Reproductive Sciences最新文献_第8页

Reclassifying a Novel POMT1 Variant by Integrating Functional Analysis and Bioinformatics: Implications for Preimplantation Genetic Testing. 通过整合功能分析和生物信息学重新分类新的POMT1变异：植入前基因检测的意义。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2024-12-30 DOI: 10.1007/s43032-024-01761-y

Binyu Ma, Zhidan Hong, Li Zhang, Ling Ma, Jie Duan, Ying Gao, Mei Wang, Yuanzhen Zhang

{"title":"Reclassifying a Novel POMT1 Variant by Integrating Functional Analysis and Bioinformatics: Implications for Preimplantation Genetic Testing.","authors":"Binyu Ma, Zhidan Hong, Li Zhang, Ling Ma, Jie Duan, Ying Gao, Mei Wang, Yuanzhen Zhang","doi":"10.1007/s43032-024-01761-y","DOIUrl":"10.1007/s43032-024-01761-y","url":null,"abstract":"The advancement of next-generation sequencing has spurred the growing adoption of whole-exome sequencing (WES) for genetic screening. Preimplantation genetic testing for monogenic disorders (PGT-M) can effectively prevent the transmission of pathogenic variants. However, interpreting vast data volumes and ensuring precise genetic counseling, especially with variants of uncertain significance (VUS), remains challenging. In this study, we investigated a family with recurrent fetal malformations detected by prenatal ultrasound. WES identified compound heterozygous POMT1 variants, c.1052 + 1G > A and c.1483G > A in the proband; the latter was initially categorized as a VUS. Then our bioinformatics analysis revealed that c.1483G > A variant was located in a highly conserved domain essential for POMT1's enzymatic activity, potentially altering the protein's 3D structure. In vitro studies using HEK293T cells showed that the variant led to aberrant POMT1 mRNA and protein accumulation, impaired cell viability, and abnormal protein localization in the cytoplasm, indicating disruption of normal glycosylation processes. Combining bioinformatics analysis with in vitro experiments, we reclassified the c.1483G > A variant as likely pathogenic. Subsequently, the couple opted for PGT-M, culminating in the birth of a healthy child. Our findings underscore the pivotal role of genetic testing in recurrent fetal malformations and expand the spectrum of POMT1 variants. The successful reclassification of the variant by integrating in vitro experiments with bioinformatics provides substantial evidence for clinicians implementing PGT-M, offering a feasible strategy for counseling with VUS detected by WES.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1612-1625"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of BMI on PCOS Patients and Transcriptome Profiling and Bioinformatic Analysis of Granulosa Cells in PCOS Patients with High and Low BMI. BMI对多囊卵巢综合征患者的影响及高、低BMI多囊卵巢综合征患者颗粒细胞转录组和生物信息学分析

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2025-01-16 DOI: 10.1007/s43032-024-01783-6

Ping Tao, Xiaohong Yan, Youzhu Li, Zhanxiang Wang

{"title":"The Impact of BMI on PCOS Patients and Transcriptome Profiling and Bioinformatic Analysis of Granulosa Cells in PCOS Patients with High and Low BMI.","authors":"Ping Tao, Xiaohong Yan, Youzhu Li, Zhanxiang Wang","doi":"10.1007/s43032-024-01783-6","DOIUrl":"10.1007/s43032-024-01783-6","url":null,"abstract":"Purpose: To explore the impact of high body mass index (BMI) on the embryo quality and clinical outcomes of polycystic ovary syndrome (PCOS) patients, and the possible genes involved.Methods: Patients who underwent in-vitro fertilization (IVF) treatment and embryo transfer in our center from November 2014 to September 2023, were divided into low BMI PCOS (LBP) group, high BMI PCOS (HBP) group, and high BMI control (HBC) group. Transcriptome sequencing was performed in eight PCOS patients' granulosa cells (GCs).Results: A total of 812 IVF/intracytoplasmic sperm injection (ICSI) cycles in the embryo part; and 489 fresh, 634 frozen-warmed embryo transfer (FET) cycles from the clinical part were included. The ICSI normal fertilization rate of HBP group was decreased compared to LBP and HBC groups (p = 0.013&0.008). The IVF blastocyst development rate in HBP group was lower than LBP group (p = 0.01). The preterm birth rate in HBP group was higher than in LBP (30.66% vs. 16.48%, p = 0.041) and HBC groups (30.66% vs. 11.34%, p = 0.002), the adjusted OR (AOR) of preterm birth and BMI was 1.124 (p = 0.023) in FET cycles. Transcriptome sequencing result of GCs showed that differentially expressed miRNAs/lncRNA/circRNA/mRNAs in two PCOS groups were 61, 450, 83, and 568, respectively. The hub genes analysis, enrichment analysis and competing endogenous RNA network revealed that cell cycle, oocyte maturation, systemic lupus erythematosus, oxidative phosphorylation, and mitogen-activated protein kinases (MAPK) signaling pathways had important roles in the embryo development and pregnancy process.Conclusions: The combined effect of PCOS and obesity reduced oocyte quality and embryonic development potential, finally led to poorer clinical outcomes.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1626-1643"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning for Predicting Stillbirth: A Systematic Review. 预测死产的机器学习：系统回顾

