Regenerative Biomaterials最新文献

{"title":"A novel iron bioresorbable scaffold: a potential strategy for pulmonary artery stenosis.","authors":"Li Qin, Gui Zhang, Ling Sun, Zhijin Yu, Zhe Zhang, Lifeng Sun, Wanqian Zhang, Wenchao Fu, Yetao Ou, Wenjing Zhang, Xiaoli Shi, Zhixiang Si, Jingfang Shen, Limei Cha, Zhiwei Zhang, Deyuan Zhang","doi":"10.1093/rb/rbaf041","DOIUrl":"10.1093/rb/rbaf041","url":null,"abstract":"<p><p>A big diameter bioresorbable scaffold is expected to be used for treatment of vessel stenosis of children with congenital heart disease to adapt the growth characteristics of vessel of children and avoid the late adverse events of permanent stent implanted in children. However, it is challenging to fabricate a big diameter bioresorbable scaffold that is appropriate for percutaneous implantation with enough mechanical performance and can be smoothly delivered in children's small vessel. In this study, a novel iron big and bioresorbable Scaffold (BBS) for pulmonary artery stenosis of children with congenital cardiovascular diseases was fabricated and evaluated. The BBS was made of nitrided iron tube and processed by laser cutting and polishing. The testing results of radial strength, recoil, shortening, maximal expansion diameter and side-branch accessability illustrated the BBS has good mechanical performance. The animal study showed that the percentage of area stenosis of BBSs was 18.1 ± 8.6%, 20.2 ± 5.9% and 20.4 ± 6.1% at 28, 90 and 180 days after implantation in 17 rabbits, and no malposition, thrombus, dissection or tissue necrosis in the rabbit model was detected by micro-CT, STEM and histological examinations. An φ8 × 23 mm BBS was implanted into a 55-month-old child with left pulmonary stenosis, and multiple spiral CT was conducted. No lumen area loss appeared at 1- and 2-year follow-ups in this first-in-man study. It suggested that the BBS might be a new strategy for the therapy of pulmonary artery stenosis in children.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf041"},"PeriodicalIF":5.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable hydrogel loaded with exosomes from hypoxic umbilical cord-derived mesenchymal stem cells alleviates intervertebral disc degeneration by reversing nucleus pulposus cell senescence.","authors":"Xin Zhao, Yubo Shi, Zhen Sun, Wei Duan, Le Chang, Benchi Xu, Kangwei Lai, Jingchun Zhang, Buqi Tian, Weidong Tao, Zhenzhou Mi, Mian Zhang, Wenjing Yang, Zhuojing Luo, Zhengxu Ye","doi":"10.1093/rb/rbaf039","DOIUrl":"10.1093/rb/rbaf039","url":null,"abstract":"<p><p>Intervertebral disc degeneration is a significant contributor to the development of spinal disorders. Previous studies have shown that the senescence of nucleus pulposus cells can worsen the degradation of intervertebral disks. Therefore, targeting the senescence of nucleus pulposus cells may be a promising therapeutic approach for the treatment of intervertebral disc degeneration. This study investigated the use of exosomes from hypoxic umbilical cord-derived mesenchymal stem cells to reverse nucleus pulposus cells senescence and delay intervertebral disc degeneration progression. MicroRNA sequencing of hypoxic umbilical cord-derived mesenchymal stem cells revealed the presence of functional microRNAs, with the p53 signalling pathway identified as a key factor. To enhance the release time of hypoxic umbilical cord-derived mesenchymal stem cells <i>in vivo</i>, hyaluronic acid methacryloyl hydrogel was used to load hypoxic umbilical cord-derived mesenchymal stem cells and create a sustained-release system. This system effectively repaired the degradation of the extracellular matrix, reversed nucleus pulposus cells senescence and alleviated intervertebral disc degeneration progression in a rat model. Overall, this study highlights the potential of hypoxic umbilical cord-derived mesenchymal stem cells in reducing nucleus pulposus cell senescence and suggests the possibility of combining it with a sustained-release system as a novel therapeutic strategy for intervertebral disc degeneration.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf039"},"PeriodicalIF":5.