Reproductive Sciences最新文献

{"title":"Rosiglitazone-Mediated Activation of PPARγ Induces PlGF Expression in Trophoblast Cells.","authors":"Pinki Nandi, Chidambra Halari, Mavis Lee, Elakkiya Prabaharan, Shahil Sarajideen, Dennis K Lee, Sascha Drewlo","doi":"10.1007/s43032-025-01868-w","DOIUrl":"10.1007/s43032-025-01868-w","url":null,"abstract":"<p><p>Preeclampsia (PE) is a hypertensive pregnancy disorder marked by impaired trophoblast invasion and placental vascular dysfunction, resulting in severe maternal and fetal complications. Placental growth factor (PlGF) is critical for proper placental angiogenesis and is transcriptionally regulated by glial cell missing-1 (GCM1), a downstream effector of peroxisome proliferator-activated receptor-gamma (PPARγ). Decreased PPARγ activity in PE may therefore contribute to diminished PlGF levels, worsening placental pathology. In this study, we investigated the mechanistic role of rosiglitazone, a PPARγ agonist, in rescuing PlGF expression under 1.5% oxygen/reoxygenation stress mimicking PE. Using JEG-3 trophoblast cells, we show that rosiglitazone enhances PPARγ nuclear translocation, leading to increased GCM1 and cyto-protective heme oxygenase-1 (HO-1) expression, and subsequent upregulation of PlGF production under both 21% oxygen and 1.5% oxygen/reoxygenation conditions. Pharmacologic inhibition of PPARγ with T0070907 or siRNA-mediated knockdown abrogated these effects, underscoring PPARγ's essential role in maintaining GCM1-driven PlGF expression. Notably, rosiglitazone treatment rescued PlGF production in 1.5% oxygen/reoxygenation-stressed cells, highlighting a potential therapeutic strategy to mitigate placental dysfunction. These findings define the PPARγ-GCM1-PlGF axis as a mechanistic cornerstone of placental health and suggest that pharmacological activation of PPARγ may offer clinical benefit in improving pregnancy outcomes in PE.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1864-1875"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is a Large Neck Circumference a Predictive Factor for Poor Semen Quality in the Turkish Population?","authors":"Duygu Dursunoglu","doi":"10.1007/s43032-025-01850-6","DOIUrl":"10.1007/s43032-025-01850-6","url":null,"abstract":"<p><p>Currently, the potential role of obesity on semen quality remains unclear. In recent years, alternative anthropometric indices to body mass index (BMI), which can precisely distinguish body fat distribution, have been described to estimate central adiposity. However, the role of neck circumference (NC), a novel index of central adiposity, on semen quality is unknown. This study aims to reveal the potentials of adiposity indices, including NC, in predicting poor semen quality. A total of 4739 male participants between the ages of 17-55 were included in this cross-sectional study. Semen parameters, including sperm concentration, total count, total, progressive and rapid progressive motility and vitality, were divided into two categories according to the WHO classification for male infertility. As anthropometric measurements of obesity, BMI and central obesity indices including waist circumference (WC), hip circumference (HC), waist-to-hip ratio (WHpR), waist-to-height ratio (WHtR) and NC were examined. The predictive potentials of obesity indices for low semen parameters were evaluated by Receiver Operating Characteristics (ROC) curve analysis. The associations of obesity indices with semen parameters were analyzed by binary logistic regression analyze after adjusting potential confounding factors. Patients with lower semen parameters had higher obesity indices than those with normal parameters. All obesity indices have the predictive potentials for low semen parameters. After adjustment for confounders, the strongest associations were found between HC with sperm count parameters, WC and WHtR with sperm vitality and motility parameters and WHpR and NC with rapid progressive motility. Obesity plays an important role in male infertility. Body fat distribution appears to have specific roles on sperm functionality, which may influence different infertility markers. NC is a strong predictor for sperm rapid progressive motility, suggesting a role for male infertility.