Preparative Biochemistry & Biotechnology最新文献_第9页

Soluble and insoluble expression of recombinant human interleukin-2 protein using pET expression vector in Escherichia coli. 使用 pET 表达载体在大肠杆菌中可溶和不可溶地表达重组人白细胞介素-2 蛋白。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2025-01-01 Epub Date: 2024-06-02 DOI: 10.1080/10826068.2024.2361146

Atif Ahmed, Nao Akusa Fujimura, Saad Tahir, Muhammad Akram, Zaheer Abbas, Maira Riaz, Ali Raza, Rabia Abbas, Nadeem Ahmed

{"title":"Soluble and insoluble expression of recombinant human interleukin-2 protein using pET expression vector in Escherichia coli.","authors":"Atif Ahmed, Nao Akusa Fujimura, Saad Tahir, Muhammad Akram, Zaheer Abbas, Maira Riaz, Ali Raza, Rabia Abbas, Nadeem Ahmed","doi":"10.1080/10826068.2024.2361146","DOIUrl":"10.1080/10826068.2024.2361146","url":null,"abstract":"Interleukin-2 has emerged as a potent protein-based drug to treat various cancers, AIDS, and autoimmune diseases. Despite its immense requirement, the production procedures are inefficient to meet the demand. Therefore, efficient production procedures must be adopted to improve protein yield and decrease procedural loss. This study analyzed cytoplasmic and periplasmic IL-2 expression for increased protein yield and significant biological activity. The study is focused on cloning IL-2 into a pET-SUMO and pET-28a vector that expresses IL-2 in soluble form and inclusion bodies, respectively. Both constructs were expressed into different E. coli expression strains, but the periplasmic and cytoplasmic expression of IL-2 was highest in overnight culture in Rosetta 2 (DE3). Therefore, E. coli Rosetta 2 (DE3) was selected for large-scale production and purification. Purified IL-2 was characterized by SDS-PAGE and western blotting, while its biological activity was determined using MTT bioassay. The results depict that the periplasmic and cytoplasmic IL-2 achieved adequate purification, yielding 0.86 and 0.51 mg/mL, respectively, with significant cytotoxic activity of periplasmic and cytoplasmic IL-2. Periplasmic IL-2 has shown better yield and significant biological activity in vitro which describes its attainment of native protein structure and function.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"45-57"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative evaluation of batch and fed-batch cultures for enhanced lipid, carotenoid, and β-carotene production by Rhodotorula mucilaginosa. 批培养和饲料批培养对提高粘液红霉菌脂质、类胡萝卜素和β-胡萝卜素产量的比较评价。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-12-24 DOI: 10.1080/10826068.2024.2444977

Gedela Ravi, Veeranki Venkata Dasu, Kannan Pakshirajan

{"title":"A comparative evaluation of batch and fed-batch cultures for enhanced lipid, carotenoid, and β-carotene production by Rhodotorula mucilaginosa.","authors":"Gedela Ravi, Veeranki Venkata Dasu, Kannan Pakshirajan","doi":"10.1080/10826068.2024.2444977","DOIUrl":"https://doi.org/10.1080/10826068.2024.2444977","url":null,"abstract":"This study explored the impact of sodium acetate (Na-acetate) impact on lipid, carotenoid, and β-carotene production by the newly isolated strain Rhodotorula mucilaginosa. Batch and fed-batch bioreactor cultures were employed to optimize growth conditions and product yields. R. mucilaginosa fed with Na-acetate in the yeast medium was evaluated in the batch bioreactor culture. The following merits were accomplished for the cell dry weight (5.02 gL-1), lipid content (65.73%), carotenoid (40.33 µgg-1) and β-carotene (17.63 µgg-1) consistently. The fed-batch reactor cultivation using yeast extract supplemented with Na-acetate yielded superior lipid content (68.58%), cell dry weight (5.92 gL-1), carotenoid (48.36 µgg-1), and β-carotene production (21.38 µgg-1) compared to batch cultivation. The fatty acid methyl esters (FAMEs) are produced from the lipids suitable for biodiesel production. These findings highlight the potential of R. mucilaginosa as a promising organism for sustainable biofuel and high-value compound production. Further optimization of culture conditions and downstream processing could enhance the commercial viability of this approach.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in ultrasound-assisted biomolecule extraction from prokaryotic and eukaryotic cells: a review. 超声辅助提取原核和真核细胞生物分子的研究进展。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-12-24 DOI: 10.1080/10826068.2024.2436952

