Reproductive Sciences最新文献

{"title":"Increased Endometrial CD38 and CD138 Positivity in Women with Ectopic Pregnancy History: A Retrospective Study.","authors":"Yangzhi Li, Yang Li, Dongmei Chen, Xiaoqian Xie, Chao Zhang, Xiaozhu Zhong, Meiqing Xie","doi":"10.1007/s43032-026-02246-w","DOIUrl":"https://doi.org/10.1007/s43032-026-02246-w","url":null,"abstract":"<p><p>Ectopic pregnancy (EP) represents the most common gynecological emergency. However, its specific etiology and pathogenesis remain incompletely understood. This retrospective study investigated the correlation between chronic endometritis (CE) markers (CD38 and CD138) in the endometrium and EP. Patients were categorized as follows: women with a history of EP but no prior intrauterine operations (EP Group A; n = 98), women with a history of EP and at least one intrauterine operation (EP Group B; n = 244), and a control group of women with recurrent spontaneous abortion (RSA; n = 267). The prevalence of CD38 and/or CD138 positive cells was 54.1% (53/98) in Group A, 72.5% (177/244) in Group B, and 28.1% (75/267) in the RSA group. Our findings demonstrate a significantly higher rate of CD38 and/or CD138 positivity in the endometrium of women with a history of EP compared to the RSA controls. This result suggests that chronic endometritis may be an underlying pathogenic factor contributing to ectopic pregnancy.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles and Biological Effects of Polysaccharides in Polycystic Ovary Syndrome: A Narrative Review.","authors":"Ning Ding, Chang Sun, Haotian Wang, Yingchun Lv, Yanhua Han, Hongying Kuang","doi":"10.1007/s43032-026-02232-2","DOIUrl":"https://doi.org/10.1007/s43032-026-02232-2","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) represents a prevalent and clinically significant reproductive-endocrine disorder among women of childbearing age. While dietary interventions, regular physical activity, and targeted lifestyle modifications have been validated to confer therapeutic benefits for PCOS management, polysaccharides key bioactive components of higher plants with extensive distribution throughout the plant and animal kingdoms exhibit distinct pleiotropic bioactivities, including anti-inflammatory, immunomodulatory, hypoglycemic, lipid-lowering, and anti-obesogenic properties. In the context of PCOS treatment, these natural macromolecules hold considerable promise for alleviating clinical manifestations, ameliorating insulin resistance and obesity, attenuating inflammatory responses, and restoring sex hormone homeostasis. Cumulative evidence from preclinical and clinical studies has demonstrated that polysaccharides can improve menstrual irregularities, reduce body weight, lower blood glucose and serum testosterone levels, and suppress aberrant inflammation. The present review systematically summarizes the multifaceted effects of diverse polysaccharide compounds on PCOS, aiming to provide insights into their potential as complementary therapeutic agents for this complex disorder.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Exposure to Green Light-emitting Diodes Alleviates Endometriosis-associated Pain in Mice with Induced Deep Endometriosis.","authors":"Jing Dong, Xishi Liu, Yuedong Wang, Sun-Wei Guo","doi":"10.1007/s43032-026-02250-0","DOIUrl":"https://doi.org/10.1007/s43032-026-02250-0","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149195410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping Hydrogen Sulfide Biosynthesis and Oxidation Pathways in Placental Health and Disease: a Scoping Review.","authors":"Anord Nkuba, Caroline J Bushell, Leni R Rivera, Kathryn Aston-Mourney, Malik Q Mahmood, Bryony A McNeill","doi":"10.1007/s43032-026-02236-y","DOIUrl":"https://doi.org/10.1007/s43032-026-02236-y","url":null,"abstract":"<p><p>Hydrogen sulfide (H₂S) plays an important role in placental function. Its bioavailability depends on the balance between enzymatic synthesis by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE/CTH) and 3-mercaptopyruvate sulfurtransferase (3-MST/MPST), and mitochondrial oxidation through sulfide:quinone oxidoreductase (SQOR), persulfide dioxygenase (ETHE1) and thiosulfate sulfurtransferase (TST). However, the extent to which H₂S synthesis, oxidation and experimental modulation have been investigated in placental systems remains unclear. This scoping review mapped the placental H₂S literature to identify where evidence is concentrated and where gaps remain. Following JBI methodology and PRISMA-ScR reporting, Scopus, Embase, MEDLINE and Web of