Preparative Biochemistry & Biotechnology最新文献_第8页

Integrated formic acid and deep eutectic solvent mediated sustainable synthesis of cellulose nanocrystals from Sterculia foetida shells. 以甲酸和深共晶溶剂为介质，从 Sterculia foetida 贝壳中持续合成纤维素纳米晶体。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-30 DOI: 10.1080/10826068.2024.2419873

Kurappalli Rohil Kumar, Nirajha Vishnu, Gnanabarathi C S, Kiran Babu Uppuluri, Rangabhashiyam Selvasembian

{"title":"Integrated formic acid and deep eutectic solvent mediated sustainable synthesis of cellulose nanocrystals from Sterculia foetida shells.","authors":"Kurappalli Rohil Kumar, Nirajha Vishnu, Gnanabarathi C S, Kiran Babu Uppuluri, Rangabhashiyam Selvasembian","doi":"10.1080/10826068.2024.2419873","DOIUrl":"https://doi.org/10.1080/10826068.2024.2419873","url":null,"abstract":"The present study reports the green synthesis of cellulose nanocrystals from the shells of Sterculia foetida (SFS) cellulose. Three different methods, alkali, acid and organic acid, were screened for the maximum cellulose extraction. A maximum cellulose yield, 30.6 ± 0.84 w/w, was obtained using 90% formic acid at 110 °C in 120 min. The extracted cellulose was characterized and identified by instrumental analyses. SEM analysis showed skeletal rod-like microfibril structures and similar intra-fibrillar widths. CP/MAS 13C NMR and FTIR spectrum revealed the purity of cellulose and the absence of other components like hemicellulose and lignin. XRD study revealed a cellulose crystallinity index of 88.07%. BET analysis showed a good surface area (3.3213 m2/g) and a micro-pore area of 1.871 m2/g. The cellulose nanocrystals were synthesized from the extracted cellulose using deep eutectic solvents (DES), choline chloride and lactic acid (1:2 ratio). The cellulose nanocrystals (CNC) synthesized from DES-based exhibited zeta potential and particle size of -16.7 mV and 576.3 d.nm. DES-synthesized cellulose nanocrystals were spherical-like shapes, as observed from TEM images. The present results exposed that formic acid is an effective and green catalyst for the extraction of cellulose and DES for the sustainable synthesis of CNC.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variability in growth and biosynthetic activity of Calendula officinalis hairy roots. 金盏花毛根的生长和生物合成活性的变化。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-21 DOI: 10.1080/10826068.2024.2418015

Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk

{"title":"Variability in growth and biosynthetic activity of Calendula officinalis hairy roots.","authors":"Nadiia Matvieieva, Taisa Bohdanovych, Valeriia Belokurova, Volodymyr Duplij, Anatolii Shakhovsky, Dmytro Klymchuk, Mykola Kuchuk","doi":"10.1080/10826068.2024.2418015","DOIUrl":"https://doi.org/10.1080/10826068.2024.2418015","url":null,"abstract":"Calendula officinalis is a widespread medicinal plant with a sufficiently well-studied chemical composition. Secondary metabolites synthesized by C.officinalis plants have pharmacological value for treating numerous diseases, and various types of aseptic in vitro cultures can be used as a source of these compounds. From this perspective, hairy roots attract considerable attention for the production of bioactive chemicals, including flavonoids with antioxidant activity. This paper shows the possibility of C.officinalis hairy roots obtaining with 100% frequency by Agrobacterium rhizogenes genetic transformation. Hairy root lines differed in growth rate and flavonoid content. In particular, flavonoids were accumulated in the amount of up to 6.68 ± 0.28 mg/g of wet weight. Methyl jasmonate in the concentration of 10 µM inhibited root growth to a small extent but stimulated the synthesis of flavonoids. The antioxidant activity and the reducing power increased in the roots grown in the medium with methyl jasmonate. The strong correlation of antioxidant activity and reducing power with flavonoid content was detected. The influence of extraction conditions on the content of flavonoids in the extracts and their bioactivity was determined. The potent reducing activity of extracts from hairy roots allowed the production of silver nanoparticles, which was confirmed by transmission electron microscopy.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review. 从天然资源中提取类胡萝卜素的绿色和可持续技术：综述。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-20 DOI: 10.1080/10826068.2024.2402905

