Reproductive Sciences最新文献_第4页

Prognostic Nutritional Index and the Survival of Patients with Endometrial cancer: A Meta-analysis. 预后营养指数与子宫内膜癌患者的存活率：一项 Meta 分析。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1007/s43032-024-01686-6

Li Zhang, Fengliang Wang, Cong Wan, Jichun Tang, Jiarui Qin

{"title":"Prognostic Nutritional Index and the Survival of Patients with Endometrial cancer: A Meta-analysis.","authors":"Li Zhang, Fengliang Wang, Cong Wan, Jichun Tang, Jiarui Qin","doi":"10.1007/s43032-024-01686-6","DOIUrl":"10.1007/s43032-024-01686-6","url":null,"abstract":"The prognostic nutritional index (PNI) has emerged as a potential predictor of clinical outcomes in various cancers. However, a quantativetily analysis of its role in endometrial cancer (EC) remains lacking. This meta-analysis aims to evaluate the prognostic value of PNI on the survival outcomes of patients with EC. A comprehensive literature search was conducted in PubMed, EMBASE, Web of Science, Wanfang, and CNKI to identify relevant cohort studies. Studies were included if they provided sufficient data to calculate hazard ratios (HRs) for overall survival (OS) and progression-free survival (PFS) based on PNI levels. Data extraction and quality assessment were performed independently by two reviewers. Pooled HRs with 95% confidence intervals (CIs) were calculated using a random-effects model to account for heterogeneity. A total of 10 studies, encompassing 3656 patients, met the inclusion criteria. The meta-analysis revealed that a low PNI was significantly associated with poorer OS (HR = 2.01, 95% CI = 1.62-2.49, p < 0.05; I2 = 54%) and PFS (HR = 2.75, 95% CI = 1.74-4.33, p < 0.05; I2 = 78%) in patients with EC. Subgroup analyses indicated that the prognostic impact of PNI was consistent in studies from Asian and non-Asian countries, and across studies with different ages of the patients, cutoff values of PNI, and follow-up duration (p for subgroup difference all > 0.05). In conclusion, the PNI is a prognostic marker for survival in patients with EC.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"3779-3794"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Association Between Maternal ABO Blood Group and the Occurrence of Spontaneous Preterm Birth: A Retrospective Population-Based Cohort Study. 母亲 ABO 血型与自发性早产发生率的关系：一项基于人群的回顾性队列研究。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-09-28 DOI: 10.1007/s43032-024-01705-6

Eyal Rom, Manal Massalha, Offer Erez, Raed Salim

{"title":"The Association Between Maternal ABO Blood Group and the Occurrence of Spontaneous Preterm Birth: A Retrospective Population-Based Cohort Study.","authors":"Eyal Rom, Manal Massalha, Offer Erez, Raed Salim","doi":"10.1007/s43032-024-01705-6","DOIUrl":"10.1007/s43032-024-01705-6","url":null,"abstract":"There is limited and inconsistent evidence that imply a relationship between ABO blood types and rate of preterm birth (PTB). We aim to examine the association between maternal ABO blood group and PTB rate. A retrospective-study conducted at a university teaching institution on data collected between 2013 and 2019. Women who delivered a viable neonate at ≥ 24 weeks without major malformations were included. Indicated PTBs were excluded. PTB and early PTB were defined as deliveries that occurred < 37 and < 34 weeks respectively. PTB was further divided into 3 subgroups according to etiology: membranes rupture, intact membranes, and placental abruption regardless of membranes' status. The primary outcome was spontaneous PTB rate. Of 19,301 women included, PTB and early PTB rates were 7.3% (1,418/19,301) and 2.3% (440/19,301) respectively. Rates of PTB in blood groups A, B, O, and AB, were 7.3%, 6.9%, 7.5%, and 7.5% respectively (p = 0.68). There was no significant difference according to etiology. Rates of early PTB were also comparable (p = 0.63). After adjustment for demographic and obstetric variables, blood type was associated with increased placental abruption rate among women who had early PTB (p = 0.038). Placental abruption rate was significantly higher in group A (22.5%) compared to group B (14.1%), (adjusted p = 0.04) and group O (14.0%), (adjusted p = 0.01). The rate in group AB was 17.1%, (adjusted p = 0.85). In conclusion, no association was found between a particular blood group and PTB rate. Women with group A, admitted in early PTB, had an increased risk that the underlying etiology was placental abruption.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"3697-3704"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DBP Exposure Affects Oocyte Fertilization Via Extracellular Vesicles-Derived miR-116-5p in Ovarian Granulosa Cells Through Downregulating FOXO3a Expression. DBP暴露通过下调FOXO3a的表达影响卵巢颗粒细胞中通过细胞外囊泡产生的miR-116-5p的卵母细胞受精。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-06-10 DOI: 10.1007/s43032-024-01559-y

