Phytopathology最新文献_第4页

NIb Interaction Sites ³⁵⁹⁷AUU and ³⁶⁰⁷GGCU Are Crucial to the Replication of Wheat Yellow Mosaic Virus RNA2. NIb相互作用位点3597AUU和3607GGCU对小麦黄花叶病毒RNA2的复制至关重要。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-07 DOI: 10.1094/PHYTO-05-25-0158-SC

Guowei Geng, Minjun Liu, Xueyuan Wang, Xuefeng Yuan

引用次数: 0

Population Genomic Analysis of Two Independent Clonal Invasions of the Sudden Oak Death Pathogen into One Forest. 橡树猝死病菌两次独立无性系入侵同一森林的种群基因组分析。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-02 DOI: 10.1094/PHYTO-10-24-0329-FI

Nicholas C Cauldron, Hazel A Daniels, Jared M LeBoldus, Niklaus J Grünwald

{"title":"Population Genomic Analysis of Two Independent Clonal Invasions of the Sudden Oak Death Pathogen into One Forest.","authors":"Nicholas C Cauldron, Hazel A Daniels, Jared M LeBoldus, Niklaus J Grünwald","doi":"10.1094/PHYTO-10-24-0329-FI","DOIUrl":"10.1094/PHYTO-10-24-0329-FI","url":null,"abstract":"Upon introduction, clonal pathogen populations are expected to go through a genetic bottleneck followed by gradual clonal divergence. Two distinct and purely clonal lineages of the sudden oak death pathogen Phytophthora ramorum recently emerged in forests in the Western United States, providing the unique opportunity to study a naturally replicated invasion into the same ecosystem. We characterized population genomic patterns during early invasion using whole-genome sequencing of two P. ramorum clonal lineages sampled in the first 5 years following their detection. We re-sequenced genomes from populations of two dominant clonal lineages, NA1 (n = 134; 2001 to 2005) and EU1 (n = 160; 2015 to 2019), and obtained 106,070 high-quality single-nucleotide polymorphisms in genic regions. Our results are consistent with the hypothesis of one introduction for each lineage. The NA1 population had a wider distribution of pairwise genetic distances than EU1 and higher genetic diversity, though neither NA1 nor EU1 populations clustered clearly by year. There was significant correlation between genetic distance and geographic distance for NA1 (P = 0.042), but not for EU1 (P = 0.402). The genetic diversity in NA1 is strongly driven by loss of heterozygous positions, which impacted more than one-third of the sampled NA1 population. However, loss of heterozygosity was rare in EU1. This work provides novel insights into the invasion biology and dynamics of clonal plant pathogens in natural ecosystems.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"PHYTO10240329FI"},"PeriodicalIF":3.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Unconventionally Secreted Effector, VmUSP1, Targets Apple Heat Shock Protein 70 to Promote Infection. 一种非常规分泌的效应物VmUSP1靶向苹果热休克蛋白70促进感染。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 Epub Date: 2025-09-09 DOI: 10.1094/PHYTO-12-24-0417-R

Chengli Wang, Tao Jiang, Meilian Li, Yonghui Lin, Ming Xu, Xuejing Wen, Daoyuan Zhang, Lili Huang

{"title":"An Unconventionally Secreted Effector, VmUSP1, Targets Apple Heat Shock Protein 70 to Promote Infection.","authors":"Chengli Wang, Tao Jiang, Meilian Li, Yonghui Lin, Ming Xu, Xuejing Wen, Daoyuan Zhang, Lili Huang","doi":"10.1094/PHYTO-12-24-0417-R","DOIUrl":"10.1094/PHYTO-12-24-0417-R","url":null,"abstract":"Apple Valsa canker, caused by Valsa mali/Cytospora mali, is a highly destructive disease that leads to significant economic losses annually. Pathogens manipulate host immunity to facilitate colonization and infection through the secretion of effector proteins, which are typically identified based on the presence of signal peptides. However, unconventional secretory effector proteins have been neglected, and little is known about their protogenetic roles in virulence. In this study, we demonstrate that an unconventional secreted protein 1 (VmUSP1) not only inhibits BAX- and INF1-induced cell death but also plays a crucial role in the complete virulence of V. mali. Furthermore, VmUSP1 lacks a typical signal peptide and exhibits characteristics of unconventional secretion. Through yeast two-hybrid, bimolecular fluorescence, and co-immunoprecipitation assays, we confirmed that VmUSP1 targets an apple (Malus × domestica) heat shock protein 70 (MdHSP70). MdHSP70 induces the accumulation of reactive oxygen species and callose while significantly enhancing plant resistance against pathogens. Additionally, VmUSP1 greatly compromises the MdHSP70-mediated resistance of apple against V. mali. Overall, these findings elucidate a mechanism by which an unconventionally secreted effector from V. mali suppresses host resistance by interfering with MdHSP70-mediated immune responses.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1194-1203"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Draft Genome of 'Candidatus Phytoplasma pyri' and Phylogenetic Diversity Among Chilean and Italian Strains. “pyri候选植物原体”基因组草图及智利和意大利菌株的系统发育多样性。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 Epub Date: 2025-09-08 DOI: 10.1094/PHYTO-01-25-0041-SC