Surbhi Sharma, Anshika Dedha, Manju M Gupta, Nahar Singh, Arvind Gautam, Abha Kumari

{"title":"Green and sustainable technologies for extraction of carotenoids from natural sources: a comprehensive review.","authors":"Surbhi Sharma, Anshika Dedha, Manju M Gupta, Nahar Singh, Arvind Gautam, Abha Kumari","doi":"10.1080/10826068.2024.2402905","DOIUrl":"https://doi.org/10.1080/10826068.2024.2402905","url":null,"abstract":"In recent years, driven by increasing consumer demand for natural and healthy convenient foods, the food industry has been shifting from synthetic to natural products. This shift is also reflected in the growing popularity of non-conventional extraction methods for pigments, which are favored for sustainability and environment-friendliness compared to conventional processes. This review aims to investigate the extraction of carotenoids from a variety of natural sources, including marine sources like fungus, microalgae, and crustaceans, as well as widely studied plants like tomatoes and carrots. Additionally, it delves into the recovery of valuable carotenoids from waste products like pomace and peels, highlighting the nutritional and environmental benefits. The review also emphasizes the role of green solvents such limonene, vegetable oils, ionic liquids, supercritical fluids, and natural deep eutectic solvents in effective and ecologically friendly carotenoid extraction. These technologies support the ideas of a circular and sustainable economy in addition to having a smaller negative impact on the environment. Overall, the present study highlights the crucial importance of green extraction technologies in achieving the dual goals of sustainability and public safety.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-33"},"PeriodicalIF":2.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and stability verification of reference genes in Botrytis cinerea ZX2 fermentation. 肉毒芽孢杆菌 ZX2 发酵中参考基因的筛选和稳定性验证。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-11 DOI: 10.1080/10826068.2024.2405944

Yifan Wang, Dan Shu, Zhemin Li, Di Luo, Jie Yang, Tianfu Li, Xiaonan Hou, Qi Yang, Hong Tan

{"title":"Screening and stability verification of reference genes in Botrytis cinerea ZX2 fermentation.","authors":"Yifan Wang, Dan Shu, Zhemin Li, Di Luo, Jie Yang, Tianfu Li, Xiaonan Hou, Qi Yang, Hong Tan","doi":"10.1080/10826068.2024.2405944","DOIUrl":"10.1080/10826068.2024.2405944","url":null,"abstract":"Botrytis cinerea, an airborne plant pathogen, holds the potential to synthesize sesquiterpenes, which have been used for the industrial production of abscisic acid. Previously, through our genetic technology, we obtained strain ZX2, whose main product 1´,4´-trans-ABA-diol is physiologically active in plants. In this study, 50 L of fed fermentation was carried out with ZX2 strain to study the stability of expression of TUA, TUB, ATC, EF-1, GAPDH, UCE and GTP genes. Four kinds of software (GeNorm, NormFinder, BestKeeper and Delta Ct) were used to analyze the expression stability of candidate genes, and finally the best reference gene was screened by RefFinder. Based on the results, the ACT was the most stable gene. It was used to normalize the expression levels of two genes related to 1´,4´-trans-ABA-diol production (hmgr and bcaba3) when fed-batch fermentation. Guide the selection of appropriate internal reference genes during the fermentation process to accurately quantify the relative transcription levels of target genes in B.cinerea ZX2.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-8"},"PeriodicalIF":2.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains. 蜜蜂分离的 Meyerozyma caribbica 菌株产生乙醇和 2-苯基乙醇。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-10 DOI: 10.1080/10826068.2024.2414094

Eduardo Dias Fenner, Stéfany Kell Bressan, Angela Alves Dos Santos, Anderson Giehl, Gabriel do Amaral Minussi, Elisa Amorim Amâncio Teixeira, Mariana da Costa Diniz, Larissa Werlang, Odinei Fogolari, Carlos Augusto Rosa, Helen Treichel, Liziara da Costa Cabrera, Sergio Luiz Alves Junior

