Reproductive Sciences最新文献_第8页

Exploring the Therapeutic Potential of Terminalia chebula Retz. in Alleviating the Complications of Letrozole-Induced PCOS in Rat Model. 探索小儿终叶的治疗潜力。减轻来曲唑致PCOS大鼠模型并发症的研究。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-12 DOI: 10.1007/s43032-025-01813-x

Vignesh Kalimuthu, Swathi Chandran Manimegalai, Ramya Venkatesan, Sathiya Priya Krishnamoorthy, Nigamananda Dey, Thiyagarajan Ramesh, Kadalmani Balamuthu

{"title":"Exploring the Therapeutic Potential of Terminalia chebula Retz. in Alleviating the Complications of Letrozole-Induced PCOS in Rat Model.","authors":"Vignesh Kalimuthu, Swathi Chandran Manimegalai, Ramya Venkatesan, Sathiya Priya Krishnamoorthy, Nigamananda Dey, Thiyagarajan Ramesh, Kadalmani Balamuthu","doi":"10.1007/s43032-025-01813-x","DOIUrl":"10.1007/s43032-025-01813-x","url":null,"abstract":"Polycystic Ovarian Syndrome is a leading gynecological condition that is being known to affect women fertility irrespective of their reproductive age. Though its prevalency and adverse effects in causing the female infertility is know to be reported worldwide, it has the steroidal pills having remarkable side effects as their effective medication to treat and manage its symptoms. Inorder to find a novel curative plant besed therapy, this study investigates the therapeutic potential of Terminalia chebula Retz. to manage the complications of PCOS. In this present study, the bioactive compounds of Terminalia chebula Retz. fruit extract were identified by GC-MS and the experimental animals (female Wistar rats) were categorized into six groups including control, letrozole-induced PCOS group, metformine treated as standard control, along with the groups orally treated with T. chebula fruit extracts at various concentrations. As a result of PCOS induction, the level of LPx got increased evidencing the increased lipid metabolism where the other antioxidant levels were decreased. The serum hormonal profile revealed a considerable decrease in estrogen and progesterone levels while the levels of LH, FSH, testosterone, and insulin were increased. The mRNA and protein expressions of CYP17A1, was upregulated whereas the CYP19A1 and PPAR-γ found to have lower expression on concerning the control group. These entire physiological, and biochemical observed during the successful induction of PCOS got restored to normal after being treated with the fruit extract of T. chebula in the experimental animals and implied its potentiality in managing the complications of PCOS.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"836-853"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical-flow Analysis of the Relationship Between Ooplasmic Fluidity and Oocyte Degeneration During Piezo-intracytoplasmic Sperm Injection. 卵浆内压精子注射过程中卵浆流动性与卵母细胞变性关系的光流分析。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-10 DOI: 10.1007/s43032-025-01807-9

Takashi Morimoto, Hidehiko Matsubayashi, Takumi Takeuchi, Tomomoto Ishikawa, Ryo Haraguchi

{"title":"Optical-flow Analysis of the Relationship Between Ooplasmic Fluidity and Oocyte Degeneration During Piezo-intracytoplasmic Sperm Injection.","authors":"Takashi Morimoto, Hidehiko Matsubayashi, Takumi Takeuchi, Tomomoto Ishikawa, Ryo Haraguchi","doi":"10.1007/s43032-025-01807-9","DOIUrl":"10.1007/s43032-025-01807-9","url":null,"abstract":"To evaluate the predictive ability of ooplasmic fluidity assessment during piezo-intracytoplasmic sperm injection (ICSI) for unintentional membrane rupture (UMR) and oocyte degeneration.We established an optical-flow analysis method that detects ooplasm movement in piezo-ICSI videos. Videos of 104 oocytes recorded during piezo-ICSI at our institute between January 2020 and January 2024 were retrospectively analyzed.To evaluate the apparent velocities in the ooplasmic region, we analyzed the vertical velocity component of the obtained optical flow between the start of puncture and the rupture of the oolemma (1st period) and between sperm injection and pipette withdrawal (2nd period). The velocity distributions between groups were compared using the 95th, 99th, and 99.5th percentiles. The 95th, 99th, and 99.5th percentiles of the survival group (n = 76) were significantly higher than those of the degeneration group (n = 28) during the 1st period (P < 0.001). Similarly, the 95th, 99th, and 99.5th percentiles of the survival group were significantly higher than those of the degenerative group during the 2nd period (P < 0.05). The 95th, 99th, and 99.5th percentiles without UMR (n = 52) were significantly higher than those with UMR (n = 50) during the 1st period (P < 0.001). Similarly, the 95th, 99th, and 99.5th percentiles without UMR were significantly higher than those with UMR during the 2nd period (P < 0.001). Greater ooplasmic fluidity was correlated with lower incidence of UMR and oocyte degeneration. Elucidation of the mechanisms underlying this phenomenon may help reduce UMR and oocyte degeneration.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"684-692"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azurocidin is Associated with Dienogest-resistance in Ovarian Endometriotic Cysts. azuroidine与卵巢子宫内膜异位囊肿的抗药相关。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI: 10.1007/s43032-025-01795-w

