Preparative Biochemistry & Biotechnology最新文献_第2页

Quantification of human intravenous immunoglobulin from plasma and in process samples by affinity chromatography. 利用亲和层析法对血浆和加工样品中的人静脉注射免疫球蛋白进行定量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-02 DOI: 10.1080/10826068.2024.2384494

Markus Mozgovicz, Nico Lingg, Igor Tadeu Lazzarotto Bresolin, Theresa Schaufler, Alois Jungbauer

{"title":"Quantification of human intravenous immunoglobulin from plasma and in process samples by affinity chromatography.","authors":"Markus Mozgovicz, Nico Lingg, Igor Tadeu Lazzarotto Bresolin, Theresa Schaufler, Alois Jungbauer","doi":"10.1080/10826068.2024.2384494","DOIUrl":"10.1080/10826068.2024.2384494","url":null,"abstract":"Advances in affinity chromatography now make it possible to analyze immunoglobulin G from plasma and its fractions with a simple chromatographic method. Ligands derived from camelid antibodies have been developed which have affinity to all 4 subclasses of human IgG without a cross reactivity to other immunoglobulins. The commercially available Capture Select FcXL is the basis for a simple method for direct quantification of immunoglobulin G from plasma or from fractions from cold ethanol precipitation. After direct injection of the sample into the column the unbound proteins are washed out with equilibration buffer and eluted with a pH-step. The elution the peak is integrated, and quantity is derived form a standard curve. The limit of detection with 40 µg/mL, and a linearity up to 250 µg/mL allows an analysis of samples ranging from 0.04 to 50 mg/mL using varying injection volume without further dilution and the two-wavelength detection. A full cycle is completed within five minutes. This method can serve as orthogonal method for in-process control but also for process development.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"217-222"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the extraction process of total steroids from Phillinus gilvus (Schwein.) Pat. by artificial neural network (ANN)-response surface methodology and identification of extract constituents. 用人工神经网络-响应面方法优化从Phillinus gilvus (Schwein.) Pat.中提取总甾体的工艺并鉴定提取物成分。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-23 DOI: 10.1080/10826068.2024.2394449

Xusheng Gao, Junxia Ma, Fengfu Li, Qian Zhou, Dan Gao

{"title":"Optimization of the extraction process of total steroids from Phillinus gilvus (Schwein.) Pat. by artificial neural network (ANN)-response surface methodology and identification of extract constituents.","authors":"Xusheng Gao, Junxia Ma, Fengfu Li, Qian Zhou, Dan Gao","doi":"10.1080/10826068.2024.2394449","DOIUrl":"10.1080/10826068.2024.2394449","url":null,"abstract":"Phillinus gilvus (Schwein.) Pat has pharmacological effects such as tonifying the spleen, dispelling dampness, and strengthening the stomach, in which sterol is one of the main compounds of P. gilvus, but there has not been thought you to its extraction and detailed identification of its composition, in the present study, we used artificial neural network (ANN) and response surface methodology (RSM) to optimize the conditions of ultrasonic-assisted extraction, and the parameters of the independent and interaction effects were evaluated. Ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used to identify the major components in the purified extract. The results showed that the optimal extraction process conditions were: ultrasonic time 96 min, ultrasonic power 140 W, liquid to material ratio 1:25 g/ml, and ultrasonic temperature 30.7 °C. The compliance rates of the predicted and experimental values for the artificial neural network model and the response surface model were 98.3% and 96.12%, respectively, indicating that both models have the potential to be used for optimizing the extraction process of P. gilvus in industry. A total of 120 compounds and 30 major steroids were identified by comparison with the reference compounds. Among the major steroidal components are these findings will contribute to the isolation and utilization of active ingredients in P. gilvus.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"230-243"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142044100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems. 在微生物宿主系统中可溶性表达重组抗体片段的最新进展。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-28 DOI: 10.1080/10826068.2024.2394446