Weier Cui, Carlos Alburquenque, Francesco Pacini, Constanza González, Pietro Bianco, Sebastian Cabrera, Tomás Llantén, Javiera Fuentes, Camila Gamboa, Assunta Bertaccini, Nicola Fiore, Alan Zamorano

{"title":"Draft Genome of 'Candidatus Phytoplasma pyri' and Phylogenetic Diversity Among Chilean and Italian Strains.","authors":"Weier Cui, Carlos Alburquenque, Francesco Pacini, Constanza González, Pietro Bianco, Sebastian Cabrera, Tomás Llantén, Javiera Fuentes, Camila Gamboa, Assunta Bertaccini, Nicola Fiore, Alan Zamorano","doi":"10.1094/PHYTO-01-25-0041-SC","DOIUrl":"10.1094/PHYTO-01-25-0041-SC","url":null,"abstract":"'Candidatus Phytoplasma pyri', the pathogen associated with pear decline, affects pear trees across both the old and new worlds. However, research on this phytoplasma has been limited by the lack of genomic data. This study presents the first draft genome of 'Ca. P. pyri' using a strain from Chile, with its genomic features analyzed in comparison with the closely related 'Ca. Phytoplasma' species 'Ca. P. mali' and 'Ca. P. prunorum'. The draft genome spans 456,478 bp with a GC content of 20.4%. Key genes possibly associated with pathogenicity and potential pathogenic effectors were identified, and they are notably lacking orthologs of known effectors. A single potential mobile unit similar to that of 'Ca. P. mali' was identified. It is characterized by the absence of the transposase tra5 and the presence of the IS3 family transposase iSErh1. Multilocus sequence analysis of six genetic markers (16S rRNA gene, LSU36p, tuf, aceF, secA, and secY) from 10 Chilean and 10 Italian samples revealed high genetic uniformity among the Chilean strains, collected from five geographically distant orchards over a span of 13 months; by contrast, strains with greater diversity were detected among those from Italy, collected from a few localities over approximately 30 years. These findings suggest limited evolutionary divergence of this phytoplasma in Chile. This study provides a foundational framework for investigations into the pathogenic mechanisms and evolutionary dynamics of 'Ca. P. pyri'.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1080-1085"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Response of Meloidogyne enterolobii, M. floridensis, M. haplanaria, and M. incognita to Sublethal Doses of Nonfumigant Nematicides. 非熏蒸性杀线虫剂对亚致死剂量的肠绵线虫、佛罗里达绵线虫、haplanaria和incognita的差异反应。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 Epub Date: 2025-08-29 DOI: 10.1094/PHYTO-01-25-0028-R

Nabin Poudel, Richard F Davis, Paul M Severns, Theodore McAvoy, Ganapati B Jagdale, Timothy B Brenneman, Intiaz A Chowdhury

