Preparative Biochemistry & Biotechnology最新文献

Sustainable production of 2-phenylethanol by flower-isolated yeasts using agro-industrial wastes as feedstock. 以农业工业废料为原料的花分离酵母可持续生产2-苯乙醇。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-05-05 DOI: 10.1080/10826068.2026.2667926

Stefany Kell Bressan, Anderson Giehl, Aline Perin Dresch, Aline Ruth Schmidt, Elisa Amorim Amâncio Teixeira, Odinei Fogolari, Angela Alves Dos Santos, João Paulo Bender, Carlos Augusto Rosa, Helen Treichel, Sérgio Luiz Alves

{"title":"Sustainable production of 2-phenylethanol by flower-isolated yeasts using agro-industrial wastes as feedstock.","authors":"Stefany Kell Bressan, Anderson Giehl, Aline Perin Dresch, Aline Ruth Schmidt, Elisa Amorim Amâncio Teixeira, Odinei Fogolari, Angela Alves Dos Santos, João Paulo Bender, Carlos Augusto Rosa, Helen Treichel, Sérgio Luiz Alves","doi":"10.1080/10826068.2026.2667926","DOIUrl":"https://doi.org/10.1080/10826068.2026.2667926","url":null,"abstract":"2-Phenylethanol (2-PE) is a commercially valuable aromatic alcohol, yet its sustainable bioproduction remains challenging due to low yields and high costs. This study initially analyzed the volatile organic compounds (VOCs) of eleven yeasts isolated from Senna macranthera nectaries. The resulting metabolic profiling revealed that 2-PE was the most prevalent volatile compound produced, reaching relative peak areas of up to 55%. This distinct prevalence drove the selection of Aureobasidium leucospermi CHAP-214 for optimization in cost-effective media composed of orange peel and malt bagasse extracts. While nitrogen supplementation with urea suppressed production and ammonium sulfate favored biomass over synthesis, the addition of L-phenylalanine (L-Phe) proved crucial. Specifically, the medium containing 4 g/L of L-Phe without extra nitrogen (Medium E3) achieved the optimal metabolic balance, yielding a maximum 2-PE concentration of 2.477 g/L with a high bioconversion efficiency of 0.83 g of 2-PE produced per g L-Phe consumed. Higher L-Phe levels (9 g/L) led to near-complete consumption but lower yields, indicating metabolic diversion. The addition of ammonium sulfate further reduced 2-PE formation, favoring biomass production instead. This is the first study to report the strong potential of A. leucospermi for 2-PE production using the novel combination of orange peel and malt bagasse extracts.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":1.9,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled vs. non-controlled culture systems for SARS-CoV-2 VLP production using the baculovirus/SF9 platform. 使用杆状病毒/SF9平台生产SARS-CoV-2 VLP的受控与非受控培养系统。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-05-05 DOI: 10.1080/10826068.2026.2661959

Luis Giovani de Oliveira Guardalini, Felipe Moura Dias, Thaissa Consoni Bernardino, Jaci Leme, Henrique Coelho de Oliveira, Aldo Tonso, Eutimio Gustavo Fernández-Núñez, Soraia Attie Calil Jorge

