Preparative Biochemistry & Biotechnology最新文献

Isolation and identification of a salt-tolerant Coelastrum sp. and exploration of its potential for biodiesel production. 分离和鉴定一种耐盐鹅掌霉菌并探索其生产生物柴油的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-24 DOI: 10.1080/10826068.2024.2405941

Jing Xu, Han Wang, Jixin Liu, Jingping Ge, Yimeng Lin, Wenxiang Ping

引用次数: 0

Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment. 利用实验统计设计优化新型肠球菌的外多糖生产。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-20 DOI: 10.1080/10826068.2024.2402337

Shivani Singh Gaur, Uday S Annapure

{"title":"Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment.","authors":"Shivani Singh Gaur, Uday S Annapure","doi":"10.1080/10826068.2024.2402337","DOIUrl":"https://doi.org/10.1080/10826068.2024.2402337","url":null,"abstract":"The Exopolysaccharide (EPS) producing novel strains of Enterococcus previously isolated from the vaginal source of pregnant women were selected based on ropy structure formation. The two selected strains, E.villorum SB-2 and E.rivorum S22-3, were found to be producing 2.87 g/l and 3.14 g/l EPS, respectively, in the minimal media (M17 media) after 24-hour fermentation under anaerobic condition. Both the strains have probiotic properties and have the potential to be used for industrial applications. The production media and fermentation conditions were optimized to enhance the EPS production using the one-factor method, Placket-Burman factorial designing and Central composite design (CCD) of Response surface methodology (RSM). The most relevant factors affecting the EPS yield were sucrose, yeast extract and pH for E.villorum SB2 and sucrose, yeast extract and magnesium sulfate for the E.rivorum S22-3 as determined by Placket-Burman design, whose concentrations were further optimized using CCD. The optimized fermentation conditions gave the total EPS of 9.76 g/l (4 times the initial production) from E.villorum SB-2 and 7.74 g/l (2.5 times the initial production) from E.rivorum S22-3, respectively, after 36-hour incubation at 37 °C. These optimization studies might be helpful during scale-up process for the industrial scale production of these exopolysaccharide.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing methane yield and shifting microbial communities in anaerobic reactors treating lipid-rich dairy wastewater through exogenous lipase addition 通过添加外源脂肪酶提高处理富含脂质的乳制品废水的厌氧反应器中的甲烷产量并改变微生物群落

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10826068.2024.2399042

Marwa Abedalkarem, Omamah Dabbour, Sare Asli

引用次数: 0

Disposable glutamate biosensor based on platinum nanoparticles, carbon quantum dots and poly-L-aspartic acid. 基于铂纳米粒子、碳量子点和聚天冬氨酸的一次性谷氨酸生物传感器。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10826068.2024.2402340

Ceren Kaçar Selvi,Barış Şenceol,Pınar Esra Erden

{"title":"Disposable glutamate biosensor based on platinum nanoparticles, carbon quantum dots and poly-L-aspartic acid.","authors":"Ceren Kaçar Selvi,Barış Şenceol,Pınar Esra Erden","doi":"10.1080/10826068.2024.2402340","DOIUrl":"https://doi.org/10.1080/10826068.2024.2402340","url":null,"abstract":"This study reports the design and development of a disposable amperometric biosensor for the determination of L-glutamate. Glutamate oxidase (GlOx) was immobilized onto a screen-printed carbon electrode (SPE) modified with poly-L-Aspartic acid (PAsp), carbon quantum dots (CQD), and platinum nanoparticles (PtNP) for the construction of the biosensor. The surface composition of the modified SPE was optimized using the one variable at a time method. The morphological properties of the biosensor were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The electrochemical behavior of the modified electrodes was studied by cyclic voltammetry. Under the optimized experimental conditions the linear working range, detection limit and sensitivity of the GlOx/PtNP/CQD/PAsp/SPE were found to be 1.0 - 140 µM, 0.3 µM and 0.002 µA µM-1, respectively. The GlOx/PtNP/CQD/PAsp/SPE biosensor also exhibited good measurement repeatability. The as-developed biosensor was applied for the determination of L-glutamate in spiked serum samples and the average analytical recovery of added glutamate was 98.9 ± 3.9%.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142178366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2,3-Butanediol plus acetoin obtention by Enterobacter aerogenes ATCC 13048: inhibition by target products and cells reuse during fed-batch cultivation 产气肠杆菌 ATCC 13048 获取 2,3-丁二醇和乙炔酮：目标产品的抑制作用和喂料批次培养过程中细胞的再利用

