Preparative Biochemistry & Biotechnology最新文献_第6页

Ultrasound-assisted extraction and purification of B. harveyi for the development of bioactive wound healing gel: In vitro evaluation. 超声辅助提取纯化哈氏菌用于生物活性伤口愈合凝胶的研制：体外评价。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-24 DOI: 10.1080/10826068.2026.2706599

Bavani Kasi Murugan, Renganathan Sahadevan, Hemavathy R V, Sangeetha Subramanian

{"title":"Ultrasound-assisted extraction and purification of B. harveyi for the development of bioactive wound healing gel: In vitro evaluation.","authors":"Bavani Kasi Murugan, Renganathan Sahadevan, Hemavathy R V, Sangeetha Subramanian","doi":"10.1080/10826068.2026.2706599","DOIUrl":"https://doi.org/10.1080/10826068.2026.2706599","url":null,"abstract":"Marine macroalgal biomass is abundant and contains multifunctional bioactive components. Bostrychia harveyi, one of the underutilized seaweeds, was collected and extracted by the ultrasonication process. UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and high-performance liquid chromatography (HPLC) were carried out to confirm functional groups and structural features. A novel wound healing gel was prepared using carrageenan incorporated with a flavonoid-rich extract from B. harveyi. The bioactivity of the extract and gel was assessed with standard in vitro assays. 2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) of the gel and ascorbic acid (standard) showed inhibitory concentrations (IC50) of 67.05 µg/mL and 50.48 µg/mL. The phosphomolybdenum reduction activity of gel and ascorbic acid showed inhibitory concentrations (IC50) of 61.71 and 48.51 µg/mL. The bovine serum albumin denaturation by the gel and diclofenac sodium exhibited a maximum inhibition of 83.47% and 93.6% at 100 µg/mL, confirming a significant anti-inflammatory property. The antibacterial activity was confirmed using an agar well diffusion assay, indicating maximum inhibition of E. coli compared to S. aureus. The scratch wound assay using the L929 fibroblast showed 98.25% scratch closure after 48 hours, highlighting the preliminary effectiveness of the gel through its natural bioactive compounds and serving as a sustainable alternative in wound healing.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-27"},"PeriodicalIF":2.2,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148585122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and in vitro biological activities of exopolysaccharides from sugarcane waste-derived Weissella paramesenteroides. 甘蔗废弃物副小肠魏氏菌外多糖的鉴定及体外生物活性研究。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-21 DOI: 10.1080/10826068.2026.2693876

Poorvaja Subramanian, Preethi Kathirvel

{"title":"Characterization and in vitro biological activities of exopolysaccharides from sugarcane waste-derived Weissella paramesenteroides.","authors":"Poorvaja Subramanian, Preethi Kathirvel","doi":"10.1080/10826068.2026.2693876","DOIUrl":"https://doi.org/10.1080/10826068.2026.2693876","url":null,"abstract":"Exopolysaccharides (EPS) are microbial biopolymers composed primarily of complex carbohydrates and are valued for their biocompatibility, biodegradability, and diverse biological activities. In this study, lactic acid bacteria were isolated from sugarcane waste, and a potent EPS-producing strain was identified as Weissella paramesenteroides through 16S rRNA gene sequencing. The sequence was deposited in GenBank under accession number PV786832. EPS production was optimized using a One-Factor-At-a-Time (OFAT) approach. Maximum EPS yield was obtained at pH 7, 30 °C, and 48 h incubation, with sucrose and yeast extract serving as the most effective carbon and nitrogen sources, respectively. Chemical analysis revealed a total carbohydrate content of 90% and a residual protein content of 3.7%. Structural characterization confirmed the presence of characteristic polysaccharide functional groups in the EPS. The EPS exhibited antibacterial activity against both Gram-positive and Gram-negative pathogens and demonstrated antioxidant properties, with a DPPH IC50 value of 188.6 µg/mL, ferric reducing antioxidant activity of 51.47 µg Trolox equivalents, and total antioxidant capacity of 160.02 ± 2.91 µg AAE/mL extract. Furthermore, the EPS showed dose-dependent anti-inflammatory activity with an IC50 value of 403.9 µg/mL. These findings highlight the bioactive potential of EPS from W. paramesenteroides and support its further exploration for biotechnological applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-19"},"PeriodicalIF":2.2,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the proteinase K digest improves yield and integrity of DNA extracted from formalin-fixed, paraffin-embedded tissue by an automated robot. 优化蛋白酶K消化提高了自动化机器人从福尔马林固定石蜡包埋组织中提取DNA的产量和完整性。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-21 DOI: 10.1080/10826068.2026.2697944

