Phytopathology最新文献_第4页

Comparative Transcriptomic Analysis of Soybean Cyst Nematode Inbred Populations Non-adapted or Adapted on Soybean rhg1-a/Rhg4-Mediated Resistance. 不适应或适应大豆 rhg1-a/Rhg4 介导的抗性的大豆胞囊线虫近交系种群的转录组比较分析。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-04 DOI: 10.1094/PHYTO-03-24-0095-R

Khee Man Kwon, Rick E Masonbrink, Thomas R Maier, Michael N Gardner, Andrew J Severin, Thomas J Baum, Melissa G Mitchum

{"title":"Comparative Transcriptomic Analysis of Soybean Cyst Nematode Inbred Populations Non-adapted or Adapted on Soybean rhg1-a/Rhg4-Mediated Resistance.","authors":"Khee Man Kwon, Rick E Masonbrink, Thomas R Maier, Michael N Gardner, Andrew J Severin, Thomas J Baum, Melissa G Mitchum","doi":"10.1094/PHYTO-03-24-0095-R","DOIUrl":"10.1094/PHYTO-03-24-0095-R","url":null,"abstract":"Soybean cyst nematode (SCN, Heterodera glycines) is most effectively managed through planting resistant soybean cultivars, but the repeated use of the same resistance sources has led to a widespread emergence of virulent SCN populations that can overcome soybean resistance. Resistance to SCN HG type 0 (Race 3) in soybean cultivar Forrest is mediated by an epistatic interaction between the soybean resistance genes rhg1-a and Rhg4. We previously developed two SCN inbred populations by mass-selecting SCN HG type 0 (Race 3) on susceptible and resistant recombinant inbred lines, derived from a cross between Forrest and the SCN-susceptible cultivar Essex, which differ for Rhg4. To identify SCN genes potentially involved in overcoming rhg1-a/Rhg4-mediated resistance, we conducted RNA sequencing on early parasitic juveniles of these two SCN inbred populations infecting their respective hosts, only to discover a handful of differentially expressed genes (DEGs). However, in a comparison with early parasitic juveniles of an avirulent SCN inbred population infecting a resistant host, we discovered 59 and 171 DEGs uniquely up- or downregulated in virulent parasitic juveniles adapted on the resistant host. Interestingly, the proteins coded by these 59 DEGs included vitamin B-associated proteins (reduced folate carrier, biotin synthase, and thiamine transporter) and nematode effectors known to play roles in plant defense suppression, suggesting that virulent SCN may exert a heightened transcriptional response to cope with enhanced plant defenses and an altered nutritional status of a resistant soybean host. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2341-2350"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of Increasing Soil Salinity on Genetic Resistance (I-3 Gene)-Based Management of Fusarium Wilt (Fusarium oxysporum f. sp. lycopercisi Race 3) in California Processing Tomatoes. 土壤盐度增加对基于遗传抗性（I-3 基因）的加利福尼亚加工番茄镰刀菌枯萎病（F. oxysporum f. sp. lycopercisi race 3）管理的影响。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-03 DOI: 10.1094/PHYTO-10-23-0402-KC

