Regenerative Biomaterials最新文献

{"title":"Strontium/magnesium-doped coralline hydroxyapatite for bone regeneration.","authors":"Bixiu Chen, Liyan Zhang, Zhou Zhong, Chunyu Liu, Haobo Pan","doi":"10.1093/rb/rbaf036","DOIUrl":"10.1093/rb/rbaf036","url":null,"abstract":"<p><p>The biocompatibility, osteoconductivity and porous structure of coral make it a popular material for bone regeneration. However, coral mismatches host bone degradation rates and lacks osteoinductivity. No prior research has investigated the physicochemical properties of strontium-doped coralline hydroxyapatite (Sr-CHA), magnesium-doped (Mg-CHA) and strontium- and magnesium-co-doped (Sr-Mg-CHA), especially their osteogenic mechanisms. This study synthesized CHA doped with osteoinductive elements (Sr, Mg and Sr-Mg) via a hydrothermal reaction to preserve 26.5-33.5% of the unconverted inner core of calcium carbonate (CaCO₃). Under identical reaction circumstances, the Sr doping ratio in the Sr-CHA outperformed Mg in the Mg-CHA. In contrast, Sr and Mg mutually inhibit each other during co-doping in the Sr-Mg-CHA. The Sr-CHA nanorods on nanocluster spheres were the longest, while the Mg-CHA were the shortest, with the Sr-Mg-CHA occupying an intermediate length. The Sr-CHA, Mg-CHA and Sr-Mg-CHA exhibited 16 times the specific surface area and 14 times the pore volume of the coral and displayed better biocompatibility and expression levels of osteogenesis-related genes and proteins (e.g. ALP, Runx2, COL I, OCN and OPN) compared to coral <i>in vitro</i>, as well as improved osteogenesis than coral or Bio-Oss^®  <i>in vivo</i>. With its optional Sr²⁺ release concentration and degradation rates and large specific surface area and pore volume, the Sr-CHA performs the best. This study improved bone tissue engineering and regenerative medicine by enhancing the understanding of doped CHA and revealing new ways to overcome bone repair material problems.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf036"},"PeriodicalIF":8.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12362243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis boosting system based on a sonodynamic therapy cascade-augmented strategy for triple-negative breast cancer therapy.","authors":"Juying Zhang, Hanmei Li, Litao Ye, Yihan Leng, Xiaoqing Wang, You Yang, Qiong Jiang, Linli Feng, Ling Li, Yang Li, Jinhong Yu","doi":"10.1093/rb/rbaf042","DOIUrl":"10.1093/rb/rbaf042","url":null,"abstract":"<p><p>One of the novel forms of programmed cell death, ferroptosis, has recently emerged as a hopeful treatment strategy for triple-negative breast cancer (TNBC). However, insufficient levels of intracellular reactive oxygen species (ROS) and high levels of ROS scavengers in the tumor microenvironment (TME), such as glutathione (GSH), hamper the efficacy of ferroptosis therapy. In this study, the introduction of manganese dioxide nanoparticles (MnO₂ NPs) generated cytotoxic hydroxyl radicals (⋅OH) in the TME. Importantly, MnO₂ NPs act as a nanosensitizer by consuming H₂O₂/GSH in the TME, generating oxygen (O₂) to relieve the oxygen deficiency of tumors, induce tumor oxidative stress and ultimately enhance SDT-induced ferroptosis. Additionally, oxygen, as an ultrasound contrast agent, enables the visualization of the TNBC treatment process. Meanwhile, GSH depletion in the TME leads to failure of the major cellular system defending against ferroptosis, which also promotes the accumulation of lipid peroxidation in tumor tissue. Specifically, robust autophagy induced by ROS enhances the intracellular iron pool by breaking down ferritin, thereby promoting ferroptosis in cancer cells, leading to the optimal antitumor effect. Consequently, a ferroptosis boosting system that simultaneously encapsulates MnO₂ NPs and chlorin e6 (Ce6) was constructed for the intervention of TNBC. Both the <i>in vitro</i> and <i>in vivo</i> results demonstrated that Ce6-MnO₂-BSA nanoparticles can generate a significant ROS storm under ultrasound irradiation, eliminating GSH and inducing an autophagic response that increases the effectiveness of ferroptosis, thus, inhibiting the growth of TNBC without obvious toxic side effects. This effective strategy can cascade-augment cancer cell ferroptosis, providing a new perspective for the clinical treatment of TNBC.