Preparative Biochemistry & Biotechnology最新文献_第2页

Biochemical characterization, stability, and kinetics of three substrates of the recombinant TMPRSS2 serine protease domain. 重组 TMPRSS2 丝氨酸蛋白酶结构域三种底物的生化特性、稳定性和动力学。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-10 DOI: 10.1080/10826068.2024.2349132

Flávio Antônio de Oliveira-Simões, Isabela Victorino da Silva Amatto, Camila Langer Marciano, Nathalia Gonsales da Rosa-Garzon, Débora Noma Okamoto, Maria Aparecida Juliano, Luiz Juliano, Hamilton Cabral

{"title":"Biochemical characterization, stability, and kinetics of three substrates of the recombinant TMPRSS2 serine protease domain.","authors":"Flávio Antônio de Oliveira-Simões, Isabela Victorino da Silva Amatto, Camila Langer Marciano, Nathalia Gonsales da Rosa-Garzon, Débora Noma Okamoto, Maria Aparecida Juliano, Luiz Juliano, Hamilton Cabral","doi":"10.1080/10826068.2024.2349132","DOIUrl":"10.1080/10826068.2024.2349132","url":null,"abstract":"Transmembrane serine protease 2 (TMPRSS2) is a membrane-bound protease belonging to the type II transmembrane serine protease (TTSP) family. It is a multidomain protein, including a serine protease domain responsible for its self-activation. The protein has been implicated as an oncogenic transcription factor and for its ability to cleave (prime) the SARS-CoV-2 spike protein. In order to characterize the TMPRSS2 biochemical properties, we expressed the serine protease domain (rTMPRSS2_SP) in Komagataella phaffii using the pPICZαA vector and purified it using immobilized metal affinity (Ni Sepharose™ excel) and size exclusion (Superdex 75) chromatography. We explored operational fluorescence resonance energy transfer FRET peptides as substrates. We chose the peptide Abz-QARK-(Dnp)-NH2 (Abz = ortho-aminobenzoic acid, the fluorescence donor, and Dnp = 2,4-dinitrophenyl, the quencher group) as a substrate to find the optimal conditions for maximum enzymatic activity. We found that metallic ions such as Ca2+ and Na+ increased enzymatic activity, but ionic surfactants and reducing agents decreased catalytic capacity. Finally, we determined the rTMPRSS2_SP stability for long-term storage. Altogether, our results represent the first comprehensive characterization of TMPRSS2's biochemical properties, providing valuable insights into its serine protease domain.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1285-1293"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures. 在热诱导大肠杆菌分批进行喂养培养的重组 HuGM-CSF 生产缩减模型中对葡萄糖和氧气梯度的综合研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-03 DOI: 10.1080/10826068.2024.2347403

Greta I Reynoso-Cereceda, Norma A Valdez-Cruz, Nestor O Pérez, Mauricio A Trujillo-Roldán

{"title":"A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures.","authors":"Greta I Reynoso-Cereceda, Norma A Valdez-Cruz, Nestor O Pérez, Mauricio A Trujillo-Roldán","doi":"10.1080/10826068.2024.2347403","DOIUrl":"10.1080/10826068.2024.2347403","url":null,"abstract":"The effect of gradients of elevated glucose and low dissolved oxygen in the addition zone of fed-batch E. coli thermoinduced recombinant high cell density cultures can be evaluated through two-compartment scale-down models. Here, glucose was fed in the inlet of a plug flow bioreactor (PFB) connected to a stirred tank bioreactor (STB). E. coli cells diminished growth from 48.2 ± 2.2 g/L in the stage of RP production if compared to control (STB) with STB-PFB experiments, when residence time inside the PFB was 25 s (34.1 ± 3.5 g/L) and 40 s (25.6 ± 5.1 g/L), respectively. The recombinant granulocyte-macrophage colony-stimulating factor (rHuGM-CSF) production decreased from 34 ± 7% of RP in inclusion bodies (IB) in control cultures to 21 ± 8%, and 7 ± 4% during the thermoinduction production phase when increasing residence time inside the PFB to 25 s and 40 s, respectively. This, along with the accumulation of acetic and formic acid (up to 4 g/L), indicates metabolic redirection of central carbon routes through metabolic flow and mixed acid fermentation. Special care must be taken when producing a recombinant protein in heat-induced E. coli, because the yield and productivity of the protein decreases as the size of the bioreactors increases, especially if they are carried at high cell density.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1263-1274"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, characterization and optimization of oleaginous Providencia vermicola as a feedstock for biodiesel production using Response Surface Methodology. 利用响应面方法对作为生物柴油生产原料的含油蛭藻进行分离、表征和优化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-10 DOI: 10.1080/10826068.2024.2344516

