Preparative Biochemistry & Biotechnology最新文献

{"title":"Preparation of 5-hydroxymethylfurfural from cellulose catalyzed by silicon-based composite solid acids.","authors":"Zhen Liu, Run-Jian Jia, Jing-Jing Liu","doi":"10.1080/10826068.2025.2509895","DOIUrl":"https://doi.org/10.1080/10826068.2025.2509895","url":null,"abstract":"<p><p>In order to improve the efficiency of solid acid catalyzed cellulose preparation of 5-hydroxymethylfurfural (HMF), this study prepared a silica-based solid acid with dual acid sites comprising both Lewis and Brønsted acids. Firstly, SiO₂ microspheres prepared by reverse microemulsion method were used as the carrier, and the surface was modified with -SO₃H and -NH₂, and finally Cr³⁺ was loaded on to SiO₂ to prepare the composite solid acid catalyst Cr-SiO₂-SO₃H, and its characterization and catalytic performance were determined. The experimental results showed that in the two-phase reaction system composed of [BMIM]Cl and THF, using microcrystalline cellulose (MCC) as the substrate, when the reaction temperature was 160 °C, the reaction time was 4 h, the addition amount of Cr-SiO₂-SO₃H was 1 g/g (cellulose), and the volume ratio of [BMIM]Cl to THF was 1:3, the maximum yield of HMF could reach 54.8%, which was 1.49 and 1.34 times the maximum yield of SiO₂-SO₃H and Cr-SiO₂, respectively.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-7"},"PeriodicalIF":2.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of new bio-activated carbon filled epoxy composites.","authors":"Hatice Karaer Yağmur, Kübra Baykara, Seyithan Sönmez","doi":"10.1080/10826068.2025.2509307","DOIUrl":"https://doi.org/10.1080/10826068.2025.2509307","url":null,"abstract":"<p><p>In this study, active carbons were obtained by carbonizing waste laurel fruit seeds at 500 °C for 1 hour. The surface area of the activated carbon obtained using the ZnCl₂ activator was determined as 873.93 m²/g while the surface area of the activated carbon obtained without using the activator was determined as 1.52 m²/g. Furthermore, in order to obtain epoxy composites with improved mechanical and thermal properties, activated carbons were added to epoxy as filler at rates of 0%, 5% and 10% and bio-based epoxy composites were obtained. Epoxy composites were produced using a mold prepared according to ASTM D638 TYPE I standard. Thermal and tensile properties of filled and unfilled epoxy composites were investigated. LOI values of NE, N5, N10, A5 and A10 composites were determined as 23.48, 24.28, 25.32, 26.55 and 27.06, respectively. Surface hardness and tensile strength of composites containing activated carbon were found to be higher than pure epoxy composite. The tensile test results obtained for NE, N5, N10, A5 and A10 were as follows: elongation percentages at break were 5.37%, 1.80%, 11.59%, 2.21% and 1.61% and tensile stress were 19.12, 11.52, 13.63, 16.45 and 20.33 N/mm².</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient whole-cell biosynthesis of S-adenosyl-L-methionine by the engineered <i>Escherichia coli</i> with high ATP regenerating system.","authors":"Zhang Pengfei, Siqi Zuo, Peijie Shen, Zhengjun Si, Peilian Wei, Zhinan Xu","doi":"10.1080/10826068.2025.2509892","DOIUrl":"https://doi.org/10.1080/10826068.2025.2509892","url":null,"abstract":"<p><p>S-adenosyl-L-methionine (SAM) is an important intermediate metabolite and widely used in the treatment of liver disease, arthritis, and depression. In this work, a whole-cell catalysis strategy was employed to enhance SAM production by combining the SAM biosynthetic pathway with an adenosine triphosphate (ATP) regeneration system in <i>Escherichia coli.</i> Specifically, the <i>ado1</i>, <i>ack</i>, and <i>adk</i> genes were previously introduced into the genome of the host strain. We then confirmed the availability of the ATP regeneration system under the condition of adding adenosine monophosphate (AMP) and acetyl phosphate (ACP) as supplements. To improve the SAM production, the <i>sam2</i> gene derived from <i>Saccharomyces cerevisiae</i> was overexpressed using the plasmid pGEX-2TK in the strain and the conditions of biocatalytic process were optimized. Under the optimal biocatalytic conditions, the recombinant strain RS01 (pGEX-2TK-SAM2) achieved a SAM titer of 11.4 g/L after 10 h cultivation. This work not only provides a new platform for the efficient production of SAM but also offers insights into the biosynthesis of other ATP-dependent products.