Preparative Biochemistry & Biotechnology最新文献_第10页

Isolation, purification, and physio-chemical characterization of melanin pigment from nigerseed hulls (Guizotia abyssinica). 从黑木耳（Guizotia abysinica）中分离、提纯和鉴定黑色素。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-07-12 DOI: 10.1080/10826068.2024.2376579

Sunil Kumar B T, Umesh H Hebbar, Sridevi Annapurna Singh

{"title":"Isolation, purification, and physio-chemical characterization of melanin pigment from nigerseed hulls (Guizotia abyssinica).","authors":"Sunil Kumar B T, Umesh H Hebbar, Sridevi Annapurna Singh","doi":"10.1080/10826068.2024.2376579","DOIUrl":"10.1080/10826068.2024.2376579","url":null,"abstract":"Plant melanin, a natural pigment, has gained significant attention recently due to its potential therapeutic and industrial applications. In this study, melanin pigments were extracted from Nigerseed hulls (NH) via alkali and acid extraction methods, followed by acid hydrolysis, organic solvent treatment, and repeated precipitation. The solubility of NH melanin was assessed, revealing solubility in alkali and dimethyl sulfoxide (DMSO) but insolubility in other common organic solvents. High-performance liquid chromatography (HPLC) was employed to measure the purity of NH melanin in comparison to standard melanin, while elemental analysis indicated a similarity between melanin extracted from nigerseed hulls and the standard counterpart. LC-MS data revealed a molecular weight of NH melanin. Furthermore, the stability of melanin was evaluated under varying conditions including temperature, oxidants, reducing agents, light exposure, and metal ion presence. Results demonstrated significant effects of Mg2+, Cu2+, and Fe2+ metal ions on melanin stability, with a minor effect observed for Ca2+, while sodium hyposulfite was found to destabilize the pigments. Our findings suggest that nigerseed hulls hold promise as a novel source for efficient melanin production, with potential applications in the food sector, food packaging, and biomedical fields.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"141-149"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced temperature control in ethanol fermentation using a PSO-PID controller with split-range control strategy. 采用分程控制策略的 PSO-PID 控制器在乙醇发酵过程中实现先进的温度控制。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-08-03 DOI: 10.1080/10826068.2024.2381761

Raju Yerolla, Suhailam P, Chandra Shekar Besta

{"title":"Advanced temperature control in ethanol fermentation using a PSO-PID controller with split-range control strategy.","authors":"Raju Yerolla, Suhailam P, Chandra Shekar Besta","doi":"10.1080/10826068.2024.2381761","DOIUrl":"10.1080/10826068.2024.2381761","url":null,"abstract":"Global energy demand is experiencing a notable surge due to growing energy security. Renewable energy sources, like ethanol, are becoming more viable. In the present study, the application of a PSO-PID (Particle Swarm Optimization - Proportional Integral Derivative) controller with a split-range control strategy was suggested for the regulation of temperature within the fermentation system. To optimize performance, a POS-PID controller with a split-range arrangement utilizing two control valves for hot and cold utilities was constructed. The study began by examining the open-loop dynamic response of the system to inlet temperature and concentration disturbances during ethanol production fermentation. Subsequently, a transfer function model was developed through linearization at the steady-state operating point. The split-range controller structure, implemented by optimizing the PSO-PID controller parameters using PSO, effectively demonstrated temperature control in simulations of a nonlinear model. In this investigation, the ethanol fermentation system was modeled as a CSTR using a modified Monod equation for microbial growth kinetics. Various dynamic behavioral disturbances were explored and verified in the model with plant data in this study. The simulation model results were validated through plant data. The proposed method showed superior closed-loop performance with respect to errors, with the actuators proving to be effective than other reported methods for temperature control.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"196-209"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the impact of sodium acetate on lipid and carotenoid production in Rhodotorula mucilaginosa. 探讨醋酸钠对粘红酵母脂质和类胡萝卜素产生的影响。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-01-06 DOI: 10.1080/10826068.2024.2441918