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2024-07-29 DOI: 10.1007/s43032-024-01655-z

Qingyuan Li, Pan Li, Junyu Chen, Ruyu Ren, Ni Ren, Yinyin Xia

{"title":"Machine Learning for Predicting Stillbirth: A Systematic Review.","authors":"Qingyuan Li, Pan Li, Junyu Chen, Ruyu Ren, Ni Ren, Yinyin Xia","doi":"10.1007/s43032-024-01655-z","DOIUrl":"10.1007/s43032-024-01655-z","url":null,"abstract":"Stillbirth is a major global issue, with over 5 million cases each year. The multifactorial nature of stillbirth makes it difficult to predict. Artificial intelligence (AI) and machine learning (ML) have the potential to enhance clinical decision-making and enable precise assessments. This study reviewed the literature on predictive ML models for stillbirth highlighting input characteristics, performance metrics, and validation. The PubMed, Cochrane, and Web of Science databases were searched for studies using AI to develop predictive models for stillbirth. Findings were analyzed qualitatively using narrative synthesis and graphics. Risk of bias and the applicability of the studies were assessed using PROBAST. Model design and performance were discussed. Eight studies involving 14,840,654 women with gestational ages ranging from 20 weeks to full term were included in the qualitative analysis. Most studies used neural networks, random forests, and logistic regression algorithms. The number of predictive features varied from 14 to 53. Only 50% of studies validated the models. Cross-validation was commonly employed, and only 25% of studies performed external validation. All studies reported area under the curve as a performance metric (range 0.54-0.9), and five studies reported sensitivity (range, 60- 90%) and specificity (range, 64 - 93.3%). A stacked ensemble model that analyzed 53 features performed better than other models (AUC = 0.9; sensitivity and specificity > 85%). Available ML models can attain a considerable degree of accuracy for prediction of stillbirth; however, these models require further development before they can be applied in a clinical setting.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1388-1398"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Blastocyst Morphology and Developmental Rate on Euploidy and Live Birth Rates in PGT-A Cycles: A Retrospective Cohort Study. PGT-A周期中囊胚形态和发育率对整倍体和活产率的影响：一项回顾性队列研究。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2025-02-21 DOI: 10.1007/s43032-025-01818-6

Xiaojiao Chen, Yong Fan, Hui Ji, Lin Zhou, Xun Wu, Yi Wei, Shanren Cao, Junqiang Zhang, Xiufeng Ling

{"title":"Effect of Blastocyst Morphology and Developmental Rate on Euploidy and Live Birth Rates in PGT-A Cycles: A Retrospective Cohort Study.","authors":"Xiaojiao Chen, Yong Fan, Hui Ji, Lin Zhou, Xun Wu, Yi Wei, Shanren Cao, Junqiang Zhang, Xiufeng Ling","doi":"10.1007/s43032-025-01818-6","DOIUrl":"10.1007/s43032-025-01818-6","url":null,"abstract":"This study was to evaluate the relationship between morphology or developmental rate of blastocysts and ploidy status in preimplantation genetic testing for aneuploidy (PGT-A) cycles, or live birth rates in single euploid frozen-thawed embryo transfer (FET) cycles. We focused on infertile patients who underwent PGT-A procedures, following with single euploid FET cycles in the assisted reproduction center from January 2016 to December 2022. Blastocysts were categorized for biopsy, and euploid embryos would be selected for transfer. Multivariate logistic regression was used to assess the effects of blastocoel expansion degree, inner cell mass (ICM) and trophectoderm (TE) grades and developmental stage (Day 5, 6 and 7) on euploidy and live birth rates. A total of 4172 blastocysts from 941 PGT-A cycles were included. Expansion 4 were associated with lower euploidy rate than expansion 5 (P = 0.011) and 6 (P = 0.001). Better ICM (P < 0.05 for A compared to B grade) increased blastocyst euploidy. Euploidy rate was significantly associated with A grade TE (P < 0.001). However, no relationship existed between blastocyst euploidy and developmental rate. Furthermore, live birth rates had no significant effect on most of morphological parameters in euploidy blastocyst FET cycles, only A grade TE was shown higher live birth rate than C grade (P = 0.024). The rate of euploidy in morphologically poor blastocysts is low in the cohort, but the developmental rate does not associate with euploidy. Moreover, only TE grades take association with live birth rates, when the single euploidy blastocyst was transplanted.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1705-1712"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Retrospective Analysis of Meiotic Segregation Pattern and Reproductive Outcomes in Blastocysts from Robertsonian Preimplantation Genetic Testing Cycles. 更正：来自罗伯逊着床前基因检测周期的囊胚减数分裂分离模式和生殖结果的回顾性分析。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 DOI: 10.1007/s43032-025-01855-1