Temitope Abiola, Olumide D Olukanni

{"title":"Isolation, characterization and optimization of oleaginous Providencia vermicola as a feedstock for biodiesel production using Response Surface Methodology.","authors":"Temitope Abiola, Olumide D Olukanni","doi":"10.1080/10826068.2024.2344516","DOIUrl":"10.1080/10826068.2024.2344516","url":null,"abstract":"Oleaginous organisms accrue more than twenty percent of their biomass as lipids and hence are promising feedstocks for biodiesel production. In this study, lipid accumulating bacteria were isolated from diesel-contaminated soils and screened with Sudan black B stain. The most oleaginous was done using 16s rRNA gene sequencing. Lipid production was initially optimized based on media, nitrogen source, pH and temperature. Response surface methodology (RSM) was then employed for the enhancement of lipid weight and content. Obtained lipid was converted to biodiesel using direct transesterification, and both lipid and biodiesel were characterized using FTIR. A total of thirteen bacteria were isolated and the most prominent lipid producer was identified as Providencia vermicola with lab number BA6. Preliminary optimization studies revealed optimum lipid production when nutrient broth and acetic acid served as carbon source; KNO3 as nitrogen source, pH 7.0 and 30 °C. Optimization using RSM resulted in a 5.1% and 74.1% increase in the biomass and lipid content of BA6 respectively. FTIR analyses confirmed the presence of functional groups characteristic of lipids and biodiesel. P. vermicola is a novel oleaginous organism that represents a promising feedstock for biodiesel production.HIGHLIGHTSThe bacterium designated as BA6 identified as Providencia vermicola has the highest lipid contents of the oleaginous bacteria isolated.It accumulates lipids up to 47.73 % of its biomassThe percentage lipids accumulation increased to about 74 % when RSM was used.Providencia vermicola is being reported as an oleaginous organism for the first time.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1226-1242"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resin screening and process optimization for erythritol mother liquor chromatographic separation. 用于赤藓糖醇母液色谱分离的树脂筛选和工艺优化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-14 DOI: 10.1080/10826068.2024.2349936

Haiyang Li, Xiangying Zhao, Liping Liu, Mingjing Yao, Yanlei Han, Ruiguo Li, Jianjun Liu, Jiaxiang Zhang

{"title":"Resin screening and process optimization for erythritol mother liquor chromatographic separation.","authors":"Haiyang Li, Xiangying Zhao, Liping Liu, Mingjing Yao, Yanlei Han, Ruiguo Li, Jianjun Liu, Jiaxiang Zhang","doi":"10.1080/10826068.2024.2349936","DOIUrl":"10.1080/10826068.2024.2349936","url":null,"abstract":"In order to improve the utilization value of the erythritol mother liquor, the separation and purification of the erythritol mother liquor was selected in this study. The selected chromatographic separation programme for erythritol crystallizing mother liquor is as follows: Firstly, erythritol is resolved from mannitol and arabitol with DTF-01Ca (Suqing Group) resin and then mannitol is resolved from arabitol with 99Ca/320 (Dowex) resin. At the same time, the chromatographic conditions of the DTF-01Ca (Suqing Group) and 99Ca/320 (Dowex) resins were optimized, resulting in an optimal separation temperature and mobile phase flow rate of 70 °C, 10 ml/min. On this basis, a single-column chromatographic model was used to calculate the TD model parameter (<math><mrow><mi>N</mi></mrow></math>) and the mass transfer coefficient (<math><msub><mrow><mi>k</mi></mrow><mrow><mi>m</mi><mi> </mi></mrow></msub></math>) of the separation of erythritol mother liquor by DTF-01Ca (Suqing Group) and 99Ca/320 (Dowex) resins. The adsorption isotherms, TD model parameter (<math><mrow><mi>N</mi></mrow></math>) and the mass transfer coefficient (<math><msub><mrow><mi>k</mi></mrow><mrow><mi>m</mi><mi> </mi></mrow></msub></math>) provides data references for the design and operation of the simulated moving beds (SMB) separation system for the industrial-scale separation of erythritol crystallizing mother liquor.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1294-1305"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of low-grade walnut kernels for oil extraction using eco-friendly methods: a comparative analysis of oil composition, antioxidant and antimicrobial activity. 利用生态友好型方法提取低级核桃仁油：油成分、抗氧化剂和抗菌活性的比较分析。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-07-16 DOI: 10.1080/10826068.2024.2345244

