Reproductive Sciences最新文献

{"title":"miR-1264 Exacerbates Proliferation, Migration and Invasion of Endometrial Cancer Cells by Targeting MSH2.","authors":"Wen Han, Xiang Yong, Bei Wang, Qian Zhang, Yi Zhang, Mingyu Shao, Chun Wang","doi":"10.1007/s43032-025-01814-w","DOIUrl":"https://doi.org/10.1007/s43032-025-01814-w","url":null,"abstract":"<p><p>Accumulating studies have revealed that microRNAs serve significant regulatory for endometrial carcinoma (EC) tumorigenesis and progression. The specific objective of this investigation is to seek the potential function of miR-1264 in EC and clarified the underlying mechanism. Determination of miR-1264 and MSH2 expression in EC tissues or cell lines was performed by RT-qPCR and/or western blot assay. The malignant behaviors of EC cells were verified by CCK-8 assay, EdU staining, wound healing assay and Transwell assay, respectively. Besides, the interaction between miR-1264 and MSH2 was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay. MiR-1264 exhibited high levels in EC tissues and has been found to be correlated with a poor prognosis in EC patients. Functionally, silencing miR-1264 resulted in inhibiting the aggressiveness behaviors of HEC-1 A and KLE cells, while forced miR-1264 expression executed opposite effects. Mechanistically, miR-1264 directly targeted and suppressed MSH2 expression. Functional rescue experiments further validated that overexpression of MSH2 diminished malignant behaviors of EC cells and greatly reversed the promoting effects of miR-1264 on the malignant behaviors of EC cells. MiR-1264 acted as an oncogene in EC, promoting aggressiveness behaviors of EC cells by inhibiting MSH2. This study provided novel insights into anti-cancer treatment and a theoretical basis for potential therapeutic targets of EC.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in the Hippo Signaling Pathway During Adenogenesis Impairment in Postnatal Mouse Uterus.","authors":"İrem İnanç, Onur Bender, Arzu Atalay, Serdal Kenan Köse, Esra Erdemli","doi":"10.1007/s43032-025-01793-y","DOIUrl":"https://doi.org/10.1007/s43032-025-01793-y","url":null,"abstract":"<p><p>The mouse uterus, which consists of single-layered epithelium and undifferentiated mesenchyme at birth, begins to differentiate in the postnatal period. The process of adenogenesis, defined as gland development, begins on the Postnatal (PN) Day 5, and this process is very evident on the PN Day 10. Although various signaling pathways effective in the adenogenesis process but the mechanism underlying this progress have not been clarified yet. Hippo signaling pathway have roles in many cellular functions, such as proliferation, differentiation and cell death. But the relationship between the Hippo signaling pathway and uterus adenogenesis is unknown. The objective of this study has been to determine if there is a change in the Hippo signaling pathway in mice with impaired gland development during the adenogenesis process. To that aim, we use mouse uterus with normal gland development (control group) and gland development inhibited by progesterone (experimental group). Animals were sacrificed on the PN Days 5, 10 and 15. YAP and p-YAP by immunohistochemistry and immunoblotting techniques to identify the main components of Hippo Signaling Pathway. YAP, LATS1, LATS 2, MST1, NF2 and TAZ used for the RT-qPCR methods. In conclusion, Hippo signaling pathway components were reduced during the adenogenesis process in mouse with impaired gland development.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Various Mechanisms by Which Exercise-Induced Fatigue (EIF) Affects Spermatogenesis Through Testosterone.","authors":"Kun-Yang Tang, Zhi-Peng Fang, Min Xiao","doi":"10.1007/s43032-025-01804-y","DOIUrl":"https://doi.org/10.1007/s43032-025-01804-y","url":null,"abstract":"<p><p>Currently, a great number of researches have proven that the EIF triggered by inappropriate and excessive exercise will affect normal function of the male reproductive system, resulting in various disorders or increasing disease risks. Reproductive system and motor system are highly correlated. Now, it is mainly considered that the testicular cell is modulated via hypothalamus-hypophysis-gonad(HPG) axis in the process of exercise, which involves the whole male reproductive system and affects spermatogenesis. However, there are many mechanisms leading to EIF, including hormonal levels, lactic acid accumulation, oxidative stress, calcium ion disorder, and inflammatory responses. These mechanisms interact with each other and can directly or indirectly affect spermatogenesis through testosterone hormones in various ways, with the majority having a negative impact. This ultimately leads to dysfunction of spermatogenesis and decreased sperm quality in the male reproductive system under the state of EIF. Therefore, this article reviews various theoretical mechanisms leading to EIF and their intervention methods on spermatogenesis through testosterone hormones, explaining the correlation between EIF and spermatogenesis from the perspective of endocrine regulation, providing new directions for the prevention and treatment of related symptoms.