Gedela Ravi, Veeranki Venkata Dasu, Kannan Pakshirajan

{"title":"Exploring the impact of sodium acetate on lipid and carotenoid production in Rhodotorula mucilaginosa.","authors":"Gedela Ravi, Veeranki Venkata Dasu, Kannan Pakshirajan","doi":"10.1080/10826068.2024.2441918","DOIUrl":"10.1080/10826068.2024.2441918","url":null,"abstract":"The study employed batch shake flasks to evaluate the impact of various nitrogen sources, phosphate levels, and sodium acetate (Na-acetate) on the Rhodotorula mucilaginosa growth and metabolite production. Adding Na-acetate to the medium resulted in significant improvements in critical metabolites. In shake flask experiments, this led to a cell dry weight (CDW) of 1.65 ± 0.94 g L-1, with lipids comprising 66.53% of the biomass. While β-carotene and carotenoid were 5.84 ± 0.05 and 37.66 ± 2.13 µg g-1, respectively. Subsequent experiments in a batch reactor with Na-acetate supplementation further improved these metrics. CDW increased to 5.02 ± 0.83 g L-1, and lipid content to 65.73 ± 0.81%. Carotenoid production rose to 40.33 ± 1.84 µg g-1, with β-carotene reaching 17.63 ± 0.32 µg g-1. The most promising results were obtained using a fed-batch bioreactor strategy with Na-acetate. R. mucilaginosa achieved the highest yields across all parameters: 48.36 ± 1.14 µg g-1 of total carotenoids, 21.38 ± 1.14 µg g-1 of β-carotene, and a lipid content of 68.58 ± 1.95%.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"590-605"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel. 最大限度地提高多酚产量：芒果皮的超声波辅助提取和抗菌潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-10-02 DOI: 10.1080/10826068.2024.2411518

Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri

{"title":"Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel.","authors":"Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri","doi":"10.1080/10826068.2024.2411518","DOIUrl":"10.1080/10826068.2024.2411518","url":null,"abstract":"This study investigated the ultrasound-assisted extraction (UAE) techniques used to enhance the polyphenols and antioxidants of mango peel extract (MPE). Additionally, it explored the bacteriostatic activity of MPE against various microorganisms. The UAE method was optimized using response surface methodology (RSM) at different times, temperatures, and ratios, with optimal conditions found to be 35 minutes, 45 °C, and a 1:35 ratio. The optimized yield results for total polyphenol content (TPC) were 17.33 ± 1.57 mg GAE/g, total flavonoid content (TFC) was 12.14 ± 0.29 mg QE/g, and radical scavenging activity (RSA) was 72.11 ± 2.19%. These response models were extremely significant with p-values less than 0.05. MPE showed selective effectiveness against Bacillus cereus, Geobacillus stearothermophilus, and Escherichia coli (E. coli). The results highlight the potential of mango peel as a sustainable source of bioactive compounds, contributing to waste reduction in the food industry and the development of natural antimicrobial agents. This study contributes to further research on the application of MPE in processed foods.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"349-358"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promising approaches in the extraction, characterization, and biotechnological applications of ursolic acid: a review. 熊果酸的提取、表征及生物技术应用研究进展

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-03-15 DOI: 10.1080/10826068.2025.2475094

Vijayalakshmi K, Sonali L, Kanimozhi N V, Pavithra R, Drisya Raj M P, Chinta Suneetha, R Palpandi Raja, Sukumar Muthusamy

