Preparative Biochemistry & Biotechnology最新文献_第10页

Soluble and insoluble expression of recombinant human interleukin-2 protein using pET expression vector in Escherichia coli. 使用 pET 表达载体在大肠杆菌中可溶和不可溶地表达重组人白细胞介素-2 蛋白。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-02 DOI: 10.1080/10826068.2024.2361146

Atif Ahmed, Nao Akusa Fujimura, Saad Tahir, Muhammad Akram, Zaheer Abbas, Maira Riaz, Ali Raza, Rabia Abbas, Nadeem Ahmed

{"title":"Soluble and insoluble expression of recombinant human interleukin-2 protein using pET expression vector in Escherichia coli.","authors":"Atif Ahmed, Nao Akusa Fujimura, Saad Tahir, Muhammad Akram, Zaheer Abbas, Maira Riaz, Ali Raza, Rabia Abbas, Nadeem Ahmed","doi":"10.1080/10826068.2024.2361146","DOIUrl":"https://doi.org/10.1080/10826068.2024.2361146","url":null,"abstract":"Interleukin-2 has emerged as a potent protein-based drug to treat various cancers, AIDS, and autoimmune diseases. Despite its immense requirement, the production procedures are inefficient to meet the demand. Therefore, efficient production procedures must be adopted to improve protein yield and decrease procedural loss. This study analyzed cytoplasmic and periplasmic IL-2 expression for increased protein yield and significant biological activity. The study is focused on cloning IL-2 into a pET-SUMO and pET-28a vector that expresses IL-2 in soluble form and inclusion bodies, respectively. Both constructs were expressed into different E. coli expression strains, but the periplasmic and cytoplasmic expression of IL-2 was highest in overnight culture in Rosetta 2 (DE3). Therefore, E. coli Rosetta 2 (DE3) was selected for large-scale production and purification. Purified IL-2 was characterized by SDS-PAGE and western blotting, while its biological activity was determined using MTT bioassay. The results depict that the periplasmic and cytoplasmic IL-2 achieved adequate purification, yielding 0.86 and 0.51 mg/mL, respectively, with significant cytotoxic activity of periplasmic and cytoplasmic IL-2. Periplasmic IL-2 has shown better yield and significant biological activity in vitro which describes its attainment of native protein structure and function.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":2.9,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring traditional and modern approaches for extracting bioactive compounds from Ferulago trachycarpa. 探索从阿魏（Ferulago trachycarpa）中提取生物活性化合物的传统和现代方法。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-17 DOI: 10.1080/10826068.2024.2349937

Shakeel Ahmed, Nilofar, Aleksandra Cvetanović Kljakić, Alena Stupar, Biljana Lončar, Jelena Božunović, Uroš Gašić, Evren Yıldıztugay, Claudio Ferrante, Gokhan Zengin

{"title":"Exploring traditional and modern approaches for extracting bioactive compounds from Ferulago trachycarpa.","authors":"Shakeel Ahmed, Nilofar, Aleksandra Cvetanović Kljakić, Alena Stupar, Biljana Lončar, Jelena Božunović, Uroš Gašić, Evren Yıldıztugay, Claudio Ferrante, Gokhan Zengin","doi":"10.1080/10826068.2024.2349937","DOIUrl":"https://doi.org/10.1080/10826068.2024.2349937","url":null,"abstract":"For more than two millennia, Ferulago species have been revered as therapeutic herbs, maintaining their significance in present-day folk medicine practices. Therefore, the present study was conducted to investigate the phytochemical composition, inhibitory effects on metabolic enzymes, and possible therapeutic applications of F. trachycarpa, specifically focusing on its efficacy in diabetes management, anticholinergic effects, and antioxidant capabilities. The current investigation comprised an evaluation of a range of extracts acquired via conventional and modern methodologies, such as soxhlet (SOX), maceration (MAC) accelerated solvent extraction (ASE), homogenizer-assisted extraction (HAE), supercritical fluid extraction (SFE), microwave-assisted extraction (MW), and ultrasound-assisted extraction (UAE). Various techniques were employed to assess their antioxidant capacity and enzyme inhibition. Furthermore, the research utilized ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) to ascertain the principal phenolic compounds that are responsible for the antioxidant capacity observed in the various F. trachycarpa extracts. Among these, extracts from HAE, ASE, and MW revealed the most promise across all methodologies tested for their antioxidant potential. Furthermore, SFE and MAC extracts inhibited the most enzymes, including cholinesterases, tyrosinase, α -amylase, and α -glycosidase, indicating their potential as efficient natural treatments for several health-related issues.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.9,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production, characterization, and bio-ethanologenic potential of a novel tripartite raw starch-digesting amylase from Priestia flexa UCCM 00132. 一种新的三元原料淀粉消化淀粉酶的生产、表征和生物产乙醇潜力。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-10-03 DOI: 10.1080/10826068.2023.2259452

