Preparative Biochemistry & Biotechnology最新文献_第10页

A state-of-the-art in exploring microalgal vitamins: Advancements in production, extraction, market potential, applications, and perspectives for a sustainable future. 探索微藻维生素的最新进展：生产、提取、市场潜力、应用和可持续未来的前景。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2026-02-07 DOI: 10.1080/10826068.2026.2625200

Sourav Maity, Alok Kumar Yadav

{"title":"A state-of-the-art in exploring microalgal vitamins: Advancements in production, extraction, market potential, applications, and perspectives for a sustainable future.","authors":"Sourav Maity, Alok Kumar Yadav","doi":"10.1080/10826068.2026.2625200","DOIUrl":"10.1080/10826068.2026.2625200","url":null,"abstract":"Microalgae offer a sustainable and adaptable source of essential vitamins, making them a promising alternative to traditional synthetic and plant-based sources. As natural and bioavailable nutrients become increasingly important, microalgae such as Chlorella, Spirulina, Dunaliella, and Haematococcus, known for their ability to synthesize a wide range of vitamins, including A, B, C, D, E, and K, are gaining more attention. The biosynthesis pathways of these vitamins are explored, with a focus on stress-induced techniques and the impact of genetic engineering on production yields. Recent innovations in photobioreactors and hybrid open-closed systems, as well as advanced extraction methods, are explored for their potential to enhance efficiency and sustainability in vitamin recovery. Their antioxidant, anti-inflammatory, and immune-boosting properties make microalgal vitamins valuable tools for addressing global nutritional deficiencies and improving overall health. Despite significant progress, challenges persist, including high production costs, scalability issues, and regulatory concerns. The review emphasizes the importance of developing cost-effective farming systems, advanced genetic engineering, and modern extraction technologies, and suggests directions for future research. Vitamins from microalgae hold great potential for transforming the global nutraceutical and pharmaceutical industries, fostering innovation, creating revenue opportunities, and promoting a more sustainable and eco-friendly future.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"331-358"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and characterization of three alginate lyases from Vibrio sp. E: targeting kelp hydrolysis and biofilm disruption. 以海带水解和生物膜破坏为目标的三种海藻酸解酶的表达和特性研究。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-07-16 DOI: 10.1080/10826068.2025.2525209

Yu Wang, Xilei Xu, Wenqian Wu, Juan Gao, Yumei Li

引用次数: 0

Sequential extraction, structural characterization, and biological activities of polysaccharides from olive (Olea europaea L.) pomace. 橄榄果渣多糖的序列提取、结构表征及生物活性研究。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-06-04 DOI: 10.1080/10826068.2025.2512946

Xiao-Liang Zhao, Kai-Li Qi, Hui Han, Hai-Dong Wen, Yan-Mei Liu, Qing-Song Zhou, Yuan-Yuan Ma, Cun-Jin Wang, Jing Zhang, Ji Zhang, Wei-Jie Zhang

{"title":"Sequential extraction, structural characterization, and biological activities of polysaccharides from olive (Olea europaea L.) pomace.","authors":"Xiao-Liang Zhao, Kai-Li Qi, Hui Han, Hai-Dong Wen, Yan-Mei Liu, Qing-Song Zhou, Yuan-Yuan Ma, Cun-Jin Wang, Jing Zhang, Ji Zhang, Wei-Jie Zhang","doi":"10.1080/10826068.2025.2512946","DOIUrl":"10.1080/10826068.2025.2512946","url":null,"abstract":"Olive pomace, a by-product of olive oil production, remains underexplored despite its potential environmental and economic benefits. This study sequentially extracted three polysaccharides (OERC, OERH, and OERA) from olive pomace using water at room temperature, hot water (80 °C), and 2% Na2CO3 solution (60 °C). Characterization through UV-Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, high-performance liquid chromatography, and Congo red experiment revealed that these polysaccharides are heteropolysaccharides primarily composed of glucose, mannose, rhamnose, and galactose, each with distinct molar ratios. The polysaccharides exhibited significant antioxidant activity by scavenging hydroxyl, DPPH, and superoxide radicals, with OERC showing the highest potency. They also repaired H2O2-induced oxidative damage in HepG2 cells, with OERC at 400 μg/mL and OERH/OERA at 200 μg/mL displaying optimal efficacy. Additionally, these polysaccharides effectively inhibited α-glucosidase activity, enhanced glucose consumption, and increased glycogen content in insulin-resistant models, thereby exerting hypoglycemic effects. Furthermore, they promoted proliferation, phagocytosis, and nitric oxide release in RAW264.7 macrophages, demonstrating immunomodulatory effects. These results indicate that sequential extraction under varying conditions is an effective method for preparing polysaccharides. The three polysaccharides isolated in this study show great potential for development as functional products with antioxidant, hypoglycemic, and immunomodulatory applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"45-61"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response surface methodology optimization extraction of polysaccharide from Lilium brownii F.E. Brown var. viridulum Baker/Longya Baihe and its biological activities. 响应面法优化龙牙百合多糖提取工艺及其生物活性。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-09-11 DOI: 10.1080/10826068.2025.2556871

