Preparative Biochemistry & Biotechnology最新文献_第9页

Comparison of the efficiency of the Sec and Tat secretory pathways in the secretion of recombinant neurturin protein using de novo designed signal peptides. 利用全新设计的信号肽比较 Sec 和 Tat 分泌途径在重组新乌头素蛋白分泌中的效率。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-21 DOI: 10.1080/10826068.2024.2331203

Zahra Hajihassan, Mina Yazdi, Atiyeh Fadaie, Nooshin Akbarsemnani

{"title":"Comparison of the efficiency of the Sec and Tat secretory pathways in the secretion of recombinant neurturin protein using de novo designed signal peptides.","authors":"Zahra Hajihassan, Mina Yazdi, Atiyeh Fadaie, Nooshin Akbarsemnani","doi":"10.1080/10826068.2024.2331203","DOIUrl":"10.1080/10826068.2024.2331203","url":null,"abstract":"Since cytoplasmic expression of heterologous proteins with disulfide bonds leads to the formation of inclusion bodies in E. coli, periplasmic production is preferable. The N-terminal signal peptide attached to the secreted protein determines the type of secretory pathway through which the target protein is secreted; Sec, Tat, or SRP. The aim of this study was to design and compare two novel signal peptides for the secretion of recombinant neurturin (as a model) via the Sec and Tat pathways. For this purpose, we aligned the natural signal peptides from E. coli and Bacillus subtilis to identify the conserved amino acids and those with the highest repetition. The SignalP4.1 and TatP1.0 software were used to determine the secretion efficiency of the new signal peptides. The efficiency of new signal peptides was then evaluated and compared experimentally with two naturally used signal peptides. Quantitative analysis of Western blot bands showed that approximately 80% of the expressed neurturin was secreted into the periplasmic space by new signal peptides. Circular dichroism spectroscopy also confirmed the correct secondary structure of the secreted neurturin. In conclusion, these novel signal peptides can be used to secrete any other recombinant proteins to the periplasmic space of E. coli efficiently.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1157-1169"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140176123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of ultrasound to increase the catalytic activity of α-L-rhamnosidase. 利用超声波提高α-L-鼠李糖酶的催化活性。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-13 DOI: 10.1080/10826068.2024.2326877

Chen Qi, Le Li, Kunpeng Yu, Yanling Lin, Lijun Li

引用次数: 0

Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach. 利用土壤分离真菌生产生物活性酚类化合物：一种发酵方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-13 DOI: 10.1080/10826068.2024.2326882

Rathnaprabha Dharavath, Srividya A

{"title":"Employing soil isolated fungi for production of bioactive phenolic compounds: a fermentative approach.","authors":"Rathnaprabha Dharavath, Srividya A","doi":"10.1080/10826068.2024.2326882","DOIUrl":"10.1080/10826068.2024.2326882","url":null,"abstract":"An efficient method of solid-state fermentation (SSF) is reported for producing bioactive phenolic compounds using soil-isolated fungi. Antioxidant activity using a rapid DPPH (1,1-diphenyl-2-picryl hydrazyl), was employed to screen the 120 fungal isolates from soil. Aspergillus terreus 1, Aspergillus fumigatus, Aspergillus terreus 2, Penicillium citrinum, Aspergillus wentii1, Aspergillus wentii 2, Penicillium expansum and Penicillium granulatum were chosen, concerning their antioxidant activity and total phenolic content. These fungal strains were applied on agro residues viz. sugarcane bagasse, corn cob, rice straw, pea pod and wheat straw, to evaluate the release of phenolic compounds. The fermented extracts from various agro-residues showed good antioxidant activity against DPPH, ferric ion, and nitric oxide radicals. The highest antioxidant activity was observed in fermented extracts of sugarcane bagasse, followed by pea pod. Additionally, the total phenolic content in the fermented extracts positively correlated with antioxidant potential. This study highlights the significant potential of solid substrate fermentation using soil-isolated fungi and agro-residues to produce bioactive phenolic compounds with potent antioxidant properties. The utilization of SSF for the extraction of bioactive compounds from natural sources not only offers a clean and sustainable approach but also contributes to the valorization of agro-industrial residues.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1121-1131"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin-transferrin-selenium promote formation of tissue-engineered vascular grafts in early stage of culture. 胰岛素-转铁蛋白-硒可促进组织工程血管移植物早期培养的形成。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-28 DOI: 10.1080/10826068.2024.2333468