David Sam Ubi, Maurice George Ekpenyong, Eloghosa Joyce Ikharia, Ernest Ablewho Akwagiobe, Atim David Asitok, Sylvester Peter Antai

{"title":"Production, characterization, and bio-ethanologenic potential of a novel tripartite raw starch-digesting amylase from Priestia flexa UCCM 00132.","authors":"David Sam Ubi, Maurice George Ekpenyong, Eloghosa Joyce Ikharia, Ernest Ablewho Akwagiobe, Atim David Asitok, Sylvester Peter Antai","doi":"10.1080/10826068.2023.2259452","DOIUrl":"10.1080/10826068.2023.2259452","url":null,"abstract":"The biological conversion of agro-waste biomass into value-added metabolites is one of the trendy biotechnological research areas in recent times. One of the major drawbacks of the bioprocess is the saccharification potential of the amylolytic enzyme that releases reducing sugar from complex biomass to serve as substrate for fermentation. The present study reports the production of a novel tripartite raw starch-digesting amylase (RSDA) by an indigenous Priestia flexa strain with α-, β-, and gluco-amylolytic activities and its potential for bioethanol production. Response surface statistics was employed to develop a suitable medium for improved production of the tripartite enzyme by submerged fermentation. The bioprocess selected raw starch (4.36%) Ca2+(2.71 g/L) and Zn2+ (0.0177 g/L) as significant variables which demonstrated a total RSDA activity of 7208.23 U/mL in a 5-L batch bioreactor. SDS/Native-PAGE determined the molecular weights of the 27-fold purified product as 25.2 kDa, 57.3 kDa, and 90.1 kDa for α-, β-, and gluco-amylases, respectively. Optimum temperature and pH for enzyme activity were respectively broad at 30-70 °C and 4-11. The enzyme mixture demonstrated digestibility above 90% against a variety of raw starches and simultaneous fermentation of digestate with Saccharomyces cerevisiae generated 71.69 g/L of bioethanol within 24 h suggesting great potential for bioethanologenesis.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"597-611"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41140550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of collagenolytic proteases from Aspergillus heteromorphus URM 0269 in an aqueous two-phase system for application in collagen hydrolysis. 在双水相系统中从异形曲霉URM 0269中提取胶原分解蛋白酶用于胶原水解。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-10-10 DOI: 10.1080/10826068.2023.2263870

Beatriz de Aquino Marques da Costa, Ana Cláudia Vaz de Araújo, Lígia Maria Gonçalves Fernandes, Ana Lúcia Figueiredo Porto, Vagne de Melo Oliveira, Tatiana Souza Porto

