Reproductive Sciences最新文献_第7页

Effects of Testosterone on Cumulus Cells Gene Expression in Patients with Diminished Ovarian Reserve. 睾酮对卵巢储备功能减退患者积云细胞基因表达的影响。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-06-30 DOI: 10.1007/s43032-025-01906-7

Bruno Tarasconi, Tatiana C S Bonetti, Dieli Primo, Eduardo L A Motta

{"title":"Effects of Testosterone on Cumulus Cells Gene Expression in Patients with Diminished Ovarian Reserve.","authors":"Bruno Tarasconi, Tatiana C S Bonetti, Dieli Primo, Eduardo L A Motta","doi":"10.1007/s43032-025-01906-7","DOIUrl":"10.1007/s43032-025-01906-7","url":null,"abstract":"Diminished ovarian reserve represents a challenging condition in assisted reproductive technology. Androgens' actions are recognized as instrumental in folliculogenesis based on animal and human studies. Thus, we aimed at investigating whether testosterone pretreatment has an influence on gene expression of cumulus cells of diminished ovarian reserve patients. We included patients under 42 years-old presenting for IVF with diminished ovarian reserve (antral follicle count ≤ 6 and antimüllerian hormone < 1.2 ng/ml), between may/2018 and august/2019. Stratified randomization was performed and subjects were allocated into testosterone group (receiving transdermal testosterone gel pretreatment 1% 25 mg daily; N = 9) or control group (receiving standard ovarian stimulation; N = 9). Cumulus cells were collected following oocyte retrieval and RT-qPCR was conducted to evaluate gene expression differences in members of AKT signaling pathway, mTOR signaling pathway, IGF1, FSHR and AMH. Testosterone group patients showed a lower expression of AKT1 in cumulus cells as compared to control group subjects. Other evaluated target genes of the same pathway (FOXO1, FOXO3, PTEN), mTOR signaling pathway genes (mTOR, TSC1, TSC2), as well as IGF1, FHSR and AMH displayed no significant differences between groups. Testosterone pretreatment in diminished ovarian reserve patients down-regulates AKT1 gene expression in cumulus cells. The described profile could be linked to better oocyte reproductive potential, as cumulus cells related to competent oocytes might decide their fate (apoptosis) after completing their role in sustaining oocyte development during cyclic follicular recruitment. Transdermal testosterone pretreatment in diminished ovarian reserve patients down-regulates AKT1 in cumulus cells, possibly enhancing oocyte reproductive potential.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2633-2643"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Machine Learning Model for Predicting Natural Conception Using Non-Laboratory-Based Data. 利用非实验室数据预测自然概念的新型机器学习模型。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-07-14 DOI: 10.1007/s43032-025-01927-2

Yeliz Kaya, Yunus Aydın, Coşkun Kaya, Tuğba Tahta, Özer Çelik

{"title":"A Novel Machine Learning Model for Predicting Natural Conception Using Non-Laboratory-Based Data.","authors":"Yeliz Kaya, Yunus Aydın, Coşkun Kaya, Tuğba Tahta, Özer Çelik","doi":"10.1007/s43032-025-01927-2","DOIUrl":"10.1007/s43032-025-01927-2","url":null,"abstract":"This study aimed to predict the likelihood of natural conception among couples by using a machine learning (ML) approach based on sociodemographic and sexual health data. This marks a novel, non-invasive methodology for fertility prediction. This prospective study included 197 couples divided into two groups: 98 fertile couples (Group 1) who achieved natural conception within one year, and 99 infertile couples (Group 2) who were unable to conceive despite regular unprotected intercourse. Data were collected using a structured form capturing 63 variables from both partners. Using the Permutation Feature Importance method, 25 key predictors were selected. The variables included BMI, age, menstrual cycle characteristics, and varicocele presence. Five ML models were developed and their performance was evaluated using metrics such as accuracy, sensitivity, and specificity. The XGB Classifier showed the highest performance among the models tested with an accuracy of 62.5% and a ROC-AUC of 0.580, indicating limited predictive capacity. The selected predictors encompassed a balance of medical, lifestyle, and reproductive factors for both partners, emphasizing the couple-based approach. Key factors included BMI, caffeine consumption, history of endometriosis, and exposure to chemical agents or heat. This study assessed the use of ML to predict natural conception using sociodemographic and health data The key predictors identified emphasize the importance of couple-based and lifestyle factors in predicting natural conception. However, the predictive capacity of the models was limited, highlighting the need for future studies with larger datasets and expanded predictors to improve accuracy and facilitate AI integration into fertility assessment.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2644-2653"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