Momoe Watanabe, Yoichi Kobayashi, Manami Ishida, Atsushi Tajima, Shinji Tanigaki, Tohru Morisada

{"title":"Azurocidin is Associated with Dienogest-resistance in Ovarian Endometriotic Cysts.","authors":"Momoe Watanabe, Yoichi Kobayashi, Manami Ishida, Atsushi Tajima, Shinji Tanigaki, Tohru Morisada","doi":"10.1007/s43032-025-01795-w","DOIUrl":"10.1007/s43032-025-01795-w","url":null,"abstract":"Endometriosis and ovarian endometrioma (OMA) cause dysmenorrhea and infertility. Current hormonal therapies for OMA treatment, may exhibit limited effectiveness. Hormonal treatments function by downregulate estrogen receptors (ERs) via progesterone receptor (PR) signaling; therefore, progestins are used for the treatment of endometriosis. Dienogest (DNG), an oral progestin, is highly selective for PRs. Previously we identified the association of azurocidin with DNG resistance. Herein, we aimed to examine the effect of azurocidin on OMAs and its clinical significance. We examined the effect of azurocidin on PR or ER and the action of DNG on the inflammatory cytokines IL-6 and IL-8 in OMAs used the human immortalized endometriotic epithelial Emosis-CC/TERT1 cell line, and measured azurocidin levels in human biological samples. DNG inhibited IL-6 and IL-8 production in vitro, which was suppressed in the presence of azurocidin. Additionally, the inflammatory cytokines IL-6 and IL-8 enhanced azurocidin production. Furthermore, azurocidin induced ER expression; the proliferation of EMosis-CC/TERT1 cells increased significantly upon incubation with 17β-estradiol and azurocidin. Overall, azurocidin inhibits the action of DNG by increasing estrogen sensitivity via promoting ER expression and endometriosis. Azurocidin concentrations in the blood and urine were higher in patients resistant to DNG therapy than in other patients. Thus, azurocidin may be associated with DNG resistance in OMAs.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"702-715"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Branched-chain and Aromatic Amino Acids in Blood and Seminal Plasma are Associated with Sperm Parameters; A Practice within a Fertility Clinic Considering the Metabolic Syndrome. 血液和精浆中支链氨基酸和芳香氨基酸与精子参数的关系考虑代谢综合征的生育诊所的实践。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI: 10.1007/s43032-024-01779-2

Vahid Esmaeili, AliReza Alizadeh, Morteza Zendehdel, Masoud Habibi, Adel Pezeshki, Alireza Dizavi, Samira Vesali, Mohammad Ali Sadighi Gilani, Mehrzad Nahid, Abdolhossein Shahverdi

