Preparative Biochemistry & Biotechnology最新文献

{"title":"Valorization of different agricultural wastes for yeast based Reactive Black 5 bioaccumulation.","authors":"Eda Açıkel, Aybüke Kut Yılmaz, Sevgi Ertuğrul Karatay","doi":"10.1080/10826068.2026.2710765","DOIUrl":"https://doi.org/10.1080/10826068.2026.2710765","url":null,"abstract":"<p><p>The textile industry generates large volumes of dye-laden effluents that pose risks to aquatic ecosystems and human health. This study evaluates the bioaccumulation of Reactive Black 5 (RB5) using <i>Candida boidinii</i> and <i>Kluyveromyces marxianus</i> yeast strains. Key operational parameters including initial pH, incubation time, dye concentration, and microbial growth were systematically investigated. In addition, the effects of low-cost agricultural wastes-carrot pomace (CP), industrial tea waste (ITW), and pumpkin pomace (PP)-as supplementary substrates were compared. Results showed that CP was the most effective substrate, with 100 g/L CP significantly enhancing RB5 removal. At pH 5.0 and 50 mg/L RB5, removal efficiencies reached 96.88% for <i>C. boidinii</i> and 81.76% for <i>K. marxianus</i> with CP, compared to 72.90% and 65.07% with PP. Although removal efficiency decreased at higher dye concentrations, the maximum dye uptake capacity (<i>q_m</i>) increased with initial RB5 concentration, reaching 52.52 mg/g for <i>C. boidinii</i> and 33.23 mg/g for <i>K. marxianus</i> over the tested range (≈50-600 mg/L). To our knowledge, this is the first report demonstrating RB5 removal by these yeasts using CP as a low-cost substrate. Overall, CP significantly improves yeast-based RB5 bioaccumulation and represents a promising, sustainable option for treating dye-contaminated wastewater.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.2,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148679896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal biodiversity and oxidative enzymes: nature-based solutions for lignin degradation and circular economy applications.","authors":"Antarikha Dutta, Neki Borang, Meera Yadav","doi":"10.1080/10826068.2026.2690415","DOIUrl":"https://doi.org/10.1080/10826068.2026.2690415","url":null,"abstract":"<p><p>Lignin is one of the most abundant yet recalcitrant biopolymers in nature, and its inefficient depolymerization remains a major bottleneck in lignocellulosic biomass valorization and sustainable biorefinery development. Although fungal oxidative enzymes have emerged as promising biocatalysts for lignin degradation, current knowledge remains fragmented across fungal diversity, catalytic mechanisms, enzyme engineering, and industrial translation. This review critically integrates recent advances in fungal ligninolytic systems, focusing on the structure, catalytic mechanisms, and synergistic interactions of major oxidative enzymes, including lignin peroxidase, manganese peroxidase, versatile peroxidase, laccase, and auxiliary oxidases such as aryl alcohol oxidase and glyoxal oxidase. Particular emphasis is placed on the comparative catalytic efficiency, operational limitations, and industrial feasibility of these enzymes in lignin depolymerization and biomass conversion. Recent developments in protein engineering, directed evolution, heterologous expression, immobilized enzyme systems, synthetic biology, and AI-assisted enzyme optimization are also discussed as emerging strategies to improve enzyme stability, substrate specificity, and process scalability. The review further highlights the role of fungal oxidative enzymes in bioremediation, lignocellulosic biorefineries, wastewater detoxification, biofuel production, and synthesis of value-added aromatic compounds. Importantly, it establishes a direct link between fungal ligninolytic systems and circular bioeconomy models through sustainable waste valorization and biomass-to-value conversion. Overall, this review provides an integrated perspective on the challenges, technological advancements, and future prospects of fungal oxidative enzymes for sustainable industrial biotechnology.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-26"},"PeriodicalIF":2.2,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148679912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the extraction and purification of plant polysaccharides: a review.","authors":"Rulan Qi, Beibei Ding, Mingyang Fang, Shensheng Xiao, Xuedong Wang","doi":"10.1080/10826068.2026.2710767","DOIUrl":"https://doi.org/10.1080/10826068.2026.2710767","url":null,"abstract":"<p><p>Plant polysaccharides have attracted considerable interest in natural product research due to their immunomodulatory, antitumor, and hypoglycemic activities. The development of efficient and gentle extraction and purification techniques is crucial for obtaining highly pure bioactive polysaccharides. This review systematically covers recent progress in extraction methods, including conventional hot water (simple but time‑consuming), acid/alkali (high yield but structure‑damaging), enzyme‑assisted, and physical field‑assisted techniques such as ultrasound and microwave, which provide higher efficiency under gentler conditions. For purification, a comparative analysis was conducted on the advantages and limitations of physical/chemical and biological methods for purifying polysaccharides. Furthermore, the review addresses current challenges, such as limited selectivity and throughput, and explores future directions, including the adoption of green solvents, intelligent process control, and integrated extraction-purification-evaluation platforms, with the objective of facilitating standardized production and high-value utilization.