YTHDC1 Promoted Cell Proliferation and Decidualization by Maintaining Nuclear C/EBPβ Stability in Decidual Stromal Cells. YTHDC1通过维持细胞核C/EBPβ在蜕质细胞中的稳定性来促进细胞增殖和蜕质化。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-06-04 DOI: 10.1007/s43032-025-01888-6

Weihua He, Yating Zhao, Lijun Yin, Jianhua Qian

{"title":"YTHDC1 Promoted Cell Proliferation and Decidualization by Maintaining Nuclear C/EBPβ Stability in Decidual Stromal Cells.","authors":"Weihua He, Yating Zhao, Lijun Yin, Jianhua Qian","doi":"10.1007/s43032-025-01888-6","DOIUrl":"10.1007/s43032-025-01888-6","url":null,"abstract":"m6A modification has been shown to play a role in regulating female reproductive diseases. The YT521-B homology (YTH) domain family, including the m6A readers YTHDC1 and YTHDC2, is associated with decidualization during pregnancy. This study aimed to investigate the role of YTHDC1/2-regulated m6A modification in decidualization of endometrial stromal cells (ESCs). We found that YTHDC1 and YTHDC2 were highly expressed in decidual tissues from normal pregnancies. Knockdown of YTHDC1 suppressed cell proliferation and decidualization marker expression in decidual stromal cells (DSCs), whereas overexpression of YTHDC1 enhanced these processes. Mechanistically, YTHDC1 interacted with C/EBPβ via m6A modification and targeted its 3'UTR region. Knockdown of YTHDC1 accelerated the degradation of nuclear C/EBPβ mRNA in DSCs but had minimal effect on cytoplasmic C/EBPβ. Overexpression of C/EBPβ partially rescued the shYTHDC1-induced suppression of cell proliferation and decidualization markers. These results demonstrate that YTHDC1 promotes decidualization and proliferation in DSCs by stabilizing nuclear C/EBPβ mRNA through m6A modification, suggesting a novel therapeutic target for decidualization-related reproductive disorders.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2779-2792"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between Lactobacillus of Vaginal Microbiota and the Pregnancy Outcomes for Patients Experiencing Recurrent Miscarriage. 阴道菌群乳酸杆菌与复发性流产患者妊娠结局的关系。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-04-22 DOI: 10.1007/s43032-025-01869-9

Jifan Tan, Ruyun Chen, Dehai Gan, Miaoxian Ou, Yue Wu, Huiying Jie, Yanwen Xu, Jia Huang

{"title":"Correlation between Lactobacillus of Vaginal Microbiota and the Pregnancy Outcomes for Patients Experiencing Recurrent Miscarriage.","authors":"Jifan Tan, Ruyun Chen, Dehai Gan, Miaoxian Ou, Yue Wu, Huiying Jie, Yanwen Xu, Jia Huang","doi":"10.1007/s43032-025-01869-9","DOIUrl":"10.1007/s43032-025-01869-9","url":null,"abstract":"The etiology of recurrent miscarriage (RM) is complex, with the vaginal microbiota (VM) being an important factor associated with RM. We aimed to establish the VM composition in both patients with RM and healthy women and further investigate relationship between the subsequent pregnancy outcomes of patients with RM and VM, to explain the potential mechanism of VM in RM to some extent. A cohort study compared the VM between 34 patients with RM and 15 healthy women using a sequencing technique based on Type IIB restriction enzymes for the microbiome (2bRAD-M). Further comparison was made between 11 patients with clinical miscarriages (CM) and 13 patients with ongoing pregnancies (OP) in the RM group who conceived naturally. To determine the VM composition, the 2bRAD-M library was prepared, and sequence and bioinformatics analyses were conducted. The composition of the VM exhibited notable differences between the non-RM and RM groups, with significant findings for alpha diversity (p < 0.05) and beta diversity (p = 0.01). Further analysis between the RM-OP and RM-CM groups revealed a significant difference in Lactobacillus (97.81% ± 2.71% vs. 53.37% ± 46.42%, p = 0.03). Other uncommon species, such as Cutibacterium acnes (C. acnes) (p = 0.04) were found significantly increase in the RM-CM group. Functional annotation analysis revealed 47 related signaling pathways between the two groups. The results of this study indicate that Lactobacillus is associated with subsequent miscarriages and that C. acnes is closely related to pregnancy outcomes of patients with RM.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2729-2741"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Ambient Ozone Pollution Exposure on Mice Testicular Tissue: An Integrated Metabolomic and Transcriptomic Analysis. 环境臭氧污染暴露对小鼠睾丸组织的评价：综合代谢组学和转录组学分析。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-06-23 DOI: 10.1007/s43032-025-01907-6

