Preparative Biochemistry & Biotechnology最新文献_第9页

A comparative study on the influence of equipment design on the efficiency of dynamic maceration of Azadirachta excelsa leaves. 设备设计对印楝叶动态浸渍效率影响的对比研究。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-07-01 Epub Date: 2023-11-21 DOI: 10.1080/10826068.2023.2282529

Kahviyaah Letchumanan, Nor Hayati Abdullah, Azila Abdul-Aziz

{"title":"A comparative study on the influence of equipment design on the efficiency of dynamic maceration of Azadirachta excelsa leaves.","authors":"Kahviyaah Letchumanan, Nor Hayati Abdullah, Azila Abdul-Aziz","doi":"10.1080/10826068.2023.2282529","DOIUrl":"10.1080/10826068.2023.2282529","url":null,"abstract":"Dynamic maceration facilitates diffusion in solid-liquid extraction through controlling temperature and providing agitation. However, equipment design for dynamic maceration in previous investigations resulted in inadequate homogeneity of temperature and solid dispersion. A laboratory scale extractor was designed to aid the heat and mass transfer process while preventing solvent vaporization when performing dynamic maceration in a controlled environment. This study aimed to evaluate the efficiency of dynamic maceration using the laboratory scale extractor compared to a shaker incubator to extract triterpenoid saponins from Azadirachta excelsa leaves. The dynamic maceration of A. excelsa leaves was optimized using a Face-centered central composite design (FCCCD) with response surface methodology (RSM). Independent variables analyzed include ethanol-to-chloroform ratio, extraction temperature, extraction time, and sample-to-solvent ratio, while responses include yield of extract and triterpenoid saponins content (TSC). Optimum conditions were ethanol-to-chloroform ratio of 90:10, extraction temperature of 45 °C, extraction time of 60 minutes, and sample-to-solvent ratio of 1:50 g/ml. There was a significant percentage of increase in yield of extract and TSC by 41.1% and 13.3%, respectively, for the laboratory scale extractor compared to the shaker incubator. This study showed the importance of equipment design in enhancing triterpenoid saponins extraction through elevating the efficiency of the dynamic maceration process.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"749-763"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fractional mixture design: a novel design and methodology for analyzing and optimizing mixtures of several ingredients. 分数混合物设计：分析和优化多种成分混合物的新型设计和方法。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-07-01 Epub Date: 2024-01-19 DOI: 10.1080/10826068.2023.2297679

José Luis Navarrete Bolaños

{"title":"Fractional mixture design: a novel design and methodology for analyzing and optimizing mixtures of several ingredients.","authors":"José Luis Navarrete Bolaños","doi":"10.1080/10826068.2023.2297679","DOIUrl":"10.1080/10826068.2023.2297679","url":null,"abstract":"Mixture designs are employed to systematically change the composition of mixtures and investigate how those changes impact their properties. However, all mixture designs currently available are impractical for analyzing mixtures with relatively large numbers of ingredients. In response, this article presents a novel solution that builds on the construction of a new experimental design called \"fractional mixture design\". The design involves screening the ingredients in mixtures and enables the subsequent construction of a classical mixture design for optimizing mixtures. The design and its accompanying methodology were developed to analyze native strains found in successful spontaneous fermentations with the goal of constructing a mixed starter culture to transition from spontaneous to directed fermentation in the production of agave distillates. The results showed that a starter culture composed of the native strains Kluyveromyces marxianus, Clavispora lusitaniae, and Kluyveromyces marxianus var. drosophilarum, in respective proportions of 35%, 32%, and 33%, enabled the production of a fermented product with 2.1% alcohol and a broad profile of aromatic compounds. Hence, the results show, for the first time, a tool that addresses the technical challenge that allows studying a relatively large number of ingredients in mixtures and a two-stage sequential methodology to construct optimal mixtures.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"849-857"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of liquid fermentation conditions for Coprinus comatus to enhance antioxidant activity. 优化 Coprinus comatus 的液体发酵条件以提高抗氧化活性。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-07-01 Epub Date: 2023-12-26 DOI: 10.1080/10826068.2023.2297703

