Reproductive Sciences最新文献

{"title":"Optimal Exercise Intensity for Enhancing Sperm Parameters: A Network Meta-Analysis of Rodent Studies.","authors":"Yinchu Hu, Ruiqiong Li, Mimi Chen, Yidong Chen, Qi Wang, Xiaowei Zhang, Yanhui Lu, Mingjie Yao, Yao Dang, Lihua Ren","doi":"10.1007/s43032-025-01926-3","DOIUrl":"https://doi.org/10.1007/s43032-025-01926-3","url":null,"abstract":"<p><p>Exercise training as a potential intervention to improve male reproductive health, its efficacy appears to be contingent on exercise intensity. However, the optimal training intensity remains poorly defined. Hence, this study aimed to identify the optimal exercise intensity for improving sperm parameters through network meta-analysis of rodent studies. The comprehensive search across eight databases ultimately identified 35 eligible studies, coving four different exercises: low-intensity continuous training (LICT), moderate-intensity continuous training (MICT), high-intensity continuous training (HICT), and high-intensity interval training (HIIT). The network meta-analysis indicated that MICT showed superior efficacy versus HICT in terms of sperm concentration (MD: 15.28, 95% CI: 2.52-28.04). The surface under the cumulative ranking curve (SUCRA) value, an indicator to quantify the relative ranking probability of interventions, indicated the highest probability of MICT being optimal training for increasing sperm concentration in both healthy (SUCRA 77.6%) and unhealthy groups (SUCRA 80.1%). Additionally, MICT (MD: 12.23, 95% CI: 7.97-16.48) and HIIT (MD: 10.94, 95% CI: 1.10, 20.78) were superior to sedentary control in improving sperm motility. However, HICT was associated with a significant reduction in normal sperm morphology compared to sedentary control (MD: -10.05, 95% CI: -19.53- -0.58) and MICT (MD: -17.25, 95% CI: -32.17- -2.33). These preclinical results position MICT as the preferred exercise modality for sperm quality improvement, with HIIT as an alternative. However, environmental and lifestyle confounders unique to humans require careful attention when extrapolating these findings to human populations.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Regulatory Function of MicroRNAs in Reproductive System Apoptosis and Their Implications for Infertility.","authors":"Hadis Musavi, Maryam Ezzati, Sheyda Hoseinnia, Mandana Zafari, Zeinab Vahidinia, Melika Izadpanah","doi":"10.1007/s43032-025-01897-5","DOIUrl":"https://doi.org/10.1007/s43032-025-01897-5","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are small non-coding RNA molecules that modulate gene expression and are essential in numerous biological functions, including reproductive functions. Alterations in miRNA expression can affect ovarian function, sperm development, and implantation processes, potentially leading to different diseases and consequently infertility. Researches have indicated that certain microRNAs play a crucial role in the regulation of hormones and proteins that are vital for reproductive processes, suggesting that dysregulation of these miRNAs may contribute to infertility in both men and women. Some miRNAs are able to promote apoptosis, while others may inhibit this process. They can control the expression of genes associated with programmed cell death, thereby influencing the health of oocytes and sperm. Dysfunction of some micro RNAs may lead to increased apoptosis and decreased fertility. The aim of this study was to investigate microRNAs involved in the regulation of apoptosis in male and female reproductive system cells, and to evaluate their association with infertility-related disorders.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracytoplasmic Sperm Injection (ICSI) Timing Affects the Influence of Sperm DNA Fragmentation on ICSI Outcomes.","authors":"Gaofeng Yan, Dan Tang, Zhenfang Su, Longda Wang, Youlin Yao, Wenjuan Yang, Ling Lu, Xiufang Gui, Huawei Wang, Shuhua Zhao","doi":"10.1007/s43032-025-01924-5","DOIUrl":"https://doi.org/10.1007/s43032-025-01924-5","url":null,"abstract":"<p><p>Oocytes have repair ability for paternally derived DNA damage which depends on oocyte quality. Prolonged post ovum pick-up (OPU) incubation may lead to oocyte aging, which is linked to changes in oocyte ultrastructure and function. In order to study whether the effect of sperm DNA fragmentation (SDF) on intracytoplasmic sperm injection (ICSI) outcomes influenced by ICSI timing, we conducted this retrospective study included 1,331 ICSI cycles from March 2016 to December 