Preparative Biochemistry & Biotechnology最新文献_第2页

Enhancing cellulase performance through nanomaterials and MOFs: innovations and applications. 通过纳米材料和mof提高纤维素酶的性能：创新和应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-23 DOI: 10.1080/10826068.2025.2494105

Shashi Suhag, Poonam Yadav, Veena Sachdeva, Khushi Lohan, Vijeta Luhach, Vinita Hooda

{"title":"Enhancing cellulase performance through nanomaterials and MOFs: innovations and applications.","authors":"Shashi Suhag, Poonam Yadav, Veena Sachdeva, Khushi Lohan, Vijeta Luhach, Vinita Hooda","doi":"10.1080/10826068.2025.2494105","DOIUrl":"https://doi.org/10.1080/10826068.2025.2494105","url":null,"abstract":"Cellulase is widely utilized in industries such as biofuel production, food processing, textiles, and waste management due to its catalytic efficiency in breaking down cellulose. However, its industrial application is limited by instability under harsh conditions. This review examines innovative methodologies for enhancing cellulase performance through immobilization on nanomaterials, including magnetic nanoparticles, carbon-based nanomaterials, and metal-organic frameworks (MOFs). Immobilization techniques, such as adsorption, covalent bonding, and cross-linking, have been shown to significantly improve cellulase stability, activity, and reusability. Key findings include a threefold increase in catalytic efficiency when cellulase is immobilized on magnetic nanoparticles, alongside notable enhancements in thermal stability when employing MOF composites. Despite these advancements, challenges such as enzyme leakage, material costs, and scalability remain. Future opportunities lie in developing more cost-effective, scalable immobilization strategies, with interdisciplinary approaches offering the potential to further enhance enzyme efficiency across diverse application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-22"},"PeriodicalIF":2.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A strategy of extracting and purifying the α-terpineol obtained from the Penicillium digitatum biotransformation of limonene. 指状青霉生物转化柠檬烯所得α-松油醇的提取纯化策略。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-22 DOI: 10.1080/10826068.2025.2494102

Zehao Li, Zixuan Wang, Lulu Zhang, Feng Yingjie, Jinchu Yang, Haoliang Li, Weimin Song, Qiuling Wang, Yongfeng Yang, Zhenzhen Huang, Yongming Xu, Yufeng Fu, Meizhou Ding, Gang Fan, Jingnan Ren, Aiqun Yu, Jian Feng, Guijie Li, Linhua Huang, Yujiao Cheng

{"title":"A strategy of extracting and purifying the α-terpineol obtained from the Penicillium digitatum biotransformation of limonene.","authors":"Zehao Li, Zixuan Wang, Lulu Zhang, Feng Yingjie, Jinchu Yang, Haoliang Li, Weimin Song, Qiuling Wang, Yongfeng Yang, Zhenzhen Huang, Yongming Xu, Yufeng Fu, Meizhou Ding, Gang Fan, Jingnan Ren, Aiqun Yu, Jian Feng, Guijie Li, Linhua Huang, Yujiao Cheng","doi":"10.1080/10826068.2025.2494102","DOIUrl":"https://doi.org/10.1080/10826068.2025.2494102","url":null,"abstract":"α-Terpineol, an abundant oxygenated monoterpene compound, shows a varied range of beneficial bioactivities. This study focused on the efficient extraction and purification of α-terpineol from the biotransformation of limonene using Penicillium digitatum DSM 62840 mutant (PdTP1-overexpressed OE2 strain). The α-terpineol was primarily distributed in the supernatant, with minimal association with the biomass. And the optimal extraction was achieved using ethyl acetate as the extractant, which can directly obtain the highest recovery of α-terpineol without sequential extractions. The equilibrium of mass transfer was quickly reached (≤20 s in vortex). Additionally, the purification method of α-terpineol using column chromatography was described, further improving the purity of product to 96.86% in gas chromatography. Structural identification of purified α-terpineol was confirmed using gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance. This simplified and efficient strategy for the extraction and purification of α-terpineol not only provides a solid theoretical basis for the final step in the relevant study of microbial synthesis of α-terpineol but also is of great significance for its industrial application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, and application on inulin hydrolysis. 土曲霉URM4658菊粉酶在海藻酸钙微球中的固定化、生化特性和动力学/热力学参数的评价及其在菊粉水解中的应用