Science were searched for primary studies involving placental or trophoblast H₂S biology. Sixty-four studies were included. Pre-eclampsia dominated the literature (n = 27), while most other pregnancy conditions were sparsely represented. CBS and CSE/CTH were investigated in 34 and 29 studies respectively, while 3-MST/MPST was investigated in only seven studies. None of the included studies assessed the principal mitochondrial sulfide-oxidation enzymes SQOR, ETHE1 or TST. Sulfide concentrations, relative assay signals or H₂S production were reported in 23 studies across six analytical approaches. Sulfide measurement was highly heterogeneous across studies, making direct comparison between studies limited. H₂S was experimentally modulated in 85 intervention applications but measured in only 23. Overall, placental H₂S research is concentrated on biosynthetic enzyme expression and pre-eclampsia, while sulfide clearance, early gestation and standardised measurement remain major gaps. These gaps limit interpretation of changes in placental H₂S bioavailability and the evaluation of donor-based therapeutic strategies.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Endoplasmic Reticulum Stress in Pregnant Women with Hyperemesis Gravidarum.","authors":"Bahriye Oznur Oguçbilek, Kenan Kuzu, Fatma Akat, Eissa Almaghrebi, Husamettin Vatansev, Sevcan Sarıkaya","doi":"10.1007/s43032-026-02228-y","DOIUrl":"https://doi.org/10.1007/s43032-026-02228-y","url":null,"abstract":"<p><p>The role of the endoplasmic reticulum (ER) stress response in hyperemesis gravidarum (HG) remains incompletely understood. This study evaluated circulating PERK and ATF-4 levels and their associations with clinical and laboratory characteristics in HG. This prospective case-control study, conducted from August to November 2024, included 85 pregnant women with HG within the first 16 weeks of gestation and 85 healthy pregnant controls. Serum PERK and ATF-4 levels were measured using ELISA. Group comparisons, Spearman correlation analyses, and receiver operating characteristic (ROC) analysis were performed. Serum ATF-4 levels were significantly lower in the HG group than in controls [4.55 (0.82-42.86) vs. 6.16 (2.23-29.27) ng/mL; p = 0.002], whereas PERK levels did not differ between groups [3.73 (0.16-15.37) vs. 3.29 (0.67-19.36) ng/mL; p = 0.965]. Strong positive correlations were observed between ATF-4 and PERK in the HG group (ρ = 0.837), control group (ρ = 0.709), and overall study population (ρ = 0.765) (all p < 0.001). Within the HG group, ATF-4 levels did not differ significantly according to urinary ketone positivity (p = 0.282). ATF-4 showed limited discriminatory performance for HG (AUC = 0.638; p = 0.001; sensitivity 45.9%; specificity 77.6%). HG is associated with lower circulating ATF-4 levels without a corresponding change in PERK. These findings support further molecular and longitudinal investigation of the PERK-ATF-4 axis in HG.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Endoplasmic Reticulum Stress in Pregnant Women with Hyperemesis Gravidarum.","authors":"Bahriye Oznur Oguçbilek, Kenan Kuzu, Fatma Akat, Eissa Almaghrebi, Hüsamettin Vatansev, Sevcan Sarıkaya","doi":"10.1007/s43032-026-02233-1","DOIUrl":"https://doi.org/10.1007/s43032-026-02233-1","url":null,"abstract":"<p><p>The role of the endoplasmic reticulum (ER) stress response in hyperemesis gravidarum (HG) remains incompletely understood. This study evaluated circulating PERK and ATF-4 levels and their associations with clinical and laboratory characteristics in HG. This prospective case-control study, conducted from August to November 2024, included 85 pregnant women with HG within the first 16 weeks of gestation and 85 healthy pregnant controls. Serum PERK and ATF-4 levels were measured using ELISA. Group comparisons, Spearman correlation analyses, and receiver operating characteristic (ROC) analysis were performed. Serum ATF-4 levels were significantly lower in the HG group than in controls [4.55 (0.82-42.86) vs. 6.16 (2.23-29.27) ng/mL; p = 0.002], whereas PERK levels did not differ between groups [3.73 (0.16-15.37) vs. 3.29 (0.67-19.36) ng/mL; p = 0.965]. Strong positive correlations were observed between ATF-4 and PERK in the HG group (ρ = 0.837), control group (ρ = 0.709), and overall study population (ρ = 0.765) (all p < 0.001). Within the HG group, ATF-4 levels did not differ significantly according to urinary ketone positivity (p = 0.282). ATF-4 showed limited discriminatory performance for HG (AUC = 0.638; p = 0.001; sensitivity 45.9%; specificity 77.6%). HG is associated with lower circulating ATF-4 levels without a corresponding change in PERK. These findings support further molecular and longitudinal investigation of the PERK-ATF-4 axis in HG.