Preparative Biochemistry & Biotechnology最新文献_第5页

Comparative analysis of biodesulfurization of dibenzothiophene (DBT) and 4,6-dimethyl dibenzothiophene (4,6-DMDBT) by 4S pathway using molecular simulations. 利用分子模拟对二苯并噻吩（DBT）和 4,6-二甲基二苯并噻吩（4,6-DMDBT）通过 4S 途径进行生物脱硫的比较分析。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-03 DOI: 10.1080/10826068.2024.2448183

Pushpita Das, Umesh, Lepakshi Barbora, Vijayanand Suryakant Moholkar

{"title":"Comparative analysis of biodesulfurization of dibenzothiophene (DBT) and 4,6-dimethyl dibenzothiophene (4,6-DMDBT) by 4S pathway using molecular simulations.","authors":"Pushpita Das, Umesh, Lepakshi Barbora, Vijayanand Suryakant Moholkar","doi":"10.1080/10826068.2024.2448183","DOIUrl":"https://doi.org/10.1080/10826068.2024.2448183","url":null,"abstract":"In this paper, we have analyzed biodesulfurization of dibenzothiophene (DBT) and 4,6-dibenzothiophene (4,6-DMDBT) by 4S metabolic pathway using molecular simulations. Docking analysis revealed lower binding energies and inhibition constants (Ki) for 4,6-DMDBT and its metabolic intermediates with DSZ enzymes than DBT and its intermediates. The complexes of substrate and its metabolites with DSZ enzymes had higher stability for 4,6-DMDBT than DBT owing to lower RMSF values than apoprotein. The docking analysis revealed affinity of the inhibitors HBPS and HBP (for DBT) and DMHBPS and DMHBP (for 4,6-DMDBT) toward DSZ enzyme due to negative binding energies. Molecular dynamics simulations showed stability of several enzyme-inhibitor complexes. The inhibitory effect of DMHBPS on DSZC enzyme (Ki = 1.53 µM) and DMHBP on DSZB enzyme (Ki = 3.87 µM) was most marked. The inhibitory effect of HBP on DSZC and DSZB enzymes was moderate due to Ki of 6.36 and 7.93 µM, respectively. The inhibition effect of DMHBP on the DSZA enzyme was insignificant due to high Ki of 53.6 µM. In summary, higher stability of enzyme-substrate complexes and strong inhibition by DMHBPS and DMHBP (due to very low Ki) contribute to slower biodesulfurization of 4,6-DMDBT as compared to DBT.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-17"},"PeriodicalIF":2.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved doramectin production based on high-throughput screening and medium optimization in Streptomyces avermitilis. 基于高通量筛选和培养基优化改进阿维菌素链霉菌的多拉菌素生产。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-02 DOI: 10.1080/10826068.2024.2448181

Yu Wang, Huan He, Siqi Li, Limei Ren, Xiaobing Li, Xiaoru Wang

{"title":"Improved doramectin production based on high-throughput screening and medium optimization in Streptomyces avermitilis.","authors":"Yu Wang, Huan He, Siqi Li, Limei Ren, Xiaobing Li, Xiaoru Wang","doi":"10.1080/10826068.2024.2448181","DOIUrl":"10.1080/10826068.2024.2448181","url":null,"abstract":"Doramectin, a 16-membered macrocyclic lactone that is widely used in the treatment of mammalian parasitic diseases. Doramectin was produced by mutant Streptomyces avermitilis using cyclohexanecarboxylic acid as a precursor. As a semi-synthetic insecticidal agent produced, the production of doramectin was low, which could not be satisfy the demands of industrial fermentation. In this study, a high-yield mutant strain S. avermitilis DA-137 was screened from the starting strain S. avermitilis D-11 through a high-throughput screening strategy. S. avermitilis D-11 was treated with iterative atmospheric and room temperature plasma mutagenesis to induce mutations. Mutation strains were prescreened by spreading on enhanced doramectin-tolerance plates and were rescreened in 24-deep microtiter plates and via microplate readers to obtain high-producing strains. The resulting mutant strain S. avermitilis DA-137 produced 431.5 mg/L doramectin, a 187% increase compared to that of D-11, revealing mutagenesis and doramectin-tolerance screening is an efficient method to enhance doramectin production. Then, fermentation medium was optimized using the response surface method to improve doramectin production. In the optimized fermentation medium, the yield of doramectin was increased to 934.5 mg/L in shake flask. Furthermore, batch culture was carried out in a 50 L fermenter, and the yields of doramectin reached 1217 mg/L at 216 h, which was the highest yield reported to date. This study demonstrates a successful approach for enhancing doramectin production through high-throughput screening strategy and medium optimization, serving as a reference for increasing the yield of other macrocyclic lactone antibiotics.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel. 最大限度地提高多酚产量：芒果皮的超声波辅助提取和抗菌潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-10-02 DOI: 10.1080/10826068.2024.2411518

Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri

{"title":"Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel.","authors":"Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri","doi":"10.1080/10826068.2024.2411518","DOIUrl":"10.1080/10826068.2024.2411518","url":null,"abstract":"This study investigated the ultrasound-assisted extraction (UAE) techniques used to enhance the polyphenols and antioxidants of mango peel extract (MPE). Additionally, it explored the bacteriostatic activity of MPE against various microorganisms. The UAE method was optimized using response surface methodology (RSM) at different times, temperatures, and ratios, with optimal conditions found to be 35 minutes, 45 °C, and a 1:35 ratio. The optimized yield results for total polyphenol content (TPC) were 17.33 ± 1.57 mg GAE/g, total flavonoid content (TFC) was 12.14 ± 0.29 mg QE/g, and radical scavenging activity (RSA) was 72.11 ± 2.19%. These response models were extremely significant with p-values less than 0.05. MPE showed selective effectiveness against Bacillus cereus, Geobacillus stearothermophilus, and Escherichia coli (E. coli). The results highlight the potential of mango peel as a sustainable source of bioactive compounds, contributing to waste reduction in the food industry and the development of natural antimicrobial agents. This study contributes to further research on the application of MPE in processed foods.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"349-358"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate. 以麦麸为低成本底物，通过固态发酵法利用真菌 Pleurotus djamor 生产凝乳蛋白酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-09-02 DOI: 10.1080/10826068.2024.2399040

Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira

{"title":"Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate.","authors":"Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira","doi":"10.1080/10826068.2024.2399040","DOIUrl":"10.1080/10826068.2024.2399040","url":null,"abstract":"Proteases are enzymes that hydrolyze peptide bonds present in proteins and peptides. They are widely used for various industrial applications, such as in the detergent, food, and dairy industries. Cheese is one of the most important products of the dairy industry, and the coagulation stage is crucial during the cheese-making process. Enzymatic coagulation is the most common technique utilized for this purpose. Microbial enzymes are frequently used for coagulation due to their advantages in terms of availability, sustainability, quality control, product variety, and compliance with dietary and cultural/religious requirements. In the present study, we identified and subsequently characterized milk coagulant activity from the fungus Pleurotus djamor PLO13, obtained during a solid-state fermentation process, using the agro-industrial residue, wheat bran, as the fermentation medium. Maximum enzyme production and caseinolytic activity occurred 120 h after cultivation. When the enzyme activity against various protease-specific synthetic substrates and inhibitors was analyzed, the enzyme was found to be a serine protease, similar to elastase 2. This elastase-2-like serine protease was able to coagulate pasteurized whole and reconstituted skim milk highly efficiently in the presence and absence of calcium, even at room temperature. The coagulation process was influenced by factors such as temperature, time, and calcium concentration. We demonstrate here, for the first time, an elastase-2-like enzyme in a microorganism and its potential application in the food industry for cheese production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"278-284"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus. 嗜热革兰霉菌（Geobacillus kaustophilus）中一种恒温单酰基甘油脂肪酶的表达和特征。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-04 DOI: 10.1080/10826068.2024.2361147

Noriyuki Doukyu, Hayato Ito, Kugako Sugimoto

{"title":"Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus.","authors":"Noriyuki Doukyu, Hayato Ito, Kugako Sugimoto","doi":"10.1080/10826068.2024.2361147","DOIUrl":"10.1080/10826068.2024.2361147","url":null,"abstract":"Thermophilic Geobacillus kaustophilus HTA426 genome possesses a monoacylglycerol lipase (MAGL) gene. MAGLs can synthesize emulsifiers for use in the food and pharmaceutical industries from fatty acids and glycerol. They can also be used to analyze monoacylglycerol (MAG) levels in serum and food. The MAGL gene from strain HTA426 was artificially synthesized and heterologously expressed in Escherichia coli BL21(DE3). The recombinant His-tag fused MAGL (GkMAGL) was purified using a Ni2+-affinity column. The purified enzyme showed a temperature optimum at 65 °C and was stable up to 75 °C after 30 min incubation. In addition, the enzyme exhibited a pH optimum of 7.5 and was stable from pH 5.0 to 11.0. The enzyme hydrolyzed monoacylglycerols and showed the highest activity toward 1-monolauroylglycerol. The enzyme was stable in the presence of various organic solvents and detergents. The addition of Triton X-100 significantly increased GkMAGL activity. The thermal stability of the enzyme was higher than that of thermostable MAGL from Geobacillus sp. 12AMOR1 (12AMOR1_MAGL). Circular dichroism spectral analysis showed that the conformational stability of the GkMAGL was higher than that of 12AMOR1_MAGL at higher temperatures. These results indicate that the GkMAGL has useful features that can be used for various biotechnological applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"58-66"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New wheat straw fermentation feed: recombinant Schizosaccharomyces pombe efficient degradation of lignocellulose and increase feed protein. 新型小麦秸秆发酵饲料：重组小麦酵母菌（Schizosaccharomyces pombe）高效降解木质纤维素并增加饲料蛋白质。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-02 DOI: 10.1080/10826068.2024.2353637