Camille Bellora, William Mathieson

{"title":"Optimizing the proteinase K digest improves yield and integrity of DNA extracted from formalin-fixed, paraffin-embedded tissue by an automated robot.","authors":"Camille Bellora, William Mathieson","doi":"10.1080/10826068.2026.2697944","DOIUrl":"https://doi.org/10.1080/10826068.2026.2697944","url":null,"abstract":"ABSTRACTAutomation is a requisite for laboratories performing large numbers of DNA extractions, but it can compromise DNA yield and/or integrity compared to manual extractions. We addressed this problem by optimizing the manual Proteinase K digest of our Chemagic MSM I robot, which performs automated DNA extractions from FFPE tissue. We sequentially optimized the volume of Proteinase K, digest time, temperature, thermomixing during digestion and the number of sections per extraction. We also evaluated using carrier RNA to enhance yield. DNA was quantified, purity-assessed and integrity-assayed at each step. The optimized protocol was validated using 24 FFPE blocks. Sequential optimization returned a 24-fold increase in DNA yield, but purity was slightly poorer. DNA integrity, assessed by DNA Integrity Numbers, qPCR and multiplex PCR, was also improved. A break point can be added between the Proteinase K digest and the DNA extraction (with samples stored at -80 °C). Carrier RNA increased DNA yield. The performance of a high-throughput robot that extracts DNA from FFPE with suboptimal efficiency can be radically improved, particularly by optimizing the Proteinase K digest step. Users should not assume the manufacturer's protocol is optimal.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148549999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in modification strategies for polymer-based biocarriers in biofilm wastewater treatment. 聚合物基生物载体在生物膜废水处理中的改性策略研究进展。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-19 DOI: 10.1080/10826068.2026.2689403

Dexin Su, Yupeng Chen

引用次数: 0

Gene expression, enzyme purification and physicochemical characterization of Weissella confusa P2 glucansucrase. 魏氏菌P2葡聚糖酶的基因表达、酶纯化及理化性质研究。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-19 DOI: 10.1080/10826068.2026.2706603

Jinlong Wang, Jing Jiang, Gubo Gao, Renpeng Du, Dan Zhao

{"title":"Gene expression, enzyme purification and physicochemical characterization of Weissella confusa P2 glucansucrase.","authors":"Jinlong Wang, Jing Jiang, Gubo Gao, Renpeng Du, Dan Zhao","doi":"10.1080/10826068.2026.2706603","DOIUrl":"https://doi.org/10.1080/10826068.2026.2706603","url":null,"abstract":"In this study, the glucansucrase-encoding gene (dsr) from Weissella confusa P2 was cloned and heterologously expressed in Escherichia coli BL21(DE3). A 4542 bp fragment encoding 1510 amino acids was obtained, and the recombinant enzyme exhibited a molecular weight of approximately 161 kDa with a theoretical pI of 4.74. After Ni-IDA affinity chromatography, the specific activity reached 300.19 U/mg with a purification fold of 18.43. Enzymatic characterization revealed that the recombinant glucansucrase displayed optimal activity at pH 5.5 and 30 °C, and retained over 70% relative activity after 48 h incubation at 10-30 °C. The enzyme was significantly activated by Ca2+, while strongly inhibited by Ni2+, Cd2+, SDS, EDTA, DTT, and high concentrations of organic solvents. Kinetic analysis using sucrose as substrate yielded Km of 0.56 mM and Vmax of 4.57 U/mg, indicating exceptionally high substrate affinity. Using this recombinant enzyme, exopolysaccharide (EPS) was synthesized in vitro, achieving a yield of 59.23 ± 0.21 g/L, representing an 18.79% improvement over EPS synthesis by the wild-type strain. These findings enrich the genetic resources of glucansucrase, provide a promising biocatalyst for efficient EPS production, and establish a foundation for the application of glucansucrase and its polysaccharides products in the food and biotechnology industries.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.2,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective production of vi polysaccharide through high-cell-density fermentation using animal component-free media for typhoid vaccines. 利用不含动物成分的培养基，通过高密度发酵高效生产伤寒疫苗所需的vi多糖。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-17 DOI: 10.1080/10826068.2026.2703126

