Protist最新文献 - Book学术

Genetic modification in dinoflagellate algae: Some successes, many failures 鞭毛藻的基因改造：一些成功，许多失败。

IF 2.1 3区生物学

Protist Pub Date : 2026-03-01 Epub Date: 2026-01-20 DOI: 10.1016/j.protis.2026.126148

Lucy Gavin, Catherine E. Evans, R. Ellen R. Nisbet

引用次数: 0

Experimental evidence of ciliate dispersal and successional patterns via endozoochory in temporary aquatic systems 临时水生系统中纤毛虫通过内胆扩散和演替模式的实验证据

IF 2.1 3区生物学

Protist Pub Date : 2026-03-01 Epub Date: 2025-11-30 DOI: 10.1016/j.protis.2025.126136

Crislaine Cochak , Andy J. Green , Marta I. Sánchez , Alfonso Pineda , Matheus Henrique de Oliveira de Matos , Luiz Felipe Machado Velho

{"title":"Experimental evidence of ciliate dispersal and successional patterns via endozoochory in temporary aquatic systems","authors":"Crislaine Cochak , Andy J. Green , Marta I. Sánchez , Alfonso Pineda , Matheus Henrique de Oliveira de Matos , Luiz Felipe Machado Velho","doi":"10.1016/j.protis.2025.126136","DOIUrl":"10.1016/j.protis.2025.126136","url":null,"abstract":"<div><div>Temporary aquatic ecosystems in Mediterranean regions rely on passive dispersal to maintain connectivity among isolated habitats. In Doñana National Park, Spain, aquatic birds enhance this connectivity by dispersing microbial propagules via endozoochory. We tested whether ciliate dispersal through bird feces facilitates community assembly and ecological succession in disconnected aquatic systems. Bird feces were incubated in aquatic microcosms for 42 days, and 39 ciliate morphospecies emerged. Species richness and abundance increased steadily, peaking between days 21 and 35. Functional succession started with small filter-feeders, followed by predators and mixotrophs. Despite taxonomic changes, ciliate body size remained stable, suggesting dispersal success depended more on cyst resistance than on morphological traits. The emergence of structured, functionally diverse communities confirms that waterbirds not only transport viable propagules but also actively enhance microbial connectivity and drive successional dynamics in temporary wetlands. These results reinforce endozoochory as a key mechanism shaping microbial metacommunities and supporting biodiversity in fragmented aquatic landscapes. Given that species were identified <em>in vivo</em> observation without molecular or staining confirmation, our results should be interpreted as community-level patterns rather than precise species-specific dynamics.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"180 ","pages":"Article 126136"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145665548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear localization signal in Leishmania spp.: Implications for parasite physiology and host nucleus hijacking 利什曼原虫核定位信号：寄生虫生理学和宿主核劫持的意义

IF 2.1 3区生物学

Protist Pub Date : 2026-03-01 Epub Date: 2026-01-08 DOI: 10.1016/j.protis.2026.126145

Lara da Mata Aredes Riguetti , Carlos Roberto Alves , Franklin Souza da Silva

{"title":"Nuclear localization signal in Leishmania spp.: Implications for parasite physiology and host nucleus hijacking","authors":"Lara da Mata Aredes Riguetti , Carlos Roberto Alves , Franklin Souza da Silva","doi":"10.1016/j.protis.2026.126145","DOIUrl":"10.1016/j.protis.2026.126145","url":null,"abstract":"<div><div>Protein transport between the cytoplasm and the nucleus is a fundamental process for the survival and proper functioning of all eukaryotic cells. This transport is regulated by adaptor proteins that recognize nuclear localization signals (NLSs) present in essential nuclear proteins. The data compiled in this review provide an overview of the classification and diversity of NLSs across various organisms. As in all eukaryotes, NLS-mediated transport is essential for <em>Leishmania</em> spp. For this parasite, there is evidence that NLS-mediated transport is essential for its physiology, allowing key proteins to perform crucial functions in maintaining intrinsic nuclear processes. Beyond classical nuclear proteins, this review indicates that some <em>Leishmania</em> proteases also contain NLSs, suggesting that these enzymes may have multifaceted roles: acting within the parasite itself and functioning as a strategy by the parasite to modulate the nuclear dynamics of the host cell. NLS-tagged metalloproteases of the GP63 family are known to interact with host nuclear components and inactivate critical transcription factors, thereby modulating cellular responses and promoting parasite survival. This function persists during the parasite's differentiation from the promastigote to the amastigote form, highlighting a continuous and sophisticated survival strategy. However, this mechanism still requires experimental confirmation. A deeper understanding of these processes offers promising perspectives for the development of novel therapeutic approaches against leishmaniasis.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"180 ","pages":"Article 126145"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The draft genome of the calcareous foraminifera Ammonia Veneta (rotaliida, globothalamea, foraminifera) 石灰质有孔虫氨Veneta（轮虫目，球藻目，有孔虫目）的基因组草图。

