Phytopathology最新文献_第5页

Validation of Fungicide Spray Strategies and Selection for Fenhexamid Resistance in Botrytis cinerea on Greenhouse-Grown Grapevines. 在温室栽培的葡萄藤上验证喷洒杀真菌剂的策略，并筛选出对芬克菌胺具有抗性的葡萄灰霉病菌。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-09-13 DOI: 10.1094/PHYTO-11-23-0443-R

Stephen C Boushell, Mengjun Hu

引用次数: 0

Effects of Different Light Conditions on Anatomical and Histological Features of Galls in Bacterial Gall Disease of Cerasus × yedoensis. 不同光照条件对 Cerasus × yedoensis 细菌性瘿病虫瘿解剖学和组织学特征的影响。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-14 DOI: 10.1094/PHYTO-06-22-0221-R

Takefumi Ikeda, Misaki Okuda, Makoto Ishihara, Yasuo Kon-No

{"title":"Effects of Different Light Conditions on Anatomical and Histological Features of Galls in Bacterial Gall Disease of Cerasus × yedoensis.","authors":"Takefumi Ikeda, Misaki Okuda, Makoto Ishihara, Yasuo Kon-No","doi":"10.1094/PHYTO-06-22-0221-R","DOIUrl":"10.1094/PHYTO-06-22-0221-R","url":null,"abstract":"Cerasus × yedoensis (cherry 'Somei-yoshino' Fujino) is affected by bacterial gall disease caused by Pseudomonas syringae pv. cerasicola (PSC). C. × yedoensis is often infected with PSC under weak light intensity, which indicates that susceptibility of C. × yedoensis to PSC is affected by light. To evaluate the effects of white light intensity and different light qualities, white or blue, on bacterial gall disease development, we quantitatively assessed the anatomical and histological features of bacterial-inoculated sites on branches of 2-year-old potted C. × yedoensis seedlings grown under different light intensities and qualities. The stronger the white light intensity, the less severe the gall symptoms. Gall formation was suppressed more by blue than white light of the same intensity. The validity of a simple gall index for assessing gall development with the naked eye, via quantitative evaluation of gall shape by measuring gall height, width, and volume, showed that the gall index could be used as a practical method for on-site assessments of gall development. The ratio of degeneration area in the gall remained constant, suggesting the presence of some regulatory mechanism preventing PSC from affecting the entire gall within the plant. Microscopy showed that the gall tissue is composed primarily of callus cells and has voids containing gummy material that is exuded from cracks in the gall, and the periderm develops at the gall foot but not at the gall apex, so the cells at the gall apex were necrotic or collapsed.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2196-2206"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-Driven Selection, Revealed by Comparative Analysis of Xanthomonas Type III Secretion Effectoromes, Unveils Novel Recognized Effectors. 通过比较分析黄单胞菌 III 型分泌效应器基因组发现的宿主驱动选择揭示了新型识别效应器。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-16 DOI: 10.1094/PHYTO-04-24-0147-R

Yao Xiao, Shatrupa Ray, Saul Burdman, Doron Teper

{"title":"Host-Driven Selection, Revealed by Comparative Analysis of Xanthomonas Type III Secretion Effectoromes, Unveils Novel Recognized Effectors.","authors":"Yao Xiao, Shatrupa Ray, Saul Burdman, Doron Teper","doi":"10.1094/PHYTO-04-24-0147-R","DOIUrl":"10.1094/PHYTO-04-24-0147-R","url":null,"abstract":"Xanthomonas species are specialized plant pathogens, often exhibiting a narrow host range. They rely on the translocation of effector proteins through the type III secretion system to colonize their respective hosts. The effector arsenal varies among Xanthomonas spp., typically displaying species-specific compositions. This species-specific effector composition, collectively termed the effectorome, is thought to influence host specialization. We determined the plant host-derived effectoromes of more than 300 deposited genomes of Xanthomonas species associated with either Solanaceae or Brassicaceae hosts. Comparative analyses revealed clear species-specific effectorome signatures. However, Solanaceae or Brassicaceae host-associated effectorome signatures were not detected. Nevertheless, host biases in the presence or absence of specific effector classes were observed. To assess whether host-associated effector absence results from selective pressures, we introduced effectors unique to Solanaceae pathogens to X. campestris pv. campestris and effectors unique to Brassicaceae pathogens to X. euvesicatoria pv. euvesicatoria (Xeue) and evaluated if these introductions hindered virulence on their respective hosts. Introducing the effector XopI into X. campestris pv. campestris reduced virulence on white cabbage leaves without affecting localized or systemic colonization. Introducing the XopAC or XopJ5 effectors into Xeue reduced virulence and colonization on tomato but not on pepper. Additionally, XopAC and XopJ5 induced a hypersensitive response on tomato leaves when delivered by Xeue or through Agrobacterium-mediated transient expression, confirming recognition in tomato. This study demonstrates the role of host-derived selection in establishing species-specific effectoromes, identifying XopAC and XopJ5 as recognized effectors in tomato.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2207-2220"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Characterization of the Histone Acetyltransferase FcElp3 in Lotus Rhizome Rot-Causing Fungus Fusarium commune. 荷花根茎腐烂病致病真菌 Fusarium commune 中组蛋白乙酰转移酶 FcElp3 的功能特征。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-01 Epub Date: 2024-10-04 DOI: 10.1094/PHYTO-01-24-0017-R