Elizabeth M Hellman, Thomas Turini, Cassandra L Swett

{"title":"Impacts of Increasing Soil Salinity on Genetic Resistance (I-3 Gene)-Based Management of Fusarium Wilt (Fusarium oxysporum f. sp. lycopercisi Race 3) in California Processing Tomatoes.","authors":"Elizabeth M Hellman, Thomas Turini, Cassandra L Swett","doi":"10.1094/PHYTO-10-23-0402-KC","DOIUrl":"10.1094/PHYTO-10-23-0402-KC","url":null,"abstract":"California is the primary processing tomato (Solanum lycopersicum) producer in the United States. Fusarium oxysporum f. sp. lycopercisi race 3 (Fol3), the cause of Fusarium wilt, is a major driver of yield losses. Fol3 has recently been observed causing disease in resistant cultivars (I-3 R-gene), often reported in association with high soil salinity. This study was undertaken to better understand the role of salinity in compromising resistance-based management of Fol3. Surveys established opportunity for salinity-Fol3-tomato interactions in 44% of commercial fields examined, with harmful soil salt levels up to 3.6 dS/m (P < 0.001), high sodium (P < 0.001), and high sodicity (sodium adsorption ratio > 13; P < 0.001). In controlled field studies of Fol3 in NaCl/CaCl2-treated soil, Fol3-resistant cultivars either only developed wilt under salt or only developed wilt above the industry non-hybrid threshold (2%) under salt across two trial years. The absence of yield differences indicates low to no economic impact of disease enhancement (P > 0.05). NaCl, CaCl2, and Na2SO4 had no effect on Fol3 propagule production in liquid agar versus water agar controls (P > 0.05), although CaCl2 increased propagule loads sevenfold versus ionic controls (polyethylene glycol) (P = 0.036). NaCl/CaCl2 (2:1) reduced propagule loads up to 65% versus no salt (P = 0.029) in soil with pathogen-infested tomato tissue. These results together establish the opportunity for salinity-Fol3-tomato interactions and potential for salt to influence the efficacy of resistant cultivar-based management-this does not appear to be primarily due to salt enhancement of pathogen populations, pointing to a yet-unexplored direct influence of salt on host resistance.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2252-2261"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What Explains Hop Growers' Fungicide Use Intensity and Management Costs in Response to Powdery Mildew? 酒花种植者在应对白粉病时使用杀菌剂的强度和管理成本的原因是什么？

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-15 DOI: 10.1094/PHYTO-04-24-0127-R

Jae Young Hwang, Sharmodeep Bhattacharyya, Shirshendu Chatterjee, Thomas L Marsh, Joshua F Pedro, David H Gent

{"title":"What Explains Hop Growers' Fungicide Use Intensity and Management Costs in Response to Powdery Mildew?","authors":"Jae Young Hwang, Sharmodeep Bhattacharyya, Shirshendu Chatterjee, Thomas L Marsh, Joshua F Pedro, David H Gent","doi":"10.1094/PHYTO-04-24-0127-R","DOIUrl":"10.1094/PHYTO-04-24-0127-R","url":null,"abstract":"Methods for causal inference from observational data are common in human disease epidemiology and social sciences but are used relatively little in plant pathology. We draw upon an extensive data set of the incidence of hop plants with powdery mildew (caused by Podosphaera macularis) collected from yards in Oregon from 2014 to 2017 and associated metadata on grower cultural practices, cultivar susceptibility to powdery mildew, and pesticide application records to understand variation in and causes of growers' fungicide use and associated costs. An instrumental causal forest model identified growers' spring pruning thoroughness, cultivar susceptibility to two of the dominant pathogenic races of P. macularis, network centrality of yards during May-June and June-July time transitions, and the initial strain of the fungus detected as important variables determining the number of pesticide active constituents applied by growers and the associated costs they incurred in response to powdery mildew. Exposure-response function models fit after covariate weighting indicated that both the number of pesticide active constituents applied and their associated costs scaled linearly with the seasonal mean incidence of plants with powdery mildew. Although the causes of pesticide use intensity are multifaceted, biological and production factors collectively influence the incidence of powdery mildew, which has a direct exposure-response relationship with the number of pesticide active constituents that growers apply and their costs. Our analyses point to several potential strategies for reducing pesticide use and costs for management of powdery mildew on hop. We also highlight the utility of these methods for causal inference in observational studies.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2287-2299"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141910035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bursaphelenchus xylophilus Venom Allergen-Like Protein BxVAP1, Triggering Plant Defense-Related Programmed Cell Death, Plays an Important Role in Regulating Pinus massoniana Terpene Defense Responses. Bursaphelenchus xylophilus 毒液过敏原样蛋白 BxVAP1 触发植物防御相关的程序性细胞死亡，在调控 Pinus massoniana 萜烯防御反应中发挥重要作用。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-09-30 DOI: 10.1094/PHYTO-01-24-0026-R

