Preventive Nutrition and Food Science最新文献

Corrigendum: Determination of the Phytochemical Components, Nutritional Content, Biological Activities, and Cytotoxicity of Ripening Karanda (Carissa carandas) Fruit Extract for Functional Food Development. 勘误：功能性食品开发中成熟卡兰达（Carissa carandas）果实提取物的植物化学成分、营养成分、生物活性和细胞毒性的测定。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-30 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2026.01c

Supawadee Patathananone, Amonrat Koraneekij, Anuwat Wanthong, Wuttisak Kunu

引用次数: 0

Sugar-Sweetened Beverages Consumption among Koreans: Differences across Age Groups and Beverage Types. 韩国人的含糖饮料消费：不同年龄组和饮料类型的差异。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-23 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.255

Khongorzul Ganbat, Bo Hwi Kim, SuJin Song

{"title":"Sugar-Sweetened Beverages Consumption among Koreans: Differences across Age Groups and Beverage Types.","authors":"Khongorzul Ganbat, Bo Hwi Kim, SuJin Song","doi":"10.3746/pnf.2025.255","DOIUrl":"https://doi.org/10.3746/pnf.2025.255","url":null,"abstract":"The consumption of beverages has rapidly increased in Korea in recent years. However, such data on sugar-sweetened beverages (SSBs), which are one of the major dietary risks for cardiometabolic diseases, are very limited. This study evaluated SSBs consumption by sex and age group as well as SSBs types among Koreans. We included 17,084 participants (≥3 years) from the 2020－2022 Korea National Health and Nutrition Examination Surveys. Based on 24 h dietary recall data, SSBs were assessed as daily consumption (g/d and servings/d) and categorized as soft drinks, fruit drinks and juices, sweetened coffees, and other sweetened beverages. These analyses were also conducted separately with only SSBs consumers (n=8,404). The mean daily consumption of SSBs in the total study population was 96.5 g/d [standard error (SE)=2.4; mean intake=0.9 servings/d]. Among SSBs consumers, the mean consumption was 192.7 g/d (SE=4.2; mean intake=1.7 servings/d). Adolescents and young adults consumed more SSBs compared to other age groups. SSBs consumption differed by age group and beverage type, with fruit drinks and juices being most popular among children; soft drinks and other sweetened beverages among adolescents and young adults, and sweetened coffee among adults. Energy intake from SSBs was 74.2 and 75.9 kcal/d in adolescents and young adults, respectively; ∼20% of total daily sugar and ∼4% of total daily energy intake was from SSBs. This study found that the types and amounts of SSBs consumed vary by age group, with adolescents and young adults showing particularly higher consumption, highlighting the need for age-specific intervention strategies.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality and Functional Characteristics of Morning Bread Supplemented with Brewer's Spent Grain Powder. 添加啤酒糟粉的早面包品质及功能特性研究

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-23 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.259

Jisung Cheung, Eun-Sun Hwang

{"title":"Quality and Functional Characteristics of Morning Bread Supplemented with Brewer's Spent Grain Powder.","authors":"Jisung Cheung, Eun-Sun Hwang","doi":"10.3746/pnf.2025.259","DOIUrl":"https://doi.org/10.3746/pnf.2025.259","url":null,"abstract":"This study evaluated the physicochemical properties and antioxidant activity of morning bread prepared by substituting 10%－30% wheat flour with brewer's spent grain (BSG) powder. The effects of BSG on the baking characteristics were assessed. Bread weight increased from 34.83 to 36.03 g with elevating BSG levels, whereas height and volume decreased; specifically, the height of bread with 30% BSG reduced by 16.66%, restuling in the lowest specific volume. Baking loss declined from 12.92% to 9.92% as the BSG content increased. Texture profile analysis revealed that bread hardness increased with BSG addition, reaching 861.00 g at the 30% level Cohesiveness decreased, while chewiness peaked in the 30% BSG bread. Color examination indicated that crust and crumb lightness declined, whereas redness and yellowness increased, resulting in a dark-red appearance. Soluble solids increased from 4.30 to 4.95 °Brix, pH reduced from 5.85 to 5.75, and total titratable acidity rose from 0.11 to 0.12－0.15 with higher BSG levels. Moisture and crude protein contents increased from 30.35% to 33.39% and 8.60% to 12.18%, respectively. Ash and total dietary fiber were also elevated, while crude fat remained unchanged. Total polyphenol and flavonoid contents significantly improved from 49.06 to 142.02 µg gallic acid equivalent/g and 43.87 to 75.47 µg catechin equivalent/g, respectively. Antioxidant activity also markedly improved with BSG addition. Overall, substituting 10% of wheat flour with BSG is recommended to optimize baking quality, physicochemical properties, and functional value, demonstrating BSG's potential as a functional ingredient.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147818981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenolic Composition Profile and Antidiabetic Activities of Jack Bean [Canavalia ensiformis (L.) DC.] Tempeh. 大豆酚类成分及抗糖尿病活性研究直流。豆豉。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-23 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.286