{"title":"Extraction of collagenolytic proteases from Aspergillus heteromorphus URM 0269 in an aqueous two-phase system for application in collagen hydrolysis.","authors":"Beatriz de Aquino Marques da Costa, Ana Cláudia Vaz de Araújo, Lígia Maria Gonçalves Fernandes, Ana Lúcia Figueiredo Porto, Vagne de Melo Oliveira, Tatiana Souza Porto","doi":"10.1080/10826068.2023.2263870","DOIUrl":"10.1080/10826068.2023.2263870","url":null,"abstract":"Collagenolytic proteases produced by Aspergillus heteromorphus URM0269 were extracted using a PEG/sulfate aqueous two-phase system (ATPS). A 23 factorial design was performed to analyze the independent variables: PEG molar mass (MPEG), PEG concentration (CPEG), and sulfate concentration (Csulf). The extracted proteases were also evaluated for their optimum pH and stability at different pH levels (4.0 - 11.0) after 20 h of incubation. Collagen was extracted from mutton snapper (Lutjanus analis) skin using acetic acid (0.5 mol L-1). The enzyme was preferentially partitioned to the PEG-rich phase (K > 1), whose highest purification factor and recovery (PF = 6.256 and Y = 404.432%) were obtained under specific conditions: MPEG 8000 g.mol-1, CPEG 30%, Csulf 10%. The ATPS extraction provided an enzymatic activity range of pH 7.0 - 11.0, exhibiting greater stability compared to the crude extract. Approximately 80% of protease activity was maintained after 20 hours of incubation at all analyzed pH levels, except pH 11.0. Collagen extraction from L. analis skin yielded 8.056%, and both crude extract samples and ATPS-derived samples successfully hydrolyzed the extracted collagen, reaching peak hydrolysis after 36 hours of treatment. These findings demonstrate the feasibility of extracting highly purified and active proteases capable of hydrolyzing L. analis collagen.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"647-659"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-level production of poly-γ-glutamic acid by a newly isolated Bacillus sp. YJY-8 and potential use in increasing the production of tomato. 新分离的芽孢杆菌YJY-8高效生产聚γ-谷氨酸及其在番茄增产中的潜在应用。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-09-28 DOI: 10.1080/10826068.2023.2261058

Fuming He, Baojun Gao, Xin Cheng, Jiao Zhai, Xinqing Zhang, Chuanlun Yang, Tian Jiewei

{"title":"High-level production of poly-γ-glutamic acid by a newly isolated Bacillus sp. YJY-8 and potential use in increasing the production of tomato.","authors":"Fuming He, Baojun Gao, Xin Cheng, Jiao Zhai, Xinqing Zhang, Chuanlun Yang, Tian Jiewei","doi":"10.1080/10826068.2023.2261058","DOIUrl":"10.1080/10826068.2023.2261058","url":null,"abstract":"Strain YJY-8, a new γ-polyglutamic acid producer, was separated from fermented soybean paste samples. The strain was identified as a genus of Bacillus by morphological and 16S rDNA sequence analysis and was named Bacillus sp. YJY-8. The optimal medium composition and cultural conditions were studied using a single-factor experiment and a response surface experiment. The optimized medium consisted of monosodium glutamate 70 g/L, glucose 54.3 g/L, glycerol 31.8 g/L, ammonium sulfate 11.1 g/L, yeast extract 3.2 g/L, tryptone 1.5 g/L, L-glutamic acid 6.8 g/L, MgSO4 7H2O 0.5 g/L, FeCl3 6H2O 0.02 g/L, KH2PO4 0.9 g/L, CaCl2 0.03 g/L, MnSO4 H2O 0.3 g/L, ammonium molybdate 0.02 g/L, pH 7.0. The optimal cultivation conditions were 35 °C and pH 7.0. Under the optimized conditions, after 48 hr of cultivation, the highest shaking flask fermentation level of γ-PGA reached 65.2 ± 0.36 g/L. In addition, through fed-batch fermentation in 30 L fermenters, the fermentation level of γ-PGA reached its highest level at 88.42 g/L and productivity was 1.23 g/(L hr) after 72 hr. Then, the effect of γ-PGA on tomato yield was investigated. At the seedling stage, the plant height and stem diameter of γ-PGA treated plants increased by 5.69 and 15.735% after spraying γ-PGA for 19 days. During the flowering and fruiting period, the stem diameter of the γ-PGA treatment group increased by 6.74%, with a maximum increase of 11.65%. The number of fruit branches increased by 0.56-16.29% and the number of fruit sets increased by 1.01-28.47%. At the fruit maturation stage, the yield of tomatoes increased by 10.51, 14.27, and 5.83%.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"637-646"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41102160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of phenolic compounds from walnut green husk (Juglans regia L.) by Salting-Out extraction method. 用盐析法提取核桃青皮中的酚类化合物。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-11-10 DOI: 10.1080/10826068.2023.2273481

Sorour Barekat, Ali Nasirpour, Javad Keramat, Mohammad Dinari, Ali Sedaghat Doost, Paul Van der Meeren