Iqra Mohiuddin Bhat, Shoib Mohmad Wani, Sajad Ahmad Mir, Farooq A Masoodi, Saiqa Bhat

{"title":"Utilization of low-grade walnut kernels for oil extraction using eco-friendly methods: a comparative analysis of oil composition, antioxidant and antimicrobial activity.","authors":"Iqra Mohiuddin Bhat, Shoib Mohmad Wani, Sajad Ahmad Mir, Farooq A Masoodi, Saiqa Bhat","doi":"10.1080/10826068.2024.2345244","DOIUrl":"10.1080/10826068.2024.2345244","url":null,"abstract":"Walnut oil was extracted using three different eco-friendly extraction methods, solvent extraction (using ethyl acetate [EA] and ethanol [ET]), aqueous enzymatic extraction (AEE), and ultrasound-assisted enzymatic extraction (UAEE), and their lipid yield, lipid composition, physicochemical analysis, mineral composition, total phenols, antioxidant capacity, and antimicrobial activity were analyzed and compared. The AEE technique offered a greater yield (50.6%) than the other extraction methods and gave comparatively higher linoleic acid (66.12%) content. Palmitic, oleic, linoleic, linolenic, and stearic acids were the principal components that GC/MS detected in all the oil samples. UAEE produced the most polyphenols (0.49 mgGAE/g), followed by AEE (0.46 mgGAE/g), EA (0.45 mgGAE/g), and ET (0.35 mgGAE/g). The DPPH assay results were in the order of UAEE (191 μmolTE/kg) > AEE (186 μmolTE/kg) > EA (153 μmolTE/kg) > ET (130 μmolTE/kg). The FRAP assay findings showed a similar pattern: UAEE (112 molTE/kg) > AEE (102 molTE/kg) > EA (96 molTE/kg) > ET (82 molTE/kg). Results suggested that for a higher extraction yield, AEE is the better technique and UAEE is the recommended method for enhancing walnut oil antioxidant capacity. Additionally, it was found that polyphenols considerably increased the antioxidant capacity of walnut oil and are thought to be health-promoting. The results demonstrated the antibacterial effectiveness of the extracted oil against Bacillus subtilis, Bacillus licheniformis, and Staphylococcus aureus. This study provides information about low-cost and ecofriendly technologies of walnut oil extraction for food, cosmetic, and medical uses.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1243-1252"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of esculin acetates through transesterification reaction catalyzed by Novozyme 435^® and their Purification followed by NMR characterization. 在 Novozyme 435® 催化下通过酯交换反应制备乙酸酯，并对其进行纯化和核磁共振表征。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-31 DOI: 10.1080/10826068.2024.2415961

Maryna Schuenck Knupp, Camila Rodrigues Adão Malafaia, Patrícia Homobono Brito de Moura, Denise Maria Guimarães Freire, Luzineide Wanderley Tinoco, Shaft Corrêa Pinto, Michelle Frazão Muzitano, Ivana Correa Ramos Leal