Preeti Saroha, Rucha S Patil, Anurag S Rathore

{"title":"Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems.","authors":"Preeti Saroha, Rucha S Patil, Anurag S Rathore","doi":"10.1080/10826068.2024.2394446","DOIUrl":"10.1080/10826068.2024.2394446","url":null,"abstract":"Recombinant fabs dominate the pharmaceutical pipelines today with microbial host systems continuing to be a major contributor toward their production. Escherichia coli is a versatile host for recombinant protein expression due to its simplicity, affordability, and ability to be cultivated at high cell density. It is particularly suitable for non-glycosylated proteins and small proteins. Despite the aforementioned benefits, the use of E. coli as the host for the synthesis of recombinant antibody fragments often suffers from low yield and reduced activity. In most cases, proteins are expressed as inclusion bodies and need to undergo refolding to achieve their active forms and this refolding step is generally low-yielding. In this article, we review the various approaches that researchers have taken to enhance the production of recombinant antibody fragments in E. coli. Molecular biology-oriented approaches such as cloning, chaperone-mediated folding, and host cell screening as well as process optimization involving examination of process parameters, media, and feeding have been addressed.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"131-140"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142086245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 DOI: 10.1080/10826068.2025.2457545

Pankaj D Parab, Rakhee D Khandeparker

{"title":"Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential.","authors":"Pankaj D Parab, Rakhee D Khandeparker","doi":"10.1080/10826068.2025.2457545","DOIUrl":"https://doi.org/10.1080/10826068.2025.2457545","url":null,"abstract":"Microbial xylanases are xylan hydrolyzing enzymes which has congregated attention due to their immense potential in many industries. The current study is focused on xylanase from bacterial isolate identified as Bacillus sp. NIORKP76 on an inexpensive agro-industrial waste (wheat bran) under solid-state fermentation. The isolate showed maximum xylanase production in growth medium supplemented with phosphate, NaCl and NH4Cl at 64 mM, 15 mg/mL and 0.3 mg/mL respectively which was maintained at 1:3 substrate to moisture ratio (w/v). Using optimized conditions, the maximum xylanase production in wheat bran was achieved in 72 h at room temperature 28 ± 2 °C. Xylanase exhibited pH optimum of 8.0 and retained 92% of its residual activity after 24 h incubation at pH 8. Xylanase displayed optimum activity at 60 °C, whereas it remained unhindered even after 12 h of incubation period at 30 and 40 °C. The xylanase isolated in this study was purified to homogeneity with a molecular weight of approximately 28 kDa. Xylanase from Bacillus sp. NIORKP76 was potentially converting agro-residues into biofuel feedstock. Pretreated wheat bran produced the highest yield fermentable sugars (141 mg/g), after 8 h incubation with 5 U/g xylanase and at 40 °C.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the production of L-proline in recombinant Escherichia coli BL21 by metabolic engineering. 通过代谢工程提高重组大肠杆菌 BL21 中 L-脯氨酸的产量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-10 DOI: 10.1080/10826068.2024.2378104

Jiajie Lu, Bing Fu, Zhiwen Zhu, Chuyang Yan, Fuyao Guan, Peize Wang, Ping Yu

引用次数: 0

Highly efficient strategy of lipopolysaccharide (LPS) decontamination from rHBsAg: synergistic effect of enhanced magnetic nanoparticles (MNPs) as an LPS affinity adsorbent (LAA) and surfactant as a dissociation factor. 从 rHBsAg 中清除脂多糖（LPS）的高效策略：增强型磁性纳米颗粒（MNPs）作为 LPS 亲和吸附剂（LAA）和表面活性剂作为解离因子的协同效应。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-13 DOI: 10.1080/10826068.2024.2377326

Alireza Kavianpour, Seyed Nezamedin Hosseini, Mohsen Ashjari, Maryam Khatami, Taravatsadat Hosseini, Hosnsa Soleimani