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological advances in torularhodin production: artificial neural networks as a tool for improving and biocompatibility studies.","authors":"Júlio Gabriel Oliveira de Lima, Ariane Alves Oshiro, Felipe Falcão Haddad, André de Souza Alves Guimarães, Cauê Benito Scarim, Álvaro de Baptista Neto, Valéria C Santos-Ebinuma","doi":"10.1080/10826068.2025.2502767","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502767","url":null,"abstract":"<p><p>Torularhodin is a bioactive carotenoid synthesized by certain microorganisms through complex cellular processes regulated by factors like nutrient availability. However, enhancing torularhodin production is a challenging task that requires costly and time-intensive experimental approaches. To address these limitations, computational modeling and simulation have become valuable tools for predicting and optimizing carotenoid biosynthesis. Among these techniques, polynomial models derived from multiple regressions provide useful insights but often struggle with the nonlinear nature of biological systems. In contrast, Artificial Neural Networks (ANNs) offer a more flexible alternative, improving predictive accuracy where traditional models fall short. This study aimed to optimize torularhodin production in <i>Rhodotorula glutinis</i> using ANN-based simulations and Response Surface Methodology (RSM) while also assessing the biocompatibility of the crude extract containing carotenoids. An experimental design with two independent variables (Tween 80 and malt extract) was implemented to evaluate their impact on torularhodin yield. ANN modeling successfully increased torularhodin production by approximately 10.69%, demonstrating its efficiency in bioprocess optimization. Additionally, microbial biomass extracts containing carotenoids exhibited biocompatibility in the Chorioallantoic Membrane assay, suggesting potential applications in pharmaceutical and food industries. These findings reinforce the importance of ANN modeling in optimizing microbial carotenoid production for sustainable biotechnology.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an efficient extraction and enrichment method for total flavonoids compounds from Erigeron breviscapus using ultrasound-assisted extraction and macroporous resin adsorption.","authors":"Yang Li, Wei Zhao, Qifeng Li, Jiayu Zhang, Anquan Yang, Min Xie, Ranhua Xiong, Chaobo Huang","doi":"10.1080/10826068.2025.2502757","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502757","url":null,"abstract":"<p><p>Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins' adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 °C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of sustainable bioprocessing for the production of high-value biomolecules (chlorophylls and proteins) using <i>Desmodesmus subspicatus</i>.","authors":"Sreya Sarkar, Sambit Sarkar, Sunil K Maity, Tridib Kumar Bhowmick, Kalyan Gayen","doi":"10.1080/10826068.2025.2502765","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502765","url":null,"abstract":"<p><p>Traditional protein and pigment (e.g., chlorophyll) sources are becoming insufficient due to the rapid rise of the global population in modern civilization. Microalgae offer a promising solution for protein and chlorophyll sources due to their higher productivity than terrestrial plants. This study aims to optimize the cultivation conditions for <i>Desmodesmus subspicatus</i>, a microalgal strain containing ∼60% protein and 4% chlorophyll, to enhance biomass, protein and chlorophyll productivity. A Taguchi Orthogonal Array (TOA) was used for systematic optimization of BG-11 medium components. Further experiments assessed the effects of light intensity and different carbon and nitrogen sources. Under optimized BG-11 conditions, biomass increased 1.3-fold, with protein and chlorophyll productivity rising 2.25 and 1.92-fold, respectively. Supplementation with carbon and nitrogen sources under varying light (84-504 µmol m^-2 s^-1) further enhanced yields by 1.6-fold. Glycine proved to be the most effective nitrogen source, while cellulose as a carbon source resulted in 2.4-fold higher biomass, 7.3-fold higher protein, and 2.3-fold higher chlorophyll. Cytotoxicity assessment of the extracted chlorophyll revealed over 94% A549 cell viability at concentrations up to 100 µg/mL, confirming its biocompatibility. Therefore, <i>Desmodesmus subspicatus</i> has promise as a sustainable source of proteins and chlorophylls in the nutraceutical and food industries.