{"title":"Differential Response of Meloidogyne enterolobii, M. floridensis, M. haplanaria, and M. incognita to Sublethal Doses of Nonfumigant Nematicides.","authors":"Nabin Poudel, Richard F Davis, Paul M Severns, Theodore McAvoy, Ganapati B Jagdale, Timothy B Brenneman, Intiaz A Chowdhury","doi":"10.1094/PHYTO-01-25-0028-R","DOIUrl":"10.1094/PHYTO-01-25-0028-R","url":null,"abstract":"Root-knot nematodes (RKNs; Meloidogyne spp.) are among the most widespread and damaging plant-parasitic nematodes known. Although M. incognita is the most prevalent RKN species, other species, including M. enterolobii, M. floridensis, and M. haplanaria, have recently been detected in multiple Georgia, United States, vegetable fields. Producers use chemical nematicides to manage M. incognita populations because most vegetable crops do not have effective RKN host resistance. We evaluated the effects of sublethal doses of nonfumigant nematicides-fluazaindolizine, fluensulfone, fluopyram, and oxamyl-on motility, egg hatching, and reproduction of these four RKN species. In vitro behavioral response assays revealed significant variation in motility between species, with M. enterolobii being the less sensitive to the evaluated nematicides. RKN population response in greenhouse conditions indicated that all the nematicides impaired the reproduction of the evaluated Meloidogyne species. Whereas most nematicides displayed a strong effect on reducing the egg hatching, fluazaindolizine appeared to have a poor effect on suppressing the egg hatching across all RKN species. No differences were observed among Meloidogyne species in their egg-hatching responses to nematicides. In this study, we conducted the first comprehensive evaluation of these nematicides on motility, reproduction, and egg hatching in these RKN species. This study enhances our understanding of the species-specific responses of different Meloidogyne spp. to nonfumigant nematicides.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1215-1222"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PDZ Domain-Containing Protein Prc Is Involved in Virulence and Stress Tolerance in the Poplar Canker Bacterium Lonsdalea populi. 含PDZ结构域蛋白Prc参与杨树溃疡病细菌毒力和胁迫耐受。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 Epub Date: 2025-09-02 DOI: 10.1094/PHYTO-02-25-0066-R

Zexu Ming, Ruirui Yang, Sha Zeng, Aining Li

{"title":"The PDZ Domain-Containing Protein Prc Is Involved in Virulence and Stress Tolerance in the Poplar Canker Bacterium Lonsdalea populi.","authors":"Zexu Ming, Ruirui Yang, Sha Zeng, Aining Li","doi":"10.1094/PHYTO-02-25-0066-R","DOIUrl":"10.1094/PHYTO-02-25-0066-R","url":null,"abstract":"Proteins harboring the PDZ domain are of utmost significance in the infection course of pathogenic bacteria, as well as in the response to external environmental stresses. In this study, we demonstrated that the Lonsdalea populi genome encodes a set of five proteins with the PDZ domain. Through a systematic inactivation of the genes responsible for encoding PDZ proteins, we showed that all these genes are closely related to the virulence of L. populi. Notably, deletion of prc results in suppression of the growth of L. populi and enhanced susceptibility to a diverse array of environmental stressors, such as hydrogen peroxide (H2O2), chloramphenicol, spectinomycin, and metal ions such as Mn2+, Fe2+, and Fe3+. Prc contains four domains: TSPn, PDZ, PEP, and DUF. By separately knocking out the four domains within Prc, we have demonstrated that the TSPn, PDZ, and PEP domains within Prc are all essential components in the pathogenic process of L. populi. Moreover, Prc interacts with the membrane protein YccA. This interaction regulates the biofilm formation capacity and motility of L. populi, thus exerting an impact on its virulence. Together, these findings suggest that Prc is an important regulator of virulence and stress response in L. populi.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1109-1117"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Xanthomonas prunicola Strains Cause Bacterial Leaf Necrosis of Wheat. 新出现的prunicola黄单胞菌引起小麦细菌性叶片坏死。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 DOI: 10.1094/PHYTO-02-25-0044-R

Felipe Clavijo, Verónica Román-Reyna, Rebecca D Curland, Ruth Dill-Macky, Jonathan M Jacobs, Carolina Leoni, Lucía Coimbra, Silvia Pereyra, María I Siri