{"title":"Extraction of phenolic compounds from walnut green husk (Juglans regia L.) by Salting-Out extraction method.","authors":"Sorour Barekat, Ali Nasirpour, Javad Keramat, Mohammad Dinari, Ali Sedaghat Doost, Paul Van der Meeren","doi":"10.1080/10826068.2023.2273481","DOIUrl":"10.1080/10826068.2023.2273481","url":null,"abstract":"Some factors in the salting-out extraction (SOE) method play a major role. The aim of this study was to investigate the interaction effects of the phase forming components and consequently select the best conditions to achieve a highly efficient recovery of phenolic compounds from walnut green husks (Juglans regia L.) using mixtures of ethanol and aqueous ammonium sulfate solutions. According to the results that were analyzed by response surface methodology, the optimal extraction conditions were obtained at ethanol: salt: water ratio of 34.8: 15.1: 54.4 (w/w) at a pH of 6-6.5 and 25 °C. At the optimal conditions, the overall phenolic and flavonoid content, and antioxidant activity were significantly higher than obtained by the conventional method. In addition, at a higher scale (i.e., 5 kg), similar results were obtained. Thus, it can be concluded that SOE has the potential to be scaled up for the simultaneous separation and purification of compounds from plant biomass. This paper is addressing extraction techniques, measurement, and characterization of new natural phenolic compounds from an agricultural by-product and valorization of waste.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"680-690"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72210511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and characterization of extracellular tannase from Aspergillus fumigatus MA using Syzigium cumini leaves under solid state fermentation. 利用孜然Syzigium叶在固态发酵条件下纯化烟曲霉细胞外单宁酶并对其进行鉴定。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-11-10 DOI: 10.1080/10826068.2023.2279106

Krishan Kumar Selwal, Manjit K Selwal

{"title":"Purification and characterization of extracellular tannase from Aspergillus fumigatus MA using Syzigium cumini leaves under solid state fermentation.","authors":"Krishan Kumar Selwal, Manjit K Selwal","doi":"10.1080/10826068.2023.2279106","DOIUrl":"10.1080/10826068.2023.2279106","url":null,"abstract":"This study reports the tannase purification produced by a tannery effluent-originated fungal isolate i.e., Aspergillus fumigatus MA under solid state fermentation (SSF) condition. Purification of tannase from culture filtrate was attained using ammonium sulfate precipitation with subsequent diethylaminoethyl (DEAE)-cellulose mediated ion exchange chromatographic technique. Fractional precipitation of the culture filtrate with 60-80% ammonium sulfate yielded 80.9% recovery of tannase with 6.16-fold purification. The enzyme fractions were collected and eluted as a single peak using 0.5 M NaCl-gradient concentration. DEAE-cellulose column chromatography results in overall 23-fold purification with 27.6% recovery of the enzyme. SDS-PAGE analysis of purified tannase confirmed the presence of a single band of protein with a molecular mass equivalent to 66.2 kDa. The highest activity of tannase was observed at optimum pH ranged between 5.0-6.0 whereas, the tannase stability (>80%) was observed at 4.0 to 7.0 pH ranges. The purified tannase activity was found to be optimally active at 30 °C whereas stability (>90%) was accomplished between 30-50 °C temperature. The Km and Vmax were found to be 1.61 × 10-3 M and 1.04 mM respectively. These properties suggest the potential of the enzyme to be utilized in various food, feed, and pharmaceutical sectors.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"720-727"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of the sugar fraction from Arabica coffee pulp as a carbon source for bacteria producing cellulose and cytotoxicity with human keratinocyte. 利用阿拉比卡咖啡浆中的糖部分作为细菌产生纤维素的碳源，并对人类角质形成细胞产生细胞毒性。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-09-25 DOI: 10.1080/10826068.2023.2258195

Jiraporn Sangta, Warintorn Ruksiriwanich, Chuda Chittasupho, Korawan Sringarm, Pornchai Rachtanapun, Cassie Bakshani, William Willats, Sarana Sommano