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-16"},"PeriodicalIF":2.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Weissella confusa</i> XG-3 EPS: production intensification by co-culturing with <i>Candida shehatae</i> and partial characterization.","authors":"Shuhan Yang, Shouqi Zhao, Yingying Ning, Dan Zhao","doi":"10.1080/10826068.2025.2504546","DOIUrl":"https://doi.org/10.1080/10826068.2025.2504546","url":null,"abstract":"<p><p>Previous studies have shown that co-culture methods can enhance exopolysaccharide production, no studies have reported this enhancement in co-cultures between <i>Weissella confusa</i> and <i>Candida shehatae</i>. This study developed an efficient co-culture system combining <i>W. confusa</i> XG-3 and <i>C. shehatae</i> (1:1 ratio), and employed response surface methodology to optimize both exopolysaccharide yield and properties. The initial co-culture produced 56.82 ± 0.12 g/L exopolysaccharide, a 1.63-fold increase over single culture. Optimization (sucrose: 105.97 g/L; yeast extract: 5.81 g/L; peptone: 6.24 g/L) further increased the yield to 115.66 g/L, a 2.04-fold improvement. The co-cultured exopolysaccharide had high water solubility (60.45 ± 0.70%) and water holding capacity (614.74 ± 9.26%). The co-cultured EPS showed significant antioxidant activity. At 5 mg/mL, the scavenging rates of ABTS, DPPH, hydroxyl, superoxide, H₂O₂ and total reducing power reached the highest 52.22 ± 0.47%, 50.79 ± 2.86%, 55.80 ± 2.56%, 36.05 ± 2.07%, 66.85 ± 0.30%, and 0.39 ± 0.01. It also had a superior effect on the proliferation of several probiotics compared with commercial prebiotics and exhibited high thermostability with a degradation threshold at 292.5 °C. These results demonstrate a novel, scalable strategy for producing multifunctional EPS with broad applications in the food industry.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of penicillinase on chitosan-modified gold electrodes for enhanced stability and potential biosensing applications.","authors":"Parneet Sheoran, Geetanjali Saini, Madhu Jangra, Anjum Gahlaut, Vikas Raj","doi":"10.1080/10826068.2025.2502771","DOIUrl":"https://doi.org/10.1080/10826068.2025.2502771","url":null,"abstract":"<p><p>In this research, penicillinase was isolated from <i>Bacillus licheniformis</i> by ammonium sulfate precipitation, dialysis, sephadex-25 chromatography and sodium dodecyl sulfate (SDS)-PAGE. The enzyme was then attached to a chitosan- modified gold (Au) electrode surface via covalent bonds using GA as the linking agent. The immobilized enzyme's characteristics were evaluated by determining various parameters including pH and temperature optima, enzyme activity retention, and reusability potential. The substrate Penicillin G was employed for these assessments. Post-immobilization analysis showed that while the optimal pH range remained constant at 6.5-7.5, the temperature for maximum enzyme activity increased from 34 °C to 38 °C compared to the enzyme in solution. It was found that the immobilized enzyme maintained around 80% of its initial activity after being kept at 4 °C for a period of 30 days. When compared to the enzyme in its free state, the immobilization method made it more stable and usable. Even after 14 consecutive reaction cycles, the immobilized enzyme retained 38% of its initial catalytic activity.