Preparative Biochemistry & Biotechnology最新文献_第2页

Immobilization, optimization, characterization and kinetic properties of polyphenol oxidase to multi-walled carbon nanotube. 多壁碳纳米管多酚氧化酶的固定化、优化、表征及动力学性质研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-05-05 DOI: 10.1080/10826068.2025.2498460

Şeyma Çam, Mehmet Doğan, Pınar Turan Beyli, Serap Doğan, Zeynep Bicil, Berna Koçer Kızılduman

{"title":"Immobilization, optimization, characterization and kinetic properties of polyphenol oxidase to multi-walled carbon nanotube.","authors":"Şeyma Çam, Mehmet Doğan, Pınar Turan Beyli, Serap Doğan, Zeynep Bicil, Berna Koçer Kızılduman","doi":"10.1080/10826068.2025.2498460","DOIUrl":"https://doi.org/10.1080/10826068.2025.2498460","url":null,"abstract":"In this study, the kinetic properties of polyphenol oxidase (PPO) extracted from Satureja cuneifolia were investigated using catechol and 4-methylcatechol as substrates. Optimal pH and temperature values were determined at each purification step. Subsequently, the optimum immobilization conditions were established as 2 hours of stirring time and 0.05 g of multi-walled carbon nanotubes (MWCNTs). Characterization by BET, FTIR, DTA/TG, TEM, and SEM/EDX analyses confirmed the successful immobilization of PPO onto mesoporous MWCNTs, with notable changes in surface morphology and thermal degradation behavior. The optimum pH for the free enzyme remained constant across purification methods but varied with the substrate, while the optimum temperature was consistently found at 30 °C. Upon immobilization, the optimum temperature shifted to higher values, indicating enhanced thermal stability. Catalytic efficiency (Vmax/KM) for catechol decreased significantly after immobilization (from 2.5 × 106 to 5 × 104 min-1), whereas for 4-methylcatechol, the immobilized enzyme retained a high catalytic efficiency (Vmax/KM =1 × 106 min-1), comparable to that of the free enzyme. This shift suggests that immobilization favored substrate specificity toward 4-methylcatechol. Overall, the MWCNT-PPO system demonstrated enhanced stability, improved reusability, and altered substrate selectivity, making it a strong candidate for industrial biocatalytic applications where operational durability and efficiency are critical.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic hydrolysis of lignocellulosic biomass using co-immobilization of tri-enzymes on chitosan-magnetite nanoparticle beads. 利用壳聚糖-磁铁矿纳米颗粒珠共固定化三酶协同水解木质纤维素生物质。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-30 DOI: 10.1080/10826068.2025.2496255

Sushil Nagar, Meena Sindhu, Kajal Kumari, Vinay Kumar, Gulab Singh, Neeraj Kharor, Vishal Chugh, Vinod Kumar

{"title":"Synergistic hydrolysis of lignocellulosic biomass using co-immobilization of tri-enzymes on chitosan-magnetite nanoparticle beads.","authors":"Sushil Nagar, Meena Sindhu, Kajal Kumari, Vinay Kumar, Gulab Singh, Neeraj Kharor, Vishal Chugh, Vinod Kumar","doi":"10.1080/10826068.2025.2496255","DOIUrl":"https://doi.org/10.1080/10826068.2025.2496255","url":null,"abstract":"This paper presents the co-immobilization of three enzymes-laccase, cellulase, and xylanase-on chitosan-magnetite nanoparticle beads, with process parameters optimized using response surface methodology on glutaraldehyde-activated chitosan-magnetite beads. The optimization achieved an impressive immobilization yield of 95.25%. Following immobilization on chitosan-magnetite beads (CMBs), the kinetic properties (Km and Vmax), as well as the optimal pH and temperature, were significantly enhanced. The immobilized LCX demonstrated excellent reusability, maintaining 51% of its initial activity after five consecutive cycles, and could be easily recovered using an external magnet. Maximum digestibility of cellulose (% Dc), hemicellulose (% DH), and lignin (% DL) was observed when 10 g of pretreated wheat bran was treated with 20 LCX-loaded CMBs at 40 °C for 60 minutes. The digestibility values for cellulose, hemicellulose, and lignin were 42.10 ± 1.85%, 52.30 ± 2.05%, and 18.12 ± 0.96%, respectively, using immobilized LCX-CMBs-1.0 to 1.5 times higher than those obtained with free enzymes. Additionally, the yield of reducing sugars was 62.17% for immobilized LCX compared to 46.06% for free LCX. The immobilization on CMBs offers an easily removable and cost-effective solution for various industrial applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of GABA producing strains from sourdoughs and optimization of GABA production by response surface-integrated particle swarm approach. 响应面集成粒子群方法在酵母中GABA产菌鉴定及GABA产菌优化中的应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-30 DOI: 10.1080/10826068.2025.2498456