{"title":"Emerging Xanthomonas prunicola Strains Cause Bacterial Leaf Necrosis of Wheat.","authors":"Felipe Clavijo, Verónica Román-Reyna, Rebecca D Curland, Ruth Dill-Macky, Jonathan M Jacobs, Carolina Leoni, Lucía Coimbra, Silvia Pereyra, María I Siri","doi":"10.1094/PHYTO-02-25-0044-R","DOIUrl":"10.1094/PHYTO-02-25-0044-R","url":null,"abstract":"Xanthomonas prunicola, initially described as a nectarine pathogen, has emerged as the causal agent of a new wheat disease, bacterial leaf necrosis (BLN). This study compared X. prunicola strains isolated from wheat fields in Uruguay and the United States with nectarine-associated strains using genomic and pathogenicity analyses to identify factors driving host specificity. Pathogenicity assays revealed clear differences between the two groups: whereas X. prunicola wheat-associated strains caused distinct dry necrosis lesions in wheat, the reference strain isolated from nectarine failed to induce any symptoms in this host. Surprisingly, most of the wheat strains elicited water-soaking symptoms in Prunus persica, similar to those caused by the X. prunicola strain isolated from nectarine. Complete high-quality genomes were obtained for the six selected X. prunicola strains. Genomic analyses confirmed their identification and revealed that wheat-associated strains form a distinct phylogenomic cluster separated from nectarine-associated strains. Comparative analyses identified key differences, including a functional type VI secretion system, absent in nectarine strains, and unique type III effectors, XopR and XopW, potentially linked to wheat pathogenicity. These findings underscore the importance of understanding the epidemiology of this emerging pathogen, transmission pathways, and host specificity to mitigate its impact on wheat production and develop targeted control strategies.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1097-1108"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium avenaceum Isolates from Pea Are More Aggressive than Those from Wheat: Potential Implications for Pea-Wheat Rotations. 豌豆镰刀菌比小麦镰刀菌更具侵略性：对豌豆-小麦轮作的潜在影响。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 Epub Date: 2025-09-08 DOI: 10.1094/PHYTO-03-25-0120-R

Anas Eranthodi, Teagan I Parkin, Michelle Hubbard, Tom Witte, David Overy, Timothy Schwinghamer, Nora A Foroud, Syama Chatterton

{"title":"Fusarium avenaceum Isolates from Pea Are More Aggressive than Those from Wheat: Potential Implications for Pea-Wheat Rotations.","authors":"Anas Eranthodi, Teagan I Parkin, Michelle Hubbard, Tom Witte, David Overy, Timothy Schwinghamer, Nora A Foroud, Syama Chatterton","doi":"10.1094/PHYTO-03-25-0120-R","DOIUrl":"10.1094/PHYTO-03-25-0120-R","url":null,"abstract":"Fusarium avenaceum is an important root rot pathogen of pulses and a causative agent of Fusarium head blight of cereals. Studies have shown higher abundance of F. avenaceum in the field as a result of pulse-cereal rotations. It is not known whether this rotation can have an influence on the aggressiveness or pathogenicity of the F. avenaceum isolates, as they interact among different host species. In the present work, F. avenaceum isolates collected from common rotation crops, pea, wheat, and lentil, were assessed for root rot in pea and lentil and for head blight in durum wheat. Based on the least squares mean of root rot severity in pea and lentil, the isolate aggressiveness was categorized into low, moderate, and high. The majority of the isolates collected from pea, wheat, and lentil, respectively, were highly aggressive, moderately to highly aggressive, and less aggressive on pea and lentil. In point-inoculated durum wheat, there was no significant difference in aggressiveness between pea- and wheat-derived isolates; a direct comparison with lentil-derived isolates could not be made, as they were screened separately. When comparing the overall isolate aggressiveness, isolates recovered from pea were more aggressive on all three hosts. These data suggest that inclusion of pea in a cereal-based rotation may lead to increased incidence and severity of F. avenaceum-associated head blight in the subsequent wheat crop in the rotation.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1145-1154"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Organic Amendment Composition and Soil Texture in Modulating Volatile Fatty Acids, Fe/Mn Reduction, and Fusarium oxysporum Suppression During Anaerobic Soil Disinfestation in Neutral to Alkaline Soils. 有机改进剂组成和土壤质地在中性至碱性土壤厌氧消毒过程中对挥发性脂肪酸、铁/锰还原和尖孢镰刀菌抑制的调节作用

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 DOI: 10.1094/PHYTO-12-24-0423-R

James Littrell, Bonnie H Ownley, Zachariah R Hansen, Kimberly D Gwinn, David M Butler

