Preparative Biochemistry & Biotechnology最新文献_第5页

Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems. 在微生物宿主系统中可溶性表达重组抗体片段的最新进展。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-28 DOI: 10.1080/10826068.2024.2394446

Preeti Saroha, Rucha S Patil, Anurag S Rathore

{"title":"Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems.","authors":"Preeti Saroha, Rucha S Patil, Anurag S Rathore","doi":"10.1080/10826068.2024.2394446","DOIUrl":"10.1080/10826068.2024.2394446","url":null,"abstract":"Recombinant fabs dominate the pharmaceutical pipelines today with microbial host systems continuing to be a major contributor toward their production. Escherichia coli is a versatile host for recombinant protein expression due to its simplicity, affordability, and ability to be cultivated at high cell density. It is particularly suitable for non-glycosylated proteins and small proteins. Despite the aforementioned benefits, the use of E. coli as the host for the synthesis of recombinant antibody fragments often suffers from low yield and reduced activity. In most cases, proteins are expressed as inclusion bodies and need to undergo refolding to achieve their active forms and this refolding step is generally low-yielding. In this article, we review the various approaches that researchers have taken to enhance the production of recombinant antibody fragments in E. coli. Molecular biology-oriented approaches such as cloning, chaperone-mediated folding, and host cell screening as well as process optimization involving examination of process parameters, media, and feeding have been addressed.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"131-140"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142086245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 DOI: 10.1080/10826068.2025.2457545

Pankaj D Parab, Rakhee D Khandeparker

{"title":"Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential.","authors":"Pankaj D Parab, Rakhee D Khandeparker","doi":"10.1080/10826068.2025.2457545","DOIUrl":"https://doi.org/10.1080/10826068.2025.2457545","url":null,"abstract":"Microbial xylanases are xylan hydrolyzing enzymes which has congregated attention due to their immense potential in many industries. The current study is focused on xylanase from bacterial isolate identified as Bacillus sp. NIORKP76 on an inexpensive agro-industrial waste (wheat bran) under solid-state fermentation. The isolate showed maximum xylanase production in growth medium supplemented with phosphate, NaCl and NH4Cl at 64 mM, 15 mg/mL and 0.3 mg/mL respectively which was maintained at 1:3 substrate to moisture ratio (w/v). Using optimized conditions, the maximum xylanase production in wheat bran was achieved in 72 h at room temperature 28 ± 2 °C. Xylanase exhibited pH optimum of 8.0 and retained 92% of its residual activity after 24 h incubation at pH 8. Xylanase displayed optimum activity at 60 °C, whereas it remained unhindered even after 12 h of incubation period at 30 and 40 °C. The xylanase isolated in this study was purified to homogeneity with a molecular weight of approximately 28 kDa. Xylanase from Bacillus sp. NIORKP76 was potentially converting agro-residues into biofuel feedstock. Pretreated wheat bran produced the highest yield fermentable sugars (141 mg/g), after 8 h incubation with 5 U/g xylanase and at 40 °C.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the production of L-proline in recombinant Escherichia coli BL21 by metabolic engineering. 通过代谢工程提高重组大肠杆菌 BL21 中 L-脯氨酸的产量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-10 DOI: 10.1080/10826068.2024.2378104

Jiajie Lu, Bing Fu, Zhiwen Zhu, Chuyang Yan, Fuyao Guan, Peize Wang, Ping Yu

引用次数: 0

Highly efficient strategy of lipopolysaccharide (LPS) decontamination from rHBsAg: synergistic effect of enhanced magnetic nanoparticles (MNPs) as an LPS affinity adsorbent (LAA) and surfactant as a dissociation factor. 从 rHBsAg 中清除脂多糖（LPS）的高效策略：增强型磁性纳米颗粒（MNPs）作为 LPS 亲和吸附剂（LAA）和表面活性剂作为解离因子的协同效应。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-13 DOI: 10.1080/10826068.2024.2377326

Alireza Kavianpour, Seyed Nezamedin Hosseini, Mohsen Ashjari, Maryam Khatami, Taravatsadat Hosseini, Hosnsa Soleimani