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat Stress and Its Impact on Corpus Luteum (CL) Function and Reproductive Efficiency in Mammals: A Critical Review.","authors":"Muhammad Tariq, Saba Saeed, Kossinga Koulet Andre Saint Victor, Arooj Fatima, Dagan Mao","doi":"10.1007/s43032-025-01787-w","DOIUrl":"https://doi.org/10.1007/s43032-025-01787-w","url":null,"abstract":"<p><p>Heat stress is considered as one of the most crucial environmental stressors affecting reproductive efficiency in mammals through modulation of the function of Corpus Luteum (CL) that plays a vital role in progesterone production and pregnancy maintenance. Therefore, this detailed systematic review seeks to bring forward the interdisciplinary perspectives on the impact of heat stress exposure on CL function regarding hormonal shift, luteal phase distortion and fertility receptivity. High temperatures are shown to impose oxidative stress, change blood perfusion within the CL, signal transduction which converts the signal from the signaling molecule into an intracellular reaction and impaired luteal activity. This review incorporates various scientific studies on these effects to different mammalian species concerning the associated physiological mechanisms. Besides this, it also considers the overall impact in warm stressed population in livestock breeding in the agricultural system as well as their conservation from a general perspective. Some of the prevention and control measures for heat related reproductive problems are also covered here, addressing the importance of finding the impact on the CL in order to put in place these interventions. This review may be used to inform future developments that may improve the CL function with regards to heat stress and possible solutions to help mammals reproduced under climate change tender environment and even rising temperatures globally.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hysteroscopic Endometrial Defect Following Adenomyomectomy and Incidence of Placenta Accreta Spectrum and Uterine Rupture Complications for Subsequent Pregnancy.","authors":"Mari Ichinose, Takayuki Iriyama, Osamu Hiraike, Seisuke Sayama, Ayako Hashimoto, Kensuke Suzuki, Mitsunori Matsuo, Masatake Toshimitsu, Takahiro Seyama, Kenbun Sone, Keiichi Kumasawa, Yasushi Hirota, Yutaka Osuga","doi":"10.1007/s43032-024-01758-7","DOIUrl":"10.1007/s43032-024-01758-7","url":null,"abstract":"<p><p>Adenomyomectomy, a therapeutic option for women with adenomyosis who wish to preserve their fertility, has been reported to pose a risk of developing placenta accreta spectrum (PAS) and uterine rupture in future pregnancies. However, the specific clinical factors contributing to these occurrences remain elusive. This study aimed to explore the association between hysteroscopic findings after adenomyomectomy and the incidence of PAS in subsequent pregnancies. We conducted a retrospective analysis of 10 patients (11 pregnancies) who had undergone hysteroscopy following adenomyomectomy and had later delivered at our hospital. In 6/10 patients, postoperative hysteroscopy revealed endometrial defects. However, subsequent evaluations confirmed endometrial restoration within 7-21 months, with five patients achieving pregnancy afterward. The only other patient conceived naturally without waiting for endometrial restoration, resulting in uterine rupture from the site of the placenta percreta. The incidence of clinically diagnosed PAS during cesarean section was 100% (1/1) in pregnancies with preconceptional endometrial defects, 20% (1/5) in those with endometrial restoration, and 0% (0/5) in pregnancies without endometrial defects. Similarly, the incidence of pathologically diagnosed PAS was 100% (1/1), 60% (3/5), and 20% (1/5) in these groups, respectively. Thus, endometrial defects were frequently detected after adenomyomectomy and recovered over time, whereas one patient without endometrial restoration developed uterine rupture complicated by PAS. This study demonstrates that while the presence of an endometrial defect identified by postoperative hysteroscopy may be a risk factor for the occurrence of PAS in subsequent pregnancies, allowing sufficient recovery time for the endometrium may help reduce the risk of uterine rupture.