{"title":"Role of Organic Amendment Composition and Soil Texture in Modulating Volatile Fatty Acids, Fe/Mn Reduction, and Fusarium oxysporum Suppression During Anaerobic Soil Disinfestation in Neutral to Alkaline Soils.","authors":"James Littrell, Bonnie H Ownley, Zachariah R Hansen, Kimberly D Gwinn, David M Butler","doi":"10.1094/PHYTO-12-24-0423-R","DOIUrl":"10.1094/PHYTO-12-24-0423-R","url":null,"abstract":"Less hazardous alternatives to soil fumigants for suppressing soilborne pathogens, such as pathogenic isolates within the Fusarium oxysporum (Fo) species complex that cause black root rot of strawberry, are urgently needed. A promising alternative is anaerobic soil disinfestation (ASD), in which anaerobic fermentation is induced in soil, leading to production of pathogen-suppressing reduced metal cations (Fe2+, Mn2+) and volatile fatty acids (VFAs) before planting. However, little is known about how interactions between amendment composition, soil texture, and neutral to alkaline soil pH influences Fo suppression via soil biogeochemistry. Suppression of Fo was investigated in soil-based ASD incubation mesocosm trials in which Fo-inoculated soils with initial neutral pH (sand, sandy loam, and silty clay) were amended with lime (0.1% CaCO3) or unamended, and amended (1% wt/wt) with five amendment mixtures of soybean protein isolate and dried molasses (100/0, 75/25, 50/50, 25/75, or 0/100%) for a range of protein/carbohydrate ratios (0.1:1 to 32:1) to initiate ASD treatments. Post-ASD, soil chemical properties were measured, including VFA and Fe2+/Mn2+ concentrations, and Fo inoculum viability was assessed. Total soil VFA and Fe2+/Mn2+ concentrations were influenced by ASD amendment composition and soil texture, with the highest VFA concentrations resulting from high protein amendments and the highest Mn2+ concentrations observed in sandy loam and silty clay soils. Fo viability was reduced in all amended treatments, but finer soil textures were associated with lower Fo suppression. Our results highlight the importance of soil texture and amendment composition in influencing ASD effectiveness in neutral to alkaline soils.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1134-1144"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Mycotoxin and Furanoterpenoid Production by Fusarium Species Infecting Sweetpotato. 甘薯镰刀菌产生真菌毒素和呋喃萜类的特性研究。

IF 3.1 2区农林科学

Phytopathology Pub Date : 2025-09-01 Epub Date: 2025-09-09 DOI: 10.1094/PHYTO-01-25-0031-R

U Bhatta, J R Standish, O Baars, L M Quesada-Ocampo

{"title":"Characterization of Mycotoxin and Furanoterpenoid Production by Fusarium Species Infecting Sweetpotato.","authors":"U Bhatta, J R Standish, O Baars, L M Quesada-Ocampo","doi":"10.1094/PHYTO-01-25-0031-R","DOIUrl":"10.1094/PHYTO-01-25-0031-R","url":null,"abstract":"Sweetpotato (Ipomoea batatas) production is threatened by Fusarium root rot, which can lead to substantial yield losses and contamination with mycotoxins and phytotoxins. This study investigated the production of mycotoxins by different Fusarium species and isolates in vitro and in vivo, as well as furanoterpenoid compounds produced by sweetpotato in response to Fusarium infection and wounding. Among 37 Fusarium isolates tested, only four F. proliferatum isolates (AS050, AS116, JS603, and NM229) produced significant levels of fumonisins B1 and B2 in sweetpotato roots, with NM229 exhibiting the highest mycotoxin concentrations. Notably, fumonisin production differed between in vivo and in vitro conditions. In sweetpotato roots, fumonisin concentrations for NM229 decreased over time, whereas in liquid culture, concentrations increased. The other isolates produced minimal amounts of fumonisins, with no significant changes over time in either condition. An untargeted metabolomics analysis showed several putative furanoterpenoids, both wounding-induced and Fusarium-specific. Wounding alone triggered production of certain furanoterpenoids, whereas Fusarium infections, particularly F. denticulatum infections, resulted in the highest levels of phytotoxins, including ipomeamarone, 4-hydroxymyoporone, 1-ipomeanol, and 4-ipomeanol. F. acuminatum, F. commune, and F. proliferatum isolates induced some additional putative furanoterpenoids based on observed similar fragmentation patterns. These findings highlight the complex interaction between mechanical damage and Fusarium infection in stimulating phytotoxin production in sweetpotato and emphasize the importance of effective disease management strategies. The detection of high fumonisin levels, exceeding FDA guidelines for human food (2 to 4 ppm), underscores the need for monitoring and controlling Fusarium infections in sweetpotato production.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"1204-1214"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0