IF 2.5 3区生物学

Protist Pub Date : 2023-11-08 DOI: 10.1016/j.protis.2023.125995

Juan Rodero Madrid , José Luis Olmo , Genoveva F. Esteban

{"title":"First record of Colpodidium caudatum (Ciliophora, Colpodidiidae) in Europe","authors":"Juan Rodero Madrid , José Luis Olmo , Genoveva F. Esteban","doi":"10.1016/j.protis.2023.125995","DOIUrl":"10.1016/j.protis.2023.125995","url":null,"abstract":"<div>The present study documents the discovery of the first European population of Colpodidium caudatum (Ciliophora, Colpodidiidae) in a water drain in a school playground in Manzanares (Ciudad Real, Spain). This species has been documented on every continent except Antarctica and Europe, until now. The ciliate was isolated from wet run-off soil collected from the water drain and was grown in semi-permanent cultures in the laboratory. The infraciliature of the ciliate was revealed using silver carbonate impregnation and cell measurements were taken from living and silver-impregnated specimens. A comparative analysis of published data from various populations of C. caudatum across the globe showed high intraspecific morphological variability in this species. To differentiate between species within the Colpodidium genus, a dichotomous key is presented. This investigation shows that C. caudatum is a ciliate that is found all over the world and is particularly associated with terrestrial habitats that are periodically flooded.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1434461023000573/pdfft?md5=0b4c309713a1303141c73cafeb2ab4fc&pid=1-s2.0-S1434461023000573-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135514634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A three-genome ultraconserved element phylogeny of cryptophytes 隐生植物的三基因组超保守元件系统发育。

IF 2.5 3区生物学

Protist Pub Date : 2023-11-02 DOI: 10.1016/j.protis.2023.125994

Matthew J. Greenwold , Kristiaän Merritt , Tammi L. Richardson , Jeffry L. Dudycha

{"title":"A three-genome ultraconserved element phylogeny of cryptophytes","authors":"Matthew J. Greenwold , Kristiaän Merritt , Tammi L. Richardson , Jeffry L. Dudycha","doi":"10.1016/j.protis.2023.125994","DOIUrl":"10.1016/j.protis.2023.125994","url":null,"abstract":"<div>Cryptophytes are single celled protists found in all aquatic environments. They are composed of a heterotrophic genus, Goniomonas, and a largely autotrophic group comprising many genera. Cryptophytes evolved through secondary endosymbiosis between a host eukaryotic heterotroph and a symbiont red alga. This merger resulted in a four-genome system that includes the nuclear and mitochondrial genomes from the host and a second nuclear genome (nucleomorph) and plastid genome inherited from the symbiont. Here, we make use of different genomes (with potentially distinct evolutionary histories) to perform a phylogenomic study of the early history of cryptophytes. Using ultraconserved elements from the host nuclear genome and symbiont nucleomorph and plastid genomes, we produce a three-genome phylogeny of 91 strains of cryptophytes. Our phylogenetic analyses find that that there are three major cryptophyte clades: Clade 1 comprises Chroomonas and Hemiselmis species, Clade 2, a taxonomically rich clade, comprises at least twelve genera, and Clade 3, comprises the heterotrophic Goniomonas species. Each of these major clades include both freshwater and marine species, but subclades within these clades differ in degrees of niche conservatism. Finally, we discuss priorities for taxonomic revision to Cryptophyceae based on previous studies and in light of these phylogenomic analyses.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71485252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ciliates from the faeces of the free-ranging dromedary from Oman: Morphology and molecular phylogeny 阿曼自由放养单峰骆驼粪便中的纤毛虫：形态学和分子系统发育。

IF 2.5 3区生物学

Protist Pub Date : 2023-10-10 DOI: 10.1016/j.protis.2023.125993

Olga A. Kornilova , Anna I. Ganyukova , Maria E. Belokon , Vladimir V. Platonov , Ludmila V. Chistyakova