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148670466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and optimization of L-Asparaginase production from native halotolerant <i>Bacillus subtilis</i> bacteria isolated from Central Iran.","authors":"Kamran Hosseini, Parvin Akbarzadehlaleh, Somayeh Hallaj-Nezhadi, Mohsen Hassanpour, Mojtaba Khyrallahpour, Azita Dilmaghani","doi":"10.1080/10826068.2026.2673213","DOIUrl":"https://doi.org/10.1080/10826068.2026.2673213","url":null,"abstract":"<p><p>L-Asparaginase is an important enzyme with therapeutic applications. Current commercial enzymes from <i>E. coli</i> and <i>Erwinia chrysanthemi</i> have limitations, such as high glutaminase activity, creating a need for more stable alternatives. This study aimed to isolate and optimize the production of L-Asparaginase from native, halotolerant <i>Bacillus subtilis</i> strains (DAR and D6A) isolated from central Iran, to find an alternative source for this therapeutically important enzyme. The enzyme was initially isolated using polyethylene glycol (PEG) precipitation. Key factors affecting enzyme production were screened using the Plackett-Burman design and subsequently optimized via the Box-Behnken response surface methodology. PEG precipitation at concentrations of 15-20% for PEG 4000/6000 and 10-15% for PEG 8000/10000 was most effective for initial enzyme separation, yielding specific activities of 4.8 U/mg and 5.2 U/mg for strains DAR and D6A, respectively. Optimization revealed that the most influential factors for maximum L-Asparaginase production were 17.6 g/L NaCl, 4.99 g/L KH₂PO₄, and 10 g/L asparagine for strain DAR, and 1.49 g/L glucose, 10 g/L NaCl, and 9.98 g/L asparagine for strain D6A. The isolated halotolerant <i>B. subtilis</i> strains are capable of producing L-Asparaginase. PEG precipitation is a suitable method for initial purification, and statistical optimization successfully identified the critical culture conditions to enhance enzyme yield significantly. These strains represent promising sources for the production of this clinically valuable enzyme.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148670511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaerobic biodegradation of biopolymers for sustainable valorization of bioplastics: Integrated kinetic modeling and microbial community assessment.","authors":"Partha Kundu, Mariyam Muhammed, Ananthakrishnan G, Aryajyothis C, A V Anju","doi":"10.1080/10826068.2026.2708828","DOIUrl":"https://doi.org/10.1080/10826068.2026.2708828","url":null,"abstract":"<p><p>Anaerobic biodegradation of bioplastics has gained increasing attention as a sustainable strategy for waste valorization and renewable energy recovery within circular bio-economy systems. The present study investigated the anaerobic biodegradation of Poly lactic acid (PLA) pellet, PLA film, Polybutylene succinate (PBS), and commercially available bioplastics under controlled conditions at different temperatures and incubation periods. Biodegradation behavior was evaluated through cumulative gas production, visual observations, FTIR characterization, kinetic modeling, and microbial analysis. All biopolymer samples exhibited significant cumulative gas production within 20 days before reaching the stationary phase. Among the biopolymer materials, PLA film showed distinct degradation behavior with high total solids (92.5%) and volatile solids (75.4%), along with the highest cumulative gas production. FTIR analysis and observable structural changes further confirmed polymer degradation during anaerobic digestion. Microbial species associated with biodegradation were identified through PCR amplification, 16S rRNA sequencing, and phylogenetic analysis, revealing the involvement of <i>Bacillus sp</i> in the degradation process. The cumulative biogas production profiles of all biopolymers followed the Hill sigmoidal kinetic model with high correlation coefficients (<i>R²</i>: 0.9854, 0.9839, 0.9931, and 0.9772 for PLA pellet, PBS, PLA film, and bioplastic, respectively), indicating excellent model fitting accuracy. Moreover, a generalized anaerobic biodegradation model equation was developed for biopolymers. The findings demonstrated the potential applicability of anaerobic digestion for biopolymer waste valorization through simultaneous biodegradation and biogas generation, supporting sustainable waste management and circular bio-economy approaches.