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1840-1850"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher Pregnancy Success Rates in Patients with Diminished Ovarian Reserve < 40 Years When Initially Treated by Intrauterine Insemination with Mild Ovarian Stimulation Compared to In Vitro Fertilization Alone: A Pilot Study.","authors":"Julia J M Deneer, Saskia Le Cessie, Evert J P van Santbrink, Lucette A J van der Westerlaken, Eileen E L O Lashley","doi":"10.1007/s43032-025-01830-w","DOIUrl":"10.1007/s43032-025-01830-w","url":null,"abstract":"<p><p>For women with idiopathic diminished ovarian reserve (DOR), direct start with IVF has been suggested to potentially shorten the time to pregnancy. Others however prefer intra-uterine insemination with ovarian stimulation (IUI + OS) due to the expected low response in IVF. In this pilot study, we determined the effect of these two strategies in women with DOR < 40 years. From a retrospective cohort, we included 135 women that met the diagnostic criteria of DOR. Patients were randomly referred to two different outpatient clinics in the Netherlands between 2012-2018 because of subfertility. Primary outcome was clinical pregnancy; secondary outcomes included ongoing pregnancies, live births, time to pregnancy and pregnancy-related complications. An instrumental variable analysis was used to assess the average effect of treatment with IUI + OS followed by IVF (protocol A) compared to IVF alone (protocol B) and correct for (unknown) confounders. Treatment protocol A was performed in 72.6% patients in Centre 1 and 30.6% in Centre 2. In Centre 1 61.6% (45/73) women had a clinical pregnancy compared to 41.9% (26/62) in Centre 2 (difference 19.7% (95% CI 3.1%-36.3%), p = 0.02). Early miscarriage occurred in 24% of the women in Centre 1 in comparison to 45% of the women in Centre 2. There were no significant differences in pregnancy-related complications. This pilot study suggests that women < 40 with DOR, if treated with IUI + OS followed by IVF, have higher estimated cumulative clinical pregnancy success, with a trend towards higher ongoing pregnancies and live births, in comparison to women treated with IVF alone.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2010-2018"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postpartum Depression Increases the Risk of Childhood Attention Deficit Hyperactivity Disorder Diagnosis.","authors":"Darios Getahun, Michael J Fassett, Nana A Mensah, Nehaa Khadka, Meiyu Yeh, Vicki Y Chiu, Yinka Oyelese, Morgan R Peltier","doi":"10.1007/s43032-025-01862-2","DOIUrl":"10.1007/s43032-025-01862-2","url":null,"abstract":"<p><p>Postpartum Depression (PPD) is a common adverse pregnancy outcome, but the extent to which PPD correlates with attention deficit hyperactivity disorder (ADHD) in the offspring is unclear. Therefore, we examined if children of mothers who experienced PPD are at risk for developing ADHD and how this association may be modified by the child's sex, race/ethnicity, and gestational age at delivery. A retrospective cohort study of singleton-born children aged 3-12 years and delivered in Kaiser Permanente Southern California hospitals between 01/01/2010-12/31/2019 (n = 229,860) was performed using electronic health records. ICD- 9-CM and ICD- 10-CM codes and pharmacy records were used to ascertain exposure and outcomes of interest. Incidence rates and adjusted hazard ratios (aHR) were estimated to quantify the association between PPD and childhood ADHD. Children of women with PPD were more likely to be diagnosed with ADHD than children of women without PPD (8.85 vs. 5.18/1000 person-years, aHR = 1.76, confidence interval (CI): 1.66-1.86). Among women who delivered at 29-32 and 33-36 weeks of gestation, PPD was associated with 1.61-fold (95% CI: 1.06-2.57) and 1.72-fold (95% CI: 1.44-2.06) increased risk of ADHD, respectively, compared to pregnancies without a history of PPD. PPD was associated with an increased ADHD risk for all racial/ethnic groups and both child sex categories. PPD may help identify at-risk children who could benefit from earlier surveillance and interventions. Additional studies are needed to determine if PPD has a causal relationship with ADHD or if these conditions have a common risk factor.