Xiaoqin Feng, Jinhong Luo

{"title":"Evaluation of Ambient Ozone Pollution Exposure on Mice Testicular Tissue: An Integrated Metabolomic and Transcriptomic Analysis.","authors":"Xiaoqin Feng, Jinhong Luo","doi":"10.1007/s43032-025-01907-6","DOIUrl":"10.1007/s43032-025-01907-6","url":null,"abstract":"The real ozone concentration in the air is rising yearly globally, which remarkably has adverse effect on human health, particularly on male fertility. Epidemiological studies showed that men exposure to ambient ozone was linked with decreased semen quality. Therefore, it is urgent to elucidate the toxicological evaluation of ozone exposure on men reproduction. This study conducted transcriptomic mRNA sequencing and high-performance liquid chromatography-tandem mass spectrometry to depict the characteristics of metabolomics and transcriptomics of mice testis tissue exposed to ozone. In transcriptomic, KEGG showed that spermatogenesis was significantly influenced by ozone exposure, meanwhile dysregulation of protein serine/threonine kinase activity, intracellular signal transduction, cell projection organization, and regulation of microtubule polymerization were observed, these molecular events participate in regulation and control of spermatogenesis. In metabolomic, twenty differential metabolites were the top abundance ranking features, as intermediate products of phospholipid metabolism to act as signal molecules, participating in intracellular signal transduction pathways, may affect the fluidity and structure of sperm cell membrane. Integrated omics showed that aminoacyl-tRNA biosynthesis was the main pathway attributed to significant correlation between transcriptomics and metabolomics (combination p value = 0.01), including arginine biosynthesis, essential for spermatogenesis. In integrated omics, several signatures were identified to be associated with spermatogenesis, including Creb3l4, Pgp and Tcp1, contributing to maturation of sperm head nuclei, energy metabolism and formation of sperm flagellum. The finding suggested ambient ozone exposure significantly altered the characteristics of transcriptome and metabolome of mice testis, and impaired spermatogenesis. Our finding provided new evidence of more stringent control and management for ambient ozone so as to reduce the potential risk of declined male fertility.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2802-2818"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Transcriptome and RNA Sequencing Reveal Immune-Related Markers of Preeclampsia. 单细胞转录组和RNA测序揭示子痫前期免疫相关标志物。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-04-11 DOI: 10.1007/s43032-025-01843-5