{"title":"Controlled vs. non-controlled culture systems for SARS-CoV-2 VLP production using the baculovirus/SF9 platform.","authors":"Luis Giovani de Oliveira Guardalini, Felipe Moura Dias, Thaissa Consoni Bernardino, Jaci Leme, Henrique Coelho de Oliveira, Aldo Tonso, Eutimio Gustavo Fernández-Núñez, Soraia Attie Calil Jorge","doi":"10.1080/10826068.2026.2661959","DOIUrl":"https://doi.org/10.1080/10826068.2026.2661959","url":null,"abstract":"COVID-19 is an infectious disease resulting from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged as a global pandemic in 2020. Virus-like particles (VLP) are artificially generated nanoparticles composed of a subset of viral components that closely mimic the native virus in size, structure, and surface composition. These features contribute to their robust immunogenic potential, stimulating both antibody-mediated and cell-mediated immune responses. This study aimed to evaluate the expression of SARS-CoV-2 structural proteins and the VLP production using a baculovirus-insect cell system. Two monocistronic recombinant baculoviruses, each carrying either the spike (S) or nucleocapsid (N) gene of SARS-CoV-2, were employed in two different culture systems: Schott flasks and a stirred-tank bioreactor. The Sf9 cells showed substantial differences between the Schott flask and stirred tank bioreactor in terms of metabolism, virus titer, and cell death after infection. Both proteins' expressions were confirmed in the two culture systems assessed. However, VLP formation (69.1-78.2 nm) was only confirmed in the co-infection setup with both monocistronic recombinant baculoviruses. In contrast, infection with only the S-protein baculovirus failed to produce VLP in our findings.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":1.9,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial and antioxidant activity of green synthesized copper nanoparticles. 绿色合成纳米铜的抗菌和抗氧化活性。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-29 DOI: 10.1080/10826068.2026.2661962

Suresh Chand Mali, Anita Dhaka, Sheetal Sharma, Rohini Trivedi

引用次数: 0

Litsea cubeba essential oil: phytochemistry, bioactivity-driven applications and extraction. 山苍子精油：植物化学，生物活性驱动的应用和提取。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-23 DOI: 10.1080/10826068.2026.2647206

Xiaodong Guo, Xiaojie Yan, Qingshan Shi, Xinyan Gao, Meize Jiang, Yueyuan Chen, Zhifang Cui

{"title":"Litsea cubeba essential oil: phytochemistry, bioactivity-driven applications and extraction.","authors":"Xiaodong Guo, Xiaojie Yan, Qingshan Shi, Xinyan Gao, Meize Jiang, Yueyuan Chen, Zhifang Cui","doi":"10.1080/10826068.2026.2647206","DOIUrl":"https://doi.org/10.1080/10826068.2026.2647206","url":null,"abstract":"This review provides a comprehensive overview of Litsea cubeba essential oil (EO), covering its extraction methods, compositional variation, bioactivities, and applications. It systematically examines both traditional (e.g., hydrodistillation, steam distillation) and emerging extraction techniques (e.g., ultrasound-, microwave-, enzyme-, and deep eutectic solvent-assisted methods), highlighting their respective advantages and limitations. A key focus is placed on how multiple factors-including geographical origin, harvest season, plant part, and extraction method-interact to influence the EO chemical profile and yield, which in turn determine its bioactivities. The growing market demand across food, pharmaceutical, daily chemical, and agrochemical sectors is discussed, with particular emphasis on how bioactivity-driven mechanisms (e.g., antioxidant, antimicrobial, and anti-inflammatory effects) underpin its diverse applications. While novel techniques offer improved efficiency, the review critically notes that methods using organic solvents or supercritical fluids may overestimate yield by co-extracting nonvolatiles. Moreover, a broader evaluation framework incorporating extraction efficiency, economic viability, and environmental impact is proposed. To promote resource-efficient utilization, a cascaded strategy-such as recovering kernel oils for biodiesel after EO distillation-is recommended for sustainable development. Finally, future interdisciplinary research should prioritize elucidating bioactivity mechanisms including synergistic/antagonistic interactions among EO components, enhancing formulation stability, and advancing high-purity citral separation technologies for value-added applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-17"},"PeriodicalIF":1.9,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiling functional metabolites and minerals of fortified food black gram Vigna mungo (L.) hepper. 强化食品黑克芒果（L.） hepper的功能代谢物和矿物质分析。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-23 DOI: 10.1080/10826068.2026.2647214

Smruti B Bhatt, Jaspreet Singh, Nagasathiya K, Vinduja Vasudevan, Saiprahalad Mani, Gargi Borkar, Harini P, Abinaya Kanagaraja, Subash C B Gopinath, Pachaiappan Raman