Hongmei Liao, Wenqu Tian, Wen Yao, Qingchun Guo, Yi Wang, Juan Li, Danyu Qing, Yufeng Li, Taoran Deng

{"title":"DBP Exposure Affects Oocyte Fertilization Via Extracellular Vesicles-Derived miR-116-5p in Ovarian Granulosa Cells Through Downregulating FOXO3a Expression.","authors":"Hongmei Liao, Wenqu Tian, Wen Yao, Qingchun Guo, Yi Wang, Juan Li, Danyu Qing, Yufeng Li, Taoran Deng","doi":"10.1007/s43032-024-01559-y","DOIUrl":"10.1007/s43032-024-01559-y","url":null,"abstract":"Mono-butyl phthalate (MBP), the metabolite of dibutyl phthalate (DBP), is the most abundant phthalate metabolite found in Chinese women. Extracellular vesicles (EVs) are nanoscale lipid bilayer particles produced by extensive kinds of cells, serving a key role in intercellular communication. Extracellular vesicle miRNAs (EV-miRNAs) in follicular fluid (FF) have been evidenced to be associated with female reproductive health. The objective of this study was to investigate the associations of EV-miRNAs expressed profile with DBP exposure in FF of female participants and expose its potential mechanism in impaired oocyte development. Based on participants' FF MBP concentrations and fertilization status, we compared the miRNA expression between the FF-EVs of group A (high DBP exposure and impaired fertilization) and group B (low DBP exposure and normal fertilization). Compared with group B, miR-1246, miR-3679-5p, miR-423-5p, miR-5585-3p, miR-116-5p, miR-172-5p were upregulated, while miR-34b-3p was downregulated in group A. Target genes of the differently expressed miRNAs were predicted, and the functional analysis was performed. Furthermore, we exposed human ovarian granulosa tumor cell line (KGN) to MBP (4ug/L) to isolate the EVs from the culture medium and validated the expression levels of different miRNAs. We found that MBP exposure was significantly associated with increased levels of miR-116-5p (P = 0.01). In addition, we demonstrated that the most different miRNA, miR-116-5p regulated oocyte fertilization by inhibiting FOXO3a. Our findings suggested that EV-miRNAs in the FF might mediate MBP toxicity in oocytes.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"3858-3869"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Cuproptosis-Related Gene FDX1 Identified as a Potential Target for Human Ovarian Aging. 更正：杯突相关基因 FDX1 被确定为人类卵巢衰老的潜在靶点。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 DOI: 10.1007/s43032-024-01670-0

Chia-Chun Wu, Chia-Jung Li, Li-Te Lin, Pei-Hsuan Lin, Zhi-Hong Wen, Jiin-Tsuey Cheng, Kuan-Hao Tsui

引用次数: 0

Novel Loss-of-function Variants of ZP3 Associated with Premature Ovarian Insufficiency. 与卵巢早衰有关的新型 ZP3 功能缺失变异基因

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-11-01 DOI: 10.1007/s43032-024-01732-3

Lang Zhou, Xi Yang, Shuting Ren, Yuncheng Pan, Zixue Zhou, Yiqing Liu, Jitong Mo, Feng Zhang, Xiaojin Zhang, Yanhua Wu

