Reproductive Sciences最新文献

{"title":"Effect of THBS1 and P21 Gene Polymorphisms and Their Interaction On Risk of Pre-eclampsia.","authors":"Linbin He, Lijuan Wang, Ziqiang Gong, Ziye Li, Fan Xia, Peiwen Wang, Suyan Guo, Shanyu Lin, Lizhang Chen, Tingting Wang","doi":"10.1007/s43032-025-01892-w","DOIUrl":"https://doi.org/10.1007/s43032-025-01892-w","url":null,"abstract":"<p><p>This study aimed to evaluate the association of THBS1 (Thrombospondin 1) and P21 gene polymorphisms and their interaction with the risk of PE. A case-control study was conducted, involving 175 pregnant women with PE and 350 normotensive pregnant women recruited from two tertiary hospitals. Genotyping of 13 single-nucleotide polymorphisms in the THBS1 and P21 genes was performed using the MassArray platform. Univariate and multivariate logistic regression analyses were performed to evaluate the association between THBS1 and P21 gene polymorphisms and risk of PE. Classification and regression tree (CART) analysis was used to identify potential gene-gene interactions, and logistic regression was applied to assess their impact on PE risk. After adjusting for confounding factors, the THBS1 rs2169830 polymorphism was significantly associated with decreased susceptibility to PE (additive model: aOR = 0.12, p = 0.003), while the P21 rs762624 polymorphism was significantly associated with an increased risk of PE (additive model: aOR = 2.24, p = 0.002). CART and logistic regression analyses identified a significant effect of interaction between polymorphisms in rs762624, rs2292305, and rs1478604 on the susceptibility of PE, with individuals carrying the rs762624CC-rs2292305AA/GG-rs1478604TT/CC genotype combination having the highest risk of PE (aOR = 4.533, p < 0.001). Our findings highlight the importance of THBS1 rs2169830 and P21 rs762624 polymorphisms, along with their interaction, in influencing PE risk. The present study provides a foundation for a more profound understanding of the mechanisms underlying PE.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic Guidelines to Integrate Artificial Intelligence in Obstetrics and Gynecology: Best Practices and Ethical Considerations.","authors":"Pallav Sengupta, Sulagna Dutta, Sovan Bagchi, Prashanth Hegde, Suraj Sebastian, David Cm Taylor, Ralf Henkel","doi":"10.1007/s43032-025-01903-w","DOIUrl":"https://doi.org/10.1007/s43032-025-01903-w","url":null,"abstract":"<p><p>The transformative advancements in artificial intelligence (AI) have significantly impacted medical fields, particularly obstetrics and gynecology (OBGYN). This manuscript presents a comprehensive set of twelve strategic guidelines for the effective integration of AI into OBGYN, emphasizing both best practices and ethical considerations. These guidelines cover essential domains including multidisciplinary collaboration, safeguarding patient safety and privacy, continuous staff training, mitigating algorithmic bias, promoting transparent communication with patients, and fostering a continuous feedback loop between clinicians and AI developers. Additional recommendations highlight the importance of staying updated on AI advancements, defining the role of AI within clinical governance frameworks, ensuring long-term sustainability, collaborating with AI vendors for customized solutions, and evaluating outcomes to inform future practice. These strategies are designed to position AI as an augmentative tool in clinical decision-making, ensuring it enhances rather than replaces human expertise. By upholding collaborative efforts and ethical standards, AI can profoundly improve care quality in OBGYN, fostering safer and more effective healthcare delivery.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phospholipases: Paving the Way for a New Life.","authors":"Rohit Kumar, Preeti Vats, Priti Panchal, Sudarshan Kumar","doi":"10.1007/s43032-025-01900-z","DOIUrl":"https://doi.org/10.1007/s43032-025-01900-z","url":null,"abstract":"<p><p>Phospholipases are a large group of enzymes with ramifications on diverse physiological processes. These enzymes are involved in releasing phospholipids, which are subsequently utilized in the production of prostaglandin and calcium signaling. Calcium signaling is central to the function of phospholipases and calcium oscillations have an impact on the variety of events of embryo development. Additionally, the diverse isoforms of the enzymes can activate gene expression and regulatory factors in a stage-specific manner. Any alterations in the level of these enzymes or the pathways regulating these can have a detrimental effect on the development of the embryo and can also cause early embryonic mortality. In addition to contraction, the enzyme is also critical for the maintenance of quiescence in the uterus during pregnancy. This review discusses the crucial roles of the enzymes, starting from sperm capacitation to the parturition and the putative signaling pathways that are involved in these events. The phospholipases has a significant role in many events in the developmental biology of an animal. Any pathology associated with this enzyme group will have significant consequences on the progression of pregnancy.