{"title":"Profiling functional metabolites and minerals of fortified food black gram Vigna mungo (L.) hepper.","authors":"Smruti B Bhatt, Jaspreet Singh, Nagasathiya K, Vinduja Vasudevan, Saiprahalad Mani, Gargi Borkar, Harini P, Abinaya Kanagaraja, Subash C B Gopinath, Pachaiappan Raman","doi":"10.1080/10826068.2026.2647214","DOIUrl":"https://doi.org/10.1080/10826068.2026.2647214","url":null,"abstract":"This study is focused on analyzing and profiling the compounds from the fortified food of black gram and its ingredients. The metabolites and minerals with its oxides were studied using GC-MS, HPLC, LC-MS/MS, XRD, FTIR and ICP-OES. This study provides the first integrated biochemical, proteomic, and mineral characterization of Ulutham kali, highlighting its nutritional value and functional food potential. From this study, it is revealed that the Ulutham kali contain fatty acids and their esters, carbohydrates and their derivatives, prenol lipids, flavonoids, vitamins, and glycerolipids were identified by GC-MS. Totally fifteen amino acids were quantified by HPLC including all three eight essential amino acids. The LC-MS/MS based peptide mass fingerprinting revealed the malate dehydrogenase, cationic peroxidases, and subtilisin precursor proteins in food formulation. The presence of potassium and melilite (Ca, Na)2[SiO7] complex were analyzed by XRD and the food formulation contains the functional groups such amines, amides, carboxylic acid, ketones, aldehydes, and esters by FTIR analysis. The minerals like calcium, iron, potassium, magnesium, manganese, sodium, phosphorus, sulfur, selenium, and zinc were quantified by ICP-OES. Also, the higher free radical scavenging activity of 52 % was recorded in the methanolic extract of black gram pudding.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":1.9,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic phytohormonal stimulation for enhanced carotenogenesis in Dunaliella salina. 协同植物激素刺激促进盐杜氏藻胡萝卜素生成。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-21 DOI: 10.1080/10826068.2026.2657385

Aswini Velmurugan, Balajii Muthusamy, Srinivasan Ramachandran, Nalini Easwaran, Gothandam Kodiveri Muthukaliannan

{"title":"Synergistic phytohormonal stimulation for enhanced carotenogenesis in Dunaliella salina.","authors":"Aswini Velmurugan, Balajii Muthusamy, Srinivasan Ramachandran, Nalini Easwaran, Gothandam Kodiveri Muthukaliannan","doi":"10.1080/10826068.2026.2657385","DOIUrl":"https://doi.org/10.1080/10826068.2026.2657385","url":null,"abstract":"The unicellular green microalga Dunaliella salina is well known for being one of the best natural sources of β-carotene, a potent antioxidant and essential precursor to vitamin A. β-carotene is vital for human health because it improves immune system performance, supports vision, and lowers the risk of a number of chronic illnesses, including some forms of cancer. So far, a number of methods to increase β-carotene production in D. salina, including genetic engineering, applying abiotic stressors, plant growth regulators, and using metal nanoparticles. In this study, we examined the synergistic effects of plant growth regulators on the production of β-carotene and biomass accumulation in D. salina during various growth phases. Among the phytohormones examined, methyl jasmonate (MeJA) and indole acetic acid (IAA) were discovered to have important physiological functions in controlling the microalga's growth and metabolism. We maximized productivity by optimizing these phytohormone concentrations using response surface methodology (RSM) with a central composite design (CCD). Our findings showed that when applied during the combination of 3 mg L-1 IAA implemented at non carotenogenic phase (the 0th day of cultivation), and 200 μM MeJA implemented on carotenogenic phase (20th day of the cultivation) significantly increased growth and carotenoid accumulation, yielding 5.2 g/L of biomass and a 2027.96 μg of β-carotene content for every 100 mg of dry cell weight.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":1.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduction and denaturation before affinity chromatography for host cell protein removal in aggregate-rich monoclonal antibody. 富聚合体单克隆抗体亲和层析去除宿主细胞蛋白前的还原和变性。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-21 DOI: 10.1080/10826068.2026.2652405