{"title":"Highly efficient strategy of lipopolysaccharide (LPS) decontamination from rHBsAg: synergistic effect of enhanced magnetic nanoparticles (MNPs) as an LPS affinity adsorbent (LAA) and surfactant as a dissociation factor.","authors":"Alireza Kavianpour, Seyed Nezamedin Hosseini, Mohsen Ashjari, Maryam Khatami, Taravatsadat Hosseini, Hosnsa Soleimani","doi":"10.1080/10826068.2024.2377326","DOIUrl":"10.1080/10826068.2024.2377326","url":null,"abstract":"The interaction of lipopolysaccharide with a recombinant protein is a serious bottleneck, particularly in the purification step of bioprocessing. Recombinant hepatitis B surface antigen (rHBsAg), the active ingredient of the hepatitis B vaccine, is probably contaminated by extrinsic LPS like other biopharmaceuticals. This research intends to eliminate LPS from its mixture with rHBsAg efficiently. Immobilized polymyxin B on magnetic nanoparticles (PMB-MNPs) was synthesized and implemented as an enhanced LPS affinity adsorbent (LAA). The 20-80 EU/dose binary samples with and without surfactant were applied to PMB-MNPs. Formerly, dynamic light scattering (DLS) and transmission electron microscopy (TEM) were examined on the samples to qualitatively show the dissociation effect of the surfactant. Considering the high potential interaction of LPS with HBsAg, the dissociation effects of 0.5 and 1.5% Tween 20 on the binary samples were assessed using immunoaffinity chromatography (IAC) as a quantification tool. The dissociation effect of Tween 20 substantially diminished the interaction, leading to a proportional increase of free LPS up to 66%. The synergetic effect of Tween 20 and privileged LAA was highly effective in eliminating more than 80% of LPS with a remarkable LPS clearance factor of 5.8 and a substantial protein recovery rate of 97%.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"150-159"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial neural networks (ANN)-genetic algorithm (GA) optimization on thermosonicated achocha juice: kinetic and thermodynamic perspectives of retained phytocompounds. 人工神经网络（ANN）-遗传算法（GA）对热渗阿楚茶汁的优化：保留植物化合物的动力学和热力学视角。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-12 DOI: 10.1080/10826068.2024.2378101

Puja Das, Prakash Kumar Nayak, Radha Krishnan Kesavan

{"title":"Artificial neural networks (ANN)-genetic algorithm (GA) optimization on thermosonicated achocha juice: kinetic and thermodynamic perspectives of retained phytocompounds.","authors":"Puja Das, Prakash Kumar Nayak, Radha Krishnan Kesavan","doi":"10.1080/10826068.2024.2378101","DOIUrl":"10.1080/10826068.2024.2378101","url":null,"abstract":"The extraction of phytocompounds from Achocha (Cyclanthera pedata) vegetable juice using traditional methods often results in suboptimal yields and efficiency. This study aimed to enhance the extraction process through the application of thermosonication (TS). To achieve this, an artificial neural network (ANN) and a genetic algorithm (GA) were utilized to simulate and optimize the process parameters. The study investigated the influence of ultrasonic amplitude (30%-50%), temperature (30 °C-50 °C), and sonication duration (15-60 min) on total polyphenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AOA), and ascorbic acid content (AA). Remarkably, the ANN-GA optimization resulted in optimal TS conditions: an ultrasonic amplitude of 40%, a temperature of 40 °C, and a sonication duration of 30 min. Subsequent analysis of extraction kinetics and thermodynamics across various temperatures (30 °C-50 °C) and extraction times (0-30 min) demonstrated R2 (0.98821) and χ2 (1.74773) for TPC with activation energy (Ea) 26.0456, R2 (0.99906) and χ2 (0.07215) for TFC with Ea 26.2336, R2 (0.99867) and χ2 (0.03003) for AOA with Ea 26.0987, R2 (0.99731) and χ2 (0.13719) for AA with Ea 26.0984, validating the pseudo second-order kinetic model. These findings indicate that increased temperature enhances the saturation concentration and rate constant of phytochemical extraction.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"171-186"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of lyoprotectant for recombinant human acidic fibroblast growth factor by response surface methodology. 用响应面方法优化重组人酸性成纤维细胞生长因子的冻干保护剂。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-19 DOI: 10.1080/10826068.2024.2378098