Zuhal Alkay, Abdullah Tahir Şensoy, Muhammed Zeki Durak, Yunus Emre Tuncil, Enes Dertli

{"title":"Identification of GABA producing strains from sourdoughs and optimization of GABA production by response surface-integrated particle swarm approach.","authors":"Zuhal Alkay, Abdullah Tahir Şensoy, Muhammed Zeki Durak, Yunus Emre Tuncil, Enes Dertli","doi":"10.1080/10826068.2025.2498456","DOIUrl":"https://doi.org/10.1080/10826068.2025.2498456","url":null,"abstract":"Lactic Acid Bacteria (LAB) strains were isolated from sourdough samples collected from five provinces of Türkiye and the Cyprus region. The gamma-aminobutyric acid (GABA) production ability of these LAB strains with (+) gad gene regions was evaluated. Response Surface Method (RSM) and Particle Swarm Optimization (PSO) techniques were used together to optimize the GABA environment of Levilactobacillus brevis SD48 strain with the highest GABA production. Optimization of fermentation conditions for GABA production of Levilactobacillus brevis SD48 strain was designed with Box-Behnken design. Optimization parameters tested were pH, temperature, time and monosodium glutamate (MSG) concentration. Fermentation time (24, 48, 72 hours), pH values (4.5, 5.75 and 7.0), temperature (30, 35 and 40 °C), MSG concentration (50, 225, 400 mM) were taken. It was seen that the best process parameters that allow maximum GABA yield should be temperature 30 °C, MSG concentration 130.71 mM, pH 4.74 and time 72 hour. According to the results, it can be thought that microbial production of GABA is both cheap and the optimized conditions we determined will play an important role in the production of GABA-rich fermented foods.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system. 热敏聚合物NPE-108/水双水相萃取螺旋霉素的分子动力学模拟与验证。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-29 DOI: 10.1080/10826068.2025.2478402

Xiaotong Zheng, Ting Zheng, Wen Wang, Xuejun Cao, Junfen Wan

{"title":"Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system.","authors":"Xiaotong Zheng, Ting Zheng, Wen Wang, Xuejun Cao, Junfen Wan","doi":"10.1080/10826068.2025.2478402","DOIUrl":"https://doi.org/10.1080/10826068.2025.2478402","url":null,"abstract":"Spiramycin is a 16-membered macrolide antibiotic widely used in the medical field. Industrial extraction of antibiotics from fermentation broth using organic solvents raises various environmental and health concerns. In this study, a thermosensitive polymer, NPE-108, was used to construct an aqueous two-phase system (ATPS) for the extraction of spiramycin. We used Gromacs software to develop a molecular dynamics simulation model to reveal the distribution mechanism of spiramycin molecules in the NPE-108/water ATPS from a microscopic perspective. Additionally, we examined the effects of volume ratio, temperature, and pH on extraction through experimentation. Under the optimal conditions for forward extraction, the distribution coefficient and extraction efficiency were 25.3 and 89.9%, respectively. Under the optimal conditions for back extraction, the distribution coefficient and extraction efficiency were 6.8 and 81.5%, respectively. Optimization of crystallization conditions resulted in a crystal yield of 88.1% and a purity of 98.4%. The content of both spiramycin components and impurities in the crystalline sample met the requirements of the European Pharmacopeia. The results of this study provided insights into molecular interactions and the extraction process, offering a more environmentally friendly and economically viable alternative for industrial spiramycin production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":2.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of medical-grade biopolymer in air lift bioreactors. 在气升式生物反应器中生产医用级生物聚合物。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-25 DOI: 10.1080/10826068.2025.2496246