IF 2.1 3区生物学

Protist Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.protis.2025.126144

Yoshiyuki Ishitani , Shunichi Ishii , Yurika Ujiié

{"title":"The draft genome of the calcareous foraminifera Ammonia Veneta (rotaliida, globothalamea, foraminifera)","authors":"Yoshiyuki Ishitani , Shunichi Ishii , Yurika Ujiié","doi":"10.1016/j.protis.2025.126144","DOIUrl":"10.1016/j.protis.2025.126144","url":null,"abstract":"<div><div>Foraminifera are abundant and widely spread protists that contribute to global biogeochemical cycles and are used as bioindicators of polluted environments. However, their genomic basis remains poorly understood due to technical difficulties such as high genome complexity, microbial contamination, and the lack of axenic cultures. Here, we present the first draft genome of an axenic strain of a benthic foraminifer, <em>Ammonia veneta</em> WYF01. The 433 Mb genome assembly exhibits high completeness (80 % BUSCO score) and reveals a complex genomic landscape with extensive repeat content and 350,650 predicted gene models. Comparative genomic analysis with other rhizarians highlights conserved gene families related to pseudopodial motility and some osmoregulation, and substrate adhesion as species-specific expansions in <em>A. veneta</em>. Notably, we observed remarkable diversification of <em>glutathione S-transferase</em> genes, involved in detoxification of reactive oxygen species and xenobiotics, suggesting a genomic basis for this species' tolerance to polluted environments. Our findings provide valuable insights into the molecular adaptations of benthic Foraminifera and establish <em>A. veneta</em> as a genomic model for future evolutionary and environmental studies.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"180 ","pages":"Article 126144"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracing the Glow: Rethinking Luciferin Biosynthesis in Dinoflagellates 追踪发光：重新思考荧光素在鞭毛藻中的生物合成。

IF 2.1 3区生物学

Protist Pub Date : 2026-03-01 Epub Date: 2026-01-12 DOI: 10.1016/j.protis.2026.126146

James F.E. Vanstone , Jan Janouškovec

{"title":"Tracing the Glow: Rethinking Luciferin Biosynthesis in Dinoflagellates","authors":"James F.E. Vanstone , Jan Janouškovec","doi":"10.1016/j.protis.2026.126146","DOIUrl":"10.1016/j.protis.2026.126146","url":null,"abstract":"<div><div>Bioluminescence is a striking feature of many dinoflagellates, yet the origin of the luciferin substrate that underlies light emission remains unresolved. Previous studies have noted that luciferin is structurally similar to chlorophyll and related catabolites, but its presence in heterotrophs is puzzling, as such organisms have no need to produce chlorophyll. Hypothetical and sometimes conflicting views on several luciferin biosynthesis reactions have been proposed, but a conclusive template for the pathway is missing. Here, we integrate existing evidence into a model for luciferin biosynthesis based on three testable hypotheses. First, we posit that phototrophic and heterotrophic dinoflagellates are capable of <em>de novo</em> luciferin synthesis through a plastid-derived pathway. Secondly, we surmise that luciferin is derived from a specific pathway for chlorophyll degradation that includes the structurally similar pyropheophorbide <em>a</em>. Finally, we revisit the role of P630 as a likely biosynthetic precursor rather than a mere oxidation artefact. We then outline experimental strategies to test these hypotheses within the broader framework of tetrapyrrole metabolism. Resolving luciferin biosynthesis will elucidate not only the biochemical, spatial, and regulatory underpinning of bioluminescence but also illuminate its evolutionary origins and how ancestral metabolic pathways can be retooled for novel cellular functions.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"180 ","pages":"Article 126146"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Notable morphologies, molecular phylogenetics and taxonomy of the dinophyte Borghiella tenuissima (Borghiellaceae, Phytodiniales)” [Protist 179 (2025) 126132] 《细刺波氏菌（波氏菌科，植物didiiales）的显著形态、分子系统发育和分类》[原生生物179(2025)126132]的勘误。