Dongyu Gu, Shanshan Tang, Chang Liu, Dajun He, Jing Tian, Yi Yang

{"title":"Optimization of liquid fermentation conditions for Coprinus comatus to enhance antioxidant activity.","authors":"Dongyu Gu, Shanshan Tang, Chang Liu, Dajun He, Jing Tian, Yi Yang","doi":"10.1080/10826068.2023.2297703","DOIUrl":"10.1080/10826068.2023.2297703","url":null,"abstract":"Coprinus comatus is an edible and medicinal fungus. In this study, the antioxidant activity of the fermentation product of C. comatus was investigated through optimization of fermentation process. The results indicated that the fermentation product of C. comatus had obvious scavenging ability for 2,2'-Azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) free radical. The EC50 of the n-butanol extract from the fermentation product on ABTS·+ was 0.65 ± 0.02 mg/mL. On this basis, the liquid fermentation conditions of C. comatus were optimized through single factor and response surface optimization experiments according to the scavenging ability of ABTS·+ to improve the antioxidant capacity of the fermentation product. The results showed that when the 14% of C. comatus was fermented in a culture medium with a C/N ratio of 48:1 for 6 days, the ABTS·+ scavenging ability was the strongest, and the EC50 of n-butanol extract was 0.57 ± 0.01 mg/mL, which was 12.31% higher than the initial activity. This study laid the foundation for the development of C. comatus.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"830-837"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of phytase production by Penicillium oxalicum in solid-state fermentation for potential as a feed additive. 优化草酸青霉在固态发酵中生产植酸酶的过程，以挖掘其作为饲料添加剂的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-07-01 Epub Date: 2023-12-28 DOI: 10.1080/10826068.2023.2297688

Priya, Bijender Singh, Jai Gopal Sharma, Bhoopander Giri

{"title":"Optimization of phytase production by Penicillium oxalicum in solid-state fermentation for potential as a feed additive.","authors":"Priya, Bijender Singh, Jai Gopal Sharma, Bhoopander Giri","doi":"10.1080/10826068.2023.2297688","DOIUrl":"10.1080/10826068.2023.2297688","url":null,"abstract":"The study aims to statistically optimize the phytase production by Penicillium oxalicum PBG30 in solid-state fermentation using wheat bran as substrate. Variables viz. pH, incubation days, MgSO4, and Tween-80 were the significant parameters identified through the Plackett-Burman design (PBD) that majorly influenced the phytase production. Further, central composite design (CCD) method of response surface methodology (RSM) defined the optimum values for these factors i.e., pH 7.0, 5 days of incubation, 0.75% of MgSO4, and 3.5% of Tween-80 that leads to maximum phytase production of 475.42 U/g DMR. Phytase production was also sustainable in flasks and trays of different sizes with phytase levels ranging from 394.95 to 475.42 U/g DMR. Enhancement in phytase production is 5.6-fold as compared to unoptimized conditions. The in-vitro dephytinization of feed showed an amelioration in the nutritive value by releasing inorganic phosphate and other nutrients in a time-dependent manner. The highest amount of inorganic phosphate (33.986 mg/g feed), reducing sugar (134.4 mg/g feed), and soluble protein (115.52 mg/g feed) was achieved at 37 °C with 200 U of phytase in 0.5 g feed for 48 h. This study reports the economical and large-scale production of phytase with applicability in enhancing feed nutrition.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"819-829"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139049161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of yellow fever virus through tailored culture media optimization. 通过量身定制的培养基优化提高黄热病病毒的产量。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-26 DOI: 10.1080/10826068.2024.2366990

