Proceedings of the Japan Academy. Series B, Physical and Biological Sciences最新文献_第4页

Conformational dynamics and molecular interactions of natural products: unveiling functional structures in biological membranes. 天然产物的构象动力学和分子相互作用：揭示生物膜的功能结构。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-05-09 Epub Date: 2025-03-31 DOI: 10.2183/pjab.101.016

Michio Murata, Masayuki Satake, Nobuaki Matsumori

引用次数: 0

Mechanism of puberulic acid contamination in red yeast rice tablets that caused a serious food poisoning outbreak in Japan. 红曲米片剂中微嫩酸污染导致日本严重食物中毒的机理。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-05-09 Epub Date: 2025-03-31 DOI: 10.2183/pjab.101.017

Tomoya Yoshinari, Maiko Watanabe, Wataru Aoki, Seiji Tanaka, Naoko Masumoto, Michiho Ito, Takahiro Ohnishi

{"title":"Mechanism of puberulic acid contamination in red yeast rice tablets that caused a serious food poisoning outbreak in Japan.","authors":"Tomoya Yoshinari, Maiko Watanabe, Wataru Aoki, Seiji Tanaka, Naoko Masumoto, Michiho Ito, Takahiro Ohnishi","doi":"10.2183/pjab.101.017","DOIUrl":"10.2183/pjab.101.017","url":null,"abstract":"In Japan, serious food poisoning among individuals who took supplement tablets for lowering plasma cholesterol levels have been publicized since late March 2024. The tablets were prepared from red yeast rice (RYR), a product of Monascus pilosus. Puberulic acid (PA) was detected as an unexpected compound in tablets that caused food poisoning. We conducted an on-site investigation at the RYR production factory to determine the cause of PA contamination of the tablets. Fungi capable of producing PA were detected in wipe samples from the factory and were identified as Penicillium adametzioides. To understand the route through which P. adametzioides contaminated RYR and produced PA, coculture experiments with M. pilosus and P. adametzioides were performed. P. adametzioides grew on rice covered with M. pilosus and produced PA. These results suggest that PA-producing P. adametzioides inhabited the RYR production factory and accidently contaminated the culture of M. pilosus. Consequently, RYR tablets contaminated with PA were manufactured and caused the food poisoning outbreak.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"302-316"},"PeriodicalIF":4.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12332415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis. 一种耐热高活性真菌GH3 β-葡萄糖苷酶，由随机诱变和饱和诱变产生。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-03-11 Epub Date: 2025-02-20 DOI: 10.2183/pjab.101.011

Chiaki Matsuzaki, Masafumi Hidaka, Yukari Nakashima, Yuji Honda, Takashi Koyanagi, Kazuhiko Ishikawa, Toshihiko Katoh, Takane Katayama, Hidehiko Kumagai

{"title":"A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis.","authors":"Chiaki Matsuzaki, Masafumi Hidaka, Yukari Nakashima, Yuji Honda, Takashi Koyanagi, Kazuhiko Ishikawa, Toshihiko Katoh, Takane Katayama, Hidehiko Kumagai","doi":"10.2183/pjab.101.011","DOIUrl":"10.2183/pjab.101.011","url":null,"abstract":"Enhancing the thermostability of cellulose-degrading enzymes is pivotal for establishing an efficient bioconversion system from cellulosic materials to value-added compounds. Here, by introducing random and saturation mutagenesis into the Thermoascus aurantiacus β-glucosidase gene, we generated a hyperthermostable mutant with five amino acid substitutions. Analysis of temperature-induced unfolding revealed the involvement of each replacement in the increased Tm value. Structural analysis showed that all replacements are located at the periphery of the catalytic pocket. D433N replacement, which had a pronounced thermostabilizing effect (ΔTm ＝ 4.5°C), introduced an additional hydrogen bond with a backbone carbonyl oxygen in a long loop structure. The mutant enzyme expressed in Kluyveromyces marxianus exhibited a Tm of 82°C and hydrolyzed cellobiose with kcat and Km values of 200 s－1 and 1.8 mM, respectively. When combined with a thermostable endoglucanase, the mutant enzyme released 20％ more glucose than wild-type enzyme from cellulosic material. The mutant enzyme is therefore a noteworthy addition to the existing repertoire of thermostable β-glucosidases.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"177-195"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thrombomodulin: A key regulator of intravascular blood coagulation, fibrinolysis, and inflammation, and a treatment for disseminated intravascular coagulation. 凝血调节素-血管内凝血和炎症的关键调节因子，以及弥散性血管内凝血的治疗方法。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-02-10 Epub Date: 2024-12-18 DOI: 10.2183/pjab.101.006