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-21 DOI: 10.1080/10826068.2025.2486424

Wallace Ribeiro da Silva, Camila Fernanda de Aquino Luna, Joyce Gueiros Wanderley Siqueira, Jorge Vinícius Fernandes Lima Cavalcanti, Rodrigo Lira de Oliveira, Tatiana Souza Porto

{"title":"Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, and application on inulin hydrolysis.","authors":"Wallace Ribeiro da Silva, Camila Fernanda de Aquino Luna, Joyce Gueiros Wanderley Siqueira, Jorge Vinícius Fernandes Lima Cavalcanti, Rodrigo Lira de Oliveira, Tatiana Souza Porto","doi":"10.1080/10826068.2025.2486424","DOIUrl":"https://doi.org/10.1080/10826068.2025.2486424","url":null,"abstract":"The present study aimed to immobilize an inulinase obtained from Aspergillus terreus URM4658 by entrapment in calcium alginate beads. The immobilization process yielded a satisfactory yield (92.72%) using 1.25% sodium alginate and 0.35 M CaCl2 with a curing time of 90 min. The immobilized enzyme exhibited optimum pH and temperature at 7.0 and 60 °C, respectively, showing an increased affinity for the substrate after the immobilization process, as evidenced by the decrease in Km compared to its free form. Moreover, the immobilized inulinase demonstrated good thermostability at 50 and 60 °C, as observed from the t1/2 (649.83-420.84 min) and D-values (2158.67-1398.00 min), respectively. The immobilized biocatalyst also exhibited good reusability, maintaining 92.73% of residual activity after 10 reaction cycles and no loss of activity after 30 days of storage. A continuous inulin hydrolysis operation in a packed bed reactor was performed using the immobilized inulinase, and a maximum release of total reducing sugars and nystose of 3.27 and 0.82 g L-1, respectively, was observed. The results indicate that an immobilized biocatalyst is a promising alternative for bioprocess involving inulin-rich feedstocks.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step rapid production of DNA polymerase from Pyrococcus furiosus in Escherichia coli system under optimized culture conditions. 优化培养条件下，在大肠杆菌体系中一步快速生产荧光焦球菌DNA聚合酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-17 DOI: 10.1080/10826068.2025.2483238

Fina Amreta Laksmi, Mulyorini Rahayuningsih, Arfena Rizqi Amalia, Wiga Alif Violando, Isa Nuryana, Yogi Ertanto, Yudhi Nugraha

{"title":"One-step rapid production of DNA polymerase from Pyrococcus furiosus in Escherichia coli system under optimized culture conditions.","authors":"Fina Amreta Laksmi, Mulyorini Rahayuningsih, Arfena Rizqi Amalia, Wiga Alif Violando, Isa Nuryana, Yogi Ertanto, Yudhi Nugraha","doi":"10.1080/10826068.2025.2483238","DOIUrl":"https://doi.org/10.1080/10826068.2025.2483238","url":null,"abstract":"The Pyrococcus furiosus (Pfu) DNA polymerase is an enzyme widely used in PCR due to its high fidelity, thermal stability, and ability to amplify even minute amounts of DNA with exceptional specificity and sensitivity. This study addresses the growing demand for efficient and cost-effective production of Pfu polymerase by optimizing its recombinant expression in Escherichia coli BL21 star (DE3) using a synthetic gene in the pD451-SR plasmid. Key parameters, including IPTG concentration (0.4 mM), post-induction incubation time (12 h), and cell density prior to induction (OD600 of 0.4), were systematically optimized to maximize yield and activity. A rapid and straightforward purification protocol was developed, utilizing a boiling-mediated lysis method that effectively purified Pfu polymerase within 20 minutes. A total of 795 mg/L of pure Pfu polymerase under optimized conditions, led to a 30-fold increase in protein yield with an enzyme activity of 2210 units, with SDS-PAGE and western blot analyses confirming a single protein band at 90 kDa. The optimized production and purification methods significantly enhance the time and cost-efficiency of Pfu polymerase production, making it highly suitable for industrial-scale applications. This streamlined approach positions Pfu polymerase as a valuable tool for routine PCR, cloning, mutagenesis, and advanced applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decayed fruit waste juice as a sustainable medium for high-yield microbial cellulose production: optimization, characterization and bioactivity studies. 作为高产微生物纤维素生产的可持续培养基的腐烂果渣汁：优化、表征和生物活性研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-09 DOI: 10.1080/10826068.2025.2489535