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Telomere Related Senescence-Genes in Cervical Cancer.","authors":"Cong Xu, Yonghong Xu, Qingcao, Guoling Luo, Jingwen Yu, Huaqiu Chen, Guangming Wang","doi":"10.1007/s43032-026-02217-1","DOIUrl":"https://doi.org/10.1007/s43032-026-02217-1","url":null,"abstract":"<p><p>Globally, cervical cancer is a prevalent cancer among women and ranks as the fourth leading cause of mortality in gynecological cancers. However, how telomeres affect cervical cancer remains uncertain. The research entailed the procurement of telomere-associated genes (TRGs) from TelNet. The Cancer Genome Atlas (TCGA) database provided the clinical data and TRG expression rates in patients with cervical cancer. Within the TCGA-CESC dataset, 327 TRGs were identified, differentiating between cancerous and healthy tissues. Subsequently, we combined genes linked to aging and telomeres, leading to 31 overlapping genes and performing an analysis of functional enrichment on these essential genes. Genes unique to telomeres and closely associated with cervical cancer play various roles, mainly in the cell cycle, DNA replication, and DNA repair processes. Key genes, such as those associated with cell aging, homologous recombination, and Human T-cellleukemia virus 1 infection, are vital in the life cycle of a cell. Dysfunction in these genes could lead to irregularities in cellular synthesis and apoptosis processes, potentially accelerating the progression of cervical cancer. Subsequently, the data was analyzed sequentially using single-factor Cox regression, Lasso regression, and multi-factor Cox regression techniques, leading to the development of the TRGs risk model.Among the pinpointed TCGA group (p < 0.001), cervical cancer sufferers with high-risk TRGs showed poorer outcomes. Furthermore, the TRGs risk score emerged as an independent risk factor for cervical cancer. Additionally, communities susceptible to TRGs could benefit from specific medical interventions. To conclude, our team developed a genetic risk model associated with telomeres to predict outcomes in cervical cancer patients, potentially assisting in selecting suitable treatment drugs for them.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mouse Bone Mesenchymal Stem Cells Attenuate Apoptosis by Regulating the Bcl2/Caspase3 Signaling Pathway in Premature Ovarian Failure.","authors":"Baohua Jiang, Ke Li, Li Li Zhang, Sha Sha Zhang, Jian Jun Wang, Lei Jin","doi":"10.1007/s43032-026-02094-8","DOIUrl":"https://doi.org/10.1007/s43032-026-02094-8","url":null,"abstract":"<p><p>Premature ovarian failure (POF) refers to ovarian failure and its occurrence and development are closely related to the apoptosis signaling pathway. However, the mechanism of bone mesenchymal stem cells (BMSCs) affect the regulation of apoptosis in POF has not been fully elucidated. The GSE128240 microarray dataset were downloaded from the Gene Expression Omnibus. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) databases were used to analyse the functional and pathway enrichment of genes in the most important modules. A mouse model of POF induced by cyclophosphamide (CTX) treatment was established. POF model mice were treated with mBMSCs for 4 weeks. Ovarian failure was evaluated by haematoxylin-eosin (HE) staining, the levels of oestradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH), and the number of follicles. Western blotting and immunohistochemical methods were used to measure the levels of Bcl-2, Bax and Caspase-3 proteins in the ovarian tissue of mice. Total of 1816 differentially expressed genes (DEGs) identified. The PI3K-Akt signaling pathway and ovarian steroidogenesis were functionally enriched through GO enrichment and KEGG analyses. Furthermore, the number of follicles and E2 concentration were significantly increased, and the FSH and LH concentrations were significantly decreased in CTX-induced mice treated with mBMSCs. mBMSCs attenuated apoptosis in CTX-induced mouse ovaries. Levels of Bax and Caspase-3 proteins were significantly decreased in the ovarian tissue of CTX-treated mice and significantly increased after mBMSCs treatment. Thus, mBMSCs attenuated apoptosis in CTX-induced mouse ovaries by regulating the Bcl2/Caspase3 signaling pathway, effectively treating POF.