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1959-1967"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Dominant Assumptions to Recessive Trait: Rethinking BMP15 in Ovarian Dysfunction.","authors":"Bérénice Hervé, Camille Verebi, Marion Bonnier, Pénélope Jordan, Diana Stroe, Charlotte Voisin, Diane Hill, Cynthia Sarfati, Maud Lansiaux, Daniel Terral, Anne Bachelot, Jean Michel Dupont, Sophie Christin-Maitre, Geneviève Plu-Bureau, Thierry Bienvenu","doi":"10.1007/s43032-025-01831-9","DOIUrl":"10.1007/s43032-025-01831-9","url":null,"abstract":"<p><p>Approximately 1% of women experience premature ovarian insufficiency (POI) before the age of 40. Genetic factors play a significant role in the development of POI. The BMP15 gene is one of the first genes involved in POI. However, the pathogenicity and the penetrance of BMP15 variants still remain unclear. A cohort of 500 patients with POI/DOR (diminished ovarian reserve) underwent next generation sequencing of the entire coding region of the BMP15 gene. The frequency of each identified BMP15 variant was then compared with that of the general population, taking into account the ethnicity of each individual. Screening of the entire coding region of the BMP15 gene allowed us the identification of 11 different variants, including one pathogenic non-sense variant. A total of 54 patients with POI (10.8%) carried out at least one BMP15 variant. With regard to missense variants, we observed a significant overrepresentation of two missense variants (2/10 missense variants) in our 500 POI patients when compared to the general or specific ethnic subgroups. Of interest is the identification of one homozygous POI subject with a loss-of-function variant. The results of the segregation analysis indicated that this variant was inherited by the healthy mother (menarche at 16 years of age, and physiological menopause at 53 years of age) and by the father. This epidemiological study suggests that the majority of heterozygous missense variants could be considered as benign variants, while the homozygous loss-of-function variant could be considered as pathogenic variant. Several missense variants should be considered as variants of uncertain significance, conferring a moderate risk with probable partial and very low penetrance and/or expressivity. However, it should be noted that these variants are only deleterious in the compound heterozygous or homozygous status.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1926-1938"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Shared Gene Signatures and Immune Infiltration Characteristics Between Gestational Diabetes Mellitus and Preeclampsia by Integrated Bioinformatics Analysis and Machine Learning.","authors":"Yaqian Li, Xueqi Li, Tingting Xu, Daijuan Chen, Fan Zhou, Xiaodong Wang","doi":"10.1007/s43032-025-01847-1","DOIUrl":"10.1007/s43032-025-01847-1","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Gestational diabetes mellitus (GDM) and preeclampsia (PE) are common and serious disorders of pregnancy that threaten maternal safety and perinatal outcomes. Generally, GDM is recognized as an independent risk factor for the development of preeclampsia, while a history of preeclampsia in primiparous women is also a risk factor for GDM in subsequent pregnancies. However, the intricate underlying mechanisms of GDM and PE remain elusive. This study developed a diagnostic prediction model for GDM and PE. It investigated the correlation between shared signature genes and immune infiltration characteristics, by employing bioinformatic analysis combined with a machine learning strategy. The microarray datasets GSE103552 and GSE74341 from the Gene Expression Omnibus (GEO) database were used to obtain differentially expressed genes (DEGs). Then, signature genes were identified from the common DEGs via the methods of random forest (RF) algorithms, and artificial neural network (ANN) models. Furthermore, the immune infiltration patterns associated with GDM and PE were explored and validated in the training and testing sets. Moreover, to uncover the molecular mechanisms involved, an mRNA-miRNA network of target genes was constructed, and potential therapeutic drugs for GDM and PE were explored by querying the Connectivity Map (CMap) database. We obtained 45 DEGs by intersecting upregulated and downregulated DEGs from the GSE103552 and GSE74341 datasets. The results of GO annotation indicated that these 45 DEGs were mainly enriched in the process of cell cycle, and KEGG enrichment analysis indicated significant associations with immune signal transduction pathways and immune-related infectious disease. Six signature genes, namely TRA2A, NPM3, PHF5A, SNORD1C, PLXNA3, and C14orf142, were determined by machine learning models, and a diagnostic prediction model for GDM and PE was constructed based on these key genes, validating the highest prediction in the testing set. Moreover, we found increased infiltration