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10826068.2024.2402341

Bruna Campos de Souza, Analia Borges Folle, Sabrina Carra, Eloane Malvessi

引用次数: 0

Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate. 以麦麸为低成本底物，通过固态发酵法利用真菌 Pleurotus djamor 生产凝乳蛋白酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-02 DOI: 10.1080/10826068.2024.2399040

Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira

{"title":"Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate.","authors":"Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira","doi":"10.1080/10826068.2024.2399040","DOIUrl":"https://doi.org/10.1080/10826068.2024.2399040","url":null,"abstract":"Proteases are enzymes that hydrolyze peptide bonds present in proteins and peptides. They are widely used for various industrial applications, such as in the detergent, food, and dairy industries. Cheese is one of the most important products of the dairy industry, and the coagulation stage is crucial during the cheese-making process. Enzymatic coagulation is the most common technique utilized for this purpose. Microbial enzymes are frequently used for coagulation due to their advantages in terms of availability, sustainability, quality control, product variety, and compliance with dietary and cultural/religious requirements. In the present study, we identified and subsequently characterized milk coagulant activity from the fungus Pleurotus djamor PLO13, obtained during a solid-state fermentation process, using the agro-industrial residue, wheat bran, as the fermentation medium. Maximum enzyme production and caseinolytic activity occurred 120 h after cultivation. When the enzyme activity against various protease-specific synthetic substrates and inhibitors was analyzed, the enzyme was found to be a serine protease, similar to elastase 2. This elastase-2-like serine protease was able to coagulate pasteurized whole and reconstituted skim milk highly efficiently in the presence and absence of calcium, even at room temperature. The coagulation process was influenced by factors such as temperature, time, and calcium concentration. We demonstrate here, for the first time, an elastase-2-like enzyme in a microorganism and its potential application in the food industry for cheese production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of monoterpenes using Streptomyces strains from the rhizosphere of Inga edulis Martius from in an Amazonian urban forest fragment. 利用来自亚马逊城市森林片区 Inga edulis Martius 根圈的链霉菌株对单萜烯进行生物转化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-12 DOI: 10.1080/10826068.2024.2315476

Elison de Souza Sevalho, Rafael de Souza Rodrigues, Antonia Queiroz Lima de Souza, Afonso Duarte Leão de Souza

{"title":"Biotransformation of monoterpenes using Streptomyces strains from the rhizosphere of Inga edulis Martius from in an Amazonian urban forest fragment.","authors":"Elison de Souza Sevalho, Rafael de Souza Rodrigues, Antonia Queiroz Lima de Souza, Afonso Duarte Leão de Souza","doi":"10.1080/10826068.2024.2315476","DOIUrl":"10.1080/10826068.2024.2315476","url":null,"abstract":"To investigate the biocatalytic potential of Amazonian actinomycetes for monoterpenes biotransformation. To carry out the present study, eleven actinomycetes of the genus Streptomyces isolated from inga-cipó (Inga edulis Mart.) rhizospheres were tested for their ability to bioconvert the substrates R-(+)-limonene, S-(-)-limonene, 1S-(-)-α-pinene, and (-)-β-pinene as sole carbon and energy source. According to gas chromatography-mass spectrometry analysis, three strains, LabMicra B270, LaBMicrA B310, and LaBMicrA B314, were able to biotransform 1S-(-)-α-pinene after 96 h of growth. However, Streptomyces LaBMicrA B270 was the most promising since it converted after only 72 h all the 1S-(-)-α-pinene mainly into cis-verbenol (74.9±1.24%) and verbenone (18.2±1.20%), compounds that have important biological activities and great industrial interest as additives in foods and cosmetics. These findings can stimulate the development of natural aromas using naturally abundant monoterpenes, ratify the potential of microorganisms from almost unexplored niches such as the Amazonian rhizosphere, and reinforce the importance of preserving those niches.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of various light spectra on amino acids and pigment production of Arthrospira platensis using flat-plate photobioreactor. 利用平板式光生物反应器研究不同光谱对板浆节肢动物氨基酸和色素产量的影响