{"title":"Preparation of esculin acetates through transesterification reaction catalyzed by Novozyme 435® and their Purification followed by NMR characterization.","authors":"Maryna Schuenck Knupp, Camila Rodrigues Adão Malafaia, Patrícia Homobono Brito de Moura, Denise Maria Guimarães Freire, Luzineide Wanderley Tinoco, Shaft Corrêa Pinto, Michelle Frazão Muzitano, Ivana Correa Ramos Leal","doi":"10.1080/10826068.2024.2415961","DOIUrl":"https://doi.org/10.1080/10826068.2024.2415961","url":null,"abstract":"In this study, biocatalytic transesterification reaction using Novozyme 435® (N435) lipase was employed to enhance the hydrophobicity of esculin, aiming to improve its solubility for commercial applications and enhance its bioactivity and oral viability. The acylation reaction of esculin with vinyl acetate was conducted at 60 °C and 200 rpm for 24 h. After chromatographic and spectroscopic analysis, two products were identified: the first one was monoacylated at the 6'-OH position of the glucosyl moiety of esculin (TR: 10.3 min and m/z 382.93 [M + H]+), and the second one was diacylated at the 6'-OH and 3'-OH positions (TR: 13.0 min and m/z 424.93 [M + H]+). The latter was the major product, with a conversion rate of 53.550 ± 0.368%, while the monoacetylated one showed 8.715 ± 0.064%. Both products were isolated by high-speed counter-current chromatography (HSCCC) using a two-phase system HEMWat 1:9:1:9 and characterized by NMR. In this way, these results improve the practical application of esculin, through the obtention of esculin mono and diacetates by fast and efficient biocatalysis reaction.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide. 不同提取方法对条纹叶紫苏多糖结构和抗氧化性的影响

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-30 DOI: 10.1080/10826068.2024.2419862

Xue Han, Hai Liu, Yeshan Zhang, Yi Zhang, Zhiqin Song, Lili Yang, Xiao Liu, Lin Yang, Mingkai Wu, Longyan Tan

{"title":"The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide.","authors":"Xue Han, Hai Liu, Yeshan Zhang, Yi Zhang, Zhiqin Song, Lili Yang, Xiao Liu, Lin Yang, Mingkai Wu, Longyan Tan","doi":"10.1080/10826068.2024.2419862","DOIUrl":"https://doi.org/10.1080/10826068.2024.2419862","url":null,"abstract":"Bletilla striata polysaccharide (BSP) is one of the main active ingredients of the traditional Chinese medicine Bletilla striata (Thunb) Richb.f and the extraction method of BSP has a significant impact on its properties. This study investigated the effects of four extraction methods, namely hot water extraction, ultrasonic extraction, enzyme extraction, and microwave extraction, on the structure and antioxidant properties of BSP. Characterization results from FTIR and NMR showed that all four BSP consisted mainly of glucose and mannose, forming α-glycosidic and β-glycosidic linkages to form glucomannan. Hot water extraction had the lowest extraction rate of BSP at 21.78% ± 0.73%. The polysaccharide BSP-H obtained from hot water extraction had the smallest absolute Zeta potential and Grain size, but the largest molecular weight at 204 kDa. It exhibited the best thermal stability and superior antioxidant activity compared to polysaccharides extracted using the other three methods, as evaluated by three different antioxidant assays. Although the antioxidant activity of BSP-V was slightly weaker, it showed a significant improvement compared to the remaining two polysaccharides. These results suggest that hot water extraction is the most suitable method for large-scale application of BSP, preserving its activity effectively, thus facilitating practical production and product development.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated formic acid and deep eutectic solvent mediated sustainable synthesis of cellulose nanocrystals from Sterculia foetida shells. 以甲酸和深共晶溶剂为介质，从 Sterculia foetida 贝壳中持续合成纤维素纳米晶体。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-30 DOI: 10.1080/10826068.2024.2419873

Kurappalli Rohil Kumar, Nirajha Vishnu, Gnanabarathi C S, Kiran Babu Uppuluri, Rangabhashiyam Selvasembian

