Preventive Nutrition and Food Science最新文献

Comparison of Methods for Evaluating Drivers of Liking for Yakju: Ideal Napping versus the Check-All-That-Apply Method. “理想午睡法”与“全检法”的比较。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.409

Jiyoon Jung, Han Sub Kwak, Sangsook Kim, Sung-Jin Park, Dongju Lee, Mohammed I Saleh, Youngseung Lee

{"title":"Comparison of Methods for Evaluating Drivers of Liking for Yakju: Ideal Napping versus the Check-All-That-Apply Method.","authors":"Jiyoon Jung, Han Sub Kwak, Sangsook Kim, Sung-Jin Park, Dongju Lee, Mohammed I Saleh, Youngseung Lee","doi":"10.3746/pnf.2025.30.4.409","DOIUrl":"10.3746/pnf.2025.30.4.409","url":null,"abstract":"Check-all-that-apply (CATA) and napping are cost- and time-efficient alternatives to conventional descriptive analysis for evaluating product sensory characteristics. This study introduces ideal napping as a novel sensory evaluation method that integrates the napping procedure with the concept of constructing an ideal product profile. Ideal napping was used to identify drivers of liking for 12 commercial yakjus (Korean rice wines). When comparing the product configurations generated by the multiple factor analysis between the two methods, a similar representation of product locations, including the ideal product, was observed. The ideal product was characterized by statements such as \"fruit-related,\" \"green plum-related,\" and \"sweetness,\" while the \"yeast-related\" attributes were far from ideal according to the results of both the methods. Drivers of liking or disliking were identified by partial least squares regression for these two methods and yielded similar results, revealing the main drivers of liking to be \"sweetness,\" \"sweet aroma,\" \"fruit flavor and aroma,\" \"green plum flavor,\" \"floral aroma,\" and \"carbonic mouthfeel,\" while the main drivers of disliking were \"acridity,\" \"bitterness,\" \"yeast flavor and aroma,\" and \"mushroom flavor and aroma.\" Ideal napping was able to provide additional insights into the consumer perception of ideal products with performance similar to CATA. The findings offer practical insights for liquor product development and reformulation. By identifying sensory attributes that influence consumer preferences, manufacturers can better align products with market expectations. Additionally, comparing ideal napping with CATA provides valuable guidance for improving consumer satisfaction with traditional beverages such as yakju.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"409-418"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Optimized Cysteine Protease-Aided Protein Hydrolysates of Soybean Tempeh Inhibit Angiotensin-Converting Enzyme Activity In Vitro. 两种优化的半胱氨酸蛋白酶辅助蛋白水解大豆豆豉体外抑制血管紧张素转换酶活性的研究。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.379

Sukrasno Sukrasno, Defri Rizaldy, Neng Fisheri Kurniati, Cut Raihanah, Arif Al Iman

{"title":"Two Optimized Cysteine Protease-Aided Protein Hydrolysates of Soybean Tempeh Inhibit Angiotensin-Converting Enzyme Activity In Vitro.","authors":"Sukrasno Sukrasno, Defri Rizaldy, Neng Fisheri Kurniati, Cut Raihanah, Arif Al Iman","doi":"10.3746/pnf.2025.30.4.379","DOIUrl":"10.3746/pnf.2025.30.4.379","url":null,"abstract":"Peptides produced from soybean tempeh that inhibit angiotensin-converting enzyme (ACE) provide a promising source of novel antihypertensive agents. This study utilized two cysteine proteases (papain and bromelain) to generate ACE inhibitory peptides from the protein hydrolysate of soybean tempeh. The trials were arranged using a Box-Behnken design to achieve optimal hydrolysis conditions. The optimal conditions, which were determined by response surface methodology optimization, were as follows: papain: pH 9, temperature 62.88°C, enzyme/substrate ratio 0.54%, and hydrolysis duration 14.94 h; bromelain: pH 9, temperature 60°C, enzyme/substrate ratio 1%, and hydrolysis duration 1 h. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography tandem mass spectrometry analysis, the protein hydrolysates displayed peptide bands within the 23－30 kDa range, comprising various peptides from monopeptides to nonapeptides. Moreover, these protein hydrolysates exhibited a more pronounced ACE inhibition than the crude protein extract, with the lowest IC50 value obtained from the protein hydrolysate with papain. These findings offer a conceptual basis for the development of food-derived ACE inhibitory peptides and the highly valued application of soybean tempeh.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"379-390"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Effects of an Eight-Week Resistance Training and Probiotic Supplementation on Bone Metabolism in Ovariectomized Rats. 8周抗阻训练和补充益生菌对去卵巢大鼠骨代谢的协同作用。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.323