{"title":"Branched-chain and Aromatic Amino Acids in Blood and Seminal Plasma are Associated with Sperm Parameters; A Practice within a Fertility Clinic Considering the Metabolic Syndrome.","authors":"Vahid Esmaeili, AliReza Alizadeh, Morteza Zendehdel, Masoud Habibi, Adel Pezeshki, Alireza Dizavi, Samira Vesali, Mohammad Ali Sadighi Gilani, Mehrzad Nahid, Abdolhossein Shahverdi","doi":"10.1007/s43032-024-01779-2","DOIUrl":"10.1007/s43032-024-01779-2","url":null,"abstract":"The metabolomic approach has recently been used in the assessment of semen quality and male fertility. Additionally, the crucial roles of branched-chain amino acids (BCAAs) and aromatic amino acids (AAAs) in metabolic syndrome (MetS) were reported. However, little information exists about the association between BCAAs and AAAs with semen parameters, particularly in men with and without MetS. Our objective was to explore the association between BCAAs and AAAs in blood and seminal plasma and sperm parameters in men with MetS (MetS +) and without MetS (MetS-). In a cross-sectional study between January-July 2022, we investigated 98 men (age: 25-42 years; MetS + : n = 28 and MetS-: n = 70) at Royan Institute, Tehran, Iran. All participants underwent anthropometric indices measurements using standard protocols. From each participant, a single fasting blood sample was collected on the same day that the semen sample was collected. The BCAAs and AAAs in blood and seminal plasma were measured using high-performance liquid chromatography (HPLC). The aromatase activity (total testosterone/ estradiol ratio) was significantly lower in MetS + (0.16) than MetS- (0.35) (p = 0.016). Semen parameters were similar between the MetS + and the MetS- groups. All BCAA and AAA family members, except tryptophan, were higher in the blood plasma of men with metabolic syndrome. Meanwhile, the seminal plasma of BCAA and AAAs were similar. Intriguingly, Valine in blood (r = -0.329; p < 0.001) and seminal (r = -0.237; p < 0.05) plasma were correlated with abnormal sperm morphology in patients without metabolic syndrome (MetS-). Further research is necessary to validate these findings and to explore the underlying mechanisms and interactions between the plasma BCAAs and AAAs and sperm parameters.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"757-768"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonography Findings in Ovarian Hemangiomas: Report of Three Cases and Review of the Literature. 卵巢血管瘤的超声波检查结果：三例病例报告及文献综述。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2024-07-17 DOI: 10.1007/s43032-024-01656-y

Yue Qi, Lianting Zhuang, Tiantong Zhu

引用次数: 0

Piceatannol Protects Sperm from Cryopreservation Damage by Modulating the Keap1-Nrf2/ARE Signaling Pathway. 皮夏单酚通过调节 Keap1-Nrf2/ARE 信号通路保护精子免受冷冻损伤

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2024-10-24 DOI: 10.1007/s43032-024-01723-4

Lijie Fu, Chao Wang, Wenfu Li, Hao Dong, Qian Yang, Guilin Chang, Jianping Liu

{"title":"Piceatannol Protects Sperm from Cryopreservation Damage by Modulating the Keap1-Nrf2/ARE Signaling Pathway.","authors":"Lijie Fu, Chao Wang, Wenfu Li, Hao Dong, Qian Yang, Guilin Chang, Jianping Liu","doi":"10.1007/s43032-024-01723-4","DOIUrl":"10.1007/s43032-024-01723-4","url":null,"abstract":"The purpose of this study was to explore the mechanism of action of Piceatannol (PIC) in attenuating oxidative damage to sperm during cryopreservation. Semen samples were collected and homogenized into six equal parts for freeze-thawing experiments. Four different concentrations of PIC were utilized as cryoprotectants during the freeze-thawing process, maintaing a semen to PIC ratio of 1:1, while sperm motility after freezing and thawing was analyzed using computer-assisted sperm analysis (CASA). Sperm plasma membrane integrity was assessed via the hypo-osmotic swelling (HOS) test. The levels of reactive oxygen species (ROS), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) activities, long with the ability to scavenge sperm malondialdehyde (MDA), were examined in sperm following the addition of PIC. Quantitative real-time PCR (qRT-PCR) was performed to detect the expression levels of Keap1, Nrf2, GCLC, GCLM, and HMOX1 in sperm. The mechanism by which PIC protects sperm during cryopreservation from oxidative stress damage was further verified. Treatment with PIC at a dose of 5.0 μmol/L significantly improved both sperm motility and viability while effectively reducing ROS levels in frozen sperm. Additionally, the integrity of the sperm plasma membrane was significantly enhanced. Furthermore, the expression level of Keap1 was significantly reduced, whereas the expression levels of GCLC, GCLM, HMOX1, and Nrf2 were significantly increased (p < 0.05) after the addition of PIC. Notably, a significant attenuation of sperm motility and viability was observed in this treatment group when PIC treatment was accompanied by the addition of an Nrf2 inhibitor, resulting in a significant elevation of ROS levels. The finding that PIC modulates ROS in frozen sperm via the Keap1-Nrf2/ARE pathway thereby enhancing sperm viability levels after freezing and thawing provides a novel approach to optimize semen cryopreservation.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"783-791"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycystic Ovarian Syndrome: A Review of Multi-omics Analyses. 多囊卵巢综合征：多组学分析综述。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-28 DOI: 10.1007/s43032-025-01789-8