{"title":"Highly efficient strategy of lipopolysaccharide (LPS) decontamination from rHBsAg: synergistic effect of enhanced magnetic nanoparticles (MNPs) as an LPS affinity adsorbent (LAA) and surfactant as a dissociation factor.","authors":"Alireza Kavianpour, Seyed Nezamedin Hosseini, Mohsen Ashjari, Maryam Khatami, Taravatsadat Hosseini, Hosnsa Soleimani","doi":"10.1080/10826068.2024.2377326","DOIUrl":"10.1080/10826068.2024.2377326","url":null,"abstract":"The interaction of lipopolysaccharide with a recombinant protein is a serious bottleneck, particularly in the purification step of bioprocessing. Recombinant hepatitis B surface antigen (rHBsAg), the active ingredient of the hepatitis B vaccine, is probably contaminated by extrinsic LPS like other biopharmaceuticals. This research intends to eliminate LPS from its mixture with rHBsAg efficiently. Immobilized polymyxin B on magnetic nanoparticles (PMB-MNPs) was synthesized and implemented as an enhanced LPS affinity adsorbent (LAA). The 20-80 EU/dose binary samples with and without surfactant were applied to PMB-MNPs. Formerly, dynamic light scattering (DLS) and transmission electron microscopy (TEM) were examined on the samples to qualitatively show the dissociation effect of the surfactant. Considering the high potential interaction of LPS with HBsAg, the dissociation effects of 0.5 and 1.5% Tween 20 on the binary samples were assessed using immunoaffinity chromatography (IAC) as a quantification tool. The dissociation effect of Tween 20 substantially diminished the interaction, leading to a proportional increase of free LPS up to 66%. The synergetic effect of Tween 20 and privileged LAA was highly effective in eliminating more than 80% of LPS with a remarkable LPS clearance factor of 5.8 and a substantial protein recovery rate of 97%.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"150-159"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial neural networks (ANN)-genetic algorithm (GA) optimization on thermosonicated achocha juice: kinetic and thermodynamic perspectives of retained phytocompounds. 人工神经网络（ANN）-遗传算法（GA）对热渗阿楚茶汁的优化：保留植物化合物的动力学和热力学视角。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-12 DOI: 10.1080/10826068.2024.2378101

Puja Das, Prakash Kumar Nayak, Radha Krishnan Kesavan

{"title":"Artificial neural networks (ANN)-genetic algorithm (GA) optimization on thermosonicated achocha juice: kinetic and thermodynamic perspectives of retained phytocompounds.","authors":"Puja Das, Prakash Kumar Nayak, Radha Krishnan Kesavan","doi":"10.1080/10826068.2024.2378101","DOIUrl":"10.1080/10826068.2024.2378101","url":null,"abstract":"The extraction of phytocompounds from Achocha (Cyclanthera pedata) vegetable juice using traditional methods often results in suboptimal yields and efficiency. This study aimed to enhance the extraction process through the application of thermosonication (TS). To achieve this, an artificial neural network (ANN) and a genetic algorithm (GA) were utilized to simulate and optimize the process parameters. The study investigated the influence of ultrasonic amplitude (30%-50%), temperature (30 °C-50 °C), and sonication duration (15-60 min) on total polyphenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AOA), and ascorbic acid content (AA). Remarkably, the ANN-GA optimization resulted in optimal TS conditions: an ultrasonic amplitude of 40%, a temperature of 40 °C, and a sonication duration of 30 min. Subsequent analysis of extraction kinetics and thermodynamics across various temperatures (30 °C-50 °C) and extraction times (0-30 min) demonstrated R2 (0.98821) and χ2 (1.74773) for TPC with activation energy (Ea) 26.0456, R2 (0.99906) and χ2 (0.07215) for TFC with Ea 26.2336, R2 (0.99867) and χ2 (0.03003) for AOA with Ea 26.0987, R2 (0.99731) and χ2 (0.13719) for AA with Ea 26.0984, validating the pseudo second-order kinetic model. These findings indicate that increased temperature enhances the saturation concentration and rate constant of phytochemical extraction.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"171-186"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of lyoprotectant for recombinant human acidic fibroblast growth factor by response surface methodology. 用响应面方法优化重组人酸性成纤维细胞生长因子的冻干保护剂。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-19 DOI: 10.1080/10826068.2024.2378098