{"title":"Ciliates from the faeces of the free-ranging dromedary from Oman: Morphology and molecular phylogeny","authors":"Olga A. Kornilova , Anna I. Ganyukova , Maria E. Belokon , Vladimir V. Platonov , Ludmila V. Chistyakova","doi":"10.1016/j.protis.2023.125993","DOIUrl":"10.1016/j.protis.2023.125993","url":null,"abstract":"<div>Ciliates Infundibulorium cameli from the faeces of the free-ranging dromedary from Oman were studied using a set of methods of the light and immunofluorescence microscopy and molecular phylogeny. With the use of molecular genetic methods, it was confirmed that the cysts found in the samples simultaneously with trophozoites actually belong to the species I. cameli. Tubulin cytoskeleton organization of trophozoites and cysts of this species were described for the first time. A striking morphological similarity between species I. cameli and Buxtonella sulcata was demonstrated, including the organization of ciliature. Different isolates of I. cameli and B. sulcata formed a common clade on the phylogenetic tree. The level of evolutionary divergence between the 18 S rRNA sequences of I. cameli, B. sulcata and species closest to them according to the results of molecular phylogenetic analysis was estimated. It was demonstrated that the divergence between I. cameli and B. sulcata is extremely low compared to members of other genera included in the analysis. Taxonomic position of I. cameli and B. sulcata was discussed in according to the data of comparative morphology and molecular phylogeny</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41238099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomy and morphology of Calciopappus curvus sp. nov. (Syracosphaeraceae, Prymnesiophyceae), a novel appendage-bearing coccolithophore 一种新的附肢球石藻Calciopapus curvus sp.nov.的分类和形态。

IF 2.5 3区生物学

Protist Pub Date : 2023-10-01 DOI: 10.1016/j.protis.2023.125983

Odysseas A. Archontikis , Josué G. Millán , Harald Andruleit , Lluïsa Cros , Annelies Kleijne , Mikal Heldal , Hai Doan-Nhu , Amos Winter , Leocadio Blanco-Bercial , Jeremy R. Young

{"title":"Taxonomy and morphology of Calciopappus curvus sp. nov. (Syracosphaeraceae, Prymnesiophyceae), a novel appendage-bearing coccolithophore","authors":"Odysseas A. Archontikis , Josué G. Millán , Harald Andruleit , Lluïsa Cros , Annelies Kleijne , Mikal Heldal , Hai Doan-Nhu , Amos Winter , Leocadio Blanco-Bercial , Jeremy R. Young","doi":"10.1016/j.protis.2023.125983","DOIUrl":"10.1016/j.protis.2023.125983","url":null,"abstract":"<div>Based on scanning electron microscopy observations, a new species of the coccolithophore genus Calciopappus (Syracosphaeraceae, Prymnesiophyceae) is described from the surface waters off Bergen and from the lower photic zone of sub-tropical and tropical waters. Morphological, coccolith rim structure and biometric analyses strongly support separation of this morphotype from the two described Calciopappus species, but inclusion of it within the genus. The new form differs from the other species in being noticeably smaller and in morpho-structural details of each of the three coccolith types that form the coccosphere: (1) the body coccoliths have an open central area; (2) the whorl coccoliths have a wide central opening and two thumb-like protrusions; and (3) the appendage coccoliths are curved. On this basis, the species is formally described as Calciopappus curvus sp. nov., its systematic affinity is discussed and compared with other extant coccolithophores.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10603720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic architecture constrains macromolecular allocation in dinoflagellates 基因组结构限制了甲藻中大分子的分配。

IF 2.5 3区生物学

Protist Pub Date : 2023-09-12 DOI: 10.1016/j.protis.2023.125992

Olga Carnicer , Ying-Yu Hu , Vinitha Ebenezer , Andrew J. Irwin , Zoe V. Finkel

{"title":"Genomic architecture constrains macromolecular allocation in dinoflagellates","authors":"Olga Carnicer , Ying-Yu Hu , Vinitha Ebenezer , Andrew J. Irwin , Zoe V. Finkel","doi":"10.1016/j.protis.2023.125992","DOIUrl":"10.1016/j.protis.2023.125992","url":null,"abstract":"<div>Dinoflagellate genomes have a unique architecture that may constrain their physiological and biochemical responsiveness to environmental stressors. Here we quantified how nitrogen (N) starvation influenced macromolecular allocation and C:N:P of three photosynthetic marine dinoflagellates, representing different taxonomic classes and genome sizes. Dinoflagellates respond to nitrogen starvation by decreasing cellular nitrogen, protein and RNA content, but unlike many other eukaryotic phytoplankton examined RNA:protein is invariant. Additionally, 2 of the 3 species exhibit increases in cellular phosphorus and very little change in cellular carbon with N-starvation. As a consequence, N starvation induces moderate increases in C:N, but extreme decreases in N:P and C:P, relative to diatoms. Dinoflagellate DNA content relative to total C, N and P is much higher than similar sized diatoms, but similar to very small photosynthetic picoeukaryotes such as Ostreococcus. In aggregate these results indicate the accumulation of phosphate stores may be an important strategy employed by dinoflagellates to meet P requirements associated with the maintenance and replication of their large genomes.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41145851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomy and molecular phylogeny of pleurostomatid ciliates from China with a description of two new species 中国胸骨口虫的分类和分子系统发育及两新种记述

