Flagellar proteomic analysis of the brown alga Mutimo cylindricus revealed a novel calcium-binding protein abundantly localized in the anterior flagellum
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引用次数: 0
Abstract
Mutimo cylindricus gametes have two flagella with different structures : an anterior and a posterior flagellum. Their flagellar waveforms are regulated by calcium ions through various mechanisms, however the factors involved in this regulation remain largely unknown To elucidate the molecular basis underlying the difference between the two flagella, we performed a flagellar proteomic analysis of male M. cylindricus gametes. We identified 848 proteins shared with Ectocarpus siliculosus, including 28 calcium-binding proteins. Among the EF-hand proteins, a 111 kDa protein showed predominant localization along the anterior flagellum. Immunogold localization suggested that this protein is associated with outer doublet microtubules. This is the first report to show heterogeneous localization of a calcium-binding protein between two flagellar axonemes and suggests that calcium-binding proteins are involved in the specific regulation of the anterior flagellum.
期刊介绍:
Protist is the international forum for reporting substantial and novel findings in any area of research on protists. The criteria for acceptance of manuscripts are scientific excellence, significance, and interest for a broad readership. Suitable subject areas include: molecular, cell and developmental biology, biochemistry, systematics and phylogeny, and ecology of protists. Both autotrophic and heterotrophic protists as well as parasites are covered. The journal publishes original papers, short historical perspectives and includes a news and views section.