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the maturity of <i>in vitro</i> engineered cartilage from Wharton's jelly-derived photo-crosslinked hydrogel using dynamic bioreactors and its <i>in vivo</i> outcomes in animal models.","authors":"Chuanzhi Wei, Mingyue Lin, Qitao Bo, Wufei Dai, Jinghao Ding, Ru Chen","doi":"10.1093/rb/rbaf037","DOIUrl":"10.1093/rb/rbaf037","url":null,"abstract":"<p><p>The immature state of <i>in vitro</i> engineered cartilage (IVEC) hinders its clinical translation, highlighting the need for optimized scaffold platforms and cultivation models. Our previous work demonstrated that Wharton's jelly (WJ) contains an extracellular matrix (ECM) whose composition closely resembles that of native cartilage and includes several bioactive factors that promote chondrogenic induction. Furthermore, earlier studies have shown that photo-crosslinkable hydrogels are ideal carrier scaffolds for cartilage tissue engineering and that bioreactors improve nutrient and waste exchange between scaffolds and the culture medium. Based on these findings, we employed a dynamic bioreactor in combination with a WJ-derived photo-crosslinkable hydrogel to enhance IVEC maturity. Our results indicate that the decellularized WJ matrix (DWJM) effectively retains its native chondrogenic ECM components and bioactive factors. The photo-crosslinkable ADWJM hydrogel-produced by modifying DWJM with methacrylate anhydride-demonstrated excellent gelation capacity as well as tunable rheological properties, swelling ratios and degradation rates across different DWJM concentrations. In addition, the ADWJM hydrogel exhibited outstanding biocompatibility by providing a favorable 3D microenvironment for chondrocyte survival and proliferation. Most importantly, the dynamic bioreactor markedly promoted IVEC maturation. Constructs cultured under dynamic conditions displayed increased thickness, wet weight and volume; enhanced mechanical strength; more typical lacunae structures; and uniform deposition of cartilage-specific ECM compared to constructs maintained in static conditions or within a static bioreactor. Moreover, in vivo subcutaneous implantation of IVEC in goats further validated these findings, as the implanted constructs exhibited cartilage components and mechanical properties closely resembling those of natural cartilage. These results offer a promising approach for enhancing IVEC maturity and support its future clinical translation.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf037"},"PeriodicalIF":5.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An acid-responsive bone-targeting nanoplatform loaded with curcumin balances osteogenic and osteoclastic functions.","authors":"Minhao Liang, Lei Zhou, Juan Li, Bin Liang, Liangyun Zhou, Fengfeng Xue, Libo Jiang, Wei Hong","doi":"10.1093/rb/rbaf028","DOIUrl":"10.1093/rb/rbaf028","url":null,"abstract":"<p><p>Postmenopausal osteoporosis (PMOP) is a predominant form of clinical osteoporosis. It has led to significant health and social burdens for older patients. Reestablishing the balance between osteogenic and osteoclastic is a crucial strategy for treating PMOP. Curcumin (Cur), a naturally derived polyphenolic substance, has gained recognition as a viable option for treating osteoporosis. Despite its potential, the clinical use of Cur is hindered by its limited bioavailability and the presence of side effects. Nanoparticles modified with aspartic acid octapeptide (ASP8) exhibit a strong affinity for bone tissue, facilitating targeted delivery. This study presents novel acid-responsive zeolite imidazolate framework-8 (ZIF) nanoparticles modified with ASP8 and loaded with Cur (Cur@ZIF@ASP8, CZA). Upon delivery by this nanoparticle drug delivery system, Cur can effectively regulate bone homeostasis, offering a potential therapeutic strategy for osteoporosis. This study demonstrated that CZA nanoparticles could successfully transport Cur to bone tissue without significant toxicity. Furthermore, nanoparticles promote bone formation and inhibit osteoclast activity. They also modify the expression of related genes and proteins, such as OCN, ALP, CTSK and MMP9. Significant evaluations utilizing microcomputed tomography, Masson's staining, hematoxylin and eosin staining and immunofluorescence staining demonstrated that intravenous CZA administration in ovariectomized mice resulted in bone destruction while simultaneously reducing overall bone loss. In conclusion, CZA nanoparticles hold promise as a therapeutic option for osteoporosis.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf028"},"PeriodicalIF":5.