Lifang Ye, Weigang Kuang, Lianhu Zhang, Yachun Lin, Yifan Zhang, Xiaotang Sun, Ruqiang Cui

{"title":"Functional Characterization of the Histone Acetyltransferase FcElp3 in Lotus Rhizome Rot-Causing Fungus Fusarium commune.","authors":"Lifang Ye, Weigang Kuang, Lianhu Zhang, Yachun Lin, Yifan Zhang, Xiaotang Sun, Ruqiang Cui","doi":"10.1094/PHYTO-01-24-0017-R","DOIUrl":"10.1094/PHYTO-01-24-0017-R","url":null,"abstract":"Fusarium commune is the main pathogen of lotus rhizome rot, which causes the wilt of many plants. Histone acetyltransferase plays a critical part in the growth and virulence of fungi. In the present study, we identified an FcElp3 in F. commune homologous to histone acetyltransferase Elp3. We further constructed a mutant strain of F. commune to determine the function of FcElp3 in fungal growth and pathogenicity. The results showed that the deletion of FcElp3 resulted in reduced mycelial growth and sporulation. Compared with the wild type, the ΔFcElp3 strain showed more tolerance to osmotic stress and cell wall stress responses but was highly sensitive to oxidative stress. The subcellular localization results indicated that FcElp3 was distributed in both the cytoplasm and nucleus. Western blotting showed that FcElp3 was important for acetylation of H3K14 and H4K8. RNA sequencing analysis showed significant transcriptional changes in the ΔFcElp3 mutant, with 3,098 genes upregulated and 5,770 genes downregulated. Peroxisome was the most significantly enriched metabolic pathway for downregulated genes. This led to a significant decrease in the expression of the core transcription factor Fcap1 involved in the oxidative stress response. Pathogenicity tests revealed that the ΔFcElp3 mutant's pathogenicity on lotus was significantly decreased. Together, these findings clearly demonstrated that FcElp3 was involved in fungal growth, development, stress response, and pathogenicity via the direct regulation of multiple target genes.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2300-2309"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of loop-mediated isothermal amplification (LAMP) in plant pathogen detection. 环介导等温扩增（LAMP）在植物病原体检测中的应用。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-09-30 DOI: 10.1094/PHYTO-10-23-0391-KC

Liuliu Yang, Yu Sun, Lin Sun, Zehao Wang, Jie Feng, Yue Liang

引用次数: 0

Transient Expression of Nicotiana tabacum Silicon-Induced Histidine-Rich Defensins in Nicotiana benthamiana Limits Necrotic Lesion Development Caused by Phytopathogenic Fungi. 在烟草根中瞬时表达烟草硅诱导的富组氨酸防御素，可限制由植物病原真菌引起的坏死性病变的发展。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-09-30 DOI: 10.1094/PHYTO-05-24-0162-R

Stephen Muhindi, Wendy Zellner, Shin-Yi Marzano, Jennifer Boldt, Scott Leisner

{"title":"Transient Expression of Nicotiana tabacum Silicon-Induced Histidine-Rich Defensins in Nicotiana benthamiana Limits Necrotic Lesion Development Caused by Phytopathogenic Fungi.","authors":"Stephen Muhindi, Wendy Zellner, Shin-Yi Marzano, Jennifer Boldt, Scott Leisner","doi":"10.1094/PHYTO-05-24-0162-R","DOIUrl":"https://doi.org/10.1094/PHYTO-05-24-0162-R","url":null,"abstract":"Silicon (Si) supplementation permits plants to better deter infection. Supplementing hydroponically-propagated Nicotiana tabacum with 1 mM potassium silicate (K2SiO3) reduced necrotic lesion development on detached leaves by both Botrytis cinerea and Sclerotinia sclerotiorum. Previously, a family of Si-induced genes was identified in N. tabacum. These genes were members of the Solanaceous Histidine-Rich Defensin (HRD) superfamily and were termed NtHRD1s (the first identified family of Nicotiana tabacum Histidine-Rich Defensins). Defensins were originally identified to participate in innate immunity. Thus, the NtHRD1s were tested for antimicrobial effects on plant pathogens. Transient expression of NtHRD1 genes within Nicotiana benthamiana leaves restricted the development of necrotic lesions caused by B. cinerea and S. sclerotiorum. Thus, the NtHRD1s may be an additional Si-responsive factor conferring beneficial effects on plants.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Threat to Limber Pine (Pinus flexilis) Restoration in Alberta and Beyond: First Documentation of a Cronartium ribicola race (vcr4) Virulent to Cr4-Controlled Major Gene Resistance. 阿尔伯塔省及其他地区林缘松（Pinus flexilis）恢复面临的新威胁：Cronartium ribicola race (vcr4) 病毒对 Cr4 控制的主要抗性基因的首次记录。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-09-25 DOI: 10.1094/PHYTO-04-24-0129-R