2022. Outcomes were compared between two groups (an SDF level < 30% group and an SDF level ≥ 30% group) at different tertiles, which were based on OPU-ICSI time. There were no significant between-group differences in the overall laboratory and clinical outcomes (all p > 0.05). However, the clinical pregnancy rates (CPRs) (28.6% vs. 40.9%, p = 0.017) and live birth rates (LBRs) (21.4% vs. 33.4%, p = 0.014) were significantly lower in the SDF level ≥ 30% group than in the SDF level < 30% group in tertile 3. After adjusting for confounders, these differences remained significant, as shown by odds ratios (ORs) (95% confidence intervals (CIs)) of 0.425 (0.244, 0.742) for CPR and 0.485 (0.272, 0.865) for LBR. Significant between-tertile differences in outcomes were only found in the SDF level ≥ 30% group. In conclusion, ICSI timing affects the influence of SDF on the probability of live birth in women undergoing ICSI treatment. Therefore, in patients with high SDF levels, the optimal timing of ICSI should be determined.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Testosterone on Cumulus Cells Gene Expression in Patients with Diminished Ovarian Reserve.","authors":"Bruno Tarasconi, Tatiana C S Bonetti, Dieli Primo, Eduardo L A Motta","doi":"10.1007/s43032-025-01906-7","DOIUrl":"https://doi.org/10.1007/s43032-025-01906-7","url":null,"abstract":"<p><p>Diminished ovarian reserve represents a challenging condition in assisted reproductive technology. Androgens' actions are recognized as instrumental in folliculogenesis based on animal and human studies. Thus, we aimed at investigating whether testosterone pretreatment has an influence on gene expression of cumulus cells of diminished ovarian reserve patients. We included patients under 42 years-old presenting for IVF with diminished ovarian reserve (antral follicle count ≤ 6 and antimüllerian hormone < 1.2 ng/ml), between may/2018 and august/2019. Stratified randomization was performed and subjects were allocated into testosterone group (receiving transdermal testosterone gel pretreatment 1% 25 mg daily; N = 9) or control group (receiving standard ovarian stimulation; N = 9). Cumulus cells were collected following oocyte retrieval and RT-qPCR was conducted to evaluate gene expression differences in members of AKT signaling pathway, mTOR signaling pathway, IGF1, FSHR and AMH. Testosterone group patients showed a lower expression of AKT1 in cumulus cells as compared to control group subjects. Other evaluated target genes of the same pathway (FOXO1, FOXO3, PTEN), mTOR signaling pathway genes (mTOR, TSC1, TSC2), as well as IGF1, FHSR and AMH displayed no significant differences between groups. Testosterone pretreatment in diminished ovarian reserve patients down-regulates AKT1 gene expression in cumulus cells. The described profile could be linked to better oocyte reproductive potential, as cumulus cells related to competent oocytes might decide their fate (apoptosis) after completing their role in sustaining oocyte development during cyclic follicular recruitment. Transdermal testosterone pretreatment in diminished ovarian reserve patients down-regulates AKT1 in cumulus cells, possibly enhancing oocyte reproductive potential.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of hub genes and potential molecular mechanisms of tumor immune microenvironment-related radiotherapy sensitivity in locally advanced cervical cancer.","authors":"Yichen Zan, Qing Liu, Yue Yin, Chongxin Guo, Jing Xu, Junhan Gao, Guanglu Dong","doi":"10.1007/s43032-025-01909-4","DOIUrl":"https://doi.org/10.1007/s43032-025-01909-4","url":null,"abstract":"<p><p>Cervical cancer remains the most prevalent malignancy of the female reproductive system; however, the five-year survival rate has not improved substantially over recent decades. This persistent challenge highlights the critical need to elucidate mechanisms underlying treatment sensitivity and investigate synergistic effects of multimodal therapies to overcome clinical therapeutic bottlenecks. By conducting a comprehensive analysis of The Cancer Genome Atlas (TCGA) database, this study identified a hub gene network associated with radiotherapy sensitivity in cervical cancer. In addition to developing a personalized treatment prediction model, we preliminarily characterized these hub genes through several key dimensions: molecular regulatory, biological functions, tumor microenvironment infiltration patterns, and immune relevance. Notably, this work is the first to reveal that hub genes such as DDX58 probably orchestrate a tripartite regulatory mechanism-mediated by immune microenvironment factors-that concurrently influences sensitivity to radiotherapy, chemotherapy, and immunotherapy. Through a series of analyses, we speculate that these hub genes can serve not only as combined biomarkers for predicting responses to multiple therapies but also as novel molecular targets for the development of synergistic treatment strategies via their mediated immune microenvironment interactions. These findings provide a new translational research direction for overcoming therapeutic resistance in cervical cancer.