Xiaoting Yu, Huiqiu Xiang, Xianping Huang

{"title":"Single-Cell Transcriptome and RNA Sequencing Reveal Immune-Related Markers of Preeclampsia.","authors":"Xiaoting Yu, Huiqiu Xiang, Xianping Huang","doi":"10.1007/s43032-025-01843-5","DOIUrl":"10.1007/s43032-025-01843-5","url":null,"abstract":"Preeclampsia is a leading cause of maternal and fetal mortality, posing a threat to the mother and fetus's lives, but its pathogenesis is not yet clear. This study aimed to find the key target genes regulating preeclampsia in the placental immune microenvironment through single-cell and RNA-sequencing. This study compared the immune microenvironment of the placenta in preeclamptic and non-preeclamptic samples. Gene Ontology Enrichment (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), pseudotime, and cell-cell communication techniques were utilized to analyze the association between immune cell function and the pathogenesis of preeclampsia. Specific transcription factor target genes of immune cells were obtained based on the Scenic package and intersections were taken with their differentially expressed genes (DEGs). Key differential genes analysis was performed on the intersected genes based on the GSE234729 and GSE25906 datasets to obtain differential target genes regulating preeclampsia in immune cells. A total of 10 cell types were annotated in the placenta. Among them, macrophages had the highest immune score, followed by monocytes. GO and KEGG results demonstrated that they might be involved in inflammatory response and vascular remodeling in preeclampsia. Macrophages in the placenta were reclustered and annotated to monocytes, macrophages, and Hofbauer cell subsets, of these, monocytes could differentiate into macrophages and Hofbauer cells. Of all the immune cell-regulated preeclampsia differential genes SLC9A9, SH2B3, SDC3, RCC2, F13A1, CCL2, and CBLB were consistently expressed in two transcriptome datasets, and all were highly expressed in macrophages. These findings suggested that macrophages were implicated in the aberrant immune and inflammatory response of the preeclamptic placenta and found its key target genes that regulate preeclampsia.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2819-2828"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remifentanil Attenuates LPS-Induced Genital Tract Injury by Modulating Inflammation, Oxidative Stress, and Mitochondrial Gene Expression in a Rat Sepsis Model. 在大鼠脓毒症模型中，瑞芬太尼通过调节炎症、氧化应激和线粒体基因表达减轻lps诱导的生殖道损伤。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-07-15 DOI: 10.1007/s43032-025-01930-7

Ozlem Ozmen, Halil Asci, Senay Topsakal, Rumeysa Taner, Mert Oktem, Kadriye Nilay Ozcan, Bunyamin Aydin

引用次数: 0

Melatonin Rescues Hyperacetylation of Liver and Impaired Enzymatic Activities of Mitochondrial in IVF Offspring. 褪黑素恢复体外受精后代肝脏乙酰化和线粒体酶活性受损。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-04-17 DOI: 10.1007/s43032-025-01846-2

Yanping Jia, Mingming Ye, Orhan Bukulmez, Robert J Norman, Wenqiang Liu, Miaoxin Chen

{"title":"Melatonin Rescues Hyperacetylation of Liver and Impaired Enzymatic Activities of Mitochondrial in IVF Offspring.","authors":"Yanping Jia, Mingming Ye, Orhan Bukulmez, Robert J Norman, Wenqiang Liu, Miaoxin Chen","doi":"10.1007/s43032-025-01846-2","DOIUrl":"10.1007/s43032-025-01846-2","url":null,"abstract":"Increased risks of obesity and abnormal glucose metabolism were observed in IVF offspring. However, the underlying molecular mechanism was still unclear. As an important post-translational modification (PTM), lysine acetylation changed with the changes in the metabolic environment and usually occurred on metabolic enzymes to regulate metabolic pathways and enzyme activities and participated in the regulation of downstream metabolites. In our previous study, we proved that supplementation of melatonin in the culture medium improved obesity and metabolic dysfunction in IVF mice. In this study, we further demonstrated that elevated levels of protein acetylation in hepatic cells might be associated with impaired glucose metabolism in IVF offspring, and melatonin could significantly reduce the acetylation level and improve the adverse phenotype of IVF mice. More importantly, we discovered that the supplementation of melatonin in the culture medium during in vitro fertilization significantly enhanced the activity of enzymes, especially citrate synthase (CS) and isocitrate dehydrogenase (IDH) which were involved in tricarboxylic acid recycling and played critical roles in glucose metabolism of liver. Thus, our findings elucidated a new perspective on the mechanisms of metabolic reprogramming of IVF mice.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2717-2728"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of hub genes and potential molecular mechanisms of tumor immune microenvironment-related radiotherapy sensitivity in locally advanced cervical cancer. 局部晚期宫颈癌肿瘤免疫微环境相关放疗敏感性枢纽基因的鉴定及潜在分子机制

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-06-30 DOI: 10.1007/s43032-025-01909-4

