Reproductive Sciences最新文献

{"title":"Assisted Reproductive Technology as Predictor for Preeclampsia: A Systematic Review of Current Clinical Prediction Models.","authors":"Géraldine C M Lafeber, Marie-Louise P van der Hoorn, Saskia le Cessie, Eileen E L O Lashley","doi":"10.1007/s43032-026-02168-7","DOIUrl":"10.1007/s43032-026-02168-7","url":null,"abstract":"<p><p>Assisted Reproductive Technology (ART) increases the risk of preeclampsia (PE), yet no specific prediction model tailored to the ART population exists. This review evaluates prediction models incorporating ART as a predictor, focusing on risk of bias, performance, generalizability and clinical applicability. A systematic MEDLINE search up to July 2023 identified models predicting PE that included ART as a predictor. Exclusion comprised studies on outcomes after PE onset, univariable models and validation studies of previously validated models. Risk of bias, applicability and generalizability were assessed using the Prediction model Risk Of Bias ASsessment Tool (PROBAST) and the Transparent Reporting of a multivariable prediction model for Individual Prognosis and Diagnosis (TRIPOD) guideline. Out of 15,305 records, 161 articles were assessed in full-text, yielding fifteen eligible studies. Eleven studies were added from a prior systematic review. Performance varied widely (AUC 0.58-0.99) and only few (7/26) models had been externally validated. Risk of bias was unclear in most studies (21/26), due to analytical issues. Despite ART being an established risk factor for PE, robust and generalizable models with proven external validity are lacking. Future research should prioritize development of a high quality prediction model including ART, or a model developed specifically within ART populations.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1907-1930"},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13645924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Perineal Massage and Warm Compresses Technique in Postpartum Perineal Pain. A Secondary Analysis from a Randomised Controlled Trial.","authors":"Silvia Rodrigues, Paulo Silva, Margarida Esperança, João Neves Silva, Ramon Escuriet","doi":"10.1007/s43032-026-02204-6","DOIUrl":"10.1007/s43032-026-02204-6","url":null,"abstract":"<p><p>Postpartum perineal pain can lead to failure to fulfill maternal role. Evaluate the effect of perineal massage and warm compresses technique versus hands-on technique during the second stage of labor on postpartum perineal pain. A single-centre, prospective, randomized controlled trial was conducted between March 1st, 2019, and December 31st, 2020, at Hospital of X. Women with 18 years or older, between 37 and 41 weeks pregnant, in whom a vaginal birth of a fetus in the cephalic presentation was planned were recruited. Of the 848 women recruited to randomised controlled trial to prevent perineal trauma, 800 fulfilled the inclusion criteria (perineal massage and warm compresses group, n = 400 and control group, n = 400), all were included in secondary analysis at 24 h and 498 women were included at 3 and 6 months after birth. In the perineal massage and warm compresses group, women received perineal massage and warm compresses and in the control group, women received hands-on technique. Application of Numerical Rating Scale at 24 h, 3 and 6 months after birth. Women in the perineal massage and warm compresses group reported lower perineal pain at 24 h, 3 and 6 months after birth, compared to hands-on technique group but the difference was not statistically significant between the groups. The occurrence of obstetric anal sphincter injury and episiotomy were the main determinants for perineal pain at 24 h, 3 and 6 months postpartum. The perineal massage and warm compresses technique was not associated with more postpartum perineal pain. TRIAL REGISTRATION: http://www.ClinicalTrials.gov:NCT05854888.