{"title":"Integrated formic acid and deep eutectic solvent mediated sustainable synthesis of cellulose nanocrystals from Sterculia foetida shells.","authors":"Kurappalli Rohil Kumar, Nirajha Vishnu, Gnanabarathi C S, Kiran Babu Uppuluri, Rangabhashiyam Selvasembian","doi":"10.1080/10826068.2024.2419873","DOIUrl":"https://doi.org/10.1080/10826068.2024.2419873","url":null,"abstract":"The present study reports the green synthesis of cellulose nanocrystals from the shells of Sterculia foetida (SFS) cellulose. Three different methods, alkali, acid and organic acid, were screened for the maximum cellulose extraction. A maximum cellulose yield, 30.6 ± 0.84 w/w, was obtained using 90% formic acid at 110 °C in 120 min. The extracted cellulose was characterized and identified by instrumental analyses. SEM analysis showed skeletal rod-like microfibril structures and similar intra-fibrillar widths. CP/MAS 13C NMR and FTIR spectrum revealed the purity of cellulose and the absence of other components like hemicellulose and lignin. XRD study revealed a cellulose crystallinity index of 88.07%. BET analysis showed a good surface area (3.3213 m2/g) and a micro-pore area of 1.871 m2/g. The cellulose nanocrystals were synthesized from the extracted cellulose using deep eutectic solvents (DES), choline chloride and lactic acid (1:2 ratio). The cellulose nanocrystals (CNC) synthesized from DES-based exhibited zeta potential and particle size of -16.7 mV and 576.3 d.nm. DES-synthesized cellulose nanocrystals were spherical-like shapes, as observed from TEM images. The present results exposed that formic acid is an effective and green catalyst for the extraction of cellulose and DES for the sustainable synthesis of CNC.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in growth and biosynthetic activity of Calendula officinalis hairy roots. 金盏花毛根的生长和生物合成活性的变化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-21 DOI: 10.1080/10826068.2024.2418015

Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk

{"title":"Variability in growth and biosynthetic activity of Calendula officinalis hairy roots.","authors":"Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk","doi":"10.1080/10826068.2024.2418015","DOIUrl":"https://doi.org/10.1080/10826068.2024.2418015","url":null,"abstract":"Calendula officinalis is a widespread medicinal plant with a sufficiently well-studied chemical composition. Secondary metabolites synthesized by C.officinalis plants have pharmacological value for treating numerous diseases, and various types of aseptic in vitro cultures can be used as a source of these compounds. From this perspective, hairy roots attract considerable attention for the production of bioactive chemicals, including flavonoids with antioxidant activity. This paper shows the possibility of C.officinalis hairy roots obtaining with 100% frequency by Agrobacterium rhizogenes genetic transformation. Hairy root lines differed in growth rate and flavonoid content. In particular, flavonoids were accumulated in the amount of up to 6.68 ± 0.28 mg/g of wet weight. Methyl jasmonate in the concentration of 10 µM inhibited root growth to a small extent but stimulated the synthesis of flavonoids. The antioxidant activity and the reducing power increased in the roots grown in the medium with methyl jasmonate. The strong correlation of antioxidant activity and reducing power with flavonoid content was detected. The influence of extraction conditions on the content of flavonoids in the extracts and their bioactivity was determined. The potent reducing activity of extracts from hairy roots allowed the production of silver nanoparticles, which was confirmed by transmission electron microscopy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review. 从天然资源中提取类胡萝卜素的绿色和可持续技术：综述。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-20 DOI: 10.1080/10826068.2024.2402905

Surbhi Sharma, Anshika Dedha, Manju M Gupta, Nahar Singh, Arvind Gautam, Abha Kumari

{"title":"Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review.","authors":"Surbhi Sharma, Anshika Dedha, Manju M Gupta, Nahar Singh, Arvind Gautam, Abha Kumari","doi":"10.1080/10826068.2024.2402905","DOIUrl":"https://doi.org/10.1080/10826068.2024.2402905","url":null,"abstract":"In recent years, driven by increasing consumer demand for natural and healthy convenient foods, the food industry has been shifting from synthetic to natural products. This shift is also reflected in the growing popularity of non-conventional extraction methods for pigments, which are favored for sustainability and environment-friendliness compared to conventional processes. This review aims to investigate the extraction of carotenoids from a variety of natural sources, including marine sources like fungus, microalgae, and crustaceans, as well as widely studied plants like tomatoes and carrots. Additionally, it delves into the recovery of valuable carotenoids from waste products like pomace and peels, highlighting the nutritional and environmental benefits. The review also emphasizes the role of green solvents such limonene, vegetable oils, ionic liquids, supercritical fluids, and natural deep eutectic solvents in effective and ecologically friendly carotenoid extraction. These technologies support the ideas of a circular and sustainable economy in addition to having a smaller negative impact on the environment. Overall, the present study highlights the crucial importance of green extraction technologies in achieving the dual goals of sustainability and public safety.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-33"},"PeriodicalIF":2.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0