Preparative Biochemistry & Biotechnology最新文献_第10页

Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate. 以麦麸为低成本底物，通过固态发酵法利用真菌 Pleurotus djamor 生产凝乳蛋白酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-02 DOI: 10.1080/10826068.2024.2399040

Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira

{"title":"Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate.","authors":"Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira","doi":"10.1080/10826068.2024.2399040","DOIUrl":"https://doi.org/10.1080/10826068.2024.2399040","url":null,"abstract":"Proteases are enzymes that hydrolyze peptide bonds present in proteins and peptides. They are widely used for various industrial applications, such as in the detergent, food, and dairy industries. Cheese is one of the most important products of the dairy industry, and the coagulation stage is crucial during the cheese-making process. Enzymatic coagulation is the most common technique utilized for this purpose. Microbial enzymes are frequently used for coagulation due to their advantages in terms of availability, sustainability, quality control, product variety, and compliance with dietary and cultural/religious requirements. In the present study, we identified and subsequently characterized milk coagulant activity from the fungus Pleurotus djamor PLO13, obtained during a solid-state fermentation process, using the agro-industrial residue, wheat bran, as the fermentation medium. Maximum enzyme production and caseinolytic activity occurred 120 h after cultivation. When the enzyme activity against various protease-specific synthetic substrates and inhibitors was analyzed, the enzyme was found to be a serine protease, similar to elastase 2. This elastase-2-like serine protease was able to coagulate pasteurized whole and reconstituted skim milk highly efficiently in the presence and absence of calcium, even at room temperature. The coagulation process was influenced by factors such as temperature, time, and calcium concentration. We demonstrate here, for the first time, an elastase-2-like enzyme in a microorganism and its potential application in the food industry for cheese production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-7"},"PeriodicalIF":2.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of monoterpenes using Streptomyces strains from the rhizosphere of Inga edulis Martius from in an Amazonian urban forest fragment. 利用来自亚马逊城市森林片区 Inga edulis Martius 根圈的链霉菌株对单萜烯进行生物转化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-12 DOI: 10.1080/10826068.2024.2315476

Elison de Souza Sevalho, Rafael de Souza Rodrigues, Antonia Queiroz Lima de Souza, Afonso Duarte Leão de Souza

{"title":"Biotransformation of monoterpenes using Streptomyces strains from the rhizosphere of Inga edulis Martius from in an Amazonian urban forest fragment.","authors":"Elison de Souza Sevalho, Rafael de Souza Rodrigues, Antonia Queiroz Lima de Souza, Afonso Duarte Leão de Souza","doi":"10.1080/10826068.2024.2315476","DOIUrl":"10.1080/10826068.2024.2315476","url":null,"abstract":"To investigate the biocatalytic potential of Amazonian actinomycetes for monoterpenes biotransformation. To carry out the present study, eleven actinomycetes of the genus Streptomyces isolated from inga-cipó (Inga edulis Mart.) rhizospheres were tested for their ability to bioconvert the substrates R-(+)-limonene, S-(-)-limonene, 1S-(-)-α-pinene, and (-)-β-pinene as sole carbon and energy source. According to gas chromatography-mass spectrometry analysis, three strains, LabMicra B270, LaBMicrA B310, and LaBMicrA B314, were able to biotransform 1S-(-)-α-pinene after 96 h of growth. However, Streptomyces LaBMicrA B270 was the most promising since it converted after only 72 h all the 1S-(-)-α-pinene mainly into cis-verbenol (74.9±1.24%) and verbenone (18.2±1.20%), compounds that have important biological activities and great industrial interest as additives in foods and cosmetics. These findings can stimulate the development of natural aromas using naturally abundant monoterpenes, ratify the potential of microorganisms from almost unexplored niches such as the Amazonian rhizosphere, and reinforce the importance of preserving those niches.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1051-1057"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of various light spectra on amino acids and pigment production of Arthrospira platensis using flat-plate photobioreactor. 利用平板式光生物反应器研究不同光谱对板浆节肢动物氨基酸和色素产量的影响

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2021-07-21 DOI: 10.1080/10826068.2021.1941102