Hareesh Reddy Narreddy, Ratna Prakash Kondapalli, T C Venkateswarulu

{"title":"Enhanced production of yellow fever virus through tailored culture media optimization.","authors":"Hareesh Reddy Narreddy, Ratna Prakash Kondapalli, T C Venkateswarulu","doi":"10.1080/10826068.2024.2366990","DOIUrl":"https://doi.org/10.1080/10826068.2024.2366990","url":null,"abstract":"In the present study, an initial screening was conducted using 12 types of cell culture media, and four media with the best performance were selected for further study. The optimization of four media blend for YFV production was evaluated using an Augmented simplex centroid mixture design. Among all the different models that were investigated, the quadratic model was found to be the most appropriate model for exploring mixture design. It was found that M10 exhibited the greatest impact on YFV production, followed by M9, M4, and M1. The utilization of M1 and M4 media individually yielded higher compared to their blends with other media. The YFV titers were reduced when M1 media was combined with other media. The utilization of M9 and M10 media in combination resulted a higher viral yield compared to their respective concentrations. The optimal ratio for achieving a higher titer of YFV from primary CEFs was found to be approximately 38:62, with M9 and M10 being the most favorable media blend. The use of a media mixture led to a significant increase of virus titer up to 2.6 × 108 PFU/ml or 2 log titer yield, which is equivalent to 1.92 × 105 doses, without any changes to growth conditions or other process factors. This study concluded that the utilization of a mixture design could be efficiently employed to choose the optimal combination of media blends for enhanced viral production from cell culture.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-6"},"PeriodicalIF":2.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141451260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteases, a powerful biochemical tool in the service of medicine, clinical and pharmaceutical. 蛋白酶是为医学、临床和制药服务的强大生化工具。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-22 DOI: 10.1080/10826068.2024.2364234

Ghadir A Jamal, Ehsan Jahangirian, Michael R Hamblin, Hamed Mirzaei, Hossein Tarrahimofrad, Neda Alikowsarzadeh

{"title":"Proteases, a powerful biochemical tool in the service of medicine, clinical and pharmaceutical.","authors":"Ghadir A Jamal, Ehsan Jahangirian, Michael R Hamblin, Hamed Mirzaei, Hossein Tarrahimofrad, Neda Alikowsarzadeh","doi":"10.1080/10826068.2024.2364234","DOIUrl":"https://doi.org/10.1080/10826068.2024.2364234","url":null,"abstract":"Proteases, enzymes that hydrolyze peptide bonds, have various applications in medicine, clinical applications, and pharmaceutical development. They are used in cancer treatment, wound debridement, contact lens cleaning, prion degradation, biofilm removal, and fibrinolytic agents. Proteases are also crucial in cardiovascular disease treatment, emphasizing the need for safe, affordable, and effective fibrinolytic drugs. Proteolytic enzymes and protease biosensors are increasingly used in diagnostic and therapeutic applications. Advanced technologies, such as nanomaterials-based sensors, are being developed to enhance the sensitivity, specificity, and versatility of protease biosensors. These biosensors are becoming effective tools for disease detection due to their precision and rapidity. They can detect extracellular and intracellular proteases, as well as fluorescence-based methods for real-time and label-free detection of virus-related proteases. The active utilization of proteolytic enzymatic biosensors is expected to expand significantly in biomedical research, in-vitro model systems, and drug development. We focused on journal articles and books published in English between 1982 and 2024 for this study.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-25"},"PeriodicalIF":2.0,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulase production by Aspergillus fumigatus A4112 and the potential use of the enzyme in cooperation with surfactant to enhance floating oil recovery and methane production from palm oil mill effluent. 烟曲霉 A4112 产生的纤维素酶以及该酶与表面活性剂合作用于提高棕榈油厂污水的浮油回收率和甲烷产量的潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-22 DOI: 10.1080/10826068.2024.2368627