Tianwei Huang, Changrui Zou, Guiping Qi, Zhipeng Zhao, Yichen Zhang, Sean Xiangyuan He, Kaisheng Huang

{"title":"Reduction and denaturation before affinity chromatography for host cell protein removal in aggregate-rich monoclonal antibody.","authors":"Tianwei Huang, Changrui Zou, Guiping Qi, Zhipeng Zhao, Yichen Zhang, Sean Xiangyuan He, Kaisheng Huang","doi":"10.1080/10826068.2026.2652405","DOIUrl":"https://doi.org/10.1080/10826068.2026.2652405","url":null,"abstract":"Host cell proteins (HCPs) are important process-related safety concern factors during drug development, clinical trials and manufacturing, and their removal becomes particularly challenging when associated with antibody aggregates. Residual HCPs in drug products could trigger adverse effects or compromise drug efficacy and stability. This study aims to enhance the HCP removal specifically in monoclonal antibodies (mAbs) with high aggregate content before protein A chromatography by introducing reduction and denaturation steps. A model mAb was purified by different processes, and the impact on HCP removal was evaluated by enzyme-linked immunosorbent assay (ELISA) and Liquid chromatography-mass spectrometry (LC-MS). The HCP profiles in high aggregate, low aggregate and monomeric mAbs were analyzed by LC-MS. The additional reduction and denaturation steps improved HCP removal rate by 1.08 log10 reduction value (LRV) compared to the control process. For the relatively abundant high-risk HCPs, 45% were completely removed. HCP removal in the model mAb was significantly enhanced, especially in high aggregate forms. This method can reduce HCP carry-over from mAbs aggregates in subsequent downstream processing and can provide an efficient HCP removal strategy for manufacturing to improve patient safety and drug stability.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":1.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yogurt production with GABA-producing cultures: physicochemical properties and GABA content. 用产生GABA的培养物生产酸奶：理化性质和GABA含量。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-21 DOI: 10.1080/10826068.2026.2658170

Gokturk Erikli, Sefa Isik, Selma Kayacan Cakmakoglu, Zeynep Hazal Tekin Cakmak, Enes Dertli

{"title":"Yogurt production with GABA-producing cultures: physicochemical properties and GABA content.","authors":"Gokturk Erikli, Sefa Isik, Selma Kayacan Cakmakoglu, Zeynep Hazal Tekin Cakmak, Enes Dertli","doi":"10.1080/10826068.2026.2658170","DOIUrl":"https://doi.org/10.1080/10826068.2026.2658170","url":null,"abstract":"In this study, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains isolated from traditional yogurts in Türkiye were screened for their gamma-aminobutyric acid (GABA) production potential. High GABA-producing strains were selected and used to develop GABA-enriched yogurt through monosodium glutamate (MSG) supplementation. It was determined that GABA content increased in yogurts produced with MSG addition compared to the control. While GABA levels in the control group ranged between 0.07-0.10 mg mL-1 during storage, they were found to be between 0.95 and 1.19 mg mL-1 in MSG-supplemented yogurts. Titratable acidity and pH values differed from the control throughout the storage period. Water-holding capacity and syneresis results were not statistically different compared to the control. Improvements in textural properties were observed at the end of the storage period. According to these results, the selected starter culture combination and MSG addition are an effective strategy for producing GABA-enriched yogurt. These findings support the development of functional dairy products with enhanced bioactive potential.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":1.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosurfactant production by Pseudomonas aeruginosa WARP_W1: kinetics and structural elucidation of glycolipids. 铜绿假单胞菌WARP_W1生产生物表面活性剂：糖脂的动力学和结构解析。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-17 DOI: 10.1080/10826068.2026.2658165