Santosh Sethi, V K Rathod

{"title":"Recent advancements in ultrasound-assisted biomolecule extraction from prokaryotic and eukaryotic cells: a review.","authors":"Santosh Sethi, V K Rathod","doi":"10.1080/10826068.2024.2436952","DOIUrl":"https://doi.org/10.1080/10826068.2024.2436952","url":null,"abstract":"With numerous advantages over conventional techniques, ultrasound-assisted extraction (UAE) has become a viable method for the effective extraction of biomolecules from prokaryotic and eukaryotic cells. The fundamentals and workings of UAE are examined in this review, focusing on current developments, including how these impact the extraction of proteins, lipids, enzymes, and other bioactive compounds. UAE not only enhances cell disruption and mass transfer, leading to improved extraction yields, but also preserves the integrity of the extracted bioactive molecules under optimized conditions, making it a preferred choice in Biochemistry and Biotechnology. Additionally, this review explores recent innovative approaches that combine ultrasound with other techniques like enzymatic digestion, supercritical CO2, deep eutectic solvents, and Three-Phase Partitioning (UA-TPP) etc, to further enhance extraction efficiency. The differences in extraction effectiveness between prokaryotic and eukaryotic cells are attributed to cellular structure and ultrasonic conditions. Overall, this review highlights UAE's promise as a viable and efficient substitute for biomolecule extraction concerning prokaryotic and eukaryotic cells while bringing up areas that need additional research and development.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-27"},"PeriodicalIF":2.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient RNA extraction method for acquiring high-quality RNA from various tissues of the fiber crop abaca, Musa textilis Née. 用于从纤维作物巴西蕉（Musa textilis Née）的各种组织中获取高质量 RNA 的高效 RNA 提取方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-12-17 DOI: 10.1080/10826068.2024.2440421

Rhosener Bhea L Koh, Jose Planta, Richard I Encarnacion, Jasca Gayle G Española, Vermando M Aquino, Leny C Galvez

{"title":"Efficient RNA extraction method for acquiring high-quality RNA from various tissues of the fiber crop abaca, Musa textilis Née.","authors":"Rhosener Bhea L Koh, Jose Planta, Richard I Encarnacion, Jasca Gayle G Española, Vermando M Aquino, Leny C Galvez","doi":"10.1080/10826068.2024.2440421","DOIUrl":"https://doi.org/10.1080/10826068.2024.2440421","url":null,"abstract":"Isolation of high-quality RNA from abaca is very challenging due to the presence of polyphenols, polysaccharides, and its high fiber content. In this study, we compared six extraction methods across three tissue types and different developmental stages (in-vitro-grown young versus field-grown mature tissue). The Invitrogen PureLink RNA kit proved to be the most efficient in extracting RNA from young abaca tissues (leaves, pseudostem, and corm). The quality of RNA extracted from young tissues was further assessed by RNA-seq applications, with raw sequencing reads mapping back to the M. textilis reference genome at rates of 86.0%-90.4%. The SDS-TRIzol-method modified with an added on-column DNAse I treatment was used to extract RNA from mature tissues (leaves, midrib, and pseudostem). RNA isolated from five M. textilis cultivars and across three mature tissue types showed RNA yield per 100 mg of fresh weight ranges from 0.57 to 10.94 µg and RNA integrity number (RIN) scores of more than 7.0 for all tissue types. Our improved SDS-Trizol method for RNA extraction described here is simple and yields good quality RNAs from mature abaca tissues while the PureLink RNA kit is suitable for extracting RNA from young abaca samples amenable to RT-qPCR and next-generation sequencing studies.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing algal biomass for sustainable energy: cultivation, strain improvement, and biofuel production. 利用藻类生物质实现可持续能源：栽培、菌种改良和生物燃料生产。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-12-16 DOI: 10.1080/10826068.2024.2434879

Indira Mikkili, Bala Venkata Sai Teja Gaddirala, Sudarsini Borugadda, Syam Babu Davuluri

