Reproductive Sciences最新文献_第7页

Beneficial Effects of Pomegranate Extracts for Benign Gynecologic Disorders. 石榴提取物对良性妇科疾病的有益作用。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2024-12-28 DOI: 10.1007/s43032-024-01776-5

Samya El Sayed, Valeria I Macri, Bhuchitra Singh, James H Segars, Md Soriful Islam

{"title":"Beneficial Effects of Pomegranate Extracts for Benign Gynecologic Disorders.","authors":"Samya El Sayed, Valeria I Macri, Bhuchitra Singh, James H Segars, Md Soriful Islam","doi":"10.1007/s43032-024-01776-5","DOIUrl":"10.1007/s43032-024-01776-5","url":null,"abstract":"Pomegranate (Punica granatum) is a widely cultivated fruit historically recognized for its health benefits and is regarded as a nutritional powerhouse. Pomegranate has a unique composition of bioactive compounds including hydrolysable tannins, anthocyanins, and other polyphenolic components. Of those, punicalagin and its subsequent metabolites are the most extensively studied, demonstrating antioxidant, anti-inflammatory, anti-cancer, and anti-nociceptive activity. The compounds possess promising therapeutic potential for many diseases, including conditions affecting the female reproductive system. This scoping review examines the pharmacodynamics of pomegranate's bioactive compounds and synthesizes the current literature concerning the role in benign gynecological disorders. Pomegranate extract decreased testosterone levels, levels of oxidative stress and inflammation biomarkers in women with polycystic ovary syndrome, erstwhile favorably impacting some cardiovascular risk factors in women. Pomegranate supplementation improved menopause specific health-related quality of life in women. In a pre-clinical murine model following ovariectomy, improved bone formation and reduced vaginal atrophy were associated with pomegranate treatment. Existing data suggests that additional research on the beneficial antioxidant, anti-inflammatory, anti-proliferative, and anti-nociceptive effects of pomegranate extracts for benign gynecologic conditions is warranted.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"600-617"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Kisspeptin and KISS₁ Gene Polymorphism (rs35431622) with Circulating Sex Hormones and Male Infertility.

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI: 10.1007/s43032-025-01803-z

N Prasath, K A Arul Senghor, V M Vinodhini, M Anuradha

{"title":"Association of Kisspeptin and KISS1 Gene Polymorphism (rs35431622) with Circulating Sex Hormones and Male Infertility.","authors":"N Prasath, K A Arul Senghor, V M Vinodhini, M Anuradha","doi":"10.1007/s43032-025-01803-z","DOIUrl":"10.1007/s43032-025-01803-z","url":null,"abstract":"This research delves into the association of Kisspeptin and the KISS1 gene (rs35431622) single nucleotide polymorphism with circulating sex hormones and semen parameters in males diagnosed with infertility. Eighty male participants were recruited from fertility clinic, were divided into two groups: Group A, characterized by normal sperm count and Group B, exhibiting low count. The analysis involved assessing hormone levels Kisspeptin, follicle stimulating hormone, luteinizing hormone, total testosterone and sex hormone binding globulin by utilizing ELISA, investigating the association of the KISS1 gene SNP with these variables through Tetra amplification refractory mutation system-PCR and Gel Electrophoresis. The findings revealed that males a low sperm count displayed notably reduced levels of Kisspeptin and free testosterone, accompanied by increased luteinizing hormone and follicle stimulating hormone. The KISS1 gene SNP rs35431622 (Q36R) demonstrated a 53.3% frequency of the heterozygous mutant TA genotype and OR 0.19 (CI 0.05-0.71) among infertile males, indicating its potential implications a risk factor for male infertility. This study emphasizes the significance of Kisspeptin in overseeing male reproductive health and highlights its potential as a marker for hormonal dysregulation in male infertility.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"748-756"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Endometrial Polyp and Myoma Uteri on Fertility-Related Genes in the Endometrium.