Navodit Kumar Singh, Ashok Kumar Srivastava, T R Sreekrishnan, Srividya Shivakumar

{"title":"Production of medical-grade biopolymer in air lift bioreactors.","authors":"Navodit Kumar Singh, Ashok Kumar Srivastava, T R Sreekrishnan, Srividya Shivakumar","doi":"10.1080/10826068.2025.2496246","DOIUrl":"https://doi.org/10.1080/10826068.2025.2496246","url":null,"abstract":"Microbes are known to produce biopolymers for societal applications. Economical production of biopolymer (PHB) is desperately required to significantly replace or reduce usage of non-degradable polypropylene produced by disappearing petroleum resources. Besides it is also equally important to ensure abundant availability of low cost medical grade biopolymers which can be used for several medical applications in society. It has been invariably observed that mechanical agitation in the bioreactors features major power consumption in the operation of bioreactors therefore usage of air lift bioreactors are likely to reduce power consumption by mechanical agitation significantly thereby leading to economic biopolymer production. Present investigation evaluates the possible role of pneumatic bioreactors (e.g., Bubble Column, Outer Aeration Inner Settling, Inner Aeration Outer Settling) as alternates to mechanically agitated bioreactors for the economic production of medical grade biopolymers P(3HB) by Bacillus thuringiensis IAM12077 using glycerol and glucose as major substrates. It was observed that Bacillus thuringiensis IAM12077 cultivations featured Biopolymer P(3HB) accumulations of 22.48%, 37.07%, 27.73%, in BC, OAIS, IAOS air lift bioreactors. Relatively higher product yield, volumetric productivity and P(3HB) accumulation was observed in Outer Aeration Inner Settling (OAIS) air lift bioreactor configuration as opposed to other pneumatic bioreactors.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing cellulase performance through nanomaterials and MOFs: innovations and applications. 通过纳米材料和mof提高纤维素酶的性能：创新和应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-23 DOI: 10.1080/10826068.2025.2494105

Shashi Suhag, Poonam Yadav, Veena Sachdeva, Khushi Lohan, Vijeta Luhach, Vinita Hooda

{"title":"Enhancing cellulase performance through nanomaterials and MOFs: innovations and applications.","authors":"Shashi Suhag, Poonam Yadav, Veena Sachdeva, Khushi Lohan, Vijeta Luhach, Vinita Hooda","doi":"10.1080/10826068.2025.2494105","DOIUrl":"https://doi.org/10.1080/10826068.2025.2494105","url":null,"abstract":"Cellulase is widely utilized in industries such as biofuel production, food processing, textiles, and waste management due to its catalytic efficiency in breaking down cellulose. However, its industrial application is limited by instability under harsh conditions. This review examines innovative methodologies for enhancing cellulase performance through immobilization on nanomaterials, including magnetic nanoparticles, carbon-based nanomaterials, and metal-organic frameworks (MOFs). Immobilization techniques, such as adsorption, covalent bonding, and cross-linking, have been shown to significantly improve cellulase stability, activity, and reusability. Key findings include a threefold increase in catalytic efficiency when cellulase is immobilized on magnetic nanoparticles, alongside notable enhancements in thermal stability when employing MOF composites. Despite these advancements, challenges such as enzyme leakage, material costs, and scalability remain. Future opportunities lie in developing more cost-effective, scalable immobilization strategies, with interdisciplinary approaches offering the potential to further enhance enzyme efficiency across diverse application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-22"},"PeriodicalIF":2.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A strategy of extracting and purifying the α-terpineol obtained from the Penicillium digitatum biotransformation of limonene. 指状青霉生物转化柠檬烯所得α-松油醇的提取纯化策略。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-22 DOI: 10.1080/10826068.2025.2494102