{"title":"Utilization of the sugar fraction from Arabica coffee pulp as a carbon source for bacteria producing cellulose and cytotoxicity with human keratinocyte.","authors":"Jiraporn Sangta, Warintorn Ruksiriwanich, Chuda Chittasupho, Korawan Sringarm, Pornchai Rachtanapun, Cassie Bakshani, William Willats, Sarana Sommano","doi":"10.1080/10826068.2023.2258195","DOIUrl":"10.1080/10826068.2023.2258195","url":null,"abstract":"Coffee pulp (CP), a by-product of coffee production, is an underutilized resource with significant potential value. CP contains monosaccharides that can serve as an ideal carbon source for bacterial cultivation, enabling the production of value-added components such as medical-grade cellulose. Herein, we extracted the sugar fraction from Arabica CP and used it as a supplement in a growing media of a bacteria cellulose (BC), Komagataeibacter nataicola. The BC was then characterized and tested for cytotoxicity. The CP sugar fraction yielded approximately 7% (w/w) and contained glucose at 4.52 mg/g extract and fructose at 7.34 mg/g extract. Supplementing the sugar fraction at different concentrations (0.1, 0.3, 0.5, 0.7, and 1 g/10 mL) in sterilized glucose yeast extract broth, the highest yield of cellulose (0.0020 g) occurred at 0.3 g/10 mL. It possessed similar physicochemical attributes to the BC using glucose, with some notable improvements in fine structure and arrangement of the functional groups. In cytotoxicity assessments on HaCaT keratinocyte cells, bacterial cellulose concentrations of 2-1000 µg/mL exhibited viability of ≥ 80%. However, higher concentrations were toxic. This research innovatively uses coffee pulp for bacterial cellulose, aligning with the principles of a bio-circular economy that focuses on sustainable biomass utilization.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"587-596"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41138848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of bacterial cellulose production by ethanol and lactic acid by using Gluconacetobacter kombuchae. 利用康普茶葡糖酸杆菌提高乙醇和乳酸对细菌纤维素的生产。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-11-08 DOI: 10.1080/10826068.2023.2276188

Poonam Sharma, Ritu Sharma, Simran Ahuja, Anita Yadav, Sanjiv Arora, Neeraj K Aggarwal

{"title":"Enhancement of bacterial cellulose production by ethanol and lactic acid by using Gluconacetobacter kombuchae.","authors":"Poonam Sharma, Ritu Sharma, Simran Ahuja, Anita Yadav, Sanjiv Arora, Neeraj K Aggarwal","doi":"10.1080/10826068.2023.2276188","DOIUrl":"10.1080/10826068.2023.2276188","url":null,"abstract":"The current study intended to analyze the impact of ethanol and lactic acid on the bacterial cellulose yield as well as physicochemical and mechanical properties, by using Gluconacetobacter kombuchae. The optimization of ethanol and lactic acid concentration has been done by using one-way ANOVA. Both the supplements significantly enhance the yield of bacterial cellulose (BC) as compared to the standard Hestrin-Schramm medium (control). Optimization leads to significant increase in BC yield as compared to the control, i.e., the addition, of optimized concentration of lactic acid (0.6%) increases the yield from (0.78 ± 0.026) g to (4.89 ± 0.020) g dry weight, and optimized concentration of ethanol (1%) increases the yield from (0.73 ± 0.057) g to (3.7 ± 0.01) g dry weight. Various physicochemical and mechanical properties of BC films produced in different media (i.e., HS, HS + Ethanol, and HS + Lactic acid), such as the crystallinity, structure, tensile strength, strain at break, Young's modulus, and water holding capacity, were also examined, by employing various techniques such as SEM, FTIR, XRD, etc. BC produced in medium supplemented with the optimum concentration of both the additives were found to possesses higher porosity. Though, slight decline in crystallinity was observed. But the tensile strength and strain at break, were upgraded 1.5-2.5 times, 2-2.5 times, respectively. This article attempted to present a method for enhancing BC yields and characteristics that may lead to more widespread and cost-effective use of this biopolymer.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"700-708"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71485205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous fermentation using high cell density cell recycle system for L-lactic acid production. 利用高细胞密度细胞循环系统进行连续发酵，生产 L-乳酸。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-05-01 Epub Date: 2024-01-08 DOI: 10.1080/10826068.2023.2268207

Vaishali Gupta, Annamma A Odaneth, Arvind M Lali

引用次数: 0