Menglu Li, Ruotong He, Ceyao Yang, Hao Chen, Chaoling Wu, Xiaomiao Zhang, Xin Fu, Chengbo Lu, Liu Yang, Shi Liu, Yi Zhang

{"title":"Response surface methodology optimization extraction of polysaccharide from Lilium brownii F.E. Brown var. viridulum Baker/Longya Baihe and its biological activities.","authors":"Menglu Li, Ruotong He, Ceyao Yang, Hao Chen, Chaoling Wu, Xiaomiao Zhang, Xin Fu, Chengbo Lu, Liu Yang, Shi Liu, Yi Zhang","doi":"10.1080/10826068.2025.2556871","DOIUrl":"10.1080/10826068.2025.2556871","url":null,"abstract":"Lilium brownii F.E.Brown var. viridulum Baker (Longya Baihe), a traditional medicinal and edible plant. In order to study the activity of its polysaccharide extracts, a three-factor three-level Box-Behnken response surface design was employed to optimize the extraction process. The optimal extraction conditions include: liquid-solid ratio of 32:1 mL/g, extraction temperature of 70 °C, and extraction time of 3.5 h. The purified Longya Baihe polysaccharide (LP), obtained via DEAE-52 cellulose chromatography, was structurally characterized by molecular weight distribution, monosaccharide composition and infrared spectrum. The results show that LP was a macromolecular polysaccharide composed of glucose and mannose (molar ratio of 1:1.56), with an average molecular mass (Mn) of 24.006 kDa. In vitro assays demonstrated its excellent antioxidant and anti-inflammatory activities. LP significantly inhibited lipopolysaccharide (LPS)-induced activation of the nuclear factor kappa-B (NF-κB) pathway in RAW264.7 cells, downregulated the overexpression of nitric oxide (NO), interleukin (IL)-6, inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α, and enhancing the levels of the anti-inflammatory factor interleukin (IL)-10. This study suggests LP holds promise as a natural antioxidant and anti-inflammatory agent for potential development and application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"593-607"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and characterization of terpolymer P(3HB-co-3HV-co-4HB) produced by Cupriavidus malaysiensis USMAA1020 using glycerol pitch. 以甘油酯为原料合成马来铜枝USMAA1020共聚物P（3HB-co-3HV-co-4HB）的生物合成及表征

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-07-05 DOI: 10.1080/10826068.2025.2522473

Musa Ibn Abbas, Nur Hanani Arif Shah, Amirul Al Ashraf Abdullah, Siti Nor Syairah Anis

{"title":"Biosynthesis and characterization of terpolymer P(3HB-co-3HV-co-4HB) produced by Cupriavidus malaysiensis USMAA1020 using glycerol pitch.","authors":"Musa Ibn Abbas, Nur Hanani Arif Shah, Amirul Al Ashraf Abdullah, Siti Nor Syairah Anis","doi":"10.1080/10826068.2025.2522473","DOIUrl":"10.1080/10826068.2025.2522473","url":null,"abstract":"This study demonstrates a circular economy approach by utilizing glycerol pitch, biodiesel co-product as a carbon source to produce biodegradable terpolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) [P(3HB-co-3HV-co-4HB)] using Cupriavidus malaysiensis USMAA1020. Various carbon sources, including oleic acid, 1,4-butanediol, and 1-pentanol, were combined with glycerol pitch as main substrates. The experiments were conducted in a mineral salt medium (MSM) with a high concentration of carbon source and limited nitrogen, which promotes both bacterial growth and PHA accumulation. Preliminary studies indicated, the composition of the polymer was triggered by varying the concentration of 1,4-butanediol, oleic acid, and glycerol pitch while keeping the amount of 1-pentanol constant. After optimization, the PHA content in dry biomass increased from 60 ± 12 to 88 ± 5 wt%, while the residual dry cell weight (RDCW) slightly decreased from 1.77 ± 0.53 to 1.11 ± 0.41 g/L. The combination of glycerol pitch in the biosynthesis of the terpolymer proved effective for the cost-efficient and sustainable production of P(3HB-co-3HV-co-4HB). This process could drive innovation while reducing waste, by harnessing glycerol pitch's properties, sustainable co-product of biodiesel production into a valuable biodegradable polymer. The characterization of the terpolymer revealed a diverse range of properties, making it suitable for various applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"111-123"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microencapsulation of astaxanthin-rich lipid extract from L. vannamei cephalothorax in calcium alginate matrix. 藻酸钙基质中富虾青素脂质提取物的微胶囊化研究。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-08-14 DOI: 10.1080/10826068.2025.2512942