Miaomiao Jia, Juanzi Shi, Xia Xue

引用次数: 0

Comparison of GLP-1 Receptor Agonists Combined with Metformin Versus Metformin Alone in the Management of PCOS: A Comprehensive Meta-Analysis. GLP-1受体激动剂联合二甲双胍与单用二甲双胍治疗PCOS的比较：一项综合荟萃分析。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2025-01-29 DOI: 10.1007/s43032-025-01788-9

Yuzi Zhao, Li Jiang, Na Li, Jing Cao, Jie Pi

{"title":"Comparison of GLP-1 Receptor Agonists Combined with Metformin Versus Metformin Alone in the Management of PCOS: A Comprehensive Meta-Analysis.","authors":"Yuzi Zhao, Li Jiang, Na Li, Jing Cao, Jie Pi","doi":"10.1007/s43032-025-01788-9","DOIUrl":"10.1007/s43032-025-01788-9","url":null,"abstract":"This study compared the efficacy and safety of glucogan-like peptide-1 receptor agonists (GLP1RAs) combined with metformin versus metformin alone in women with polycystic ovary syndrome (PCOS). A systematic search of \"PubMed\", \"EMBASE\", \"Cochrane Library\", and \"Web of Science\", \"Google Scholar\" was conducted up to September 2024. Studies were included if they were RCTs investigating the combination of GLP1RAs and metformin in women diagnosed with PCOS. Eligible studies compared this combination therapy to metformin alone. Primary outcomes included changes in body weight, BMI, waist circumference, fasting glucose, HOMA-IR, androgen levels, and sex hormone-binding globulin (SHBG). Meta-analysis was performed using RevMan 5.4 software. Eight RCTs with a total of 337 participants were included. The combination of GLP1RAs and metformin resulted in significant reductions in body weight (mean difference [MD]=-1.37 kg, 95% CI [-2.07, -0.67]; P = 0.0001), BMI (MD= -0.88 kg/m², 95% CI [-1.37, -0.39]; P = 0.0005), waist circumference (MD= -2.46 cm, 95% CI [-3.59,-1.33]; P < 0.0001), fasting glucose level (MD= -0.30, 95% CI [-0.42,-0.17]; P < 0.0001), and glucose level at 2 h after OGTT (MD= -1.58, 95% CI [-2.10,-1.06]; P < 0.0001) compared to metformin alone. Improvements in insulin sensitivity (HOMA-IR) in GLP1RA + Met group were also observed (MD=-1.58, 95% CI [-2.10, -1.06]; P < 0.0001) comparing to Met group. Hormonal outcomes demonstrated an increase in SHBG (MD = 10.04, 95% CI [7.06, 13.01]; P < 0.0001). Adverse events were not different between GLP1RA + Met and Met groups. Collectivley, combination of GLP1RAs and metformin provides superior benefits over metformin alone in reducing body weight, improving insulin sensitivity, and regulating hormonal imbalances in women with PCOS.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1661-1675"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Expression of Programmed Cell Death Ligand-1 and its Relationship with Infiltration, Metastasis and Prognosis in Cervical Squamous Cell Carcinoma. 细胞程序性死亡配体-1在宫颈鳞癌中的表达及其与浸润、转移和预后的关系

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2025-01-30 DOI: 10.1007/s43032-024-01784-5

