Preparative Biochemistry & Biotechnology最新文献

Green extraction process optimization: extracting natural antioxidant active ingredients from Inonotus hispidus using RSM and GA-ANN modeling. 绿色提取工艺优化：采用RSM法和GA-ANN建模法，从芦笋中提取天然抗氧化活性成分。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-31 DOI: 10.1080/10826068.2025.2533448

Jingnan Li, Haiying Bao, Huimin Huo

{"title":"Green extraction process optimization: extracting natural antioxidant active ingredients from Inonotus hispidus using RSM and GA-ANN modeling.","authors":"Jingnan Li, Haiying Bao, Huimin Huo","doi":"10.1080/10826068.2025.2533448","DOIUrl":"https://doi.org/10.1080/10826068.2025.2533448","url":null,"abstract":"Inonotus hispidus (Bull.) P. Karst., a rare medicinal fungus, is rich in sterols and polyphenols, recognized for their natural antioxidant properties. To enhance its value, a sustainable extraction technique was developed, comparing the yields of Orbital Shaking Extraction, Hot Water Extraction, and Ultrasound-Assisted Extraction (UAE) under identical conditions. The results showed that UAE significantly outperformed the other methods. Response Surface Methodology (RSM) and Genetic Algorithm-Artificial Neural Network (GA-ANN) models were employed for optimizing the UAE process to maximize the extraction rates of the desired components. The GA-ANN model demonstrated superior predictive and optimization performance compared to RSM. The optimal conditions identified were an ultrasound time of 120 min, a liquid-to-material ratio of 22:1 mL/g, an ethanol concentration of 50%, and ultrasonic power of 63 W. Following optimization, the extraction rates of sterols and polyphenols increased by 171.92% and 69.56%, respectively, compared to the initial method. Antioxidant tests further indicated that the optimized extract exhibited enhanced free radical scavenging capabilities. These findings provide a theoretical foundation for the efficient and sustainable development of Inonotus hispidus and its industrial applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":1.9,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of biodegradable polyhydroxybutyrate PHB plastic through the combination of eco-friendly materials by Bacillus cereus B52. 蜡样芽孢杆菌B52与环保材料的结合，提高了可生物降解聚羟基丁酸酯PHB塑料的产量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-21 DOI: 10.1080/10826068.2025.2533443

Alfa Zulkarnain, Mulyorini Rahayuningsih, Ni Putu Ratna Ayu Krishanti, Fateha Fateha, Rossy Choreun Nissa, Kharis Yohan Abidin, Yeyen Nurhamiyah

{"title":"Enhanced production of biodegradable polyhydroxybutyrate PHB plastic through the combination of eco-friendly materials by Bacillus cereus B52.","authors":"Alfa Zulkarnain, Mulyorini Rahayuningsih, Ni Putu Ratna Ayu Krishanti, Fateha Fateha, Rossy Choreun Nissa, Kharis Yohan Abidin, Yeyen Nurhamiyah","doi":"10.1080/10826068.2025.2533443","DOIUrl":"https://doi.org/10.1080/10826068.2025.2533443","url":null,"abstract":"Polyhydroxybutyrate (PHB) is an environmentally friendly polymer that is synthesized by bacteria and stored as a carbon source. One of the main problems in PHB production is the price of materials for production. This study aims to optimize environmentally friendly and affordable materials such as molasses, tofu wastewater, and coconut water to increase PHB yield. Statistical optimization with central composite design (CCD) was used to optimize materials in Bacillus cereus B52 (B. cereus B52) for PHB production. The concentration of molasses, tofu wastewater and coconut water were used as optimization parameters to increase the PHB yield. The optimization results resulted in the production of PHB yield of 1.45 ± 0.14 g/L. Chemical analysis was used to determine the biopolymer validation of PHB. In conclusion, production optimization using a combination of materials from molasses, tofu wastewater, and coconut water can increase PHB production in B. cereus B52.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of silver nanoparticles by thermophilic spore-forming bacilli: screening for high-performance strains and characterization of silver nanoparticles from Anoxybacillus sp. D401a. 嗜热孢子形成杆菌合成纳米银的研究：高效菌株筛选及无氧芽孢杆菌D401a纳米银的表征

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-18 DOI: 10.1080/10826068.2025.2532470

Ayse Hilal Yalcinoz, Gaye Ekin Gursoy Calis, Basar Karaca, Hakan Eskizengin, Arzu Coleri Cihan