Qiwen Huang, Jing Hu, Dongjie Mei, Guopan Li, Jun Rong

{"title":"Optimization of lyoprotectant for recombinant human acidic fibroblast growth factor by response surface methodology.","authors":"Qiwen Huang, Jing Hu, Dongjie Mei, Guopan Li, Jun Rong","doi":"10.1080/10826068.2024.2378098","DOIUrl":"10.1080/10826068.2024.2378098","url":null,"abstract":"Recombinant human acidic fibroblast growth factor (rh-aFGF) is a widely used biological product, but it is unstable and its biological activity is easy to decrease. In order to maintain the long-term stability and biological activity of rh-aFGF, based on the response surface method, the freeze-drying characterization and cell proliferation rate of rh-aFGF freeze-dried powder were evaluated by scoring and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay in this study. The optimal concentrations of trehalose, glycine and BSA were optimized, and the optimal formulation was verified by regression experiment. The results showed that trehalose, glycine and BSA had significant effects on the characterization of lyophilized rh-aFGF and cell proliferation. The optimal formulation of 5.7% trehalose, 2.04% glycine and 1.98%BSA combined with rh-aFGF could achieve the optimal freeze-dried characterization and biological activity. Using the best formulation to verify, the freeze-dried formability index of the freeze-dried powder was 23.35, and the rate of cell proliferation was 43.59%, which was close to the expected 23 and 41.69%. This study determined a freeze-dried formulation of rh-aFGF that meets the requirements of freeze-dried formalization integrity and maintains biological activity, providing reliable support for the subsequent development of related drugs.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"160-170"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141724341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides.

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 DOI: 10.1080/10826068.2024.2441914

Yao Wen, Xiangyi Yan, Haiyun Chen

{"title":"Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides.","authors":"Yao Wen, Xiangyi Yan, Haiyun Chen","doi":"10.1080/10826068.2024.2441914","DOIUrl":"https://doi.org/10.1080/10826068.2024.2441914","url":null,"abstract":"For the valorization of Eucommia ulmoides (EU) for the functional food industry, the process of ultrasonic-assisted deep eutectic solvent (DES) extraction of EU polysaccharides (EUP) was optimized by response surface methodology. After response surface analysis and experimental verification, the optimum extraction conditions were as follows: the molar ratio of choline chloride to oxalic acid was 0.9:1, the water content of DES was 40.28%, the ratio of material to liquid was 1:22 g/mL, the time was 90 min, and the power was 320 W. The extraction rate of EUP was 1.71%, which was higher than the extraction rate of 0.75% by ultrasonic water extraction under the same conditions, and the optimization effect was better. After DEAE column gradient elution and Sephadex G-75 gel column chromatography, the elution curve and a refined polysaccharide (EUP-1) were obtained. The IC50 values of EUP-1 against α-glucosidase and α-amylase were 0.091 and 0.011 mg/mL. The IC50 values of EUP-1 on DPPH and ABTS·+ scavenging were 0.065 and 0.065 mg/mL. The monosaccharide composition of EUP-1 was analyzed as mannose, rhamnose, glucuronic acid, and galacturonic acid in the following molar ratio: 1.00: 1.28: 1.53: 1.88.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The improved purification technique for isolation of the novel CGTase from the alkaliphilc strain Caldalkalibacillus mannanilyticus IB-OR17-B1. 从嗜碱性菌株 Caldalkalibacillus mannanilyticus IB-OR17-B1 中分离新型 CGT 酶的改良纯化技术。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-06 DOI: 10.1080/10826068.2024.2386558

P Yu Milman, E A Gilvanova, G E Aktuganov

{"title":"The improved purification technique for isolation of the novel CGTase from the alkaliphilc strain Caldalkalibacillus mannanilyticus IB-OR17-B1.","authors":"P Yu Milman, E A Gilvanova, G E Aktuganov","doi":"10.1080/10826068.2024.2386558","DOIUrl":"10.1080/10826068.2024.2386558","url":null,"abstract":"Cyclodextrin-glucanotransferase (CGTase, EC 2.4.1.19) is a multifunctional enzyme that catalyzes many enzymatic reactions including cyclization, binding, disproportionation and hydrolysis reactions, playing an important role in the enzymatic synthesis of compounds that are widely used in agriculture, pharmaceuticals, food, chemical and biotechnology industries. The present research is aimed to optimize the purification protocol for the extracellular CGTase of alkaliphilc bacterial strain Caldalkalibacillus mannanilyticus IB-OR17-B1 guaranteeing the enzyme homogeneity and its high yield. The improved combination of ultrafiltration and corn-starch (5% w/v) affinity sorption techniques resulted to mild and rapid isolation of electrophoritically homogenic enzyme at 18 × increase of its specific activity and yield 56%. The developed two-step procedure instead the practiced tree-step one using commonly ion-exchange chromatography as final purification technique highly contributes in advance of cost-effectiveness for industrial production and isolation of valuable CGTases.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"223-229"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0