{"title":"NIb相互作用位点3597AUU和3607GGCU对小麦黄花叶病毒RNA2的复制至关重要。","authors":"Guowei Geng, Minjun Liu, Xueyuan Wang, Xuefeng Yuan","doi":"10.1094/PHYTO-05-25-0158-SC","DOIUrl":null,"url":null,"abstract":"<p><p>Wheat yellow mosaic virus (WYMV) is the main cause of wheat yellow mosaic disease. Although its regulation of protein translation and interactions with host proteins are well-studied, independent regulation of the virus genome is poorly understood. This study performed in vitro experiments investigating replication regulation by the 5' UTR and 3' UTR of WYMV RNA2. The results confirm that the RNA-dependent RNA polymerase (NIb) can only recognize and catalyze the synthesis of 3' 190 nt complementary chains in vitro. RNA structure probing and mutation analysis identified <sup>3597</sup>AUU and <sup>3607</sup>GGCU as novel interaction sites of NIb; both are essential for maintaining normal replication. Our findings provide an empirical basis for in vivo experiments on regulation of WYMV genome replication and provide theoretical basis for the prevention and control of WYMV-related crop diseases.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NIb Interaction Sites <sup>3597</sup>AUU and <sup>3607</sup>GGCU Are Crucial to the Replication of Wheat Yellow Mosaic Virus RNA2.\",\"authors\":\"Guowei Geng, Minjun Liu, Xueyuan Wang, Xuefeng Yuan\",\"doi\":\"10.1094/PHYTO-05-25-0158-SC\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Wheat yellow mosaic virus (WYMV) is the main cause of wheat yellow mosaic disease. Although its regulation of protein translation and interactions with host proteins are well-studied, independent regulation of the virus genome is poorly understood. This study performed in vitro experiments investigating replication regulation by the 5' UTR and 3' UTR of WYMV RNA2. The results confirm that the RNA-dependent RNA polymerase (NIb) can only recognize and catalyze the synthesis of 3' 190 nt complementary chains in vitro. RNA structure probing and mutation analysis identified <sup>3597</sup>AUU and <sup>3607</sup>GGCU as novel interaction sites of NIb; both are essential for maintaining normal replication. Our findings provide an empirical basis for in vivo experiments on regulation of WYMV genome replication and provide theoretical basis for the prevention and control of WYMV-related crop diseases.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-05-25-0158-SC\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-05-25-0158-SC","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
NIb Interaction Sites 3597AUU and 3607GGCU Are Crucial to the Replication of Wheat Yellow Mosaic Virus RNA2.
Wheat yellow mosaic virus (WYMV) is the main cause of wheat yellow mosaic disease. Although its regulation of protein translation and interactions with host proteins are well-studied, independent regulation of the virus genome is poorly understood. This study performed in vitro experiments investigating replication regulation by the 5' UTR and 3' UTR of WYMV RNA2. The results confirm that the RNA-dependent RNA polymerase (NIb) can only recognize and catalyze the synthesis of 3' 190 nt complementary chains in vitro. RNA structure probing and mutation analysis identified 3597AUU and 3607GGCU as novel interaction sites of NIb; both are essential for maintaining normal replication. Our findings provide an empirical basis for in vivo experiments on regulation of WYMV genome replication and provide theoretical basis for the prevention and control of WYMV-related crop diseases.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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