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1999-2009"},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Role of ALPK2 in Endometrial Cancer Progression: From Biological Function to Clinical Translation.","authors":"Juan Hu, Anquan Shang, Hui Wang, Lei Han, Lei Geng, Yongchang Miao, Chao Lu","doi":"10.1007/s43032-026-02109-4","DOIUrl":"10.1007/s43032-026-02109-4","url":null,"abstract":"<p><p>Given the limitations of traditional indicators in evaluating the prognosis of endometrial cancer, this study identified the core genes related to prognosis, diagnosis, and treatment within the alpha-protein kinase (ALPK) family to address the lack of research on ALPK family genes in endometrial cancer. Using data from the TCGA database, we combined bioinformatics analyses (Cox regression, Kaplan-Meier analysis, GSVA enrichment, KEGG pathway analysis) and cell experiments (constructing ALPK2 knockdown/overexpression models; conducting clone formation assays; CCK-8 and other experiments), analysis of the associations among gene expression, prognosis, clinicopathological characteristics, and drug sensitivity was conducted. Only ALPK2 was identified as an independent risk factor for poor prognosis of endometrial cancer (HR > 1, P < 0.05). It was highly expressed in tumor tissues (P < 0.01) and associated with high-risk characteristics such as serous subtype and stage III/IV (P < 0.001). ALPK2 promoted tumor progression by regulating core processes such as proliferation and invasion, and pathways including ECM-receptor interaction and PI3K-Akt signaling. Its high expression was associated with resistance to cisplatin and paclitaxel, as well as sensitivity to olaparib (P < 0.01). Moreover, it demonstrated good diagnostic efficacy for stage III with an AUC of 0.872 and showed broad applicability. ALPK2 is a potential key molecule for prognostic assessment, diagnostic accuracy, and targeted therapy of endometrial cancer, providing a new basis for optimizing the diagnosis and treatment of this disease.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2037-2052"},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of 7-ketocholesterol in Placental Pathophysiology: Implications for Fetal Programming and Long-term Health: A Narrative Review.","authors":"Nila Ganamurali, Sarvesh Sabarathinam","doi":"10.1007/s43032-026-02207-3","DOIUrl":"10.1007/s43032-026-02207-3","url":null,"abstract":"<p><p>7-Ketocholesterol (7-KC), a major cholesterol oxidation product, is increasingly recognized as a potent mediator of oxidative stress, inflammation, and cellular dysfunction. While its roles in cardiovascular and neurodegenerative diseases are well established, its significance at the maternal-fetal interface remains poorly understood. This narrative review summarizes current evidence on the sources, placental transport, metabolism, and biological effects of 7-KC during pregnancy. Emerging studies suggest that 7-KC may impair trophoblast function, disrupt placental homeostasis, and influence fetal developmental pathways. Within the Developmental Origins of Health and Disease (DOHaD) framework, 7-KC represents a potential mediator linking adverse intrauterine environments to long-term offspring health outcomes, highlighting important directions for future research.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1991-1998"},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRIP10 as a Key Regulator in Fetal Growth Restriction: a Study Integrating Bioinformatics Screening with Experimental Validation.","authors":"Xinjun Li, Shuo Xu, Xiangnan Zhang, Wenning Lian, Guangpu Liu, Shengpu Wang","doi":"10.1007/s43032-026-02222-4","DOIUrl":"https://doi.org/10.1007/s43032-026-02222-4","url":null,"abstract":"<p><p>Fetal growth restriction (FGR) is a leading cause of perinatal mortality, yet its molecular mechanisms remain poorly understood. This study integrated bioinformatic analysis with experimental validation to identify key genes involved in FGR pathogenesis. Transcriptome datasets GSE114691 and GSE24129 from the GEO database were analyzed, revealing 5,788 differentially expressed genes (1,450 upregulated, 4,338 downregulated) between FGR and control placental samples. Functional enrichment analysis indicated that downregulated genes were primarily involved in mitochondrial function, aerobic respiration, and amino acid metabolism, while upregulated genes were associated with cell adhesion and histone modification. Weighted gene co-expression network analysis (WGCNA) identified 19 modules, with the darkorange module showing the strongest positive correlation with FGR. LASSO regression further selected eight candidate genes, among which only TRIP10 demonstrated consistent and significant upregulation