Hanieh Tayebati, Farshid Pajoum Shariati, Neda Soltani, Hessam Sepasi Tehrani

{"title":"Effect of various light spectra on amino acids and pigment production of Arthrospira platensis using flat-plate photobioreactor.","authors":"Hanieh Tayebati, Farshid Pajoum Shariati, Neda Soltani, Hessam Sepasi Tehrani","doi":"10.1080/10826068.2021.1941102","DOIUrl":"10.1080/10826068.2021.1941102","url":null,"abstract":"Today, the use of nutrients derived from natural bioactive compounds application in the food, pharmaceutical, and cosmetic industries is on the increase. This paper aimed to evaluate the amino acids profile (essential and non-essential) and pigments composition (chlorophyll a, carotenoids, and phycocyanin) of Arthrospira platensis (a blue-green microalga) cultivation in a flat-plate photobioreactor under various types of light-emitting diodes (red: 620-680 nm, white: 380-780 nm, yellow: 570-600nm, blue: 445-480 nm). The maximum biomass concentration (604.96 mg L-1) occurred when the red LED was applied for cultivation, and the minimum biomass concentration (279.39 mg L-1) was obtained under blue LED. The sequence of pigments and amino acids concentrations (mg L-1culture volume) was approximately in accordance with the biomass productivity. It means the red light produces the maximum concentration of pigments (chlorophyll a: 5.42, carotenoids: 2.92, phycocyanin: 67.54 mg L-1) and amino acids (essential amino acids: 110.47, nonessential amino acids: 179.10 mg L-1). Nevertheless, when these values were measured in mg per g of dry weight, the utmost contents were observed in microalgal products cultivated under blue LED. These consequences are due to the highest cell productivity and the most extended length of cells that occurred under red and blue LEDs, respectively.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1028-1039"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39210075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-12 DOI: 10.1080/10826068.2024.2325245

引用次数: 0

Development of a real-time PCR protocol for the detection of chicken DNA in meat products. 开发用于检测肉制品中鸡肉 DNA 的实时 PCR 程序。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-12 DOI: 10.1080/10826068.2024.2317289

Gulyaim Abitayeva, Arman Abeev

{"title":"Development of a real-time PCR protocol for the detection of chicken DNA in meat products.","authors":"Gulyaim Abitayeva, Arman Abeev","doi":"10.1080/10826068.2024.2317289","DOIUrl":"10.1080/10826068.2024.2317289","url":null,"abstract":"Food falsification is a pressing issue in today's food industry, with fraudulent substitution of costly ingredients with cheaper alternatives occurring globally. Consequently, developing straightforward and efficient diagnostic systems to detect such fraud is a top priority in scientific research. The aim of the work was to develop a test system and protocol for polymerase chain reaction (PCR) to detect in food products of animal origin the substitution of expensive meat raw materials for by-products of poultry processing. For this, real-time polymerase chain reaction (RT-PCR) was used, which allows determining the qualitative and quantitative substitution in raw and technologically prepared products. Other methods for detecting falsification - enzyme immunoassay (ELISA/ELISA) or express methods in the form of a lateral flow immunoassay are less informative. The extraction of nucleic acids for real-time polymerase chain reaction depends on the source matrix, with higher concentrations obtained from germ cells and parenchymal organs. Extraction from muscle and plant tissues is more challenging, but thorough grinding of these samples improves nucleic acid concentration by 1.5 times using DNA extraction kits. The selection of primers and fluorescent probes through GenBank and PCR Primer Design/DNASTAR software enables efficient amplification and identification of target chicken DNA fragments in various matrices.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1068-1078"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production. 含有木糖异构酶和木糖转运体的工程酿酒酵母能改善木糖和葡萄糖的共同发酵以生产乙醇。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-02-13 DOI: 10.1080/10826068.2024.2315479

Mengtian Huang, Xinxin Cui, Peining Zhang, Zhuocheng Jin, Huanan Li, Jiashu Liu, Zhengbing Jiang

{"title":"Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production.","authors":"Mengtian Huang, Xinxin Cui, Peining Zhang, Zhuocheng Jin, Huanan Li, Jiashu Liu, Zhengbing Jiang","doi":"10.1080/10826068.2024.2315479","DOIUrl":"10.1080/10826068.2024.2315479","url":null,"abstract":"Saccharomyces cerevisiae cannot assimilate xylose, second to glucose derived from lignocellulosic biomass. Here, the engineered S. cerevisiae strains INVSc-XI and INVSc-XI/XT were constructed using xylA and Xltr1p to co-utilize xylose and glucose, achieving economic viability and sustainable production of fuels. The xylose utilization rate of INVSc-XI/XT was 2.3-fold higher than that of INVSc-XI, indicating that overexpressing Xltr1p could further enhance xylose utilization. In mixed sugar media, a small amount of glucose enhanced the consumption of xylose by INVSc-XI/XT. Transcriptome analysis showed that glucose increased the upregulation of acetate of coenzyme A synthetase (ACS), alcohol dehydrogenase (ADH), and transketolase (TKL) gene expression in INVSc-XI/XT, further promoting xylose utilization and ethanol yield. The highest ethanol titer of 2.91 g/L with a yield of 0.29 g/g at 96 h by INVSc-XI/XT was 56.9% and 63.0% of the theoretical ethanol yield from glucose and xylose, respectively. These results showed overexpression of xylA and Xltr1p is a promising strategy for improving xylose and glucose conversion to ethanol. Although the ability of strain INVSc-XI/XT to produce ethanol was not very satisfactory, glucose was discovered to influence xylose utilization in strain INVSc-XI/XT. Altering the glucose concentration is a promising strategy to improve the xylose and glucose co-utilization.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1058-1067"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system. 在大肠杆菌表达系统中生产来自莫隆尼鼠白血病病毒的逆转录酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-02-27 DOI: 10.1080/10826068.2024.2317311