{"title":"Harnessing algal biomass for sustainable energy: cultivation, strain improvement, and biofuel production.","authors":"Indira Mikkili, Bala Venkata Sai Teja Gaddirala, Sudarsini Borugadda, Syam Babu Davuluri","doi":"10.1080/10826068.2024.2434879","DOIUrl":"https://doi.org/10.1080/10826068.2024.2434879","url":null,"abstract":"The world faces pressing environmental challenges, including greenhouse gas emissions, global warming, climate change, and rising sea levels. Alongside, these issues, the depletion of fossil fuels has intensified the search for alternative energy sources. Algal biomass presents a promising long-term solution to these global problems. The quest for sustainable energy has driven significant research into algal biofuels as a viable alternative to fossil fuels. Algae offers several advantages as a feedstock for biofuel production, including high biomass yield, rapid growth rates, cost-effective cultivation, carbon dioxide fixation capabilities, and the potential to grow on non-arable land using non-potable water. This manuscript provides an overview of algal biomass cultivation using renewable feedstocks, identifies potential algal strains for biofuel production, and explores bioengineering advancements in algae. Additionally, strain improvement strategies to enhance biofuel yields are discussed. The review also addresses large-scale algal biomass cultivation for biofuel production, assesses its commercial viability, examines challenges faced by the biofuel industry, and outlines prospects for biofuel production using highly potent algal strains. By overcoming and addressing these challenges, algal biofuels have the potential to become a cornerstone of sustainable energy solutions.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Escherichia coli to metabolize sorbitol as the sole carbon source for synthesis of recombinant L-Asparaginase-II. 改造大肠杆菌，使其代谢山梨醇作为合成重组 L-天冬酰胺酶-II的唯一碳源。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-12-13 DOI: 10.1080/10826068.2024.2440425

Dibya Ranjan Das, Shubhashree Mahalik

{"title":"Engineering Escherichia coli to metabolize sorbitol as the sole carbon source for synthesis of recombinant L-Asparaginase-II.","authors":"Dibya Ranjan Das, Shubhashree Mahalik","doi":"10.1080/10826068.2024.2440425","DOIUrl":"https://doi.org/10.1080/10826068.2024.2440425","url":null,"abstract":"Sorbitol, known as D-Glucitol, is a hexose sugar alcohol that occurs naturally in various fruits, including berries, cherries, plums, pears, and apples. It is noteworthy that sorbitol can be metabolized by microbes, plants, and humans through distinct pathways. Nevertheless, in bacteria like Escherichia coli (E. coli), sorbitol is not the primary carbon source and its utilization is generally suppressed due to carbon catabolite repression. In this context, Escherichia coli has been engineered to enable the use of sorbitol as the sole carbon source for producing recombinant proteins. This modification involves a two-plasmid system where the sorbitol-6-phosphate dehydrogenase (srlD) gene is upregulated under an araBAD promoter, while the recombinant protein is expressed from a second plasmid under the tac promoter. The overexpression of srlD in the engineered E. coli strain enhances the utilization of sorbitol as the sole carbon source. When cultured in a medium supplemented solely with sorbitol, the engineered E. coli strain exhibits a 3.6 times higher specific growth rate and yields substantially higher concentration of recombinant protein compared to the wild-type strain. Additionally, the engineered strain demonstrates a higher YP/X ratio than the wild-type strain.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pectinase immobilized on magnetic nanoparticles coated with alginate for pectin hydrolysis in guava juice assisted by a stirred electromagnetic reactor. 固定在涂有海藻酸盐的磁性纳米粒子上的果胶酶在搅拌电磁反应器的辅助下水解番石榴汁中的果胶。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-25 DOI: 10.1080/10826068.2024.2432389

Jônatas de Carvalho-Silva, Ana Cláudia Vaz de Araújo, Pedro Miguel Ferreira-Santos, Attilio Converti, Tatiana Souza Porto

{"title":"Pectinase immobilized on magnetic nanoparticles coated with alginate for pectin hydrolysis in guava juice assisted by a stirred electromagnetic reactor.","authors":"Jônatas de Carvalho-Silva, Ana Cláudia Vaz de Araújo, Pedro Miguel Ferreira-Santos, Attilio Converti, Tatiana Souza Porto","doi":"10.1080/10826068.2024.2432389","DOIUrl":"https://doi.org/10.1080/10826068.2024.2432389","url":null,"abstract":"Aspergillus aculeatus pectinase was immobilized on magnetic nanoparticles coated with calcium alginate for pectin hydrolysis in guava juice by a stirred electromagnetic reactor (SER). The average crystallite size estimated by the Scherrer formula was 33.7 nm. The reaction rate in SER (701.7 U/mL) was almost twice that of the static process (362.5 U/mL). Both processes displayed a sigmoidal trend with positive cooperativity (n) of 5 and 4, respectively. Both free and immobilized pectinase showed great performance in the pH range of 4.0-7.0. After immobilization, pectinase acted optimally at 50 °C. Pectin hydrolysis was performed for over 10 successive cycles in SER losing only 30% of its initial activity. Thermodynamic activation parameters of the reaction revealed higher spontaneity and efficiency when hydrolysis was performed in SER. Pectin hydrolysis in guava juice displayed 41% turbidity and 85.5% viscosity reduction. The electromagnetic reactor displayed great potential for performing hydrolysis of pectin in guava juice. The biocatalyst showed good features for further applications in food industries.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convenient production of a novel recombinant antibody against periodontitis biomarker S100A8. 便捷地制备针对牙周炎生物标志物 S100A8 的新型重组抗体。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-20 DOI: 10.1080/10826068.2024.2430615

