IF 2.5 3区生物学

Protist Pub Date : 2024-02-01 DOI: 10.1016/j.protis.2024.126016

Leah Lourenço , Sara Ellegaard Bager , Duncan Y.K. Ng , Sanea Sheikh , Nikolaj Lunding Kindtler , Ida Broman Nielsen , Tobias Guldberg Frøslev , Flemming Ekelund

{"title":"DNA metabarcoding reveals the impact of Cu2+ on soil cercozoan diversity","authors":"Leah Lourenço , Sara Ellegaard Bager , Duncan Y.K. Ng , Sanea Sheikh , Nikolaj Lunding Kindtler , Ida Broman Nielsen , Tobias Guldberg Frøslev , Flemming Ekelund","doi":"10.1016/j.protis.2024.126016","DOIUrl":"10.1016/j.protis.2024.126016","url":null,"abstract":"<div>Although copper (Cu2+) is a micronutrient, the metal may be toxic if present in high concentrations in soil ecosystems and subsequently affect various organisms, ranging from microorganisms to earthworms. We performed a microcosm study with an array of Cu2+ concentrations, with a specific focus on Cercozoa, an important protozoan group in most soil food webs. Research on Cercozoa is still scarce in terms of both diversity and ecology; hence, to explore this group in more depth, we used high-throughput sequencing to detect Cu2+ induced community changes. Increased levels of Cu2+ caused a shift in the cercozoan community, and we observed decreased cercozoan relative abundance across the majority of orders, families and genera. Due to their key role in soil food webs, especially as bacterial predators and providers of nutrients to plants, the reduction of cercozoan abundance and diversity may seriously affect soil functionality. Our results indicate that the increase of Cu2+ concentrations in the soil could potentially have this effect and the consequences need exploration.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461024000087/pdfft?md5=4fe633e138ca7012ea67f8d32d371e4c&pid=1-s2.0-S1434461024000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic affiliation of Pedinomonas noctilucae and green Noctiluca scintillans nutritional dynamics in the Gulf of Mannar, Southeastern Arabian Sea 阿拉伯海东南部马纳尔湾夜褐孔雀藻和闪烁绿夜光藻营养动态的系统发育隶属关系

IF 2.5 3区生物学

Protist Pub Date : 2024-01-30 DOI: 10.1016/j.protis.2024.126019

Vajravelu Manigandan, Chandrasekaran Muthukumar, Chinmay Shah, Natarajan Logesh, Sanitha K. Sivadas, Karri Ramu, M.V. Ramana Murthy

{"title":"Phylogenetic affiliation of Pedinomonas noctilucae and green Noctiluca scintillans nutritional dynamics in the Gulf of Mannar, Southeastern Arabian Sea","authors":"Vajravelu Manigandan, Chandrasekaran Muthukumar, Chinmay Shah, Natarajan Logesh, Sanitha K. Sivadas, Karri Ramu, M.V. Ramana Murthy","doi":"10.1016/j.protis.2024.126019","DOIUrl":"10.1016/j.protis.2024.126019","url":null,"abstract":"<div>The present investigation focused on studying the phylogenetic position of the green Noctiluca endosymbiont, Pedinomonas noctilucae, collected from the Gulf of Mannar, India. In this study, we re-examined the evolutionary position of this endosymbiotic algae using rbcL sequences. The phylogenetic analysis revealed that P. noctilucae is distantly related to the Pedinomonas species, and formed a monophyletic clade with Marsupiomandaceae. Based on the phylogenetic association of endosymbiont with Maruspiomonadales it was concluded that the endosymbiont belongs to an independent genus within the family Marsupiomonadaceae. At the site of the bloom, Noctiluca scintillans was found to exhibit a dense monospecific proliferation, with an average cell density of 27.l88 × 103 cells L−1. The investigation revealed that the green Noctiluca during its senescent phase primarily relied on autotrophic nutrition, which was confirmed by the presence of a high number of trophonts, vegetatively reproducing cells (1.45 × 103 cells L−1) and the absence of food vacuoles.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139646007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of a new photosynthetic euglenoid in Poland: Euglena mazurica sp. nov. (Euglenales, Euglenaceae) 在波兰发现一种新的光合鳗：Euglena mazurica sp.