Xihua Chen, Xiaoyu Liang, Na Shi, Lu He, Yi Ma, Daochen Zhu, Zhong Ni, Huayou Chen

{"title":"New wheat straw fermentation feed: recombinant Schizosaccharomyces pombe efficient degradation of lignocellulose and increase feed protein.","authors":"Xihua Chen, Xiaoyu Liang, Na Shi, Lu He, Yi Ma, Daochen Zhu, Zhong Ni, Huayou Chen","doi":"10.1080/10826068.2024.2353637","DOIUrl":"10.1080/10826068.2024.2353637","url":null,"abstract":"Wheat straw contains a high amount of lignin, hindering the action of cellulase and hemicellulase enzymes, leading to difficulties in nutrient absorption by animals from straw feed. However, currently, the biological treatment of straw relies primarily on fungal degradation and cannot be directly utilized for the preparation of livestock feed. This study focuses on enzymatic co-fermentation of wheat straw to produce high-protein, low-cellulose biological feed, integrating lignin degradation with feed manufacturing, thereby simplifying the feed production process. After the optimization using Box-Behnken Design for the feed formulation, with a glucose oxidase addition of 2.46%, laccase addition of 3.4%, and malonic acid addition of 0.6%, the wheat straw feed prepared in this experiment exhibited a true protein content of 9.35%. This represented a fourfold increase compared to the non-fermented state, and the lignocellulose degradation rate of wheat straw reached 45.42%. These results not only highlight the substantial enhancement in protein content but also underscore the significant advancement in lignocellulose breakdown. This formulation significantly enhanced the palatability and nutritional value of the straw feed, contributing to the industrial development of straw feed.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"36-44"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment. 利用实验统计设计优化新型肠球菌的外多糖生产。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-09-20 DOI: 10.1080/10826068.2024.2402337

Shivani Singh Gaur, Uday S Annapure

{"title":"Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment.","authors":"Shivani Singh Gaur, Uday S Annapure","doi":"10.1080/10826068.2024.2402337","DOIUrl":"10.1080/10826068.2024.2402337","url":null,"abstract":"The Exopolysaccharide (EPS) producing novel strains of Enterococcus previously isolated from the vaginal source of pregnant women were selected based on ropy structure formation. The two selected strains, E.villorum SB-2 and E.rivorum S22-3, were found to be producing 2.87 g/l and 3.14 g/l EPS, respectively, in the minimal media (M17 media) after 24-hour fermentation under anaerobic condition. Both the strains have probiotic properties and have the potential to be used for industrial applications. The production media and fermentation conditions were optimized to enhance the EPS production using the one-factor method, Placket-Burman factorial designing and Central composite design (CCD) of Response surface methodology (RSM). The most relevant factors affecting the EPS yield were sucrose, yeast extract and pH for E.villorum SB2 and sucrose, yeast extract and magnesium sulfate for the E.rivorum S22-3 as determined by Placket-Burman design, whose concentrations were further optimized using CCD. The optimized fermentation conditions gave the total EPS of 9.76 g/l (4 times the initial production) from E.villorum SB-2 and 7.74 g/l (2.5 times the initial production) from E.rivorum S22-3, respectively, after 36-hour incubation at 37 °C. These optimization studies might be helpful during scale-up process for the industrial scale production of these exopolysaccharide.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"297-308"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and stability verification of reference genes in Botrytis cinerea ZX2 fermentation. 肉毒芽孢杆菌 ZX2 发酵中参考基因的筛选和稳定性验证。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-10-11 DOI: 10.1080/10826068.2024.2405944

Yifan Wang, Dan Shu, Zhemin Li, Di Luo, Jie Yang, Tianfu Li, Xiaonan Hou, Qi Yang, Hong Tan