{"title":"Biosynthesis of silver nanoparticles by thermophilic spore-forming bacilli: screening for high-performance strains and characterization of silver nanoparticles from Anoxybacillus sp. D401a.","authors":"Ayse Hilal Yalcinoz, Gaye Ekin Gursoy Calis, Basar Karaca, Hakan Eskizengin, Arzu Coleri Cihan","doi":"10.1080/10826068.2025.2532470","DOIUrl":"https://doi.org/10.1080/10826068.2025.2532470","url":null,"abstract":"In this study, thermophilic, spore-forming bacteria from the families Bacillaceae and Anoxybacillaceae were analyzed for their ability to produce silver nanoparticles (AgNPs). Anoxybacillus sp. D401a was selected due to its high AgNP synthesis potential. The culture conditions (NaCl-free nutrient broth, 60 °C, pH 8.5, 48 hr) and synthesis parameters (1.5 mM AgNO3, 48 hr at 60 °C, pH 8.5) were optimized, resulting in a sevenfold increase in AgNP yield. Scale-up production yielded 139.4 mg of dry AgNPs with strong antimicrobial activity that exhibited significant minimum inhibitory concentration (MIC) values against Gram-positive, Gram-negative and fungal pathogens, outperforming commercial AgNPs. Sub-MIC concentrations of the biosynthesized AgNPs also inhibited biofilm formation and quorum sensing in Chromobacterium violaceum ATCC 12472. Characterization by ultraviolet-visible spectroscopy revealed a surface plasmon resonance peak at 400-450 nm, and Fourier-transform infrared spectroscopy indicated the presence of organic residues that stabilized the particles. Transmission electron microscopy analysis showed predominantly spherical AgNPs (24-57 nm). MTT assays showed a dose-dependent cytotoxicity against human keratinocytes (HaCaT), fibroblasts (HDF, L929), and cancer cells (HT-29, MCF-7), indicating a therapeutic window compared to commercial AgNPs. These results emphasize the potential of Anoxybacillus-derived AgNPs as a sustainable alternative for biomedical applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-23"},"PeriodicalIF":2.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and characterization of three alginate lyases from Vibrio sp. E: targeting kelp hydrolysis and biofilm disruption. 以海带水解和生物膜破坏为目标的三种海藻酸解酶的表达和特性研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-16 DOI: 10.1080/10826068.2025.2525209

Yu Wang, Xilei Xu, Wenqian Wu, Juan Gao, Yumei Li

引用次数: 0

Investigation of the effect of Streptomyces pilosus morphology and effective factors on desferrioxamine B production based on Taguchi experimental design. 基于田口试验设计的猪链霉菌形态及影响因素对去铁胺B产量的影响研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-10 DOI: 10.1080/10826068.2025.2525206

Shadi Mosleh Moghadam, Valiollah Babaeipour, Rasoul Khalilzadeh

{"title":"Investigation of the effect of Streptomyces pilosus morphology and effective factors on desferrioxamine B production based on Taguchi experimental design.","authors":"Shadi Mosleh Moghadam, Valiollah Babaeipour, Rasoul Khalilzadeh","doi":"10.1080/10826068.2025.2525206","DOIUrl":"https://doi.org/10.1080/10826068.2025.2525206","url":null,"abstract":"In this study, the desferrioxamine B (DFOB) production by Streptomyces pilosus was optimized. First, due to the diversity of bacterial morphology under different operating conditions and its significant effect on DFOB production, the cultivation conditions were optimized to obtain consistent and reproducible inoculum morphology. By examining the impact of four factors-flask type, pH, temperature, and stirring speed-at three levels using the Taguchi method, DFOB production improved from 0.64 to 0.79 g/L with a baffled shake flask, pH 7.3, 150 rpm stirring, and a temperature of 29 °C. Further investigation into culture medium composition, including the type and concentration of carbon source, lysine, and KH2PO4, using the L9 orthogonal array, enhanced DFOB production to 0.9 g/L under optimal conditions. A reduction in iron ion concentration of the culture medium significantly increased DFOB levels to 1.31 g/L. Investigating bacterial growth conditions and DFOB production under optimal conditions in a laboratory bioreactor, while reducing the production process time from 66 to 8 h and without significant changes in biomass, increased the DFOB concentration to 1.5 g/L, which is the highest DFOB production efficiency recorded to date.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process development of nattokinase microcapsules based on a novel sodium alginate-gelatin-chitosan composite material. 海藻酸钠-明胶-壳聚糖复合材料制备纳豆激酶微胶囊的工艺研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-10 DOI: 10.1080/10826068.2025.2528396