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Maternal Age on the Repairing Capacity of Oocytes on Paternal DNA Damage.","authors":"Seda Karabulut, Pelin Kutlu, Oya Korkmaz, Lima Oria","doi":"10.1007/s43032-025-01911-w","DOIUrl":"https://doi.org/10.1007/s43032-025-01911-w","url":null,"abstract":"<p><p>Sperm DNA fragmentation is a primary cause of male infertility, affecting ART outcomes and success rates. Sperm has no DNA repair mechanisms because it is transcriptionally quiet. Oocytes' repairing capacity is one of the suggested methods to overcome the situation. However, based on the hypothesis that oocyte age may also have an impact on this talent, we aimed to reveal the role of female age in repairing sperm DNA damage in our study. A total of 540 couples undergoing ICSI were grouped based on the maternal age, and the groups were further divided into two groups based on the severity of DNA damage rates, as the low (< 30% SDF) and high DNA fragmentation (≥ 30% SDF). Laboratory and clinical outcomes were compared between the groups. No significant differences were observed in younger patients (≤ 36 y) and patients between 37-40 years. However, when maternal age was > 40, significantly lower embryo and blastocyst quality, blastocyst development, pregnancy, and implantation rates were observed. Our findings suggest that older oocytes may have a reduced ability to repair sperm DNA damage, as demonstrated by poorer IVF outcomes of couples with higher SDF scores and advanced maternal age (≥ 40).</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Estrogen Signaling in Notch Pathway Activation in Sertoli Cells.","authors":"Sylwia Lustofin, Alicja Kamińska, Barbara Bilińska, Julia Wajda, Anna Hejmej","doi":"10.1007/s43032-025-01901-y","DOIUrl":"https://doi.org/10.1007/s43032-025-01901-y","url":null,"abstract":"<p><p>Sertoli cells constitute a somatic component of seminiferous epithelium in mammalian testis which supports the process of spermatogenesis. These cells are sensitive not only to the male sex hormones androgens, but also to their metabolites, estrogens. In this study, we aimed to explore the action of estrogen signaling in rodent Sertoli cells on the activity of canonical Notch pathway, which provides contact-dependent communication between the cells of seminiferous epithelium. Experiments performed on Sertoli cell line and primary Sertoli cell cultures demonstrated that 17β-estradiol, acting through estrogen receptor 2, enhances Notch1 activation, transcriptional activity of recombination signal binding protein for immunoglobulin kappa J region (RBPJ), interaction of RBPJ with Hes1 promoter, and the expression of the Hes1 effector gene and protein. On the other hand, 17β-estradiol by binding to estrogen receptor 1 reduces the expression of the Hey1 effector gene. In contrast to nuclear estrogen receptors, no evident role of G protein-coupled estrogen receptor 1 in regulating Notch1 signaling activity in Sertoli cells has been found. The data presented indicate the role of nuclear estrogen receptor signaling in the control of Notch1 pathway activity in Sertoli cells, and thus reveal new mechanism of estrogen action in maintaining homeostasis of the seminiferous epithelium.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sphingolipids and Male Reproductive Health: A Narrative Review of Their Roles in Spermatogenesis, Fertility, and Dysfunction.","authors":"Mojdeh Parvini, Fatemeh Ghasemian","doi":"10.1007/s43032-025-01898-4","DOIUrl":"https://doi.org/10.1007/s43032-025-01898-4","url":null,"abstract":"<p><p>Sphingolipids are bioactive lipids involved in cellular signaling and structural processes. Recent studies highlight their critical role in spermatogenesis, sperm functionality, and male infertility. Key sphingolipid metabolites, such as ceramide, ceramide-1-phosphate, and sphingosine-1-phosphate, mediate signaling pathways related to apoptosis, proliferation, stress responses, necrosis, inflammation, autophagy, aging, and differentiation. This review aims to summarize recent advancements in understanding the roles of sphingolipids in spermatogenesis, sperm motility, capacitation, the acrosome reaction, and fertilization, as well as their impact on abnormal spermatogenesis, gonadal dysfunction, and