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-06 DOI: 10.1007/s43032-025-01802-0

Gürkan Özbey, Görkem Tuncay, Senem Arda Düz, Yılmaz Çiğremiş, Abdullah Karaer

{"title":"The Effect of Endometrial Polyp and Myoma Uteri on Fertility-Related Genes in the Endometrium.","authors":"Gürkan Özbey, Görkem Tuncay, Senem Arda Düz, Yılmaz Çiğremiş, Abdullah Karaer","doi":"10.1007/s43032-025-01802-0","DOIUrl":"10.1007/s43032-025-01802-0","url":null,"abstract":"Endometrial polyps are hyperplastic overgrowths of the endometrium that contain both glands and stroma. Myoma uteri is the most common benign tumor of the female pelvis and uterus. HOXA10, which is involved during the organogenesis of the uterus in the embryonic period. The aim of this study was to compare the expression levels of infertility-related genes in endometrial tissue obtained from patients with endometrial polyp and myoma uteri and from healthy controls. A total of 36 patients, including 15 women with endometrial polyp and 21 women with myoma uteri, and 23 healthy controls were enrolled in the study. All patients were evaluated using transvaginal ultrasonography. Endometrial tissue samples were collected from the patient and control groups between the 19th and 21st days of the menstrual cycle. Expression levels of the receptivity markers PROK1, PROKR1, PROK2, PROKR2 and HOXA10 genes were determined by RT- PCR. When the patients diagnosed with endometrial polyp and the healthy controls were compared, it was observed statistically significantly that the expression of PROKR1 increased in endometrium tissue of patients with endometrial polyp (p < 0.05). In patients diagnosed with myoma uteri, gene expression levels of endometrial PROKR1 was statistically significant increased and gene expression levels of PROK1, PROKR2, HOXA10 were found to be statistically significantly decreased compared to the controls (p < 0.05). Changes in the endometrial expression of the HOXA10 and prokineticin gene family in patients with myoma uteri and endometrial polyps may explain certain aspects of infertility in these patients.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"728-737"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Renaissance of Reproductive Science: Leonardo da Vinci's Anatomical Contributions. 生殖科学的复兴：列奥纳多·达·芬奇的解剖学贡献。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-16 DOI: 10.1007/s43032-024-01772-9

Michael Carroll

{"title":"The Renaissance of Reproductive Science: Leonardo da Vinci's Anatomical Contributions.","authors":"Michael Carroll","doi":"10.1007/s43032-024-01772-9","DOIUrl":"10.1007/s43032-024-01772-9","url":null,"abstract":"Leonardo de Vinci is considered one of the greatest artists that has ever lived. His work includes the Mona Lisa and The Last Supper are celebrated worldwide. His talents expand beyond his art and his contributions to disciplines such as engineering, astronomy, geometry, botany, geology have revealed is him as a polymath. One discipline where he devoted a considerable amount of time and effort was anatomy. His anatomical notes and drawings can be paralleled to texts books of today. This review explores Leonardo da Vinci's significant contributions to the study of human anatomy, with a particular focus on reproductive anatomy. Leonardo's meticulous dissections and detailed anatomical sketches, despite being influenced by the limited scientific understanding and constraints of his time, reveal his deep curiosity and innovative approach. His work, though not widely recognised during his lifetime, laid foundational insights into neuroanatomy, the cardiovascular system, and foetal development. He had a particular interest in reproduction and the genitourinary anatomy and made many anatomical studies in this area. The review also examines the challenges Leonardo faced, such as the lack of preservation techniques and the influence of Galenic teachings, which led to both groundbreaking observations and notable inaccuracies in his anatomical studies or the reproductive system.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"575-599"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

25-hydroxyvitamin D and Endometriosis: A bidirectional Mendelian randomization study. 25- 羟维生素 D 与子宫内膜异位症：双向孟德尔随机研究

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2024-03-21 DOI: 10.1007/s43032-024-01517-8