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometriosis and Adenomyosis: Mechanotransduction, Microenvironment, and Mechanisms Beyond Lesions.","authors":"Hiroshi Kobayashi","doi":"10.1007/s43032-026-02237-x","DOIUrl":"https://doi.org/10.1007/s43032-026-02237-x","url":null,"abstract":"<p><p>Endometriosis and adenomyosis are characterized by the presence of endometrial-like tissue outside its normal anatomical location and are associated with pain, infertility, and other clinical manifestations. Although they share pathogenic features, including inflammation, hormonal responses, immune dysregulation, and fibrosis, their clinical and histopathological phenotypes are highly heterogeneous. This review integrates tissue mechanics and mechanobiology with inflammation, hormonal signaling, oxidative stress, angiogenesis, and neurogenesis to propose a microenvironment-dependent framework for understanding lesion formation, maintenance, and progression. Direct measurements of tissue stiffness, including elastography, have been reported, but standardized comparisons among lesion types remain limited. Existing evidence suggests that tissue stiffening may affect cellular proliferation, invasion, fibrosis, and tissue remodeling through mechanotransduction pathways such as integrin signaling. However, much of this evidence derives from in vitro studies, experimental models, or other fibrotic diseases, and direct causal relationships in human endometriosis and adenomyosis remain insufficiently established. Conversely, chronic inflammation, recurrent bleeding, and ECM accumulation may contribute to both tissue stiffening and fibrosis, indicating reciprocal interactions between mechanical and biological processes. Thus, lesion phenotypes may reflect interactions among lesion origin, cell-intrinsic properties, and the local microenvironment. Mechanobiology may complement established inflammatory, hormonal, immune, and fibrotic concepts and provide hypotheses for understanding lesion heterogeneity and progression. Longitudinal, lesion-level studies integrating mechanical and biological parameters are needed to test these hypotheses.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149148632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLD-Based Antiretroviral Therapy Compromises Ovarian Function and Reserve: Evidence from Experimental and Clinical Studies.","authors":"Martha Ugwu, Airat A Bakare, Tawakalitu Nafiu, Olufunke O Dosumu, Edidiong N Akang","doi":"10.1007/s43032-026-02229-x","DOIUrl":"https://doi.org/10.1007/s43032-026-02229-x","url":null,"abstract":"<p><p>Combination antiretroviral therapy (cART) has improved human immunodeficiency virus (HIV) management, but long-term use is associated with reproductive dysfunction. This study investigated TLD (Tenofovir, Lamivudine and Dolutegravir)-based cART effects on ovarian function using animal models and exploratory clinical investigations in women living with HIV. In the animal study, female Wistar rats were randomized into control, TLD-based cART (22 mg/kg), and dolutegravir alone (2.5 mg/kg) groups, administered orally for 28 days. Estrous cycle, histology, oxidative stress, reproductive hormones, and ova count were assessed. Both TLD-based cART and dolutegravir significantly prolonged diestrus (p = 0.0083 and p = 0.0007, respectively) and reduced metestrus (p = 0.0057 and p = 0.0083, respectively). Treated groups showed significantly reduced ova counts (p = 0.0002 and 0.0003, respectively). Histology revealed structural alterations of the ovary, including atretic follicles, distorted stroma, and sparse theca cells. Additionally, treated groups showed increased malondialdehyde (p = 0.0421) and decreased glutathione (p = 0.0046) and catalase activities (p = 0.0411), indicating oxidative stress. Serum follicle-stimulating and luteinizing hormones were significantly reduced in both treated groups (FSH: p < 0.0001 and p = 0.0002; LH: p < 0.0001 and p = 0.0108, respectively). In the clinical investigation, serum anti-Müllerian hormone (AMH) levels were evaluated in HIV-positive women (n = 20) receiving TLD-based cART. Ten participants (50%) had AMH in low/very low categories ( < 1.0 ng/mL), while 11 (55%) had AMH below the normal reference range ( < 1.5 ng/mL). Menstrual interval and dysmenorrhea showed no significant changes. These preliminary findings suggest Dolutegravir and TLD-based cART may negatively affect female reproductive function by altering ovarian structure, hormonal balance, and ovarian reserve, potentially predisposing women living with HIV to fertility complications.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}