Juyan Ge, Xiujuan Xu

{"title":"The Expression of Programmed Cell Death Ligand-1 and its Relationship with Infiltration, Metastasis and Prognosis in Cervical Squamous Cell Carcinoma.","authors":"Juyan Ge, Xiujuan Xu","doi":"10.1007/s43032-024-01784-5","DOIUrl":"10.1007/s43032-024-01784-5","url":null,"abstract":"Cervical cancer (CC) represents a major gynecologic health problem. Respecting the role of programmed cell death ligand-1 (PDL-1) in cancer prognosis, we investigated its relationship with cervical squamous cell carcinoma (CSCC) invasion, metastasis and prognosis. A total of 184 CSCC patients were retrospectively selected, with normal paracarcinoma tissues as the Control group. PDL-1 expression was assessed, and its relationship with CSCC prognosis and clinical value on predicting CSCC invasion/metastasis and poor prognosis were determined. PDL-1 was up-regulated in CSCC. CSCC patients at International Federation of Gynecology and Obstetrics stage II/III, and with lymph node metastasis (LNM), parauterine/vascular infiltration, and history of sexually transmitted diseases exhibited up-regulated PDL-1. The areas under the curve of PDL-1 on predicting the invasion and metastasis/poor prognosis of CSCC patients were 0.930 (95%Cl: 0.883-0.962)/0.935 (95%Cl: 0.886-0.967), with cut-off values of 23.27/24.86 (86.76%/80.95% sensitivity, 95.69%/92.68% specificity). The CSCC patients with highly-expressed PDL-1 showed increased cumulative incidence of poor prognosis. Additionally, occurence of vascular infiltration/LNM, and up-regulated PDL-1 were independent risk factors for poor prognosis in CSCC patients. Briefly, PDL-1 expression rised in CSCC. High PDL-1 expression might promote tumor infiltration and LNM, while close monitoring of its expression contributed to evaluating prognosis of CSCC patients.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1676-1684"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Thyroid Hormones on Cellular Development in Human Ovarian Granulosa Tumor Cells (KGN). 甲状腺激素对人类卵巢颗粒细胞（KGN）细胞发育的影响

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2024-10-15 DOI: 10.1007/s43032-024-01721-6

Yakun Yu, Yilin Yao, Yan Liu, Ying Sun, Haoyuan Feng, Nana Kong, Rui Chen, Mingqi Wu, Shuaitian Guo, Shen Tian, Cheng Zhang

{"title":"Effects of Thyroid Hormones on Cellular Development in Human Ovarian Granulosa Tumor Cells (KGN).","authors":"Yakun Yu, Yilin Yao, Yan Liu, Ying Sun, Haoyuan Feng, Nana Kong, Rui Chen, Mingqi Wu, Shuaitian Guo, Shen Tian, Cheng Zhang","doi":"10.1007/s43032-024-01721-6","DOIUrl":"10.1007/s43032-024-01721-6","url":null,"abstract":"Ovarian cancer is a common malignant tumor in the female reproductive system, and Granulosa cell tumor (GCT) of the ovary is a rare type of ovarian cancer, which significantly threatens women's reproductive health. It has been reported that dysregulation of thyroid hormones (THs) may be closely related to the progression and prognosis of ovarian cancer. Moreover, THs regulate phosphorylation of signal transducer and activator of transcription (STAT3) and Octamer-binding transcription factor 4 (OCT4) expression. It has been reported that STAT3 and OCT4 play important roles in cellular development and tumorigenesis. However, the mechanisms by which THs affect the development of GCT are still remained unclear. To evaluate the effect of THs on human ovarian granulosa tumor cells (KGN), cells were treated with 3,5,3' -triiodothyronine (T3). Oct4 small interfering (Oct4 siRNA) or STAT3 inhibitor C188-9 was also co-cultured with cells in some experiments, respectively. The cell viability, proliferation, and proteins content were detected by CCK-8, EdU, and Western Blotting, respectively. The results showed that T3 enhanced cell viability and proliferation. Moreover, T3 also increased the expression of thyroid hormone receptor (TR), p-STAT3, and OCT4 proteins. The effects of T3 on both p-STAT3 and OCT4 expression were blocked by TR antagonist 1-850. Meanwhile, C188-9, an inhibitor of STAT3, decreased T3-induced cellular viability, proliferation, and OCT4 expression, highlighting that p-STAT3 can regulate the expression of OCT4 and affect cellular viability, and proliferation. Furthermore, T3-induced cellular growth was reduced by Oct4 siRNA, which indicates that T3 regulates cellular development through OCT4. These findings suggest that T3 increases cellular development via OCT4, which is mediated by phosphorylation of STAT3, and TR is also involved in these processes.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1545-1556"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Quality Control in Ovarian Function: From Mechanisms to Therapeutic Strategies. 卵巢功能中的线粒体质量控制：从机制到治疗策略