Zehao Li, Zixuan Wang, Lulu Zhang, Feng Yingjie, Jinchu Yang, Haoliang Li, Weimin Song, Qiuling Wang, Yongfeng Yang, Zhenzhen Huang, Yongming Xu, Yufeng Fu, Meizhou Ding, Gang Fan, Jingnan Ren, Aiqun Yu, Jian Feng, Guijie Li, Linhua Huang, Yujiao Cheng

{"title":"A strategy of extracting and purifying the α-terpineol obtained from the Penicillium digitatum biotransformation of limonene.","authors":"Zehao Li, Zixuan Wang, Lulu Zhang, Feng Yingjie, Jinchu Yang, Haoliang Li, Weimin Song, Qiuling Wang, Yongfeng Yang, Zhenzhen Huang, Yongming Xu, Yufeng Fu, Meizhou Ding, Gang Fan, Jingnan Ren, Aiqun Yu, Jian Feng, Guijie Li, Linhua Huang, Yujiao Cheng","doi":"10.1080/10826068.2025.2494102","DOIUrl":"https://doi.org/10.1080/10826068.2025.2494102","url":null,"abstract":"α-Terpineol, an abundant oxygenated monoterpene compound, shows a varied range of beneficial bioactivities. This study focused on the efficient extraction and purification of α-terpineol from the biotransformation of limonene using Penicillium digitatum DSM 62840 mutant (PdTP1-overexpressed OE2 strain). The α-terpineol was primarily distributed in the supernatant, with minimal association with the biomass. And the optimal extraction was achieved using ethyl acetate as the extractant, which can directly obtain the highest recovery of α-terpineol without sequential extractions. The equilibrium of mass transfer was quickly reached (≤20 s in vortex). Additionally, the purification method of α-terpineol using column chromatography was described, further improving the purity of product to 96.86% in gas chromatography. Structural identification of purified α-terpineol was confirmed using gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance. This simplified and efficient strategy for the extraction and purification of α-terpineol not only provides a solid theoretical basis for the final step in the relevant study of microbial synthesis of α-terpineol but also is of great significance for its industrial application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, and application on inulin hydrolysis. 土曲霉URM4658菊粉酶在海藻酸钙微球中的固定化、生化特性和动力学/热力学参数的评价及其在菊粉水解中的应用

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-21 DOI: 10.1080/10826068.2025.2486424

Wallace Ribeiro da Silva, Camila Fernanda de Aquino Luna, Joyce Gueiros Wanderley Siqueira, Jorge Vinícius Fernandes Lima Cavalcanti, Rodrigo Lira de Oliveira, Tatiana Souza Porto

{"title":"Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, and application on inulin hydrolysis.","authors":"Wallace Ribeiro da Silva, Camila Fernanda de Aquino Luna, Joyce Gueiros Wanderley Siqueira, Jorge Vinícius Fernandes Lima Cavalcanti, Rodrigo Lira de Oliveira, Tatiana Souza Porto","doi":"10.1080/10826068.2025.2486424","DOIUrl":"https://doi.org/10.1080/10826068.2025.2486424","url":null,"abstract":"The present study aimed to immobilize an inulinase obtained from Aspergillus terreus URM4658 by entrapment in calcium alginate beads. The immobilization process yielded a satisfactory yield (92.72%) using 1.25% sodium alginate and 0.35 M CaCl2 with a curing time of 90 min. The immobilized enzyme exhibited optimum pH and temperature at 7.0 and 60 °C, respectively, showing an increased affinity for the substrate after the immobilization process, as evidenced by the decrease in Km compared to its free form. Moreover, the immobilized inulinase demonstrated good thermostability at 50 and 60 °C, as observed from the t1/2 (649.83-420.84 min) and D-values (2158.67-1398.00 min), respectively. The immobilized biocatalyst also exhibited good reusability, maintaining 92.73% of residual activity after 10 reaction cycles and no loss of activity after 30 days of storage. A continuous inulin hydrolysis operation in a packed bed reactor was performed using the immobilized inulinase, and a maximum release of total reducing sugars and nystose of 3.27 and 0.82 g L-1, respectively, was observed. The results indicate that an immobilized biocatalyst is a promising alternative for bioprocess involving inulin-rich feedstocks.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step rapid production of DNA polymerase from Pyrococcus furiosus in Escherichia coli system under optimized culture conditions. 优化培养条件下，在大肠杆菌体系中一步快速生产荧光焦球菌DNA聚合酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-17 DOI: 10.1080/10826068.2025.2483238