IF 2.5 3区生物学

Protist Pub Date : 2024-01-28 DOI: 10.1016/j.protis.2024.126015

Bożena Zakryś, Katarzyna Jankowska, Anna Majerowicz, Alicja Fells, Maja Łukomska-Kowalczyk

引用次数: 0

Ras superfamily GTPases and signal transduction in Euglena gracilis Ras 超家族 GTP 酶与草履虫的信号转导

IF 2.5 3区生物学

Protist Pub Date : 2024-01-28 DOI: 10.1016/j.protis.2024.126017

Mark C. Field

{"title":"Ras superfamily GTPases and signal transduction in Euglena gracilis","authors":"Mark C. Field","doi":"10.1016/j.protis.2024.126017","DOIUrl":"10.1016/j.protis.2024.126017","url":null,"abstract":"<div>Biological complexity is challenging to define, but can be considered through one or more features, including overall genome size, number of genes, morphological features, multicellularity, number of life cycle stages and the ability to adapt to different environments. Euglena gracilis meets several of these criteria, with a large genome of ∼38,000 protein coding genes and a considerable ability to survive under many different conditions, some of which can be described as challenging or harsh. Potential molecular exemplars of complexity tying these aspects together are signalling pathways, including GTPases, kinases and ubiquitylation, which increase the functionality of the gene-encoded proteome manyfold. Each of these examples can modulate both protein activity and gene expression. To address the connection between genome size and complexity I have undertaken a brief, and somewhat qualitative, survey of the small ras-like GTPase superfamily of E. gracilis. Unexpectedly, apart from Rab-GTPases which control intracellular transport and organelle identify, the size of the GTPase cohort is modest, and, for example, has not scaled with gene number when compared to the close relatives, trypanosomatids. I suggest that understanding the functions of this protein family will be vital to uncovering the complexity of E. gracilis biology.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461024000099/pdfft?md5=d23c2ce3f9eb244c429ad172b9528154&pid=1-s2.0-S1434461024000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139590215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell genomics revealed Candidatus Grellia alia sp. nov. as an endosymbiont of Eutreptiella sp. (Euglenophyceae) 单细胞基因组学揭示了作为Eutreptiella sp.（Euglenophyceae）内共生体的Candidatus Grellia alia sp.

IF 2.5 3区生物学

Protist Pub Date : 2024-01-27 DOI: 10.1016/j.protis.2024.126018

Metody Hollender, Marta Sałek, Michał Karlicki, Anna Karnkowska

{"title":"Single-cell genomics revealed Candidatus Grellia alia sp. nov. as an endosymbiont of Eutreptiella sp. (Euglenophyceae)","authors":"Metody Hollender, Marta Sałek, Michał Karlicki, Anna Karnkowska","doi":"10.1016/j.protis.2024.126018","DOIUrl":"10.1016/j.protis.2024.126018","url":null,"abstract":"<div>Though endosymbioses between protists and prokaryotes are widespread, certain host lineages have received disproportionate attention what may indicate either a predisposition to such interactions or limited studies on certain protist groups due to lack of cultures. The euglenids represent one such group in spite of microscopic observations showing intracellular bacteria in some strains. Here, we perform a comprehensive molecular analysis of a previously identified endosymbiont in the Eutreptiella sp. CCMP3347 using a single cell approach and bulk culture sequencing. The genome reconstruction of this endosymbiont allowed the description of a new endosymbiont Candidatus Grellia alia sp. nov. from the family Midichloriaceae. Comparative genomics revealed a remarkably complete conjugative type IV secretion system present in three copies on the plasmid sequences of the studied endosymbiont, a feature missing in the closely related Grellia incantans. This study addresses the challenge of limited host cultures with endosymbionts by showing that the genomes of endosymbionts reconstructed from single host cells have the completeness and contiguity that matches or exceeds those coming from bulk cultures. This paves the way for further studies of endosymbionts in euglenids and other protist groups. The research also provides the opportunity to study the diversity of endosymbionts in natural populations.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461024000105/pdfft?md5=dd511c0b822f3e967d63e6ba4dc1dfc7&pid=1-s2.0-S1434461024000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139590216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On Saville-Kent's “a Manual of the infusoria” 关于萨维尔-肯特的 "输卵管手册"