Junying Sun, Feifei Mao, Tongjun Liu

{"title":"Process development of nattokinase microcapsules based on a novel sodium alginate-gelatin-chitosan composite material.","authors":"Junying Sun, Feifei Mao, Tongjun Liu","doi":"10.1080/10826068.2025.2528396","DOIUrl":"https://doi.org/10.1080/10826068.2025.2528396","url":null,"abstract":"Nattokinase, an alkaline serine protease produced by Bacillus subtilis natto during natto fermentation, exhibits potent thrombolytic activity and is considered a promising next generation thrombolytic agent. Currently, most commercially available nattokinase is formulated as oral health supplements. However, unencapsulated nattokinase is rapidly inactivated due to the highly acidic gastric environment pH of approximately 1.2. Therefore, developing formulations that enhance enzymatic stability and bioavailability is crucial for its successful industrialization. In this study, we developed nattokinase microcapsules using a novel ternary composite embedding system composed of sodium alginate, gelatin, and chitosan. The optimal formulation prepared with a sodium alginate-gelatin mass ratio of 3:1, a total polymer concentration of 3%, and a chitosan concentration of 0.8%, achieved an enzyme activity recovery rate of 96.54%. Release studies in simulated gastrointestinal fluids demonstrated excellent gastric stability, with only 4.47% of nattokinase released over 2.5 h, which was 5-35% lower than that of single or binary encapsulation systems, significantly minimizing acid-induced inactivation. In contrast, sustained intestinal release was observed, reaching 91.58% at 48 h. This biphasic release profile ensures targeted intestinal delivery, where nattokinase can exert its therapeutic effects, while enabling acid resistance and controlled release.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recovery of polyphenolic compounds from Brassica oleracea leaves biowaste by ultrasound-assisted and maceration extraction: study of variables effect and kinetics using Peleg model. 超声辅助浸渍法回收甘蓝叶生物废弃物中多酚类化合物：Peleg模型变量效应及动力学研究

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-09 DOI: 10.1080/10826068.2025.2528398

Daniela Cerro, Catalina González, Cristian Armijo, Luisa Sepúlveda

{"title":"Recovery of polyphenolic compounds from Brassica oleracea leaves biowaste by ultrasound-assisted and maceration extraction: study of variables effect and kinetics using Peleg model.","authors":"Daniela Cerro, Catalina González, Cristian Armijo, Luisa Sepúlveda","doi":"10.1080/10826068.2025.2528398","DOIUrl":"https://doi.org/10.1080/10826068.2025.2528398","url":null,"abstract":"This study compared ultrasound-assisted extraction (UAE) and conventional extraction by maceration (CE) to obtain polyphenolic compounds with antioxidant activity from Brassica oleracea leaves biowaste. Preliminary tests showed that agitation intensity positively affected both the initial recovery rate and the equilibrium concentration of polyphenols with antioxidant activity. The equilibrium extraction time for CE was 150 minutes, while UAE achieved equilibrium in just 90 minutes. UAE also increased the initial recovery rate by more than seven times and improved the total recovery by 12%. Box-Behnken design was used to optimize CE, while factorial design was used for UAE, considering solid/liquid ratio (10-70 g/L), solvent concentration (20-90% v/v) and temperature (20 °C-60 °C). For CE, the highest results were obtained at 60 °C, 70% ethanol/water v/v and 10 g/L ratio, obtaining 1454.7 mg GAE/100 g DW, with 677.4 mg Fe+2/100 g DW of antioxidant capacity and 20.7 mg CA/100 g DW of chlorogenic acid. In comparison, the UAE method under similar conditions and in a lower time, achieved up to 1984.8 mg GAE/100 g DW, 913.4 mg Fe+2/100 g DW, and 39.7 mg CA/100 g DW. In conclusion, UAE is more efficient than CE for extracting polyphenolic compounds with antioxidant activity, allowing higher extraction yields.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted extraction of antioxidative peptides from tomato (Solanum lycopersicum) and their anti-angiogenic potential via molecular docking. 超声辅助提取番茄抗氧化肽及其分子对接抗血管生成潜能研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-09 DOI: 10.1080/10826068.2025.2528399

Poh Yee Lee, Hock Ing Chiu, Mohd Nazri Ismail

{"title":"Ultrasound-assisted extraction of antioxidative peptides from tomato (Solanum lycopersicum) and their anti-angiogenic potential via molecular docking.","authors":"Poh Yee Lee, Hock Ing Chiu, Mohd Nazri Ismail","doi":"10.1080/10826068.2025.2528399","DOIUrl":"https://doi.org/10.1080/10826068.2025.2528399","url":null,"abstract":"Bioactive peptides derived from natural sources have attracted significant attention for their antioxidative and anticancer potential. This study explores the potential of antioxidative peptides from Solanum lycopersicum var. Win Win, a new Malaysian tomato hybrid and their correlation with anticancer activity. Peptides were extracted using ultrasound-assisted extraction under optimized conditions: 4 mins extraction time, 57% amplitude, and 60% ethanol concentration, yielding 4.50 ± 0.11 mg/mL. The antioxidant activity of the peptide extracts was validated using DPPH and FRAP assays. Peptide identification through LC-MS/MS and PEAKS revealed 570 peptides. Identified peptides underwent in silico screening to refine lists for anticancer and antioxidant potential. This process also evaluated peptides that are lacking hemolytic, allergenic, and cytotoxic properties. Top five antioxidative peptides underwent molecular docking to evaluate the interaction with VEGFR2, a key target in cancer cells proliferation. Among them, YGYMC and RWYHNQ demonstrated better docking orientations with VEGFR2, suggesting strong binding affinity. These findings highlight the potential of tomato-derived peptides in cancer therapy development. Further in vitro validation is recommended to confirm their clinical applicability and efficacy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthocyanins from black glutinous rice bran: process efficiency and characterization of anthocyanins. 黑糯米糠花青素：工艺效率及花青素的表征。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-05 DOI: 10.1080/10826068.2025.2525213