IF 2.1 3区生物学

Protist Pub Date : 2026-03-01 Epub Date: 2026-01-18 DOI: 10.1016/j.protis.2026.126147

Büşra Şahin , Anna Müller , Paweł M. Owsianny , Corinna Romeikat , Danail P. Krastev , Juliana Chacón , Marc Gottschling

引用次数: 0

Environmental regulation of ciliates in corals: A secondary pathogen in coral disease 珊瑚中纤毛虫的环境调节：珊瑚病的次级病原体

IF 2.1 3区生物学

Protist Pub Date : 2025-12-01 Epub Date: 2025-11-23 DOI: 10.1016/j.protis.2025.126135

Lawrance Irudayarajan , Chinnarajan Ravindran , Haritha Parekkattumalayil Raveendran

{"title":"Environmental regulation of ciliates in corals: A secondary pathogen in coral disease","authors":"Lawrance Irudayarajan , Chinnarajan Ravindran , Haritha Parekkattumalayil Raveendran","doi":"10.1016/j.protis.2025.126135","DOIUrl":"10.1016/j.protis.2025.126135","url":null,"abstract":"<div><div>Ciliate invasion in corals is an emerging concern, yet the mechanisms behind their role in disease progression remain unclear. In this study, ciliates were isolated from pigmented lesions of <em>Porites lutea</em> and <em>Acropora muricata</em> and examined for their possible mode of interactions with coral tissues and bacteria. Histopathology revealed active bacterial feeding by ciliates along lesion margins, highlighting their potential role in worsening tissue damage. Microcosm experiments, designed to mimic seasonal conditions at the sampling site, showed that ciliate growth was most favorable during pre-monsoon conditions (32 °C, 36 ‰ salinity), while nutrient-rich post-monsoon environments (25 °C, 32 ‰) also supported proliferation. Importantly, maximum bacterial feeding occurred under pre-monsoon conditions, suggesting that elevated temperatures and mucus release from thermally stressed corals provide ideal resources for ciliates. This feeding may further extend to host-damaged tissue containing <em>Symbiodiniaceae</em>, disrupting coral recovery processes and increasing risks to reef biodiversity. These findings emphasize the importance of environmental factors and microbial interactions in shaping ciliate behaviour and highlight their role as secondary pathogens in coral disease dynamics.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"179 ","pages":"Article 126135"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomy and phylogeny of Phialina shanghaiensis sp. nov. and Lacrymaria filiformis (Maskell, 1886) Foissner, 1983 (Ciliophora, Litostomatea, Haptoria) from Changjiang Estuary, China 长江口海燕（philalina shanghaiensis sp. 11 .）和泪maria filiformis （Maskell, 1886）的分类和系统发育。Foissner, 1983 （Ciliophora, Litostomatea, Haptoria）

IF 2.1 3区生物学

Protist Pub Date : 2025-12-01 Epub Date: 2025-09-27 DOI: 10.1016/j.protis.2025.126131