Anirban Ray, Charles David, Pradeep Gupta, Neeraj Joshi, Tarun Sharma

{"title":"Cost-effective production of vi polysaccharide through high-cell-density fermentation using animal component-free media for typhoid vaccines.","authors":"Anirban Ray, Charles David, Pradeep Gupta, Neeraj Joshi, Tarun Sharma","doi":"10.1080/10826068.2026.2703126","DOIUrl":"https://doi.org/10.1080/10826068.2026.2703126","url":null,"abstract":"Salmonella enterica serovar Typhi remains a major public health concern that requires an effective strategy to produce vaccines. This study reports the development of a high cell density fermentation (HCD) process to increase the production of Vi- polysaccharide (ViPS). The strain has been confirmed by means of phenotypic, biochemical (VITEK 2), and serological methods. Media optimization identified soybean peptone as a cost-effective and non-animal component alternative that promotes robust growth. The optimized fed-batch fermentation process increased the cell density from OD600 19-20 under low-cell-density conditions to OD600 39-42.1 under high-cell-density conditions, resulting in an approximately 1.7-fold increase in Vi polysaccharide (ViPS) yield i.e., from 165-187.5 mg/L to 283.75-302.5 mg/L. A scaling to 30 L fermentation tanks yielded comparable yields (290-320 mg/L), demonstrating the robustness of the process. Thermal inactivation at 65 °C for 30 min achieved complete cell inactivation without compromising the integrity of the polysaccharide, as predicted by the predictive model (R2 = 0.9947). NMR analysis confirms the structural integrity. The purified ViPS met WHO specifications, indicating suitability for cost-effective and scalable typhoid vaccine production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.2,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148472469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated statistical and machine-learning optimization for enhanced heparosan production by Lactococcus lactis. 乳酸乳球菌提高肝素聚糖产量的综合统计和机器学习优化。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-17 DOI: 10.1080/10826068.2026.2703129

Siddharth Guhan, Thilakraj S, Senthilkumar Sivaprakasam, Rengesh Balakrishnan

{"title":"Integrated statistical and machine-learning optimization for enhanced heparosan production by Lactococcus lactis.","authors":"Siddharth Guhan, Thilakraj S, Senthilkumar Sivaprakasam, Rengesh Balakrishnan","doi":"10.1080/10826068.2026.2703129","DOIUrl":"https://doi.org/10.1080/10826068.2026.2703129","url":null,"abstract":"Lactococcus lactis SH6 was engineered for heterologous heparosan production, and its growth medium was optimized using a combination of experimental design and machine learning (ML). One-factor-at-a-time shake flask experiments revealed glucose (10 g/L) and yeast extract (17.5 g/L) as the best substrates, producing 50 mg/L heparosan. Plackett-Burman analysis and steepest ascent optimization revealed significant factors, and a central composite design (CCD) optimized nutrient concentrations, predicting 85 mg/L heparosan (validated at 81 mg/L). ML-Gaussian process regression was applied after CCD optimization to fine-tune and cross-check the optimal medium (glucose 8.94 g/L, yeast extract 22.89 g/L, ascorbate 0.38 g/L, β-glycerophosphate 28.2 g/L), producing 85.28 mg/L heparosan (predicted 88.8 mg/L) at the flask scale. Earlier nisin induction (2 h) at the bioreactor scale increased heparosan titers to 119.7 mg/L, and linear glucose feeding (1.5 g/L.h) extended the production phase to 133 mg/L. Medium optimization resulted in nearly doubling heparosan yield compared to the unoptimized medium, setting a new standard for L. lactis. This work offers a design-of-experiments-ML solution as a viable approach to designing high-yielding, animal-product-free heparosan production methods in a Generally Regarded as Safe (GRAS) microbe.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148472499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid design, cloning, and expression of Vitreoscilla hemoglobin in Streptomyces pilosus: a novel strategy to enhance desferrioxamine B production and yield. 猪链霉菌中玻璃体振荡菌血红蛋白的质粒设计、克隆和表达：提高去铁胺B生产和产量的新策略。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-15 DOI: 10.1080/10826068.2026.2684610

Mahsa Arkani, Valiollah Babaeipour, Ali Asghar Deldar, Reza Mohammadi, Shadi Mosleh Moghadam

{"title":"Plasmid design, cloning, and expression of Vitreoscilla hemoglobin in Streptomyces pilosus: a novel strategy to enhance desferrioxamine B production and yield.","authors":"Mahsa Arkani, Valiollah Babaeipour, Ali Asghar Deldar, Reza Mohammadi, Shadi Mosleh Moghadam","doi":"10.1080/10826068.2026.2684610","DOIUrl":"https://doi.org/10.1080/10826068.2026.2684610","url":null,"abstract":"Streptomyces pilosus is the primary producer of desferrioxamine B (DFOB), to treat acute and chronic iron overload. Its growth and productivity depend on dissolved oxygen concentration. Vitreoscilla hemoglobin (VHb enhances oxygen transfer and metabolic efficiency under oxygen-limited conditions. In this study, the vgb gene was cloned into S. pilosus to enhance DFOB production. The shuttle vector pGM1192 was designed in SnapGene, synthesized, and propagated in E. coli TOP10. PCR and restriction digestion with NdeI and BamHI confirmed recombinant plasmids. The recombinant plasmid was subsequently transferred into S. pilosus via electroporation. Transformation was verified through colony PCR and electrophoresis on a 1% agarose gel. The kinetics of cell growth and DFOB production in the recombinant strain expressing VHb were analyzed and compared with those of the wild-type strain. The recombinant VHb-expressing strain showed improved growth and DFOB production relative to the wild-type strain, achieving an OD600 of approximately 11 and producing about 1.2 g/L DFOB within 24 h. In contrast, under identical conditions, the wild-type strain reached only an OD600 of about 5.5 and produced approximately 0.4 g/L DFOB. These results indicate that VHb-mediated oxygen enhancement represents an effective metabolic engineering strategy for boosting DFOB biosynthesis in S. pilosus.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.2,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148448695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of bacterial nanocellulose by Bacillus subtilis using combined mutagenesis and response surface optimization approaches. 利用诱变和响应面优化相结合的方法提高枯草芽孢杆菌的细菌纳米纤维素产量。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-15 DOI: 10.1080/10826068.2026.2701132