Yudhi Nugraha, Fina Amreta Laksmi, Isa Nuryana, Helbert, Firyal Nida Khasna

{"title":"Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system.","authors":"Yudhi Nugraha, Fina Amreta Laksmi, Isa Nuryana, Helbert, Firyal Nida Khasna","doi":"10.1080/10826068.2024.2317311","DOIUrl":"10.1080/10826068.2024.2317311","url":null,"abstract":"Reverse transcriptase (RT) is one of the most important enzymes used in molecular biology applications, enabling the conversion of RNA into complementary DNA (cDNA) that is used in reverse transcription-polymerase chain reaction (RT-PCR). The high demand of RT enzymes in biotechnological applications making the production optimization of RT is crucial for meeting the growing demand in industrial settings. Conventionally, the expression of recombinant RT is T7-induced promoter using IPTG in Escherichia coli expression systems, which is not cost-efficient. Here, we successfully made an alternative procedure for RT expression from Moloney murine leukemia virus (M-MLV) using autoinduction method in chemically defined medium. The optimization of carbon source composition (glucose, lactose, and glycerol) was analyzed using Response Surface Methodology (RSM). M-MLV RT was purified for further investigation on its activity. A total of 32.8 mg/L purified M-MLV RT was successfully obtained when glucose, glycerol, and lactose were present at concentration of 0.06%, 0.9%, and 0.5% respectively, making a 3.9-fold improvement in protein yield. In addition, the protein was produced in its active form by displaying 7462.50 U/mg of specific activity. This study provides the first step of small-scale procedures of M-MLV RT production that make it a cost-effective and industrially applicable strategy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1079-1087"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitinase induction in Trichoderma harzianum: a solid-state fermentation approach using shrimp waste and wheat bran/commercial chitin for chitooligosaccharides synthesis. 诱导哈茨毛霉中的几丁质酶：利用虾废料和麦麸/商用几丁质合成壳寡糖的固态发酵方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-02-12 DOI: 10.1080/10826068.2024.2313631

Cynthia Lizbeth López-García, Guadalupe Guerra-Sánchez, Fortunata Santoyo-Tepole, Dario Rafael Olicón-Hernández

{"title":"Chitinase induction in Trichoderma harzianum: a solid-state fermentation approach using shrimp waste and wheat bran/commercial chitin for chitooligosaccharides synthesis.","authors":"Cynthia Lizbeth López-García, Guadalupe Guerra-Sánchez, Fortunata Santoyo-Tepole, Dario Rafael Olicón-Hernández","doi":"10.1080/10826068.2024.2313631","DOIUrl":"10.1080/10826068.2024.2313631","url":null,"abstract":"This study innovatively employed solid-state fermentation (SSF) to evaluate chitinase induction in Trichoderma harzianum. Solid-state fermentation minimizes water usage, a crucial global resource, and was applied using shrimp waste chitin and a mixture of commercial chitin with wheat bran as substrates. Shrimp waste and wheat bran were pretreated and characterized for SSF, and the fungus's utilization of the substrates was assessed using spectrophotometric and microscopic methods. The resulting enzymes' ability to produce chitooligosaccharides (COS) mixtures was studied. Wheat bran/commercial chitin demonstrated superior performance, with a 1.8-fold increase in chitinase activity (76.3 U/mg protein) compared to shrimp waste chitin (41.8 U/mg protein). Additionally, the COS mixture obtained from wheat bran/commercial chitin showed a higher concentration of reducing sugars, reaching 87.85 mM, compared to shrimp waste chitin (14.87 mM). The COS profile from wheat bran/commercial chitin included monomers to heptamers, while the profile from shrimp waste chitin was predominantly composed of monomers. These results highlight the advantages of SSF for chitinase induction and COS production in T. harzianum, offering potential applications as dietary fiber, antioxidants, and antimicrobial agents. The findings contribute to by-product valorization, waste reduction, and the sustainable generation of valuable products through SSF-based enzyme production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1040-1050"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tyrosinase from the pulps of local cultivars of Musa spp: Purification, characterization, immobilization, and application in the batch production of l-3,4-dihydroxyphenylalanine. 从当地种植的麝香树果肉中提取的酪氨酸酶纯化、表征、固定化以及在批量生产 l-3,4-二羟基苯丙氨酸中的应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-06 DOI: 10.1080/10826068.2024.2324084