{"title":"Novel Loss-of-function Variants of ZP3 Associated with Premature Ovarian Insufficiency.","authors":"Lang Zhou, Xi Yang, Shuting Ren, Yuncheng Pan, Zixue Zhou, Yiqing Liu, Jitong Mo, Feng Zhang, Xiaojin Zhang, Yanhua Wu","doi":"10.1007/s43032-024-01732-3","DOIUrl":"10.1007/s43032-024-01732-3","url":null,"abstract":"Premature ovarian insufficiency (POI) is one of the leading causes of female infertility. To date, the genetic etiology of POI has been elucidated in approximately 20-25% of the total cases. The human zona pellucida (ZP) plays an important role in the organization and differentiation of granulosa cells, follicle formation, and sperm recognition. Mutations in ZP1, ZP2, and ZP3 have been reported to cause female infertility due to oocyte degeneration, empty follicle, or in vitro fertilization failure. In this study, we identified three novel missense mutations in ZP3 (NM_001110354.2): c.643G > A (p.Asp215Asn), c.215 C > T (p.Thr72Ile), and c.152T > C (p.Leu51Pro) in three sporadic Han Chinese POI patients through whole-exome sequencing. These variants are absent from population databases and were predicted to be deleterious by multiple in silico tools. Structure prediction analysis showed that the affected amino acid altered the ZP3 protein structure. Western blot further confirmed that these ZP3 variants reduced the expression and secretion of ZP components. In summary, this study reports three novel deleterious variants in ZP3 associated with POI, thereby broadening the mutation spectrum of ZP3 in POI patients.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"3919-3928"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Dietary Interventions on Endometriosis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. 饮食干预对子宫内膜异位症的影响：随机对照试验的系统回顾和元分析》。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-10-02 DOI: 10.1007/s43032-024-01701-w

Jéssica Kleinkauff Meneghetti, Mirela Tschiedel Pedrotti, Isabel Meneghetti Coimbra, João Sabino Lahorgue da Cunha-Filho

{"title":"Effect of Dietary Interventions on Endometriosis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.","authors":"Jéssica Kleinkauff Meneghetti, Mirela Tschiedel Pedrotti, Isabel Meneghetti Coimbra, João Sabino Lahorgue da Cunha-Filho","doi":"10.1007/s43032-024-01701-w","DOIUrl":"10.1007/s43032-024-01701-w","url":null,"abstract":"This study aimed to systematically review existing randomized clinical trials on the effect of dietary interventions on endometriosis. A search was performed on the Cochrane Central Register of Controlled Trials (CENTRAL), Cumulative Index to Nursing and Allied Health Literature (CINAHL), Clinical Trials, EMBASE, PubMed, Lilacs, and Cielo databases. The search terms were used: \"endometriosis\", \"endometrioma\", \"diet\", \"supplements\" and \"nutrition\". Electronic literature searches through databases yielded 866 publications. Two authors performed The study selection independently (and blinded), and disagreements were discussed. Eleven RCTs were included in the systematic review, with 716 women randomized. Most studies reported a positive effect on endometriosis pain scores; however, they were characterized by moderate or high-risk bias. Of these, six RCTs, including 457 women with endometriosis, were included in the meta-analysis. Compared with Placebo, antioxidant use was associated with a reduction in dysmenorrhea (mean difference - 1.95 [CI 95%, -3.78 to -0.13]. Supplementation was not significant for reducing chronic pelvic pain (mean difference - 2.22 [95% CI, -4.99 to 0.55] and dyspareunia (MD - 2.56 [95% CI, - 5. 22 to 0.10]. Both analyses showed a high degree of heterogeneity. Moreover, studies with low risk of bias did not show significant results compared to those on Placebo. The effects of nutrient compounds seem to have the potential to reduce pain in women with endometriosis, mainly to reduce dysmenorrhea. However, the available studies present high heterogeneity and moderate/high risk of bias. More randomized clinical trials are needed to accurately determine dietary interventions' short- and long-term efficacy and safety in managing endometriosis pain.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"3613-3623"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc as a Possible Critical Element to Prevent Harmful Effects of COVID-19 on Testicular Function: a Narrative Review. 锌可能是防止 COVID-19 对睾丸功能产生有害影响的关键元素：叙述性综述。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-07-10 DOI: 10.1007/s43032-024-01638-0

