Phytopathology最新文献_第4页

How to Conduct Phylogenetic Endoglucanase (egl) Inference Using the Reference Ralstonia solanacearum Species Complex Curated Database?

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-11 DOI: 10.1094/PHYTO-10-24-0318-SC

Gilles Cellier, Miharisoa Mirana Gauche, Jean Jacques Cheron, Yann Pecrix

{"title":"How to Conduct Phylogenetic Endoglucanase (egl) Inference Using the Reference Ralstonia solanacearum Species Complex Curated Database?","authors":"Gilles Cellier, Miharisoa Mirana Gauche, Jean Jacques Cheron, Yann Pecrix","doi":"10.1094/PHYTO-10-24-0318-SC","DOIUrl":"https://doi.org/10.1094/PHYTO-10-24-0318-SC","url":null,"abstract":"The phytopathogenic Ralstonia solanacearum species complex (RSSC) was recently divided into three distinct species, after long-standing researches. About twenty years ago, phylotype-based classification was introduced to mark the beginning of DNA-based taxonomy within RSSC. Within each phylotype, the \"sequevar\" classification further refines subspecies designations, based on variations in the endoglucanase (egl) gene sequence, and these sequevars are unique to each phylotype. While a single-gene approach like egl is less comprehensive than multi-gene or whole-genome analysis, egl phylogenetic inferences provides a robust and cost-effective RSSC strain typing assessment. Curated and public egl reference sequences are essential for accurate sequevar assignment of unknown RSSC strains and help prevent issues related to incorrect sequevar assignment or trimming errors that could compromise the quality of RSSC diversity research. Our research proposed to fill the gap by providing such database to the RSSC community (https://doi.org/10.18167/DVN1/CUWA5P or https://tinyurl.com/sequevar), along with a proper methodology to perform reproducible and reliable phylogenetic inferences for publishing sequevar assignation.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Analysis Reveals That a Gti1/Pac2 Family Gene, CpSge1, Regulates Fungal Growth, Stress Response, and Virulence in Cryphonectria parasitica.

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-10 DOI: 10.1094/PHYTO-11-24-0354-R

Xiaorong Lin, Yuchen Yang, Can Huang, Dianguang Xiong, Xiaolin Qiu, Chengming Tian

{"title":"Transcriptome Analysis Reveals That a Gti1/Pac2 Family Gene, CpSge1, Regulates Fungal Growth, Stress Response, and Virulence in Cryphonectria parasitica.","authors":"Xiaorong Lin, Yuchen Yang, Can Huang, Dianguang Xiong, Xiaolin Qiu, Chengming Tian","doi":"10.1094/PHYTO-11-24-0354-R","DOIUrl":"https://doi.org/10.1094/PHYTO-11-24-0354-R","url":null,"abstract":"The Gti1/Pac2 family comprises crucial transcription factors widely distributed in fungi with generally two members Gti1 (also known as Wor1, Ryp1 or Sge1) and Pac2, where the Gti1 homologues play significant roles in growth, spore production, and pathogenicity of various pathogenic fungi. Despite its recognized significance, the roles of this family in Cryphonectria parasitica, the pathogen responsible for chestnut blight (a globally significant forest disease) remain unexplored. In this study, CpSge1 was identified in C. parasitica and then knocked out to explore its functions. The results showed that CpSge1 significantly affected vegetative growth, conidiation, hydrophobicity, and stress tolerance of C. parasitica. Notably, the CpSge1 deletion mutants were significantly less pathogenic compared to the wild type. Transcriptomic analysis of the wild type and the CpSge1 deletion mutant during vegetative growth and infection stages revealed that CpSge1 regulated a number of pathogenicity-related genes in C. parasitica. Yeast one hybrid verified the direct binding of CpSge1 to the promoter regions of genes encoding pectin lyase CpPL1 and major facilitator superfamily transporter CpMF1. In summary, these data suggest that CpSge1 is the core regulator of fungal growth, stress tolerance, gene expression, and virulence in C. parasitica, which may improve our understanding on the molecular pathogenesis of C. parasitica and then help us to develop effective control strategies.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Insect Vector, Diaphorina citri Kuwayama Exhibits a Lower Level of Fatty Acids Upon Infection with the Phytopathogenic Bacterium 'Candidatus Liberibacter asiaticus'.

