Proceedings of the Japan Academy. Series B, Physical and Biological Sciences最新文献

Conformational dynamics and molecular interactions of natural products: unveiling functional structures in biological membranes. 天然产物的构象动力学和分子相互作用：揭示生物膜的功能结构。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-03-31 DOI: 10.2183/pjab.101.016

Michio Murata, Masayuki Satake, Nobuaki Matsumori

引用次数: 0

Mechanism of puberulic acid contamination in red yeast rice tablets that caused a serious food poisoning outbreak in Japan. 红曲米片剂中微嫩酸污染导致日本严重食物中毒的机理。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-03-31 DOI: 10.2183/pjab.101.017

Tomoya Yoshinari, Maiko Watanabe, Wataru Aoki, Seiji Tanaka, Naoko Masumoto, Michiho Ito, Takahiro Ohnishi

{"title":"Mechanism of puberulic acid contamination in red yeast rice tablets that caused a serious food poisoning outbreak in Japan.","authors":"Tomoya Yoshinari, Maiko Watanabe, Wataru Aoki, Seiji Tanaka, Naoko Masumoto, Michiho Ito, Takahiro Ohnishi","doi":"10.2183/pjab.101.017","DOIUrl":"https://doi.org/10.2183/pjab.101.017","url":null,"abstract":"In Japan, serious food poisoning among individuals who took supplement tablets for lowering plasma cholesterol levels have been publicized since late March 2024. The tablets were prepared from red yeast rice (RYR), a product of Monascus pilosus. Puberulic acid (PA) was detected as an unexpected compound in tablets that caused food poisoning. We conducted an on-site investigation at the RYR production factory to determine the cause of PA contamination of the tablets. Fungi capable of producing PA were detected in wipe samples from the factory and were identified as Penicillium adametzioides. To understand the route through which P. adametzioides contaminated RYR and produced PA, coculture experiments with M. pilosus and P. adametzioides were performed. P. adametzioides grew on rice covered with M. pilosus and produced PA. These results suggest that PA-producing P. adametzioides inhabited the RYR production factory and accidently contaminated the culture of M. pilosus. Consequently, RYR tablets contaminated with PA were manufactured and caused the food poisoning outbreak.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis. 一种耐热高活性真菌GH3 β-葡萄糖苷酶，由随机诱变和饱和诱变产生。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-03-11 Epub Date: 2025-02-20 DOI: 10.2183/pjab.101.011

Chiaki Matsuzaki, Masafumi Hidaka, Yukari Nakashima, Yuji Honda, Takashi Koyanagi, Kazuhiko Ishikawa, Toshihiko Katoh, Takane Katayama, Hidehiko Kumagai

{"title":"A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis.","authors":"Chiaki Matsuzaki, Masafumi Hidaka, Yukari Nakashima, Yuji Honda, Takashi Koyanagi, Kazuhiko Ishikawa, Toshihiko Katoh, Takane Katayama, Hidehiko Kumagai","doi":"10.2183/pjab.101.011","DOIUrl":"10.2183/pjab.101.011","url":null,"abstract":"Enhancing the thermostability of cellulose-degrading enzymes is pivotal for establishing an efficient bioconversion system from cellulosic materials to value-added compounds. Here, by introducing random and saturation mutagenesis into the Thermoascus aurantiacus β-glucosidase gene, we generated a hyperthermostable mutant with five amino acid substitutions. Analysis of temperature-induced unfolding revealed the involvement of each replacement in the increased Tm value. Structural analysis showed that all replacements are located at the periphery of the catalytic pocket. D433N replacement, which had a pronounced thermostabilizing effect (ΔTm ＝ 4.5°C), introduced an additional hydrogen bond with a backbone carbonyl oxygen in a long loop structure. The mutant enzyme expressed in Kluyveromyces marxianus exhibited a Tm of 82°C and hydrolyzed cellobiose with kcat and Km values of 200 s－1 and 1.8 mM, respectively. When combined with a thermostable endoglucanase, the mutant enzyme released 20％ more glucose than wild-type enzyme from cellulosic material. The mutant enzyme is therefore a noteworthy addition to the existing repertoire of thermostable β-glucosidases.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"177-195"},"PeriodicalIF":4.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thrombomodulin: A key regulator of intravascular blood coagulation, fibrinolysis, and inflammation, and a treatment for disseminated intravascular coagulation. 凝血调节素-血管内凝血和炎症的关键调节因子，以及弥散性血管内凝血的治疗方法。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-02-10 Epub Date: 2024-12-18 DOI: 10.2183/pjab.101.006