{"title":"Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains.","authors":"Eduardo Dias Fenner, Stéfany Kell Bressan, Angela Alves Dos Santos, Anderson Giehl, Gabriel do Amaral Minussi, Elisa Amorim Amâncio Teixeira, Mariana da Costa Diniz, Larissa Werlang, Odinei Fogolari, Carlos Augusto Rosa, Helen Treichel, Liziara da Costa Cabrera, Sergio Luiz Alves Junior","doi":"10.1080/10826068.2024.2414094","DOIUrl":"https://doi.org/10.1080/10826068.2024.2414094","url":null,"abstract":"Investigating the biotechnological potential of wild microorganisms is paramount for optimizing bioprocesses. Given this premise, we looked for yeasts in Brazilian native stingless bees, considering the recognized potential of pollinating insect-associated microorganisms for the production of volatile organic compounds (VOCs). Two yeast strains of the species Meyerozyma caribbica were isolated from bees Scaptotrigona postica and evaluated for their fermentative capacity. Both yeasts were capable of fermenting sucrose (the main sugar used in the Brazilian ethanol industry) with over 90% efficiency and yields of up to 0.504 g/g. Through an experimental design analysis (CCD), it was verified that the ethanol productivity of these yeasts can also benefit from high concentrations of sucrose and low pH values, desirable traits for microorganisms in this biofuel production. At the same time, CCD analyses also showed the great capacity of these M. caribbica strains to produce another alcohol of broad biotechnological interest, 2-phenylethanol. Interestingly, the statistical analyses demonstrated that greater production of this compound can occur at high sugar concentrations and low availability of nitrogen sources, which can be easily achieved using residual low-cost feedstocks. Thus, our results suggest that these M. caribbica strains may be efficiently used in both ethanol and 2-phenylethanol production.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel. 最大限度地提高多酚产量：芒果皮的超声波辅助提取和抗菌潜力。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-02 DOI: 10.1080/10826068.2024.2411518

Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri

{"title":"Maximizing polyphenol yield: ultrasound-assisted extraction and antimicrobial potential of mango peel.","authors":"Md Shohag Sarker, Md Moktadirul Alam, Chen Jiao, Wu Shuqi, Li Xiaohui, Nasir Ali, L O Mallasiy, Azizah A Alshehri","doi":"10.1080/10826068.2024.2411518","DOIUrl":"https://doi.org/10.1080/10826068.2024.2411518","url":null,"abstract":"This study investigated the ultrasound-assisted extraction (UAE) techniques used to enhance the polyphenols and antioxidants of mango peel extract (MPE). Additionally, it explored the bacteriostatic activity of MPE against various microorganisms. The UAE method was optimized using response surface methodology (RSM) at different times, temperatures, and ratios, with optimal conditions found to be 35 minutes, 45 °C, and a 1:35 ratio. The optimized yield results for total polyphenol content (TPC) were 17.33 ± 1.57 mg GAE/g, total flavonoid content (TFC) was 12.14 ± 0.29 mg QE/g, and radical scavenging activity (RSA) was 72.11 ± 2.19%. These response models were extremely significant with p-values less than 0.05. MPE showed selective effectiveness against Bacillus cereus, Geobacillus stearothermophilus, and Escherichia coli (E. coli). The results highlight the potential of mango peel as a sustainable source of bioactive compounds, contributing to waste reduction in the food industry and the development of natural antimicrobial agents. This study contributes to further research on the application of MPE in processed foods.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of membrane separation technology in the purification of pharmaceutical components. 膜分离技术在药物成分纯化中的应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-25 DOI: 10.1080/10826068.2024.2328673

Yun Bi, Jingyi Dong, Yujia Zhou, Manyue Zhang, Xingying Chen, Yuyan Zhang

引用次数: 0

Microwave-enhanced hydrolysis of cellulose by acidic ionic liquids. 微波增强酸性离子液体对纤维素的水解作用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-23 DOI: 10.1080/10826068.2024.2333467

Meng-Meng Liu, Liang-Yan Zhang, Zhen Liu

引用次数: 0

Statistical versus neural network-embedded swarm intelligence optimization of a metallo-neutral-protease production: activity kinetics and food industry applications. 金属中性蛋白酶生产的统计与神经网络嵌入式群集智能优化：活性动力学与食品工业应用。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-03-16 DOI: 10.1080/10826068.2024.2328681