Ilmas Naqvi, Anannya Bandyopadhyay, Amisha Panda, B Hareramadas

{"title":"Polycystic Ovarian Syndrome: A Review of Multi-omics Analyses.","authors":"Ilmas Naqvi, Anannya Bandyopadhyay, Amisha Panda, B Hareramadas","doi":"10.1007/s43032-025-01789-8","DOIUrl":"10.1007/s43032-025-01789-8","url":null,"abstract":"Polycystic Ovary Syndrome (PCOS) is among the most prevalent endocrinological abnormalities of young females, posing a grave public health challenge to the society. The objective of the present literature review is to analyze the enormous amount of information available by way of numerous multi-omic studies, and to explore a meaningful relationship between various factors such as genetic, proteomic, environmental etc. to understand the multifactorial metabolic disorder in a proper manner. Detailed literature search was done in various science article repositories and biomedical databases such as PubMed, Google Scholar, BioMed Central, Embase etc. by using several keywords in whole gamut of combinations. PCOS is a heritable disease. It manifests as a result of a combination of several intricately inter-linked symptoms such as anovulation, obesity, type II diabetes, hyperandrogenism, polycystic ovaries etc., the last one being the main manifestation of the disease, thus leading to infertility among several other complications. Such a multifactorial metabolic disorder with extreme symptomatic heterogeneity cannot be fully explained solely based on symptoms or genetic variations; thus, giving some space of thought to other factors such as epigenetic, microbiomic factors etc. playing a role in the causation of the disease. The present scientific survey of literature extensively reviews various aspects of PCOS by critically looking into the vast multi-omic data, and concluded with suggesting treatment options as well as lifestyle changes required to deal with the psychological/ emotional impacts of the condition on affected women.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"618-646"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenesis, Diagnosis, and Management of Inguinal Endometriosis: A Case Series of Six Patients. 腹股沟子宫内膜异位症的发病、诊断和治疗：6例患者的病例系列。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-24 DOI: 10.1007/s43032-025-01796-9

Saki Takatsuka, Hisashi Kataoka, Fumitake Ito, Koki Shimura, Taisuke Mori

引用次数: 0

The Impact of Inflammatory Cytokines on Recurrent Pregnancy Loss: A Preliminary Investigation. 炎性细胞因子对复发性妊娠丢失的影响：初步研究。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-22 DOI: 10.1007/s43032-025-01786-x

Shivani Mishra, Ashish Ashish, Sangeeta Rai, Chetan Sahni, Shivam Tiwari, Bhupendra Kumar, Royana Singh

