Preparative Biochemistry & Biotechnology最新文献

{"title":"Development of an efficient extraction and enrichment method for total flavonoids compounds from Erigeron breviscapus using ultrasound-assisted extraction and macroporous resin adsorption.","authors":"Yang Li, Wei Zhao, Qifeng Li, Jiayu Zhang, Anquan Yang, Min Xie, Ranhua Xiong, Chaobo Huang","doi":"10.1080/10826068.2025.2502757","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502757","url":null,"abstract":"<p><p>Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins' adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 °C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of sustainable bioprocessing for the production of high-value biomolecules (chlorophylls and proteins) using <i>Desmodesmus subspicatus</i>.","authors":"Sreya Sarkar, Sambit Sarkar, Sunil K Maity, Tridib Kumar Bhowmick, Kalyan Gayen","doi":"10.1080/10826068.2025.2502765","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502765","url":null,"abstract":"<p><p>Traditional protein and pigment (e.g., chlorophyll) sources are becoming insufficient due to the rapid rise of the global population in modern civilization. Microalgae offer a promising solution for protein and chlorophyll sources due to their higher productivity than terrestrial plants. This study aims to optimize the cultivation conditions for <i>Desmodesmus subspicatus</i>, a microalgal strain containing ∼60% protein and 4% chlorophyll, to enhance biomass, protein and chlorophyll productivity. A Taguchi Orthogonal Array (TOA) was used for systematic optimization of BG-11 medium components. Further experiments assessed the effects of light intensity and different carbon and nitrogen sources. Under optimized BG-11 conditions, biomass increased 1.3-fold, with protein and chlorophyll productivity rising 2.25 and 1.92-fold, respectively. Supplementation with carbon and nitrogen sources under varying light (84-504 µmol m^-2 s^-1) further enhanced yields by 1.6-fold. Glycine proved to be the most effective nitrogen source, while cellulose as a carbon source resulted in 2.4-fold higher biomass, 7.3-fold higher protein, and 2.3-fold higher chlorophyll. Cytotoxicity assessment of the extracted chlorophyll revealed over 94% A549 cell viability at concentrations up to 100 µg/mL, confirming its biocompatibility. Therefore, <i>Desmodesmus subspicatus</i> has promise as a sustainable source of proteins and chlorophylls in the nutraceutical and food industries.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-16"},"PeriodicalIF":2.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Weissella confusa</i> XG-3 EPS: production intensification by co-culturing with <i>Candida shehatae</i> and partial characterization.","authors":"Shuhan Yang, Shouqi Zhao, Yingying Ning, Dan Zhao","doi":"10.1080/10826068.2025.2504546","DOIUrl":"https://doi.org/10.1080/10826068.2025.2504546","url":null,"abstract":"<p><p>Previous studies have shown that co-culture methods can enhance exopolysaccharide production, no studies have reported this enhancement in co-cultures between <i>Weissella confusa</i> and <i>Candida shehatae</i>. This study developed an efficient co-culture system combining <i>W. confusa</i> XG-3 and <i>C. shehatae</i> (1:1 ratio), and employed response surface methodology to optimize both exopolysaccharide yield and properties. The initial co-culture produced 56.82 ± 0.12 g/L exopolysaccharide, a 1.63-fold increase over single culture. Optimization (sucrose: 105.97 g/L; yeast extract: 5.81 g/L; peptone: 6.24 g/L) further increased the yield to 115.66 g/L, a 2.04-fold improvement. The co-cultured exopolysaccharide had high water solubility (60.45 ± 0.70%) and water holding capacity (614.74 ± 9.26%). The co-cultured EPS showed significant antioxidant activity. At 5 mg/mL, the scavenging rates of ABTS, DPPH, hydroxyl, superoxide, H₂O₂ and total reducing power reached the highest 52.22 ± 0.47%, 50.79 ± 2.86%, 55.80 ± 2.56%, 36.05 ± 2.07%, 66.85 ± 0.30%, and 0.39 ± 0.01. It also had a superior effect on the proliferation of several probiotics compared with commercial prebiotics and exhibited high thermostability with a degradation threshold at 292.5 °C. These results demonstrate a novel, scalable strategy for producing multifunctional EPS with broad applications in the food industry.