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed Gel/PTH@PAHA scaffolds with both enhanced osteogenesis and mechanical properties for repair of large bone defects.","authors":"Zhimou Zeng, Ping Song, Xingyu Gui, Bicheng Ake, Taoyu Liu, Hao Liu, Linnan Wang, Lei Wang, Yueming Song, Bo Qu, Changchun Zhou","doi":"10.1093/rb/rbaf029","DOIUrl":"10.1093/rb/rbaf029","url":null,"abstract":"<p><p>The repair of large bone defects continues to pose a significant challenge in clinical orthopedics. Successful repairs require not only adequate mechanical strength but also exceptional osteogenic activity for successful clinical translation. Composite materials based on polyamide 66 (PA66) and hydroxyapatite have been widely used in various clinical settings. However, existing PA66/hydroxyapatite composites often lack sufficient osteogenic stimulation despite their favorable mechanical properties, which limit their overall clinical efficacy. In this study, we fabricated a polyamide 66/nano-hydroxyapatite (PAHA) scaffold using an extruder and fused deposition modeling-based 3D printing technology. Subsequently, gelatin methacrylamide (GelMA) containing teriparatide (PTH) was incorporated into the PAHA scaffold to construct the Gel/PTH@PAHA scaffold. Material characterization results indicated that the compressive modulus of elasticity and compressive strength of the Gel/PTH@PAHA scaffold were 172.47 ± 5.48 MPa and 25.55 ± 2.19 MPa, respectively. <i>In vitro</i> evaluations demonstrated that the Gel/PTH@PAHA scaffold significantly enhanced osteoblast adhesion and proliferation while promoting osteogenic differentiation of BMSCs. <i>In vivo</i> studies further revealed that this scaffold notably promoted new bone regeneration in rabbit femoral defects. These findings suggest that the 3D-printed Gel/PTH@PAHA scaffold exhibits excellent mechanical properties alongside remarkable osteogenic activity, thereby meeting the dual requirements for load-bearing applications and bone regeneration. This innovative approach may be a promising candidate for customized orthopedic implants with substantial potential for clinical application.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf029"},"PeriodicalIF":5.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing electrospinning towards the future of biomaterials in biomedical engineering.","authors":"Yanjiao Teng, Lin Song, Jie Shi, Qi Lv, Shike Hou, Seeram Ramakrishna","doi":"10.1093/rb/rbaf034","DOIUrl":"10.1093/rb/rbaf034","url":null,"abstract":"<p><p>Biomaterial is a material designed to take a form that can direct, through interactions with living systems, the course of any therapeutic or diagnostic procedure. Growing demand for improved and affordable healthcare treatments and unmet clinical needs seek further advancement of biomaterials. Over the past 25 years, the electrospinning method has been innovated to enhance biomaterials at nanometer and micrometer length scales for diverse healthcare applications. Recent developments include intelligent (smart) biomaterials and sustainable biomaterials. Intelligent materials can sense, adapt to and respond to external stimuli, autonomously adjusting to enhance functionality and performance. Sustainable biomaterials possess several key characteristics, including renewability, a low carbon footprint, circularity, durability, biocompatibility, biodegradability and others. Herein, advances in electrospun biomaterials, encompassing process innovations, working principles and the effects of process variables, are presented succinctly. The potential of electrospun intelligent biomaterials and sustainable biomaterials in specific biomedical applications, including tissue engineering, regenerative medicine, drug delivery systems, brain-computer interfaces, biosensors, personal protective equipment and wearable devices, is explored. More effective healthcare demands further advancements in electrospun biomaterials. In the future, the distinctive characteristics of intelligent biomaterials and sustainable biomaterials, integrated with various emerging technologies (such as AI and data transmission), will enable physicians to conduct remote diagnosis and treatment. This advancement significantly enhances telemedicine capabilities for more accurate disease prediction and management.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf034"},"PeriodicalIF":5.