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of <i>Ganoderma lingzhi</i> triterpene extraction method and its hypoglycemic activity.","authors":"Shuang Hua, Yanshuang Li, Feng Jin, Meiyao Gan, Xianshun Jiang, Ying Zhang, Bo Zhang, Xiao Li","doi":"10.1080/10826068.2025.2490973","DOIUrl":"https://doi.org/10.1080/10826068.2025.2490973","url":null,"abstract":"<p><p>To optimize the continuous reflux extraction process of triterpenes from <i>Ganoderma lingzhi</i> using an orthogonal test and to lay a foundation for its industrial application, an orthogonal experiment was carried out for optimization. The optimal conditions were obtained and verified. The continuous reflux extraction technology for triterpenes from <i>G. lingzhi</i> was optimized. The vanillin-glacial acetic acid method was used to determine the content of triterpenes in <i>G. lingzhi</i>. The optimal process parameters were as follows: an ethanol concentration of 80%, an extraction time of 1.5 hr, and a solid-liquid ratio of 1:26. The average extraction yield was 2.412%, with a relative standard deviation of 1.079%. Taking the inhibition rate of α-glucosidase activity as the index of evaluating hypoglycemic activity, <i>G. lingzhi</i> triterpene has a significant effect on hypoglycemic activity. The optimized triterpene reflux extraction process for <i>G. lingzhi</i> is feasible and stable, providing a scientific reference for the extraction of effective components from <i>G. lingzhi</i> in subsequent stages. The red text indicates deleted content, and the yellow text indicates added content. In order to optimize the continuous reflux extraction process of triterpenes from <i>G. lingzhi</i> through orthogonal testing and to establish a foundation for its industrial application, an orthogonal experiment was conducted for optimization purposes. The optimal conditions were determined and validated. Subsequently, the continuous reflux extraction technique for triterpenes from <i>G. lingzhi</i> was optimized. The vanillin-glacial acetic acid method determined triterpene content. Optimal parameters were: ethanol concentration of 80%, extraction time of 1.5 h, and solid-liquid ratio of 1:26, with an average extraction yield of 2.412% (RSD = 1.079%). Using α-glucosidase activity inhibition rate as an index, <i>G. lingzhi</i> triterpenes showed significant hypoglycemic activity. The optimized extraction process is feasible and stable, offering a scientific reference for subsequent extraction of <i>G. lingzhi</i>'s effective components.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization, optimization, characterization and kinetic properties of polyphenol oxidase to multi-walled carbon nanotube.","authors":"Şeyma Çam, Mehmet Doğan, Pınar Turan Beyli, Serap Doğan, Zeynep Bicil, Berna Koçer Kızılduman","doi":"10.1080/10826068.2025.2498460","DOIUrl":"https://doi.org/10.1080/10826068.2025.2498460","url":null,"abstract":"<p><p>In this study, the kinetic properties of polyphenol oxidase (PPO) extracted from <i>Satureja cuneifolia</i> were investigated using catechol and 4-methylcatechol as substrates. Optimal pH and temperature values were determined at each purification step. Subsequently, the optimum immobilization conditions were established as 2 hours of stirring time and 0.05 g of multi-walled carbon nanotubes (MWCNTs). Characterization by BET, FTIR, DTA/TG, TEM, and SEM/EDX analyses confirmed the successful immobilization of PPO onto mesoporous MWCNTs, with notable changes in surface morphology and thermal degradation behavior. The optimum pH for the free enzyme remained constant across purification methods but varied with the substrate, while the optimum temperature was consistently found at 30 °C. Upon immobilization, the optimum temperature shifted to higher values, indicating enhanced thermal stability. Catalytic efficiency (Vmax/K_M) for catechol decreased significantly after immobilization (from 2.5 × 10⁶ to 5 × 10⁴ min^-1), whereas for 4-methylcatechol, the immobilized enzyme retained a high catalytic efficiency (Vmax/K_M =1 × 10⁶ min^-1), comparable to that of the free enzyme. This shift suggests that immobilization favored substrate specificity toward 4-methylcatechol. Overall, the MWCNT-PPO system demonstrated enhanced stability, improved reusability, and altered substrate selectivity, making it a strong candidate for industrial biocatalytic applications where operational durability and efficiency are critical.</p>","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}