone defects in Sprague-Dawley rats were implanted with pMg scaffolds, and LIPUS was applied to the defect sites post-operatively. This study primarily investigated the degradation behavior of pMg scaffolds <i>in vivo</i> experiments, as well as their reparative effects on bone defects under LIPUS intervention. <i>In vivo</i> analysis revealed that LIPUS intervention accelerated the degradation of pMg scaffolds by loosening the degradation layer, making it more susceptible to erosion. Concurrently, LIPUS enhanced the accumulation of beneficial calcium and phosphorus compounds on the surface of the pMg scaffolds. Furthermore, the pMg + LIPUS group exhibited enhanced bone formation and mineralization around the degradation site compared to the pMg group alone, attributed to the increasing osteocalcin (OCN) and type I collagen (COL-I) as well as reduction in osteolysis by pMg and LIPUS-induced osteogenesis effect. At the 24-week post-surgery, the hardness value (HV) of regeneration bone in the pMg + LIPUS group had a 15% increase compared to the pMg group and approached the HV of healthy bone. In conclusion, the promotion of bone tissue growth rate under the intervention of LIPUS in conjunction with the degradation rate of pMg scaffolds offers a novel clinical strategy for the repair of bone defects.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf011"},"PeriodicalIF":5.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}