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2021-07-21 DOI: 10.1080/10826068.2021.1941102

Hanieh Tayebati, Farshid Pajoum Shariati, Neda Soltani, Hessam Sepasi Tehrani

{"title":"Effect of various light spectra on amino acids and pigment production of Arthrospira platensis using flat-plate photobioreactor.","authors":"Hanieh Tayebati, Farshid Pajoum Shariati, Neda Soltani, Hessam Sepasi Tehrani","doi":"10.1080/10826068.2021.1941102","DOIUrl":"10.1080/10826068.2021.1941102","url":null,"abstract":"Today, the use of nutrients derived from natural bioactive compounds application in the food, pharmaceutical, and cosmetic industries is on the increase. This paper aimed to evaluate the amino acids profile (essential and non-essential) and pigments composition (chlorophyll a, carotenoids, and phycocyanin) of Arthrospira platensis (a blue-green microalga) cultivation in a flat-plate photobioreactor under various types of light-emitting diodes (red: 620-680 nm, white: 380-780 nm, yellow: 570-600nm, blue: 445-480 nm). The maximum biomass concentration (604.96 mg L-1) occurred when the red LED was applied for cultivation, and the minimum biomass concentration (279.39 mg L-1) was obtained under blue LED. The sequence of pigments and amino acids concentrations (mg L-1culture volume) was approximately in accordance with the biomass productivity. It means the red light produces the maximum concentration of pigments (chlorophyll a: 5.42, carotenoids: 2.92, phycocyanin: 67.54 mg L-1) and amino acids (essential amino acids: 110.47, nonessential amino acids: 179.10 mg L-1). Nevertheless, when these values were measured in mg per g of dry weight, the utmost contents were observed in microalgal products cultivated under blue LED. These consequences are due to the highest cell productivity and the most extended length of cells that occurred under red and blue LEDs, respectively.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39210075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-12 DOI: 10.1080/10826068.2024.2325245

引用次数: 0

Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system. 在大肠杆菌表达系统中生产来自莫隆尼鼠白血病病毒的逆转录酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-02-27 DOI: 10.1080/10826068.2024.2317311

Yudhi Nugraha, Fina Amreta Laksmi, Isa Nuryana, Helbert, Firyal Nida Khasna

{"title":"Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system.","authors":"Yudhi Nugraha, Fina Amreta Laksmi, Isa Nuryana, Helbert, Firyal Nida Khasna","doi":"10.1080/10826068.2024.2317311","DOIUrl":"10.1080/10826068.2024.2317311","url":null,"abstract":"Reverse transcriptase (RT) is one of the most important enzymes used in molecular biology applications, enabling the conversion of RNA into complementary DNA (cDNA) that is used in reverse transcription-polymerase chain reaction (RT-PCR). The high demand of RT enzymes in biotechnological applications making the production optimization of RT is crucial for meeting the growing demand in industrial settings. Conventionally, the expression of recombinant RT is T7-induced promoter using IPTG in Escherichia coli expression systems, which is not cost-efficient. Here, we successfully made an alternative procedure for RT expression from Moloney murine leukemia virus (M-MLV) using autoinduction method in chemically defined medium. The optimization of carbon source composition (glucose, lactose, and glycerol) was analyzed using Response Surface Methodology (RSM). M-MLV RT was purified for further investigation on its activity. A total of 32.8 mg/L purified M-MLV RT was successfully obtained when glucose, glycerol, and lactose were present at concentration of 0.06%, 0.9%, and 0.5% respectively, making a 3.9-fold improvement in protein yield. In addition, the protein was produced in its active form by displaying 7462.50 U/mg of specific activity. This study provides the first step of small-scale procedures of M-MLV RT production that make it a cost-effective and industrially applicable strategy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0