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of penicillinase on chitosan-modified gold electrodes for enhanced stability and potential biosensing applications.","authors":"Parneet Sheoran, Geetanjali Saini, Madhu Jangra, Anjum Gahlaut, Vikas Raj","doi":"10.1080/10826068.2025.2502771","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502771","url":null,"abstract":"<p><p>In this research, penicillinase was isolated from <i>Bacillus licheniformis</i> by ammonium sulfate precipitation, dialysis, sephadex-25 chromatography and sodium dodecyl sulfate (SDS)-PAGE. The enzyme was then attached to a chitosan- modified gold (Au) electrode surface via covalent bonds using GA as the linking agent. The immobilized enzyme's characteristics were evaluated by determining various parameters including pH and temperature optima, enzyme activity retention, and reusability potential. The substrate Penicillin G was employed for these assessments. Post-immobilization analysis showed that while the optimal pH range remained constant at 6.5-7.5, the temperature for maximum enzyme activity increased from 34 °C to 38 °C compared to the enzyme in solution. It was found that the immobilized enzyme maintained around 80% of its initial activity after being kept at 4 °C for a period of 30 days. When compared to the enzyme in its free state, the immobilization method made it more stable and usable. Even after 14 consecutive reaction cycles, the immobilized enzyme retained 38% of its initial catalytic activity.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of <i>Ganoderma lingzhi</i> triterpene extraction method and its hypoglycemic activity.","authors":"Shuang Hua, Yanshuang Li, Feng Jin, Meiyao Gan, Xianshun Jiang, Ying Zhang, Bo Zhang, Xiao Li","doi":"10.1080/10826068.2025.2490973","DOIUrl":"https://doi.org/10.1080/10826068.2025.2490973","url":null,"abstract":"<p><p>To optimize the continuous reflux extraction process of triterpenes from <i>Ganoderma lingzhi</i> using an orthogonal test and to lay a foundation for its industrial application, an orthogonal experiment was carried out for optimization. The optimal conditions were obtained and verified. The continuous reflux extraction technology for triterpenes from <i>G. lingzhi</i> was optimized. The vanillin-glacial acetic acid method was used to determine the content of triterpenes in <i>G. lingzhi</i>. The optimal process parameters were as follows: an ethanol concentration of 80%, an extraction time of 1.5 hr, and a solid-liquid ratio of 1:26. The average extraction yield was 2.412%, with a relative standard deviation of 1.079%. Taking the inhibition rate of α-glucosidase activity as the index of evaluating hypoglycemic activity, <i>G. lingzhi</i> triterpene has a significant effect on hypoglycemic activity. The optimized triterpene reflux extraction process for <i>G. lingzhi</i> is feasible and stable, providing a scientific reference for the extraction of effective components from <i>G. lingzhi</i> in subsequent stages. The red text indicates deleted content, and the yellow text indicates added content. In order to optimize the continuous reflux extraction process of triterpenes from <i>G. lingzhi</i> through orthogonal testing and to establish a foundation for its industrial application, an orthogonal experiment was conducted for optimization purposes. The optimal conditions were determined and validated. Subsequently, the continuous reflux extraction technique for triterpenes from <i>G. lingzhi</i> was optimized. The vanillin-glacial acetic acid method determined triterpene content. Optimal parameters were: ethanol concentration of 80%, extraction time of 1.5 h, and solid-liquid ratio of 1:26, with an average extraction yield of 2.412% (RSD = 1.079%). Using α-glucosidase activity inhibition rate as an index, <i>G. lingzhi</i> triterpenes showed significant hypoglycemic activity. The optimized extraction process is feasible and stable, offering a scientific reference for subsequent extraction of <i>G. lingzhi</i>'s effective components.