Sogand Nasabeh Asl, Farhad Daryanoosh, Seyyed Shahram Shekarforoush, Nader Tanideh, Omid Koohi-Hosseinabadi, Arash Khorshidian, Amir Hossein Zarifkar

{"title":"Synergistic Effects of an Eight-Week Resistance Training and Probiotic Supplementation on Bone Metabolism in Ovariectomized Rats.","authors":"Sogand Nasabeh Asl, Farhad Daryanoosh, Seyyed Shahram Shekarforoush, Nader Tanideh, Omid Koohi-Hosseinabadi, Arash Khorshidian, Amir Hossein Zarifkar","doi":"10.3746/pnf.2025.30.4.323","DOIUrl":"10.3746/pnf.2025.30.4.323","url":null,"abstract":"Aging is one of the most influential factors in bone metabolism, leading to a universal decline in bone density in both men and women. This study aimed to investigate the effects of eight weeks of resistance training and probiotic supplementation on bone metabolism in ovariectomized rats. A total of 48 female Sprague-Dawley rats were randomly assigned to six groups: control, sham, ovariectomy, ovariectomy+probiotic supplementation, ovariectomy+resistance training, and ovariectomy+resistance training+probiotic supplementation. Seven days post-ovariectomy, the exercise protocol commenced, consisting of ladder climbing with weights attached to the rat's tail. Additionally, each rat received 1 mL of probiotic supplement daily for eight weeks. The results showed no significant difference in serum phosphorus levels among the groups [P=0.133, F(5, 42)=1.804]. However, significant differences were observed in alkaline phosphatase levels [P<0.001, F(5, 42)=43.05], calcium levels [P=0.016, F(5, 42)=3.186], and bone mechanical strength [P<0.001, F(5, 42)=18.136]. These findings suggest that after eight weeks of probiotic supplementation and resistance training exert synergistic positive effects on serum calcium and alkaline phosphatase levels, as well as bone strength.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"323-330"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physicochemical and Sensory Evaluation of Low-Fat Yogurt Treated with Alfalfa Seed Powder. 苜蓿籽粉处理低脂酸奶的理化及感官评价。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.349

Amin Sharifi, Vahid Ghasemzadeh-Mohammadi, Alireza Farsad-Naeimi

{"title":"Physicochemical and Sensory Evaluation of Low-Fat Yogurt Treated with Alfalfa Seed Powder.","authors":"Amin Sharifi, Vahid Ghasemzadeh-Mohammadi, Alireza Farsad-Naeimi","doi":"10.3746/pnf.2025.30.4.349","DOIUrl":"10.3746/pnf.2025.30.4.349","url":null,"abstract":"Recently, alfalfa (Medicago sativa) has been used to improve the nutritional quality of food products, including gluten-free cookies and biscuits. In the present study, the effects of the addition of alfalfa seed powder on the physicochemical, sensory, and antioxidant properties of low-fat yogurt (1.5%) were investigated. Different concentrations of alfalfa seed powder (Y0, control; Y1, 0.25%; Y2, 0.5%; Y3, 0.75%) were used to prepare yogurt samples, which were stored for 14 days at 4°C. Following enrichment, the acidity and viscosity of the samples increased. However, the pH and syneresis significantly decreased over time with increasing alfalfa seed powder concentrations (P<0.05). The inclusion of alfalfa significantly enhanced the antioxidant properties of samples. On the first day, Y3 showed the highest total phenolic content (27.35±0.25 mg GAE/g), 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (51.76%±0.68%), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) activity (83.65%±0.27%) among all samples (P<0.05). The addition of alfalfa seed powder also significantly improved the yogurt's lipid profile by increasing the levels of α-linolenic acid (6.19%±0.61% in Y3 vs. 0.55%±0.08% in Y0) and linoleic acid (5.88%±0.43% in Y3 vs. 3.59%±0.17% in Y0) and decreasing the levels of saturated fatty acids (P<0.05). During storage, the color parameters of Y3, including lightness and whiteness, significantly decreased, but its yellowness increased compared with that of Y0 (P<0.05). However, the sensory properties did not significantly change in any of the yogurt samples. These results suggest that the addition of alfalfa seed powder enhances the functional and nutritional value of yogurt, and it can be considered as a potential functional food ingredient.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"349-359"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Obesity Effects of Abeliophyllum distichum Extracts via the AMPK Signaling Pathway in 3T3-L1 Adipocytes. abelophyllum distichum提取物通过3T3-L1脂肪细胞AMPK信号通路的抗肥胖作用。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.360

