Preparative Biochemistry & Biotechnology最新文献_第10页

Efficient RNA extraction method for acquiring high-quality RNA from various tissues of the fiber crop abaca, Musa textilis Née. 用于从纤维作物巴西蕉（Musa textilis Née）的各种组织中获取高质量 RNA 的高效 RNA 提取方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-12-17 DOI: 10.1080/10826068.2024.2440421

Rhosener Bhea L Koh, Jose Planta, Richard I Encarnacion, Jasca Gayle G Española, Vermando M Aquino, Leny C Galvez

{"title":"Efficient RNA extraction method for acquiring high-quality RNA from various tissues of the fiber crop abaca, Musa textilis Née.","authors":"Rhosener Bhea L Koh, Jose Planta, Richard I Encarnacion, Jasca Gayle G Española, Vermando M Aquino, Leny C Galvez","doi":"10.1080/10826068.2024.2440421","DOIUrl":"10.1080/10826068.2024.2440421","url":null,"abstract":"Isolation of high-quality RNA from abaca is very challenging due to the presence of polyphenols, polysaccharides, and its high fiber content. In this study, we compared six extraction methods across three tissue types and different developmental stages (in-vitro-grown young versus field-grown mature tissue). The Invitrogen PureLink RNA kit proved to be the most efficient in extracting RNA from young abaca tissues (leaves, pseudostem, and corm). The quality of RNA extracted from young tissues was further assessed by RNA-seq applications, with raw sequencing reads mapping back to the M. textilis reference genome at rates of 86.0%-90.4%. The SDS-TRIzol-method modified with an added on-column DNAse I treatment was used to extract RNA from mature tissues (leaves, midrib, and pseudostem). RNA isolated from five M. textilis cultivars and across three mature tissue types showed RNA yield per 100 mg of fresh weight ranges from 0.57 to 10.94 µg and RNA integrity number (RIN) scores of more than 7.0 for all tissue types. Our improved SDS-Trizol method for RNA extraction described here is simple and yields good quality RNAs from mature abaca tissues while the PureLink RNA kit is suitable for extracting RNA from young abaca samples amenable to RT-qPCR and next-generation sequencing studies.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"555-566"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides. 杜仲多糖超声辅助深度共熔溶剂提取工艺优化、表征及生物活性研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-02-01 DOI: 10.1080/10826068.2024.2441914

Yao Wen, Xiangyi Yan, Haiyun Chen

{"title":"Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides.","authors":"Yao Wen, Xiangyi Yan, Haiyun Chen","doi":"10.1080/10826068.2024.2441914","DOIUrl":"10.1080/10826068.2024.2441914","url":null,"abstract":"For the valorization of Eucommia ulmoides (EU) for the functional food industry, the process of ultrasonic-assisted deep eutectic solvent (DES) extraction of EU polysaccharides (EUP) was optimized by response surface methodology. After response surface analysis and experimental verification, the optimum extraction conditions were as follows: the molar ratio of choline chloride to oxalic acid was 0.9:1, the water content of DES was 40.28%, the ratio of material to liquid was 1:22 g/mL, the time was 90 min, and the power was 320 W. The extraction rate of EUP was 1.71%, which was higher than the extraction rate of 0.75% by ultrasonic water extraction under the same conditions, and the optimization effect was better. After DEAE column gradient elution and Sephadex G-75 gel column chromatography, the elution curve and a refined polysaccharide (EUP-1) were obtained. The IC50 values of EUP-1 against α-glucosidase and α-amylase were 0.091 and 0.011 mg/mL. The IC50 values of EUP-1 on DPPH and ABTS·+ scavenging were 0.065 and 0.065 mg/mL. The monosaccharide composition of EUP-1 was analyzed as mannose, rhamnose, glucuronic acid, and galacturonic acid in the following molar ratio: 1.00: 1.28: 1.53: 1.88.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"577-589"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Escherichia coli to metabolize sorbitol as the sole carbon source for synthesis of recombinant L-Asparaginase-II. 改造大肠杆菌，使其代谢山梨醇作为合成重组 L-天冬酰胺酶-II的唯一碳源。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-12-13 DOI: 10.1080/10826068.2024.2440425

