Flagellar proteomic analysis of the brown alga Mutimo cylindricus revealed a novel calcium-binding protein abundantly localized in the anterior flagellum

IF 1.9 3区 生物学 Q4 MICROBIOLOGY
Nana Kinoshita-Terauchi , Kogiku Shiba , Makoto Terauchi , Hideki Noguchi , Kazuo Inaba
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引用次数: 0

Abstract

Mutimo cylindricus gametes have two flagella with different structures : an anterior and a posterior flagellum. Their flagellar waveforms are regulated by calcium ions through various mechanisms, however the factors involved in this regulation remain largely unknown To elucidate the molecular basis underlying the difference between the two flagella, we performed a flagellar proteomic analysis of male M. cylindricus gametes. We identified 848 proteins shared with Ectocarpus siliculosus, including 28 calcium-binding proteins. Among the EF-hand proteins, a 111 kDa protein showed predominant localization along the anterior flagellum. Immunogold localization suggested that this protein is associated with outer doublet microtubules. This is the first report to show heterogeneous localization of a calcium-binding protein between two flagellar axonemes and suggests that calcium-binding proteins are involved in the specific regulation of the anterior flagellum.
褐藻 Mutimo cylindricus 的鞭毛蛋白质组分析发现了一种新型钙结合蛋白,该蛋白大量定位于前鞭毛中
圆柱木配子有两种不同结构的鞭毛:前鞭毛和后鞭毛。为了阐明两种鞭毛差异的分子基础,我们对雄性圆筒木配子进行了鞭毛蛋白质组分析。我们发现了848个与硅外皮藻共有的蛋白质,其中包括28个钙结合蛋白。在EF-手蛋白中,一个111 kDa的蛋白主要沿前鞭毛定位。免疫金定位表明,该蛋白与外双微管有关。这是首次报道钙结合蛋白在两个鞭毛轴丝之间的异质性定位,表明钙结合蛋白参与了前鞭毛的特异性调控。
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来源期刊
Protist
Protist 生物-微生物学
CiteScore
3.60
自引率
4.00%
发文量
43
审稿时长
18.7 weeks
期刊介绍: Protist is the international forum for reporting substantial and novel findings in any area of research on protists. The criteria for acceptance of manuscripts are scientific excellence, significance, and interest for a broad readership. Suitable subject areas include: molecular, cell and developmental biology, biochemistry, systematics and phylogeny, and ecology of protists. Both autotrophic and heterotrophic protists as well as parasites are covered. The journal publishes original papers, short historical perspectives and includes a news and views section.
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