Sun Ok Han, Geon Kim, Youn Young Shim, Dong-Sub Kim, Nak-Yun Sung, Eun Yeong Nho, Sang-Yun Park, Hye-Lin Kim, Young Jun Kim, Yunu Jung

{"title":"Anti-Obesity Effects of Abeliophyllum distichum Extracts via the AMPK Signaling Pathway in 3T3-L1 Adipocytes.","authors":"Sun Ok Han, Geon Kim, Youn Young Shim, Dong-Sub Kim, Nak-Yun Sung, Eun Yeong Nho, Sang-Yun Park, Hye-Lin Kim, Young Jun Kim, Yunu Jung","doi":"10.3746/pnf.2025.30.4.360","DOIUrl":"10.3746/pnf.2025.30.4.360","url":null,"abstract":"Obesity is a major public health concern because of its association with metabolic disorders (e.g., type 2 diabetes mellitus) and cardiovascular diseases. Natural compounds are increasingly being explored as safer alternatives to synthetic antiobesity drugs. In this study, the antiobesity effects of Abeliophyllum distichum extracts in 3T3-L1 adipocytes and their underlying mechanisms were investigated. Oil Red O staining was employed to assess the effects of A. distichum extracts on adipogenesis and lipid accumulation, and Western blot analysis and quantitative polymerase chain reaction were used to analyze the key molecular pathways were. Treatment with A. distichum extracts significantly inhibited lipid accumulation and suppressed the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, and sterol regulatory element-binding protein 1c, at the protein and mRNA levels. Furthermore, treatment with A. distichum extracts activated AMP-activated protein kinase (AMPK) and enhanced the phosphorylation of acetyl-CoA carboxylase (ACC), which are key regulators of cellular energy metabolism, while reducing the total ACC expression. These findings indicate that A. distichum extracts exert antiobesity effects by modulating the AMPK signaling pathway and inhibiting adipogenesis. Given these significant bioactive properties, A. distichum extracts have promising application potential as a natural therapeutic agent for treating obesity.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"360-369"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Intervention in the Soaking Step during Tempeh Preparation to Modulate B Vitamin Contents: A Metabolomics Approach. 豆豉浸泡过程中微生物干预对B族维生素含量的调节：代谢组学方法。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.370

Rifqi Ahmad Riyanto, Eiichiro Fukusaki, Sastia Prama Putri

{"title":"Microbial Intervention in the Soaking Step during Tempeh Preparation to Modulate B Vitamin Contents: A Metabolomics Approach.","authors":"Rifqi Ahmad Riyanto, Eiichiro Fukusaki, Sastia Prama Putri","doi":"10.3746/pnf.2025.30.4.370","DOIUrl":"10.3746/pnf.2025.30.4.370","url":null,"abstract":"B vitamins are integral in maintaining human health. Owing to its high B vitamin contents, tempeh serves as an excellent plant-based option to achieve an adequate intake of these nutrients through diet. In order to investigate the metabolomic profile of tempeh and modulate the contents of vitamins B2 and B3 in it, we employed microbial interventions using two species of lactic acid bacteria (LAB) and various inoculum sizes in the soaking step during tempeh production. We thoroughly analyzed the tempeh samples using a wide-targeted metabolomics approach via gas chromatography-mass spectrometry. A total of 86 metabolites, including sugars, amino acids, fatty acids, and organic acids, were annotated in all the samples. Principal component analysis revealed that the two LAB species led to alterations in the tempeh metabolome with sugars and amino acids separating it. Additionally, variations in inoculum size influenced the metabolomic profile of tempeh as well. Furthermore, the highest B vitamin concentrations in the tempeh samples were observed when 5% v/v Lactiplantibacillus plantarum was incorporated during the soaking process. Specifically 5.312±0.315 mg of vitamin B2 per 100 g of food and 10.334±0.138 mg of vitamin B3 per 100 g of food were detected, which corresponded to a 1.22-fold and 1.08-fold increase compared to the contents observed in tempeh obtained from water-soaked soybean. These findings will assist in advancing tempeh production and will also contribute to innovation in the broader field of fermented food processing.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"370-378"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dairy-Derived Bioactive Peptides and the Risk of Prostate Cancer among Iranian Men: A Case-Control Study. 乳源性生物活性肽与伊朗男性前列腺癌风险：一项病例对照研究