in the independent validation dataset and exhibited excellent diagnostic performance (AUC = 0.906). Western blot confirmed significant upregulation of TRIP10 protein in FGR placental tissues. Functional assays in JEG-3 trophoblast cells revealed that TRIP10 knockdown significantly enhanced cell migration and invasion, while TRIP10 overexpression suppressed these processes. Collectively, this study identifies TRIP10 as a key gene significantly upregulated in FGR placentas and establishes TRIP10 as a negative regulator of trophoblast migration and invasion, suggesting that TRIP10 dysregulation may contribute to impaired placental development and FGR pathogenesis, representing a promising biomarker and potential therapeutic target.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Longitudinal Profiling of IgG and IgA Glycosylation in Pregnancy Reveals Early Associations with BMI, Nulliparity and Conception Mode: The Rotterdam Periconception Cohort.","authors":"Lotte W Voskamp, Anna Daniels, Melek Rousian, Mandy van Hoek, Manfred Wuhrer, Régine P M Steegers-Theunissen, A H Jan Danser, Koen Verdonk","doi":"10.1007/s43032-026-02227-z","DOIUrl":"10.1007/s43032-026-02227-z","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kisspeptin-10 Alleviates Obesity-induced Testicular Oxidative Stress and Mitochondrial Dysfunction via Modulation of Mitophagy and Autophagy Signaling.","authors":"Gözde Arkalı, Mehmet Çay, Edanur Güler Ekmen, Tutku Can Acısu, Mustafa Fi̇ri̇k, Abdullah Toz, Abdurrauf Yüce, Mesut Aksakal","doi":"10.1007/s43032-026-02211-7","DOIUrl":"https://doi.org/10.1007/s43032-026-02211-7","url":null,"abstract":"<p><p>Obesity impairs male reproductive function via oxidative stress and mitochondrial dysfunction. Kisspeptin-10 may regulate redox balance and mitochondrial homeostasis, but its role in obesity-related testicular dysfunction remains unclear. This study aimed to investigate whether Kisspeptin-10 ameliorates obesity-induced testicular oxidative stress and sperm dysfunction, with particular emphasis on mitophagy, autophagy, and apoptosis-related pathways. Forty male Sprague Dawley rats were divided into four groups: Control, Obesity, Kisspeptin-10, and Obesity+Kisspeptin-10. Obesity was induced by feeding rats a high-fat diet (60% kcal from fat) for 12 weeks. At the end of week 12, rats with a Lee index ≥ 300 were considered obese. During the subsequent 4-week treatment period, the Obesity and Obesity+Kisspeptin-10 groups continued to receive the high-fat diet, whereas the Control and Kisspeptin-10 groups continued to receive the standard diet. Kisspeptin-10 (50 nmol/kg, i.p.) was administered to both the Kisspeptin-10 and Obesity+Kisspeptin-10 groups from week 13 to week 16. Oxidative stress markers in the testes, sperm parameters, sperm mitochondrial membrane potential (MMP), and sperm deoxyribonucleic acid (DNA) integrity were assessed; in addition, protein expression levels of markers for mitophagy, autophagy, and apoptosis were evaluated. Obesity impaired antioxidant capacity, sperm quality, mitochondrial function, and reproductive organ weight, while increasing lipid peroxidation and sperm abnormalities. Kisspeptin-10 reduced oxidative stress by lowering malondialdehyde (MDA) and increasing glutathione (GSH) levels, improved sperm motility and concentration, reduced tail abnormal sperm ratio, showed a non-significant tendency toward partial restoration of mitochondrial function, decreased DNA damage, and increased prostate weight. It was also associated with increased expression of the mitophagy-related proteins PTEN-induced putative kinase 1 (PINK1) and Prohibitin 2 (PHB2), and increased Beclin-1 expression, with no significant effects on Parkin, Microtubule-associated protein 1 light chain 3-II (LC3-II), or apoptosis-related proteins. Kisspeptin-10 may alleviate obesity-induced testicular dysfunction, potentially through modulation of redox balance and mitochondrial quality-control mechanisms, including changes in mitophagy- and autophagy-related protein expression. Although these findings suggest that Kisspeptin-10 is a promising candidate for the treatment of obesity-related male infertility, its effects on downstream autophagy-related signaling and apoptosis appear to be limited.