Alynne Rodriges Silva, Juliana Rabelo Sousa, Men de Sá Moreira Souza Filho, Adriano Lincoln Albuquerque Mattos, André Luís Coelho Silva, Fabrízia da Silva Quaresma, Bartolomeu Warlene Silva Souza

{"title":"Microencapsulation of astaxanthin-rich lipid extract from L. vannamei cephalothorax in calcium alginate matrix.","authors":"Alynne Rodriges Silva, Juliana Rabelo Sousa, Men de Sá Moreira Souza Filho, Adriano Lincoln Albuquerque Mattos, André Luís Coelho Silva, Fabrízia da Silva Quaresma, Bartolomeu Warlene Silva Souza","doi":"10.1080/10826068.2025.2512942","DOIUrl":"10.1080/10826068.2025.2512942","url":null,"abstract":"Crustacean residues are potential sources of carotenoids, bioactive molecules with antioxidant and anti-inflammatory activities. The lipid extract from shrimp consists mainly of astaxanthin and unsaturated fatty acids with high nutraceutical potential. However, the chemical instability of astaxanthin represents a drawback that needs to be overcome to enable the application in food products. Therefore, this work aimed to obtain microcapsules from the lipid extract of L. vannamei cephalothorax in a calcium alginate matrix. The extraction method showed relevant technical and economic viability, according to the results of yield and astaxanthin content. The lipid extract showed excellent antioxidant potential, given the predominant concentration of astaxanthin and oleic acid in the chemical constitution of the extract. The microencapsulation method used resulted in microcapsules with morphology and size suitable for use in food matrices.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"24-33"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144856162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review of herb microgreens as emerging functional foods: insights into their nutritional potential and health benefits. 草药微蔬菜作为新兴功能食品的综合综述：对其营养潜力和健康益处的见解。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-05-29 DOI: 10.1080/10826068.2025.2511840

P K Rasmi, Chandrakant Genu Dalbhagat, Arun Prasath Venugopal, Sabyasachi Mishra, Nanje Gowda N A, Vivek Kambhampati

{"title":"A comprehensive review of herb microgreens as emerging functional foods: insights into their nutritional potential and health benefits.","authors":"P K Rasmi, Chandrakant Genu Dalbhagat, Arun Prasath Venugopal, Sabyasachi Mishra, Nanje Gowda N A, Vivek Kambhampati","doi":"10.1080/10826068.2025.2511840","DOIUrl":"10.1080/10826068.2025.2511840","url":null,"abstract":"Microgreens are young, immature plants widely used in salads and dishes for their nutritional value, color, texture, and flavor. Their compatibility with indoor and small-scale cultivation makes them a unique crop of modern controlled environment agriculture, addressing the growing demand of urban populations. Beyond their culinary appeal, microgreens are gaining recognition as nutrient-dense functional foods due to their high concentrations of vitamins, minerals, antioxidants, and bioactive compounds, including flavonoids, carotenoids, and phenolics, compared to mature greens and sprouts. Unlike sprouts, which are harvested after 2-7 days, microgreens are harvested between 7 and 21 days after the development of young true leaves, offering enhanced microbial safety. Among them, herb microgreens such as fenugreek, basil, mustard, fennel, coriander, and moringa stand out for their rich bioactive composition and potential therapeutic benefits. Their health-promoting properties include anti-hyperglycemic, anti-carcinogenic, and anti-inflammatory effects, and roles in immune modulation, cardiovascular health, and managing age-related diseases. As a result, their demand is increasing in both the food and pharmaceutical industries. This review explores the nutritional profile, therapeutic significance, human health perspectives, and culinary applications of various herb microgreens. Additionally, this review highlights the key research gaps and future directions to optimize their functional food potential for human health.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling molecular mechanisms of enhanced pectinase production in Paecilomyces sp. via ARTP mutagenesis and transcriptome analysis. 通过ARTP诱变和转录组分析揭示拟青霉增强果胶酶生产的分子机制。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-12-29 DOI: 10.1080/10826068.2025.2568071