Tamanda Sekar Setyani, Fiametta Ayu Purwandari, Nualpun Sirinupong, Worrapanit Chansuwan

{"title":"Phenolic Composition Profile and Antidiabetic Activities of Jack Bean [Canavalia ensiformis (L.) DC.] Tempeh.","authors":"Tamanda Sekar Setyani, Fiametta Ayu Purwandari, Nualpun Sirinupong, Worrapanit Chansuwan","doi":"10.3746/pnf.2025.286","DOIUrl":"https://doi.org/10.3746/pnf.2025.286","url":null,"abstract":"This study examined the effects of fermentation with Rhizopus oligosporus on cell wall structure and permeability, phenolic compound bioavailability, and antidiabetic potential of jack bean [Canavalia ensiformis (L.) DC.] tempeh. Fermentation significantly increased the total phenolic (∼21%) and flavonoid (∼152%) contents, mainly through the release of bound phenolics. Confocal fluorescence scanning microscopy with calcofluor white staining and fluorescein isothiocyanate-dextran probe permeation revealed the disruption of cellulose linkages in jack bean tempeh, which increased permeability and facilitated the diffusion of larger molecules. LC-ESI-QTOF-MS/MS identified 102 compounds across the free, conjugated, and bound phenolic fractions, with the conjugated fraction showing the most diverse composition. Certain bioactive compounds, such as D-(+)-3-phenyllactic acid, apigenin-7-O-glucoside, luteolin-7-neohesperidoside, kaempferol, arbutin, and oleacein, were tentatively linked to α-amylase and α-glucosidase inhibition. The free phenolic fraction exhibited the strongest α-amylase inhibition [half maximal inhibitory concentration (IC50)=35.75±0.91 µg/mL], whereas the conjugated and bound fractions were most effective against α-glucosidase (IC50=353.70±119.79 and 56.33±10.40 µg/mL, respectively). Correlation analysis revealed positive associations between phenolic content and α-amylase inhibition (r>0.8, P<0.05). In summary, fermentation improved cell wall permeability, the release of bound phenolics, and antidiabetic potential, suggesting that jack bean tempeh, rich in bioaccessible phenolics, is a potential functional food for glycemic regulation.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing Caenorhabditis elegans as an In Vivo Model for Identifying Small Molecules Modulating Lipid Metabolism: A Guideline. 利用秀丽隐杆线虫作为体内模型鉴定调节脂质代谢的小分子：指南。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-20 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.322