Di Pan, Pan Li, Xiaoling Dai, Shiyun Xie

{"title":"25-hydroxyvitamin D and Endometriosis: A bidirectional Mendelian randomization study.","authors":"Di Pan, Pan Li, Xiaoling Dai, Shiyun Xie","doi":"10.1007/s43032-024-01517-8","DOIUrl":"10.1007/s43032-024-01517-8","url":null,"abstract":"Numerous studies have demonstrated a correlation between serum 25-hydroxyvitamin D (25OHD) and endometriosis. However, the precise nature of this association remains elusive. The causal connection between 25OHD and endometriosis remains uncertain, as it is yet to be determined whether one directly influences the other. The objective of our research was to investigate the cause-and-effect connection between 25OHD and endometriosis. The study employed Mendelian randomization (MR) in a bidirectional two-sample investigation to examine the causal relationship between 25OHD and endometriosis. The analysis utilized the most recent publicly accessible statistics from the genome-wide association study (GWAS) encompassing 25OHD, endometriosis, and its five subtypes. The primary analytical approach employed was Inverse-Variance Weighting (IVW), accompanied by supplementary analysis methods including weighted median, MR-Egger, simple mode, and weighted mode. Furthermore, sensitivity analyses were conducted to assess the potential influence of heterogeneity and pleiotropy on the MR outcomes. MR primary analysis showed no significant causal effect of 25OHD on endometriosis (OR = 0.892, 95%CI = 0.745 ~ 1.068, P = 0.213). Similarly, there was no evidence to support a causal relationship of endometriosis on 25OHD (IVW Beta = 0.005, 95%CI = 0.993 ~ 1.018, P = 0.406). However, when conducting MR analysis on different subtypes of endometriosis and 25OHD, we found a positive correlation between endometriosis of ovary and 25OHD level (IVW Beta = 0.012, 95%CI = 1.002 ~ 1.022, P = 0.024). This study indicates that there is no causal relationship between serum 25OHD and endometriosis. However, it is important to note that serum 25OHD levels will increase in patients with endometriosis of the ovary. Further observational studies and clinical trials are indispensable.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"693-701"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MT1/cAMP/PKA Pathway in Melatonin-Regulated Sperm Capacitation. 褪黑激素调控精子获能的MT1/cAMP/PKA通路

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-21 DOI: 10.1007/s43032-024-01782-7

Tongjuan Niu, Di Zhang, Guobin Qiu, Bin Li, Sheng Cui

{"title":"MT1/cAMP/PKA Pathway in Melatonin-Regulated Sperm Capacitation.","authors":"Tongjuan Niu, Di Zhang, Guobin Qiu, Bin Li, Sheng Cui","doi":"10.1007/s43032-024-01782-7","DOIUrl":"10.1007/s43032-024-01782-7","url":null,"abstract":"Melatonin is mainly synthesized and secreted by pineal gland, and plays multiple functions, including its regulating effects on reproductive processes. Sperm capacitation is necessary for fertilization, but the effect of melatonin on mouse sperm capacitation remains to be elucidated. We thus investigated the roles of melatonin on capacitation by culturing the sperms from mouse cauda epididymis in the medium with different doses of melatonin. The results showed that 10-7 mol/L melatonin significantly enhanced the sperm capacitation by increasing the sperm tyrosine phosphorylation level, percentage of the capacitated sperms and intracellular calcium concentration. In addition, our in vitro and in vivo results showed that melatonin enhanced the fertilizing capacity by increasing the percentage of oocyte cleavage and the number of the fetuses from receptive females which were mated with melatonin-treated males. Mechanically, melatonin activated cyclic adenosine monophosphate (cAMP)/p-Protein kinase A (p-PKA) pathway. Furthermore, the melatonin-induced tyrosine phosphorylation of sperms was decreased by treatment of MT1 or PKA inhibitor. And the same result was found in the sperms cultured in non-capacitating medium (without bicarbonate and bovine serum albumin). Therefore, all those results indicate that MT1/cAMP/PKA pathway is one of key regulatory factors in melatonin-mediated sperm capacitation. The results here are crucial for understanding the molecular mechanisms by which melatonin regulates sperm capacitation, and providing theoretical support for controlling sperm capacitation during artificial insemination procedures.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"792-803"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEIS1-mediated Apoptosis via TNFR1 in Endometriosis.