IF 2.5 3区生物学

Protist Pub Date : 2023-12-23 DOI: 10.1016/j.protis.2023.126008

John R. Dolan

引用次数: 0

Morphogenesis of an anaerobic ciliate Heterometopus palaeformis (Kahl, 1927) Foissner, 2016 (Ciliophora, Armophorea) with notes on its morphological and molecular characterization 厌氧纤毛虫 Heterometopus palaeformis (Kahl, 1927) Foissner, 2016（纤毛虫，腕足动物）的形态发生及其形态和分子特征说明

IF 2.5 3区生物学

Protist Pub Date : 2023-12-16 DOI: 10.1016/j.protis.2023.126007

Wenbao Zhuang , Xiaochen Feng , Ran Li , Saleh A. Al-Farraj , Xiaozhong Hu

{"title":"Morphogenesis of an anaerobic ciliate Heterometopus palaeformis (Kahl, 1927) Foissner, 2016 (Ciliophora, Armophorea) with notes on its morphological and molecular characterization","authors":"Wenbao Zhuang , Xiaochen Feng , Ran Li , Saleh A. Al-Farraj , Xiaozhong Hu","doi":"10.1016/j.protis.2023.126007","DOIUrl":"10.1016/j.protis.2023.126007","url":null,"abstract":"<div>The morphology, morphogenesis, and molecular phylogeny of Heterometopus palaeformis (Kahl, 1927) Foissner, 2016 were studied using microscopical observations on live and protargol-stained specimens as well SSU rRNA gene sequencing. The morphogenetic data for the genus are presented for the first time. Compared to other metopids, the morphogenesis of H. palaeformis is distinct since its (1) perizonal stripe rows 4 and 5 are involved in the formation of the opisthe’s adoral polykinetids; (2) perizonal stripe rows 3–5 and two adjacent preoral dome kineties contribute to most of the opisthe’s paroral membrane while perizonal stripe rows 1 and 2 contribute very little; (3) four kinety rows are formed to the left of the opisthe’s adoral zone of polykinetids. The Chinese population resembles the original and neotype populations well in terms of general morphology — characterized by a life size of 55–120 × 10–20 μm, an elongate ellipsoidal body with a hardly spiralized flat preoral dome, about 18 somatic kineties and 20 adoral polykinetids. The SSU rDNA sequence of the present population exhibits a disparity of 1.33%–2.22% divergence from sequences of other populations. Nevertheless, phylogenetic analysis reveals that populations of H. palaeformis form a separate, stable cluster within the paraphyletic Metopidae clade.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138743209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting algicidal efficiency of Alteromonas sp. FDHY-CJ against Skeletonema costatum through fermentation optimization 通过发酵优化提高 Alteromonas sp. FDHY-CJ 对 Skeletonema costatum 的杀菌效率