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"467-473"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Manipulation of Spermatogonial Stem Cells with the Aim of Inducing Spermatogenesis in Vitro.","authors":"Banafsheh Heidari, Abolfazl Shirazi, Nazanin Akbari, Maral Barzegar-Amini","doi":"10.1007/s43032-024-01709-2","DOIUrl":"10.1007/s43032-024-01709-2","url":null,"abstract":"<p><p>Assisted reproduction techniques for infertile men with non-obstructive azoospermia require a sufficient number of functional germ cells produced in vitro. Understanding the mechanisms that allow the resumption of spermatogenesis outside the testicular environment is crucial for fertility preservation in these patients. A review of the literature was conducted using databases such as PubMed, Scopus and Web of Science, with keywords including \"spermatogonial stem cell,\" \"germ cells,\" \"male factor infertility,\" and \"enrichment and propagation of SSCs in vitro.\" Currently, two models-\"in vivo\" and \"in vitro\"-have been developed for producing haploid germ cells. The \"in vivo\" models include spermatogonial stem cell transplantation and testicular xenograft techniques. In contrast, the \"in vitro\" models consist of conventional culture systems, organ culture, and three-dimensional culture systems, all designed to induce spermatogenesis in vitro. These culture systems enable the simulation of the seminiferous epithelium in vitro, which facilitates better regulation of cell-signaling pathways that control the self-renewal and differentiation of SSCs. This review provides up-to-date information on the organization of SSCs, focusing on the identification, proliferation, and differentiation of spermatogonia in vitro.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"278-288"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Hypoxia to Oxidative Stress: Antioxidants' Role to Reduce Male Reproductive Damage.","authors":"Siyao Li, Wenjing Liu, Xin Chen, Zhaoyu Chen, Jingtian Shi, Juan Hua","doi":"10.1007/s43032-024-01746-x","DOIUrl":"10.1007/s43032-024-01746-x","url":null,"abstract":"<p><p>Hypoxia is one of the main reasons causing male reproductive damage for people living in high altitude. Pathological evidences have been presented both in humans and animal models. Spermatogenesis disruption, worse sperm parameters, hormone disorder and erectile dysfunction are emblematic of male reproductive impairments brought by hypoxia. Among many mechanisms impairing male reproductive systems, oxidative stress is always a field of interest to explore. Although previous reviews have discussed about hypoxia or oxidative stress and antioxidants on male fertility respectively, no one has elucidated the concrete role of oxidative stress in hypoxia and correlating antioxidants that can ameliorate the negative effects. In this review, we firstly introduce hypoxia etiology and describe specific damage of hypoxia on male reproductive functions. Then, we emphasized interplays between hypoxia and oxidative stress as well as negative influences brought by oxidative stress. Finally, we listed antioxidants for oxidative stress and hypoxia-induced reproductive damage and discussed their controversial experimental effects for male infertility.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"261-277"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Alterations Expression of Key RNA Methylation (m6A) Enzymes in Testicular Tissue of Rats with Induced Varicocele.","authors":"Mohammad Shojaei, Marziyeh Tavalaee, Bahareh Ghazavi, Tayebeh Izadi, Zahra Safaeinejad, Elham Ghajari, Ali Valipour Motlagh, Mohammad H Nasr-Esfahani","doi":"10.1007/s43032-024-01751-0","DOIUrl":"10.1007/s43032-024-01751-0","url":null,"abstract":"","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"535"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Microbiome Landscape of Adenomyosis: A Systematic Review.","authors":"Michelle D Dantzler, Tiffani-Amber Miller, Michael W Dougherty, Amira Quevedo","doi":"10.1007/s43032-024-01766-7","DOIUrl":"10.1007/s43032-024-01766-7","url":null,"abstract":"<p><p>Alterations