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148654100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-synthesis of palladium quantum dots anchored reduced graphene oxide as an efficient dye degrading and antibacterial nanoplatform.","authors":"K Mallikarjuna, I Neelakanta Reddy, L Veeranjaneya Reddy, Kiran Kumar Ganta, Abdullah A Alarfaj, Abdurahman H Hirad, C Parthasaradhi Reddy, Suresh V Chinni, Subash C B Gopinath, Cheolho Bai","doi":"10.1080/10826068.2026.2705566","DOIUrl":"10.1080/10826068.2026.2705566","url":null,"abstract":"<p><p>Phytogenic fabrication of nanostructures using bio-waste derivatives was potentially important due to its simple, ecofriendly and economical process. In addition to that, the construction and design of high surface two-dimensional nanostructures are significantly lime-lighted owing to their propound performance in catalytic, energy and environmental applications. The palladium quantum dots (Pd QDs) were anchored on reduced graphene oxide (rGO) nanostructures were prepared by using <i>Lychee</i> fruit peel extract under ultra-sonication process. The crystalline features and phase purity of this were analyzed through the X-ray diffraction (XRD) and peaks are labeled with (111), (200), (220), (311) and (222) facets were observed. The morphological features like size, shape and distribution of the Pd QDs (4 ± 1 nm) on rGO nanosheets were examined through transmission electron microscopic (TEM) studies. The oxidation states and chemical composition of prepared materials such as palladium and carbon and oxygen elements were studied with X-ray photoelectron spectroscopic (XPS) analysis. The prepared Pd QDs decorated rGO sheets performed the enhanced catalytic activities against pollutant degradation (4-nitrophenol (4-NP)). Moreover, the prepared nanostructures were significantly performed antibacterial properties against gram positive (<i>B. subtilis</i>) and gram negative (<i>E. coli</i>) bacterial strains. From the obtained results, the phytogenic method used for preparing large scale synthesis of metal QDs decorated graphene nanosheets for diverse applications like catalytic, energy, medicinal and environmental applications.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-7"},"PeriodicalIF":2.2,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response surface methodology based optimization of biomass and ultrasound assisted extraction and characterization of bioactive compounds from <i>Neochloris</i> sp. isolated from fresh water.","authors":"Amirthaashree Ananthapadmanaban, Arthi Sakthiyavel, Santhosh Sigamani, Dhandapani Ramamurthy, Sundaramoorthy Nagalingam","doi":"10.1080/10826068.2026.2705577","DOIUrl":"https://doi.org/10.1080/10826068.2026.2705577","url":null,"abstract":"<p><p>In this study, the microalgae <i>Neochloris</i> sp. is isolated from freshwater bodies. To increase the biomass of <i>Neochloris</i> sp. primary optimization studies were done for the selection of suitable carbon and nitrogen sources. Further optimizations were conducted by response surface methodology (Box Behnken method) for increased biomass production. <i>Neochloris</i> sp. grown in the presence of 0.04 g/L sodium acetate, 1.5 g/L urea, 7.5 pH, at 30 °C temperature, showed increased biomass. A second-order polynomial produced satisfactory biomass yield results (<i>P</i> < 0.0001). Results of the predicted and actual response were different, with better residual > 0.02%, and predicted <i>R</i>² 0.9839 and actual <i>R</i>² 0.9928 for biomass, which shows a more reliable model. The bioactive compounds are extracted through Ultra-Sound Assisted extraction from <i>Neochloris</i> sp. and observed through TLC to confirm the presence of carotenoids which separates at a retention factor value of 0.38. A phytochemical test for the extract showed positive results for terpenoids. HPLC was performed for characterization studies which separate at a retention time of 4.171 min. The extract shows maximum DPPH radical scavenging activity of 93%, ABTS of 92% and H₂O₂ scavenging activity of 64% at 120 µL/mL concentration. From this study, we can conclude that <i>Neochloris</i> sp.is a possible microalgae for producing antioxidants that can be used in the food industry as a natural coloring agent.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148621143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable sol-gel synthesis of zinc ferrite nanomaterial using natural surfactant with potent antimicrobial properties.","authors":"B Avila Josephine, G Kaladevi, A Ashwini, Subash C B Gopinath","doi":"10.1080/10826068.2026.2705573","DOIUrl":"https://doi.org/10.1080/10826068.2026.2705573","url":null,"abstract":"<p><p>Zinc ferrite nanoparticles have been synthesized via green gelation route employing a natural surfactant as a sustainable stabilizing additive. The usage of natural surfactants minimized the toxicity thereby offering an eco-friendly approach to synthesis of nanomaterials. The synthesized nanomaterials were systematically characterized by powder X-ray diffraction (PXRD), FT-IR, SEM-EDAX, UV-visible, and VSM techniques. PXRD peaks reveal the formation of cubic spinel structure with no impurities. FT-IR spectrum confirmed the characteristic functional groups present in spinel ferrite. The prepared nanomaterials showed agglomeration with wider flaky grains from SEM analysis. The EDAX confirmed the elemental compositions present in zinc ferrite nanomaterials. VSM studies showed low saturation magnetization, coercivity and remnant magnetization. The absorption maxima for zinc ferrite nanomaterial around 318 nm corresponds to the charge transfer from ligand to metal center. Besides, antibacterial and antifungal studies were evaluated using agar-disk diffusion method against pathogenic strains.