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-10 DOI: 10.1094/PHYTO-10-24-0336-R

Nabil Killiny, Mahnaz Rashidi

{"title":"The Insect Vector, Diaphorina citri Kuwayama Exhibits a Lower Level of Fatty Acids Upon Infection with the Phytopathogenic Bacterium 'Candidatus Liberibacter asiaticus'.","authors":"Nabil Killiny, Mahnaz Rashidi","doi":"10.1094/PHYTO-10-24-0336-R","DOIUrl":"https://doi.org/10.1094/PHYTO-10-24-0336-R","url":null,"abstract":"Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the main vector for the bacterium 'Candidatus Liberibacter asiaticus', which is associated with citrus greening, also known as Huanglongbing. D. citri transmits 'Ca. L. asiaticus' during its feeding on citrus phloem sap. Transmission occurs in a circulative, propagative, and persistent manner. 'Ca. L. asiaticus' has a small genome (1.2 Mb). Therefore, it acquires most of its nutrients and energetic nucleotides from its hosts. The objective of this study was to assess the effect of 'Ca. L. asiaticus' infection on the level of the free fatty acids in its vector. The fatty acids were extracted from adult D. citri using ethyl acetate, derivatized with boron trifluoride-methanol, and analyzed using gas chromatography-mass spectrometry (GC-MS). Nine fatty acids were identified in the D. citri extracts. Oleic acid was the most predominant fatty acid followed by stearic and palmitic acid, whereas the rest of the fatty acids were present in low amounts. In general, the levels of the detected fatty acids in 'Ca. L. asiaticus'-infected D. citri were lower than those found in healthy psyllids. Our findings showed that, the reduction of fatty acids in 'Ca. L. asiaticus'-infected psyllids resulted from the higher activity of β-oxidation to generate acetyl-CoA, which causes more production of ATP. Our results indicated that 'Ca. L. asiaticus' may enhance the β-oxidation of fatty acids in its vector insect to fulfill its nutrient and energetic nucleotide requirements.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosyltransferase-Like Toxin of Burkholderia gladioli Strain NGJ1 Is a Potent Antifungal Protein with Potential for Control of Sheath Blight Disease in Rice.

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-06 DOI: 10.1094/PHYTO-12-24-0383-R

Amrita Pradhan, Sunil K Yadav, Gopaljee Jha

{"title":"Glycosyltransferase-Like Toxin of Burkholderia gladioli Strain NGJ1 Is a Potent Antifungal Protein with Potential for Control of Sheath Blight Disease in Rice.","authors":"Amrita Pradhan, Sunil K Yadav, Gopaljee Jha","doi":"10.1094/PHYTO-12-24-0383-R","DOIUrl":"https://doi.org/10.1094/PHYTO-12-24-0383-R","url":null,"abstract":"Sheath blight disease caused by the fungal pathogen Rhizoctonia solani poses a great challenge for sustainable rice cultivation. It is important to develop environment-friendly measures for its control. Previously, a rice-associated Burkholderia gladioli strain NGJ1 has been shown to exhibit mycophagous and antifungal activity on R. solani. Here we report that a glucosyltransferase-like (BGT1) protein with a canonical DxD motif that is homologous to glycosyltransferase (GT) toxin of different bacteria is encoded in the antibacterial type VI secretion system (T6SS) encoding gene cluster of NGJ1. The recombinant BGT1 protein purified from Escherichia coli exhibits antifungal activity on R. solani, Magnaporthe oryzae, Fusarium oxysporum, Saccharomyces cerevisiae, and Candida albicans, under laboratory conditions. Using a variant of BGT1 protein (BGT1D168L/D170L), we demonstrate that DxD motif is important for its antifungal activity. The heterologous expression of native BGT1 but not BGT1D168L/D170L protein prevents the growth of yeast cells. Moreover, treatment with BGT1 but not BGT1D168L/D170L significantly reduces sheath blight disease severity in rice. BGT1 treatment does not elicit adverse effects on plants. In conclusion, we emphasize that BGT1 protein-based or transgene-based biotechnological interventions can be exploited for effective control of sheath blight disease in rice.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allicin Binds to Cysteine Residues of Fungal Dihydrolipoamide Dehydrogenase to Control Gray Mold (Botrytis cinerea) of Tomato.