Koji Suzuki

{"title":"Thrombomodulin: A key regulator of intravascular blood coagulation, fibrinolysis, and inflammation, and a treatment for disseminated intravascular coagulation.","authors":"Koji Suzuki","doi":"10.2183/pjab.101.006","DOIUrl":"10.2183/pjab.101.006","url":null,"abstract":"Thrombomodulin (TM) is an important regulator of intravascular blood coagulation, fibrinolysis, and inflammation. TM inhibits the procoagulant and proinflammatory activities of thrombin and promotes the thrombin-induced activation of protein C (PC) bound to the endothelial PC receptor (EPCR). Activated PC (APC) inactivates coagulation factors Va and VIIIa, thereby inhibiting blood clotting. APC bound to EPCR exerts anti-inflammatory and cytoprotective effects on vascular endothelial cells. TM promotes the activation of thrombin-activatable fibrinolysis inhibitor, and also protects cells in blood vessels from inflammation caused by pathogen-associated and damaged cell-associated molecules. Excessive anticoagulant, fibrinolytic, anti-inflammatory, and tissue regenerative effects in the TM-PC pathway are controlled by PC inhibitor. A recombinant TM drug (TM), a soluble form of natural TM developed from the cloned human TM gene, has been evaluated for efficacy in many clinical trials and approved as a treatment for disseminated intravascular coagulation (DIC) caused by diseases such as sepsis, solid tumors, hematopoietic tumors, and trauma. It is currently widely used to treat DIC in Japan.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"75-97"},"PeriodicalIF":4.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance comparison of complementary relationship and inverse analysis methods for evapotranspiration estimation. 互补关系与逆分析方法在蒸散估算中的性能比较。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.028

Toshisuke Maruyama, Manabu Segawa, Hiroshi Takimoto

{"title":"Performance comparison of complementary relationship and inverse analysis methods for evapotranspiration estimation.","authors":"Toshisuke Maruyama, Manabu Segawa, Hiroshi Takimoto","doi":"10.2183/pjab.101.028","DOIUrl":"10.2183/pjab.101.028","url":null,"abstract":"Evapotranspiration is a critical factor that plays a pivotal role in irrigation and water resource planning. It is also a major influence with regard to global warming issues. Therefore, several studies have been performed on the estimation methods for evapotranspiration; however, the most prevalent method used over a long period to estimate evapotranspiration is the complementary relationship approach. Recently, this method was modified to improve its symmetry and accuracy. However, to achieve better performance, a different evapotranspiration estimation method using an inverse analysis of the Bowen ratio was proposed by us in an earlier study. In order to present a performance comparison of these different estimation methods, the reproducibility of three types of complementary relationship methods and inverse analysis methods was assessed in this study. Our study utilized data from FLUXNET2015 and evaluated the performance of the methods using regression analysis and root mean square error (RMSE) with data from 15 test sites, mainly located in the U.S.A., for a total period of 132 years. From the results, it was observed that the inverse analysis approach demonstrated a slightly better performance than the complementary relationship methods. This study provides a valuable direction for future research works on the estimation of evapotranspiration.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 8","pages":"485-509"},"PeriodicalIF":4.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145286745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical biology research in RIKEN NPDepo aimed at agricultural applications. 理化学研究所 NPDepo 以农业应用为目标的化学生物学研究。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.003