Sheetal Singh, Isha Santhosh, Subbalaxmi Selvaraj, Sowmya R Holla

{"title":"Decayed fruit waste juice as a sustainable medium for high-yield microbial cellulose production: optimization, characterization and bioactivity studies.","authors":"Sheetal Singh, Isha Santhosh, Subbalaxmi Selvaraj, Sowmya R Holla","doi":"10.1080/10826068.2025.2489535","DOIUrl":"https://doi.org/10.1080/10826068.2025.2489535","url":null,"abstract":"In the present study, microbial cellulose (MC) was produced from decayed fruit waste juice as a fermentation medium using a consortium of microbes grown on vegetable and fruit waste as the inoculum. To optimize the MC yield, the central composite design (CCD) of response surface methodology (RSM) was applied. Four factors at five different levels were chosen in the design with a total of 26 experimental runs obtained from the CCD design. The optimal conditions were fruit waste extract (60% v/v), glucose concentration (0.75% w/v), inoculum size (5% v/v), and fermentation time (5 d) which displayed a 2.1-fold increase in MC yield of 21.719 g/L. The MC was characterized using Fourier Transform Infrared Spectroscopy, X-ray Diffraction, Scanning Electron Microscopy, and Thermogravimetric; and compared its properties to cellulose produced through other techniques. The produced MC was examined for its antioxidant properties using cyclic voltammetry (CV) displaying an anodic peak at 1.15 V. Lastly, the antimicrobial activity was tested against four different microbial strains. Among them, MC exhibited a clear zone of 7.661 ± 0.256 cm2 against E. coli. The results of this study concluded that decayed fruit waste juice is a sustainable and economical fermentation medium.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freeze-thaw assisted immobilization of Rhodococcus erythropolis MTCC 3951 in kappa carrageenan for efficient terephthalic acid biodegradation. 冻融辅助固定红球菌 MTCC 3951 在卡帕卡拉胶中高效降解对苯二甲酸。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-07 DOI: 10.1080/10826068.2025.2487182

Ankita C Maurya, Amrik Bhattacharya, Sunil K Khare

{"title":"Freeze-thaw assisted immobilization of Rhodococcus erythropolis MTCC 3951 in kappa carrageenan for efficient terephthalic acid biodegradation.","authors":"Ankita C Maurya, Amrik Bhattacharya, Sunil K Khare","doi":"10.1080/10826068.2025.2487182","DOIUrl":"https://doi.org/10.1080/10826068.2025.2487182","url":null,"abstract":"Terephthalic acid (TPA) is widely used in the production of polyethylene terephthalate (PET) plastics and is one of PET's degraded products. However, TPA poses environmental health risks due to its persistence and potential toxicity, and thus, it is referred to as one of the major environmental pollutants. The present study describes the application of immobilized Rhodococcus erythropolis MTCC 3951 cells for TPA biodegradation to develop a viable bioprocess. Kappa carrageenan (KC) matrix was used to immobilize the cells. The entrapment conditions were modified for maximum TPA degradation and minimum cell leakage. The entrapped cells were cryofreeze at -20 °C for 24 h, followed by thawing at 24 °C for 4 h. Cryofreezing was introduced to enhance the cell's stability and mechanical strength, required for effective reusability. The stability of the matrix was confirmed by FTIR, TGA, and FESEM. The cryofreeze entrapped cells completely degrade 5 mM TPA within 14 h of treatment. The immobilized cells could be reused for at least 10 repeated cycles without loss in degradation efficiency. The cryofreezing of immobilized cells in KC is a novel technique to enhance the system's stability. The developed bioprocess could be used efficiently to alleviate TPA pollution from the environment.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture. 传统培养基和工业废水培养基对蓟马培养过程中生物量生产、营养动态和脂肪酸组成的影响。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-04 DOI: 10.1080/10826068.2025.2484606