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization, optimization, characterization and kinetic properties of polyphenol oxidase to multi-walled carbon nanotube.","authors":"Şeyma Çam, Mehmet Doğan, Pınar Turan Beyli, Serap Doğan, Zeynep Bicil, Berna Koçer Kızılduman","doi":"10.1080/10826068.2025.2498460","DOIUrl":"https://doi.org/10.1080/10826068.2025.2498460","url":null,"abstract":"<p><p>In this study, the kinetic properties of polyphenol oxidase (PPO) extracted from <i>Satureja cuneifolia</i> were investigated using catechol and 4-methylcatechol as substrates. Optimal pH and temperature values were determined at each purification step. Subsequently, the optimum immobilization conditions were established as 2 hours of stirring time and 0.05 g of multi-walled carbon nanotubes (MWCNTs). Characterization by BET, FTIR, DTA/TG, TEM, and SEM/EDX analyses confirmed the successful immobilization of PPO onto mesoporous MWCNTs, with notable changes in surface morphology and thermal degradation behavior. The optimum pH for the free enzyme remained constant across purification methods but varied with the substrate, while the optimum temperature was consistently found at 30 °C. Upon immobilization, the optimum temperature shifted to higher values, indicating enhanced thermal stability. Catalytic efficiency (Vmax/K_M) for catechol decreased significantly after immobilization (from 2.5 × 10⁶ to 5 × 10⁴ min^-1), whereas for 4-methylcatechol, the immobilized enzyme retained a high catalytic efficiency (Vmax/K_M =1 × 10⁶ min^-1), comparable to that of the free enzyme. This shift suggests that immobilization favored substrate specificity toward 4-methylcatechol. Overall, the MWCNT-PPO system demonstrated enhanced stability, improved reusability, and altered substrate selectivity, making it a strong candidate for industrial biocatalytic applications where operational durability and efficiency are critical.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic hydrolysis of lignocellulosic biomass using co-immobilization of tri-enzymes on chitosan-magnetite nanoparticle beads.","authors":"Sushil Nagar, Meena Sindhu, Kajal Kumari, Vinay Kumar, Gulab Singh, Neeraj Kharor, Vishal Chugh, Vinod Kumar","doi":"10.1080/10826068.2025.2496255","DOIUrl":"https://doi.org/10.1080/10826068.2025.2496255","url":null,"abstract":"<p><p>This paper presents the co-immobilization of three enzymes-laccase, cellulase, and xylanase-on chitosan-magnetite nanoparticle beads, with process parameters optimized using response surface methodology on glutaraldehyde-activated chitosan-magnetite beads. The optimization achieved an impressive immobilization yield of 95.25%. Following immobilization on chitosan-magnetite beads (CMBs), the kinetic properties (Km and Vmax), as well as the optimal pH and temperature, were significantly enhanced. The immobilized LCX demonstrated excellent reusability, maintaining 51% of its initial activity after five consecutive cycles, and could be easily recovered using an external magnet. Maximum digestibility of cellulose (% Dc), hemicellulose (% DH), and lignin (% DL) was observed when 10 g of pretreated wheat bran was treated with 20 LCX-loaded CMBs at 40 °C for 60 minutes. The digestibility values for cellulose, hemicellulose, and lignin were 42.10 ± 1.85%, 52.30 ± 2.05%, and 18.12 ± 0.96%, respectively, using immobilized LCX-CMBs-1.0 to 1.5 times higher than those obtained with free enzymes. Additionally, the yield of reducing sugars was 62.17% for immobilized LCX compared to 46.06% for free LCX. The immobilization on CMBs offers an easily removable and cost-effective solution for various industrial applications.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of GABA producing strains from sourdoughs and optimization of GABA production by response surface-integrated particle swarm approach.","authors":"Zuhal Alkay, Abdullah Tahir Şensoy, Muhammed Zeki Durak, Yunus Emre Tuncil, Enes Dertli","doi":"10.1080/10826068.2025.2498456","DOIUrl":"https://doi.org/10.1080/10826068.2025.2498456","url":null,"abstract":"<p><p>Lactic Acid Bacteria (LAB) strains were isolated from sourdough samples collected from five provinces of Türkiye and the Cyprus region. The gamma-aminobutyric acid (GABA) production ability of these LAB strains with (+) gad gene regions was evaluated. Response Surface Method (RSM) and Particle Swarm Optimization (PSO) techniques were used together to optimize the GABA environment of <i>Levilactobacillus brevis</i> SD48 strain with the highest GABA production. Optimization of fermentation