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.305

Karim Parastouei, Masoumeh Jabbari, Meisam Barati, Ali Najafi, Maryam Aryafar, Reihaneh Zeinalian, Bahram Rashidkhani

{"title":"Dairy-Derived Bioactive Peptides and the Risk of Prostate Cancer among Iranian Men: A Case-Control Study.","authors":"Karim Parastouei, Masoumeh Jabbari, Meisam Barati, Ali Najafi, Maryam Aryafar, Reihaneh Zeinalian, Bahram Rashidkhani","doi":"10.3746/pnf.2025.30.4.305","DOIUrl":"10.3746/pnf.2025.30.4.305","url":null,"abstract":"This case-control study aimed to assess the association of dairy-derived digestion-resistant bioactive peptides (BPs) with the risk of prostate cancer (PCa) in a cohort of 50 men with PCa and 99 controls. Dietary, demographic, and anthropometric factors of all participants were assessed. Various types of dairy-derived BPs were determined by an in silico method. High intake of total digestion-resistant BPs, low molecular weight peptides, high molecular weight peptides, phosphorylated residues, disulfide bonds, and antioxidant peptides was not significantly associated with the risk of PCa in the adjusted models (P>0.05), while low-fat [odds ratio (OR): 0.19, 95% confidence intervals (CI): 0.05－0.66] and total dairy intake (OR: 0.23, 95% CI: 0.06－0.79) were inversely associated with PCa risk. To the best of our knowledge, this study is the first to report an association between dairy-derived BPs and the risk of PCa and an inverse association with consumption of dairy products in Iranian men likely due to the low intake of this population. Notably, the BP content of dairy products was not significantly associated with PCa risk.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"305-311"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Risk Factors for Coronary Heart Disease in Middle-Aged Men Using the Framingham Risk Score: Based on the Korea National Health and Nutrition Examination Survey (2014－2015). 基于韩国国民健康与营养调查（2014-2015）的中年男性冠心病危险因素Framingham风险评分分析

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.295

Yeong Jeon, Kyoung Yun Kim, Jung-Mi Yun

{"title":"Analysis of Risk Factors for Coronary Heart Disease in Middle-Aged Men Using the Framingham Risk Score: Based on the Korea National Health and Nutrition Examination Survey (2014－2015).","authors":"Yeong Jeon, Kyoung Yun Kim, Jung-Mi Yun","doi":"10.3746/pnf.2025.30.4.295","DOIUrl":"10.3746/pnf.2025.30.4.295","url":null,"abstract":"The Framingham risk score (FRS) is used to assess an individual's risk of developing coronary heart disease (CHD) within 10 years by evaluating CHD risk factors. Despite the increasing mortality rate from CHD, there are limited studies examining the association between the atherogenic coefficient (AC), atherogenic index of plasma (AIP), dietary intake (assessed using a food frequency questionnaire), and FRS-CHD in Korea. The FRS is based on the following coronary risk factors: age, sex, total cholesterol, high-density lipoprotein cholesterol, smoking habits, and systolic blood pressure. The present study aimed to examine the factors that increase the risk of developing CHD in middle-aged men using the FRS based on the 2014－2015 Korea National Health and Nutrition Examination Survey (KNHANES). Data from 1,478 middle-aged men who participated in the 2014－2015 KNHANES were used. As the AC [odds ratio (OR), 6.06; 95% confidence interval (CI), 4.38－8.38; P<0.001] and AIP (OR, 3.93; 95% CI, 1.93－8.01; P<0.001) increased by 1 unit, the risk of developing CHD was significantly higher in the high-risk group than in the low-risk group after adjusting for potential confounding factors. In the high-risk group, the CHD risk decreased with an increase in egg intake per serving (OR, 0.84; 95% CI, 0.75－0.94; P<0.01). The AC, AIP, and egg intake were potent determinants of the high-risk group compared with the low-risk group. Blood cholesterol levels and diet control can decrease the CHD risk in the next 10 years.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"295-304"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Effects of a Combination of Scutellaria baicalensis and Nelumbo nuciferaon Inflammation and Hyperuricemia. 黄芩联合莲子对炎症和高尿酸血症的双重作用。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.340

Jung Up Park, Se Jin Jo, Yue Gong, Rui Jiang, Jungu Lee, Jae-Woong Park, Jungki Kwak, Young Ran Kim