of iDCs and T cell co-inhibition in the GDM group, while neutrophil, Th2 cell, and HLA levels were found to have decreased significantly. The PE group showed a significant increase in mast cells. In addition, the identified key genes were found to have potential associations with various immunocytes, immune functions, and checkpoints in the training and testing sets. Then, a miRNA-gene network analysis predicted several key miRNAs-miR-204, miR-23abc, miR-9, miR-205, and miR-455-5p-that might play significant roles in regulating these DEGs. In addition, the research also identified four potential therapeutic compounds for GDM (prima-1-met, geranylgeraniol, MLN-8054, and LY-364947), along with other drugs (deferiprone, peucedanin, MPEP, and IWR-1-endo) that could be targeted for treating PE. In summary, this work identified six signature genes (TRA2A, NPM3, PHF5A, SNORD1C, PLXNA3, and C14orf142) as potential genetic biomarkers for the diagnostic predic","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1886-1904"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Maternal Serum Uric Acid Levels and Risk of Low-Birth-Weight and Small for Gestational Age Infant: A Systematic Review and Meta-Analysis.","authors":"Khalid Khadawardi, Mohammed Abuzaid, Saeed Baradwan, Saud Owaimer Alsehaimi, Hedaya Albelwi, Seham Adel Arishi, Mooza M Alzayed, Osama Alomar, Ahmed Abu-Zaid","doi":"10.1007/s43032-025-01853-3","DOIUrl":"10.1007/s43032-025-01853-3","url":null,"abstract":"<p><p>Although the association between high uric acid levels and adverse pregnancy outcomes has been explored, the effects of elevated uric acid levels on the risk of low-birth-weight (LBW) and small for gestational age (SGA) remain unclear. Therefore, this systematic review and meta-analysis aimed to investigate the relationship between uric acid levels during pregnancy and the risk of LBW. PubMed, Scopus, Cochrane central and Web of Science databases were searched up to September 2024 for relevant observational studies. A random effects model was used to estimate pooled odds ratios (OR) and 95% confidence intervals (95% CI). To assess the heterogeneity of included studies, the I² index was used. Finally, 29 observational studies were eligible for inclusion in this meta-analysis. Pooled analysis showed that higher uric acid levels significantly increased the risk of LBW (OR = 2.28, 95% CI: 1.62-3.22, P < 0.001; I² = 93.14%, n = 21 studies) and SGA (OR = 1.77, 95% CI 1.38-2.27, P < 0.001, I² = 91.67%, n = 14 studies). However, there was no significant effect of high uric acid levels and macrosomia (OR = 1.29, 95% CI 0.52-3.18, P = 0.59, I² = 71.86%, n = 3 studies). This study demonstrated a positive association between uric acid levels and the risk of LBW and SGA. It appears that elevated uric acid can significantly impact neonatal health, highlighting the critical importance of uric acid monitoring during pregnancy.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1803-1812"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic Outcomes, Live-Birth Outcomes After Embryo Transfer, and Euploid Rates for Various Direct Cleavage Timing Definitions During First Cytokinesis: A Single-Center, Large-Cohort, Retrospective Study.","authors":"Satoshi Ueno, Shin Katakura, Tadashi Okimura, Keiichi Kato","doi":"10.1007/s43032-025-01842-6","DOIUrl":"10.1007/s43032-025-01842-6","url":null,"abstract":"<p><p>Although essential to confirm the clinical utility of direct cleavage embryos, the timing of rapid cleavage (RpiC) has not been specifically defined. This study aimed to explore the differences in embryonic and clinical outcomes based on varying timing parameters of direct cleavage during first cytokinesis, using time to reach the three-cell stage (t3) minus time to reach the two-cell stage (t2). We analyzed 19,796 fertilized embryos (in 6,907 patients) from a single center between September 2019 and December 2020. The embryos were cultured using EmbryoScope, and t2 and t3 were recorded. Trichotomous mitosis (TM) was defined as t3 - t2 = 0 h, and RpiC events were divided into four groups, as follows: 0 h < t3 - t2 < 1 h (RpiC-1), 1 h ≤ t3 - t2 < 3 h (RpiC-3), 3 h ≤ t3 - t2 < 5 h (RpiC-5), and 5 h ≤ t3 - t2 < 7 h (RpiC-7). Additionally, 7 h ≤ t3 - t2 < 14 h was defined as normal cleavage. After single-cleavage embryo transfer, the live-birth, TM, and RpiC-1 rates were significantly lower than those in other groups. Similarly, when blastocysts were utilized, the TM and RpiC-1 rates were significantly lower than those in other groups. This study suggests that embryos with TM or RpiC-1 (t3 - t2 < 1 h) should be cultured to the blastocyst stage to prevent unnecessary embryo transfers, although outcomes may vary in different scenarios, i.e., by institution and patient.