Reproductive Sciences最新文献

{"title":"Artificial Uterus and Artificial Embryos: Unsolved Tasks.","authors":"Maxim A Filatov, Iuliia P Baikova, Daria M Dolmatova","doi":"10.1007/s43032-025-01939-y","DOIUrl":"https://doi.org/10.1007/s43032-025-01939-y","url":null,"abstract":"<p><p>Ex utero embryo culture is one of the important tasks that has not been completely solved. In the first part of the current review, we focus on potential roles ex utero mammalian embryo culture could play, such as in infertility treatment or ex utero embryo treatment. Particularly, we discuss ex utero genetic therapy of embryos for correction and prevention of congenital pathologies. We also discuss the benefits of ex utero embryo culture in comparison to the traditional surrogacy programs and review its potential influence on social aspects. In the second part, we discuss key methods for ex utero embryo culture, their limitations, bottlenecks and blank points. Moreover, we analyze methods of artificial embryo production using stem cells and possible uses of such embryos (including those that reproduce stages of postimplantation development) in scientific research. We discuss the risks of using the described technologies and possible approaches that will avoid these risks.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment Outcomes and Resistance Patterns in Low Risk GTN: A 270-Patient Experience from a Tertiary Center.","authors":"Elham Shirali, Fariba Yarandi, Sara Ramhormozian, Atena Abedini","doi":"10.1007/s43032-025-01960-1","DOIUrl":"https://doi.org/10.1007/s43032-025-01960-1","url":null,"abstract":"<p><p>Because of the lack of fully successful treatment of low-risk gestational trophoblastic neoplasia (GTN) patients after first-line single-agent chemotherapy, some studies have suggested that these patients should be treated with multi-drug chemotherapy from the outset. A score of 0-4 represents the majority of low risk GTN that has been less studied. The present study aimed to investigate the risk factors associated with resistance to first-line single-agent chemotherapy. This retrospective cohort includes patients diagnosed with low-risk GTN who were treated with single-agent chemotherapy using methotrexate and actinomycin. Factors associated with resistance to first-line chemotherapy were analyzed separately in two groups: those with a score of 0-6 and those with a score of 0-4. A total of 270 patients were studied, all of whom achieved remission. Of these, 75.9% responded to first-line single-agent chemotherapy. Patients aged 40 or older had about 7 times higher odds of treatment resistance than younger patients. Choriocarcinoma increased the odds by 5.5 times, and each 1 cm increase in tumor size raised the odds by 53%. In patients with WHO scores of 0-4, each additional year of age increased resistance odds by 6%, and choriocarcinoma raised it by 7.5 times. In both groups, lung metastasis increased the chance of resistance threefold. Increased age, larger tumor size, increase βhCG level and start of treatment with MTX, increase the likelihood of resistance to first-line chemotherapy in the 0-6 score and in the subgroup with a 0-4 score. Future prospective studies are necessary to validate new models Until then, clinicians should be aware of the limitations of the current system and consider individualized clinical judgment.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Role of TLR4 Asp299Gly and Thr399Ile Gene Polymorphisms as a Risk Factor in Human Cytomegalovirus Positive Pregnant Women.","authors":"Tanzeem Fatima, Asad Syed, Abdallah M Elgorban, Islem Abid, Ling Shing Wong, Mohd Sajid Khan, Jahanarah Khatoon, Punit Kumar Singh","doi":"10.1007/s43032-025-01963-y","DOIUrl":"https://doi.org/10.1007/s43032-025-01963-y","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Study of MiR-30c-5p Regulation of SIRT Expression in Polycystic Ovary Syndrome.","authors":"Lifei Zhou, Bo Zheng, Yan Luo, Pingping Zhang, Fangfang Dai, Mingming Zhang, Shusong Wang, Yali Li","doi":"10.1007/s43032-025-01932-5","DOIUrl":"https://doi.org/10.1007/s43032-025-01932-5","url":null,"abstract":"<p><p>Abnormal development of granulosa cells is widely recognized as a critical factor contributing to polycystic ovary syndrome (PCOS). However, the precise etiology and underlying mechanisms of this disorder remain largely elusive. Accumulating evidence suggests that dysregulation of microRNAs (miRNAs) plays a pivotal role in the pathogenesis of PCOS. In this study, we systematically investigated the functional impact of miR-30c-5p on the human cumulus cells (CCs). Our findings revealed that miR-30c-5p suppresses the proliferation and induces apoptosis in the human granulosa-like tumor cell line (KGN) via targeting SIRT1. Notably, the expression level of miR-30c-5p was significantly elevated in PCOS patients compared to healthy controls, whereas the expression of SIRT1 was markedly reduced. A negative correlation was observed between miR-30c-5p and SIRT1 expression. Mechanistically, upregulation of miR-30c-5p led to decreased expression of SIRT1 and Bcl-2 proteins, while simultaneously enhancing the expression of Bax proteins. Furthermore, our data confirmed that SIRT1 serves as a direct target of miR-30c-5p. Collectively, these results indicate that miR-30c-5p promotes apoptosis of GCs by directly targeting SIRT1, thereby representing a novel molecular target for improving GC dysfunction in PCOS patients.