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-04 DOI: 10.1094/PHYTO-10-24-0315-R

Dan Cheng, Fenghua Liu, Dongxue Li, Yue Ma, Jing-Jiang Zhou, Delu Wang, Zhuo Chen

{"title":"Allicin Binds to Cysteine Residues of Fungal Dihydrolipoamide Dehydrogenase to Control Gray Mold (Botrytis cinerea) of Tomato.","authors":"Dan Cheng, Fenghua Liu, Dongxue Li, Yue Ma, Jing-Jiang Zhou, Delu Wang, Zhuo Chen","doi":"10.1094/PHYTO-10-24-0315-R","DOIUrl":"https://doi.org/10.1094/PHYTO-10-24-0315-R","url":null,"abstract":"Allicin exhibits strong inhibitory activity against phytopathogenic fungi; however, its antifungal mechanism remains unclear. This study assessed allicin's inhibitory effects on several phytopathogenic fungi, revealing a half-maximal effective concentration of 125.47 μg/ml against the hyphal growth of Botrytis cinerea. Micromorphological analysis showed that allicin caused abnormalities in the hyphae, including unclear organelle boundaries and organelle dissolution. Integrated transcriptomic, and proteomic, and metabolomic assays indicated that allicin induced differential gene and protein expression, particularly in the plasma membrane, oxidative stress processes, and energy metabolism pathways. Additionally, differentially expressed metabolites were involved in the inhibition of hyphal growth. Biochemical assays demonstrated that allicin inhibited ATP production and damaged hyphal cell membranes. Molecular docking revealed that allicin could bind to dihydrolipoamide dehydrogenase (DLDH), an enzyme rich in cysteine residues, with a binding free energy of -6.322 kcal/mol. Through antimicrobial activity testing of allicin analogues and molecular docking analysis, the active groups of allicin and its interaction with DLDH were identified. This study shows that allicin interferes with energy metabolism, impacts cell membrane and wall integrity by targeting cysteine-containing proteins, and inhibits the proliferation of plant pathogenic fungi. These insights into the antifungal mechanism will provide valuable data for the development and field application of allicin analogues.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overwintering Capability of Xanthomonas arboricola pv. pruni Strains with Different Bactericidal Sensitivities on Peach Trees.

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-04 DOI: 10.1094/PHYTO-09-24-0298-R

Milan Panth, Enoch Noh, Alec Sherratt, Guido Schnabel, Daniel J Anco, Renato Carvalho, Jeffrey B Jones, Hehe Wang

{"title":"Overwintering Capability of Xanthomonas arboricola pv. pruni Strains with Different Bactericidal Sensitivities on Peach Trees.","authors":"Milan Panth, Enoch Noh, Alec Sherratt, Guido Schnabel, Daniel J Anco, Renato Carvalho, Jeffrey B Jones, Hehe Wang","doi":"10.1094/PHYTO-09-24-0298-R","DOIUrl":"https://doi.org/10.1094/PHYTO-09-24-0298-R","url":null,"abstract":"Xanthomonas arboricola pv. pruni (XAP) causes bacterial spot of peach and is primarily managed in commercial South Carolina peach orchards with copper and oxytetracycline applications. The pathogen overwinters in peach twigs and forms spring cankers which generate primary inoculum for early-season leaf and fruitlet infections. Copper-tolerant and oxytetracycline-resistant strains have recently been reported, but their overwintering capability in the absence of selection pressure is unknown. Similarly, the impact of infection during different phenological stages on XAP overwintering is unknown. To investigate these factors, peach twigs were sprayed with strains of XAP with different sensitivities to copper and oxytetracycline in an experimental orchard during bud set (June), after harvest (August), and during leaf drop (October) in the fall. Viable XAP from peach buds was quantified using qPCR with or without propidium monoazide (PMA) treatment. Results indicated that bud cankers were a major inoculum reservoir for XAP, and the overwintered XAP densities in buds were significantly and positively correlated with spring canker ratings (R2 = ~0.6, p < 0.001). Inoculation of peach twigs at all three time points yielded overwintering XAP populations and canker symptoms in spring, with the highest amount of canker and XAP from the leaf drop [42.7% canker incidence, 31,258 CFU/bud] and after harvest [30.4% canker incidence, 8,511 CFU/bud] inoculations. There were no significant differences in overwintering capability of XAP strains in the absence of copper or oxytetracycline sprays, suggesting that these oxytetracycline-resistant and copper-tolerant strains may persist and accumulate in peach orchards across seasons.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotype-Specific Responses of Common Bean to Meloidogyne incognita. 普通豆对黑僵菌的基因型特异性反应

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-04 DOI: 10.1094/PHYTO-10-24-0313-R

Nicole Orsi, João Paulo Rodrigues Marques, Líllian Beatriz Januário Bibiano, Luis Eduardo Aranha Camargo, Daniel Guariz Pinheiro, Maria Lucia Carneiro Vieira