Xuheng Sun, Nannan Wang, Hongjing Jiang, Qing Liu, Cong Xiao, Jianyi Xu, Yindi Wu, Jingyi Mei, Shuting Wu, Zhanyi Lin

{"title":"Insulin-transferrin-selenium promote formation of tissue-engineered vascular grafts in early stage of culture.","authors":"Xuheng Sun, Nannan Wang, Hongjing Jiang, Qing Liu, Cong Xiao, Jianyi Xu, Yindi Wu, Jingyi Mei, Shuting Wu, Zhanyi Lin","doi":"10.1080/10826068.2024.2333468","DOIUrl":"10.1080/10826068.2024.2333468","url":null,"abstract":"To create tissue-engineered vascular grafts (TEVGs) in vitro, vascular smooth muscle cells (VSMCs) must function effectively and produce sufficient extracellular matrix (ECM) in a three-dimensional space. In this study, we investigated whether the addition of insulin-transferrin-selenium (ITS), a medium supplement, could enhance TEVG formation. PGA fabric was used as the scaffold, and 1% ITS was added to the medium. After two weeks, the tissues were examined using electron microscopy and staining. The ITS group exhibited a denser structure and increased collagen production. VSMCs were cultured in two dimensions with ITS and assessed for collagen production, cell growth, and glucose metabolism. The results showed that ITS supplementation increased collagen production, cell growth, glucose utilization, lactate production, and ATP levels. Furthermore, reducing the amount of fetal bovine serum (FBS) in the medium did not affect the TEVGs or VSMCs when ITS was present. In conclusion, ITS improves TEVG construction by promoting VSMCs growth and reducing the need for FBS.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1186-1195"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental validation off-line a nonlinear controller for removal of chromium using non-living cells of Yarrowia lipolytica. 利用脂肪溶解亚罗菌的非活细胞去除铬的离线非线性控制器的实验验证。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-27 DOI: 10.1080/10826068.2024.2329277

Vicente Peña Caballero, Pablo A López-Pérez, García Salas Oatna Georgina, Adan T Morales-Vargas

{"title":"Experimental validation off-line a nonlinear controller for removal of chromium using non-living cells of Yarrowia lipolytica.","authors":"Vicente Peña Caballero, Pablo A López-Pérez, García Salas Oatna Georgina, Adan T Morales-Vargas","doi":"10.1080/10826068.2024.2329277","DOIUrl":"10.1080/10826068.2024.2329277","url":null,"abstract":"The removal of hexavalent chromium [Cr (VI)] using non-living cells of Yarrowia lipolytica was investigated. Batch and continuous studies on removal of Cr (VI) achieved 97% and 99% removal from aqueous phase, respectively. The specific uptake values at pH of 2 in batch process were 40.73 ± 1.3 mg/g and 30.09 ± 0.23 mg/g on non-living cells, when 100 and 200 mg/L of metal Cr (VI) concentrations were used. In order to investigate the regulation of Cr (VI) under continuous operation based on reaction volume numerically a new class of feedback controller from structure polynomial was designed. The proposed methodology was used to an experimentally kinetic model for a removal Cr (VI) from Yarrowia lipolytica biomass was showed satisfactory closed-loop performance the proposed controller. Starting from an off-line optimization performed in simulation, we present the controller implementation, focussing on the methodology required to could be suitable for implementation in real time. In our experimental results, we highlight some discrepancies between simulation and reality despite these differences, the controller managed to perform convergence to removal Cr (VI). Finally, the results validated with off-line samples suggest that the proposed control could be suitable for in application in potential scenarios for wastewater treatment.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1147-1156"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of a salt-tolerant Coelastrum sp. and exploration of its potential for biodiesel production. 分离和鉴定一种耐盐鹅掌霉菌并探索其生产生物柴油的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-24 DOI: 10.1080/10826068.2024.2405941

Jing Xu, Han Wang, Jixin Liu, Jingping Ge, Yimeng Lin, Wenxiang Ping

引用次数: 0

Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment. 利用实验统计设计优化新型肠球菌的外多糖生产。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-20 DOI: 10.1080/10826068.2024.2402337