Yuqian Feng, Yongxia Li, Zhenkai Liu, Xuan Wang, Wei Zhang, Dongzhen Li, Xiaojian Wen, Xingyao Zhang

{"title":"Bursaphelenchus xylophilus Venom Allergen-Like Protein BxVAP1, Triggering Plant Defense-Related Programmed Cell Death, Plays an Important Role in Regulating Pinus massoniana Terpene Defense Responses.","authors":"Yuqian Feng, Yongxia Li, Zhenkai Liu, Xuan Wang, Wei Zhang, Dongzhen Li, Xiaojian Wen, Xingyao Zhang","doi":"10.1094/PHYTO-01-24-0026-R","DOIUrl":"10.1094/PHYTO-01-24-0026-R","url":null,"abstract":"Bursaphelenchus xylophilus (pine wood nematode, PWN), a migratory plant-parasitic nematode, acts as an etiological agent, inflicting considerable damage to pine forests worldwide. Plant immunity constitutes a crucial factor in resisting various pathogenic invasions. The primary defensive responses of host pines against PWN infection encompass terpene accumulation, defense response-related gene expression, and programmed cell death. Venom allergen-like proteins (VAPs), as potential effectors, are instrumental in facilitating the successful colonization of PWNs. In this study, we investigated the inhibition of B. xylophilus VAP (BxVAP1) expression by RNA interference in vitro. Following BxVAP1 silencing, the reproduction rate and migration rate of the PWN population in Pinus massoniana decreased, the expression of the α-pinene synthase gene was induced, other terpene synthase and pathogenesis-related genes were inhibited and delayed, the peak times and levels of terpene-related substances were changed, and the degree of cavitation in P. massoniana was diminished. Transient expression of BxVAP1 in Nicotiana benthamiana revealed that BxVAP1 was expressed in both the cell membrane and nucleus, inducing programmed cell death and the expression of pathogen-associated molecular pattern-triggered immunity marker genes (NbAcre31 and NbPTI5). This study is the first to demonstrate that silencing the BxVAP1 gene affects host defense responses, including terpenoid metabolism in P. massoniana, and that BxVAP1 can be recognized by N. benthamiana as an effector to trigger its innate immunity, expanding our understanding of the parasitic mechanism of B. xylophilus.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2331-2340"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Initial Inoculum on the Temporal and Spatial Dynamics of Wheat Blast Under Field Conditions in Bolivia. 玻利维亚田间条件下初始接种量对小麦稻瘟病时空动态的影响。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-03 DOI: 10.1094/PHYTO-12-23-0491-R

Carlos C Góngora-Canul, Alexandria Volkening, Jorge Cuéllar, Lidia Calderón, Mariela Fernández-Campos, Da Young Lee, Jorge Salgado, Andres Cruz-Sancan, C D Cruz

{"title":"Effect of Initial Inoculum on the Temporal and Spatial Dynamics of Wheat Blast Under Field Conditions in Bolivia.","authors":"Carlos C Góngora-Canul, Alexandria Volkening, Jorge Cuéllar, Lidia Calderón, Mariela Fernández-Campos, Da Young Lee, Jorge Salgado, Andres Cruz-Sancan, C D Cruz","doi":"10.1094/PHYTO-12-23-0491-R","DOIUrl":"10.1094/PHYTO-12-23-0491-R","url":null,"abstract":"Epidemiological studies to better understand wheat blast (WB) spatial and temporal patterns were conducted in three field environments in Bolivia between 2019 and 2020. The temporal dynamics of wheat leaf blast (WLB) and spike blast (WSB) were best described by the logistic model compared with the Gompertz and exponential models. The nonlinear logistic infection rates were higher under defined inoculation in experiments two and three than under undefined inoculation in experiment one, and they were also higher for WSB than for WLB. The onset of WLB began with a spatial clustering pattern according to autocorrelation analysis and Moran's index values, with higher severity and earlier onset for defined than for undefined inoculation until the last sampling time. The WSB onset did not start with a spatial clustering pattern; instead, it was detected later until the last sampling date across experiments, with higher severity and earlier onset for defined than for undefined inoculation. Maximum severity (Kmax) was 1.0 for WSB and less than 1.0 for WLB. Aggregation of WLB and WSB was higher for defined than for undefined inoculation. The directionality of hotspot development was similar for both WLB and WSB, mainly occurring concentrically for defined inoculation. Our results show no evidence of synchronized development but suggest a temporal and spatial progression of disease symptoms on wheat leaves and spikes. Thus, we recommend that monitoring and management of WB should be considered during early growth stages of wheat planted in areas of high risk.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2273-2286"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas syringae pv. actinidiae Unique Effector HopZ5 Interacts with GF14C to Trigger Plant Immunity. Pseudomonas syringae pv. actinidiae 独特的效应因子 HopZ5 与 GF14C 相互作用，触发植物免疫。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-04 DOI: 10.1094/PHYTO-09-23-0330-R