Yichen Zan, Qing Liu, Yue Yin, Chongxin Guo, Jing Xu, Junhan Gao, Guanglu Dong

{"title":"Identification of hub genes and potential molecular mechanisms of tumor immune microenvironment-related radiotherapy sensitivity in locally advanced cervical cancer.","authors":"Yichen Zan, Qing Liu, Yue Yin, Chongxin Guo, Jing Xu, Junhan Gao, Guanglu Dong","doi":"10.1007/s43032-025-01909-4","DOIUrl":"10.1007/s43032-025-01909-4","url":null,"abstract":"Cervical cancer remains the most prevalent malignancy of the female reproductive system; however, the five-year survival rate has not improved substantially over recent decades. This persistent challenge highlights the critical need to elucidate mechanisms underlying treatment sensitivity and investigate synergistic effects of multimodal therapies to overcome clinical therapeutic bottlenecks. By conducting a comprehensive analysis of The Cancer Genome Atlas (TCGA) database, this study identified a hub gene network associated with radiotherapy sensitivity in cervical cancer. In addition to developing a personalized treatment prediction model, we preliminarily characterized these hub genes through several key dimensions: molecular regulatory, biological functions, tumor microenvironment infiltration patterns, and immune relevance. Notably, this work is the first to reveal that hub genes such as DDX58 probably orchestrate a tripartite regulatory mechanism-mediated by immune microenvironment factors-that concurrently influences sensitivity to radiotherapy, chemotherapy, and immunotherapy. Through a series of analyses, we speculate that these hub genes can serve not only as combined biomarkers for predicting responses to multiple therapies but also as novel molecular targets for the development of synergistic treatment strategies via their mediated immune microenvironment interactions. These findings provide a new translational research direction for overcoming therapeutic resistance in cervical cancer.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2607-2632"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracytoplasmic Sperm Injection (ICSI) Timing Affects the Influence of Sperm DNA Fragmentation on ICSI Outcomes. 卵胞浆内单精子注射（ICSI）时机影响精子DNA片段化对ICSI结果的影响。

IF 2.5 3区医学

Reproductive Sciences Pub Date : 2025-08-01 Epub Date: 2025-07-01 DOI: 10.1007/s43032-025-01924-5

Gaofeng Yan, Dan Tang, Zhenfang Su, Longda Wang, Youlin Yao, Wenjuan Yang, Ling Lu, Xiufang Gui, Huawei Wang, Shuhua Zhao

{"title":"Intracytoplasmic Sperm Injection (ICSI) Timing Affects the Influence of Sperm DNA Fragmentation on ICSI Outcomes.","authors":"Gaofeng Yan, Dan Tang, Zhenfang Su, Longda Wang, Youlin Yao, Wenjuan Yang, Ling Lu, Xiufang Gui, Huawei Wang, Shuhua Zhao","doi":"10.1007/s43032-025-01924-5","DOIUrl":"10.1007/s43032-025-01924-5","url":null,"abstract":"Oocytes have repair ability for paternally derived DNA damage which depends on oocyte quality. Prolonged post ovum pick-up (OPU) incubation may lead to oocyte aging, which is linked to changes in oocyte ultrastructure and function. In order to study whether the effect of sperm DNA fragmentation (SDF) on intracytoplasmic sperm injection (ICSI) outcomes influenced by ICSI timing, we conducted this retrospective study included 1,331 ICSI cycles from March 2016 to December 2022. Outcomes were compared between two groups (an SDF level < 30% group and an SDF level ≥ 30% group) at different tertiles, which were based on OPU-ICSI time. There were no significant between-group differences in the overall laboratory and clinical outcomes (all p > 0.05). However, the clinical pregnancy rates (CPRs) (28.6% vs. 40.9%, p = 0.017) and live birth rates (LBRs) (21.4% vs. 33.4%, p = 0.014) were significantly lower in the SDF level ≥ 30% group than in the SDF level < 30% group in tertile 3. After adjusting for confounders, these differences remained significant, as shown by odds ratios (ORs) (95% confidence intervals (CIs)) of 0.425 (0.244, 0.742) for CPR and 0.485 (0.272, 0.865) for LBR. Significant between-tertile differences in outcomes were only found in the SDF level ≥ 30% group. In conclusion, ICSI timing affects the influence of SDF on the probability of live birth in women undergoing ICSI treatment. Therefore, in patients with high SDF levels, the optimal timing of ICSI should be determined.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"2692-2702"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144542053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0