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sr-doped surfaces with 2D black phosphorus nanosheets for enhanced photothermal antibacterial activity and zirconia implant osseointegration.","authors":"Huan Cheng, Jiaquan Chen, Yan Wang, Yinyan Zhang, Tianyun Qin, Haobo Sun, Wen Si, Ningyao Sun, Yingyue Sun, Lifeng Xiong, Zhennan Deng, Lei Lu, Peng Gao, Jinsong Liu","doi":"10.1093/rb/rbaf033","DOIUrl":"10.1093/rb/rbaf033","url":null,"abstract":"<p><p>Zirconia (ZrO₂) has emerged as a preferred material for dental implants due to its excellent chemical inertness, absence of metal allergies and esthetic appeal. However, its limited bioactivity regarding infection resistance and early osseointegration hinders its implantation success rate compared to titanium implants. Herein, we developed a PDPA@Sr/BP coating for ZrO₂ implants to address these limitations. First, inspired by the adhesive properties of mussel foot proteins, a PDPA@Sr coating enriched with positively charged amine groups and strontium (Sr) ions was applied to the ZrO₂ surface. This coating stably anchored black phosphorus (BP) to the implant, effectively regulating its degradation rate and ensuring long-lasting antibacterial properties. Under near-infrared (NIR) light irradiation, BP generated localized heat, efficiently killing bacteria. Simultaneously, the release of Sr and phosphate ions from the PDPA@Sr/BP coating promoted bone formation and enhanced osseointegration. This study systematically evaluated the antibacterial effects and osseointegration-promoting properties of the PDPA@Sr/BP coating through both <i>in vitro</i> and <i>in vivo</i> experiments. The results demonstrated that compared to untreated ZrO₂ surfaces, the coating significantly enhances the implant's antibacterial properties and accelerates its surface osseointegration. This study proposes an innovative strategy to improve the clinical performance of ZrO₂ implants, demonstrating substantial potential for clinical translation.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf033"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels.","authors":"Jun Liu, Qian Liu, Minmei Guo, Chengyu Jiang, Jianyang Chen, Ting Wang, Tzu-Cheng Sung, Shih-Jie Chou, Shih-Hwa Chiou, Guoping Fan, Akon Higuchi","doi":"10.1093/rb/rbaf035","DOIUrl":"10.1093/rb/rbaf035","url":null,"abstract":"<p><p>A variety of novel peptide-grafted hydrogels, of which peptides were derived from vitronectin (PQVTRGDVFTMP) or the laminin β4 chain (PMQKMRGDVFSP), were prepared in this study. The peptide-grafted hydrogels promoted the adhesion, proliferation and colony formation of hiPSCs and maintained their pluripotency up to passage 5 under xeno-free conditions. We successfully generated RPE cells from hiPSCs using one of the most suitable xeno-free peptide-grafted hydrogels, KVN2CK (KGCGGKGG-PQVTRGDVFTMP), which was derived from vitronectin, and confirmed the effect of these hiPSC-derived RPE cells in a rat retinal degeneration model (Royal College of Surgeons (RCS) rats) via subretinal transplantation, when we investigated functional improvements in vision in RCS rats after the transplantation of hiPSC-derived RPE cells. Our novel peptide-grafted hydrogels provided a safe and robust platform for generating single-layer hiPSC-derived RPE cells under xeno-free conditions, which indicates the potential of these hydrogels for stem cell therapy for retinal degenerative diseases in the future.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf035"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnesium as an emerging bioactive material for orthopedic applications: bedside needs lead the way from innovation to clinical translation.","authors":"Ningze Zhang, Qida Zhang, Hongwei Shao, Zhengming Shan, Jiankun Xu, Wenxue Tong, Ronald Man Yeung Wong, Ling Qin","doi":"10.1093/rb/rbaf032","DOIUrl":"10.1093/rb/rbaf032","url":null,"abstract":"<p><p>With the rapid increase in population aging, the number of surgical operations in orthopedics is expected to increase. The gap between the materials applied in clinical orthopedics and materials in discovery and research is obvious due to regulatory requirements for biosafety and treatment efficacy. For the bedside needs, it is important to overcome hurdles by achieving impactful innovation and clinical translation of orthopedic materials. Magnesium (Mg), as an emerging bioactive material, is one of the vital components of the human body and mainly stored in the musculoskeletal