Mingxia Zhou, Jinglong Zhang, Zhibo Zhao, Wei Liu, Zhiran Wu, Lili Huang

{"title":"Pseudomonas syringae pv. actinidiae Unique Effector HopZ5 Interacts with GF14C to Trigger Plant Immunity.","authors":"Mingxia Zhou, Jinglong Zhang, Zhibo Zhao, Wei Liu, Zhiran Wu, Lili Huang","doi":"10.1094/PHYTO-09-23-0330-R","DOIUrl":"10.1094/PHYTO-09-23-0330-R","url":null,"abstract":"The bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) is the most devastating disease threatening the global kiwifruit production. This pathogen delivers multiple effector proteins into plant cells to resist plant immune responses and facilitate their survival. Here, we focused on the unique effector HopZ5 in Psa, which previously has been reported to have virulence functions. In this study, our results showed that HopZ5 could cause macroscopic cell death and trigger a serious immune response by agroinfiltration in Nicotiana benthamiana, along with upregulated expression of immunity-related genes and significant accumulation of reactive oxygen species and callose. Subsequently, we confirmed that HopZ5 interacted with the phosphoserine-binding protein GF14C in both the nonhost plant N. benthamiana (NbGF14C) and the host plant kiwifruit (AcGF14C), and silencing of NbGF14C compromised HopZ5-mediated cell death, suggesting that GF14C plays a crucial role in the detection of HopZ5. Further studies showed that overexpression of NbGF14C both markedly reduced the infection of Sclerotinia sclerotiorum and Phytophthora capsica in N. benthamiana, and overexpression of AcGF14C significantly enhanced the resistance of kiwifruit against Psa, indicating that GF14C positively regulates plant immunity. Collectively, our results revealed that the virulence effector HopZ5 could be recognized by plants and interact with GF14C to activate plant immunity.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2322-2330"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness and Genetic Control of Trichoderma spp. as a Biological Control of Wheat Powdery Mildew Disease. 毛霉菌作为小麦白粉病生物防治的有效性和遗传控制。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-16 DOI: 10.1094/PHYTO-05-24-0157-R