Zixuan Teng, Aaron Taehwan Kim, Peiyi Shen, Soonkyu Chung, Yeonhwa Park

{"title":"Utilizing Caenorhabditis elegans as an In Vivo Model for Identifying Small Molecules Modulating Lipid Metabolism: A Guideline.","authors":"Zixuan Teng, Aaron Taehwan Kim, Peiyi Shen, Soonkyu Chung, Yeonhwa Park","doi":"10.3746/pnf.2025.322","DOIUrl":"https://doi.org/10.3746/pnf.2025.322","url":null,"abstract":"Caenorhabditis elegans is a multi-organ nematode widely used in various research fields of life science. It is favored for its ease of handling, short life cycle with high reproductive capacity, low maintenance costs, availability of diverse mutants, and substantial genomic and proteomic similarity to mammals. Since research involving C. elegans typically does not require approval from the Institutional Animal Care and Use Committee, it serves as a valuable alternative to vertebrate animal models. Most importantly, lipid metabolism exhibits significant homology between C. elegans and mammals, making it a powerful in vivo model for studying many food-based bioactives that target lipid metabolism. This review briefly covers the basic physiology and genes involved in lipid metabolism in C. elegans, research procedures, and limitations of using its use in research. In addition to established models like mammalian tissue culture and rodents, utilizing C. elegans can be a valuable strategy for exploring the potential role of food-based bioactives in controlling obesity.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids of Cuscuta chinensis Enhance Osteogenic Differentiation in Dental Pulp Stem Cells via the Regulation of Wnt/β-Catenin Signaling. 菟丝子黄酮通过调控Wnt/β-Catenin信号通路促进牙髓干细胞成骨分化

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-20 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.243

Jianghua Yang, Xiyuan Guo, Jingang Xiao, Fahsai Kantawong

{"title":"Flavonoids of Cuscuta chinensis Enhance Osteogenic Differentiation in Dental Pulp Stem Cells via the Regulation of Wnt/β-Catenin Signaling.","authors":"Jianghua Yang, Xiyuan Guo, Jingang Xiao, Fahsai Kantawong","doi":"10.3746/pnf.2025.243","DOIUrl":"https://doi.org/10.3746/pnf.2025.243","url":null,"abstract":"Osteoporosis renders postmenopausal women susceptible to alveolar bone loss and complicates dental care, highlighting the urgent need for devising safe and effective nutritional strategies that support bone health. Cuscuta chinensis, a traditional medicinal plant, contains flavonoids with reported bioactivity on bone metabolism. This study aimed to evaluate the osteogenic potential of the total flavonoids of C. chinensis (TFCC) using human dental pulp stem cells (HDPSCs) and establish their preventive relevance for osteoporosis management. HDPSCs were treated with TFCC, and osteogenic differentiation was assessed employing alkaline phosphatase activity, Alizarin red S staining, wound-healing assay, RNA sequencing, and Western blotting of Wnt/β-catenin pathway proteins. TFCC at low concentrations enhanced HDPSC proliferation and markedly promoted osteogenic differentiation, as evidenced by elevated mineralized nodule formation and accelerated wound closure. Transcriptomic and protein analyses confirmed the activation of the Wnt/β-catenin signaling pathway, including an upregulation of runt-related transcription factor 2, osteopontin, β-catenin, and lymphoid enhancer-binding factor 1, with a concomitant phosphorylation of glycogen synthase kinase-3 β. Additionally, TFCC also mitigated the inhibitory effects of the Wnt inhibitor XAV-939. These findings indicate enhanced osteogenic differentiation in response to TFCC treatment, a process that may involve Wnt/β-catenin pathway modulation. Our results suggest that TFCC may serve as a diet component for maintaining bone health in menopausal women, offering new translational potential for osteoporosis-related dental care.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermented Oyster Extract Improves Linear Growth in Children with Idiopathic Short Stature: A Randomized, Double-Blind, Placebo-Controlled Trial. 发酵牡蛎提取物改善特发性身材矮小儿童的线性生长：一项随机、双盲、安慰剂对照试验。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-20 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.305

D P Nagahawatta, Fengqi Yang, N M Liyanage, Kyoung-Min Rheu, Bae-Jin Lee, Ki Bong Kim, You-Jin Jeon