Pan Wu, Fang Yin, Xiaojun He, Shen Yang, Hongxiang Zhou

{"title":"Anthocyanins from black glutinous rice bran: process efficiency and characterization of anthocyanins.","authors":"Pan Wu, Fang Yin, Xiaojun He, Shen Yang, Hongxiang Zhou","doi":"10.1080/10826068.2025.2525213","DOIUrl":"https://doi.org/10.1080/10826068.2025.2525213","url":null,"abstract":"Black glutinous rice bran (BGRB) serves as a plentiful natural pigment source. However, limited research has been conducted on the extraction, purification, and characterization of the anthocyanins present in BGRB. This work aims to provide an eco-friendly process for industrial anthocyanin extraction and purification. Following green chemistry principles, safe solvents and recyclable purification materials were used in the experiment. And waste production and energy consumption were limited. Optimal extraction conditions: ethanol-water ratio 40:60 (v/v, pH 2.5), solvent-feed ratio 80:1 (mL/g), 50 °C, and 40 min yielded 19.79 ± 0.14 mg/g anthocyanin, and 32 anthocyanins were identified in the BGRB anthocyanin extract. Optimal purification conditions: employing a D101/AB-8 mixed resin (7:3 w/w), the volume, pH value, and flow rate of BGRB anthocyanin extract were 1.5 Bed Volumes (BV), pH 3.0, and 0.5 BV/hr, respectively; desorption was performed successively with 40:60 (v/v, pH 2.5) ethanol-water of 6 BV, 0.5 BV/hr. Purification achieved 65.97% anthocyanin recovery. After purification, total anthocyanins (67.70 ± 5.43 mg/g to 171.27 ± 7.99 mg/g) and color value (33.48 ± 2.91 to 84.58 ± 1.41) were both 2.5 times higher than in the extract. The metabolite expression of purified anthocyanins showed 4.2 times increase by liquid chromatography-mass spectrometry (LC-MS).","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and characterization of terpolymer P(3HB-co-3HV-co-4HB) produced by Cupriavidus malaysiensis USMAA1020 using glycerol pitch. 以甘油酯为原料合成马来铜枝USMAA1020共聚物P（3HB-co-3HV-co-4HB）的生物合成及表征

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-07-05 DOI: 10.1080/10826068.2025.2522473

Musa Ibn Abbas, Nur Hanani Arif Shah, Amirul Al Ashraf Abdullah, Siti Nor Syairah Anis

{"title":"Biosynthesis and characterization of terpolymer P(3HB-co-3HV-co-4HB) produced by Cupriavidus malaysiensis USMAA1020 using glycerol pitch.","authors":"Musa Ibn Abbas, Nur Hanani Arif Shah, Amirul Al Ashraf Abdullah, Siti Nor Syairah Anis","doi":"10.1080/10826068.2025.2522473","DOIUrl":"10.1080/10826068.2025.2522473","url":null,"abstract":"This study demonstrates a circular economy approach by utilizing glycerol pitch, biodiesel co-product as a carbon source to produce biodegradable terpolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) [P(3HB-co-3HV-co-4HB)] using Cupriavidus malaysiensis USMAA1020. Various carbon sources, including oleic acid, 1,4-butanediol, and 1-pentanol, were combined with glycerol pitch as main substrates. The experiments were conducted in a mineral salt medium (MSM) with a high concentration of carbon source and limited nitrogen, which promotes both bacterial growth and PHA accumulation. Preliminary studies indicated, the composition of the polymer was triggered by varying the concentration of 1,4-butanediol, oleic acid, and glycerol pitch while keeping the amount of 1-pentanol constant. After optimization, the PHA content in dry biomass increased from 60 ± 12 to 88 ± 5 wt%, while the residual dry cell weight (RDCW) slightly decreased from 1.77 ± 0.53 to 1.11 ± 0.41 g/L. The combination of glycerol pitch in the biosynthesis of the terpolymer proved effective for the cost-efficient and sustainable production of P(3HB-co-3HV-co-4HB). This process could drive innovation while reducing waste, by harnessing glycerol pitch's properties, sustainable co-product of biodiesel production into a valuable biodegradable polymer. The characterization of the terpolymer revealed a diverse range of properties, making it suitable for various applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0