{"title":"Dual Effects of a Combination of Scutellaria baicalensis and Nelumbo nuciferaon Inflammation and Hyperuricemia.","authors":"Jung Up Park, Se Jin Jo, Yue Gong, Rui Jiang, Jungu Lee, Jae-Woong Park, Jungki Kwak, Young Ran Kim","doi":"10.3746/pnf.2025.30.4.340","DOIUrl":"10.3746/pnf.2025.30.4.340","url":null,"abstract":"This study developed an effective combination of Scutellaria baicalensis and Nelumbo nucifera (SNE) and evaluated its anti-inflammatory and anti-hyperuricemic effects under in vivo conditions. First, the effect of SNE was tested on in vitro xanthine oxidase (XOD) activity. To investigate the anti-inflammatory effect of SNE, nitric oxide (NO) production was detected by Griess assay, and proinflammatory cytokines were measured by enzyme-linked immunosorbent assay in RAW264.7 cells. Next, we examined the effect of SNE on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) production using Western blot analysis. NF-κB transcriptional activity was measured using the NF-κB-SEAP reporter plasmid. To confirm the anti-hyperuricemia effect of SNE, potassium oxonate (PO)-induced mouse model was established, and the serum was used to measure glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, blood urea nitrogen, creatinine, and XOD levels. Through extensive screening for a herbal medicine library, we found that SNE exhibited a potent inhibitory effect on XOD activity. In addition, SNE remarkably inhibited the production of proinflammatory cytokines [e.g., NO, interleukin (IL)-1β, and IL-6] in lipopolysaccharide-induced RAW264.7 cells and suppressed the promoter activity of NF-κB. SNE also inhibited iNOS and COX-2 expression. Finally, SNE showed anti-hyperuricemic effects in a mouse model of PO-induced hyperuricemia and did not exhibit any toxicity to liver and kidney functions. SNE, a water extract from a mixture of S. baicalensis and N. nucifera at a ratio of 1:1 (w/w), is a good herbal combination that possesses dual therapeutic activities on inflammation and hyperuricemia.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"340-348"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antitumor Effects of Metformin in Squamous Cell Carcinoma under Leptin Treatment Conditions. 瘦素治疗下二甲双胍对鳞状细胞癌的抗肿瘤作用。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2025-08-31 DOI: 10.3746/pnf.2025.30.4.312

Sujung Yeom, Danbi Jo, Seo Yoon Choi, Seo Yeon Ahn, Dong Hoon Lee, Juhyun Song

{"title":"Antitumor Effects of Metformin in Squamous Cell Carcinoma under Leptin Treatment Conditions.","authors":"Sujung Yeom, Danbi Jo, Seo Yoon Choi, Seo Yeon Ahn, Dong Hoon Lee, Juhyun Song","doi":"10.3746/pnf.2025.30.4.312","DOIUrl":"10.3746/pnf.2025.30.4.312","url":null,"abstract":"Sinonasal squamous cell carcinoma (SNSCC) is a rare, aggressive malignancy with poor clinical outcomes. Metabolic syndrome components, including obesity-associated hyperleptinemia, may promote tumor progression. Leptin is an adipokine that is elevated in obesity and activates oncogenic pathways that drive cancer cell proliferation. Although metformin exhibits anticancer effects in various malignancies, its specific role in SNSCC remains unclear. In this study, we examined the effects of leptin on SNSCC progression and the anticancer mechanisms of metformin in RPMI 2650 cells. We measured cell viability, proliferation, colony formation, and apoptosis following leptin and/or metformin exposure. Mitochondrial membrane potential assays and Ki-67 immunocytochemistry were used to assess mitochondrial function and proliferation, respectively. The results indicated that leptin promotes RPMI 2650 cell proliferation, colony formation, and survival by activating extracellular signal-regulated kinase (ERK) signaling. Conversely, metformin inhibited these leptin-induced oncogenic effects by suppressing ERK phosphorylation, reducing proliferation (confirmed by Ki-67 analysis), and inducing apoptosis. Metformin also modulated the tumor microenvironment by upregulating interleukin (IL)-2 and IL-18, while downregulating Serpin E1/plasminogen activator inhibitor-1, to potentially enhance the antitumor immune response. Furthermore, metformin induced mitochondrial dysfunction, reducing the membrane potential and inducing apoptosis. The results indicate that leptin is a potential driver of SNSCC progression and establish the antiproliferative and proapoptotic effects of metformin through the induction of mitochondrial dysfunction and ERK pathway inhibition. The ability of metformin to counteract leptin-driven tumor growth suggests its potential therapeutic use against SNSCC, particularly in patients with metabolic disorders.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"30 4","pages":"312-322"},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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