IF 2.5 3区生物学

Protist Pub Date : 2023-08-01 DOI: 10.1016/j.protis.2023.125975

Gongaote Zhang , Yuan Li , Ruitao Gong , Yu Qiao , Saleh A. Al-Farraj , Hongbo Pan , Zhe Wang , Hunter N. Hines

{"title":"Taxonomy and molecular phylogeny of pleurostomatid ciliates from China with a description of two new species","authors":"Gongaote Zhang , Yuan Li , Ruitao Gong , Yu Qiao , Saleh A. Al-Farraj , Hongbo Pan , Zhe Wang , Hunter N. Hines","doi":"10.1016/j.protis.2023.125975","DOIUrl":"10.1016/j.protis.2023.125975","url":null,"abstract":"<div>Ciliates in the order Pleurostomatida are found free-living in many habitats including within biofilms, but some (e.g. Pseudoamphileptus spp.) are ectocommensal on various hosts. Due to issues involving overall undersampling, the exact diversity and molecular phylogeny of this group remain largely underexplored. To combat this deficiency, detailed investigations were undertaken in northern China. As a result of these studies, we provide the morphological descriptions of two new species. Pseudoamphileptus apomacrostoma sp. nov., a new ectocommensal species, is characterized by the broadly oval cell shape, numerous scattered contractile vacuoles, and unique densely bounded extrusomes; Amphileptus qingdaoensis sp. nov., a marine form, is characterized by possessing oblong extrusomes with a conical anterior end, a single contractile vacuole and 5–7 left and 18–23 right kineties. In addition, a new population of Amphileptus orientalis Zhang et al., 2022, a freshwater representative, was documented and an improved diagnosis is provided. The phylogenetic analyses based on the SSU rDNA sequences imply that the genus Pseudoamphileptus is monophyletic whereas the genus Amphileptus is paraphyletic. The new molecular sequences presented here further support the establishment of two new species.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9918384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protozoa in the remarkable wall charts of Leuckart and Nitsche's Zoologische Wandtaflen 在琉卡特和尼采的《动物学报》的著名挂图里的原生动物

IF 2.5 3区生物学

Protist Pub Date : 2023-08-01 DOI: 10.1016/j.protis.2023.125974

John Richard Dolan

引用次数: 0

Taxonomy of a New Parasitic Euglenid, Euglenaformis parasitica sp. nov. (Euglenales, Euglenaceae) in Ostracods and Rhabdocoels 介形虫和横纹虫中一种新的寄生Euglenaformis parasitica sp. nov. (euglenae, Euglenaceae)的分类

IF 2.5 3区生物学

Protist Pub Date : 2023-08-01 DOI: 10.1016/j.protis.2023.125967

Koichiro Kato , Kensuke Yahata , Takeshi Nakayama

{"title":"Taxonomy of a New Parasitic Euglenid, Euglenaformis parasitica sp. nov. (Euglenales, Euglenaceae) in Ostracods and Rhabdocoels","authors":"Koichiro Kato , Kensuke Yahata , Takeshi Nakayama","doi":"10.1016/j.protis.2023.125967","DOIUrl":"10.1016/j.protis.2023.125967","url":null,"abstract":"<div>Parasitic euglenids have rarely been studied. We found parasitic euglenids in two species of ostracods (Cyprinotus cassidula, Dolerocypris sinensis) and two species of rhabdocoels (Mesostoma lingua, Microdalyellia armigera) in a rice field. These parasites grew and proliferated inside the host body. These parasites had pellicle strips, one emergent flagellum, and a red stigma, but no chloroplasts, and showed euglenoid movement. Inside the living host, they did not have emergent flagella and moved only by euglenoid movement, but when the host died or the parasites were isolated from the host, they extended their flagella and switched to swimming movement. We conclude that the parasites found in the four hosts that we examined are of the same species, considering the morphological characteristics and identities in the nSSU and nLSU rDNA sequences of those parasites. Molecular phylogenetic analysis showed that the parasite formed a clade with the free-living photoautotrophic species of Euglenaformis, with moderate statistical support. Therefore, the parasite is a secondary osmotroph derived from a photoautotrophic ancestor. Based on the results of morphological observation and molecular phylogenetic analysis, we propose a new species of parasitic euglenid, Euglenaformis parasitica sp. nov.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9918365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Diverse eukaryotic phytoplankton from around the Marquesas Islands documented by combined microscopy and molecular techniques 结合显微镜和分子技术记录了马克萨斯群岛周围的各种真核浮游植物