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homeobox C6 is Up-Regulated and Affects the Pathogenesis of Endometriosis.","authors":"Jianfa Jiang, Luyao Zhang, Lijie Li","doi":"10.1007/s43032-025-01918-3","DOIUrl":"https://doi.org/10.1007/s43032-025-01918-3","url":null,"abstract":"<p><p>Endometriosis is one of the most common, difficult, and complicated gynecological disorders. The present study investigated the expression of HOXC6 in endometriosis and described its possible role in its pathogenesis. Ectopic and eutopic endometrial samples from 21 patients with endometriosis and a control endometrium from 15 women without endometriosis were collected. HOXC6 expression in endometrial tissue was analyzed with immunohistochemistry and quantitative reverse transcription polymerase chain reaction. HOXC6's silencing in endometriotic stromal cells (ESC) was established with small interfering RNA (siRNA) to detect the effect on proliferation, adhension, invasion, and migration using CCK-8, adhesion, wound healing, and transwell assays. Western blotting was performed to detect the expression of related molecules after the down-regulation of HOXC6. HOXC6 mRNA and protein expression levels in the ectopic endometrial samples were significantly higher in women with endometriosis than in controls, while the levels in the eutopic endometrial tissues of the same patients did not significantly differ from those in non-endometriotic patients. The knockdown of HOXC6 expression inhibited the proliferation, adhesion, migration, and invasion of ESC. The results indicated that HOXC6 down-expression decreased the expression of N-cadherin and vimentin, whereas the expression of E-cadherin increased. HOXC6 down-expression also decreased the expression levels of TGF-β1 and phosphorylated SMAD2/SMAD3. In conclusion, HOXC6 was overexpressed in endometriosis and might therefore promote the proliferation, adhesion, invasion, and migration of ESC via the TGF-β1/smad signaling pathway. These findings present a new perspective and may therefore inspire further study of the endometriosis mechanism.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Profiling Reveals Potential Genes Involved in the Immune Landscape of Polycystic Ovary Syndrome: An Exploratory Study.","authors":"Ye Zhang, Zhiyang Hu, Zebo Cai, Junda Lai, Huiqiong Zeng","doi":"10.1007/s43032-025-01917-4","DOIUrl":"https://doi.org/10.1007/s43032-025-01917-4","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"m5C-Modified lncRNA SNHG15 Promotes Ovarian Cancer Progression Via the miR-545-3p/PD-L1 Axis.","authors":"Li Chen, Ruifeng Ma, Lei Wu, Dandan Wang, Jingchao Li, Li Guo","doi":"10.1007/s43032-025-01919-2","DOIUrl":"https://doi.org/10.1007/s43032-025-01919-2","url":null,"abstract":"<p><p>Ovarian cancer (OC) poses a health burden of women. Long non-coding RNA SNHG15 has been reported to promote OC progression; however, its effect on immune evasion remains unknown. Here, we investigated the effect of SNHG15 on OC cell immune evasion and the underlying mechanism. Cell phenotypes were assessed using cell counting kit-8, colony formation, lactate dehydrogenase cytotoxicity, and enzyme-linked immunosorbent assays. The mechanism was evaluated by dual-luciferase reporter assay, RNA pull-down, RNA immunoprecipitation (RIP), methylated RIP, and RNA stability assay. The role in vivo was analyzed using tumor-bearing mice. The results showed that SNHG15 and PD-L1 levels were increased, while miR-545-3p expression was reduced in OC. SNHG15 was a sponge of miR-545-3p, and PD-L1 was a downstream target of SNHG15. Knockdown of SNHG15 suppressed OC cell proliferation, and enhanced cytotoxicity and pro-inflammatory response of CD8⁺ T cells, whereas miR-545-3p downregulation reversed these cellular behaviors. Moreover, PD-L1 reversed the cell phenotypes induced by miR-545-3p. Additionally, SNHG15 was modified by m5C, which was mediated by NSUN2. Furthermore, SNHG15 knockdown impeded tumor growth in mice. In conclusion, m5C-methylated lncRNA SNHG15 promotes OC progression by accelerating cell proliferation and immune evasion via the miR-545-3p/PD-L1 axis, demonstrating a tumor-promoting function of SNHG15 in OC.