Shivani Singh Gaur, Uday S Annapure

{"title":"Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment.","authors":"Shivani Singh Gaur, Uday S Annapure","doi":"10.1080/10826068.2024.2402337","DOIUrl":"https://doi.org/10.1080/10826068.2024.2402337","url":null,"abstract":"The Exopolysaccharide (EPS) producing novel strains of Enterococcus previously isolated from the vaginal source of pregnant women were selected based on ropy structure formation. The two selected strains, E.villorum SB-2 and E.rivorum S22-3, were found to be producing 2.87 g/l and 3.14 g/l EPS, respectively, in the minimal media (M17 media) after 24-hour fermentation under anaerobic condition. Both the strains have probiotic properties and have the potential to be used for industrial applications. The production media and fermentation conditions were optimized to enhance the EPS production using the one-factor method, Placket-Burman factorial designing and Central composite design (CCD) of Response surface methodology (RSM). The most relevant factors affecting the EPS yield were sucrose, yeast extract and pH for E.villorum SB2 and sucrose, yeast extract and magnesium sulfate for the E.rivorum S22-3 as determined by Placket-Burman design, whose concentrations were further optimized using CCD. The optimized fermentation conditions gave the total EPS of 9.76 g/l (4 times the initial production) from E.villorum SB-2 and 7.74 g/l (2.5 times the initial production) from E.rivorum S22-3, respectively, after 36-hour incubation at 37 °C. These optimization studies might be helpful during scale-up process for the industrial scale production of these exopolysaccharide.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing methane yield and shifting microbial communities in anaerobic reactors treating lipid-rich dairy wastewater through exogenous lipase addition 通过添加外源脂肪酶提高处理富含脂质的乳制品废水的厌氧反应器中的甲烷产量并改变微生物群落

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10826068.2024.2399042

Marwa Abedalkarem, Omamah Dabbour, Sare Asli

引用次数: 0

Disposable glutamate biosensor based on platinum nanoparticles, carbon quantum dots and poly-L-aspartic acid. 基于铂纳米粒子、碳量子点和聚天冬氨酸的一次性谷氨酸生物传感器。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10826068.2024.2402340

Ceren Kaçar Selvi,Barış Şenceol,Pınar Esra Erden

{"title":"Disposable glutamate biosensor based on platinum nanoparticles, carbon quantum dots and poly-L-aspartic acid.","authors":"Ceren Kaçar Selvi,Barış Şenceol,Pınar Esra Erden","doi":"10.1080/10826068.2024.2402340","DOIUrl":"https://doi.org/10.1080/10826068.2024.2402340","url":null,"abstract":"This study reports the design and development of a disposable amperometric biosensor for the determination of L-glutamate. Glutamate oxidase (GlOx) was immobilized onto a screen-printed carbon electrode (SPE) modified with poly-L-Aspartic acid (PAsp), carbon quantum dots (CQD), and platinum nanoparticles (PtNP) for the construction of the biosensor. The surface composition of the modified SPE was optimized using the one variable at a time method. The morphological properties of the biosensor were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The electrochemical behavior of the modified electrodes was studied by cyclic voltammetry. Under the optimized experimental conditions the linear working range, detection limit and sensitivity of the GlOx/PtNP/CQD/PAsp/SPE were found to be 1.0 - 140 µM, 0.3 µM and 0.002 µA µM-1, respectively. The GlOx/PtNP/CQD/PAsp/SPE biosensor also exhibited good measurement repeatability. The as-developed biosensor was applied for the determination of L-glutamate in spiked serum samples and the average analytical recovery of added glutamate was 98.9 ± 3.9%.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":"6 1","pages":"1-9"},"PeriodicalIF":2.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142178366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2,3-Butanediol plus acetoin obtention by Enterobacter aerogenes ATCC 13048: inhibition by target products and cells reuse during fed-batch cultivation 产气肠杆菌 ATCC 13048 获取 2,3-丁二醇和乙炔酮：目标产品的抑制作用和喂料批次培养过程中细胞的再利用

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10826068.2024.2402341

Bruna Campos de Souza, Analia Borges Folle, Sabrina Carra, Eloane Malvessi

引用次数: 0