Amira M I Mourad, Andreas Börner, Samar M Esmail

{"title":"Effectiveness and Genetic Control of Trichoderma spp. as a Biological Control of Wheat Powdery Mildew Disease.","authors":"Amira M I Mourad, Andreas Börner, Samar M Esmail","doi":"10.1094/PHYTO-05-24-0157-R","DOIUrl":"10.1094/PHYTO-05-24-0157-R","url":null,"abstract":"Wheat powdery mildew (WPM) is one of the most devasting diseases that affects wheat yield worldwide. Few efforts have been made to control such a serious disease. An effective way to control WPM is urgently needed. Biological control is an effective way to control plant diseases worldwide. In this study, the efficiency of three different Trichoderma spp. in controlling WPM at the seedling growth stage was tested using 35 highly diverse wheat genotypes. Highly significant differences were found in WPM resistance among the four treatments, confirming the efficiency of Trichoderma in controlling WPM. Of the three species, T. asperellum T34 (T34) was the most effective species in controlling WPM, as it reduced the symptoms by 50.56%. A set of 196 wheat genotypes was used to identify the genetic control of the WPM resistance induced by T34. A total of 39, 27, and 18 gene models were identified to contain the significant markers under Pm, T34, and the improvement in powdery mildew resistance due to T34 (T34_improvement) conditions. Furthermore, no gene model was common between T34 and Pm, suggesting the presence of completely different genetic systems controlling the resistance under T34 and Pm. The functional annotation and biological process pathways of the detected gene models confirm their association with the normal and induced resistance. This study, for the first time, confirms the efficiency of T34 in controlling WPM and provides a deep understanding of the genetic control of induced and normal resistance to WPM.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2221-2234"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining Single-Gene-Resistant and Pyramided Cultivars of Perennial Crops in Agricultural Landscapes Compromises Pyramiding Benefits in Most Production Situations. 在农业景观中将多年生作物的单基因抗性品种与金字塔型栽培品种结合起来，在大多数生产情况下都会损害金字塔型栽培的效益。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-15 DOI: 10.1094/PHYTO-02-24-0075-R

Marta Zaffaroni, Julien Papaïx, Abebayehu G Geffersa, Jean-François Rey, Loup Rimbaud, Frédéric Fabre

{"title":"Combining Single-Gene-Resistant and Pyramided Cultivars of Perennial Crops in Agricultural Landscapes Compromises Pyramiding Benefits in Most Production Situations.","authors":"Marta Zaffaroni, Julien Papaïx, Abebayehu G Geffersa, Jean-François Rey, Loup Rimbaud, Frédéric Fabre","doi":"10.1094/PHYTO-02-24-0075-R","DOIUrl":"10.1094/PHYTO-02-24-0075-R","url":null,"abstract":"Although resistant cultivars are valuable in safeguarding crops against diseases, they can be rapidly overcome by pathogens. Numerous strategies have been proposed to delay pathogen adaptation (evolutionary control) while still ensuring effective protection (epidemiological control). For perennial crops, multiple resistance genes can be deployed (i) in the same cultivar (pyramiding strategy); in single-gene-resistant cultivars grown (ii) in the same field (mixture strategy) or (iii) in different fields (mosaic strategy); or (iv) in hybrid strategies that combine the three previous options. In addition, the spatial scale at which resistant cultivars are deployed can affect the plant-pathogen interaction: Small fields are thought to reduce pest density and disease transmission. Here, we used the spatially explicit stochastic model landsepi to compare the evolutionary and epidemiological control across spatial scales and deployment strategies relying on two major resistance genes. Our results, broadly focused on resistance to downy mildew of grapevine, show that the evolutionary control provided by the pyramiding strategy is at risk when single-gene-resistant cultivars are concurrently planted in the landscape (hybrid strategies), especially at low mutation probability. Moreover, the effectiveness of pyramiding compared with hybrid strategies is influenced by whether the adapted pathogen pays a fitness cost across all hosts or only for unnecessary virulence, particularly when the fitness cost is high rather than intermediate. Finally, field size did not affect model outputs for a wide range of mutation probabilities and associated fitness costs. The socioeconomic policies favoring the adoption of optimal resistant management strategies are discussed.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2310-2321"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Resequencing Reveals Significant Genetic Differentiation Between Exserohilum turcicum Populations from Maize and Sorghum and Candidate Effector Genes Related to Host Specificity. 全基因组重测序揭示了来自玉米和高粱的 Exserohilum turcicum 群体之间的显著遗传分化，以及与宿主特异性相关的候选效应基因。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-07 DOI: 10.1094/PHYTO-05-24-0172-R