{"title":"Promising approaches in the extraction, characterization, and biotechnological applications of ursolic acid: a review.","authors":"Vijayalakshmi K, Sonali L, Kanimozhi N V, Pavithra R, Drisya Raj M P, Chinta Suneetha, R Palpandi Raja, Sukumar Muthusamy","doi":"10.1080/10826068.2025.2475094","DOIUrl":"10.1080/10826068.2025.2475094","url":null,"abstract":"Ursolic acid (UA), a pentacyclic triterpenoid, has gained attention for its pharmacological properties and industrial uses. This study explores natural sources of UA, including Plumeria rubra, apple peels, sage, rosemary, and holy basil, while emphasizing sustainable extraction methods. Advanced techniques like Soxhlet extraction, solvent extraction, supercritical fluid extraction (SFE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE) are optimized to enhance yield and purity. Ethanol and methanol solvent extraction provide effective recovery, while SFE with supercritical CO2 increases selectivity and reduces solvent residue. EAE boosts efficiency by breaking down cell membranes, allowing sustained UA release. With antibacterial, anticancer, antidiabetic, and anti-inflammatory effects, UA holds promise in therapeutics and has applications in nutraceuticals, cosmetics, and food preservation. However, its low water solubility and bioavailability require innovative delivery methods like dendrimers and nanoparticles. This review merges traditional and modern approaches to UA extraction, bioavailability enhancement, and sustainable use, offering new perspectives on its potential in medicine, food technology, and cosmetics.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"973-984"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of zinc oxide nanoparticles using Tamarix chinensis extract: evaluation of antioxidant, antimicrobial, and anti-MCF-7 cancer activities. 柽柳提取物生物合成氧化锌纳米颗粒：抗氧化、抗菌和抗mcf -7抗癌活性的评价

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-12-31 DOI: 10.1080/10826068.2024.2446461

Diego Carlos Bouttier-Figueroa, Manuel Alfredo Loreto-Romero, Francisco Humberto González-Gutiérrez, Manuel Cortez-Valadez, Mario Flores-Acosta, Salvador Enrique Meneses-Sagrero, Ramón Enrique Robles-Zepeda

{"title":"Biosynthesis of zinc oxide nanoparticles using Tamarix chinensis extract: evaluation of antioxidant, antimicrobial, and anti-MCF-7 cancer activities.","authors":"Diego Carlos Bouttier-Figueroa, Manuel Alfredo Loreto-Romero, Francisco Humberto González-Gutiérrez, Manuel Cortez-Valadez, Mario Flores-Acosta, Salvador Enrique Meneses-Sagrero, Ramón Enrique Robles-Zepeda","doi":"10.1080/10826068.2024.2446461","DOIUrl":"https://doi.org/10.1080/10826068.2024.2446461","url":null,"abstract":"This study explores the green synthesis of zinc oxide nanoparticles (ZnONPs) using Tamarix chinensis extract, known for its hepatoprotective and anti-inflammatory phytochemicals. The ZnONPs were synthesized using ZnSO4 and NaOH, resulting in an average size of 125 nm, as confirmed by scanning electron microscopy (SEM). X-ray diffraction (XRD) analysis revealed a hexagonal crystalline structure. UV-Vis spectroscopy showed an absorption peak at 360 nm, corresponding to a bandgap of 3.37 eV, making these nanoparticles suitable for controlled drug delivery systems. The antioxidant activity of T. chinensis extract was evaluated using the DPPH assay, demonstrating an 80% inhibition of free radicals at 500 µg/mL, surpassing the antioxidant potential of the ZnONPs. The antimicrobial efficacy of the ZnONPs was evidenced by a minimum inhibitory concentration (MIC) of <50 µg/mL against Escherichia coli and Staphylococcus aureus. Additionally, the ZnONPs exhibited antiproliferative activity with an IC50 of 20.80 µg/mL in MCF-7 breast cancer cells, highlighting their potential for biomedical applications. These findings suggest the promising multifunctional role of biosynthesized ZnONPs in healthcare and technological innovations.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":"55 6","pages":"684-695"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous partitioning of multiple bioactive compounds from Garcinia indica rinds in a three-liquid-phase extraction systems. 在三液相萃取系统中同时分离黄藤皮中多种生物活性化合物。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-03-25 DOI: 10.1080/10826068.2025.2483240

Chaitra Chandrakant Shanbhag, Raini Mohd Hanzala Salimuddin, Regupathi Iyyaswami, Prasanna D Belur