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf042"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of EGCG/chlorhexidine functionalized coating to reinforce the soft tissue seal at transmucosal region of implants.","authors":"Lijie Zhang, Tiancheng Gao, Huaxue Qu, Bolin Li, Yuan Li, Yi Zhang, Tianxiang Dai, Tianshuo Zhu, Wei Li, Weibo Zhang, Jialong Chen, Xiangyang Li","doi":"10.1093/rb/rbaf046","DOIUrl":"10.1093/rb/rbaf046","url":null,"abstract":"<p><p>Recent advancements in dental implant technology have provided more reliable and durable solutions for patients. Soft tissue seal (STS) is crucial for achieving implant stability, maintaining tissue health and promoting integration with surrounding soft and hard tissues. However, the STS around implants is fragile and susceptible to disruption by oral pathogens, particularly in patients with periodontitis or poor oral hygiene, leading to complications such as peri-implant mucositis and peri-implantitis. To promote STS formation, it is crucial to maintain the balance between bacterial and host cells while effectively managing inflammation. Although titanium-based implants exhibit biocompatibility, they lack inherent antibacterial and anti-inflammatory properties. To address these challenges, we developed a dual-function antibacterial and anti-inflammatory coating using chlorhexidine (CHX) and epigallocatechin gallate (EGCG). CHX effectively reduces bacterial adhesion but may inhibit fibroblast proliferation, while EGCG provides antioxidant and anti-inflammatory benefits. Three types of EGCG/CHX composite coatings were developed on titanium surfaces at different pH values. These coatings exhibited enhanced bacterial resistance, reduced inflammation and ROS scavenging capabilities, with higher pH levels further improving their performance. <i>In vivo</i> studies also confirmed that these coatings effectively prevented bacterial adhesion, mitigated inflammation and promoted STS formation, thereby holding significant promise for enhancing the long-term success of dental implants.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf046"},"PeriodicalIF":8.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12304417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144744485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a mechanical adaptable, moisture retention capable, injectable and adhesive organohydrogel for nucleus pulposus repairing.","authors":"Yaping Wang, Dong Wang, Chu Gao, Chuxin Zhou, Xiao Lin, Di Wang, Liu Yang, Huan Zhou, Lei Yang","doi":"10.1093/rb/rbaf047","DOIUrl":"10.1093/rb/rbaf047","url":null,"abstract":"<p><p>Developing mechanical adaptable injectable gel with nucleus pulposus (NP) repairing capability for minimally invasive treatment of intervertebral disc degeneration (IDD) is of great importance in medical practice. In current work, inspired by the outcomes of polyvinyl alcohol and glycerol based injectable organohydrogel (GPG) in IDD control and the great potential of animal glue in tissue adhesion, a novel injectable and self-crosslinking adhesive organohydrogel GPG-AG was fabricated. The mechanical performance of the GPG-AG was systematically studied, possessing viscoelastic properties close to NP accompanied with strong adhesion to intervertebral disc to avoid dynamic loading induced leakage postinjection. In addition, the swelling behavior, water retention capability and degradation of the organohydrogel <i>in situ</i> was also explored. <i>In vitro</i> cellular test showed the as-fabricated organohydrogel was able to upgrade aggrecan expression while downregulate matrix metallopeptidase-13 (MMP-13) synthesis. Astoundingly, the organohydrogel revealed anti-inflammation potential of alleviating excessive reactive oxygen species, consequently creating a favored microenvironment for NP repairing. The corresponding <i>in vivo</i> study showed the outcome in intervertebral disc height index of the GPG-AG treated group after needle puncture was superior to previously reported GPG and control group. Taken together, this organohydrogel is expected to serve as a promising candidate for IDD control.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf047"},"PeriodicalIF":5.