谷胱甘肽通过调节拟南芥硫醇稳态促进对不明嗜糖变性的易感性。

IF 3.1 2区 农林科学 Q2 PLANT SCIENCES
M Shamim Hasan, Anika Damm, Muhammad U Ijaz, Divykriti Chopra, Anna Koprivova, Stanislav Kopriva, Florian M W Grundler, Shahid Siddique
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引用次数: 0

摘要

谷胱甘肽(l-γ-谷氨酰-l-半胱氨酸-甘氨酸)是在生物和非生物胁迫条件下调控许多植物过程的关键分子。然而,它在植物对土壤传播的病原体,特别是经济上重要的根结线虫(RKN; Meloidogyne spp.)的反应中的作用,在很大程度上仍未被探索。这些线虫是专性生物营养体,在寄主根部建立专门的摄食场所——多核巨细胞,操纵植物的生物过程。在这里,我们使用遗传(谷胱甘肽生物合成突变体)、生化(硫醇和camalexin测量)和药理学(外源性谷胱甘肽补充)方法的组合研究了谷胱甘肽在RKN感染期间拟南芥中的作用。我们发现,谷胱甘肽合成突变体(rax1, pad2, cad2和nrc2)的根中谷胱甘肽的消耗显著减少了瘿的形成和卵的产量,表明其在线虫感染中起重要作用。此外,外源施用谷胱甘肽增加了植物对RKN的敏感性。生化分析显示,降低的谷胱甘肽水平在感染过程早期破坏了半胱氨酸-谷胱甘肽平衡。然而,进一步的分析,包括camalexin测量和gstf6功能丧失线的感染试验,表明谷胱甘肽依赖的植物抗菌素camalexin对RKN寄生没有显著的贡献。这些发现强调了谷胱甘肽在RKN感染早期阶段维持硫醇稳态中的重要性,并表明控制谷胱甘肽水平可能是农业中控制线虫的潜在策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Glutathione Promotes Susceptibility to Meloidogyne incognita by Modulating Thiol Homeostasis in Arabidopsis.

Glutathione (l-γ-glutamyl-l-cysteinyl-glycine) is a key molecule that regulates numerous plant processes under both biotic and abiotic stress conditions. However, its role in plant responses to soil-borne pathogens, particularly the economically important root-knot nematodes (RKN; Meloidogyne spp.), remains largely unexplored. These nematodes are obligate biotrophs that establish specialized feeding sites-multinucleate giant cells-in host roots, manipulating plant biological processes. Here, we investigated the role of glutathione in Arabidopsis thaliana during RKN infection using a combination of genetic (glutathione biosynthetic mutants), biochemical (thiol and camalexin measurements), and pharmacological (exogenous glutathione supplementation) approaches. We found that glutathione depletion in roots of mutants in glutathione synthesis (rax1, pad2, cad2 and nrc2) significantly reduced gall formation and egg mass production, suggesting its important role in nematode infection. Additionally, the exogenous application of glutathione increased plant susceptibility to RKN. Biochemical analysis revealed that reduced glutathione levels disrupted the cysteine-glutathione balance early in the infection process. However, further assays, including camalexin measurements and infection assays with gstf6 loss-of-function lines, indicated that glutathione-dependent phytoalexins camalexin does not significantly contribute to RKN parasitism. These findings underscore the importance of glutathione in maintaining thiol homeostasis during the early stages of RKN infection and suggest that manipulating glutathione levels could be a potential strategy for nematode control in agriculture.

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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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