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-6"},"PeriodicalIF":2.2,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148606581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ligninolytic enzyme activity of macromycetes: optimization of their production and degradation of malachite green and phenol red.","authors":"María Monserrat Chávez-Ramírez, Andrea Mendoza-Arceo, Wilberth Chan-Cupul","doi":"10.1080/10826068.2026.2706596","DOIUrl":"https://doi.org/10.1080/10826068.2026.2706596","url":null,"abstract":"<p><p>Ligninolytic macromycetes are important producers of oxidative enzymes with potential applications in the mycoremediation of synthetic dyes. However, the enzymatic potential of native tropical lignicolous fungi and the optimization of enzyme production for dye degradation remain poorly understood. This study aimed to isolate and characterize native lignicolous fungi, evaluate their ligninolytic enzyme production and dye-degrading [malachite green (MG) and phenol red (PR)] capacity, and optimize enzyme production using response surface methodology. Five fungal isolates were identified as <i>Trametes villosa</i>, <i>Trametes</i> sp., <i>Junghuhnia</i> sp., <i>Pycnoporus sanguineus</i>, and <i>Schizophyllum commune</i>. On PDA, <i>T. villosa</i> exhibited the highest mycelial growth rate (1.16 cm day^-1), whereas <i>P. sanguineus</i> showed the highest laccase activity (18.64 U L^-1). Under submerged fermentation, <i>P. sanguineus</i> produced the greatest H₂O₂ (418.9 mg L^-1), laccase (398.6 U L^-1), and lignin peroxidase (618.3 U L^-1) activities; while <i>Junghuhnia</i> sp. exhibited the highest manganese peroxidase (MnP; 32.9 U L^-1). Crude enzymatic extracts degraded MG and PR by 94.57% and 47.84%, respectively, within 72 hr. Box-Behnken optimization revealed that high glucose concentrations (30 g L^-1) enhanced laccase production in 0.86-fold increase, whereas low yeast extract (1.25 g L^-1) favored MnP synthesis in 5.10-fold increase. These findings identify native tropical fungi, particularly <i>P. sanguineus</i>, as promising candidates for enzyme-based wastewater treatment and environmental remediation.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148593127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, biochemical characterization, and industrial potential of a thermostable lipase from <i>Aeribacillus pallidus</i> IO3 isolated from a geothermal source.","authors":"Ikra Ozkan, Ahmet Adiguzel","doi":"10.1080/10826068.2026.2705568","DOIUrl":"https://doi.org/10.1080/10826068.2026.2705568","url":null,"abstract":"<p><p>Lipases obtained from thermophilic microorganisms are attracting great interest in the industrial field due to their stability under high-temperature conditions and their wide range of industrial applications. In this study, thermophilic bacterial strains were isolated from water and mud samples collected from Erzurum Ilica Hot Springs (Turkey). An isolate producing a potent extracellular lipase was selected, named IO3, and identified by 16S rRNA sequencing. Sequencing analysis revealed that this isolate showed 99% similarity to <i>Aeribacillus pallidus.</i> The lipase enzyme purified from the isolate was obtained using two comparative strategies. The conventional multi-stage chromatographic approach, which includes ammonium sulfate precipitation, ion exchange chromatography, and gel filtration chromatography, provided 9.3-fold purification with a 3.18% recovery. However, the alternative Three-Phase Partitioning (TPP) system provided rapid, single-step recovery with a significantly higher yield of 38.79%, although the purification fold remained 0.65 under the tested conditions. The molecular weight of the purified <i>A. pallidus</i> IO3 lipase was determined to be approximately 33.88 kDa by SDS-PAGE. The enzyme showed optimum activity at pH 8.0 and 50 °C and maintained considerable thermal stability; the enzyme retained significant activity levels (45-69%) even at high temperatures such as 70 °C and 80 °C after an incubation period of 120 minutes. Among the tested metal ions, Fe²⁺, Fe³⁺, Cu²⁺, and Zn²⁺ enhanced enzyme activity, while the enzyme showed stability in the presence of Li⁺ and Cu²⁺ ions. Lipase activity also increased in the presence of surfactants, while chloroform enhanced enzyme activity by 22-338% depending on solvent concentration. The enzyme showed the highest activity toward p-nitrophenyl palmitate. The enzyme was completely inhibited by DTNB, IAA, and EDTA. In addition, the enzyme retained low activity in the presence of <i>β</i>-mercaptoethanol. The Km and Vmax values were calculated as 0.46 mM and 51.44 µmol·min^-1·mg^-1, respectively, using nonlinear regression analysis. Overall, these findings indicate that <i>A. pallidus</i> IO3 lipase is a thermostable and chemically tolerant enzyme with potential applicability in industrial biocatalysis and detergent-related processes.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148585098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}