Maurice George Ekpenyong, Sylvester Peter Antai

{"title":"Statistical versus neural network-embedded swarm intelligence optimization of a metallo-neutral-protease production: activity kinetics and food industry applications.","authors":"Maurice George Ekpenyong, Sylvester Peter Antai","doi":"10.1080/10826068.2024.2328681","DOIUrl":"10.1080/10826068.2024.2328681","url":null,"abstract":"An integrated approach involving response surface methodology (RSM) and artificial neural network-ant-colony hybrid optimization (ANN-ACO) was adopted to develop a bioprocess medium to increase the yield of Bacillus cereus neutral protease under submerged fermentation conditions. The ANN-ACO model was comparatively superior (predicted r2 = 98.5%, mean squared error [MSE] = 0.0353) to RSM model (predicted r2 = 86.4%, MSE = 23.85) in predictive capability arising from its low performance error. The hybrid model recommended a medium containing (gL-1) molasses 45.00, urea 9.81, casein 25.45, Ca2+ 1.23, Zn2+ 0.021, Mn2+ 0.020, and 4.45% (vv-1) inoculum, for a 6.75-fold increase in protease activity from a baseline of 76.63 UmL-1. Yield was further increased in a 5-L bioreactor to a final volumetric productivity of 3.472 mg(Lh)-1. The 10.0-fold purified 46.6-kDa-enzyme had maximum activity at pH 6.5, 45-55 °C, with Km of 6.92 mM, Vmax of 769.23 µmolmL-1 min-1, kcat of 28.49 s-1, and kcat/Km of 4.117 × 103 M-1 s-1, at 45 °C, pH 6.5. The enzyme was stabilized by Ca2+, activated by Zn2+ but inhibited by EDTA suggesting that it was a metallo-protease. The biomolecule significantly clarified orange and pineapple juices indicating its food industry application.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1132-1146"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization. 利用甘蔗渣的价值，通过土曲霉高产生产漆酶，有效去除偶氮染料。

IF 2 4区生物学

Preparative Biochemistry & Biotechnology Pub Date : 2024-10-01 Epub Date: 2024-04-01 DOI: 10.1080/10826068.2024.2332881

T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu

{"title":"Valorization of sugarcane bagasse for high-yield production of laccase through Aspergillus terreus for effective azo dye decolourization.","authors":"T Chandukishore, Tuhin Subhra Biswas, Ashish A Prabhu","doi":"10.1080/10826068.2024.2332881","DOIUrl":"10.1080/10826068.2024.2332881","url":null,"abstract":"Synthetic dyes such as azo dyes are significant pollutants in the wastewater released from various textile industries. The low biodegradability and production from synthetic sources with high shelf life make azo dyes a challenging material for degradation. This study used chemically mutated Aspergillus terrus in the laccase production under solid-state fermentation using sugarcane bagasse. Initially, the wild-type strain produced a laccase activity of 4.12 U/mL. Later, the alkaline pretreatment of sugarcane bagasse showed a significant increase in laccase activity by 38.9%. Further, random mutagenesis treatment with 100 mM EMS generated a hyper laccase-producing strain with a 2.3-fold increment in laccase activity compared to the wild-type strain. The enzyme displayed optimal activity at pH 6.5 and 35 °C. The metal ions such as Fe3+ (29.4 U/mL), Fe2+ (20.8 U/mL) and Cu2+ (18.05 U/mL) showed positive effects on laccase activity. The crude laccase was used to bioremediate Congo red, a prominent azo dye used in textile and pharmaceutical industries. The preliminary studies with a crude enzyme displayed 68.86% dye decolourization after 24 h of incubation. Additionally, with Taguchi orthogonal array optimization experiments, the maximal dye decolorization of 78.24% was achieved by maintaining crude enzyme concentration (20 U), dye concentration (25 mg/L) and pH 4.5.","PeriodicalId":20401,"journal":{"name":"Preparative Biochemistry & Biotechnology","volume":" ","pages":"1170-1181"},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0