Lakshmi Gollapalli, Ranga Rao Ambati

{"title":"Enhanced production of bacterial nanocellulose by Bacillus subtilis using combined mutagenesis and response surface optimization approaches.","authors":"Lakshmi Gollapalli, Ranga Rao Ambati","doi":"10.1080/10826068.2026.2701132","DOIUrl":"https://doi.org/10.1080/10826068.2026.2701132","url":null,"abstract":"In the present study, a native Bacillus subtilis isolate-8 was subjected to sequential mutagenesis using ultraviolet (UV) irradiation, nitrous acid (HNO2) and N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to obtain enhanced BNC production by following mutagenesis steps, with maximum yields of 4.3 g/L, 8.1 g/L and 9.8 g/L achieved by the superior UV mutant (BCUV-08), nitrous acid mutant (BCN-06), and NTG mutant (BCNT-03), respectively. The hyperproducing mutant BCNT-03 was further optimized using response surface methodology (RSM) based on a central composite rotatable design (CCRD). Four critical process variables, namely inoculum size (0.5-2.5%, v/v), incubation temperature (20-40 °C), incubation period (5-25 days), and flask-to-medium ratio (1:6-1:2, v/v), were evaluated under static fermentation conditions using modified Hestrin-Schramm (HS) medium containing molasses as a low-cost carbon source. The developed quadratic model was highly significant (F = 16.27, p < 0.001) and explained 93.44% of the variation in BNC production (R2=0.9344). The optimum conditions predicted by the model corresponded to an inoculum size of 1.5% (v/v), incubation temperature of 30 °C, incubation period of 15 days, and flask-to-medium ratio of 1:4, resulting in a maximum BNC yield of 15.92 g/L. The combined mutagenesis and process optimization strategy substantially enhanced BNC production. The findings demonstrate the potential of B. subtilis as an alternative BNC-producing organism.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of poly (3-hydroxybutyrate) production by recombinant Escherichia coli using an experimental screening design. 利用实验筛选设计优化重组大肠杆菌生产聚（3-羟基丁酸酯）。

IF 2.2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-07-12 DOI: 10.1080/10826068.2026.2701137

Aleyna Filizfidanoglu, Tugbay-Sıla Altac, Yunus Ensari, Irem Deniz

{"title":"Optimization of poly (3-hydroxybutyrate) production by recombinant Escherichia coli using an experimental screening design.","authors":"Aleyna Filizfidanoglu, Tugbay-Sıla Altac, Yunus Ensari, Irem Deniz","doi":"10.1080/10826068.2026.2701137","DOIUrl":"https://doi.org/10.1080/10826068.2026.2701137","url":null,"abstract":"The negative effects of synthetic plastics on ecosystems and human health have increased interest in environmentally friendly and biodegradable bioplastics. Among bioplastics, polyhydroxyalkanoates (PHAs) stand out due to their biodegradability, biocompatibility, and thermal processability. Thus, there are several studies focused on bioplastics production with a high yield by using agricultural wastes as carbon source in order to meet the industrial needs. This study aimed to produce poly (3-hydroxybutyrate) (P3HB), one of the PHAs, by the recombinant Escherichia coli Rosetta pQEAL1124 strain using dried raisin waste (RW) as a carbon source. The optimization of physicochemical parameters affecting the production process were identified. For the optimization of P3HB production, conditions providing the maximum predicted response and a maximum desirability value of 0.86 were found to be 7.03 g/L RW, 2.46 g/L tryptone, and 194 rpm. The maximum P3HB yield was achieved as 490 mg/L under these conditions with a purity level of 86%. The Young's modulus value of the extracted P3HB was found to be 170 MPa. This study provides a valuable contribution to sustainable waste valorization and P3HB production by presenting an innovative perspective on environmentally friendly and sustainable production processes.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.2,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0