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventing Preterm Birth: The Search for Tocolytic Synergism.","authors":"Md Reduanul Hossain, Marina Paul, Jorge M Tolosa, Roger Smith, Jonathan W Paul","doi":"10.1007/s43032-025-01941-4","DOIUrl":"https://doi.org/10.1007/s43032-025-01941-4","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Piceatannol Protects Sperm from Cryopreservation Damage by Modulating the Keap1-Nrf2/ARE Signaling Pathway.","authors":"Lijie Fu, Chao Wang, Wenfu Li, Hao Dong, Qian Yang, Guilin Chang, Jianping Liu","doi":"10.1007/s43032-025-01961-0","DOIUrl":"https://doi.org/10.1007/s43032-025-01961-0","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Second Clinical Pregnancy Following Repeat Ovarian Tissue Transplantation in an Acute Leukemia Survivor: First Report and Literature Overview.","authors":"Meltem Sönmezer, Batuhan Aslan, Nilüfer Akgün, Yavuz Emre Şükür, Özgür Çınar, Ebru İbiş, Cem Somer Atabekoğlu, Murat Sönmezer","doi":"10.1007/s43032-025-01962-z","DOIUrl":"https://doi.org/10.1007/s43032-025-01962-z","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Clinical Characteristics and Molecular Aetiology of Cytochrome P450 Oxidoreductase Deficiency Diagnosed in 46,XX Patients.","authors":"Duoduo Zhang, Leilei Ding, Shan Deng, Qinjie Tian","doi":"10.1007/s43032-025-01950-3","DOIUrl":"https://doi.org/10.1007/s43032-025-01950-3","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadotropin Releasing Hormone-Antagonist Protocol (GnRH-ant) Combined Letrozole (LE) for Expected High Ovarian Responders (HOR): a Cohort Study.","authors":"Wenxiao Feng, Min Zhou, Qiuying Li, Hui Chen, Jinyu Lu, Yang Sui","doi":"10.1007/s43032-025-01922-7","DOIUrl":"10.1007/s43032-025-01922-7","url":null,"abstract":"<p><p>High ovarian responders are at high risk of developing ovarian hyperstimulation syndrome (OHSS) in in vitro fertilization (IVF). Letrozole administration during the gonadotropin period has the theoretical basis to avoid OHSS. But researches are just beginning. Our prospective cohort study was conducted to explore if the addition of LE are better or not. Totally 107 high responders who received IVF were included, 66 patients for GnRH-ant protocol and 41 patients for co-LE protocol with letrozole combination during gonadotropin period from day 1 to day 5. The IVF outcomes appeared: the clinical pregnancy rate, cumulative pregnancy rate, live birth and ongoing pregnancy rate were both comparable (p > 0.05). Patients in the co-LE group exhibited significantly less gonadotropin dose (1464.39 ± 550.84 vs. 1708.23 ± 455.75, p = 0.015), gonadotropin duration (9.63 ± 2.11 vs. 10.48 ± 1.83 days, p = 0.032) and oocytes number (13.29 ± 10.30 vs. 18.18 ± 9.36, p = 0.013). The incidence of early OHSS was significantly lower than that in the GnRH-ant group (0% & 13.6%, p = 0.025). According to our research, the combination of letrozole can improve controlled ovarian stimulation (COS) efficiency and reduce the risk of OHSS while bring effective IVF clinical outcomes equivalent to GnRH-ant regimen for ovarian hyperresponders.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2793-2801"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Improvement of Menstrual Blood Derived Endometrial Stem Cells Encapsulated in Cross-Linked Hyaluronic Acid on Intrauterine Adhesions.","authors":"Ying Pan, Mengyuan Chang, Wenqiang Zhang, Shenghui Zhang, Pin Chang, Hongbin Cheng, Xiao Gao, Wenqiang Fan, Wenxiang Wang, Yanli Liu, Juntang Lin","doi":"10.1007/s43032-025-01928-1","DOIUrl":"10.1007/s43032-025-01928-1","url":null,"abstract":"<p><p>Intrauterine adhesions (IUA), characterized by fibrotic adhesions within the uterine cavity, frequently result in menstrual irregularities, recurrent miscarriages, secondary infertility, and increased pregnancy risks among women of childbearing age. Unfortunately, conventional clinical interventions mainly including surgical separation and physical isolation exhibit limited efficacy in promoting the regeneration of the injured endometrium and are associated with a high recurrence rate. Consequently, it is imperative to investigate novel therapies aimed at repairing the injured endometrium in IUA patients, particularly those desiring fertility. Recently, stem cell-based therapies have exhibited promise due to their superior paracrine action and immunomodulation, emerging as a new alternative for tissue regeneration and anti-fibrosis treatment. Herein, we first confirmed the excellent biocompatibility of menstrual blood-derived endometrial stem cells (MenSCs) when combined with XLHA-a hyaluronic acid-based scaffold. The 3D microenvironment provided by XLHA enhanced the immunomodulation of MenSCs and prolonged their retention in uterine cavity of IUA mice. Subsequently, MenSCs encapsulated in XLHA exhibited significantly improved therapeutic outcomes compared to MenSCs or XLHA alone. This approach not only facilitated recovery of endometrial morphology and increased the number of endometrial glands but also ameliorated the apoptosis and inflammation in the endometrium of IUA mice. Furthermore, the transplantation of MenSCs encapsulated in XLHA significantly improved fertility outcomes, likely due to the improved endometrial receptivity in IUA mice. In conclusion, this study confirms that the transplantation of MenSCs encapsulated in XLHA effectively ameliorates endometrial injury in IUA mice while elucidating the potential mechanisms involved. This research provides a practical and cost-effective delivery strategy for enhancing the therapeutic efficiency of MenSCs in IUA treatment.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2703-2716"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}