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial Effects of Alchemilla vulgaris in DHEA-Induced Rat Model of Polycystic Ovary Syndrome.","authors":"Zeynep Ece Utkan Korun, Semil Selcen Gocmez, Selenay Furat, Kubra Kavram Sarihan, Fatma Ceyla Eraldemir, Huseyin Askin Akpulat, Deniz Sahin, Sule Yildiz","doi":"10.1007/s43032-025-01885-9","DOIUrl":"https://doi.org/10.1007/s43032-025-01885-9","url":null,"abstract":"<p><p>Alchemilla vulgaris has traditionally been used to treat gynecologic diseases. The anti-inflammatory and vasorelaxant effects of the extract of A. vulgaris (AVE) have been reported in previous studies. This study aimed to investigate the effects of A. vulgaris on PCOS, one of the most common metabolic disorders in adult women of reproductive age, accompanied by inflammation and vascular endothelial dysfunction, in an experimental animal model. Three groups of prepubertal female Wistar rats were established: PCOS, Control, and PCOS + AVE groups. Subcutaneous dehydroepiandrosterone (DHEA) was administered for 35 days to the PCOS and PCOS + AVE groups. The rats in the PCOS + AVE group were orally administered AVE (50 mg/100 g bw/day) for 35 days. Systemic blood pressure, vascular endothelial function, ovarian morphology, and endocrine biomarker levels were measured at the end of the experiment. TNF-α levels were also evaluated in the thoracic aorta and ovaries. Hormonal imbalances, ovarian morphological changes, and endothelial dysfunction were observed in the PCOS group, which returned to the controls after AVE treatment. Furthermore, AVE attenuated the aortic and ovarian levels of TNF-α in the PCOS group. Our study suggests that AVE may have beneficial effects in the treatment of PCOS due to its anti-inflammatory and vasculoprotective properties.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Ambient Ozone Pollution Exposure on Mice Testicular Tissue: An Integrated Metabolomic and Transcriptomic Analysis.","authors":"Xiaoqin Feng, Jinhong Luo","doi":"10.1007/s43032-025-01907-6","DOIUrl":"https://doi.org/10.1007/s43032-025-01907-6","url":null,"abstract":"<p><p>The real ozone concentration in the air is rising yearly globally, which remarkably has adverse effect on human health, particularly on male fertility. Epidemiological studies showed that men exposure to ambient ozone was linked with decreased semen quality. Therefore, it is urgent to elucidate the toxicological evaluation of ozone exposure on men reproduction. This study conducted transcriptomic mRNA sequencing and high-performance liquid chromatography-tandem mass spectrometry to depict the characteristics of metabolomics and transcriptomics of mice testis tissue exposed to ozone. In transcriptomic, KEGG showed that spermatogenesis was significantly influenced by ozone exposure, meanwhile dysregulation of protein serine/threonine kinase activity, intracellular signal transduction, cell projection organization, and regulation of microtubule polymerization were observed, these molecular events participate in regulation and control of spermatogenesis. In metabolomic, twenty differential metabolites were the top abundance ranking features, as intermediate products of phospholipid metabolism to act as signal molecules, participating in intracellular signal transduction pathways, may affect the fluidity and structure of sperm cell membrane. Integrated omics showed that aminoacyl-tRNA biosynthesis was the main pathway attributed to significant correlation between transcriptomics and metabolomics (combination p value = 0.01), including arginine biosynthesis, essential for spermatogenesis. In integrated omics, several signatures were identified to be associated with spermatogenesis, including Creb3l4, Pgp and Tcp1, contributing to maturation of sperm head nuclei, energy metabolism and formation of sperm flagellum. The finding suggested ambient ozone exposure significantly altered the characteristics of transcriptome and metabolome of mice testis, and impaired spermatogenesis. Our finding provided new evidence of more stringent control and management for ambient ozone so as to reduce the potential risk of declined male fertility.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}