Hongxi Chen, Xianzhen Li

{"title":"Unraveling molecular mechanisms of enhanced pectinase production in Paecilomyces sp. via ARTP mutagenesis and transcriptome analysis.","authors":"Hongxi Chen, Xianzhen Li","doi":"10.1080/10826068.2025.2568071","DOIUrl":"10.1080/10826068.2025.2568071","url":null,"abstract":"Microbial pectinases are extensively utilized in food processing, textile bio-scouring, and agricultural waste valorization. In this study, a wild-type Paecilomyces sp. C11 strain with pectinase activity (110 U/mL) was isolated from Changbai Mountain forest soil. Using atmospheric and room-temperature plasma (ARTP) mutagenesis, we generated a high-yielding mutant (C21) exhibiting a 129.1% increase in pectinase yield (242 U/mL). Comparative transcriptomics of C11 and C21 identified 1982 differentially expressed genes (DEGs), with 541 upregulated and 1441 downregulated. Key DEGs linked to pectinase biosynthesis and secretion-including VPS29 (vacuolar sorting), GH28 (polygalacturonase), Sec7 (vesicle trafficking), GCS1 (transcriptional regulation), D-xylulokinase, and alcohol dehydrogenase-were validated via RT-qPCR. Functional enrichment confirmed ribosome biogenesis, carbohydrate metabolism, and transmembrane transport as critical pathways facilitating hyperproduction. These findings provide actionable targets for rational engineering of Paecilomyces strains and establish ARTP as a robust tool for industrial enzyme optimization.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"615-625"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of biodegradable polyhydroxybutyrate PHB plastic through the combination of eco-friendly materials by Bacillus cereus B52. 蜡样芽孢杆菌B52与环保材料的结合，提高了可生物降解聚羟基丁酸酯PHB塑料的产量。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-07-21 DOI: 10.1080/10826068.2025.2533443

Alfa Zulkarnain, Mulyorini Rahayuningsih, Ni Putu Ratna Ayu Krishanti, Fateha Fateha, Rossy Choreun Nissa, Kharis Yohan Abidin, Yeyen Nurhamiyah

{"title":"Enhanced production of biodegradable polyhydroxybutyrate PHB plastic through the combination of eco-friendly materials by Bacillus cereus B52.","authors":"Alfa Zulkarnain, Mulyorini Rahayuningsih, Ni Putu Ratna Ayu Krishanti, Fateha Fateha, Rossy Choreun Nissa, Kharis Yohan Abidin, Yeyen Nurhamiyah","doi":"10.1080/10826068.2025.2533443","DOIUrl":"10.1080/10826068.2025.2533443","url":null,"abstract":"Polyhydroxybutyrate (PHB) is an environmentally friendly polymer that is synthesized by bacteria and stored as a carbon source. One of the main problems in PHB production is the price of materials for production. This study aims to optimize environmentally friendly and affordable materials such as molasses, tofu wastewater, and coconut water to increase PHB yield. Statistical optimization with central composite design (CCD) was used to optimize materials in Bacillus cereus B52 (B. cereus B52) for PHB production. The concentration of molasses, tofu wastewater and coconut water were used as optimization parameters to increase the PHB yield. The optimization results resulted in the production of PHB yield of 1.45 ± 0.14 g/L. Chemical analysis was used to determine the biopolymer validation of PHB. In conclusion, production optimization using a combination of materials from molasses, tofu wastewater, and coconut water can increase PHB production in B. cereus B52.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"359-369"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and extraction kinetic modeling of antioxidants, polyphenols, and flavonoids from Garcinia cambogia and Myristica fragrans rind. 藤黄果和肉豆蔻果皮中抗氧化剂、多酚和黄酮类化合物的提取动力学模型及优化。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2026-01-01 Epub Date: 2025-08-20 DOI: 10.1080/10826068.2025.2547192

Drisya Raj M P, Sukumar M

{"title":"Optimization and extraction kinetic modeling of antioxidants, polyphenols, and flavonoids from Garcinia cambogia and Myristica fragrans rind.","authors":"Drisya Raj M P, Sukumar M","doi":"10.1080/10826068.2025.2547192","DOIUrl":"10.1080/10826068.2025.2547192","url":null,"abstract":"The optimization for the extraction of bioactive compounds from Garcinia cambogia (GC) and Myristica fragrans (nutmeg rind (NM)) using Peleg's kinetic model with Response Surface Methodology (RSM) was observed. The extraction efficiency was assessed through Total Phenolic Content (TPC), Total Flavonoids (TF), and Antioxidant activity. The effect of particle size, temperature, and solvent concentration on extraction performance was facilitated by RSM. The kinetics of both initial rapid solute release and eventual equilibrium phase were carried out using Peleg's model. The model demonstrated high predictive accuracy of TPC values with R2 = 0.985 and Root Mean Square Error (RSME) = 0.90 μg Gallic Acid Equivalence/mL (μg GAE/mL) and TF values with R2 = 0.907 and RSME = 0.752 μg Quercetin Equivalence/mL (μg QE/mL) and R2 = 0.961 and RSME = 0.316% for antioxidant activity for G. cambogia. Similarly, a satisfactory fit was observed for M. fragrans (R2 = 0.971; RMSE = 11.19 μg GAE/mL), (R2 = 0.0.571 and RSME = 0.266 μg QE/mL), and (R2 = 0.923 and RSME = 0.511%), respectively. The close agreement between observed and predicted values supports the robustness of Peleg's model and RSM in optimizing extraction parameters.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"467-476"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0