{"title":"Simultaneous partitioning of multiple bioactive compounds from Garcinia indica rinds in a three-liquid-phase extraction systems.","authors":"Chaitra Chandrakant Shanbhag, Raini Mohd Hanzala Salimuddin, Regupathi Iyyaswami, Prasanna D Belur","doi":"10.1080/10826068.2025.2483240","DOIUrl":"10.1080/10826068.2025.2483240","url":null,"abstract":"Simultaneous extraction and purification of principal bioactive compounds, anthocyanins (ACNs), garcinol (GL), and hydroxycitric acid (HCA) from the rinds of Garcinia indica (Kokum) fruits in a single-step using Three Liquid Phase Systems (TLPS) were investigated. Among the various phase-forming components studied, TLPS formed by n-hexane-ethanol-(NH4)2SO4-water system was considered for partitioning GL into the n-hexane-rich top phase, ACNs into the ethanol-rich middle phase, and HCA into the aqueous salt-rich bottom phase. The present system was even able to separate carbohydrates into the bottom phase, which can be detrimental to the stability of ACNs. The effect of n-hexane, ethanol, and (NH4)2SO4 concentration on the partitioning behavior of biomolecules was analyzed. The TLPS composed of water-n-hexane-ethanol-(NH4)2SO4 could purify and extract 95.08% of ACNs, 95.33% of GL, and 67.98% of HCA in a single-step extraction process while the other extraction methods require multi-step extraction process to separate these three compounds. The effect of pH studies on the partitioning characteristics of biomolecules revealed that pH 4 is optimum and more efficient than the native pH of the system to achieve maximum yield of all the bioactive compounds.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1118-1134"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freeze-thaw assisted immobilization of Rhodococcus erythropolis MTCC 3951 in kappa carrageenan for efficient terephthalic acid biodegradation. 冻融辅助固定红球菌 MTCC 3951 在卡帕卡拉胶中高效降解对苯二甲酸。

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-04-07 DOI: 10.1080/10826068.2025.2487182

Ankita C Maurya, Amrik Bhattacharya, Sunil K Khare

{"title":"Freeze-thaw assisted immobilization of Rhodococcus erythropolis MTCC 3951 in kappa carrageenan for efficient terephthalic acid biodegradation.","authors":"Ankita C Maurya, Amrik Bhattacharya, Sunil K Khare","doi":"10.1080/10826068.2025.2487182","DOIUrl":"10.1080/10826068.2025.2487182","url":null,"abstract":"Terephthalic acid (TPA) is widely used in the production of polyethylene terephthalate (PET) plastics and is one of PET's degraded products. However, TPA poses environmental health risks due to its persistence and potential toxicity, and thus, it is referred to as one of the major environmental pollutants. The present study describes the application of immobilized Rhodococcus erythropolis MTCC 3951 cells for TPA biodegradation to develop a viable bioprocess. Kappa carrageenan (KC) matrix was used to immobilize the cells. The entrapment conditions were modified for maximum TPA degradation and minimum cell leakage. The entrapped cells were cryofreeze at -20 °C for 24 h, followed by thawing at 24 °C for 4 h. Cryofreezing was introduced to enhance the cell's stability and mechanical strength, required for effective reusability. The stability of the matrix was confirmed by FTIR, TGA, and FESEM. The cryofreeze entrapped cells completely degrade 5 mM TPA within 14 h of treatment. The immobilized cells could be reused for at least 10 repeated cycles without loss in degradation efficiency. The cryofreezing of immobilized cells in KC is a novel technique to enhance the system's stability. The developed bioprocess could be used efficiently to alleviate TPA pollution from the environment.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1155-1165"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, and application on inulin hydrolysis. 土曲霉URM4658菊粉酶在海藻酸钙微球中的固定化、生化特性和动力学/热力学参数的评价及其在菊粉水解中的应用