Jun-Jun Liu, Richard A Sniezko, Sydney Houston, Genoa Alger, Jodie Krakowski, Anna W Schoettle, Robert Sissons, Arezoo Zamany, Holly Williams, Benjamin Rancourt, Angelia Kegley

{"title":"A New Threat to Limber Pine (Pinus flexilis) Restoration in Alberta and Beyond: First Documentation of a Cronartium ribicola race (vcr4) Virulent to Cr4-Controlled Major Gene Resistance.","authors":"Jun-Jun Liu, Richard A Sniezko, Sydney Houston, Genoa Alger, Jodie Krakowski, Anna W Schoettle, Robert Sissons, Arezoo Zamany, Holly Williams, Benjamin Rancourt, Angelia Kegley","doi":"10.1094/PHYTO-04-24-0129-R","DOIUrl":"https://doi.org/10.1094/PHYTO-04-24-0129-R","url":null,"abstract":"The coevolution of virulence reduces the effectiveness of host resistance to pathogens, posing a direct threat to forest species and their key ecosystem functions. This exacerbates the threat to limber pine (Pinus flexilis), an endangered species in Canada due to rapid declines mainly driven by white pine blister rust (WPBR) as caused by Cronartium ribicola. We present the first report on a new C. ribicola virulent race (designated vcr4) that overcomes limber pine major gene (Cr4) resistance (MGR). Field surveys found that three parental trees (pf-503, pf-508 and pf-2015-0070) were cankered with WPBR in Alberta, but their progenies showed MGR-related phenotypic segregation post-inoculation of avirulent race (Avcr4). Genotyping of their progenies using Cr4-linked DNA markers and genome-wide association study (GWAS) provided additional support that these cankered parental trees had Cr4-controlled MGR. To confirm the presence of vcr4, aeciospores were collected from the cankered pf-503 tree to inoculate resistant seedlings that had survived prior inoculation using Avcr4 race, as well as seedlings of two US seed parents, one previously confirmed with MGR (Cr4) and one non-MGR, respectively. All inoculated seedlings showed clear stem symptoms, confirming the virulent race is vcr4. These results provide insights into evolution of C. ribicola virulence, and reinforces caution on deployment of Cr4-controlled MGR. The information will be useful for designing a breeding program for durable resistance by layering both R genes with quantitative trait loci (QTLs) for resistance to WPBR in North America.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High genetic diversity and limited regional population differentiation in populations of Calonectria pseudoreteaudii from Eucalyptus plantations. 桉树种植园中 Calonectria pseudoreteaudii 种群的遗传多样性较高，区域种群分化有限。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-09-25 DOI: 10.1094/PHYTO-05-24-0154-R

WenWen Li, FeiFei Liu, ShuaiFei Chen, Michael J Wingfield, Tuan A Duong

{"title":"High genetic diversity and limited regional population differentiation in populations of Calonectria pseudoreteaudii from Eucalyptus plantations.","authors":"WenWen Li, FeiFei Liu, ShuaiFei Chen, Michael J Wingfield, Tuan A Duong","doi":"10.1094/PHYTO-05-24-0154-R","DOIUrl":"https://doi.org/10.1094/PHYTO-05-24-0154-R","url":null,"abstract":"Calonectria pseudoreteaudii causes a serious and widespread disease known as Calonectria leaf blight in Eucalyptus plantations of southern China. Little is known regarding the population biology or reproductive biology of this pathogen in the affected areas. The aims of this study were to investigate the genetic diversity, population structure and the reproductive mode of C. pseudoreteaudii from affected Eucalyptus plantations of southern China. Ten polymorphic SSR markers were developed for the species, and were used to genotype 311 isolates from eight populations. The mating types of all isolates were identified using the MAT gene primers. The results revealed a high level of genetic diversity of the pathogen in all investigated populations. Of the 90 multilocus genotypes detected, ten were shared between at least two populations. With the exception of one population from HuiZhou, GuangDong (7HZ), the most dominant genotype was shared in seven remaining populations. DAPC and population differentiation analyses showed that the 7HZ population was well differentiated from the others and that there was no significant differentiation between the remaining populations. AMOVA suggested that most molecular variation was within populations (86%). Index of association analysis was consistent with a predominantly asexual life cycle for C. pseudoreteaudii in the studied regions. Although both mating types were detected in seven of the eight populations, the MAT1-1/MAT1-2 ratios in these populations deviated significantly from the 1:1 ratio expected in a randomly mating population.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling Pinus massoniana's defense mechanisms against Bursaphelenchus xylophilus under aseptic conditions: A transcriptomic analysis. 揭示无菌条件下 Pinus massoniana 对 Bursaphelenchus xylophilus 的防御机制：转录组分析