Pedro Contreras, Claudia Oviedo, Robinson Soto-Ramírez, Cinthia Vásquez-Sandoval, José Navarrete, Patricio Dantagnan

{"title":"Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture.","authors":"Pedro Contreras, Claudia Oviedo, Robinson Soto-Ramírez, Cinthia Vásquez-Sandoval, José Navarrete, Patricio Dantagnan","doi":"10.1080/10826068.2025.2484606","DOIUrl":"https://doi.org/10.1080/10826068.2025.2484606","url":null,"abstract":"The rising demand for polyunsaturated fatty acids, coupled with the decline of traditional fish-based sources, highlights Ulkenia visurgensis Lng2, a newly isolated thraustochytrid strain, as a promising alternative for sustainable polyunsaturated fatty acids production. Using Response Surface Methodology to optimize medium composition and culture conditions, biomass production was improved to 11.64 g/L-120% higher than previous studies-with 35% of the biomass consisting of total lipids. Of the total fatty acids, 40% corresponded to polyunsaturated fatty acids, including 27% of docosahexaenoic acid, 4% of eicosapentaenoic acid, and 7% of arachidonic acid. Alternative media, such as wastewaters from corn and fish meal processing, were also evaluated. Biomass yields in wastewaters-based media (30% v/v) were lower due to limited nutrient availability, achieving between 2.96 g/L and 2.28 g/L, with the strain showing mostly around 8% carbon and nitrogen assimilation. Morphological changes, including increased vegetative and multinucleated cells, were also observed. Lipid content decreased in wastewater-based cultures, yet PUFAs constituted 47% of total fatty acids, with EPA nearly doubling, DHA remaining stable and decreased ARA content. These results shed light on how U. visurgensis Lng2 adapts to nutrient-limited environments and offer valuable insights for developing sustainable PUFA production using industrial by-products as alternative media.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of different light spectra on selenium bioaccumulation by Spirulina platensis cyanobacteria in flat plate photobioreactors. 平板光生物反应器中不同光谱对蓝藻硒生物累积的影响

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-11 DOI: 10.1080/10826068.2024.2426744

Yeganeh Shirazi, Salar Helchi, Mir Mehrshad Emamshoushtari, Sina Niakan, Elnaz Sohani, Farshid Pajoum Shariati

{"title":"The effect of different light spectra on selenium bioaccumulation by Spirulina platensis cyanobacteria in flat plate photobioreactors.","authors":"Yeganeh Shirazi, Salar Helchi, Mir Mehrshad Emamshoushtari, Sina Niakan, Elnaz Sohani, Farshid Pajoum Shariati","doi":"10.1080/10826068.2024.2426744","DOIUrl":"10.1080/10826068.2024.2426744","url":null,"abstract":"Selenium (Se) plays a crucial role in human health, influencing conditions such as cancer, diabetes, and neurological disorders. With global population growth and unequal nutrient distribution threatening food security, new approaches are needed to meet the nutritional needs of the world. Se is essential for immune function, metabolism, and antioxidant defense, and in regions suffering from food insecurity and malnutrition, selenium-enriched food could offer an affordable solution. Spirulina platensis, microalgae, can bioaccumulate Se from its environment, enhancing its nutritional value. This study explores how different light spectra (red, white, yellow, and blue LEDs) affect Se bioaccumulation in Spirulina when Na2SeO3 is added to the culture medium in photobioreactors. The results show that red light made the highest Se bioaccumulation (0.118 mg.L-1), followed by white, yellow, and blue light. Se addition also increased cell dry weight by 46%, 33%, 22%, and 60%, respectively, compared to photobioreactors without Se, with biomass productivity highest under red light. Furthermore, Se boosted maximum Chl α concentration, improving photosynthetic efficiency. These findings suggest that optimizing light conditions can significantly enhance the nutritional value of Spirulina, offering a potential solution to global hunger by providing a sustainable, selenium-enriched food source.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"491-501"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered production of 5-aminolevulinic acid in recombinant Escherichia coli BL21. 在重组大肠杆菌 BL21 中设计生产 5-氨基乙酰丙酸。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-11-05 DOI: 10.1080/10826068.2024.2423644