IF 1.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2025-04-21 DOI: 10.1080/10826068.2025.2486424

Wallace Ribeiro da Silva, Camila Fernanda de Aquino Luna, Joyce Gueiros Wanderley Siqueira, Jorge Vinícius Fernandes Lima Cavalcanti, Rodrigo Lira de Oliveira, Tatiana Souza Porto

{"title":"Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, and application on inulin hydrolysis.","authors":"Wallace Ribeiro da Silva, Camila Fernanda de Aquino Luna, Joyce Gueiros Wanderley Siqueira, Jorge Vinícius Fernandes Lima Cavalcanti, Rodrigo Lira de Oliveira, Tatiana Souza Porto","doi":"10.1080/10826068.2025.2486424","DOIUrl":"10.1080/10826068.2025.2486424","url":null,"abstract":"The present study aimed to immobilize an inulinase obtained from Aspergillus terreus URM4658 by entrapment in calcium alginate beads. The immobilization process yielded a satisfactory yield (92.72%) using 1.25% sodium alginate and 0.35 M CaCl2 with a curing time of 90 min. The immobilized enzyme exhibited optimum pH and temperature at 7.0 and 60 °C, respectively, showing an increased affinity for the substrate after the immobilization process, as evidenced by the decrease in Km compared to its free form. Moreover, the immobilized inulinase demonstrated good thermostability at 50 and 60 °C, as observed from the t1/2 (649.83-420.84 min) and D-values (2158.67-1398.00 min), respectively. The immobilized biocatalyst also exhibited good reusability, maintaining 92.73% of residual activity after 10 reaction cycles and no loss of activity after 30 days of storage. A continuous inulin hydrolysis operation in a packed bed reactor was performed using the immobilized inulinase, and a maximum release of total reducing sugars and nystose of 3.27 and 0.82 g L-1, respectively, was observed. The results indicate that an immobilized biocatalyst is a promising alternative for bioprocess involving inulin-rich feedstocks.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1145-1154"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate. 以麦麸为低成本底物，通过固态发酵法利用真菌 Pleurotus djamor 生产凝乳蛋白酶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-09-02 DOI: 10.1080/10826068.2024.2399040

Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira

{"title":"Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate.","authors":"Monizy da Costa Silva, Ricardo Bezerra Costa, Josiel Santos do Nascimento, Marta Maria Oliveira Dos Santos Gomes, Alexsandra Nascimento Ferreira, Luciano Aparecido Meireles Grillo, José Maria Rodrigues da Luz, Francis Soares Gomes, Hugo Juarez Vieira Pereira","doi":"10.1080/10826068.2024.2399040","DOIUrl":"10.1080/10826068.2024.2399040","url":null,"abstract":"Proteases are enzymes that hydrolyze peptide bonds present in proteins and peptides. They are widely used for various industrial applications, such as in the detergent, food, and dairy industries. Cheese is one of the most important products of the dairy industry, and the coagulation stage is crucial during the cheese-making process. Enzymatic coagulation is the most common technique utilized for this purpose. Microbial enzymes are frequently used for coagulation due to their advantages in terms of availability, sustainability, quality control, product variety, and compliance with dietary and cultural/religious requirements. In the present study, we identified and subsequently characterized milk coagulant activity from the fungus Pleurotus djamor PLO13, obtained during a solid-state fermentation process, using the agro-industrial residue, wheat bran, as the fermentation medium. Maximum enzyme production and caseinolytic activity occurred 120 h after cultivation. When the enzyme activity against various protease-specific synthetic substrates and inhibitors was analyzed, the enzyme was found to be a serine protease, similar to elastase 2. This elastase-2-like serine protease was able to coagulate pasteurized whole and reconstituted skim milk highly efficiently in the presence and absence of calcium, even at room temperature. The coagulation process was influenced by factors such as temperature, time, and calcium concentration. We demonstrate here, for the first time, an elastase-2-like enzyme in a microorganism and its potential application in the food industry for cheese production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"278-284"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0