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-09-16 DOI: 10.1094/PHYTO-06-24-0180-R

Jinghui Zhu, Kean-Jin Lim, Tianyu Fang, Chen Zhang, Jianren Ye, Li-Hua Zhu

{"title":"Unraveling Pinus massoniana's defense mechanisms against Bursaphelenchus xylophilus under aseptic conditions: A transcriptomic analysis.","authors":"Jinghui Zhu, Kean-Jin Lim, Tianyu Fang, Chen Zhang, Jianren Ye, Li-Hua Zhu","doi":"10.1094/PHYTO-06-24-0180-R","DOIUrl":"https://doi.org/10.1094/PHYTO-06-24-0180-R","url":null,"abstract":"Pine wilt disease (PWD) is caused by pine wood nematode (PWN, Bursaphelenchus xylophilus) and significantly impacts pine forest ecosystems globally. This study focuses on Pinus massoniana, an important timber and oleoresin resource in China, and is highly susceptible to PWN. However, the defense mechanism of pine trees in response to PWN remains unclear. Addressing the complexities of PWD, influenced by diverse factors like bacteria, fungi, and environment, we established a reciprocal system between PWN and P. massoniana seedlings under aseptic conditions. Utilizing combined second and third-generation sequencing technologies, we identified 3,718 differentially expressed genes post-PWN infection. Transcript analysis highlighted the activation of defense mechanisms via stilbenes, salicylic acid and jasmonic acid pathways, terpene synthesis, and induction of pathogenesis-related proteins and resistance genes, predominantly at 72 hours post-infection. Notably, terpene synthesis pathways, particularly the mevalonate pathway, were crucial in defense, suggesting their significance in P. massoniana's response to PWN. This comprehensive transcriptome profiling offers insights into P. massoniana's intricate defense strategies against PWN under aseptic conditions laid a foundation for future functional analyses of key resistance genes.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmodiophora brassicae effector PbPE23 induces necrotic responses in both host and non-host plants. 黄铜疫霉菌效应子 PbPE23 可诱导寄主植物和非寄主植物产生坏死反应。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-09-16 DOI: 10.1094/PHYTO-02-24-0064-R

Md Musharaf Hossain, Edel Pérez López, Christopher D Todd, Yangdou Wei, Peta C Bonham-Smith

{"title":"Plasmodiophora brassicae effector PbPE23 induces necrotic responses in both host and non-host plants.","authors":"Md Musharaf Hossain, Edel Pérez López, Christopher D Todd, Yangdou Wei, Peta C Bonham-Smith","doi":"10.1094/PHYTO-02-24-0064-R","DOIUrl":"https://doi.org/10.1094/PHYTO-02-24-0064-R","url":null,"abstract":"Plasmodiophora brassicae is an obligate biotroph that causes clubroot disease in cruciferous plants, including canola and Arabidopsis. In contrast to most known bacterial, oomycete and fungal pathogens that colonize at the host apoplastic space, the protist P. brassicae establishes an intracellular colonization within various types of root cells and secretes a plethora of effector proteins to distinct cellular compartments favourable for survival and growth of the pathogen during pathogenesis. Identification and functional characterization of P. brassicae effectors has been hampered by the limited understanding of this unique pathosystem. Here, we report a P. brassicae effector, PbPE23, containing a Ser/Thr kinase domain, that induces necrosis after heterologous expression by leaf infiltration in both host and non-host plants. While PbPE23 is an active kinase, the kinase activity itself is not required for triggering the necrosis in plants. PbPE23 shows a nucleocytoplasmic localization in Nicotiana benthamiana and its N-terminal 25TPdPAQKQ32 sequence, resembling the contiguous hydrophilic TPAP motif and Q-rich region in many Nep1-like proteins (NLPs) from plant-associated microbes, is required for the induction of necrosis. Further, transcript profiling of PbPE23 reveals its high expression at the transition stages from primary to secondary infection, suggesting its potential involvement in the development of clubroot disease.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0