Muinat Moronke Adeyanju, Adedeji Nelson Ademakinwa

{"title":"Tyrosinase from the pulps of local cultivars of Musa spp: Purification, characterization, immobilization, and application in the batch production of l-3,4-dihydroxyphenylalanine.","authors":"Muinat Moronke Adeyanju, Adedeji Nelson Ademakinwa","doi":"10.1080/10826068.2024.2324084","DOIUrl":"10.1080/10826068.2024.2324084","url":null,"abstract":"Tyrosinase, an enzyme involved in browning reactions in plants/crops exposed to mechanical injury, was isolated from the pulp of some different locally available bananas (M. cavendish, M. acuminata, and M. paradisiaca). Tyrosinase from the pulps was extracted, purified, immobilized, and characterized. Thereafter, the potentials of the immobilized tyrosinase in the possible production of l-3,4-dihydroxyphenylalanine (L-DOPA) in an improvised batch reactor was exploited using tyrosine and ascorbate as the substrates. L-DOPA production was monitored via thin-layer chromatography and spectrophotometry (Arnow's method). L-DOPA is a drug that is used in the treatment of Parkinson's disease. Hence, this study exploited a non-chemical route for its synthesis using the tyrosinase obtained from the banana pulps. The purified tyrosinase had an optimum pH and temperature of 6.5 and 7.0, respectively. The molecular weight of the purified tyrosinase was 45 kDa. Quercetin and resorcinol both competitively inhibited the purified tyrosinase from the three cultivars. Immobilized M. cavendish tyrosinase produced the highest concentration (0.60 mM) of L-DOPA after 8 h in an improvised batch reactor. The tyrosinase in the banana pulps serves as a cheap and readily available green route for the possible production of L-DOPA.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1098-1105"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140040158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of media using food-grade components for the fermentation of Bifidobacterium and Lactobacillus strains in large-scale bioreactors. 使用食品级成分对培养基进行改良，以便在大型生物反应器中发酵双歧杆菌和乳酸杆菌菌株。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-01 Epub Date: 2021-01-06 DOI: 10.1080/10826068.2020.1861009

Chayanee Boontun, Savitri Vatanyoopaisarn, Sungwarn Hankla, Eisuke Kuraya, Yasutomo Tamaki

{"title":"Modification of media using food-grade components for the fermentation of Bifidobacterium and Lactobacillus strains in large-scale bioreactors.","authors":"Chayanee Boontun, Savitri Vatanyoopaisarn, Sungwarn Hankla, Eisuke Kuraya, Yasutomo Tamaki","doi":"10.1080/10826068.2020.1861009","DOIUrl":"10.1080/10826068.2020.1861009","url":null,"abstract":"Probiotic bacteria continue to receive increasing attention in the food and feed industries. However, the production of Bifidobacterium and Lactobacillus at an industrial scale is challenging because of specific nutrient requirements and conditions, which are complicated and costly. We developed low-cost culture media by modifying the carbon and nitrogen sources for Bifidobacterium animalis subsp. lactis KMP-H9-01 and Lactobacillus reuteri KMP-P4-S03 from available food grade components. Sucrose (15 g/l) was selected as a suitable carbon source for both strains because it was the most economic and facilitated bacterial growth that was equal to that of glucose. The Bifidobacterium strain required beef extract as a nitrogen source to multiply. The fermentation of both strains using the modified media formula in 5-L and 50-L bioreactors showed that the highest cell counts of L. reuteri and B. animalis subsp. lactis were 9 and 9.8 log CFU/ml after 12-15 h, respectively. The concentration (g/l) ratio between lactate and acetate obtained from B. animalis subsp. lactis was 7:7.4 at 12 h and 11.4:10.6 at 40 h; the ratio was similar at both time points (6.9: 1.1) for L. reuteri. Thus, this economically modified food-grade medium for the large-scale fermentation of two probiotic bacteria was efficient.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1017-1027"},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38785445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0