Qiwen Huang, Jing Hu, Dongjie Mei, Guopan Li, Jun Rong

{"title":"Optimization of lyoprotectant for recombinant human acidic fibroblast growth factor by response surface methodology.","authors":"Qiwen Huang, Jing Hu, Dongjie Mei, Guopan Li, Jun Rong","doi":"10.1080/10826068.2024.2378098","DOIUrl":"10.1080/10826068.2024.2378098","url":null,"abstract":"Recombinant human acidic fibroblast growth factor (rh-aFGF) is a widely used biological product, but it is unstable and its biological activity is easy to decrease. In order to maintain the long-term stability and biological activity of rh-aFGF, based on the response surface method, the freeze-drying characterization and cell proliferation rate of rh-aFGF freeze-dried powder were evaluated by scoring and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay in this study. The optimal concentrations of trehalose, glycine and BSA were optimized, and the optimal formulation was verified by regression experiment. The results showed that trehalose, glycine and BSA had significant effects on the characterization of lyophilized rh-aFGF and cell proliferation. The optimal formulation of 5.7% trehalose, 2.04% glycine and 1.98%BSA combined with rh-aFGF could achieve the optimal freeze-dried characterization and biological activity. Using the best formulation to verify, the freeze-dried formability index of the freeze-dried powder was 23.35, and the rate of cell proliferation was 43.59%, which was close to the expected 23 and 41.69%. This study determined a freeze-dried formulation of rh-aFGF that meets the requirements of freeze-dried formalization integrity and maintains biological activity, providing reliable support for the subsequent development of related drugs.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"160-170"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141724341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 DOI: 10.1080/10826068.2024.2441914

Yao Wen, Xiangyi Yan, Haiyun Chen

{"title":"Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides.","authors":"Yao Wen, Xiangyi Yan, Haiyun Chen","doi":"10.1080/10826068.2024.2441914","DOIUrl":"https://doi.org/10.1080/10826068.2024.2441914","url":null,"abstract":"For the valorization of Eucommia ulmoides (EU) for the functional food industry, the process of ultrasonic-assisted deep eutectic solvent (DES) extraction of EU polysaccharides (EUP) was optimized by response surface methodology. After response surface analysis and experimental verification, the optimum extraction conditions were as follows: the molar ratio of choline chloride to oxalic acid was 0.9:1, the water content of DES was 40.28%, the ratio of material to liquid was 1:22 g/mL, the time was 90 min, and the power was 320 W. The extraction rate of EUP was 1.71%, which was higher than the extraction rate of 0.75% by ultrasonic water extraction under the same conditions, and the optimization effect was better. After DEAE column gradient elution and Sephadex G-75 gel column chromatography, the elution curve and a refined polysaccharide (EUP-1) were obtained. The IC50 values of EUP-1 against α-glucosidase and α-amylase were 0.091 and 0.011 mg/mL. The IC50 values of EUP-1 on DPPH and ABTS·+ scavenging were 0.065 and 0.065 mg/mL. The monosaccharide composition of EUP-1 was analyzed as mannose, rhamnose, glucuronic acid, and galacturonic acid in the following molar ratio: 1.00: 1.28: 1.53: 1.88.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The improved purification technique for isolation of the novel CGTase from the alkaliphilc strain Caldalkalibacillus mannanilyticus IB-OR17-B1. 从嗜碱性菌株 Caldalkalibacillus mannanilyticus IB-OR17-B1 中分离新型 CGT 酶的改良纯化技术。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-06 DOI: 10.1080/10826068.2024.2386558