Marouane Chemek, Ammar Kadi, Fatimah Kadhim Ibrahim Al-Mahdawi, Irina Potoroko

引用次数: 0

Experimental Varicocele Impairs DNA Methylation and Demethylation in Germ Cells, TESE, and Epididymal Spermatozoa, Impacting Active DNA Demethylation in Zygotes. 实验性精索静脉曲张会损害生殖细胞、TESE和附睾精子中的DNA甲基化和去甲基化，影响子代中活跃的DNA去甲基化。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-12-01 Epub Date: 2024-08-07 DOI: 10.1007/s43032-024-01651-3

Sana Moshari, Mazdak Razi, Mohammad Hossein Nasr-Esfahani, Marziyeh Tavalaee, Mehdi Hajian

{"title":"Experimental Varicocele Impairs DNA Methylation and Demethylation in Germ Cells, TESE, and Epididymal Spermatozoa, Impacting Active DNA Demethylation in Zygotes.","authors":"Sana Moshari, Mazdak Razi, Mohammad Hossein Nasr-Esfahani, Marziyeh Tavalaee, Mehdi Hajian","doi":"10.1007/s43032-024-01651-3","DOIUrl":"10.1007/s43032-024-01651-3","url":null,"abstract":"Varicocele causes infertility. The current study has investigated the impact of experimental varicocele on DNA methylation, demethylation, and damage in the germ cells, TESE-derived and epididymal spermatozoa. Moreover, the results were compared between epidydimal and TESE-derived spermatozoa. Finally, the varicocele-induced effect on active DNA demethylation (ADD) of male pronucleus and pre-implantation embryo development was assessed. The mature male rats were divided into control, control-sham (undergone simple laparotomy), and experimental varicocele-induced groups (n = 6/each group). The left renal vein semi-ligation was considered to induce varicocele. The expression levels of DNA methyltransferase 1 (DNMT1) and ten-eleven-translocation proteins (TET1, 2, 3), and global DNA methylation in testicular tissue, TESE, and epididymis-derived spermatozoa, and the ADD in zygotes male pronucleus as well as pre-implantation embryo development were assessed. The expression levels of DNMT1 and TET1, 2, 3 in testicles, TESE, and epididymis-derived spermatozoa were decreased in the varicocele group compared to the control and control-sham groups. The TESE-derived spermatozoa exhibited higher DNMT1, higher DNMT1, and TET 1, 2, and no change in TET3 expression compared to epididymis-derived spermatozoa. The varicocele group represented lower DNA methylation in the testicles, TESE-derived and epididymal spermatozoa, higher 5mC+ signal in male pronucleus, and a lower pre-implantation embryo development compared to control and control-sham rats. The TESE-derived spermatozoa exhibited higher 5mC protein expression compared to epididymal spermatozoa. In conclusion, varicocele can negatively impact the DNA methylation/demethylation processes impairing spermatogenesis and leading to fertilization failure, which may ultimately result in a decrease in embryo development by increasing susceptibility to DNA damage.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"3808-3824"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential Transfer of Blastocysts on Day 5 and Day 6 in Patients with Repeated Implantation Failure in Frozen Embryo Transfer Cycle. 在冷冻胚胎移植周期中反复移植失败患者的第 5 天和第 6 天连续移植囊胚。

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-11-25 DOI: 10.1007/s43032-024-01734-1