infertility. A narrative review was conducted by analyzing relevant literature published primarily between 2000 and 2024, focusing on the involvement of sphingolipids in male reproductive physiology. Sphingolipids regulate various stages of spermatogenesis, including germ cell proliferation, differentiation, and apoptosis, while also modulating critical processes such as sperm maturation, membrane remodeling, and signaling during capacitation and the acrosome reaction. Dysregulation of sphingolipid metabolism contributes to abnormal spermatogenesis, oxidative stress, testicular dysfunction, and impaired sperm functionality, which are linked to male infertility. Additionally, sphingolipids are implicated in sperm-oocyte fusion, further underscoring their essential role in male fertility. Understanding the precise mechanisms of sphingolipid action may offer potential therapeutic targets for treating male infertility. Future research should focus on elucidating these mechanisms and exploring interventions to correct sphingolipid imbalances.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass Spectrometric Analysis of Clomiphene Citrate-induced Changes in the Secretome Profile of PAX8-positive Human Fallopian Tube Secretory Epithelial Cells and Identification of Biomarkers Relevant to Reproduction and Pregnancy.","authors":"Opalina Roy, Shruthi Ammankallu, Vanya Kadla Narayana, Chinmaya Narayana, Shripad Hebbar, Prashanth K Adiga, Manjunath B Joshi, Jeevan Gowda, Anujith Kumar, Satish Kumar Adiga, Megan Ritting, Nagarajan Kannan, Thottethodi Subrahmanya Keshava Prasad, Guruprasad Kalthur","doi":"10.1007/s43032-025-01884-w","DOIUrl":"https://doi.org/10.1007/s43032-025-01884-w","url":null,"abstract":"<p><p>Clomiphene citrate (CC) is commonly used for infertility treatments, particularly in intrauterine insemination (IUI) and in vitro fertilization (IVF) programs. Despite its effectiveness in inducing ovulation, it is reported to have a low pregnancy rate compared to letrozole. Further, risks of ectopic pregnancy, miscarriage, and fetal malformations have been reported in CC-induced fertility treatment cycles. The present study aimed at understanding whether these effects are associated with CC-induced changes in the secretome profile of the human PAX8-positive fallopian tube secretory epithelial cells (hPFTSECs). Fallopian tube tissues from healthy women who underwent postpartum tubectomy were enzymatically dissociated and cultured with CC, and their secretomes were analyzed by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) to identify CC-induced alterations. Proteomic analysis identified a total of 1549 proteins in the secretome pool of hPFTSECs cultured in vitro and 258 proteins were found to be differentially abundant in response to CC. KEGG and REACTOME analysis revealed that the differentially abundant proteins (DAPs) were mainly associated with pathways such as estrogen signaling, cell cycle and DNA damage. Further, candidate proteins associated with ovulation, embryo development, embryo implantation, pregnancy complications, ectopic pregnancy, sperm functions, apoptosis, senescence, and ovarian cancers were identified. CC exposure induced changes in secretome profile of hPFTSECs may affect the female reproductive health by potentially altering the tubal microenvironment. The study was registered in the Clinical Trials Registry of India (CTRI) under the registration number CTRI/2020/11/028791 on 02/11/2020.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of xenobiotic-metabolizing gene cytochrome P450 2E1 variants with preeclampsia in Chinese women.","authors":"Kaifeng Hu, Qingqing Liu, Xinghui Liu, Huai Bai, Yujie Wu, Ping Fan","doi":"10.1007/s43032-025-01890-y","DOIUrl":"https://doi.org/10.1007/s43032-025-01890-y","url":null,"abstract":"<p><p>Environmental and genetic factors are related to the pathogenesis of preeclampsia (PE). Cytochrome P450 2E1 (CYP2E1) is crucial for the metabolism of endogenous and xenobiotic substances, possibly involved in the pathophysiology of PE. This study explored the association between CYP2E1 96-bp insertion/deletion (I/D) and rs2031920 (C-1054T) genetic variants and the risk of PE in 335 patients with PE and 1301 healthy pregnant women. The CYP2E1 C-1054T variant was linked to an elevated risk of PE according to the dominant, genotype, and allele genetic models (P < 0.05). The genotype TT + CT remained a significant predictor of PE in the logistic regression model including age, gestational age, and body mass index at delivery (OR = 1.606, 95% CI: 1.137-2.286; P = 0.007). Moreover, the combined genotype TT + CT/II + ID of the C-1054T and 96-bp I/D variants further heightened the risk of PE, with the combined genotype