Hui-Seon Yun, Eun-Jung Kim, Byung-Gee Kim, Hee-Jin Jeong

引用次数: 0

Statistical optimization and sequential scale-up of α-galactosidase production by Actinoplanes utahensis B1 from shake flask to pilot scale. 从摇瓶到中试规模，放线菌 B1 生产 α-半乳糖苷酶的统计优化和顺序放大。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-07 DOI: 10.1080/10826068.2024.2344500

D John Babu, K Balumahendra, T C Venkateswarulu, T Sathish

{"title":"Statistical optimization and sequential scale-up of α-galactosidase production by Actinoplanes utahensis B1 from shake flask to pilot scale.","authors":"D John Babu, K Balumahendra, T C Venkateswarulu, T Sathish","doi":"10.1080/10826068.2024.2344500","DOIUrl":"10.1080/10826068.2024.2344500","url":null,"abstract":"α-Galactosidase (α-GAL) is a class of hydrolase that releases galactose from galacto-oligosaccharides and synthetic substrates such as pNPG. In this study, the production of α-GAL by Actinoplanes utahensis B1 in submerged fermentation was enhanced by using statistical methods. The effects of temperature, pH, and inoculum percentage on enzyme secretion were optimized using BBD of RSM. The optimized process was scaled up from the shake flask to the laboratory scale (5 L) and to pilot scale (30 L) using KLa based scale-up strategy. By using BBD, a maximum yield of 62.5 U/mL was obtained at a temperature of 28 °C, a pH of 6.9, and an inoculum of 6.4%. Scale-up was performed successfully and achieved a yield of 74.4 U/mL and 76.8 U/mL in laboratory scale and pilot scale fermenters. The TOST was performed to validate the scale-up strategy and the results showed a confidence level of 95% for both scales indicating the perfect execution of scale-up procedure. Through the implementation of BBD and scale-up strategy, the overall enzyme yield has been significantly increased to 76%. This is the first article to explore the scale-up of α-GAL from the A. utahensis B1 strain and provide valuable insights for industrial applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1216-1225"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140877104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimization by response surface methodology for the enhanced production of rMBSP from Pichia pastoris and study of its application. 利用响应面方法优化 Pichia pastoris 的 rMBSP 生产及其应用研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-06-07 DOI: 10.1080/10826068.2024.2361159

Ting Li, Wenbo Li, Yao Guo, Long Han, Wen Zhang, Ronghui Liu, Hanqing Feng

{"title":"An optimization by response surface methodology for the enhanced production of rMBSP from Pichia pastoris and study of its application.","authors":"Ting Li, Wenbo Li, Yao Guo, Long Han, Wen Zhang, Ronghui Liu, Hanqing Feng","doi":"10.1080/10826068.2024.2361159","DOIUrl":"10.1080/10826068.2024.2361159","url":null,"abstract":"Background: Recombinant myofibril-bound serine proteinase (rMBSP) was successfully expressed in Pichia pastoris GS115 in our laboratory. However, low production of rMBSP in shake flask constraints further exploration of properties.Methods: A 5-L high cell density fermentation was performed and the fermentation medium was optimized. Response surface methodology (RSM) was used to optimize the culture condition through modeling three selected parameter.Results: Under the optimized culture medium (LBSM, 1% yeast powder and 1% peptone) and culture conditions (induction pH 5.5, temperature 29 °C, time 40 h), the yield of rMBSP was 420 mg/L in a 5-L fermenter, which was a 6-fold increase over thar, expressed in flask cultivation. The desired enzyme was purified by two-step, which yielded a 33.7% recovery of a product that had over 85% purity. The activity of purified rMBSP was significantly inhibited by Ca2+, Mg2+, SDS, guanidine hydrochloeide, acetone, isopropanol, chloroform, n-hexane and n-heptane. Enzymatic analysis revealed a Km of 2.89 ± 0.09 μM and a Vmax of 14.20 ± 0.12 nM•min-1 for rMBSP. LC-MS/MS analysis demonstrated the specific cleavage of bovine serum albumin by rMPSP.Conclusion: These findings suggest that rMPSP has potential as a valuable enzyme for protein science research.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1320-1328"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0