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitooligosaccharides accelarate myelin clearance by Wipi1 mediated Schwann cell autophagy promoting peripheral nerve regeneration.","authors":"Hongkui Wang, Miao Zhang, Mengke Liu, Jina Liu, Jiahuan Gong, Long Yin, Yumin Yang, Yahong Zhao","doi":"10.1093/rb/rbaf044","DOIUrl":"10.1093/rb/rbaf044","url":null,"abstract":"<p><p>As the most feasible method to reconstruct long-distance peripheral nerve injuries, tissue-engineered nerves rely on biomaterials as a key driving factor. Chitooligosaccharides, intermediate products of chitosan degradation, have the ability to positively regulate nerve regeneration microenvironments. However, the impact of chitooligosaccharides on clearance of myelin debris during Wallerian degeneration is unrevealed. The focus is on exploring the role of chitooligosaccharides in myelin clearance, which is a crucial preparation stage for nerve regeneration. The effects of chitooligosaccharides on nerve regeneration were demonstrated through the morphological and functional evaluations. Then, the myelin lipids and proteins were analyzed using the morphological staining, and molecular and protein detection. The microstructure and ultrastructure observations of lysosomes and autophagosomes were performed. In addition, the proteomics and bioinformatics analysis of injured nerves treated with chitooligosaccharides. The interacting molecules and the regulatory network of Wipi1 were further predicted. On the basis of positive roles on peripheral nerve regeneration, it was illustrated that chitooligosaccharides accelerated the clearance of myelin. Furthermore chitooligosaccharides could regulate lysosomal and autophagic functions, and its role in promoting myelin clearance was mainly related to the enhanced autophagy of Schwann cells rather than macrophages. The big data analysis revealed that Wipi1 was notably upregulated in Schwann cells, mediating chitooligosaccharides to promote autophagy and myelin clearance. Meanwhile, as a potential therapeutic target, Wipi1 significantly accelerated myelin clearance and lipid metabolism after peripheral nerve injury. Our research deepens the comprehensive understanding of the positive regulatory role of chitosan and chitooligosaccharides; and it expands new content and ideas for designing and constructing better tissue-engineered nerves from the perspective of mutual communication and response between biomaterials and body tissues.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf044"},"PeriodicalIF":8.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tartaric acid-branched polyethyleneimine carbon dots promote repair of bone defect via osteogenic differentiation.","authors":"Soon Chul Heo, Hae Won Shin, Dong Joon Lee, Franklin Garcia-Godoy, Bo Ram Keum, Yong Hoon Kwon, Hyung Joon Kim","doi":"10.1093/rb/rbaf030","DOIUrl":"10.1093/rb/rbaf030","url":null,"abstract":"<p><p>Treating bone defects is a critical challenge in regenerative medicine. Carbon nanomaterials, with their unique physicochemical properties, offer significant potential for enhancing bone regeneration. In this study, we developed tartaric acid (TA)-based carbon dots (CDs) by synthesizing TA with branched polyethyleneimine (bPEI). These TA-bPEI CDs were systematically evaluated to determine their effects on osteogenic differentiation in human bone marrow-derived mesenchymal stem cells (BMSCs) and their capacity to repair calvarial defects in an <i>in vivo</i> model. Characterization of TA-bPEI CDs revealed a size of approximately 10 nm and a positive surface charge. The CDs exhibited fluorescence emission peaks between 464 and 506 nm under excitation wavelengths of 340-440 nm. Cytotoxicity assays demonstrated that TA-bPEI CDs maintained BMSC viability at concentrations up to 250 μg/ml. However, at concentrations of 500 μg/ml and above, apoptosis was induced. Treatment with TA-bPEI significantly enhanced osteogenic differentiation <i>in vitro</i>, as evidenced by increased expression of osteogenic-specific proteins such as Runx2, ALP, OCN and OPN. <i>In vivo</i>, the application of TA-bPEI CDs in a mouse calvarial defect model promoted robust new bone formation, reduced defect gaps, and improved bone morphometric parameters, including bone volume fraction and trabecular thickness. These results suggest that TA-bPEI CDs enhance osteogenesis by directly stimulating osteogenic differentiation and upregulating osteogenesis-specific genes. This study demonstrates the high potential of TA-bPEI CDs as a novel nanomaterial for bone regeneration applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf030"},"PeriodicalIF":5.