Dibya Ranjan Das, Shubhashree Mahalik

{"title":"Engineering Escherichia coli to metabolize sorbitol as the sole carbon source for synthesis of recombinant L-Asparaginase-II.","authors":"Dibya Ranjan Das, Shubhashree Mahalik","doi":"10.1080/10826068.2024.2440425","DOIUrl":"10.1080/10826068.2024.2440425","url":null,"abstract":"Sorbitol, known as D-Glucitol, is a hexose sugar alcohol that occurs naturally in various fruits, including berries, cherries, plums, pears, and apples. It is noteworthy that sorbitol can be metabolized by microbes, plants, and humans through distinct pathways. Nevertheless, in bacteria like Escherichia coli (E. coli), sorbitol is not the primary carbon source and its utilization is generally suppressed due to carbon catabolite repression. In this context, Escherichia coli has been engineered to enable the use of sorbitol as the sole carbon source for producing recombinant proteins. This modification involves a two-plasmid system where the sorbitol-6-phosphate dehydrogenase (srlD) gene is upregulated under an araBAD promoter, while the recombinant protein is expressed from a second plasmid under the tac promoter. The overexpression of srlD in the engineered E. coli strain enhances the utilization of sorbitol as the sole carbon source. When cultured in a medium supplemented solely with sorbitol, the engineered E. coli strain exhibits a 3.6 times higher specific growth rate and yields substantially higher concentration of recombinant protein compared to the wild-type strain. Additionally, the engineered strain demonstrates a higher YP/X ratio than the wild-type strain.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"567-576"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteases, a powerful biochemical tool in the service of medicine, clinical and pharmaceutical. 蛋白酶是为医学、临床和制药服务的强大生化工具。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-22 DOI: 10.1080/10826068.2024.2364234

Ghadir A Jamal, Ehsan Jahangirian, Michael R Hamblin, Hamed Mirzaei, Hossein Tarrahimofrad, Neda Alikowsarzadeh

{"title":"Proteases, a powerful biochemical tool in the service of medicine, clinical and pharmaceutical.","authors":"Ghadir A Jamal, Ehsan Jahangirian, Michael R Hamblin, Hamed Mirzaei, Hossein Tarrahimofrad, Neda Alikowsarzadeh","doi":"10.1080/10826068.2024.2364234","DOIUrl":"10.1080/10826068.2024.2364234","url":null,"abstract":"Proteases, enzymes that hydrolyze peptide bonds, have various applications in medicine, clinical applications, and pharmaceutical development. They are used in cancer treatment, wound debridement, contact lens cleaning, prion degradation, biofilm removal, and fibrinolytic agents. Proteases are also crucial in cardiovascular disease treatment, emphasizing the need for safe, affordable, and effective fibrinolytic drugs. Proteolytic enzymes and protease biosensors are increasingly used in diagnostic and therapeutic applications. Advanced technologies, such as nanomaterials-based sensors, are being developed to enhance the sensitivity, specificity, and versatility of protease biosensors. These biosensors are becoming effective tools for disease detection due to their precision and rapidity. They can detect extracellular and intracellular proteases, as well as fluorescence-based methods for real-time and label-free detection of virus-related proteases. The active utilization of proteolytic enzymatic biosensors is expected to expand significantly in biomedical research, in-vitro model systems, and drug development. We focused on journal articles and books published in English between 1982 and 2024 for this study.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-25"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of fermentation on the structures of yellow compounds in citrus pomace. 发酵对柑橘渣中黄色化合物结构的影响。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-10 DOI: 10.1080/10826068.2024.2362794

Dan-Dan Yang, Wen-Jie Li, Sheng-Jiao Lei, Hai-Yan Liu, Nong-Fei Ouyang, Jun-Dong Zhu

引用次数: 0

Harnessing algal biomass for sustainable energy: cultivation, strain improvement, and biofuel production. 利用藻类生物质实现可持续能源：栽培、菌种改良和生物燃料生产。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-12-16 DOI: 10.1080/10826068.2024.2434879

Indira Mikkili, Bala Venkata Sai Teja Gaddirala, Sudarsini Borugadda, Syam Babu Davuluri

{"title":"Harnessing algal biomass for sustainable energy: cultivation, strain improvement, and biofuel production.","authors":"Indira Mikkili, Bala Venkata Sai Teja Gaddirala, Sudarsini Borugadda, Syam Babu Davuluri","doi":"10.1080/10826068.2024.2434879","DOIUrl":"10.1080/10826068.2024.2434879","url":null,"abstract":"The world faces pressing environmental challenges, including greenhouse gas emissions, global warming, climate change, and rising sea levels. Alongside, these issues, the depletion of fossil fuels has intensified the search for alternative energy sources. Algal biomass presents a promising long-term solution to these global problems. The quest for sustainable energy has driven significant research into algal biofuels as a viable alternative to fossil fuels. Algae offers several advantages as a feedstock for biofuel production, including high biomass yield, rapid growth rates, cost-effective cultivation, carbon dioxide fixation capabilities, and the potential to grow on non-arable land using non-potable water. This manuscript provides an overview of algal biomass cultivation using renewable feedstocks, identifies potential algal strains for biofuel production, and explores bioengineering advancements in algae. Additionally, strain improvement strategies to enhance biofuel yields are discussed. The review also addresses large-scale algal biomass cultivation for biofuel production, assesses its commercial viability, examines challenges faced by the biofuel industry, and outlines prospects for biofuel production using highly potent algal strains. By overcoming and addressing these challenges, algal biofuels have the potential to become a cornerstone of sustainable energy solutions.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"521-534"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of a salt-tolerant Coelastrum sp. and exploration of its potential for biodiesel production. 分离和鉴定一种耐盐鹅掌霉菌并探索其生产生物柴油的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-09-24 DOI: 10.1080/10826068.2024.2405941