{"title":"Genotype-Specific Responses of Common Bean to Meloidogyne incognita.","authors":"Nicole Orsi, João Paulo Rodrigues Marques, Líllian Beatriz Januário Bibiano, Luis Eduardo Aranha Camargo, Daniel Guariz Pinheiro, Maria Lucia Carneiro Vieira","doi":"10.1094/PHYTO-10-24-0313-R","DOIUrl":"https://doi.org/10.1094/PHYTO-10-24-0313-R","url":null,"abstract":"The root-knot nematode Meloidogyne incognita causes large galls on roots, interfering with the flow of water and nutrients to the plant. In the common bean, no major resistance (R) genes have been described. Instead, resistance is controlled by multiple genes, which have not proved effective so far. An RNA-seq approach was used to identify genes involved in common bean response to M. incognita at the stages of nematode invasion and root-galling. When comparing infected and uninfected treatments of a moderately resistant (MR) and a susceptible (S) genotype, several genes were identified as differentially expressed. Their functional annotation indicated that both genotypes underwent complex transcriptional reprogramming from early to later periods of the interaction, but defense-related genes were mostly upregulated in the MR genotype. At the early stage, a large set of genes was activated in both genotypes, including those involved in cell wall organization, signaling, hormonal pathways, transcription factors, oxidative stress and putative resistance gene analogs. Later, most of the previously activated defense mechanisms were no longer expressed in the S genotype. There was an increased expression of genes encoding proteins involved in hormonal signaling pathways (salicylic acid and gibberellin-related), protein kinases, transcription factors and oxidative stress in MR. However, a decreased expression of genes involved in signaling mediated by calcium and oxidative stress occurred in S, indicating susceptibility. The repertoire of genes identified herein will facilitate research in plant-nematode interactions, with possible applications for the improvement of the common bean.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the Power of Electrical Penetration Graph (EPG) Technology to Understand Psyllid-Transmitted Fastidious Bacterial Diseases.

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-01 DOI: 10.1094/PHYTO-11-24-0376-RVW

Nabil Killiny, Alberto Fereres

{"title":"Harnessing the Power of Electrical Penetration Graph (EPG) Technology to Understand Psyllid-Transmitted Fastidious Bacterial Diseases.","authors":"Nabil Killiny, Alberto Fereres","doi":"10.1094/PHYTO-11-24-0376-RVW","DOIUrl":"https://doi.org/10.1094/PHYTO-11-24-0376-RVW","url":null,"abstract":"Psyllids, also called plant lice, are hemipteran insects that feed on phloem sap. In addition to the direct damage they cause to plants, they are vectors of many phloem-restricted bacterial pathogens belonging to the 'Candidatus Liberibacter' spp. and 'Candidatus Phytoplasma' spp. from the apple proliferation group (16SrX). Although 'Candidatus Liberibacter' spp. cells possess cell walls unlike the phytoplasmas, they both share a reduced genome and unavailability in culture. In addition, psyllids transmit both species of bacteria in a persistent, circulative, and propagative manner. Because of the similarity of these pathosystems, electrical penetration graph (EPG) was employed to study the probing behavior of psyllids. Such studies may assist in understanding the specific interactions between the fastidious bacteria, plant hosts, and insect vectors and lead to innovative control strategies. Herein, we discuss the potential of EPG to study and understand the tritrophic interactions that secure a successful transmission from plant to plant. In addition, the use of EPG in evaluating psyllid control strategies including pesticides and tolerant varieties is reviewed.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyriofenone Interacts with the Major Facilitator Superfamily Transporter of Phytopathogenic Fungi to Potentially Control Tea Leaf Spot Caused by Lasiodiplodia theobromae. Pyriofenone 与植物病原真菌的主要促进剂超家族转运体相互作用，有可能控制由 Lasiodiplodia theobromae 引起的茶叶斑点病。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-01 Epub Date: 2025-01-27 DOI: 10.1094/PHYTO-08-24-0246-R

Xiaolin Zhang, Fenghua Liu, Dongxue Li, Di Guo, Yue Ma, Jing-Jiang Zhou, Delu Wang, Zhuo Chen