Wei Xu , Jin Tang , Yu Zhao , Jiamei Jiang , Hongbo Pan

{"title":"Taxonomy and phylogeny of Phialina shanghaiensis sp. nov. and Lacrymaria filiformis (Maskell, 1886) Foissner, 1983 (Ciliophora, Litostomatea, Haptoria) from Changjiang Estuary, China","authors":"Wei Xu , Jin Tang , Yu Zhao , Jiamei Jiang , Hongbo Pan","doi":"10.1016/j.protis.2025.126131","DOIUrl":"10.1016/j.protis.2025.126131","url":null,"abstract":"<div><div><em>Lacrymaria</em> and <em>Phialina</em> are two closely related genera within Haptoria, yet the morphology and taxonomic affiliations of most species remain enigmatic. In the present study, we isolated two lacrymariid species, <em>Phialina shanghaiensis</em> sp. nov. and <em>Lacrymaria filiformis</em> (<span><span>Maskell, 1886</span></span>) <span><span>Foissner, 1983</span></span> from the Nanhui Wetland, Shanghai, China. Their morphology and phylogeny were investigated using live observation, protargol staining, and SSU rRNA gene sequencing. <em>P. shanghaiensis</em> sp. nov., is primarily distinguished from its congeners by its body size (100–125 × 20–30 μm in vivo), and possessing 12–15 somatic kineties, one macronucleus, and single terminally located contractile vacuole. The Chinese population of <em>L. filiformis</em> is also described in detail, which is characterized by 11–15 somatic kineties, one macronucleus, and a terminally located contractile vacuole. Phylogenetic analyses based on SSU rDNA sequences show that neither <em>Phialina</em> nor <em>Lacrymaria</em> is monophyletic, and the sequences of <em>P. salinarum</em> deposited in GenBank are likely from different <em>salinarum</em>-like species.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"179 ","pages":"Article 126131"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ceratium rhomvoides Hickel (Dinophyceae): Morphology, taxonomic implications, and expanded distribution 菱形角蜂（恐龙科）：形态、分类学意义及扩展分布

IF 2.1 3区生物学

Protist Pub Date : 2025-12-01 Epub Date: 2025-11-02 DOI: 10.1016/j.protis.2025.126133

Daniël Erasmus , Andrés Boltovskoy , Sanet Janse van Vuuren , Anatoliy Levanets

引用次数: 0

Diversity of Kryptoperidinium (Peridiniales, Dinophyceae): Morphological description and molecular phylogenetics of Kryptoperidinium secundum sp. nov. 潜甲的多样性：潜甲科潜甲科潜甲的形态描述和分子系统发育。

IF 2.1 3区生物学

Protist Pub Date : 2025-12-01 Epub Date: 2025-08-28 DOI: 10.1016/j.protis.2025.126120

Urban Tillmann , Marc Gottschling , Stephan Wietkamp , Ilka Peeken , Jennifer Wolny , Norico Yamada

{"title":"Diversity of Kryptoperidinium (Peridiniales, Dinophyceae): Morphological description and molecular phylogenetics of Kryptoperidinium secundum sp. nov.","authors":"Urban Tillmann , Marc Gottschling , Stephan Wietkamp , Ilka Peeken , Jennifer Wolny , Norico Yamada","doi":"10.1016/j.protis.2025.126120","DOIUrl":"10.1016/j.protis.2025.126120","url":null,"abstract":"<div><div><em>Kryptoperidinium</em> belongs to a group of dinophytes hosting a diatom as an endosymbiont and is currently considered to comprise a single, putatively bloom-forming and harmful species only. Molecular phylogenetics indicate the existence of a second distinct lineage and therefore species new to science, which we here formally describe as <em>Kryptoperidinium secundum</em> sp. nov. We studied living and fixed material of unialgal strains in detail using light and electron microscopy and gained DNA sequences of the rRNA complex (hosts and endosymbionts, from which also <em>psb</em>A and <em>rbc</em>L sequence data were obtained). In a molecular phylogeny of the Bacillariophyceae, the endosymbionts of <em>K</em>. <em>secundum</em> have a divergent position from those of <em>Kryptoperidinium triquetrum</em> (=<em>K. foliaceum</em>) and show (once more) a close relationship to free-living diatoms. The cells of <em>K. secundum</em> were strongly dorso-ventrally compressed and exhibited the thecal plate formula po, X, 4′, 2a, 7′′, 5C, 6(?)S, 5′′′, 2′′′′. The distalmost precingular plate was consistently rectangular in shape and relatively broad, and this is the key diagnostic trait to distinguish <em>K. secundum</em> from the known <em>K. triquetrum</em>, which has a characteristically L-shaped plate with a thin and elongated base. The two species are clearly divergent in molecular phylogenetics (exhibiting long branches) and constitute a monophyletic group together with <em>Dinothrix</em> sharing the same thecal plate formula. The diatom phylogeny favours an evolutionary scenario of repeated plastid capture rather than strict co-divergence between dinophyte hosts and their endosymbionts.</div></div>","PeriodicalId":20781,"journal":{"name":"Protist","volume":"179 ","pages":"Article 126120"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0