{"title":"Fermented Oyster Extract Improves Linear Growth in Children with Idiopathic Short Stature: A Randomized, Double-Blind, Placebo-Controlled Trial.","authors":"D P Nagahawatta, Fengqi Yang, N M Liyanage, Kyoung-Min Rheu, Bae-Jin Lee, Ki Bong Kim, You-Jin Jeon","doi":"10.3746/pnf.2025.305","DOIUrl":"https://doi.org/10.3746/pnf.2025.305","url":null,"abstract":"This randomized, double-blind, placebo-controlled clinical trial evaluated the efficacy and safety of fermented oyster extract (FGO) as a functional food ingredient for promoting growth in children with idiopathic short stature (ISS). In total, 80 children aged 6－9 years with height below the 25th percentile for age and gender per the Korean National Growth Charts, 2017, were included. They were randomly assigned to receive either 500 mg/d FGO or a matched placebo for 24 weeks. The primary outcome was a change in height while standing; secondary outcomes included growth rate, height standard deviation score (height-SDS), bone age (BA), and levels of growth-related hormones and bone metabolism markers. In the intention-to-treat analysis, mean height gain at 24 weeks was 3.22±0.71 and 2.38±0.74 cm in the FGO and placebo groups (P<0.001). Growth rate, height-SDS, BA, serum insulin-like growth factor-1, and urinary deoxypyridinoline increased significantly in the FGO group compared with the placebo group (P<0.05). In contrast, growth hormone and insulin-like growth factor-binding protein-3 levels did not vary between groups. No serious adverse events or clinically meaningful laboratory abnormalities were observed; however, two events of mild rash, possibly related to FGO, led to one drop-out. These findings suggest that standardized FGO may safely augment linear growth and bone metabolism in children with ISS. This product could be developed as a marine-derived functional food for pediatric growth support, although more long-term and multicenter studies are warranted to confirm the generalizability of these effects and FGO durability.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA-Protective Effects of Crocus sativus Stamen Extracts and Their Safety Assessment for Use as Natural Bioactive Agents. 藏红花雄蕊提取物对dna的保护作用及其作为天然生物活性剂的安全性评价

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-13 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.258

Samira Mamri, Sanae Baddaoui, Oussama Khibech, Sabir Ouahhoud, Amine Khoulati, Mohammed Choukri, Abdeslam Asehraou, Ennouamane Saalaoui

{"title":"DNA-Protective Effects of Crocus sativus Stamen Extracts and Their Safety Assessment for Use as Natural Bioactive Agents.","authors":"Samira Mamri, Sanae Baddaoui, Oussama Khibech, Sabir Ouahhoud, Amine Khoulati, Mohammed Choukri, Abdeslam Asehraou, Ennouamane Saalaoui","doi":"10.3746/pnf.2025.258","DOIUrl":"https://doi.org/10.3746/pnf.2025.258","url":null,"abstract":"The stamens of Crocus sativus L., often discarded as floral waste, are rich in flavonoids and phenolics. This study investigated their toxicological safety, antigenotoxic potential, and molecular mechanisms of protection against cyclophosphamide (CP)-induced genotoxicity. Hydroethanolic and hydromethanolic extracts were prepared, and acute oral toxicity was assessed in mice following the Organization for Economic Co-operation and Development guideline 423. Genotoxicity and its amelioration were evaluated in rat and mouse leukocytes by employing the alkaline comet assay. Oxidative stress markers, including superoxide dismutase activity, as well as malondialdehyde and glutathione levels, in hepatic and renal tissues were quantified. In parallel, nine major metabolites were identified in the extracts and were molecularly docked with key enzymes involved in CP bioactivation (CYP2B6), aldehyde detoxification (ALDH1A1), DNA repair (OGG1), and oxidative stress regulation (Keap1-Kelch domain; Protein Data Bank ID: 7K2A). The extracts were non-toxic up to 4,000 mg/kg and did not exhibit any genotoxicity. Pre-treatment with extracts (200 mg/kg body weight) significantly attenuated CP-induced DNA damage and restored antioxidant enzyme levels. Docking results supported these observations: rutin demonstrated a high affinity for CYP2B6 (-11.2 kcal/mol) and favorable binding to 7K2A (-9.7 kcal/mol); catechin gallate bound tightly to ALDH1A1 (-10.0 kcal/mol) and OGG1 (-9.2 kcal/mol). These findings suggest reduced CP activation, but enhanced detoxification and DNA repair. In conclusion, the extracts of C. sativus stamens are safe and possess robust antioxidant and antigenotoxic properties, confirmed by molecular docking. These findings highlight their potential as natural protective agents against chemotherapy-induced genotoxicity.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of the Antioxidant and Anti-Inflammatory Effects of Jeju Beetroot (Beta vulgaris) through Repeated Steaming Cycles. 反复蒸煮对济州甜菜根抗氧化和抗炎作用的增强。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-13 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.274