Zhiwen Zhu, Bing Fu, Jiajie Lu, Peize Wang, Chuyang Yan, Fuyao Guan, Jianying Huang, Ping Yu

{"title":"Engineered production of 5-aminolevulinic acid in recombinant Escherichia coli BL21.","authors":"Zhiwen Zhu, Bing Fu, Jiajie Lu, Peize Wang, Chuyang Yan, Fuyao Guan, Jianying Huang, Ping Yu","doi":"10.1080/10826068.2024.2423644","DOIUrl":"10.1080/10826068.2024.2423644","url":null,"abstract":"5-aminolevulinic acid (ALA) is a non-protein amino acid that has been widely used in the fields of medicine and agriculture. This study aims to engineer the C5 pathway of the ALA biosynthesis in Escherichia coli BL21 to enhance ALA production. The ALA synthase genes gltX, hemA, and hemL were overexpressed in E. coli BL21 to lead to the increase in the production of ALA. The sRNA RyhB was also overexpressed to downregulate the expression of ALA dehydratase to reduce the downstream bioconversion of ALA to porphobilinogen. Next, the gene arcA was knocked out by CRISPR-Cas9 technology to open the TCA cycle to promote the respiratory metabolism of the strain to reduce the feedback inhibition of heme to ALA. The fermentation conditions of the engineered strain were optimized by response surface experiments. The time-course analysis of the ALA production was carried out in a 1 L shake flask. Through these efforts, the production of ALA in engineered strain reached 2953 mg/L in a 1 L shake flask. This study contributes to the industrial production of ALA by the engineered E. coli in the future.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"446-456"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in growth and biosynthetic activity of Calendula officinalis hairy roots. 金盏花毛根的生长和生物合成活性的变化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-10-21 DOI: 10.1080/10826068.2024.2418015

Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk

{"title":"Variability in growth and biosynthetic activity of Calendula officinalis hairy roots.","authors":"Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk","doi":"10.1080/10826068.2024.2418015","DOIUrl":"10.1080/10826068.2024.2418015","url":null,"abstract":"Calendula officinalis is a widespread medicinal plant with a sufficiently well-studied chemical composition. Secondary metabolites synthesized by C.officinalis plants have pharmacological value for treating numerous diseases, and various types of aseptic in vitro cultures can be used as a source of these compounds. From this perspective, hairy roots attract considerable attention for the production of bioactive chemicals, including flavonoids with antioxidant activity. This paper shows the possibility of C.officinalis hairy roots obtaining with 100% frequency by Agrobacterium rhizogenes genetic transformation. Hairy root lines differed in growth rate and flavonoid content. In particular, flavonoids were accumulated in the amount of up to 6.68 ± 0.28 mg/g of wet weight. Methyl jasmonate in the concentration of 10 µM inhibited root growth to a small extent but stimulated the synthesis of flavonoids. The antioxidant activity and the reducing power increased in the roots grown in the medium with methyl jasmonate. The strong correlation of antioxidant activity and reducing power with flavonoid content was detected. The influence of extraction conditions on the content of flavonoids in the extracts and their bioactivity was determined. The potent reducing activity of extracts from hairy roots allowed the production of silver nanoparticles, which was confirmed by transmission electron microscopy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"381-391"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0