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant human fibronectin segment (rhFN₁₀₂₄) hydrogel carried hPDLSCs to repair diabetic trauma by activated NF-κB signaling pathway.","authors":"Jianhang Cong, Yating Cheng, Tongtong Liu, Xiang Cai, Jiahui Xu, Rui Guo, Rongrong He, Qi Xiang","doi":"10.1093/rb/rbaf027","DOIUrl":"10.1093/rb/rbaf027","url":null,"abstract":"<p><p>The accumulation of advanced glycation end products (AGEs) plays a crucial role in chronic inflammation and delayed wound healing in individuals with diabetes. In this context, fibronectin has been identified as a crucial protein that promotes the differentiation of human periodontal ligament stem cells (hPDLSCs) into myofibroblasts, which play a vital role in the repair of diabetic skin ulcers. This process is intimately associated with the integrin β1 receptor and the NF-κB signaling pathway, both crucial for cellular responses to fibronectin. To validate our hypothesis, we expressed rhFN₁₀₂₄, a recombinant protein containing the integrin β1 affinity-binding domain from human fibronectin segments 12-14. This protein was used to formulate a hydrogel for hPDLSCs. rhFN₁₀₂₄'s binding affinity to integrin β1 was confirmed by molecular docking and the cellular thermal shift assay (CETSA). We developed <i>sh-ITGB1</i>-hPDLSCs with stable ITGB1 knockdown using <i>shRNA-ITGB1</i> and compared their proliferation, migration and adhesion to wild-type hPDLSCs. Morphological changes were observed via SEM, and α-SMA expression levels were measured in AGEs-damaged hPDLSCs. We created full-thickness wound models in diabetic mice to assess pharmacodynamics. The study showed that rhFN₁₀₂₄ stimulated hPDLSCs differentiation into myofibroblasts by boosting ITGB1 expression. rhFN₁₀₂₄ also reduced AGEs' negative effects on hPDLSCs, as seen through SEM analysis and α-SMA levels. In full-thickness wound models, hPDLSCs and rhFN₁₀₂₄ accelerated re-epithelialization and collagen synthesis. rhFN₁₀₂₄ is proposed to interact with the ITGB1 receptor on hPDLSCs, activating the NF-κB pathway to neutralize AGEs-induced pro-inflammatory cytokines. This study suggests rhFN₁₀₂₄ as a potential biomedical material for tissue repair.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf027"},"PeriodicalIF":5.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Y-shaped DNA as a dynamic self-assembly nanomaterial for phenotype-specific regulation of stem cell differentiation on the gene level.","authors":"Wengang Liu, Ruili Liu, Lok Ting Chu, Xinlei Wang, Jianpeng Wu, Jiandong Ding, Ting Hsuan Chen","doi":"10.1093/rb/rbaf043","DOIUrl":"10.1093/rb/rbaf043","url":null,"abstract":"<p><p>While genetic engineering has offered new strategies for regulating stem cell differentiation, the efficacy varies in cells with different phenotypes or lineage commitments, leading to inconsistent differentiation outcomes and uncertainty in regenerative medicine. To address this issue, we employ a Y-shaped DNA (Y-DNA) as a nanomaterial to phenotype-specifically regulate differentiation of human mesenchymal stem cells (hMSCs). Y-DNA is composed of three DNA strands with complementary sequences and different roles. The Y-DNA designed in the present study can be uniquely activated by miR-106a-5p, a microRNA preferentially expressed in adipogenesis-biased hMSCs. Upon activation, the Y-DNA disassembles, releasing an antisense oligonucleotide that inhibits expression of cofilin, which serves as a key regulator to enhance adipogenic differentiation, and thus, prevents hMSCs from undergoing osteogenic differentiation. The key regulatory role of cofilin in hMSC differentiation is verified at the single-cell level on arginine-glycine-aspartate microislands under the nonfouling background of poly(ethylene glycol) hydrogels. Our strategy effectively redirects these cells towards osteogenic differentiation by inhibiting adipogenic differentiation, demonstrating dose dependence with high specificity, selectivity, and low toxicity. hMSCs cultured in a dual induction medium (a mixture of adipogenic medium and osteogenic medium) show enhanced osteogenic differentiation after transfection with the nanostructured Y-DNA. This approach addresses the challenge of cell heterogeneity in bone regeneration, offering a promising solution for precise control over stem cell fate. The ability of Y-DNA to specifically target cells with a propensity for adipogenic differentiation and to reprogram their lineage commitment has significant implications for the field of regenerative medicine, particularly in applications requiring enhanced purity of cell differentiation outcomes.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf043"},"PeriodicalIF":5.