in the microbiome composition have been identified in common gynecologic pathologies such as endometriosis carving a new frontier in diagnosis and treatment. We aimed to examine the existing literature on perturbations in the reproductive tract microbiome of individuals with adenomyosis informing future therapeutic targets. To examine the association between the reproductive tract microbiome composition among individuals with adenomyosis when compared to controls that can lead to new research evaluating novel mechanisms of action and treatment modalities. A systematic literature search identified studies that compared differences in microbiome composition using culture-independent microbiome analysis between individuals with adenomyosis when compared to controls. Five observational cross-sectional studies characterizing the lower and upper reproductive tract in humans were included. The diagnostic criteria of adenomyosis included surgical and imaging-based criteria. All studies used a 16S rRNA sequencing method. All individuals were recruited from either China or Thailand. An association between adenomyosis and alterations in the microbiome composition included relative deficiencies in Lactobacillus and relative enrichment of anaerobic and gram-negative bacteria when compared to control participants. Comparative studies suggest that there are significant perturbations in the microbiota composition of individuals with adenomyosis when compared with controls. Limiting conclusions include relative small sample sizes, a homogeneous population, and scant clinical phenotypic data. This systematic review identified significant alterations in the bacterial composition of adenomyosis cases that can be leveraged to design mechanistic studies and future innovative approaches to diagnose and manage this pathology. Trial registration: PROSPERO (CRD42023494563). Registered December 28, 2023.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"251-260"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluoxetine Mitigates Human Sperm Quality by Disrupting the Antioxidant Defense System and Altering the Expression of Apoptosis-Related Genes: An In Vitro Study.","authors":"Zahra Roostaee, Malek Soleimani Mehranjani, Ebrahim Cheraghi","doi":"10.1007/s43032-024-01760-z","DOIUrl":"10.1007/s43032-024-01760-z","url":null,"abstract":"<p><p>Fluoxetine is used in the management of depression, anxiety and other mood disorders by increasing serotonin levels in the brain and can cause sexual side effects by changing the homeostasis of sex hormones and increasing oxidative stress. Since many men who take fluoxetine are of reproductive age and sperm are exposed to fluoxetine for a considerable time, this study aimed to examine the in vitro effects of fluoxetine on human sperm biochemical markers and sperm parameters. Semen samples from 30 fertile men were divided into three groups: a positive control group, a negative control group and a fluoxetine-treated group. We investigated sperm parameters, mitochondrial membrane potential (MMP), acrosome reaction, DNA fragmentation, chromatin integrity, and the expression of CASPASE8, CASPASE9, BAX, and BCL2 genes and proteins. Data were analyzed using repeated measures analysis. The results showed that the average percentage of motility, viability, MMP, acrosome and chromatin integrity, total antioxidant capacity (TAC) level, and BCL2 gene and protein expression in the fluoxetine group were significantly reduced compared to the positive and negative control groups. While the average percentage of non-progressive motility, sperm DNA fragmentation, malondialdehyde (MDA) level, reactive oxygen species (ROS), gene and proteins expression of CASPASE8, CASPASE9 and BAX increased significantly. This study suggests that fluoxetine may impair sperm quality by increasing the expression of apoptotic genes, proteins, and oxidative stress. Therefore, careful management of fluoxetine in treating depression is crucial, especially in men of reproductive age, due to its potential sexual side effects. HIGHLIGHTS: • Fluoxetine reduces the quality of human sperm by inducing oxidative stress. • Fluoxetine lowers the total antioxidant capacity in human sperm by increasing ROS. • Fluoxetine increases the expression of apoptosis genes in human sperm.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"326-342"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142954093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}