Ye Yeong Hong, Ji Hun Byeon, Song-I Han, Jae-Hoon Kim

{"title":"Enhancement of the Antioxidant and Anti-Inflammatory Effects of Jeju Beetroot (Beta vulgaris) through Repeated Steaming Cycles.","authors":"Ye Yeong Hong, Ji Hun Byeon, Song-I Han, Jae-Hoon Kim","doi":"10.3746/pnf.2025.274","DOIUrl":"https://doi.org/10.3746/pnf.2025.274","url":null,"abstract":"Beetroot is known for its antioxidant and anti-inflammatory properties; however, its use as a processed food resource remains limited. This study investigated the effects of repeated steaming cycles on the antioxidant and anti-inflammatory properties of beetroot extracts. Repeated steaming of up to five cycles substantially enhanced its antioxidant activity, as evidenced by decreased IC50 values of the 2-diphenyl-1-picryhydrazyl radical scavenging assay and increased total phenolic content. In lipopolysaccharide-stimulated RAW 264.7 macrophages, steamed beetroot extracts substantially suppressed the production of nitric oxide and reduced the pro-inflammatory cytokine levels without inducing cytotoxicity. Furthermore, these anti-inflammatory effects were associated with the downregulation of inflammatory mediator-related signaling pathways. Overall, repeated steaming effectively enhanced the functional properties of beetroot, thereby supporting its potential use as an antioxidant and anti-inflammatory agent.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puerarin-Rich Pueraria lobata Extract Attenuates H₂O₂- and Dexamethasone-Induced Atrophy in C2C12 Myoblasts and Myotubes. 富葛根素的葛根提取物减轻H2O2和地塞米松诱导的C2C12肌母细胞和肌管萎缩。

IF 2.5

Preventive Nutrition and Food Science Pub Date : 2026-04-13 eCollection Date: 2026-04-01 DOI: 10.3746/pnf.2025.307

Young Han Kim, Jae In Jung, Soung Yong Park, Jae-Hoon Shim, Young Seung Lee, Seong Il Heo

{"title":"Puerarin-Rich Pueraria lobata Extract Attenuates H2O2- and Dexamethasone-Induced Atrophy in C2C12 Myoblasts and Myotubes.","authors":"Young Han Kim, Jae In Jung, Soung Yong Park, Jae-Hoon Shim, Young Seung Lee, Seong Il Heo","doi":"10.3746/pnf.2025.307","DOIUrl":"https://doi.org/10.3746/pnf.2025.307","url":null,"abstract":"Sarcopenia is an age-related decline in skeletal muscle mass and function that is closely associated with oxidative stress and excessive glucocorticoid exposure. This study investigated the protective effects of a Pueraria lobata extract powder (PEP) in C2C12 myotubes. PEP was standardized to 4.17% puerarin. PEP pretreatment significantly improved cell viability and suppressed the intracellular accumulation of reactive oxygen species under hydrogen peroxide-induced oxidative stress, while restoring the levels of glutathione and reducing apoptosis. Furthermore, in dexamethasone-treated myotubes, PEP pretreatment prevented myotube shrinkage, downregulated the E3 ubiquitin ligases Atrogin-1 and MuRF1, and upregulated the myogenic markers MyoD and myogenin. Mechanistically, PEP restored the phosphorylation of Akt and mTOR while enhancing the inhibition of FoxO3a phosphorylation, thereby promoting anabolic and suppressing catabolic signaling. These findings suggest that PEP serves dual protective functions through redox modulation and muscle protein metabolism regulation. This study provides in vitro evidence that a puerarin-rich P. lobata extract can concurrently mitigate oxidative stress and glucocorticoid-induced muscle atrophy, thus supporting its potential as a multi-targeted nutraceutical candidate for preventing muscle wasting and sarcopenia.","PeriodicalId":20424,"journal":{"name":"Preventive Nutrition and Food Science","volume":"31 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13125050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0