Preparative Biochemistry & Biotechnology最新文献

Development of a scalable semi-synthetic medium for high-yield diphtheria toxin production using a definitive screening design an innovative statistical optimization method.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-21 DOI: 10.1080/10826068.2025.2465991

Prashant R Chawla, Uma Addepally

{"title":"Development of a scalable semi-synthetic medium for high-yield diphtheria toxin production using a definitive screening design an innovative statistical optimization method.","authors":"Prashant R Chawla, Uma Addepally","doi":"10.1080/10826068.2025.2465991","DOIUrl":"https://doi.org/10.1080/10826068.2025.2465991","url":null,"abstract":"Corynebacterium diphtheriae is the causative agent of diphtheria, which continues to be a serious health risk to children, particularly in countries such as India. Immunization is the best way to fight this illness. Enhancing the synthesis of diphtheria toxin (DT) is essential for the production of vaccines, particularly as immunization programs advance. Pork digestion medium (PDM) was employed as the standard medium for DT production. Nevertheless, this medium has issues with contamination and batch-to-batch variation. The production of DT is extremely low in the alternative synthetic medium. Compared to synthetic media, semi-synthetic media exhibit superior performance. This study's current goal was to increase DT production through the use of the Definitive Screening Design (DSD) methodology to optimize the composition of semi-synthetic media. A total of 11 components were selected for screening of the best suitable components for DT production. NZ-amine, tryptone N1, and maltose had the highest effects on DT production out of all the nutrients that were chosen. The model accuracy is indicated by the R2 value of 0.9820, which enables the prediction of DT yields. The model suggests the lower concentrations of NZ-amine combined with the moderate amounts of maltose and tryptone N1 is best suitable for the higher amounts of DT yields. With optimized conditions 174 Lf/mL of DT yield was achieved in validation experiments, which is nearer to the PDM yields. According to this study, this enhanced technique, which makes use of an affordable and expandable medium, could make large-scale toxoid production feasible.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of proteases for animal feed supplementation: scientific and technological updates.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-20 DOI: 10.1080/10826068.2025.2465957

Tiago de Oliveira Sousa, Neurimar Araújo da Silva, Vagne de Melo Oliveira, Alan Vagner da Silva Ramos, José Pedro Martins Barbosa Filho, Juanize Matias da Silva Batista, Romero Marcos Pedrosa Brandão Costa, Ana Lúcia Figueiredo Porto, Stélio Bezerra Pinheiro de Lima, Maurício de Paula Ferreira Teixeira, Thiago Pajeú Nascimento

{"title":"Use of proteases for animal feed supplementation: scientific and technological updates.","authors":"Tiago de Oliveira Sousa, Neurimar Araújo da Silva, Vagne de Melo Oliveira, Alan Vagner da Silva Ramos, José Pedro Martins Barbosa Filho, Juanize Matias da Silva Batista, Romero Marcos Pedrosa Brandão Costa, Ana Lúcia Figueiredo Porto, Stélio Bezerra Pinheiro de Lima, Maurício de Paula Ferreira Teixeira, Thiago Pajeú Nascimento","doi":"10.1080/10826068.2025.2465957","DOIUrl":"https://doi.org/10.1080/10826068.2025.2465957","url":null,"abstract":"The global market is rapidly evolving, with biotechnological advances supporting production centers through innovative techniques. A key focus is on bioprocesses for extracting, purifying, and applying proteases, particularly in animal feed within the agricultural sector. Although the cost reduction may vary depending on the animal species, supplementation with proteases allows for the formulation of more cost-effective diets, improving nutrient digestibility and absorption, optimizing energy efficiency, preserving intestinal integrity, enhancing carcass quality, modulating the intestinal microbiome, and reducing nitrogen excretion. This study aimed to update on protease use in monogastric animal feed, drawing from digital databases (Science Direct, Google Scholar, and Scielo) and industrial property databases (INPI). Despite the significant potential of proteases in monogastric animal nutrition, there is a lack of studies, especially on patented technologies. While their benefits are recognized, substantial investments are needed to develop new technologies and identify alternative protease sources. These advancements could enhance animal performance and strengthen Brazil's productive sector, highlighting the need for further research and innovation. The literature review showed promising results, but research is mainly focused on microbial proteases for poultry, pigs, and fish. There is significant potential to explore diverse protease production sources and their application across various species.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From probiotic fermentation to functional drinks: a review on fruit juices with lactic acid bacteria and prebiotics.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-19 DOI: 10.1080/10826068.2025.2467441