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-11 DOI: 10.1007/s43032-025-01801-1

Wenwen Wang, Fangfang Fu, Yan Li, Sha Li, Ming Yuan, Tian Wang, Wu Ren, Jia Wei, Dan Chen, Shixuan Wang, Xiangyi Ma, Zhangying Wu

{"title":"MEIS1-mediated Apoptosis via TNFR1 in Endometriosis.","authors":"Wenwen Wang, Fangfang Fu, Yan Li, Sha Li, Ming Yuan, Tian Wang, Wu Ren, Jia Wei, Dan Chen, Shixuan Wang, Xiangyi Ma, Zhangying Wu","doi":"10.1007/s43032-025-01801-1","DOIUrl":"10.1007/s43032-025-01801-1","url":null,"abstract":"Abnormal apoptosis both maintains endometrial cell growth and induces endometrial pathogenesis. The etiology of endometriosis is unclear and no treatment is curative. Therefore, the aim herein was to identify genes involved in the pathogenesis of endometriosis. Using the data from our previous results and RNA sequencing data of normal endometrial tissue and ovarian endometrioma (OMA) tissue, along with Gene Expression Omnibus (GEO) dataset on endometriosis, we identified an apoptotic-related gene, meis homeobox I (MEIS1). Normal endometrium, eutopic endometrium and ectopic endometriotic tissues were used to detect MEIS1. Primary normal endometrial and eutopic endometrial stromal cells were isolated and cultured for exploring the function of MEIS1 and related pathways. A mouse endometriosis model was used to verify the therapeutic effects of MEIS1. The mRNA and protein of MEIS1 in tissues from patients with endometriosis were decreased. Overexpression of MEIS1 induced the apoptosis of primary eutopic endometrium stromal cells by regulating TNFR1. Using Cell Counting Kit 8 (CCK8) assay and EdU assay, we found that knockdown of MEIS1 promoted the proliferation of primary normal endometrium stromal cells. We also observe that upregulated MEIS1 may lead to caspase pathway activation, promoting endometrial cell apoptosis. Furthermore, MEIS1 lentivirus inhibited endometriotic lesion formation and induced apoptosis in the mouse endometriosis model. These cumulative findings suggest that MEIS1 may mediate apoptosis by initiating TNFR1 in endometrial cells via the caspase pathway.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"716-727"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal Stromal Cell Exosome-Induced Vascular Regeneration in a PCOS Mouse Model. 间充质基质细胞外泌体诱导多囊卵巢综合征小鼠模型的血管再生。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2024-10-15 DOI: 10.1007/s43032-024-01720-7

Xiaojing Teng, Xiaolei Wang, Zhiyi Wang

{"title":"Mesenchymal Stromal Cell Exosome-Induced Vascular Regeneration in a PCOS Mouse Model.","authors":"Xiaojing Teng, Xiaolei Wang, Zhiyi Wang","doi":"10.1007/s43032-024-01720-7","DOIUrl":"10.1007/s43032-024-01720-7","url":null,"abstract":"The efficacy of Bone Marrow Mesenchymal Stem Cell-derived Exosomes (BMSCs-Exo) in addressing the complexities of Polycystic Ovary Syndrome (PCOS) has been explored in a controlled experimental study using a DHEA-induced PCOS model in 6-8-week-old female NMRI mice. This research undertook an in vivo approach with fifteen female murine subjects to investigate the potential of BMSCs-Exo in promoting vascular regeneration and alleviating the adverse effects associated with PCOS. Through a strategic intervention, the study aimed to modulate the pathophysiological markers of oxidative stress and inflammation that are hallmark features of PCOS. Remarkably, the administration of BMSCs-Exo led to decreased CD31 expression in ovarian tissues, suggesting reduced angiogenesis and endothelial activation. Moreover, a significant reduction in pro-inflammatory cytokines and oxidative stress markers was noted, aligning closely with the metrics observed in the control group. These findings illuminate a promising therapeutic avenue utilizing BMSCs-Exo to recalibrate angiogenic, inflammatory, and oxidative stress responses in PCOS. This research not only contributes to the current understanding of PCOS management but also opens new doors for innovative clinical treatments.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"825-835"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing the Mechanisms Involved in the Environmental Exposure to Polychlorinated Biphenyls (PCBs) in the Risk of Male Infertility.