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2024-07-09 DOI: 10.1007/s43032-024-01634-4

Xiaomei Wang, Yuxin Liu, Jinzheng Wang, Xueyi Lu, Zhipeng Guo, Shenmin Lv, Zhenyu Sun, Tan Gao, Fei Gao, Jinxiang Yuan

{"title":"Mitochondrial Quality Control in Ovarian Function: From Mechanisms to Therapeutic Strategies.","authors":"Xiaomei Wang, Yuxin Liu, Jinzheng Wang, Xueyi Lu, Zhipeng Guo, Shenmin Lv, Zhenyu Sun, Tan Gao, Fei Gao, Jinxiang Yuan","doi":"10.1007/s43032-024-01634-4","DOIUrl":"10.1007/s43032-024-01634-4","url":null,"abstract":"Mitochondrial quality control plays a critical role in cytogenetic development by regulating various cell-death pathways and modulating the release of reactive oxygen species (ROS). Dysregulated mitochondrial quality control can lead to a broad spectrum of diseases, including reproductive disorders, particularly female infertility. Ovarian insufficiency is a significant contributor to female infertility, given its high prevalence, complex pathogenesis, and profound impact on women's health. Understanding the pathogenesis of ovarian insufficiency and devising treatment strategies based on this understanding are crucial. Oocytes and granulosa cells (GCs) are the primary ovarian cell types, with GCs regulated by oocytes, fulfilling their specific energy requirements prior to ovulation. Dysregulation of mitochondrial quality control through gene knockout or external stimuli can precipitate apoptosis, inflammatory responses, or ferroptosis in both oocytes and GCs, exacerbating ovarian insufficiency. This review aimed to delineate the regulatory mechanisms of mitochondrial quality control in GCs and oocytes during ovarian development. This study highlights the adverse consequences of dysregulated mitochondrial quality control on GCs and oocyte development and proposes therapeutic interventions for ovarian insufficiency based on mitochondrial quality control. These insights provide a foundation for future clinical approaches for treating ovarian insufficiency.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1399-1413"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential Transfer of Blastocysts on Day 5 and Day 6 in Patients with Repeated Implantation Failure in Frozen Embryo Transfer Cycle. 在冷冻胚胎移植周期中反复移植失败患者的第 5 天和第 6 天连续移植囊胚。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2025-05-01 Epub Date: 2024-11-25 DOI: 10.1007/s43032-024-01734-1

Qi Jin, Xiaohua Jiang, Lu Zong, Tong Wu, Shengxia Zheng, Limin Wu, Bo Xu

{"title":"Sequential Transfer of Blastocysts on Day 5 and Day 6 in Patients with Repeated Implantation Failure in Frozen Embryo Transfer Cycle.","authors":"Qi Jin, Xiaohua Jiang, Lu Zong, Tong Wu, Shengxia Zheng, Limin Wu, Bo Xu","doi":"10.1007/s43032-024-01734-1","DOIUrl":"10.1007/s43032-024-01734-1","url":null,"abstract":"The aim of this study was to evaluate which frozen embryo transfer (FET) strategy benefits the recurrent implantation failure (RIF) population. A retrospective study of 336 women with RIF was performed from July 2020 to October 2023. The patients were divided into three groups according to the FET protocol. The three FET protocols included transferring a blastocyst on both days 5 and 6 (Group 1, n = 138); transferring a cleavage-stage embryo on day 3 and a blastocyst on day 5 (Group 2, n = 55); and transferring double blastocysts on day 5 (Group 3, n = 143).The sequential day 5 and day 6 blastocyst ET strategy significantly improved in vitro fertilization success rates compared with those of both the traditional double-blastocyst day 5 transfer strategy and the strategy involving one cleavage-stage embryo transfer on day 3 and one blastocyst transfer on day 5 in RIF patients. Compared with those observed in Day 5 double-blastocyst transfer, the rates of clinical pregnancy and implantation increased (51.75% vs. 64.49%, P < 0.001 and 37.06% vs. 41.3%, P = 0.005, respectively), while the rates of biochemical pregnancy and multiple gestation decreased (18.18% vs. 12.32%, P = 0.006 and 43.24% vs. 28.09%, P = 0.044, respectively) with the new protocol.Blastocysts from sequential embryo transfer on day 5 and day 6 were more effective than those from the traditional FET protocol at improving pregnancy outcomes and lowering the multiple pregnancy rate in RIF patients. When only one or zero good-quality blastocysts are available, sequential blastocyst ET on days 5 and 6 should be considered.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1580-1588"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0