P Yu Milman, E A Gilvanova, G E Aktuganov

{"title":"The improved purification technique for isolation of the novel CGTase from the alkaliphilc strain Caldalkalibacillus mannanilyticus IB-OR17-B1.","authors":"P Yu Milman, E A Gilvanova, G E Aktuganov","doi":"10.1080/10826068.2024.2386558","DOIUrl":"10.1080/10826068.2024.2386558","url":null,"abstract":"Cyclodextrin-glucanotransferase (CGTase, EC 2.4.1.19) is a multifunctional enzyme that catalyzes many enzymatic reactions including cyclization, binding, disproportionation and hydrolysis reactions, playing an important role in the enzymatic synthesis of compounds that are widely used in agriculture, pharmaceuticals, food, chemical and biotechnology industries. The present research is aimed to optimize the purification protocol for the extracellular CGTase of alkaliphilc bacterial strain Caldalkalibacillus mannanilyticus IB-OR17-B1 guaranteeing the enzyme homogeneity and its high yield. The improved combination of ultrafiltration and corn-starch (5% w/v) affinity sorption techniques resulted to mild and rapid isolation of electrophoritically homogenic enzyme at 18 × increase of its specific activity and yield 56%. The developed two-step procedure instead the practiced tree-step one using commonly ion-exchange chromatography as final purification technique highly contributes in advance of cost-effectiveness for industrial production and isolation of valuable CGTases.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"223-229"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, purification, and physio-chemical characterization of melanin pigment from nigerseed hulls (Guizotia abyssinica). 从黑木耳（Guizotia abysinica）中分离、提纯和鉴定黑色素。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-12 DOI: 10.1080/10826068.2024.2376579

Sunil Kumar B T, Umesh H Hebbar, Sridevi Annapurna Singh

{"title":"Isolation, purification, and physio-chemical characterization of melanin pigment from nigerseed hulls (Guizotia abyssinica).","authors":"Sunil Kumar B T, Umesh H Hebbar, Sridevi Annapurna Singh","doi":"10.1080/10826068.2024.2376579","DOIUrl":"10.1080/10826068.2024.2376579","url":null,"abstract":"Plant melanin, a natural pigment, has gained significant attention recently due to its potential therapeutic and industrial applications. In this study, melanin pigments were extracted from Nigerseed hulls (NH) via alkali and acid extraction methods, followed by acid hydrolysis, organic solvent treatment, and repeated precipitation. The solubility of NH melanin was assessed, revealing solubility in alkali and dimethyl sulfoxide (DMSO) but insolubility in other common organic solvents. High-performance liquid chromatography (HPLC) was employed to measure the purity of NH melanin in comparison to standard melanin, while elemental analysis indicated a similarity between melanin extracted from nigerseed hulls and the standard counterpart. LC-MS data revealed a molecular weight of NH melanin. Furthermore, the stability of melanin was evaluated under varying conditions including temperature, oxidants, reducing agents, light exposure, and metal ion presence. Results demonstrated significant effects of Mg2+, Cu2+, and Fe2+ metal ions on melanin stability, with a minor effect observed for Ca2+, while sodium hyposulfite was found to destabilize the pigments. Our findings suggest that nigerseed hulls hold promise as a novel source for efficient melanin production, with potential applications in the food sector, food packaging, and biomedical fields.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"141-149"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced temperature control in ethanol fermentation using a PSO-PID controller with split-range control strategy. 采用分程控制策略的 PSO-PID 控制器在乙醇发酵过程中实现先进的温度控制。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-03 DOI: 10.1080/10826068.2024.2381761

Raju Yerolla, Suhailam P, Chandra Shekar Besta

{"title":"Advanced temperature control in ethanol fermentation using a PSO-PID controller with split-range control strategy.","authors":"Raju Yerolla, Suhailam P, Chandra Shekar Besta","doi":"10.1080/10826068.2024.2381761","DOIUrl":"10.1080/10826068.2024.2381761","url":null,"abstract":"Global energy demand is experiencing a notable surge due to growing energy security. Renewable energy sources, like ethanol, are becoming more viable. In the present study, the application of a PSO-PID (Particle Swarm Optimization - Proportional Integral Derivative) controller with a split-range control strategy was suggested for the regulation of temperature within the fermentation system. To optimize performance, a POS-PID controller with a split-range arrangement utilizing two control valves for hot and cold utilities was constructed. The study began by examining the open-loop dynamic response of the system to inlet temperature and concentration disturbances during ethanol production fermentation. Subsequently, a transfer function model was developed through linearization at the steady-state operating point. The split-range controller structure, implemented by optimizing the PSO-PID controller parameters using PSO, effectively demonstrated temperature control in simulations of a nonlinear model. In this investigation, the ethanol fermentation system was modeled as a CSTR using a modified Monod equation for microbial growth kinetics. Various dynamic behavioral disturbances were explored and verified in the model with plant data in this study. The simulation model results were validated through plant data. The proposed method showed superior closed-loop performance with respect to errors, with the actuators proving to be effective than other reported methods for temperature control.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"196-209"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0