Nurhazwani Sa'aid, Joo Shun Tan

{"title":"From probiotic fermentation to functional drinks: a review on fruit juices with lactic acid bacteria and prebiotics.","authors":"Nurhazwani Sa'aid, Joo Shun Tan","doi":"10.1080/10826068.2025.2467441","DOIUrl":"https://doi.org/10.1080/10826068.2025.2467441","url":null,"abstract":"In recent years, the demand for probiotic beverages has surged, with dairy products traditionally serving as the primary sources of probiotics. However, many consumers face health issues such as lactose intolerance, milk allergies, and high cholesterol, which prevent them from consuming dairy products. This has led to the exploration of nondairy alternatives, particularly fruit juices, as carriers for probiotics. Lactic acid bacteria (LAB) have been identified as beneficial probiotics that can be incorporated into these beverages. The inclusion of prebiotics, such as inulin and galacto-oligosaccharides (GOS), in fruit juices has shown promise in enhancing the growth and activity of LAB, thereby creating functional beverages that support digestive health. Despite numerous studies on fruit juice fermentation, there is limited data on the optimal pairing of probiotics and prebiotics to develop stable, nondairy functional drinks. This review underscores the potential of lactic acid fermentation and the integration of prebiotics and probiotics in fruit juices, highlighting the necessity for further research to optimize these combinations for enhanced health benefits and improved beverage stability.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-20"},"PeriodicalIF":2.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized extraction of Spirulina platensis phenolics using natural deep eutectic solvents for cosmetics.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-19 DOI: 10.1080/10826068.2025.2467440

Semra Nur Kamil, Selin Tezcanlı, Yaren Çelik, İrem Toprakçı, Selin Şahin

{"title":"Optimized extraction of Spirulina platensis phenolics using natural deep eutectic solvents for cosmetics.","authors":"Semra Nur Kamil, Selin Tezcanlı, Yaren Çelik, İrem Toprakçı, Selin Şahin","doi":"10.1080/10826068.2025.2467440","DOIUrl":"https://doi.org/10.1080/10826068.2025.2467440","url":null,"abstract":"This study presents a highly environmentally friendly system using natural deep eutectic solvent (NADES) as an organic solvent substitute to obtain high-quality microalgae extract, which is a potential new source of moisturizing cosmetic materials. Blue-green algae Spirulina platensis was selected as the alg metabolite source. Choline chloride and glycerol were used as the ingredients of the NADES owing to they are commonly used in cosmetic products for skin moisturizing and/or healing. The extraction of bioactives from Spirulina platensis was optimized based on the multi-response optimizition of Response Surface method (RSM). 3-factor and 3-level Box-Behnken design was implemented for the design matrix with 17 experimental runs. The best conditions were attained by almost 21% (v/v) water addition into the best NADES (choline chloride/glycerol, ½ molar ratio) for 30 sec of extraction time under almost 7500 rpm mixing speed. The given conditions provided the highest yields of total phenolic content (10.517 mg-GAE/g-DM), antioxidant activity (1.796 mg-TEAC/g-DM) and 1,2,3,4-butanetetrol (70.174%), which were approved with satisfactory validation findings (the error < 2%). The application of the produced products gave promising findings for the valorization of the NADES-based alg metabolites as potential natural additives used directly in cosmetic formulations.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882 from waste tobacco leaves.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-18 DOI: 10.1080/10826068.2025.2467446