Roshan Jaiswal, Padmanaban Velayudhaperumal Chellam

{"title":"Biosurfactant production by Pseudomonas aeruginosa WARP_W1: kinetics and structural elucidation of glycolipids.","authors":"Roshan Jaiswal, Padmanaban Velayudhaperumal Chellam","doi":"10.1080/10826068.2026.2658165","DOIUrl":"https://doi.org/10.1080/10826068.2026.2658165","url":null,"abstract":"In this study, biosurfactant-producing bacterial isolates were screened and isolated from a hydrocarbon-rich automobile workshop, marine water, agarwood, and ayurvedic industrial waste. The efficient bacterial isolate Pseudomonas aeruginosa WARP_W1 reduced surface tension to 35.21 mN/m and emulsified 64.32% of olive oil. The biosurfactant production was attempted using different oil sources, with coconut oil producing 899.69 mg/L biosurfactant, which is 35.8% more than olive oil. Logistic kinetic models accurately predicted microbial growth rate (R2 > 0.975) and biosurfactant production rate at 0.348h-1 and 0.201h-1, respectively. These data suggest that coconut oil could be a suitable substrate for biosurfactant. The physicochemical properties were also found to be efficient, with a low critical micelle concentration of 110.60 mg/L and a lowered surface tension of 26.99 mN/m in coconut oil. FTIR and NMR spectroscopy confirmed the glycolipid in the produced biosurfactant by showing rhamnolipid-like structural features. P. aeruginosa WARP_W1 is ideal for large-scale biosurfactant production because of its versatile utilization of carbon sources. This study provides the growth and product kinetic information on the substrate-specific production of biosurfactants.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":1.9,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-bio interface modulation using Garcinia spicata-capped silver nanoparticles (GS-AgNPs) for combating multidrug-resistant bacteria. 利用藤黄盖银纳米颗粒（GS-AgNPs）调制纳米生物界面以对抗多重耐药细菌。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-04-17 DOI: 10.1080/10826068.2026.2658178

Palaniyandi Velusamy, Govindarajan Venkat Kumar, Eman A Alabdullkarem, Ebenezar Immanuel J, Elumalai Pandian, Subash C B Gopinath

{"title":"Nano-bio interface modulation using Garcinia spicata-capped silver nanoparticles (GS-AgNPs) for combating multidrug-resistant bacteria.","authors":"Palaniyandi Velusamy, Govindarajan Venkat Kumar, Eman A Alabdullkarem, Ebenezar Immanuel J, Elumalai Pandian, Subash C B Gopinath","doi":"10.1080/10826068.2026.2658178","DOIUrl":"https://doi.org/10.1080/10826068.2026.2658178","url":null,"abstract":"The aim of this study was to synthesize silver nanoparticles (AgNPs) using the leaf extract of Garcinia spicata (GS). The biosynthesized GS-AgNPs were confirmed by the appearance of a characteristic surface Plasmon resonance (SPR) peak at 420 nm using UV-Vis spectroscopy. Further, the morphology, biomolecular capping, and crystalline structure of GS-AgNPs were carried out using advanced analytical techniques such as X-ray diffraction (XRD) characterization, Fourier transform-infrared (FTIR) and field emission-scanning electron microscopy (FESEM). The FESEM images exhibited a spherical shape with diameter in the range of 30-60 nm. These nanoparticles exhibited significant antimicrobial activity toward multi-drugs resistant bacterial pathogens of Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa at a concentration of 12 µg/mL. Furthermore, their anti-inflammatory properties were evaluated through protein-denaturation, membrane stabilization and nitric oxide scavenging assays, all of which demonstrated notable inhibition of inflammatory mediators. These findings suggest potential of GS as a promising and sustainable source for the phyto-assisted synthesis of GS-AgNPs with potential applications in antibacterial and anti-inflammatory therapies. Based on available information, this work is the first to report the greener synthesis of GS-AgNPs using Garcinia spicata leaf extracts.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":1.9,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0