{"title":"胚胎结局、胚胎移植后的活产结局和第一次细胞分裂过程中不同直接分裂时间定义的整倍体率:一项单中心、大队列、回顾性研究。","authors":"Satoshi Ueno, Shin Katakura, Tadashi Okimura, Keiichi Kato","doi":"10.1007/s43032-025-01842-6","DOIUrl":null,"url":null,"abstract":"<p><p>Although essential to confirm the clinical utility of direct cleavage embryos, the timing of rapid cleavage (RpiC) has not been specifically defined. This study aimed to explore the differences in embryonic and clinical outcomes based on varying timing parameters of direct cleavage during first cytokinesis, using time to reach the three-cell stage (t3) minus time to reach the two-cell stage (t2). We analyzed 19,796 fertilized embryos (in 6,907 patients) from a single center between September 2019 and December 2020. The embryos were cultured using EmbryoScope, and t2 and t3 were recorded. Trichotomous mitosis (TM) was defined as t3 - t2 = 0 h, and RpiC events were divided into four groups, as follows: 0 h < t3 - t2 < 1 h (RpiC-1), 1 h ≤ t3 - t2 < 3 h (RpiC-3), 3 h ≤ t3 - t2 < 5 h (RpiC-5), and 5 h ≤ t3 - t2 < 7 h (RpiC-7). Additionally, 7 h ≤ t3 - t2 < 14 h was defined as normal cleavage. After single-cleavage embryo transfer, the live-birth, TM, and RpiC-1 rates were significantly lower than those in other groups. Similarly, when blastocysts were utilized, the TM and RpiC-1 rates were significantly lower than those in other groups. This study suggests that embryos with TM or RpiC-1 (t3 - t2 < 1 h) should be cultured to the blastocyst stage to prevent unnecessary embryo transfers, although outcomes may vary in different scenarios, i.e., by institution and patient.</p>","PeriodicalId":20920,"journal":{"name":"Reproductive Sciences","volume":" ","pages":"1876-1885"},"PeriodicalIF":2.6000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Embryonic Outcomes, Live-Birth Outcomes After Embryo Transfer, and Euploid Rates for Various Direct Cleavage Timing Definitions During First Cytokinesis: A Single-Center, Large-Cohort, Retrospective Study.\",\"authors\":\"Satoshi Ueno, Shin Katakura, Tadashi Okimura, Keiichi Kato\",\"doi\":\"10.1007/s43032-025-01842-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Although essential to confirm the clinical utility of direct cleavage embryos, the timing of rapid cleavage (RpiC) has not been specifically defined. This study aimed to explore the differences in embryonic and clinical outcomes based on varying timing parameters of direct cleavage during first cytokinesis, using time to reach the three-cell stage (t3) minus time to reach the two-cell stage (t2). We analyzed 19,796 fertilized embryos (in 6,907 patients) from a single center between September 2019 and December 2020. The embryos were cultured using EmbryoScope, and t2 and t3 were recorded. Trichotomous mitosis (TM) was defined as t3 - t2 = 0 h, and RpiC events were divided into four groups, as follows: 0 h < t3 - t2 < 1 h (RpiC-1), 1 h ≤ t3 - t2 < 3 h (RpiC-3), 3 h ≤ t3 - t2 < 5 h (RpiC-5), and 5 h ≤ t3 - t2 < 7 h (RpiC-7). Additionally, 7 h ≤ t3 - t2 < 14 h was defined as normal cleavage. After single-cleavage embryo transfer, the live-birth, TM, and RpiC-1 rates were significantly lower than those in other groups. Similarly, when blastocysts were utilized, the TM and RpiC-1 rates were significantly lower than those in other groups. This study suggests that embryos with TM or RpiC-1 (t3 - t2 < 1 h) should be cultured to the blastocyst stage to prevent unnecessary embryo transfers, although outcomes may vary in different scenarios, i.e., by institution and patient.</p>\",\"PeriodicalId\":20920,\"journal\":{\"name\":\"Reproductive Sciences\",\"volume\":\" \",\"pages\":\"1876-1885\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproductive Sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s43032-025-01842-6\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive Sciences","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s43032-025-01842-6","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/3 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
尽管对直接卵裂胚胎的临床应用至关重要,但快速卵裂(RpiC)的时间尚未明确定义。本研究旨在探讨在第一次细胞分裂过程中不同的直接分裂时间参数对胚胎和临床结果的差异,用到达三细胞期(t3)的时间减去到达两细胞期(t2)的时间。我们分析了2019年9月至2020年12月期间来自单个中心的19796个受精胚胎(6907名患者)。胚胎在EmbryoScope下培养,记录t2和t3。将三分体有丝分裂(TM)定义为t3 - t2 = 0 h,将RpiC事件分为4组,分别为:0 h
Embryonic Outcomes, Live-Birth Outcomes After Embryo Transfer, and Euploid Rates for Various Direct Cleavage Timing Definitions During First Cytokinesis: A Single-Center, Large-Cohort, Retrospective Study.
Although essential to confirm the clinical utility of direct cleavage embryos, the timing of rapid cleavage (RpiC) has not been specifically defined. This study aimed to explore the differences in embryonic and clinical outcomes based on varying timing parameters of direct cleavage during first cytokinesis, using time to reach the three-cell stage (t3) minus time to reach the two-cell stage (t2). We analyzed 19,796 fertilized embryos (in 6,907 patients) from a single center between September 2019 and December 2020. The embryos were cultured using EmbryoScope, and t2 and t3 were recorded. Trichotomous mitosis (TM) was defined as t3 - t2 = 0 h, and RpiC events were divided into four groups, as follows: 0 h < t3 - t2 < 1 h (RpiC-1), 1 h ≤ t3 - t2 < 3 h (RpiC-3), 3 h ≤ t3 - t2 < 5 h (RpiC-5), and 5 h ≤ t3 - t2 < 7 h (RpiC-7). Additionally, 7 h ≤ t3 - t2 < 14 h was defined as normal cleavage. After single-cleavage embryo transfer, the live-birth, TM, and RpiC-1 rates were significantly lower than those in other groups. Similarly, when blastocysts were utilized, the TM and RpiC-1 rates were significantly lower than those in other groups. This study suggests that embryos with TM or RpiC-1 (t3 - t2 < 1 h) should be cultured to the blastocyst stage to prevent unnecessary embryo transfers, although outcomes may vary in different scenarios, i.e., by institution and patient.
期刊介绍:
Reproductive Sciences (RS) is a peer-reviewed, monthly journal publishing original research and reviews in obstetrics and gynecology. RS is multi-disciplinary and includes research in basic reproductive biology and medicine, maternal-fetal medicine, obstetrics, gynecology, reproductive endocrinology, urogynecology, fertility/infertility, embryology, gynecologic/reproductive oncology, developmental biology, stem cell research, molecular/cellular biology and other related fields.