DD/CC serving as the reference category (OR = 2.383, 95% CI: 1.381-4.106, P = 0.002). Furthermore, patients with the -1054T allele had lower serum albumin levels and total antioxidant capacity, and more severe proteinuria than those with the CC genotype (P < 0.05), and patients with the 96-bp I allele had a relatively higher atherosclerosis index than those with the DD genotype (P = 0.057). No significant differences in genotype frequencies of PE with severe features, platelet count, serum hepatic enzyme activities and creatinine levels were observed according to the different genotypes (P > 0.05). We conclude that the T allele of the C-1054T variant and its integration with the I allele of the 96-bp I/D variant in CYP2E1 are linked to an elevated risk of PE in Chinese women.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage Plasticity and Extracellular Vesicles in Endometriosis: Immunopathology and Therapeutic Perspectives.","authors":"Saba Safdarpour, Parisa Nadri, Nima Azari-Dolatabad","doi":"10.1007/s43032-025-01896-6","DOIUrl":"https://doi.org/10.1007/s43032-025-01896-6","url":null,"abstract":"<p><p>Endometriosis, as a chronic gynecological condition, includes irregular endometrial cell growth beyond the uterus, which creates pelvic pain and infertility, together with inflammatory disease throughout the body. Currently, about 10% of all women worldwide experience this disease, but scientists have not determined its natural causes. Importantly, the development of endometriosis depends significantly on the dysfunction of the immune system, which results in impaired macrophage activity. Specifically, endometriotic lesions contain M2-like macrophages, supporting tissue reconstruction along new blood vessel formation and evading immune responses. Moreover, the survival of lesions and the processes of neurogenesis and inflammation depend on the actions of macrophage-derived extracellular vesicles (EVs) as well as cytokines and growth factors. In this context, this review investigates the process of macrophage polarization and details the cellular interactions found in endometriotic tissue environments and their therapeutic capacity. Ultimately, understanding the underlying mechanisms of disease will facilitate the development of immunomodulatory therapies aimed at reducing symptoms and improving clinical outcomes.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Human Myometrial Transcriptome and the DNA Methylome of Testosterone-treated Patients Resemble the Myometria from Fibroid Patients.","authors":"Emmanuel N Paul, Tyler J Carpenter, Andrew Bossick, Ghassan Allo, Ganesa R Wegienka, Jose M Teixeira","doi":"10.1007/s43032-025-01893-9","DOIUrl":"https://doi.org/10.1007/s43032-025-01893-9","url":null,"abstract":"<p><p>Uterine fibroids, or leiomyomas, are noncancerous tumors of the myometrium and the most common tumors in women, with a cumulative incidence of approximately 80% by age 50. Currently, hysterectomy is the only definitive cure, and effective non-hormonal therapeutics are lacking. Understanding the etiology of fibroids may lead to alternative, less invasive treatments. Several obstetric disorders, including polycystic ovary syndrome (PCOS), have been linked to uterine fibroids, and women with PCOS often exhibit hormonal imbalances, particularly elevated serum testosterone levels. However, the impact of testosterone on the myometrium remains poorly understood. We hypothesize that elevated testosterone may increase the risk of developing uterine fibroids. Using RNA sequencing and MethylationEPIC array analyses, we compared myometrial tissue from women without fibroids (MyoN, n = 33), with fibroids (MyoF, n = 66), and after testosterone therapy as part of clinical care for gender dysphoria (MyoT, n = 7). The transcriptomic and methylation profiles of MyoT clustered with MyoF and were distinct from MyoN. We identified 1,321 differentially expressed protein-coding genes between MyoT and MyoN, while only 494 were found between MyoT and MyoF. Disease ontology analysis of MyoT vs. MyoN revealed enrichment of the fibroid tumor gene set. Fibroid associated genes including TGFβ3, CCND1, SERPINE1, and FGFR1 were upregulated in MyoT and MyoF samples compared to MyoN samples. The DNA methylation profiles of MyoT were closer to those of MyoF, but no correlation was observed between methylation status and gene expression. Our preliminary data suggest that exogenous testosterone induces transcriptional and methylation changes in the myometrium consistent with those observed in MyoF tissues. These findings suggest that elevated testosterone may be associated with an increased risk of developing uterine fibroids.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}