RPL7和RCCD1作为薄子宫内膜患者免疫浸润相关的潜在生物标志物的鉴定和验证

IF 2.5 3区 医学 Q2 OBSTETRICS & GYNECOLOGY
Reproductive Sciences Pub Date : 2025-07-01 Epub Date: 2025-06-02 DOI:10.1007/s43032-025-01883-x
Jiani Sun, Mei Ji, Yiping Zhu, Orhan Bukulmez, Yeung William Shu-Biu, Jing Sun, Xiaoming Teng, Miaoxin Chen
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引用次数: 0

摘要

子宫内膜薄(TE)是导致胚胎着床不良和女性不孕症的重要因素,但其发病机制尚不清楚,限制了有效的治疗选择。为了鉴定TE的潜在相关基因并探索临床治疗的潜在机制,我们对18个TE和18个正常子宫内膜样本进行了RNA测序,然后进行了生物信息学分析。从样品中提取总RNA,反转录成cDNA,在HiSeq 2500平台上测序,然后进行差异表达分析和差异表达基因(DEGs)的功能富集。功能富集和LASSO分析确定了关键的生物标志物,随后在mRNA和蛋白质水平上进行了验证。免疫浸润模式和与免疫细胞的相关性也被检查。因此,核糖体蛋白L7 (RPL7)和RCC1结构域1 (RCCD1)被确定为潜在的生物标志物,在TE中过度表达并与异常免疫调节相关。这些发现表明,RPL7和RCCD1可能作为TE的特异性生物标志物,有助于开发靶向治疗方法以改善临床结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and Validation of RPL7 and RCCD1 as Potential Biomarkers Associated with Immune Infiltration in Patients with Thin Endometrium.

Thin endometriumQuery (TE) significantly contributes to poor embryo implantation and female infertility, yet its pathogenesis remains unclear, limiting effective treatment options. To identify potential TE-associated genes and explore underlying mechanisms for clinical therapy, we conducted RNA sequencing on 18 TE and 18 normal endometrium samples, followed by bioinformatics analysis. Total RNA was extracted from samples, reverse-transcribed into cDNA, and sequenced on the HiSeq 2500 platform, followed by differential expression analysis and functional enrichment of differentially expressed genes (DEGs). Functional enrichment and LASSO analysis identified key biomarkers, which were subsequently validated at both mRNA and protein levels. Immune infiltration patterns and correlations with immune cells were also examined. Consequently, ribosomal protein L7 (RPL7) and RCC1 domain-containing 1 (RCCD1) were identified as potential biomarkers, showing overexpression in TE and association with abnormal immune regulation. These findings suggest that RPL7 and RCCD1 may serve as specific biomarkers for TE, providing insights that could aid in developing targeted therapeutics to improve clinical outcomes.

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来源期刊
Reproductive Sciences
Reproductive Sciences 医学-妇产科学
CiteScore
5.50
自引率
3.40%
发文量
322
审稿时长
4-8 weeks
期刊介绍: Reproductive Sciences (RS) is a peer-reviewed, monthly journal publishing original research and reviews in obstetrics and gynecology. RS is multi-disciplinary and includes research in basic reproductive biology and medicine, maternal-fetal medicine, obstetrics, gynecology, reproductive endocrinology, urogynecology, fertility/infertility, embryology, gynecologic/reproductive oncology, developmental biology, stem cell research, molecular/cellular biology and other related fields.
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