Koji Suzuki

{"title":"Thrombomodulin: A key regulator of intravascular blood coagulation, fibrinolysis, and inflammation, and a treatment for disseminated intravascular coagulation.","authors":"Koji Suzuki","doi":"10.2183/pjab.101.006","DOIUrl":"10.2183/pjab.101.006","url":null,"abstract":"Thrombomodulin (TM) is an important regulator of intravascular blood coagulation, fibrinolysis, and inflammation. TM inhibits the procoagulant and proinflammatory activities of thrombin and promotes the thrombin-induced activation of protein C (PC) bound to the endothelial PC receptor (EPCR). Activated PC (APC) inactivates coagulation factors Va and VIIIa, thereby inhibiting blood clotting. APC bound to EPCR exerts anti-inflammatory and cytoprotective effects on vascular endothelial cells. TM promotes the activation of thrombin-activatable fibrinolysis inhibitor, and also protects cells in blood vessels from inflammation caused by pathogen-associated and damaged cell-associated molecules. Excessive anticoagulant, fibrinolytic, anti-inflammatory, and tissue regenerative effects in the TM-PC pathway are controlled by PC inhibitor. A recombinant TM drug (TM), a soluble form of natural TM developed from the cloned human TM gene, has been evaluated for efficacy in many clinical trials and approved as a treatment for disseminated intravascular coagulation (DIC) caused by diseases such as sepsis, solid tumors, hematopoietic tumors, and trauma. It is currently widely used to treat DIC in Japan.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"75-97"},"PeriodicalIF":4.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical biology research in RIKEN NPDepo aimed at agricultural applications. 理化学研究所 NPDepo 以农业应用为目标的化学生物学研究。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.003

Hiroyuki Osada

{"title":"Chemical biology research in RIKEN NPDepo aimed at agricultural applications.","authors":"Hiroyuki Osada","doi":"10.2183/pjab.101.003","DOIUrl":"10.2183/pjab.101.003","url":null,"abstract":"This review outlines research on chemical biology using mainly microbial metabolites for agricultural applications. We established the RIKEN Natural Products Depository (NPDepo), housing many microbial metabolites, to support academic researchers who focus on drug discovery. We studied methods to stimulate secondary metabolism in microorganisms to collect various microbial products. The switch of secondary metabolism in microorganisms changes depending on the culture conditions. We discovered compounds that activate biosynthetic gene clusters in actinomycetes and filamentous fungi. Using these compounds, we succeeded in inducing the production of active compounds. Two approaches for screening bioactive compounds are described. One is phenotypic screening to explore antifungal compounds assisted by artificial intelligence (AI). AI can distinguish the morphological changes induced by antifungal compounds in filamentous fungi. The other is the chemical array method for detecting interactions between compounds and target proteins. Our chemical biology approach yielded many new compounds as fungicide candidates.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 1","pages":"8-31"},"PeriodicalIF":4.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of autophagosome formation. 自噬体的形成机制

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.005

Yuko Fujioka, Nobuo N. Noda

引用次数: 0

Multi-region processing during sleep for memory and cognition. 睡眠中记忆和认知的多区域加工。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.008

Salma E Said, Daisuke Miyamoto

引用次数: 0

Pathogenesis of type 2 diabetes in Japan and East Asian populations: Basic and clinical explorations. 日本和东亚人群2型糖尿病的发病机制：基础和临床探索。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.009

Yutaka Seino, Yuji Yamazaki

引用次数: 0

First English article of Yagi-Uda antenna. 八木田天线的第一篇英文文章。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.001

Qiang Chen

引用次数: 0

Peaks in weak lensing mass maps for cluster astrophysics and cosmology. 星系团天体物理学和宇宙学中弱透镜质量图的峰值。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI: 10.2183/pjab.101.010

Masamune Oguri, Satoshi Miyazaki

{"title":"Peaks in weak lensing mass maps for cluster astrophysics and cosmology.","authors":"Masamune Oguri, Satoshi Miyazaki","doi":"10.2183/pjab.101.010","DOIUrl":"https://doi.org/10.2183/pjab.101.010","url":null,"abstract":"Clusters of galaxies can be identified from the peaks in weak lensing aperture mass maps constructed from weak lensing shear catalogs. Such purely gravitational cluster selection differs considerably from traditional cluster selection based on the baryonic properties of clusters. In this review, we present the basics and applications of weak lensing shear-selected cluster samples. Detailed studies of the baryonic properties of shear-selected clusters shed new light on cluster astrophysics. The purely gravitational selection indicates that the selection function can be quantified more easily and robustly, which is crucial for deriving accurate cosmological constraints from the abundance of shear-selected clusters. Recent advances in shear-selected cluster studies are driven by the Subaru Hyper Suprime-Cam survey, in which more than 300 shear-selected clusters with a signal-to-noise ratio ＞ 5 were identified. It is argued that various systematic effects in cosmological analysis can be mitigated by carefully selecting the setup of the analysis, including the selection of kernel functions and the source galaxy sample.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 3","pages":"129-142"},"PeriodicalIF":4.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0