IF 2.5 3区生物学

Protist Pub Date : 2023-08-01 DOI: 10.1016/j.protis.2023.125965

Jana Veselá-Strejcová , Eleonora Scalco , Adriana Zingone , Sébastien Colin , Luigi Caputi , Diana Sarno , Jana Nebesářová , Chris Bowler , Julius Lukeš

{"title":"Diverse eukaryotic phytoplankton from around the Marquesas Islands documented by combined microscopy and molecular techniques","authors":"Jana Veselá-Strejcová , Eleonora Scalco , Adriana Zingone , Sébastien Colin , Luigi Caputi , Diana Sarno , Jana Nebesářová , Chris Bowler , Julius Lukeš","doi":"10.1016/j.protis.2023.125965","DOIUrl":"10.1016/j.protis.2023.125965","url":null,"abstract":"<div>Oceanic phytoplankton serve as a base for the food webs within the largest planetary ecosystem. Despite this, surprisingly little is known about species composition, function and ecology of phytoplankton communities, especially for vast areas of the open ocean. In this study we focus on the marine phytoplankton microflora from the vicinity of the Marquesas Islands in the Southern Pacific Ocean collected during the Tara Oceans expedition. Multiple samples from four sites and two depths were studied in detail using light microscopy, scanning electron microscopy, and automated confocal laser scanning microscopy. In total 289 taxa were identified, with Dinophyceae and Bacillariophyceae contributing 60% and 32% of taxa, respectively, to phytoplankton community composition. Notwithstanding, a large number of cells could not be assigned to any known species. Coccolithophores and other flagellates together contributed less than 8% to the species list. Observed cell densities were generally low, but at sites of high autotrophic biomass, diatoms reached the highest cell densities (1.26 × 104 cells L−1). Overall, 18S rRNA metabarcode-based community compositions matched microscopy-based estimates, particularly for the main diatom taxa, indicating consistency and complementarity between different methods, while the wide range of microscopy-based methods permitted several unknown and poorly studied taxa to be revealed and identified.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Mayorella Species Isolated from the Mariana Trench Area (Pacific Ocean) 太平洋马里亚纳海沟一新种

IF 2.5 3区生物学

Protist Pub Date : 2023-06-01 DOI: 10.1016/j.protis.2023.125958

Xiaoli Lei , Xiaojuan Chen , Jianming Chen , Chen Liang

{"title":"A New Mayorella Species Isolated from the Mariana Trench Area (Pacific Ocean)","authors":"Xiaoli Lei , Xiaojuan Chen , Jianming Chen , Chen Liang","doi":"10.1016/j.protis.2023.125958","DOIUrl":"10.1016/j.protis.2023.125958","url":null,"abstract":"<div>In this paper, we describe a new naked amoeba species, Mayorella marianaensis sp. n., order Dermamoebida, isolated from the bottom of the Pacific Ocean (>3,000 m depth) in the vicinity of the Mariana Trench, based on morphological and molecular data. The newly discovered species was identified based on morphological and molecular data. This is the first time that a Mayorella species was discovered in the deep sea (>1,000 m). Mayorella marianaensis is an irregularly rectangular naked amoeba (30–120 × 11–60 µm), with a narrow frontal hyaline area. Four to 15 conical sub-pseudopodia, and three kinds of floating forms are identified. Trophozoites have a thick cell coat consisting of two distinct layers. The small subunit ribosomal RNA gene phylogeny showed that M. marianaensis is classified into Dermamoebida, and is a sister clade to other Mayorella species whose sequences are available. BLAST analysis revealed that M. marianaensis is most similar to Coronamoeba villafranca and Mayorella sp. JJP-2003, with sequence identities of 92.43% and 88.30%, respectively.</div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10005854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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