Jing Xu, Han Wang, Jixin Liu, Jingping Ge, Yimeng Lin, Wenxiang Ping

引用次数: 0

Convenient production of a novel recombinant antibody against periodontitis biomarker S100A8. 便捷地制备针对牙周炎生物标志物 S100A8 的新型重组抗体。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-11-20 DOI: 10.1080/10826068.2024.2430615

Hui-Seon Yun, Eun-Jung Kim, Byung-Gee Kim, Hee-Jin Jeong

引用次数: 0

Enhanced production of yellow fever virus through tailored culture media optimization. 通过量身定制的培养基优化提高黄热病病毒的产量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-26 DOI: 10.1080/10826068.2024.2366990

Hareesh Reddy Narreddy, Ratna Prakash Kondapalli, T C Venkateswarulu

{"title":"Enhanced production of yellow fever virus through tailored culture media optimization.","authors":"Hareesh Reddy Narreddy, Ratna Prakash Kondapalli, T C Venkateswarulu","doi":"10.1080/10826068.2024.2366990","DOIUrl":"10.1080/10826068.2024.2366990","url":null,"abstract":"In the present study, an initial screening was conducted using 12 types of cell culture media, and four media with the best performance were selected for further study. The optimization of four media blend for YFV production was evaluated using an Augmented simplex centroid mixture design. Among all the different models that were investigated, the quadratic model was found to be the most appropriate model for exploring mixture design. It was found that M10 exhibited the greatest impact on YFV production, followed by M9, M4, and M1. The utilization of M1 and M4 media individually yielded higher compared to their blends with other media. The YFV titers were reduced when M1 media was combined with other media. The utilization of M9 and M10 media in combination resulted a higher viral yield compared to their respective concentrations. The optimal ratio for achieving a higher titer of YFV from primary CEFs was found to be approximately 38:62, with M9 and M10 being the most favorable media blend. The use of a media mixture led to a significant increase of virus titer up to 2.6 × 108 PFU/ml or 2 log titer yield, which is equivalent to 1.92 × 105 doses, without any changes to growth conditions or other process factors. This study concluded that the utilization of a mixture design could be efficiently employed to choose the optimal combination of media blends for enhanced viral production from cell culture.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"75-80"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141451260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulase production by Aspergillus fumigatus A4112 and the potential use of the enzyme in cooperation with surfactant to enhance floating oil recovery and methane production from palm oil mill effluent. 烟曲霉 A4112 产生的纤维素酶以及该酶与表面活性剂合作用于提高棕榈油厂污水的浮油回收率和甲烷产量的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-22 DOI: 10.1080/10826068.2024.2368627

Wiyada Khangkhachit, Wasana Suyotha, Sompong O-Thong, Poonsuk Prasertsan

{"title":"Cellulase production by Aspergillus fumigatus A4112 and the potential use of the enzyme in cooperation with surfactant to enhance floating oil recovery and methane production from palm oil mill effluent.","authors":"Wiyada Khangkhachit, Wasana Suyotha, Sompong O-Thong, Poonsuk Prasertsan","doi":"10.1080/10826068.2024.2368627","DOIUrl":"10.1080/10826068.2024.2368627","url":null,"abstract":"This research performed cellulase production by Aspergillus fumigatus A4112 and evaluated its potential use in palm oil mill effluent (POME) hydrolysis to recover oil simultaneously with the generation of fermentable sugar useful for biofuel production under non-sterilized conditions. Empty fruit bunch (EFB) without pretreatment was used as carbon source. The combination of nitrogen sources facilitated CMCase production. The maximum activity (3.27 U/mL) was obtained by 1.0 g/L peptone and 1.5 g/L (NH4)2SO4 and 20 g/L EFB at 40 °C for 7 days. High level of FPase activity (39.51 U/mL) was also obtained. Interestingly, the enzyme retained its cellulase activities more than 60% at ambient temperature over 15 days. In enzymatic hydrolysis, Triton X-100 was an effective surfactant to increase total oil recovery in the floating form. High yield of reducing sugar (50.13 g/L) and 21% (v/v) of floating oil was recoverable at 65 °C for 48 h. Methane content of the raw POME increased from 41.49 to 64.94% by using de-oiled POME hydrolysate which was higher than using the POME hydrolysate (59.82%). The results demonstrate the feasibility of the constructed process for oil recovery coupled with a subsequent step for methane yield enhancement in biogas production process that benefits the palm oil industry.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"100-111"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0