{"title":"Pyriofenone Interacts with the Major Facilitator Superfamily Transporter of Phytopathogenic Fungi to Potentially Control Tea Leaf Spot Caused by Lasiodiplodia theobromae.","authors":"Xiaolin Zhang, Fenghua Liu, Dongxue Li, Di Guo, Yue Ma, Jing-Jiang Zhou, Delu Wang, Zhuo Chen","doi":"10.1094/PHYTO-08-24-0246-R","DOIUrl":"10.1094/PHYTO-08-24-0246-R","url":null,"abstract":"Tea leaf spot caused by Lasiodiplodia theobromae is a newly discovered fungal disease in southwest China. Due to a lack of knowledge of its epidemiology and control strategies, the disease has a marked impact on tea yield and quality. Pyriofenone is a new fungicide belonging to the aryl phenyl ketone fungicide group, which has shown marked efficacy in controlling various fungal diseases. However, its mechanism of action is not yet understood. This study found that pyriofenone exhibits strong in vitro inhibitory activity against various phytopathogenic fungi. Specifically, it showed strong inhibitory activity against L. theobromae, with a half-maximal effective concentration (EC50) value of 0.428 μg/ml determined by measuring the mycelial growth rate. Morphological observations, using optical, scanning electron, and transmission electron microscopy, revealed that pyriofenone induces morphological abnormalities in L. theobromae hyphae. At lower doses, the hyphae became swollen, the distance between septa decreased, and the hyphal growth rate slowed. At higher doses and longer exposures, the hyphae collapsed. Transcriptomic and bioinformatic analyses indicated that pyriofenone can affect the expression of genes related to membrane transporters. Homology modeling suggested that pyriofenone may bind to a candidate target protein of the major facilitator superfamily transporter, with a free binding energy of -7.1 kcal/mol. This study suggests that pyriofenone may potentially regulate the transport of metabolites in L. theobromae, thus affecting hyphal metabolism and interfering with hyphal growth. Pyriofenone exhibits in vitro inhibitory activity against various tea foliar pathogens and holds promise for future applications to the control of tea foliar diseases.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"128-138"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenazine-1-Carboxamide Regulates Pyruvate Dehydrogenase of Phytopathogenic Fungi to Control Tea Leaf Spot Caused by Didymella segeticola. 吩嗪-1-甲酰胺调节植物病原真菌的丙酮酸脱氢酶，以控制由Didymella segeticola引起的茶叶斑点病。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2025-02-01 Epub Date: 2025-02-24 DOI: 10.1094/PHYTO-07-24-0209-R

Zeqi Qi, Fenghua Liu, Dongxue Li, Jiayu Yin, Delu Wang, Nazeer Ahmed, Yue Ma, Jing-Jiang Zhou, Zhuo Chen

{"title":"Phenazine-1-Carboxamide Regulates Pyruvate Dehydrogenase of Phytopathogenic Fungi to Control Tea Leaf Spot Caused by Didymella segeticola.","authors":"Zeqi Qi, Fenghua Liu, Dongxue Li, Jiayu Yin, Delu Wang, Nazeer Ahmed, Yue Ma, Jing-Jiang Zhou, Zhuo Chen","doi":"10.1094/PHYTO-07-24-0209-R","DOIUrl":"10.1094/PHYTO-07-24-0209-R","url":null,"abstract":"Due to a lack of understanding of the disease epidemiology and comprehensive control measures, tea leaf spot caused by Didymella segeticola has a significant negative impact on tea yield and quality in the tea plantations of Southwest China. Phenazine-1-carboxamide (PCN) is a phenazine compound derived from Pseudomonas species that exhibits antimicrobial activity against various pathogens. However, its inhibitory mechanism is not yet clear. The current study evaluated the inhibitory activity of PCN against various phytopathogenic fungi and found that PCN has inhibitory activity against multiple pathogens, with a half-maximal effective concentration value for D. segeticola of 16.11 μg/ml in vitro and a maximum in-vivo curative activity of 72.28% toward tea leaf spot. Morphological changes in the hyphae after exposure to PCN were observed through microstructure and ultrastructure analysis and indicated that PCN causes abnormalities in the hyphae, such as cytoplasmic coagulation, shortened hyphal inter-septum distances, and unclear boundaries of organelles. Transcriptomic analysis revealed that PCN upregulated the expression of genes related to energy metabolism. PCN significantly reduced the ATP concentration in the hyphae and decreased mitochondrial membrane potential. Molecular docking analysis indicated that PCN binds to one of the candidate target proteins, pyruvate dehydrogenase, with lower free energy of -10.7 kcal/mol. This study indicated that PCN can interfere with energy metabolism, reducing ATP generation and ultimately affecting hyphal growth. Overall, PCN shows potential for future application in the control of tea leaf spot due to its excellent antifungal activity and unique mode of action.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"139-150"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0