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of Embryo Transfer Following Laser-assisted Hatching in Patients with Recurrent Implantation Failure: A Systematic Review and Meta-analysis of Randomized Controlled Trials.","authors":"Mohammadamin Parsaei, Elaheh Karimi, Parsa Seifi, Masoumeh Dehghan Tarzjani, Azadeh Tarafdari","doi":"10.1007/s43032-026-02219-z","DOIUrl":"https://doi.org/10.1007/s43032-026-02219-z","url":null,"abstract":"<p><p>Recurrent implantation failure (RIF) affects approximately 10% of women undergoing embryo transfer. While laser-assisted hatching (LAH) has been proposed to improve outcomes, its efficacy remains uncertain in RIF patients. This study evaluated whether LAH improves outcomes for women with RIF. A systematic review was conducted in PubMed, Web of Science, Scopus, and Embase on January 22, 2025, for randomized controlled trials comparing embryo transfer outcomes following LAH versus controls in RIF. Random-effects meta-analyses generated log odds ratios (LOR), with evidence certainty graded using the Grading of Recommendations Assessment, Development and Evaluation tool. Nine studies involving 2,481 women with RIF were included. Meta-analysis revealed significantly higher rates in the LAH group compared to controls for clinical pregnancy (LOR = 0.643 [0.267, 1.019]; I² = 70.62%, very low certainty), ongoing pregnancy (LOR = 0.811 [0.076, 1.546]; I² = 63.54%, very low certainty), and live birth rate (LOR = 0.519 [0.127, 0.911]; I² = 15.21%, moderate certainty). Additionally, the LAH group had a significantly lower miscarriage rate (LOR = -0.632 [-1.136, -0.127]; I² = 0.00%, moderate certainty). No significant differences were observed for implantation rates (LOR = 0.691 [-0.036, 1.417]; I² = 87.14%, very low certainty) or multiple pregnancy rates per clinical pregnancy (LOR = 0.166 [-0.269, 0.600]; I² = 0.00%, moderate certainty). Our findings suggest that LAH before embryo transfer may improve pregnancy and live birth rates following embryo transfer in women with RIF; however, no significant effect was observed on implantation. Due to observed heterogeneity and the low quality of available studies, high-quality, large-scale randomized controlled trials are needed to confirm these results.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Integrated Metabolomic and Transcriptomic Profiling Reveals a Distinct Pathological Aging State in Diminished Ovarian Reserve of Advanced Reproductive-Age Women.","authors":"Zhengda Li, Shikai Wang, Xianbao Mao, Xiaohui Zhang, Pingpin Wei, Liangshi Chen, Lintao Xue, Ziyun Ruan","doi":"10.1007/s43032-026-02213-5","DOIUrl":"https://doi.org/10.1007/s43032-026-02213-5","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible Involvement of miR-26a-3p in Regulating Cyclin D1(CCND1) in the Pathogenesis of Premature Ovarian Insufficiency (POI).","authors":"Erqiu Du, Jin Pan, Ling Zhang, Wei Xu","doi":"10.1007/s43032-026-02218-0","DOIUrl":"https://doi.org/10.1007/s43032-026-02218-0","url":null,"abstract":"<p><p>Premature ovarian insufficiency (POI) affects female fertility; however, the mechanisms are not yet fully understood. The aim of this study was to investigate the relationships among bisphenol A (BPA), microRNAs (miRNAs), and the cell cycle-related protein Cyclin D1 (CCND1) in ovarian granulosa cells. Using cell culture and transfection techniques, we analysed the effects of BPA on the proliferation of ovarian granulosa cells. Concurrently, qRT‒PCR and Western blotting were employed to assess miRNA expression levels and CCND1 protein levels, respectively. Our results demonstrated that BPA significantly inhibited the proliferation of ovarian granulosa cells. This inhibition was closely associated with the upregulation of specific miRNA expression and the downregulation of CCND1 protein expression. Furthermore, the overexpression of the identified miRNAs partially reversed the BPA-induced changes in CCND1 expression. These findings reveal a potential role for BPA in POI pathogenesis through the regulation of miRNAs and the cell cycle protein CCND1, providing a novel perspective on the mechanisms underlying this disorder. Future research should further elucidate the specific roles of these miRNAs and CCND1 in ovarian insufficiency and explore their potential application as therapeutic targets.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}