system as either a matrix component or an intracellular element for the homeostasis of various physiological functions. However, the degradation and biomechanical performance limit the applications of Mg. This review aims to explore the current challenges and future directions of Mg for clinical translation and provide an update on biomaterials used in orthopedics, factors driving orthopedic innovation, physiology of magnesium ions (Mg²⁺) and its potential clinical applications. To achieve orthopedic application, modification of the performance of Mg as implantable metals and function of the degradation products of Mg <i>in vivo</i> are described. For the clinical needs of treating the steroid-associated osteonecrosis (SAON), Mg screws and Mg-based composite porous scaffolds (Mg/PLGA/TCP: magnesium/poly(lactic-co-glycolic acid) (PLGA)/tricalcium phosphate (TCP)) have been developed, but the challenges of Mg-based implants in load-bearing skeletal sites still exist. To utilize the beneficial biological effects of Mg degradation and overcome the weakness in mechanical stability for fracture fixation, the concept of developing Mg/titanium (Ti) hybrid orthopedic implants is reported, where the Ti component provides effective mechanical support while the inclusion of Mg component potentially optimizes the biomechanical properties of Ti component and facilitate bone healing. This review provides a reference frame for the translation of novel materials and promotes the development of innovative orthopedic biomaterials for clinical applications.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf032"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on osteoclast regulation by biodegradable magnesium and its mechanism.","authors":"Wangwei Zhu, Weidan Wang, Xing Yang, Chunxiao Ran, Tianwei Zhang, Shibo Huang, Jiahui Yang, Fuyang Wang, Huiya Wang, Peng Wan, Fengyuan Piao, Faqiang Lu, Shengbo Shi, Ye Li, Xiuzhi Zhang, Dewei Zhao","doi":"10.1093/rb/rbaf026","DOIUrl":"10.1093/rb/rbaf026","url":null,"abstract":"<p><p>Continuous advancements in medical technology and biomaterials have underscored the significant advantages of biodegradable implant materials for bone repair and remodelling over traditional inert metallic implants. Notably, biodegradable magnesium-based materials have gained much attention because of their optimal corrosion rates. Importantly, extensive clinical experience has resulted in the use of biodegradable magnesium-based orthopaedic implants. Both preclinical and clinical studies have consistently demonstrated that Mg has an excellent ability to promote bone tissue formation, a process that is closely associated with the release of Mg²⁺ and other degradation byproducts. Bone metabolism depends on a dynamic balance of bone formation and bone resorption. Mg²⁺ has been shown to increase osteoblast (OB) activity while suppressing osteoclast (OC) formation, thus playing a crucial role in bone remodelling and regeneration. In terms of osteolysis inhibition, Mg²⁺ plays a multifaceted role. First, Mg²⁺ inhibits OC formation by modulating the activity of mature OCs, their migratory behaviour and the activity of precursor cells. Second, Mg²⁺ influences OC production by regulating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor kappa-Β ligand (RANKL) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Additionally, Mg²⁺ impacts bone resorption by altering the immune microenvironment and the levels of hormones and peptides within the body. Furthermore, the alkaline environment generated around the biodegradable magnesium implant and its degradation products (e.g. H₂) also significantly inhibit OC formation. Recent research on magnesium-based implants has focused predominantly on their osteogenic properties, with few systematic reviews addressing the mechanisms through which biodegradable magnesium alloys suppress osteoclastic activity. This article summarizes the latest clinical research progress concerning biodegradable magnesium implant materials and their significant regulatory effects and discusses recent advances in the understanding of the regulatory mechanisms of action Mg-based biomaterials on OCs, with the aim of providing a more theoretical basis for the clinical application of biodegradable magnesium-based implants.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf026"},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}