Jinchu Yang, Yongfeng Yang, Zhenzhen Huang, Zongcan Yang, Xiaolong Chen, Haoliang Li, Yi Zheng, Gangchun Sun, Renyong Zhao, Wen-Wen Zhou, Zichao Wang, Shouai Feng, Qiuling Wang

{"title":"Structural characterization and antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882 from waste tobacco leaves.","authors":"Jinchu Yang, Yongfeng Yang, Zhenzhen Huang, Zongcan Yang, Xiaolong Chen, Haoliang Li, Yi Zheng, Gangchun Sun, Renyong Zhao, Wen-Wen Zhou, Zichao Wang, Shouai Feng, Qiuling Wang","doi":"10.1080/10826068.2025.2467446","DOIUrl":"https://doi.org/10.1080/10826068.2025.2467446","url":null,"abstract":"Due to the restricts of treatment methods and high costs, waste tobacco materials are usually discarded, which leads to resources waste and environmental pollution. Present work used waste tobacco leaves as carbon source to produce polysaccharide (CGP-TL) by Chaetomium globosum CGMCC 6882 via submerged fermentation. CGP-TL yield was 1.62 ± 0.35 g/L, and the contents of carbohydrate and protein in which were 91.73% ± 6.49% and 5.51% ± 0.84%. Structural characterization showed CGP-TL was composed of rhamnose, arabinose, galactose, glucose, mannose and xylose in a molar ratio of 9.9688: 6.8482: 19.6045: 29.4448: 0.8033: 0.5874. Meanwhile, its weight-average molecular weight, number-average molecular weight and polydispersity were 286.035 kDa, 118.146 kDa and 2.421, respectively. In vitro antioxidant analysis showed CGP-TL scavenged DPPH, ABTS, hydroxyl and superoxide radicals positively correlated to its concentration, and the corresponding scavenging effects at 2.5 mg/mL were 75.53% ± 1.89%, 96.24% ± 2.32%, 52.91% ± 2.28% and 65.26% ± 2.47%. Furthermore, CGP-TL not only improved the antioxidative status of RAW 264.7 cells, but also exerted oxidative repairing effects on H2O2-damaged RAW 264.7 cells via increasing the levels of SOD, CAT and GSH-Px, and reducing MDA level. Overall, this work will provide references for green and efficient utilization of waste tobacco materials and cellulose resources.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of bioactive compounds extraction from Red Cardinal grape pomace using choline chloride and lactic acid-based deep eutectic solvent.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-18 DOI: 10.1080/10826068.2025.2465960

Thao Thi Thanh Nguyen, Viet Bao Nguyen, Tuyen Chan Kha

{"title":"Optimization of bioactive compounds extraction from Red Cardinal grape pomace using choline chloride and lactic acid-based deep eutectic solvent.","authors":"Thao Thi Thanh Nguyen, Viet Bao Nguyen, Tuyen Chan Kha","doi":"10.1080/10826068.2025.2465960","DOIUrl":"https://doi.org/10.1080/10826068.2025.2465960","url":null,"abstract":"Grape pomace, a major by-product of the wine and juice industries, presents considerable potential for extracting valuable bioactive compounds. This study aimed to optimize the extraction process using deep eutectic solvents composed of choline chloride and lactic acid. The extraction focused on three key factors: solvent-to-material ratio, extraction temperature, and extraction time, employing response surface methodology with a central composite design. The results showed that the optimal extraction conditions were achieved at a solvent-to-material ratio of 25:1 (v · w-1), an extraction temperature of 70 °C, and an extraction time of 118 min. Under these conditions, the extract exhibited high levels of total phenolic content (81.90 ± 1.06 mg GAE · g-1 DW), total saponin content (174.66 ± 2.87 mg AE · g-1 DW), and total proanthocyanidin content (31.13 ± 0.50 mg CE · g-1 DW). Furthermore, the extract demonstrated strong antioxidant capacity and antibacterial activities against four microbial strains (Escherichia coli ATCC 8739, Salmonella typhimurium ATCC 14028, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538). These findings underscore the extract's potential for diverse industrial applications, including the food, nutraceutical, and cosmetic sectors.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and in vitro activity evaluation of protein transduction domain-transactivator of transcription and Candida antarctica lipase B fusion proteins.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-12 DOI: 10.1080/10826068.2025.2460511