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1876-1885"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Antidepressants on Male Fertility and Seminal Parameters: A Systematic Review.","authors":"Lorena Oliveira de Paula Dos Santos, Paula Rafaela Queiroz da Silva, Nicoly Caixêta Gonçalves, Hugo Felix Perini, Gláucia Eloisa Munhoz de Lion Siervo","doi":"10.1007/s43032-025-01858-y","DOIUrl":"10.1007/s43032-025-01858-y","url":null,"abstract":"<p><p>Depressive disorders affect a significant number of individuals worldwide, including men of reproductive age. The impact of antidepressant treatment on seminal and fertility parameters remains inconclusive, with limited clinical studies available on this topic. This systematic review aimed to compile the existing literature on the influence of various antidepressants on sperm and fertility-related parameters in men. In April 2023, a bibliographic search was conducted using the PubMed/Medline, Web of Science, and Scopus databases. Search terms included\"fertility,\"\"sperm,\"and\"semen,\"combined with terms related to various antidepressants and their respective drugs. Clinical studies investigating fertility and/or semen parameters following antidepressant treatment in men were included. Studies outside the scope of this review were excluded. Data collected included details of the intervention and outcomes-such as the class of antidepressant, specific drug, dosage, duration of treatment, and age of subjects-along with data on sperm analysis and fertility-related parameters. This review included 5 articles focused on human studies. Selective serotonin reuptake inhibitors (SSRIs) are the most studied class of antidepressants. Findings on sperm-related impairment are conflicting. The available human studies are limited and often involve short-term treatment regimens, which may not accurately reflect the effects of long-term exposure. This work highlights the significant gap in reliable evidence regarding the effects of antidepressant drugs on seminal and fertility parameters in men of reproductive age.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1768-1774"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carvacrol Shows Potential as a Treatment for Endometriosis with Inflammatory Reaction and Antioxidant Properties, Demonstrated in both In Vivo and In Vitro Studies.","authors":"Hyewon Jang, Wonhyoung Park, Hee Seung Kim, Gwonhwa Song, Whasun Lim, Sunwoo Park","doi":"10.1007/s43032-025-01866-y","DOIUrl":"10.1007/s43032-025-01866-y","url":null,"abstract":"<p><p>Carvacrol is a phenolic monoterpenoid found in essential oils of oregano, thyme, and other plants. It possesses pharmacological activities, including antioxidant, anticancer, and anti-inflammatory properties, against several diseases. However, research on the treatment effect of endometriosis has not yet been conducted. Endometriosis is a condition characterized by tissues resembling the endometrium beyond the confines of the uterus, leading to intense pelvic pain and heightened risks of infertility. This study aimed to confirm the effectiveness of carvacrol as a treatment strategy for endometriosis with inflammatory regulation and antioxidant property. In the in vivo study, C57BL/6 J mice were orally administered 100 mg/kg carvacrol for 4 weeks and confirmed a decreased size of endometriosis lesions in the carvacrol-treated group, increased number of T cells in the spleen, and increased expression of cytokine-related genes in both tissues. Immortalized human ovarian endometriotic stromal cells (ihOESCs), derived from endometriotic lesions in patients, were used as an in vitro model for studying endometriosis pathophysiology. The findings of the in vitro study confirmed that carvacrol regulates calcium homeostasis, induces antioxidant effects, increases inflammatory response, deactivates the phosphatidylinositol 3-kinase (PI3K) signaling pathway, and ultimately induces autophagy. Therefore, we determined that carvacrol is suitable as an anti-inflammatory and anti-oxidant treatment strategy for endometriosis.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1825-1839"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}