IF 2.5 3区生物学

Protist Pub Date : 2023-12-12 DOI: 10.1016/j.protis.2023.126006

Pin Lv , Xinguo Shi , Qianqian Wang , Yuying Zhong , Yisong Guo , Jianfeng Chen

{"title":"Boosting algicidal efficiency of Alteromonas sp. FDHY-CJ against Skeletonema costatum through fermentation optimization","authors":"Pin Lv , Xinguo Shi , Qianqian Wang , Yuying Zhong , Yisong Guo , Jianfeng Chen","doi":"10.1016/j.protis.2023.126006","DOIUrl":"10.1016/j.protis.2023.126006","url":null,"abstract":"<div>Algicidal bacteria exhibit promising potential against harmful algal blooms (HABs); however, their application has been limited due to their limited algicidal activity. This study demonstrates the enhanced algicidal activity of Alteromonas sp. FDHY-CJ bacteria against harmful Skeletonema costatum using a 5 L fermenter. Utilizing this refined framework increased the OD600 value and algal cell mortality by 6.50 and 2.88 times, respectively, compared to non-optimized culture cultivated in a flask using marine broth 2216E medium. The mechanism of action involves significant inhibition of algal photosynthetic efficiency with concurrent degradation of photosynthetic pigments. Relative to the non-optimized group, the optimized bacterial treatment led to a significant increase in H2O2 and MDA (malondialdehyde) by 19.54 and 4.22-fold, respectively, and resulted in membrane damage. The culture optimization procedure yielded effectual algicidal substances capable of considerably reducing the severity of S. costatum HABs through cell membrane disruption.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138580168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gleaning Euglenozoa-specific DNA polymerases in public single-cell transcriptome data 在公开的单细胞转录组数据中收集真核生物特异性DNA聚合酶。

IF 2.5 3区生物学

Protist Pub Date : 2023-12-01 DOI: 10.1016/j.protis.2023.125997

Ryo Harada , Yuji Inagaki

{"title":"Gleaning Euglenozoa-specific DNA polymerases in public single-cell transcriptome data","authors":"Ryo Harada , Yuji Inagaki","doi":"10.1016/j.protis.2023.125997","DOIUrl":"10.1016/j.protis.2023.125997","url":null,"abstract":"<div>Multiple genes encoding family A DNA polymerases (famA DNAPs), which are evolutionary relatives of DNA polymerase I (PolI) in bacteria and phages, have been found in eukaryotic genomes, and many of these proteins are used mainly in organelles. Among members of the phylum Euglenozoa, distinct types of famA DNAP, PolIA, PolIBCD+, POP, and eugPolA, have been found. It is intriguing how the suite of famA DNAPs had been established during the evolution of Euglenozoa, but the DNAP data have not been sampled from the taxa that sufficiently represent the diversity of this phylum. In particular, little sequence data were available for basal branching species in Euglenozoa until recently. Thanks to the single-cell transcriptome data from symbiontids and phagotrophic euglenids, we have an opportunity to cover the “hole” in the repertory of famA DNAPs in the deep branches in Euglenozoa. The current study identified 16 new famA DNAP sequences in the transcriptome data from 33 phagotrophic euglenids and two symbiontids, respectively. Based on the new famA DNAP sequences, the updated diversity and evolution of famA DNAPs in Euglenozoa are discussed.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138470717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding wax ester synthesis in Euglena gracilis: Insights into mitochondrial anaerobic respiration 了解薄叶草蜡酯合成:线粒体厌氧呼吸的见解

IF 2.5 3区生物学

Protist Pub Date : 2023-12-01 DOI: 10.1016/j.protis.2023.125996

Masami Nakazawa , Hiroshi Inui

{"title":"Understanding wax ester synthesis in Euglena gracilis: Insights into mitochondrial anaerobic respiration","authors":"Masami Nakazawa , Hiroshi Inui","doi":"10.1016/j.protis.2023.125996","DOIUrl":"https://doi.org/10.1016/j.protis.2023.125996","url":null,"abstract":"<div>Euglena gracilis, photosynthetic protist, has a unique ability to generate wax esters in the absence of oxygen, employing a distinctive fatty acid synthesis mechanism. Through comprehensive inhibitor assays and gene-silencing techniques, our research clearly emphasized the indispensable role of the mitochondrial anaerobic respiratory chain in this biosynthesis. We identified acyl-CoA dehydrogenase, electron transfer flavoprotein (ETF), and rhodoquinone (RQ) as central molecular components in the pathway. These findings strongly indicated a potential reversal of beta-oxidation occurring within mitochondria for fatty acid production in anaerobic conditions. Furthermore, our analysis revealed the pivotal function of nicotinamide nucleotide transhydrogenase (NNT) in efficiently managing the NADPH/NAD+ conversion essential for sustaining anaerobic metabolism. This review outlines our key findings and provides a comprehensive understanding of the molecular mechanisms that enable E. gracilis to produce wax ester anaerobically.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138471651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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