{"title":"Screening and stability verification of reference genes in Botrytis cinerea ZX2 fermentation.","authors":"Yifan Wang, Dan Shu, Zhemin Li, Di Luo, Jie Yang, Tianfu Li, Xiaonan Hou, Qi Yang, Hong Tan","doi":"10.1080/10826068.2024.2405944","DOIUrl":"10.1080/10826068.2024.2405944","url":null,"abstract":"Botrytis cinerea, an airborne plant pathogen, holds the potential to synthesize sesquiterpenes, which have been used for the industrial production of abscisic acid. Previously, through our genetic technology, we obtained strain ZX2, whose main product 1´,4´-trans-ABA-diol is physiologically active in plants. In this study, 50 L of fed fermentation was carried out with ZX2 strain to study the stability of expression of TUA, TUB, ATC, EF-1, GAPDH, UCE and GTP genes. Four kinds of software (GeNorm, NormFinder, BestKeeper and Delta Ct) were used to analyze the expression stability of candidate genes, and finally the best reference gene was screened by RefFinder. Based on the results, the ACT was the most stable gene. It was used to normalize the expression levels of two genes related to 1´,4´-trans-ABA-diol production (hmgr and bcaba3) when fed-batch fermentation. Guide the selection of appropriate internal reference genes during the fermentation process to accurately quantify the relative transcription levels of target genes in B.cinerea ZX2.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"341-348"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of enzymatic production of anti-skin aging biopeptides from white sorghum [Sorghum bicolor (L) Moench] grain. 从白高粱（Sorghum bicolor (L) Moench）谷物中优化酶法生产抗皮肤老化生物肽。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-07-01 DOI: 10.1080/10826068.2024.2366994

Tania P Castro-Jácome, Erik G Tovar-Pérez, Luz E Alcántara-Quintana

{"title":"Optimization of enzymatic production of anti-skin aging biopeptides from white sorghum [Sorghum bicolor (L) Moench] grain.","authors":"Tania P Castro-Jácome, Erik G Tovar-Pérez, Luz E Alcántara-Quintana","doi":"10.1080/10826068.2024.2366994","DOIUrl":"10.1080/10826068.2024.2366994","url":null,"abstract":"Recently, kafirins from white sorghum [Sorghum bicolor (L) Moench] grain have shown promise as a source of biopeptides with anti-skin aging effects (anti-inflammatory, antioxidant, and inhibition of photoaging-associated enzymes). This study employed response surface methodology (RSM) to optimize the extraction and enzymatic hydrolysis of kafirins (KAF) for the production of peptides with anti-skin aging properties. The optimization of conditions (reaction time and enzyme/substrate ratio) for liquefaction with α-amylase and hydrolysis of KAF with alcalase was performed using 32 complete factorial designs. Subsequently, ultrafiltered peptide extracts were obtained with molecular weights of 1-3 kDa (KAF-UF3) and lower than 1 kDa (KAF-UF1), which mainly contain hydrophobic amino acids (proline, leucine, isoleucine, phenylalanine, and valine) and peptide fractions with molecular weights of 0.69, 1.14, and 1.87 kDa. Consequently, the peptide extracts protected immortalized human keratinocytes (HaCaT cells) from ultraviolet B radiation (UVB)-induced damage by preventing the decrease and/or restoring the activity of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)]. Furthermore, KAF-UF3 and KAF-UF1 inhibited (20-29%) elastase and collagenase overactivity in UVB-exposed murine fibroblasts (3T3 cells). Thus, KAF-UF3 and KAF-UF1 exhibited behavior similar to that observed with glutathione (GSH), suggesting their potential as functional peptide ingredients in skincare products.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"81-92"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review. 从天然资源中提取类胡萝卜素的绿色和可持续技术：综述。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-10-20 DOI: 10.1080/10826068.2024.2402905

Surbhi Sharma, Anshika Dedha, Manju M Gupta, Nahar Singh, Arvind Gautam, Abha Kumari

{"title":"Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review.","authors":"Surbhi Sharma, Anshika Dedha, Manju M Gupta, Nahar Singh, Arvind Gautam, Abha Kumari","doi":"10.1080/10826068.2024.2402905","DOIUrl":"10.1080/10826068.2024.2402905","url":null,"abstract":"In recent years, driven by increasing consumer demand for natural and healthy convenient foods, the food industry has been shifting from synthetic to natural products. This shift is also reflected in the growing popularity of non-conventional extraction methods for pigments, which are favored for sustainability and environment-friendliness compared to conventional processes. This review aims to investigate the extraction of carotenoids from a variety of natural sources, including marine sources like fungus, microalgae, and crustaceans, as well as widely studied plants like tomatoes and carrots. Additionally, it delves into the recovery of valuable carotenoids from waste products like pomace and peels, highlighting the nutritional and environmental benefits. The review also emphasizes the role of green solvents such limonene, vegetable oils, ionic liquids, supercritical fluids, and natural deep eutectic solvents in effective and ecologically friendly carotenoid extraction. These technologies support the ideas of a circular and sustainable economy in addition to having a smaller negative impact on the environment. Overall, the present study highlights the crucial importance of green extraction technologies in achieving the dual goals of sustainability and public safety.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"245-277"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0