Hiroyuki Osada

{"title":"Chemical biology research in RIKEN NPDepo aimed at agricultural applications.","authors":"Hiroyuki Osada","doi":"10.2183/pjab.101.003","DOIUrl":"10.2183/pjab.101.003","url":null,"abstract":"This review outlines research on chemical biology using mainly microbial metabolites for agricultural applications. We established the RIKEN Natural Products Depository (NPDepo), housing many microbial metabolites, to support academic researchers who focus on drug discovery. We studied methods to stimulate secondary metabolism in microorganisms to collect various microbial products. The switch of secondary metabolism in microorganisms changes depending on the culture conditions. We discovered compounds that activate biosynthetic gene clusters in actinomycetes and filamentous fungi. Using these compounds, we succeeded in inducing the production of active compounds. Two approaches for screening bioactive compounds are described. One is phenotypic screening to explore antifungal compounds assisted by artificial intelligence (AI). AI can distinguish the morphological changes induced by antifungal compounds in filamentous fungi. The other is the chemical array method for detecting interactions between compounds and target proteins. Our chemical biology approach yielded many new compounds as fungicide candidates.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 1","pages":"8-31"},"PeriodicalIF":4.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pursuing the identity of a natural medicine licorice by Dr. Shoji Shibata that he firstly investigated half a century before. 寻找柴田正二博士在半个世纪前首次研究的天然药物甘草的身份。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.018

Michiho Ito

引用次数: 0

Multi-region processing during sleep for memory and cognition. 睡眠中记忆和认知的多区域加工。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.008

Salma E Said, Daisuke Miyamoto

{"title":"Multi-region processing during sleep for memory and cognition.","authors":"Salma E Said, Daisuke Miyamoto","doi":"10.2183/pjab.101.008","DOIUrl":"10.2183/pjab.101.008","url":null,"abstract":"Over the past decades, the understanding of sleep has evolved to be a fundamental physiological mechanism integral to the processing of different types of memory rather than just being a passive brain state. The cyclic sleep substates, namely, rapid eye movement (REM) sleep and non-REM (NREM) sleep, exhibit distinct yet complementary oscillatory patterns that form inter-regional networks between different brain regions crucial to learning, memory consolidation, and memory retrieval. Technical advancements in imaging and manipulation approaches have provided deeper understanding of memory formation processes on multi-scales including brain-wide, synaptic, and molecular levels. The present review provides a short background and outlines the current state of research and future perspectives in understanding the role of sleep and its substates in memory processing from both humans and rodents, with a focus on cross-regional brain communication, oscillation coupling, offline reactivations, and engram studies. Moreover, we briefly discuss how sleep contributes to other higher-order cognitive functions.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 3","pages":"107-128"},"PeriodicalIF":4.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common and distinct features of diverse macrophage populations in the central nervous system. 中枢神经系统中不同巨噬细胞群的共同和独特特征。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.013

Takahiro Masuda

引用次数: 0

Cellular senescence as a source of chronic microinflammation that promotes the aging process. 细胞衰老是促进衰老过程的慢性微炎症的来源。

IF 4.6 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.014

Makoto Nakanishi

{"title":"Cellular senescence as a source of chronic microinflammation that promotes the aging process.","authors":"Makoto Nakanishi","doi":"10.2183/pjab.101.014","DOIUrl":"10.2183/pjab.101.014","url":null,"abstract":"Why and how do we age? This physiological phenomenon that we all experience remains a great mystery, largely unexplained even in this age of scientific and technological progress. Aging is a significant risk factor for numerous diseases, including cancer. However, underlying mechanisms responsible for this association remain to be elucidated. Recent findings have elucidated the significance of the accumulation of senescent cells and other inflammatory cells in organs and tissues with age, and their deleterious effects, such as the induction of inflammation in the microenvironment, as underlying factors contributing to organ dysfunction and disease development. Cellular senescence is a cellular phenomenon characterized by a permanent cessation of cell proliferation and secretion of several proinflammatory cytokines (senescence associated secretory phenotypes). Notably, the elimination of senescent cells from aging individuals has been demonstrated to alleviate age-related organ and tissue dysfunction, as well as various geriatric diseases. This review summarizes the molecular mechanisms by which senescent cells are induced and contribute to age-related diseases, as well as the technologies that ameliorate them.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 4","pages":"224-237"},"PeriodicalIF":4.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0