Fina Amreta Laksmi, Mulyorini Rahayuningsih, Arfena Rizqi Amalia, Wiga Alif Violando, Isa Nuryana, Yogi Ertanto, Yudhi Nugraha

{"title":"One-step rapid production of DNA polymerase from Pyrococcus furiosus in Escherichia coli system under optimized culture conditions.","authors":"Fina Amreta Laksmi, Mulyorini Rahayuningsih, Arfena Rizqi Amalia, Wiga Alif Violando, Isa Nuryana, Yogi Ertanto, Yudhi Nugraha","doi":"10.1080/10826068.2025.2483238","DOIUrl":"https://doi.org/10.1080/10826068.2025.2483238","url":null,"abstract":"The Pyrococcus furiosus (Pfu) DNA polymerase is an enzyme widely used in PCR due to its high fidelity, thermal stability, and ability to amplify even minute amounts of DNA with exceptional specificity and sensitivity. This study addresses the growing demand for efficient and cost-effective production of Pfu polymerase by optimizing its recombinant expression in Escherichia coli BL21 star (DE3) using a synthetic gene in the pD451-SR plasmid. Key parameters, including IPTG concentration (0.4 mM), post-induction incubation time (12 h), and cell density prior to induction (OD600 of 0.4), were systematically optimized to maximize yield and activity. A rapid and straightforward purification protocol was developed, utilizing a boiling-mediated lysis method that effectively purified Pfu polymerase within 20 minutes. A total of 795 mg/L of pure Pfu polymerase under optimized conditions, led to a 30-fold increase in protein yield with an enzyme activity of 2210 units, with SDS-PAGE and western blot analyses confirming a single protein band at 90 kDa. The optimized production and purification methods significantly enhance the time and cost-efficiency of Pfu polymerase production, making it highly suitable for industrial-scale applications. This streamlined approach positions Pfu polymerase as a valuable tool for routine PCR, cloning, mutagenesis, and advanced applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decayed fruit waste juice as a sustainable medium for high-yield microbial cellulose production: optimization, characterization and bioactivity studies. 作为高产微生物纤维素生产的可持续培养基的腐烂果渣汁：优化、表征和生物活性研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-09 DOI: 10.1080/10826068.2025.2489535

Sheetal Singh, Isha Santhosh, Subbalaxmi Selvaraj, Sowmya R Holla

{"title":"Decayed fruit waste juice as a sustainable medium for high-yield microbial cellulose production: optimization, characterization and bioactivity studies.","authors":"Sheetal Singh, Isha Santhosh, Subbalaxmi Selvaraj, Sowmya R Holla","doi":"10.1080/10826068.2025.2489535","DOIUrl":"10.1080/10826068.2025.2489535","url":null,"abstract":"In the present study, microbial cellulose (MC) was produced from decayed fruit waste juice as a fermentation medium using a consortium of microbes grown on vegetable and fruit waste as the inoculum. To optimize the MC yield, the central composite design (CCD) of response surface methodology (RSM) was applied. Four factors at five different levels were chosen in the design with a total of 26 experimental runs obtained from the CCD design. The optimal conditions were fruit waste extract (60% v/v), glucose concentration (0.75% w/v), inoculum size (5% v/v), and fermentation time (5 d) which displayed a 2.1-fold increase in MC yield of 21.719 g/L. The MC was characterized using Fourier Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy, and Thermogravimetric; and compared its properties to cellulose produced through other techniques. The produced MC was examined for its antioxidant properties using cyclic voltammetry (CV) displaying an anodic peak at 1.15 V. Lastly, the antimicrobial activity was tested against four different microbial strains. Among them, MC exhibited a clear zone of 7.661 ± 0.256 cm2 against E. coli. The results of this study concluded that decayed fruit waste juice is a sustainable and economical fermentation medium.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0