conditions for GABA production of <i>Levilactobacillus brevis</i> SD48 strain was designed with Box-Behnken design. Optimization parameters tested were pH, temperature, time and monosodium glutamate (MSG) concentration. Fermentation time (24, 48, 72 hours), pH values (4.5, 5.75 and 7.0), temperature (30, 35 and 40 °C), MSG concentration (50, 225, 400 mM) were taken. It was seen that the best process parameters that allow maximum GABA yield should be temperature 30 °C, MSG concentration 130.71 mM, pH 4.74 and time 72 hour. According to the results, it can be thought that microbial production of GABA is both cheap and the optimized conditions we determined will play an important role in the production of GABA-rich fermented foods.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system.","authors":"Xiaotong Zheng, Ting Zheng, Wen Wang, Xuejun Cao, Junfen Wan","doi":"10.1080/10826068.2025.2478402","DOIUrl":"https://doi.org/10.1080/10826068.2025.2478402","url":null,"abstract":"<p><p>Spiramycin is a 16-membered macrolide antibiotic widely used in the medical field. Industrial extraction of antibiotics from fermentation broth using organic solvents raises various environmental and health concerns. In this study, a thermosensitive polymer, NPE-108, was used to construct an aqueous two-phase system (ATPS) for the extraction of spiramycin. We used Gromacs software to develop a molecular dynamics simulation model to reveal the distribution mechanism of spiramycin molecules in the NPE-108/water ATPS from a microscopic perspective. Additionally, we examined the effects of volume ratio, temperature, and pH on extraction through experimentation. Under the optimal conditions for forward extraction, the distribution coefficient and extraction efficiency were 25.3 and 89.9%, respectively. Under the optimal conditions for back extraction, the distribution coefficient and extraction efficiency were 6.8 and 81.5%, respectively. Optimization of crystallization conditions resulted in a crystal yield of 88.1% and a purity of 98.4%. The content of both spiramycin components and impurities in the crystalline sample met the requirements of the European Pharmacopeia. The results of this study provided insights into molecular interactions and the extraction process, offering a more environmentally friendly and economically viable alternative for industrial spiramycin production.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":2.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of medical-grade biopolymer in air lift bioreactors.","authors":"Navodit Kumar Singh, Ashok Kumar Srivastava, T R Sreekrishnan, Srividya Shivakumar","doi":"10.1080/10826068.2025.2496246","DOIUrl":"https://doi.org/10.1080/10826068.2025.2496246","url":null,"abstract":"<p><p>Microbes are known to produce biopolymers for societal applications. Economical production of biopolymer (PHB) is desperately required to significantly replace or reduce usage of non-degradable polypropylene produced by disappearing petroleum resources. Besides it is also equally important to ensure abundant availability of low cost medical grade biopolymers which can be used for several medical applications in society. It has been invariably observed that mechanical agitation in the bioreactors features major power consumption in the operation of bioreactors therefore usage of air lift bioreactors are likely to reduce power consumption by mechanical agitation significantly thereby leading to economic biopolymer production. Present investigation evaluates the possible role of pneumatic bioreactors (e.g., Bubble Column, Outer Aeration Inner Settling, Inner Aeration Outer Settling) as alternates to mechanically agitated bioreactors for the economic production of medical grade biopolymers P(3HB) by <i>Bacillus thuringiensis</i> IAM12077 using glycerol and glucose as major substrates. It was observed that <i>Bacillus thuringiensis</i> IAM12077 cultivations featured Biopolymer P(3HB) accumulations of 22.48%, 37.07%, 27.73%, in BC, OAIS, IAOS air lift bioreactors. Relatively higher product yield, volumetric productivity and P(3HB) accumulation was observed in Outer Aeration Inner Settling (OAIS) air lift bioreactor configuration as opposed to other pneumatic bioreactors.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}