Wiyada Khangkhachit, Wasana Suyotha, Sompong O-Thong, Poonsuk Prasertsan

{"title":"Cellulase production by Aspergillus fumigatus A4112 and the potential use of the enzyme in cooperation with surfactant to enhance floating oil recovery and methane production from palm oil mill effluent.","authors":"Wiyada Khangkhachit, Wasana Suyotha, Sompong O-Thong, Poonsuk Prasertsan","doi":"10.1080/10826068.2024.2368627","DOIUrl":"https://doi.org/10.1080/10826068.2024.2368627","url":null,"abstract":"This research performed cellulase production by Aspergillus fumigatus A4112 and evaluated its potential use in palm oil mill effluent (POME) hydrolysis to recover oil simultaneously with the generation of fermentable sugar useful for biofuel production under non-sterilized conditions. Empty fruit bunch (EFB) without pretreatment was used as carbon source. The combination of nitrogen sources facilitated CMCase production. The maximum activity (3.27 U/mL) was obtained by 1.0 g/L peptone and 1.5 g/L (NH4)2SO4 and 20 g/L EFB at 40 °C for 7 days. High level of FPase activity (39.51 U/mL) was also obtained. Interestingly, the enzyme retained its cellulase activities more than 60% at ambient temperature over 15 days. In enzymatic hydrolysis, Triton X-100 was an effective surfactant to increase total oil recovery in the floating form. High yield of reducing sugar (50.13 g/L) and 21% (v/v) of floating oil was recoverable at 65 °C for 48 h. Methane content of the raw POME increased from 41.49 to 64.94% by using de-oiled POME hydrolysate which was higher than using the POME hydrolysate (59.82%). The results demonstrate the feasibility of the constructed process for oil recovery coupled with a subsequent step for methane yield enhancement in biogas production process that benefits the palm oil industry.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative studies on modeling and optimization of fermentation process conditions for fungal asparaginase production using artificial intelligence and machine learning techniques 利用人工智能和机器学习技术对真菌天冬酰胺酶生产发酵工艺条件建模和优化的比较研究

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-19 DOI: 10.1080/10826068.2024.2367692

Gurunathan Baskar, Rajendran Sivakumar, Seifedine Kadry, Cheng-Di Dong, Reeta Rani Singhania, Ramanujam Praveenkumar, Elumalai Raja Sathendra

引用次数: 0

Effects of fermentation on the structures of yellow compounds in citrus pomace. 发酵对柑橘渣中黄色化合物结构的影响。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-10 DOI: 10.1080/10826068.2024.2362794

Dan-Dan Yang, Wen-Jie Li, Sheng-Jiao Lei, Hai-Yan Liu, Nong-Fei Ouyang, Jun-Dong Zhu

引用次数: 0

Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus. 嗜热革兰霉菌（Geobacillus kaustophilus）中一种恒温单酰基甘油脂肪酶的表达和特征。

IF 2.9 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-06-04 DOI: 10.1080/10826068.2024.2361147

Noriyuki Doukyu, Hayato Ito, Kugako Sugimoto

{"title":"Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus.","authors":"Noriyuki Doukyu, Hayato Ito, Kugako Sugimoto","doi":"10.1080/10826068.2024.2361147","DOIUrl":"https://doi.org/10.1080/10826068.2024.2361147","url":null,"abstract":"Thermophilic Geobacillus kaustophilus HTA426 genome possesses a monoacylglycerol lipase (MAGL) gene. MAGLs can synthesize emulsifiers for use in the food and pharmaceutical industries from fatty acids and glycerol. They can also be used to analyze monoacylglycerol (MAG) levels in serum and food. The MAGL gene from strain HTA426 was artificially synthesized and heterologously expressed in Escherichia coli BL21(DE3). The recombinant His-tag fused MAGL (GkMAGL) was purified using a Ni2+-affinity column. The purified enzyme showed a temperature optimum at 65 °C and was stable up to 75 °C after 30 min incubation. In addition, the enzyme exhibited a pH optimum of 7.5 and was stable from pH 5.0 to 11.0. The enzyme hydrolyzed monoacylglycerols and showed the highest activity toward 1-monolauroylglycerol. The enzyme was stable in the presence of various organic solvents and detergents. The addition of Triton X-100 significantly increased GkMAGL activity. The thermal stability of the enzyme was higher than that of thermostable MAGL from Geobacillus sp. 12AMOR1 (12AMOR1_MAGL). Circular dichroism spectral analysis showed that the conformational stability of the GkMAGL was higher than that of 12AMOR1_MAGL at higher temperatures. These results indicate that the GkMAGL has useful features that can be used for various biotechnological applications.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":2.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0