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced prevention on postoperative atrial fibrillation by using anti-inflammatory biodegradable drug patch.","authors":"Pengcheng Yu, Weiqi Lu, Huaxin Sun, Chengchen Huang, Xiaolin Zhou, Yuxing Wang, Zhen Zhang, Guosheng Fu, Hanxiong Liu, Kefeng Ren, Xia Sheng","doi":"10.1093/rb/rbaf040","DOIUrl":"10.1093/rb/rbaf040","url":null,"abstract":"<p><p>Postoperative atrial fibrillation (POAF) is the most prevalent form of secondary atrial fibrillation and increases the risk of adverse cardiovascular outcomes, such as stroke, heart failure and increased mortality. Herein, we designed an andrographolide (Andr)-loaded degradable polymer patch to deliver the drug directly to the atrial tissue for prevention of POAF. The sterile pericarditis (SP) rat model was adopted for highly relationship to clinical practice. The patch-released Andr effectively reduced the incidence of atrial fibrillation from 90 to 20%, and alleviated local atrial inflammation and oxidative stress <i>in vivo</i>, by using electrophysiological detection and histological analysis such as immunofluorescence, western blot and PCR. In HL-1 cells, we found the use of Andr-loaded patch could strongly inhibit the cell death, reactive oxygen species (ROS) generation and mitochondrial injury caused by LPS. Meanwhile, the use of Andr-loaded patch could effectively inhibited macrophages polarize towards M1. Mechanistically, we verified that the regulation of the cytoplasm and mitochondria Ca²⁺ and ROS dynamic balance was quite important both <i>in vivo</i> and <i>in vitro</i>. Our strategy proved by regulating the inflammatory microenvironment, ROS balance and Ca²⁺ homeostasis and the Andr-loaded atrial patch was effective for POAF in the SP rat model. The electrical signal of atrial stromal reentry in the case of this model was successfully mined, and the results of calcium channel were basically consistent with that of electrical signal channel. In addition, we have reported the infiltration and polarization of local inflammatory cells in the atrial of POAF at the tissue section level. Our study served as a new inspiration for the treatment of arrhythmic diseases and other ROS- and Ca²⁺- associated local illnesses.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf040"},"PeriodicalIF":5.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design matrix materials for organoid development and application in biomedicine.","authors":"Yue Huang, Xiaoyu Zhang, Wanjun Zhang, Jinglong Tang, Jing Liu","doi":"10.1093/rb/rbaf038","DOIUrl":"10.1093/rb/rbaf038","url":null,"abstract":"<p><p>Organoids are three-dimensional tissue analogues grown <i>in vitro</i>. Although they are not human organs in the strict sense, they can mimic the structure and function of tissues <i>in vivo</i> to the maximum extent, and have broad application prospects in the fields of organ development, personalized medicine, regenerative medicine, disease modeling, drug screening, gene editing, etc. There is even hope that organoids can replace experimental animals for preclinical testing, which will greatly shorten the cycle of preclinical testing and improve its efficiency. Nowadays, Matrigel remains the predominant substitute for organoid culture systems. At the same time, new extracellular matrix or inspired polymer materials with tunable and optimized biochemical and biophysical properties continue to emerge, which are of great significance for efficient and high-level cultivation of organoids. In this review, we critically evaluate how mechanobiological signaling dynamics at the cell-matrix interface inform the rational engineering of biomimetic extracellular matrices to achieve standardized and phenotypically regulated patient-derived organoid cultures. Then, we systematically classify hydrogel-based matrices encompassing natural, biohybrid, synthetic, protein-engineered and DNA crosslinked matrix systems by their biocompatibility and functional compatibility. Focusing on cancer oncogenesis and progression research, drug development and personalized medicine, we highlight biomimetic hydrogel innovations that recapitulate tumor organoids development. By summarizing the obstacles that hinder the development of organoid hydrogels, we hope to provide an outlook on the future directions for the development of organoid hydrogels and promote the application of organoids in the field of biomedicine.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf038"},"PeriodicalIF":5.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}