{"title":"The Impact of Inflammatory Cytokines on Recurrent Pregnancy Loss: A Preliminary Investigation.","authors":"Shivani Mishra, Ashish Ashish, Sangeeta Rai, Chetan Sahni, Shivam Tiwari, Bhupendra Kumar, Royana Singh","doi":"10.1007/s43032-025-01786-x","DOIUrl":"10.1007/s43032-025-01786-x","url":null,"abstract":"Recurrent pregnancy loss (RPL), defined as two or more consecutive miscarriages before 20 weeks of gestation, affects 1-2% of couples worldwide. Pro-inflammatory cytokines, such as TNF-α, IL-1β and IL-6 play critical roles in early pregnancy, while anti-inflammatory cytokines like TGF-β and IL-10 promote immune tolerance to prevent harmful inflammatory responses that play important role in placental and fetal development. This aim of the study is to analyse the levels of inflammatory cytokines in blood serum from RPL patients and healthy women (control). The measured cytokines included TNF-α, IL-6, IL-10, TGF-β, CRP, ferritin, IL-1β and IL-4, IFN-γ and IL-17. Using an unpaired t-test and Pearson correlation, significant difference observed between the groups. The results had significantly elevated CRP levels with decreased levels of TGF-β and ferritin (p < 0.05), whereas, IL-1β and IL-4 also found raised indicating a link between systemic inflammation and recurrent miscarriages. IL-4 and CRP increase further suggest potential oxidative stress role in RPL cases. However, no significant differences observed in IL-10, IL-6, or TNF-α level between the groups. This study highlights immune dysregulation as possible contributors to early pregnancy loss, with significant increases in CRP, IL-1β, and IL-4 levels indicating an imbalanced immune response at the maternal-fetal interface. These cytokine elevations may disrupt immune tolerance, suggesting the need for further exploration into cytokine interactions in pregnancy and their potential as an investigatory biomarker and therapeutic target in RPL.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"804-814"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Effect of Combined Placenta-Derived Mesenchymal Stem Cells plus Platelet-rich Plasma on Polycystic Ovarian Model in Rats: A Biochemical and Histological Study. 胎盘源间充质干细胞联合富血小板血浆对大鼠多囊卵巢模型的改善作用：生化和组织学研究。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-03-01 Epub Date: 2025-01-24 DOI: 10.1007/s43032-025-01791-0

Mojtaba Sarvestani, Alireza Rajabzadeh, Morteza Salimian, Tahereh Mazoochi, Gholamreza Ghavipanjeh

{"title":"Ameliorative Effect of Combined Placenta-Derived Mesenchymal Stem Cells plus Platelet-rich Plasma on Polycystic Ovarian Model in Rats: A Biochemical and Histological Study.","authors":"Mojtaba Sarvestani, Alireza Rajabzadeh, Morteza Salimian, Tahereh Mazoochi, Gholamreza Ghavipanjeh","doi":"10.1007/s43032-025-01791-0","DOIUrl":"10.1007/s43032-025-01791-0","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a common cause of infertility in women, characterized by metabolic and hormonal irregularities. We investigated the effects of placenta-derived mesenchymal stem cells (PDMSCs) and platelet-rich plasma (PRP), as well as their combination on follicular development, hormonal profile, inflammatory parameters, and insulin resistance in a model of PCOS. In this study, 25 female Wistar rats were randomly allocated into five groups: Sham (given a dose of 1 mL of a 0.5% carboxymethylcellulose (CMC) solution), PCOS (administered 1 mg/kg of letrozole (LTZ) dissolved in CMC for 21 days), PDMSC (treated with a single intraovarian dose of PDMSCs), PRP (treated with a single intraovarian dose of PRP), and a combined PDMSC and PRP-treated group. After two weeks, serum and ovarian samples were collected for biochemical and histological analyses. Our results demonstrated that the simultaneous administration of PDMSCs and PRP had a synergistic effect compared to monotherapy, leading to an increase in estradiol (E2) and follicle-stimulating hormone (FSH) serum levels, a decrease in luteinizing hormone (LH) and testosterone levels, as well as inflammatory factors. Moreover, the combined therapy was associated with significantly lower levels of the homeostatic model of insulin resistance (HOMA-IR), fasting insulin (FINS), and blood glucose (FBG) compared to monotherapy. The combined treatment also caused a significant reduction in cystic follicles and an elevated number of corpus luteum, primordial, primary, secondary, and antral follicles. In conclusion, the combination of PRP and PDMSCs may have an ameliorative effect on modifying metabolic abnormalities and accelerating ovarian regeneration in PCOS.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"907-918"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0