Qi Jin, Xiaohua Jiang, Lu Zong, Tong Wu, Shengxia Zheng, Limin Wu, Bo Xu

{"title":"Sequential Transfer of Blastocysts on Day 5 and Day 6 in Patients with Repeated Implantation Failure in Frozen Embryo Transfer Cycle.","authors":"Qi Jin, Xiaohua Jiang, Lu Zong, Tong Wu, Shengxia Zheng, Limin Wu, Bo Xu","doi":"10.1007/s43032-024-01734-1","DOIUrl":"https://doi.org/10.1007/s43032-024-01734-1","url":null,"abstract":"The aim of this study was to evaluate which frozen embryo transfer (FET) strategy benefits the recurrent implantation failure (RIF) population. A retrospective study of 336 women with RIF was performed from July 2020 to October 2023. The patients were divided into three groups according to the FET protocol. The three FET protocols included transferring a blastocyst on both days 5 and 6 (Group 1, n = 138); transferring a cleavage-stage embryo on day 3 and a blastocyst on day 5 (Group 2, n = 55); and transferring double blastocysts on day 5 (Group 3, n = 143).The sequential day 5 and day 6 blastocyst ET strategy significantly improved in vitro fertilization success rates compared with those of both the traditional double-blastocyst day 5 transfer strategy and the strategy involving one cleavage-stage embryo transfer on day 3 and one blastocyst transfer on day 5 in RIF patients. Compared with those observed in Day 5 double-blastocyst transfer, the rates of clinical pregnancy and implantation increased (51.75% vs. 64.49%, P < 0.001 and 37.06% vs. 41.3%, P = 0.005, respectively), while the rates of biochemical pregnancy and multiple gestation decreased (18.18% vs. 12.32%, P = 0.006 and 43.24% vs. 28.09%, P = 0.044, respectively) with the new protocol.Blastocysts from sequential embryo transfer on day 5 and day 6 were more effective than those from the traditional FET protocol at improving pregnancy outcomes and lowering the multiple pregnancy rate in RIF patients. When only one or zero good-quality blastocysts are available, sequential blastocyst ET on days 5 and 6 should be considered.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylation Status at DMRs of C14MC and C19MC in Spermatozoa and Chorionic Villi of Individuals Experiencing Recurrent Spontaneous Abortions. 复发性自然流产患者精子和绒毛中 C14MC 和 C19MC 的 DMRs 甲基化状况

IF 2.6 3区医学

Reproductive Sciences Pub Date : 2024-11-22 DOI: 10.1007/s43032-024-01737-y

Mamata Datar, Vandana Bansal, Padmaja Samant, Kumari Nishi, Nafisa H Balasinor

{"title":"Methylation Status at DMRs of C14MC and C19MC in Spermatozoa and Chorionic Villi of Individuals Experiencing Recurrent Spontaneous Abortions.","authors":"Mamata Datar, Vandana Bansal, Padmaja Samant, Kumari Nishi, Nafisa H Balasinor","doi":"10.1007/s43032-024-01737-y","DOIUrl":"https://doi.org/10.1007/s43032-024-01737-y","url":null,"abstract":"Recurrent spontaneous abortions (RSA) is defined as a loss of two or more consecutive clinically recognized pregnancies before the 20th week of gestation. In RSA, several causative maternal factors are known, but still, 50% of the cases remain unexplained. Evidence suggests that paternal factors are also equally important. Epigenetic phenomenon such as genomic imprinting and regulation of gene expression by miRNAs plays an important role in embryonic and placental development. Two large miRNA clusters, C14MC (Chromosome 14 microRNA cluster) and C19MC (Chromosome 19 microRNA cluster) are imprinted and expressed in the placenta during pregnancy and are known to regulate functionally important processes such as the trophoblast proliferation, adhesion, and migration. Hence, we studied the DNA methylation at the Differentially Methylated Regions (DMRs) of these clusters in spermatozoa and chorionic villi by pyrosequencing. In Spermatozoa, few Cytosine followed by Guanosine (CpG) sites at DMRs of C14MC and C19MC showed significant hypermethylation. In Chorionic villi, CpG sites showed significant hypomethylation in the RSA group as compared to control group. Semen parameters like sperm concentration, sperm motility, morphology, and chromatin compaction were comparable in control and RSA groups. The study suggests aberrant DNA methylation in spermatozoa and chorionic villi at DMRs of both miRNA coding clusters to be associated with RSA.","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0