Linkai Cui, Cong Wang, Mengqi Li, Yufeng Fang, Yanhong Hu

{"title":"Whole-Genome Resequencing Reveals Significant Genetic Differentiation Between Exserohilum turcicum Populations from Maize and Sorghum and Candidate Effector Genes Related to Host Specificity.","authors":"Linkai Cui, Cong Wang, Mengqi Li, Yufeng Fang, Yanhong Hu","doi":"10.1094/PHYTO-05-24-0172-R","DOIUrl":"10.1094/PHYTO-05-24-0172-R","url":null,"abstract":"Exserohilum turcicum is a devastating fungal pathogen that infects both maize and sorghum, leading to severe leaf diseases of the two crops. According to host specificity, pathogenic isolates of E. turcicum are divided into two formae speciales, namely E. turcicum f. sp. zeae and E. turcicum f. sp. sorghi. To date, the molecular mechanism underlying the host specificity of E. turcicum is marginally known. In this study, the whole genomes of 60 E. turcicum isolates collected from both maize and sorghum were resequenced, which enabled identification of 233,022 single-nucleotide polymorphisms (SNPs) in total. Phylogenetic analysis indicated that all isolates are clustered into four genetic groups that have a close relationship with host source. This observation is validated by the result of principal component analysis. Analysis of population structure revealed that there is obvious genetic differentiation between two populations from maize and sorghum. Further analysis showed that 5,431 SNPs, including 612 nonsynonymous SNPs, are completely co-segregated with the host source. These nonsynonymous SNPs are located in 539 genes, among which 18 genes are predicted to encode secretory proteins, including six putative effector genes named SIX13-like, Ecp6, GH12, GH28-1, GH28-2, and CHP1. Sequence polymorphism analysis revealed various numbers of SNPs in the coding regions of these genes. These findings provide new insights into the molecular basis of host specificity in E. turcicum.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2351-2359"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodopseudomonas palustris Atp2 Protein Exerts Antifungal Effects by Targeting the Ribosomal Protein MoRpl12 in Magnaporthe oryzae. 黄绿假单胞菌 Atp2 蛋白通过靶向 Magnaporthe oryzae 的核糖体蛋白 MoRpl12 发挥抗真菌作用

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 DOI: 10.1094/PHYTO-05-24-0169-R

Chunyan Chen, Xiyang Wu, Qiang Huang, Yingfei Qin, Chenggang Li, Xin Zhang, Pei Wang, Xinqiu Tan, Yong Liu, Yue Chen, Deyong Zhang

{"title":"Rhodopseudomonas palustris Atp2 Protein Exerts Antifungal Effects by Targeting the Ribosomal Protein MoRpl12 in Magnaporthe oryzae.","authors":"Chunyan Chen, Xiyang Wu, Qiang Huang, Yingfei Qin, Chenggang Li, Xin Zhang, Pei Wang, Xinqiu Tan, Yong Liu, Yue Chen, Deyong Zhang","doi":"10.1094/PHYTO-05-24-0169-R","DOIUrl":"10.1094/PHYTO-05-24-0169-R","url":null,"abstract":"Rice blast is one of the most hazardous diseases affecting rice production. Previously, we discovered that the Atp2 protein of Rhodopseudomonas palustris could significantly inhibit the appressorium formation and pathogenicity of Magnaporthe oryzae. However, the molecular mechanism of this fungus has remained unknown. This study revealed that Atp2 can enter the cell and interact with the ribosomal protein MoRpl12 of M. oryzae, directly affecting the expression of the MoRpl12 protein. Silencing the MoRPL12 gene can affect cell wall integrity, growth, conidiogenesis, and fungal pathogenicity. The quantitative reverse transcription PCR results showed significant changes in the expression of conidiation-related genes in the MoRPL12 gene-silenced mutants or in the Atp2 protein-treated plants. We further found that Atp2 treatment can influence the expression of ribosomal-related genes, such as RPL, in M. oryzae. Our study revealed a novel antifungal mechanism by which the Atp2 protein binds to the ribosomal protein MoRpl12 and inhibits the pathogenicity of rice blast fungus, providing a new potential target for rice blast prevention and control.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2235-2243"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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