Xinhua Fu, Huawei Qin, Yuanqing Ma, Dongxia Li, Fujun Peng, Shuhui Zhang

{"title":"Construction and in vitro activity evaluation of protein transduction domain-transactivator of transcription and Candida antarctica lipase B fusion proteins.","authors":"Xinhua Fu, Huawei Qin, Yuanqing Ma, Dongxia Li, Fujun Peng, Shuhui Zhang","doi":"10.1080/10826068.2025.2460511","DOIUrl":"https://doi.org/10.1080/10826068.2025.2460511","url":null,"abstract":"Protein transduction domain (PTD)- transactivator of transcription (Tat) and the alkaline cold-active lipase Candida antarctica lipase B (calB) were used to construct a calB-Tat recombinant protein and examine its membrane-penetrating ability. The calB gene was fused with a PTD-Tat-encoding fragment to create the in-frame calB-Tat. After digestion with Nco I and Xho I, the calB-Tat fragment was subcloned and inserted into the expression vector pET28a. The recombinant plasmid pET28a-calB-Tat was subsequently transferred into E.coli Rosetta (DE3) cells to express the labeled protein calB-Tat. Protein concentrations were measured using a commercial BCA kit, and the transmembrane activity of the proteins in SH-SY5Y cells was observed under a fluorescence-inverted microscope. MTT and Western blotting assays were conducted to examine toxicity. The fusion protein exhibited low toxicity. As the concentration of the fusion protein decreased, the effect on cell viability decreased. Additionally, the fusion protein penetrated the cell membrane penetration was stable and was specifically expressed in cells. Taken together, the pET28a-calB-Tat prokaryotic vector was generated, yielding a significant amount of the calB-Tat protein. This increased the cell membrane and perhaps reveals a new way of delivering weight-loss drugs and protein-based medications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-7"},"PeriodicalIF":2.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile microwave-assisted green synthesis and characterization of flower shaped zinc oxide nanoclusters using Centella asiatica (Linn.) leaf extract and evaluation of its antimicrobial activity and in vivo toxic effects on Artemia nauplii.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-08 DOI: 10.1080/10826068.2025.2460498

Vipina Vinod T N, Amy S Mathew, Divya Mathew, Jyothis Mathew, Radhakrishnan E K

{"title":"Facile microwave-assisted green synthesis and characterization of flower shaped zinc oxide nanoclusters using Centella asiatica (Linn.) leaf extract and evaluation of its antimicrobial activity and in vivo toxic effects on Artemia nauplii.","authors":"Vipina Vinod T N, Amy S Mathew, Divya Mathew, Jyothis Mathew, Radhakrishnan E K","doi":"10.1080/10826068.2025.2460498","DOIUrl":"https://doi.org/10.1080/10826068.2025.2460498","url":null,"abstract":"Green synthesized nanomaterials play a vital role in nanotechnology was due to its diverse applications. In the current study, flower shaped nanoclusters of zinc oxide nanoparticles (ZnONPs) was fabricated using the leaf extract of Centella asiatica (Linn.) by microwave-assisted method. The physico-chemical characterization of the green synthesized ZnONPs were further conducted by the UV-Vis spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and transmission electron microscopy analysis. The UV-Vis spectrum of the synthesized ZnONPs has showed characteristic absorption maximum at 363 nm. The XRD pattern of the same could confirm to have the crystalline nature of ZnONPs. Additionally, the FT-IR spectra have revealed the presence of characteristic stretching and bending vibrations of the Zn-O bond, along with those of phytochemicals that might have involved in ZnONPs stabilization. By the HR-TEM imaging, agglomeration of the nanoparticles and thereby the formation of flower-like clusters could be observed. Furthermore, the synthesized ZnONPs have remarkable antimicrobial activity against S. aureus and E. coli with inhibition zones of 15 ± 0.4 and 16.5 ± 1.0 mm respectively. The green synthesized ZnONPs showed no significant toxicity toward Artemia nauplii. Hence, the results of the study indicate the promising potential of the synthesized ZnO nanoclusters.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced identification of glycoproteins and allergens in Hevea brasiliensis latex using innovative enrichment techniques.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-04 DOI: 10.1080/10826068.2025.2460520