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI: 10.1007/s43032-025-01794-x

Enayat Anvari, Zahra Noorimotlagh, Seyyed Abbas Mirzaee, Heshmatllah Nourmoradi, Mona Bahmani, Nasrin Rashan, Susana Silva Martinez, Shiva Kamran, Iraj Ahmadi

{"title":"Establishing the Mechanisms Involved in the Environmental Exposure to Polychlorinated Biphenyls (PCBs) in the Risk of Male Infertility.","authors":"Enayat Anvari, Zahra Noorimotlagh, Seyyed Abbas Mirzaee, Heshmatllah Nourmoradi, Mona Bahmani, Nasrin Rashan, Susana Silva Martinez, Shiva Kamran, Iraj Ahmadi","doi":"10.1007/s43032-025-01794-x","DOIUrl":"10.1007/s43032-025-01794-x","url":null,"abstract":"Exposure to toxic chemicals, such as plasticizers, alkylphenol compounds, and polychlorinated biphenyls (PCBs), has increased due to environmental contamination. PCBs, categorized as persistent organic pollutants (POPs), are lipophilic chemicals commonly used in lubricants, cutting oils, and electrical insulators. PCBs may have detrimental effects on hormone-producing glands, potentially contributing to male infertility. Thus, the objective of this study was to provide a comprehensive overview of the adverse effects of PCBs on the male reproductive system. Searches of three electronic databases were performed using MESH terms and 32 studies were included. Although the exact mechanism of action for PCBs remains unclear, several PCBs are regarded as potential endocrine disruptors due to their ability to interact with hormone signaling pathways. PCBs have been found to disrupt physiological functions by mimicking endogenous hormones as agonists or antagonists, altering patterns of hormone synthesis, hormone receptor affinities or numbers, and modulating enzymes involved in hormone secretion. These reports highlight the pleiotropic nature of PCB function and the susceptibility of the reproductive system. Endocrine-disrupting PCBs can mimic, alter, or block hormonal responses, inhibiting natural signaling to the testes and epididymis via various mechanisms such as binding to sex hormone-binding globulin and androgen-binding protein or blocking cell surface receptors. Furthermore, PCBs can alter the hormonal environment in the prostate or seminal vesicles by changing the affinity of androgens for their receptors. The testicles and genital organs may be susceptible to various estrogenic effects, leading to changes in the quality or quantity of their secretions and the volume of semen.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"537-554"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of Collagen Tissue Scaffolds in 3D Endometrial-like Culture Systems: Important Contributions to Cell Invasion and Cell Topography.

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI: 10.1007/s43032-025-01800-2

Remziye Kendirci-Katirci, Aylin Sendemir, Elif Esin Hameş, H Seda Vatansever

{"title":"The role of Collagen Tissue Scaffolds in 3D Endometrial-like Culture Systems: Important Contributions to Cell Invasion and Cell Topography.","authors":"Remziye Kendirci-Katirci, Aylin Sendemir, Elif Esin Hameş, H Seda Vatansever","doi":"10.1007/s43032-025-01800-2","DOIUrl":"10.1007/s43032-025-01800-2","url":null,"abstract":"Considering the similarity between the invasion processes of cancer cells and embryo implantation, three-dimensional culture models used to study cancer cell invasion can also be applied to embryo implantation studies. In our study, endometrial epithelial cell line (RL95-2) and spheroid-forming trophoblast-like choriocarcinoma cell line (JAR) were cultured on three different biocompatible tissue scaffolds: bacterial cellulose, collagen foam and collagen fibre. These scaffolds are frequently used in cancer cell metastasis and invasion studies, A three-dimensional endometrium-like culture system was established to quantitatively investigate the role of E-cadherin, N-cadherin, Vimentin, α-smooth muscle actin and Syndecan-1 proteins in the type 1 epithelial mesenchymal transition mechanism observed during the invasion step of the implantation process. Based on the findings from the three-dimensional cell culture, the bacterial cellulose scaffold promoted the proliferation of RL95-2 cells and delayed JAR spheroid formation. The collagen foam scaffold favored the proliferation of RL95-2 cells and accelerated JAR spheroid formation. The collagen fibre scaffold is important for supporting cell topography and, when combined with collagen foam, may offer a potential solution for investigating 3D endometrium-like culture systems. Immunocytochemical and immunofluorescence analyses showed that scaffolds modulate the invasion process by affecting the expression of epithelial mesenchymal transition proteins in cells. The findings suggest that different tissue scaffolds can produce varying effects in endometrium-like culture systems, and combinations of these materials may yield more effective results in future studies. This research represents a critical step in studying cell behavior in 3D culture systems and elucidates the mechanism of endometrial invasion.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"895-906"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0