Nur Syafiqah Mohd Afandi, Mohd Nazri Ismail

{"title":"Enhanced identification of glycoproteins and allergens in Hevea brasiliensis latex using innovative enrichment techniques.","authors":"Nur Syafiqah Mohd Afandi, Mohd Nazri Ismail","doi":"10.1080/10826068.2025.2460520","DOIUrl":"https://doi.org/10.1080/10826068.2025.2460520","url":null,"abstract":"Hevea brasiliensis is the most efficient crop for latex production and has been the main source for global commercialisation since 1876. However, little is known about its glycosylation properties. Studying glycoproteomics has been challenging due to analytical difficulties and the heterogeneous nature of glycans. In this study, we extracted natural rubber latex (NRL) proteins, purify peptide/glycopeptide mixtures and isolate glycopeptides using HILIC, SAX, and TiO2 enrichment methods. TiO2 showed high selectivity compared to SAX and HILIC, retaining unique proteins and glycans based on hydrophilicity. We identified proteins using LC-MS/MS and PEAKS Studio 7.5. These methods significantly improved protein coverage compared to non-enriched samples. Three glycoproteins (patatin, REF, and glucan endo-1,3-beta-D-glucosidase) and seven internationally recognised allergens (hev b 1, hev b 2, hev b 3, hev b 5, hev b 7, hev b 14, and hev b 15) were identified. These enrichment methods not only helped identify glycoproteins but also improved protein sequence coverage.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of process parameters for specific pathogen-free chicken embryonic fibroblast cultivation for yellow fever vaccine production. 优化用于黄热病疫苗生产的特异性无病原体鸡胚成纤维细胞培养的工艺参数。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-25 DOI: 10.1080/10826068.2024.2382795

Hareesh Reddy Narreddy, Ratna Prakash Kondapalli, Venkateswarulu Tc

{"title":"Optimization of process parameters for specific pathogen-free chicken embryonic fibroblast cultivation for yellow fever vaccine production.","authors":"Hareesh Reddy Narreddy, Ratna Prakash Kondapalli, Venkateswarulu Tc","doi":"10.1080/10826068.2024.2382795","DOIUrl":"10.1080/10826068.2024.2382795","url":null,"abstract":"The yellow fever (YF) vaccine is usually produced with egg-based methods, which has limitations, including potential adverse effects and low production yields. Alternatively, producing the vaccine using Vero cells or HEK 293 cells can overcome some of these issues, but these methods are significantly more expensive. In the current study, the YF vaccine candidate 17DD virus was produced in primary chicken embryo fibroblast (CEF) cells. The primary CEF cells isolation from eggs was optimized through a two-step process. In the first step, the important parameters that contribute to the development of the egg embryo, such as egg position, relative humidity (RH), and incubation time are optimized. In second step, primary CEF release parameters namely; trypsin volume and incubation temperature are optimized. Both steps were optimized using statistical methods. Further, the seeding cell density of isolated CEF was also optimized. It was observed that 5 x 104 cells/cm2 gave the highest virus titer of 3.89 million PFU/ml. The 17DD yields achieved in primary CEFs are much higher than egg-based production and it is an economically viable method.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"210-216"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0